CN106222241A - The screening technique of raw biocontrol bacterial strain in a kind of banana blight - Google Patents
The screening technique of raw biocontrol bacterial strain in a kind of banana blight Download PDFInfo
- Publication number
- CN106222241A CN106222241A CN201610652895.6A CN201610652895A CN106222241A CN 106222241 A CN106222241 A CN 106222241A CN 201610652895 A CN201610652895 A CN 201610652895A CN 106222241 A CN106222241 A CN 106222241A
- Authority
- CN
- China
- Prior art keywords
- bacterial strain
- banana
- culture
- agar
- described step
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention is the screening technique of raw biocontrol bacterial strain in providing a kind of banana blight, including following step: step A: the activation of bacterial strain and cultivation；Step B: the activation of pathogen and cultivation;Step C: endophytic bacterial controlled effect co-cultures with tissue culture seedlings of bananas；Step D: together with culture medium, the endogenetic fungus banana seedlings homobium obtained in step C is moved to length has on the water Agar Plating of Fusarium spp., cultivates in incubator.The invention has the beneficial effects as follows: the interior raw biocontrol bacterial strain indoor plate screening technique of resisting banana vascular wilt can effectively define the endogenetic fungal bacterial strain to banana blight with preferable biological and ecological methods to prevent plant disease, pests, and erosion function, provide theoretical basis for building banana blight biological prevention.
Description
Technical field
The invention belongs to biological pesticide technical field, be specifically related to the screening technique of a kind of banana blight biocontrol bacterial strain.
Background technology
Fructus Musae is the industrial crops that south China subtropical zone is important.But the scale continuous cropping plantation of Fructus Musae is led in recent years
Cause the multiple harm of silborne fungal diseases banana blight (Fusarium oxysporum.f sp.Cubense), morbidity
Banana garden diseased plant rate is generally up to 10%-40%, and serious reaches more than 80%, causes Jiao Yuan to leave uncultivated, and almost No kernels or seeds are gathered, as in a year of scarcity.At present,
For want of effective high-yield disease resisting kind, anti-Zhiduo County of banana blight relies on chemical prevention and cultural control is carried out, but the most not
Preferable prevention effect can be reached.Biological control is as one of the measure of integrated control banana blight, to ecological balance and can
The development of sustainable agriculture has the meaning of particular importance.
Plant endogenesis epiphyte is that a class can stablize the microorganism surely grown in host plant body, to host plant no pathogenicity
Resource (Jumppone, 2005；Knapp et al.,2015).Current reported research shows, some plant endogenesis epiphyte
Can not only promote the host plant absorption to mineral nutrition, improve the Biomass of seedling, have can also strengthen the anti-of plant
Inverse property, disease resistance etc..Wu&Guo (2008), with a plants endogenetic fungus inoculation Herba Saussureae Involueratae seedling, finds that inoculation can improve
The Biomass of seedling；Waller etc. (2005) and Baltmschat etc. (2008) test shows, endogenetic fungus
Piriformospora indica can induce Fructus Hordei Vulgaris to produce fungal disease (Fusarium spp.) and the system resistant of salt stress, strengthens
Fructus Hordei Vulgaris antioxidant system, improves glutathion and ascorbic acid content, increases yield.Narisawa etc. are from Fructus Solani melongenae, Fructus Melo, Fructus Hordei Vulgaris
With in the endogenetic fungus of strain more than 300 of isolated in the plant roots such as Chinese cabbage, filter out two strain biocontrol microorganisms Heteroconium
Chaetospira and Phialocephala fortinii, can significantly improve the resistance to verticillium wilt, wherein P.fortinii pair
The suppression ratio of celery cabbage clubroot has reached 97%, and pot experiment has also reached 67% to the suppression ratio of verticillium wilt.
Summary of the invention
The present invention can surely grow the feature forming mutualism body in host, relatively complete exposure according to endogenetic fungus
Other Biocontrol microorganism in rhizosphere soil is affected relatively small by environment, it is presumed that applied at disease biological
It is easier to during preventing and treating obtain stable prevention effect.Specifically, raw to separate to 8 strains of Caulis Sacchari sinensis rhizosphere and Rhizophora apiculata Blume plant
Fungal bacterial strain, as strains tested, filters out the endogenetic fungal bacterial strain with Biocontrol Potential, for by this bacterium by controlling experiment
Exploitation production is provided fundamental basis.
The screening technique of raw biocontrol bacterial strain in a kind of banana blight, including following step:
Step A: the activation of bacterial strain and cultivation: will activate on CMMY plating medium for examination endogenetic fungal bacterial strain, camera bellows
Middle cultivation, cuts the Endophytic Fungal Hyphae block activated and is inoculated in Herba bromi japonici plating medium；
Step B: the activation of pathogen and cultivation: former for banana blight bacterium-Fusarium spp. is activated in PDA plate culture medium
On, incubator is cultivated, cuts the Fusarium spp. inoculated by hypha block activated on water Agar Plating, standby；
Step C: endophytic bacterial controlled effect co-cultures with tissue culture seedlings of bananas: select the aseptic tissue culture seedlings of bananas that growing way is consistent to transplant extremely
On cultured endogenetic fungus bacterium colony, root system is laid in bacterium colony surface, co-cultures in incubator, it is thus achieved that endogenetic fungus Fructus Musae
Seedling homobium；
Step D: together with culture medium, the endogenetic fungus banana seedlings homobium obtained in step C is moved to length sickle
On the water Agar Plating of cutter bacterium, cultivate in incubator.
Preferably, in described step C, with plantation process on the blank oat medium not inoculating endogenetic fungal bacterial strain
For comparison；In described step D, it is directly accessed on the water Agar Plating with Fusarium spp. with comparison in described step C
It is processed as comparison.
Preferably, the sick level situation that observed and recorded respectively processes, and calculate disease index and prevention effect:
Preferably, in described step A, condition of culture is: light culture 10d in 28 DEG C of incubators.
Preferably, the formula of described CMMY plating medium is: wort agar 10g/L, corn meal agar 8.5g/L, ferment
Female cream 2g/L, agar 7.5g/L.
Preferably, the formula of described Herba bromi japonici plating medium is: oatmeal 10g/L, agar 18g/L, MgSO4·7H2O
1g/L, KH2PO41.5g/L, NaNO3 1g/L。
Preferably, in described step B, the formula of described PDA plate culture medium is: in described step B, described PDA plate
The formula of culture medium is: Rhizoma Solani tuber osi 200g/L, glucose 15g/L；Agar 15g/L；Wherein, the preparation method of the solution of Rhizoma Solani tuber osi is will
Rhizoma Solani tuber osi boils 30mins in water, filters the filtrate obtained after 4 times.
Preferably, in described step B, condition of culture is: temperature is 28 DEG C, time 7d.
Preferably, in described step C, condition of culture is: temperature 25 DEG C, intensity of illumination 180 μm ol/m2·s2, light application time
The incubator of 16h/d co-cultures 15d.
Preferably, in described step D, condition of culture is: temperature 28 DEG C, intensity of illumination 180 μm ol/m2·s2, humidity
80%, incubation time 15d.
The invention has the beneficial effects as follows: the interior raw biocontrol bacterial strain indoor plate screening technique of resisting banana vascular wilt can effective boundary
Make the endogenetic fungal bacterial strain that banana blight is had preferable biological and ecological methods to prevent plant disease, pests, and erosion function, carry for building banana blight biological prevention
Supply theoretical basis.
Accompanying drawing explanation
The sick level grade scale of Fig. 1 endogenetic fungal bacterial strain biological and ecological methods to prevent plant disease, pests, and erosion indoor plate screening.
Endophytic Fungal Hyphae in Fig. 2 banana seedlings root tissue.
Fig. 3 difference endogenetic fungal bacterial strain indoor plate biocontrol effect data to banana blight.
Fig. 4 endogenetic fungal bacterial strain L-14 plate prevention effect to banana blight.
Detailed description of the invention
Below in conjunction with the accompanying drawings, the preferably embodiment of the present invention is described in further detail:
Embodiment 1
The activation of step 1. bacterial strain will activate in CMMY plating medium (beerwort fine jade for examination endogenetic fungal bacterial strain with cultivating
Fat 10g/L, corn meal agar 8.5g/L, yeast extract 2g/L, agar 7.5g/L) on, light culture 10d in 28 DEG C of incubators.Cut
The Endophytic Fungal Hyphae block activated is inoculated in Herba bromi japonici plating medium (oatmeal 10g/L, agar 18g/L, MgSO4·7H2O
1g/L, KH2PO41.5g/L, NANO3On 1g/L), every ware 3 truffle of inoculation, cultivate 10d, standby.
Former for banana blight bacterium-Fusarium spp. is activated in PDA plate culture medium (soil by the activation of step 2. pathogen with cultivating
Bean 200g/L, boils 30mins, filters and takes filtrate 4 times；Glucose 15g/L；Agar 15g/L) on, light culture in 28 DEG C of incubators
7d.Cut the Fusarium spp. inoculated by hypha block activated on water Agar Plating (agar 9g/L), every ware 3 bacterium of inoculation
Block, cultivates 10d, standby.
Step 3. endophytic bacterial controlled effect selects the aseptic tissue culture seedlings of bananas that growing way is consistent to transplant extremely with co-culturing of tissue culture seedlings of bananas
On cultured endogenetic fungus bacterium colony, root system is laid in bacterium colony surface, and every bacterium colony transplants a strain tissue cultured seedling, and 3 strains planted by every ware.Will training
Foster ware is put in tissue culture bottle, in 25 DEG C, intensity of illumination 180 μm ol/m2·s2, light application time 16h/d incubator in co-culture
15d, it is thus achieved that endogenetic fungus banana seedlings homobium.With plantation on the blank oat medium not inoculating endogenetic fungal bacterial strain
It is processed as comparison, often processes 3 repetitions, often repeat 7 wares.
The endogenetic fungus banana seedlings that the lab screening of step 4. banana blight biological and ecological methods to prevent plant disease, pests, and erosion endophytic bacterial controlled effect will obtain in step 3
Homobium moves to length together with culture medium to be had on the water Agar Plating of Fusarium spp., is placed in 28 DEG C, intensity of illumination 180
μmol/m2·s2, the constant temperature and humidity incubator of humidity 80% is cultivated 15d.Comparison in step 3 is then directly accessed with sickle
On the water Agar Plating of cutter bacterium.The sick level situation that observed and recorded respectively processes, and calculate disease index and prevention effect.
Indoor plate screening test severity Scaling standard: 0 grade-outward appearance is without Visual symptoms；1 grade-banana plant growing way is without bright
Aobvious being obstructed, the Herb Huang area that withers does not surpasses 35%；2 grades-banana plant is small and weak, and the Herb Huang area that withers reaches 35%-80%；3 grades-Fructus Musae
The plant Herb Huang area that withers reaches more than 80%, even Herb withered (as shown in Figure 1).Its formula is:
Embodiment 2 endogenetic fungus banana seedlings homobium is set up
Test result indicate that, endogenetic fungal bacterial strain can be stablized and surely grows in host plant body, no pathogenicity and planting with host
After thing forms this saying endogenetic fungus banana seedlings homobium foundation of mutualism body, banana seedlings plant remains to healthy one-tenth
Long, and find after root tissue microscopy, with the presence of endophytic fungal hypha (as shown in Figure 2) in root tissue.
The interior raw biocontrol bacterial strain indoor plate screening of embodiment 3 resisting banana vascular wilt
Material to be tested
For examination tissue culture seedlings of bananas: the osmanthus any of several broadleaf plants No. 6 provided for GUIHAIA tissue cultured seedling company limited.
For examination endogenetic fungal bacterial strain: 8 strain endogenetic fungal bacterial strains are by separating acquisition, experiment in Caulis Sacchari sinensis rhizosphere and Rhizophora apiculata Blume plant
Room preserves.
For examination pathogen: Pathogen Causing Banana Fusarium Wilt reaping hook germina number-four biological strain, it is isolatable from Hainan banana blight sick
Strain, laboratory preserves.
Shown in the inventive method, laboratory is separated the endogenetic fungal bacterial strain obtained and carries out biological and ecological methods to prevent plant disease, pests, and erosion functional screening.Knot
Fruit shows, shown in Fig. 3 and Fig. 4, the biocontrol effect power that banana blight is shown by different endogenetic fungal bacterial strains differs, bacterial strain
The indoor plate biocontrol effect of L-14 is best, and up to 72.42%, and the indoor plate biocontrol effect of bacterial strain HS6-F-9 is only
9.09%.This also illustrates, the interior raw biocontrol bacterial strain indoor plate screening technique of resisting banana vascular wilt can effectively define perfume (or spice)
Any of several broadleaf plants droop has the endogenetic fungal bacterial strain of preferable biological and ecological methods to prevent plant disease, pests, and erosion function.
Above content is to combine concrete preferred implementation further description made for the present invention, it is impossible to assert
Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of present inventive concept, it is also possible to make some simple deduction or replace, all should be considered as belonging to the present invention's
Protection domain.
Claims (10)
1. the screening technique of raw biocontrol bacterial strain in a banana blight, it is characterised in that include following step:
Step A: the activation of bacterial strain and cultivation: will activate on CMMY plating medium for examination endogenetic fungal bacterial strain, and train in camera bellows
Support, cut the Endophytic Fungal Hyphae block activated and be inoculated in Herba bromi japonici plating medium；
Step B: the activation of pathogen and cultivation: former for banana blight bacterium-Fusarium spp. is activated in PDA plate culture medium, training
Support in case and cultivate, cut the Fusarium spp. inoculated by hypha block activated on water Agar Plating, standby；
Step C: endophytic bacterial controlled effect co-cultures with tissue culture seedlings of bananas: select the aseptic tissue culture seedlings of bananas that growing way is consistent to transplant to cultivating
On good endogenetic fungus bacterium colony, root system is laid in bacterium colony surface, co-cultures in incubator, it is thus achieved that endogenetic fungus banana seedlings is altogether
Raw body；
Step D: together with culture medium, the endogenetic fungus banana seedlings homobium obtained in step C is moved to length Fusarium spp.
Water Agar Plating on, in incubator cultivate.
2. the method for claim 1, it is characterised in that in described step C, is not inoculating endogenetic fungal bacterial strain with plantation
Blank oat medium on process for comparison；In described step D, it is directly accessed with reaping hook with comparison in described step C
Process on the water Agar Plating of bacterium is comparison.
3. method as claimed in claim 2, it is characterised in that also include: the sick level situation that observed and recorded respectively processes, and calculate
Disease index and prevention effect, its formula is:
4. the method for claim 1, it is characterised in that in described step A, condition of culture is: dark in 28 DEG C of incubators
Cultivate 10d.
5. the method for claim 1, it is characterised in that the formula of described CMMY plating medium is: wort agar
10g/L, corn meal agar 8.5g/L, yeast extract 2g/L, agar 7.5g/L.
6. the method for claim 1, it is characterised in that the formula of described Herba bromi japonici plating medium is: oatmeal 10g/
L, agar 18g/L, MgSO4·7H2O 1g/L, KH2PO41.5g/L, NANO3 1g/L。
7. the method for claim 1, it is characterised in that in described step B, the formula of described PDA plate culture medium is:
Rhizoma Solani tuber osi 200g/L, glucose 15g/L；Agar 15g/L；Wherein, the preparation method of the solution of Rhizoma Solani tuber osi is for boil Rhizoma Solani tuber osi in water
30mins, filters the filtrate obtained after 4 times.
8. the method for claim 1, it is characterised in that in described step B, condition of culture is: temperature is 28 DEG C, the time
7d。
9. the method for claim 1, it is characterised in that in described step C, condition of culture is: temperature 25 DEG C, illumination are strong
Spend 180 μm ol/m2·s2, light application time 16h/d incubator in co-culture 15d.
10. the method for claim 1, it is characterised in that in described step D, condition of culture is: temperature 28 DEG C, illumination
Intensity 180 μm ol/m2·s2, humidity 80%, incubation time 15d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610652895.6A CN106222241A (en) | 2016-08-10 | 2016-08-10 | The screening technique of raw biocontrol bacterial strain in a kind of banana blight |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610652895.6A CN106222241A (en) | 2016-08-10 | 2016-08-10 | The screening technique of raw biocontrol bacterial strain in a kind of banana blight |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106222241A true CN106222241A (en) | 2016-12-14 |
Family
ID=57548439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610652895.6A Pending CN106222241A (en) | 2016-08-10 | 2016-08-10 | The screening technique of raw biocontrol bacterial strain in a kind of banana blight |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106222241A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102935A (en) * | 2018-01-10 | 2018-06-01 | 郑州轻工业学院 | One plant of cucumber, the screening technique of sponge gourd droop antagonistic yeast and application |
| CN110804556A (en) * | 2019-11-28 | 2020-02-18 | 广西壮族自治区农业科学院微生物研究所 | Endophytic fungus LZ3 and application thereof |
| CN111944668A (en) * | 2020-08-26 | 2020-11-17 | 慕恩（广州）生物科技有限公司 | Device for screening microorganisms, kit thereof and method thereof |
| CN112195109A (en) * | 2020-11-09 | 2021-01-08 | 广西壮族自治区农业科学院微生物研究所 | Endophytic fungi and application thereof |
| CN112280692A (en) * | 2020-11-09 | 2021-01-29 | 广西壮族自治区农业科学院微生物研究所 | Crop growth-promoting disease-resistant DSE strain and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981101A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark Septate Endophyte) strain and application of DSE strain in production of sugarcane |
-
2016
- 2016-08-10 CN CN201610652895.6A patent/CN106222241A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103981101A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark Septate Endophyte) strain and application of DSE strain in production of sugarcane |
Non-Patent Citations (3)
| Title |
|---|
| 余超等: "生防菌FJAT-346-PA-K的内生定殖特性及对香蕉枯萎病的防治效果", 《植物保护学报》 * |
| 甘林等: "枯草芽孢杆菌T122F菌剂对香蕉的生物效应研究", 《热带作物学报》 * |
| 白丹虹: "香蕉枯萎病生防菌的筛选、防治作用及生防机制初探", 《中国优秀硕士学位论文全文数据库农业科技辑（电子期刊）》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108102935A (en) * | 2018-01-10 | 2018-06-01 | 郑州轻工业学院 | One plant of cucumber, the screening technique of sponge gourd droop antagonistic yeast and application |
| CN110804556A (en) * | 2019-11-28 | 2020-02-18 | 广西壮族自治区农业科学院微生物研究所 | Endophytic fungus LZ3 and application thereof |
| CN111944668A (en) * | 2020-08-26 | 2020-11-17 | 慕恩（广州）生物科技有限公司 | Device for screening microorganisms, kit thereof and method thereof |
| CN111944668B (en) * | 2020-08-26 | 2022-09-13 | 慕恩（广州）生物科技有限公司 | Device for screening microorganisms, kit thereof and method thereof |
| CN112195109A (en) * | 2020-11-09 | 2021-01-08 | 广西壮族自治区农业科学院微生物研究所 | Endophytic fungi and application thereof |
| CN112280692A (en) * | 2020-11-09 | 2021-01-29 | 广西壮族自治区农业科学院微生物研究所 | Crop growth-promoting disease-resistant DSE strain and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100500005C (en) | Preparation method for preventing and treating vegetable fungicide microbial pesticides | |
| CN106222241A (en) | The screening technique of raw biocontrol bacterial strain in a kind of banana blight | |
| CN106497831B (en) | A kind of prevention and treatment Phytophthora nicotianae disease composite bacteria agent and its preparation method and application | |
| CN103103152B (en) | Corallococcus coralloides and application thereof | |
| CN105543132A (en) | Bacillus methylotrophicus YB-F7 and application thereof in preventing plant diseases | |
| CN110423707A (en) | Bacillus subtilis strain NH-6 and its application | |
| CN1994092A (en) | A biological weed killer and preparation process thereof | |
| CN103053624B (en) | Method for control of phytophthora blight of pepper by mixed application of trichoderma preparation and fungicides | |
| CN103160442A (en) | Paecilomyceslilacinus strain having strong pathogenicity for diaphorina citri | |
| CN102154157B (en) | Bio-control strain 4AT8 capable of preventing and curing various bacterial diseases | |
| CN105886405A (en) | Dendrobium officinale Kimura et Migo endophytic fungus and applications thereof | |
| CN106676049A (en) | Bacillus amyloliquefaciens strain and application thereof | |
| CN102586120B (en) | Endophytic fungi CEF08111 of cotton and application thereof in prevention and treatment of cotton verticillium wilt | |
| CN106085885A (en) | The Trichoderma asperellum of a kind of antagonism banana blight and application thereof | |
| CN105462881A (en) | Paenibacillus polymyxa for preventing and treating vertieillium wilt in crops and application of paenibacillus polymyxa | |
| CN103087947B (en) | Pseudoxanthomonas sp. ZKB4-4 and application thereof | |
| CN104789509B (en) | Raw bacillus pumilus and its application in one plant of bark of eucommia | |
| CN105557347A (en) | Gray mold resistance identified seedling stage inoculation method for capsicum | |
| CN102108339A (en) | Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof | |
| CN108384734A (en) | A kind of biocontrol agent and preparation method thereof of prevention tobacco rhizome class disease | |
| CN103642704B (en) | Cotton endogenetic fungus CEF-714 and the application in cotton verticillium wilt control thereof | |
| CN106222121B (en) | A kind of bacillus megaterium bacterial strain, biocontrol agent and the preparation method and application thereof | |
| CN105039414A (en) | Penicillium fermentation liquid for preventing and treating tobacco black shank and preparation method thereof | |
| CN111733081A (en) | Biological agent formula suitable for preventing and treating tomato root-knot nematode diseases | |
| CN103146609B (en) | Pseudomonas fluorescens and method for preventing phytophthora capsici thereby |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161214 |