CN106226371A - The preparation of cadmium sulfide cladding ceria hollow ball detection tumor cell sensor - Google Patents
The preparation of cadmium sulfide cladding ceria hollow ball detection tumor cell sensor Download PDFInfo
- Publication number
- CN106226371A CN106226371A CN201610537707.5A CN201610537707A CN106226371A CN 106226371 A CN106226371 A CN 106226371A CN 201610537707 A CN201610537707 A CN 201610537707A CN 106226371 A CN106226371 A CN 106226371A
- Authority
- CN
- China
- Prior art keywords
- solution
- hollow ball
- cadmium sulfide
- cladding
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Nanotechnology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Electrochemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
Cadmium sulfide cladding ceria hollow ball detects the preparation method of tumor cell sensor and is used for the method that kinds of tumor cells detects, and relates to a kind of ceria hollow ball nano composite material preparing cadmium sulfide cladding and method thereof.The present invention utilizes hydro-thermal method and two kinds of methods of electrostatic interaction to achieve the preparation of the ceria hollow ball nano composite material that cadmium sulfide is coated with, finally by the ceria hollow ball nanocomposite applications of the cadmium sulfide cladding of synthesis among the preparation of cancerous cell mark biosensor, achieve the Sensitive Detection to multiple cancerous cell mark, the method have simple to operate, response speed is fast, effective, selectivity strong, low cost and other advantages.The method can also realize the detection to kinds of tumor cells mark by the antibody type that change is modified, and can be used for scientific research aspect and clinical medicine aspect, especially has the strongest application prospect at aspects such as field of bioanalysis, tumor cell detections.
Description
Technical field
The present invention relates to a kind of ceria hollow ball nano composition preparing cadmium sulfide cladding and method thereof,
And by this materials application in the method for detection cancerous cell mark (or other target tumor).
Background technology
Ceria is a kind of important broad stopband rare earth element semi-conducting material, has good optically and electrically property
Matter, is with a wide range of applications in high-tech areas such as sensors.Ceria hollow ball nanoparticle is because of its higher ratio
Surface area and faster electron transport ability, be preferable optical material.Cadmium sulfide is the semi-conducting material that another is conventional,
There is good photoelectric property, can be used among electrogenerated chemiluminescence immunoassay.
Cancer is one of important diseases threatening human life, and intestinal cancer is one relatively conventional in cancer, the most such as
What detection in early days and diagnosis intestinal cancer become one of global study hotspot.The detection means taked inside Hospitals at Present mostly is enzyme
Linked immune analytic method, the method is by detection tumor cell mark indirect detection tumor cell, and therefore sensitivity is relatively low, difficult
With early discovery and prevent and treat.Scientist both domestic and external attempts multiple method detection cancerous cell, it is desirable to achieve improves sensitivity, reaches
Early finding the purpose of early treatment, method includes chemiluminescence method, blend-out voltammetry and chronoamperometry etc..But, above-mentioned side
Method is generally of the shortcomings such as analysis detection time length, complex operation.
Summary of the invention
The problem existed for prior art, the present invention provides the ceria hollow ball nanoparticle that a kind of cadmium sulfide is coated with
The preparation method of sub-composite and the method for highly sensitive detection cancerous cell mark based on this nano composite material, the party
Method have simple to operate, response speed is fast, effective, selectivity is high and low cost and other advantages.Important feature is that ceria is empty
Heart nanosphere has good bearing capacity and absorbability, and provides good electron transfer capacity, for efficient raw
Analyte detection is laid a good foundation.
The present invention prepare in accordance with the following steps cadmium sulfide cladding ceria hollow ball nano composition:
(1) nano Ce O2The synthesis of hollow ball material: prepare nano Ce O with hydro-thermal method2Hollow ball, 1 ~ 1.5 mmol CeCl3·
7H2O is dissolved in 40 ~ 50 mL deionized waters, then by 10 ~ 15 mmol CO (NH2)2Join with 100 ~ 110 mL
In solution.After magnetic agitation 10 ~ 20 minutes, transfer the solution in the reactor of Teflon material.Reactor is placed in 200
~ 220 ° of C baking ovens heat 18 ~ 24 hours.After having reacted, the nano material ethanol solution centrifuge washing 3 ~ 5 that will obtain
Secondary, last dry for standby.
(2) synthesis of the ceria hollow ball nano composite material of cadmium sulfide cladding: weigh dry ceria and receive
Rice corpuscles 0.02 ~ 0.05 gram, joins in 20 ~ 30 mL distilled water.This solution is stirred vigorously, is slowly added dropwise dense simultaneously
Degree is sodium sulfide solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L；After continuing to be stirred vigorously 30 ~ 40 min, it is slowly added dropwise concentration
It is chromium chloride solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L, then proceedes to be stirred vigorously 30 ~ 40 min.Finally, stop stirring,
Product is centrifuged, washs and dries, standby.
The present invention detects cancerous cell mark in accordance with the following steps:
(1) nano Ce O is prepared with hydro-thermal method2Hollow ball；
(2) synthesis of the ceria hollow ball nano composite material of cadmium sulfide cladding: weigh dry cerium dioxide nano grain
Son 0.02 ~ 0.05 gram, joins in 20 ~ 30 mL distilled water.Being stirred vigorously by this solution, be slowly added dropwise concentration is simultaneously
Sodium sulfide solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L；After continuing to be stirred vigorously 30 ~ 40 min, being slowly added dropwise concentration is
Chromium chloride solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L, then proceedes to be stirred vigorously 30 ~ 40 min.Finally, stop stirring, right
Product is centrifuged, washs and dries, standby.
(3) synthesis of golden nanometer particle: the sodium citrate solution of 1.00 ~ 1.5 mL, 1 ~ 2 wt.% is added drop-wise to 100 ~
150 mL, 0.01 ~ 0.02 wt.% gold chloride boiling solution in, return stirring reacted after 10 ~ 30 minutes, stopped heating,
Under air-proof condition, continue to stir to returning to room temperature, standby；
(4) assembling of biosensor: the ceria hollow ball nanoparticle with cadmium sulfide cladding that will obtain in step (2)
The ITO electrode deionized water rinsing of sub-composite, dries up this electrode with nitrogen；Drip 5 ~ 10 microlitres in its surface
The aqueous solution of 3-aminopropyl-triethoxysilane, be placed in nitrogen atmosphere, after 10 ~ 30 minutes, by preparation in step (3)
Good golden nanometer particle 10 ~ 20 microlitre is added drop-wise to the ITO electrode surface modified by APTES, is placed in nitrogen atmosphere, reaction
About 0.5 ~ 1 hour, tumor cell antibody is modified the surface of electrode, be placed in 37 DEG C of incubators standby；
(5) use electrogenerated chemiluminescence technology that cancerous cell is detected.
The method of the present invention focuses on utilizing hydro-thermal method one step to prepare ceria hollow ball nano material, utilizes quiet
Electro ultrafiltration achieves modification and the assembling of nano material, finally by the nanocomposite applications of synthesis in the biological biography of cancerous cell
The preparation of sensor, it is achieved that the Sensitive Detection to colon-cancer cell mark, have simple to operate, response speed is fast, effective, choosing
Selecting property is strong, low cost and other advantages.The method can also realize the inspection to multiple colon-cancer cell by the antibody type that change is modified
Surveying, can be used for scientific research aspect and clinical medicine aspect, especially at field of bioanalysis, the aspect such as tumor cell detection has the strongest
Application prospect.
Accompanying drawing explanation
Fig. 1 is colon-cancer cell mark CEA detection curve, and wherein: the logarithm value of X-axis CEA concentration, the electroluminescent chemistry of Y-axis is sent out
Light intensity value.
Detailed description of the invention
Below in conjunction with detailed description of the invention, technical scheme is further described, but limitation is not such,
Every technical solution of the present invention is modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention,
All should contain in protection scope of the present invention.
As a example by colon-cancer cell mark, based on nano combined array material the highly sensitive detection colon-cancer cell of the present invention
Method, comprise the steps:
(1) nano Ce O2The synthesis of hollow ball material: prepare nano Ce O with hydro-thermal method2Hollow ball, 1 ~ 1.5 mmol CeCl3·
7H2O is dissolved in 40 ~ 50 mL deionized waters, then by 10 ~ 15 mmol CO (NH2)2Join with 100 ~ 110 mL
In solution.After magnetic agitation 10 ~ 20 minutes, transfer the solution in the reactor of Teflon material.Reactor is placed in 200
~ 220 ° of C baking ovens heat 18 ~ 24 hours.After having reacted, the nano material ethanol solution centrifuge washing 3 ~ 5 that will obtain
Secondary, last dry for standby.
(2) synthesis of the ceria hollow ball nano composite material of cadmium sulfide cladding: weigh dry ceria and receive
Rice corpuscles 0.02 ~ 0.05 gram, joins in 20 ~ 30 mL distilled water.This solution is stirred vigorously, is slowly added dropwise dense simultaneously
Degree is sodium sulfide solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L；After continuing to be stirred vigorously 30 ~ 40 min, it is slowly added dropwise concentration
It is chromium chloride solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L, then proceedes to be stirred vigorously 30 ~ 40 min.Finally, stop stirring,
Product is centrifuged, washs and dries, standby.
(3) synthesis of golden nanometer particle: first with the porous membrane of 0.45 μm by sodium citrate solution and chlorauric acid solution mistake
Filter, then by 1.00 mL, 1% (mass concentration) sodium citrate solution and 100 mL 0.01% (mass concentration) gold chlorides
Solution is mixed in flask, after backflow (100 DEG C) stirring is reacted ten to three ten minutes, stops heating.Under air-proof condition, continue
Stirring is to returning to room temperature (25 DEG C), standby.The concrete return stirring time depends on required golden nanometer particle size.
(4) assembling of biosensor: the ceria hollow ball nanoparticle with cadmium sulfide cladding obtained in step (2) is answered
The ITO electrode deionized water rinsing of condensation material, dries up this electrode with nitrogen.Drip the 3-of 5 ~ 10 microlitres in its surface
The aqueous solution (APTES) (concentration is 1%, volume fraction) of aminopropyl-triethoxysilane.It is placed in nitrogen atmosphere, very
Zhong Hou, is added drop-wise to the ITO electrode table modified by APTES by golden nanometer particle 10 ~ 20 microlitre prepared in step (3)
Face, is placed in nitrogen atmosphere, more than half an hour.By tumor cell antibody (10-6G/mL) modify the surface of electrode, be placed in 37
In degree Celsius incubator standby.
(5) detection to colon-cancer cell mark: use electrogenerated chemiluminescence technology that colon-cancer cell mark is examined
Surveying, concrete detection method is as follows:
Utilize three-electrode system, obtain as in electrode, step 2 as reference electrode, platinum electrode including saturated calomel electrode
The electrode arrived is as working electrode.Three electrodes be concurrently placed at containing 0.1 M PBS phosphate buffer solution (pH 7.4), 0.05
M K2S2O8With in the solution of 0.1 M KCl, be to be scanned between 0 ~-1.5V in voltage range.In electrode surface, sulfuration
The zinc oxide of cadmium cladding can be with the K in solution2S2O8Coreaction, thus produce electrochemiluminescence signal.
According to step (4) and (5), by the specific immune response of cancerous cell mark CEA Yu antibody, can be according to anti-
The quantity of cancerous cell mark combined on body and the linear change of electrogenerated chemiluminescence intensity realize cancerous cell mark
Detection.Owing to the combination of cancerous cell mark CEA with antibody can produce inhibition to the electron transfer in electrode and solution,
Electrochemiluminescence signal is reduced, it is achieved thereby that the detection by quantitative to cancerous cell mark CEA.
Detection mechanism: with increasing of the cancerous cell mark CEA of antibodies, can hinder in solution and receive on electronics and electrode
Transmission effect between rice material, i.e. causes electrode impedance to be continuously increased, and then makes institute under same electric current shooting condition
The optical signal of the electrogenerated chemiluminescence produced weakens.It is, the quantity of the cancerous cell mark combined on electrode surface and electricity
There is linear relationship in the numerical value causing chemiluminescence signal reduction.Highly sensitive to cancerous cell mark is realized according to this linear relationship
Detection.
This detection method based on nano composite material is capable of the Sensitive Detection to cancerous cell mark, experiment knot
Fruit shows that the intensity of the electrochemiluminescence signal logarithm with the concentration of cancerous cell is at 0.09 pg/ml-1~1.5 ng/ml-1Scope
Interior journey linear relationship.According to linear relationship, cancerous cell mark can be carried out detection by quantitative (as shown in Figure 1), its sensitivity
Increase than existing method.
Claims (7)
1. the preparation of cadmium sulfide cladding ceria hollow ball detection tumor cell sensor, it is characterised in that described preparation method
Step is as follows:
(1) nano Ce O2The synthesis of hollow ball material: prepare nano Ce O with hydro-thermal method2Hollow ball, 1 ~ 1.5 mmol CeCl3·
7H2O is dissolved in 40 ~ 50 mL deionized waters, then by 10 ~ 15 mmol CO (NH2)2Join with 100 ~ 110 mL
In solution, magnetic agitation, after 10 ~ 20 minutes, transfers the solution in the reactor of Teflon material, reactor is placed in 200
~ 220 ° of C baking ovens heat 18 ~ 24 hours, after having reacted, the nano material ethanol solution centrifuge washing 3 ~ 5 that will obtain
Secondary, last dry for standby；
(2) synthesis of the ceria hollow ball nano composite material of cadmium sulfide cladding: weigh dry cerium dioxide nano grain
Son 0.02 ~ 0.05 gram, joins in 20 ~ 30 mL distilled water；Being stirred vigorously by this solution, be slowly added dropwise concentration is simultaneously
Sodium sulfide solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L；After continuing to be stirred vigorously 30 ~ 40 min, being slowly added dropwise concentration is
Chromium chloride solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L, then proceedes to be stirred vigorously 30 ~ 40 min；Finally, stop stirring, right
Product is centrifuged, washs and dries, standby.
The preparation method of the ceria hollow ball nano composite material of cadmium sulfide the most according to claim 1 cladding, its
It is characterised by that described hydro-thermal method prepares nano Ce O2The step of the synthesis of hollow ball material is as follows:
By 1 ~ 1.5 mmol CeCl3·7H2O is dissolved in 40 ~ 50 mL deionized waters, then by 10 ~ 15 mmol CO
(NH2)2Joining in solution with 100 ~ 110 mL, magnetic agitation, after 10 ~ 20 minutes, transfers the solution into Teflon material
Reactor in, reactor is placed in 200 ~ 220 ° of C baking ovens heating 18 ~ 24 hours, after having reacted, the nanometer that will obtain
Material ethanol solution centrifuge washing 3 ~ 5 times, last dry for standby.
The preparation method of the ceria hollow ball nano composite material of cadmium sulfide the most according to claim 2 cladding, its
It is characterised by described CeCl3·7H2O and CO (NH2)2The concentration of solution is than for 1:10.
The preparation method of the ceria hollow ball nano composite material of cadmium sulfide the most according to claim 1 cladding, its
It is characterised by that the concentration of described sodium sulfide solution and cadmium chloride solution is 0.15 mol/L.
5. the method for a highly sensitive detection cancerous cell mark based on nano composite material, it is characterised in that described method walks
Rapid as follows:
(1) synthesis of nanometer titanium dioxide cerium material: prepare ceria hollow ball nanoparticle with hydro-thermal method；
(2) synthesis of the ceria hollow ball nano composite material of cadmium sulfide cladding: weigh dry cerium dioxide nano grain
Son 0.02 ~ 0.05 gram, joins in 20 ~ 30 mL distilled water, is stirred vigorously by this solution, and be slowly added dropwise concentration is simultaneously
Sodium sulfide solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L；After continuing to be stirred vigorously 30 ~ 40 min, being slowly added dropwise concentration is
Chromium chloride solution 1 ~ 1.5 mL of 0.15 ~ 0.2 mol/L, then proceedes to be stirred vigorously 30 ~ 40 min；Finally, stop stirring, right
Product is centrifuged, washs and dries, standby；
(3) synthesis of golden nanometer particle: the sodium citrate solution of 0.5 ~ 1.2 mL, 1.0 ~ 1.5 wt.% is added drop-wise to 100 ~
150 mL, 0.01 ~ 0.03 wt.% gold chloride boiling solution in, return stirring reacted after 10 ~ 15 minutes, stopped heating,
Under air-proof condition, continue to stir to returning to room temperature, standby；
(4) assembling of biosensor: the ceria hollow ball nanometer with cadmium sulfide cladding obtained in step (2) is answered
Condensation material be added drop-wise to gold electrode surfaces, with nitrogen, this electrode is dried up；Drip the 3-aminopropan of 1 ~ 3 microlitre in its surface
The aqueous solution of base-triethoxysilane (APS), is placed in nitrogen atmosphere, after 20 ~ 30 minutes, and the gold that will prepare in step (3)
Nanoparticle 5 ~ 10 microlitre is added drop-wise to the gold electrode surfaces modified by APS, is placed in nitrogen atmosphere, and reaction 0.5 ~ 1.5 is little
Time, tumor cell antibody is modified the surface of electrode, is placed in 37 DEG C of incubators standby；
(5) use electrogenerated chemiluminescence technology that cancerous cell mark is detected.
The method of highly sensitive detection cancerous cell mark based on nano composite material the most according to claim 5, it is special
Levy and be that described hydro-thermal method prepares ceria hollow ball nano material:
By 1 ~ 1.5 mmol CeCl3·7H2O is dissolved in 40 ~ 50 mL deionized waters, then by 10 ~ 15 mmol CO
(NH2)2Joining in solution with 100 ~ 110 mL, magnetic agitation, after 10 ~ 20 minutes, transfers the solution into Teflon material
Reactor in, reactor is placed in 200 ~ 220 ° of C baking ovens heating 18 ~ 24 hours, after having reacted, the nanometer that will obtain
Material ethanol solution centrifuge washing 3 ~ 5 times, last dry for standby.
The method of highly sensitive detection cancerous cell based on nano composite material the most according to claim 5, it is characterised in that
The concentration of described sodium sulfide solution and cadmium chloride solution is 0.15 mol/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610537707.5A CN106226371A (en) | 2016-07-11 | 2016-07-11 | The preparation of cadmium sulfide cladding ceria hollow ball detection tumor cell sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610537707.5A CN106226371A (en) | 2016-07-11 | 2016-07-11 | The preparation of cadmium sulfide cladding ceria hollow ball detection tumor cell sensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106226371A true CN106226371A (en) | 2016-12-14 |
Family
ID=57519838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610537707.5A Pending CN106226371A (en) | 2016-07-11 | 2016-07-11 | The preparation of cadmium sulfide cladding ceria hollow ball detection tumor cell sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106226371A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257070A (en) * | 2019-04-28 | 2019-09-20 | 中国科学院理化技术研究所 | A kind of hollow zirconium@quantum dot composite balls and its preparation method and application |
| CN112649605A (en) * | 2020-12-14 | 2021-04-13 | 哈尔滨理工大学 | Based on NaBiF4ECL biosensor of up-conversion nano-particles |
-
2016
- 2016-07-11 CN CN201610537707.5A patent/CN106226371A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257070A (en) * | 2019-04-28 | 2019-09-20 | 中国科学院理化技术研究所 | A kind of hollow zirconium@quantum dot composite balls and its preparation method and application |
| CN110257070B (en) * | 2019-04-28 | 2022-03-04 | 中国科学院理化技术研究所 | Hollow zirconia @ quantum dot composite ball and preparation method and application thereof |
| CN112649605A (en) * | 2020-12-14 | 2021-04-13 | 哈尔滨理工大学 | Based on NaBiF4ECL biosensor of up-conversion nano-particles |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104133069B (en) | Preparation method for double-functional mark photo-electrochemical sensor and application | |
| CN108802133B (en) | A kind of preparation method and application detecting stomach neoplasms tumor markers interlayer type immunosensor | |
| CN104880456B (en) | A kind of based on GO/MWCNTs-COOH/Au@CeO2the preparation method and application of the electrochemiluminescence immunosensor built | |
| CN109444403B (en) | Ru (bpy) -based32+Preparation method of/Zn-oxalate immunosensor | |
| CN103134793B (en) | Electrogenerated chemiluminescence sensor with high sensitivity in cancer cell detection and fabrication method of sensor | |
| CN108802391B (en) | Based on TiO2Mesogen-induced resonance energy transfer type electrochemical luminescence and immunosensing method for ovarian cancer marker | |
| CN110794017B (en) | Preparation method of electrochemical immunosensor for detecting procalcitonin | |
| CN110346438B (en) | Based on PbS/Co3O4Preparation method of composite signal attenuation type photoelectrochemical immunosensor | |
| CN110261448A (en) | A kind of preparation method and application of the signal suppressive optical electro-chemistry Procalcitonin sensor based on zinc titanium composite material | |
| CN109283235A (en) | One kind being based on NSCQDs/Bi2S3Optical electro-chemistry sensor and its preparation and application | |
| CN108896638B (en) | Preparation method and application of immunosensor based on titanium dioxide doped graphene loaded sea cucumber-like gold-palladium core-shell nanoparticles | |
| CN106066324A (en) | A kind of preparation method and application of electroluminescent chemiluminescence biosensor label | |
| CN105044179B (en) | A kind of three-dimensional grapheme modified electrode detecting tumor markers and preparation method thereof | |
| CN110376380A (en) | A kind of application of electrochemistry Enzyme linked immunosensor and its preparation and detection antigen | |
| CN106226371A (en) | The preparation of cadmium sulfide cladding ceria hollow ball detection tumor cell sensor | |
| CN110530947A (en) | Based on WO3The building of the photoelectric sensor of/BiOI in conjunction with enzymatic precipitated phase | |
| CN103048314A (en) | Electrochemical luminescence immune sensor built by mesoporous material loading quantum dots and coated by nanogold, and detection method of HIV (human immunodeficiency virus) | |
| CN105675685B (en) | A kind of preparation method and application of the electrochemistry Spanon sensor based on additive Mn two-dimensional nano composite | |
| CN113155917B (en) | Construction method of photo-assisted bipolar self-powered sensor for detecting ochratoxin A or aflatoxin B1 | |
| CN113252747A (en) | Preparation method of self-powered sensor | |
| CN103454266A (en) | Method for preparing cadmium sulfide-coated zinc oxide nano array composite material and method for detecting multiple tumor cells | |
| CN105866221B (en) | The electrochemical sensor of reduced hemoglobin can be catalyzed | |
| CN104865240A (en) | Disposable nanometer electrogenerated chemiluminescence two-component immune sensor and preparation method thereof | |
| CN109490282B (en) | Based on NiFe2O4Nano-tube catalysis enhanced ovarian cancer marker ratio type electrochemiluminescence sensing platform | |
| CN111830101B (en) | Electrochemical luminescence sensor for detecting procalcitonin by doping ferrocenecarboxylic acid in ZIF-8 quenching RuSi nanoparticles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161214 |