CN106199198B - Simple in-vitro skin percutaneous resistance measuring device - Google Patents
Simple in-vitro skin percutaneous resistance measuring device Download PDFInfo
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- CN106199198B CN106199198B CN201610827046.XA CN201610827046A CN106199198B CN 106199198 B CN106199198 B CN 106199198B CN 201610827046 A CN201610827046 A CN 201610827046A CN 106199198 B CN106199198 B CN 106199198B
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- 238000000338 in vitro Methods 0.000 title claims abstract description 44
- 210000005056 cell body Anatomy 0.000 claims abstract description 101
- 210000004027 cell Anatomy 0.000 claims abstract description 76
- 241001465754 Metazoa Species 0.000 claims abstract description 53
- 239000003792 electrolyte Substances 0.000 claims abstract description 30
- 238000005259 measurement Methods 0.000 claims abstract description 18
- 238000001514 detection method Methods 0.000 claims abstract description 6
- 238000005260 corrosion Methods 0.000 claims description 13
- 230000007797 corrosion Effects 0.000 claims description 12
- 238000007599 discharging Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 239000011810 insulating material Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 3
- 239000007769 metal material Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 6
- 239000003814 drug Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 206010040880 Skin irritation Diseases 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 231100000108 skin corrosion Toxicity 0.000 description 3
- 231100000475 skin irritation Toxicity 0.000 description 3
- 230000036556 skin irritation Effects 0.000 description 3
- 238000005452 bending Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 241001661918 Bartonia Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 230000007799 dermal corrosion Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R27/00—Arrangements for measuring resistance, reactance, impedance, or electric characteristics derived therefrom
- G01R27/02—Measuring real or complex resistance, reactance, impedance, or other two-pole characteristics derived therefrom, e.g. time constant
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Abstract
The invention provides a simple in-vitro skin percutaneous resistance measuring device, which comprises a linkage clamping plate with a fastening screw, wherein a pair of upper electrolytic cell and lower electrolytic cell which can inject or discharge upper electrolyte and lower electrolyte are arranged between an upper clamping plate opening and a lower clamping plate opening of the linkage clamping plate, the upper electrolytic cell is provided with an upper cell body opening with a downward opening, the lower electrolytic cell is provided with a lower cell body opening with an upward opening, in-vitro animal skin can be sealed and fixed between the upper cell body opening and the lower cell body opening, an upper electrode and an electrode interface are installed and fixed on the side wall of the upper electrolytic cell, a lower electrode and an electrode interface are also installed and fixed on the side wall of the lower electrolytic cell, and an outer electrode wire output by a resistance measuring instrument is connected to the two electrode interfaces to complete the measurement of the in-vitro animal skin percutaneous resistance, and the invention has the beneficial effects that: the measurement device for the in vitro animal skin percutaneous resistance is developed, which is simple, convenient and quick to operate, and solves the problem of carrying out in vitro animal skin percutaneous resistance detection simply, quickly, conveniently and standardly.
Description
Technical Field
The invention relates to a simple in-vitro skin percutaneous resistance measuring device.
Background
Corresponding experiments are often required to be carried out in recent identification and scientific research on skin irritation and corrosivity, most of the experiments in the past are carried out by using living animals, the experiments of the living animals are less and less used at present, more attention is paid to the fact that the skin irritation, corrosivity and other alternative method experiments are carried out by using the skin of an in-vitro animal, the skin of the in-vitro animal is fixed, and the skin resistance value is measured to distinguish the irritation and the corrosivity of the skin; at present, a plurality of methods are adopted for measuring the skin percutaneous resistance of the in-vitro animal, some methods adopt high-end equipment for measurement, but the operation is complex, the price is high, and some experimental researches cannot bear; in order to overcome the difficulties, the difficult problems of rapid, accurate, standard, batch, simple and convenient operation and low price in the percutaneous resistance detection work of the in vitro animal skin are fundamentally solved, and the research and development of a novel full-simple in vitro animal skin percutaneous resistance measuring device which does not use the traditional method to measure the percutaneous resistance of the in vitro animal skin is inevitable and urgent.
Disclosure of Invention
In order to solve the problems, the invention aims to provide a simple in-vitro skin percutaneous resistance measuring device, which adopts a simple linkage clamping plate similar to a movable clamp, fixes in-vitro animal skin between an upper electrolytic cell and a lower electrolytic cell which are arranged on the linkage clamping plate, and is directly connected with a resistance measuring instrument to measure the percutaneous resistance, thereby solving the difficult problem of simply, quickly, conveniently and normally carrying out the in-vitro animal skin percutaneous resistance detection work.
The invention provides a simple in-vitro skin percutaneous resistance measuring device, which comprises a linkage clamping plate which is similar to a movable clamp and is provided with a fastening screw rod capable of controlling the tightness degree, an upper electrolytic cell and a lower electrolytic cell are fixedly arranged between an upper clamping plate port and a lower clamping plate port which are provided with notches at the front end of the linkage clamping plate, the uppermost edge of the upper electrolytic cell is an upper cell cylinder port used for injecting or discharging electrolyte at any time, the lowermost edge of the upper electrolytic cell is an upper cell body port with an opening smooth to the lower port edge, an upper electrode and an electrode interface used for connecting an external electrode wire output by a resistance measuring instrument are fixedly arranged on the side wall of the upper cell body at a certain distance away from the upper cell body port in parallel to the upper cell body port for percutaneous resistance measurement, the uppermost edge of the lower electrolytic cell is a lower cell body port with an opening smooth to the upper port edge, the in-vitro animal skin is hermetically fixed between the upper cell body port and the lower cell body port, the small opening at the lower cell body port is communicated with a section of a cylindrical extension pipe with a bending return pipe, the lower cylinder port with which is an upper opening upward, the lower cell body port, the extension pipe is used for injecting or discharging the lower cell body port, the outer electrode at any time, the lower cell body port is connected with the lower cell, a certain distance away from the upper cell body port, and the lower cell body, the lower cell electrode is connected with the lower cell electrode, and the lower cell for percutaneous resistance measuring instrument for percutaneous electrode, and the lower electrode are directly connected with the lower electrode, and the lower electrode.
The invention has the beneficial effects that: the simple in-vitro skin percutaneous resistance measuring device is developed, the problems of inaccurate measurement, low single measuring speed and operator pollution caused by a traditional measuring method of manual operation are solved, the in-vitro animal skin percutaneous resistance can be simply, conveniently and quickly measured without purchasing expensive special equipment, and the problem of simply, quickly, conveniently, economically and standardly carrying out in-vitro animal skin percutaneous resistance detection work in scientific research and experiments is fundamentally solved. The method is widely applied to skin irritation and corrosion experiments.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention.
In the figure:
1. an upper electrode 2, an upper cell body 3, an upper clamp bracket 4, an upper clamp internal thread 5, an upper clamp plate handle 6, a lower cell barrel opening 7 and lower electrolyte
8. An upper screw 9, a screw rotating handle 10, a fastening screw 11, a lower screw 12, a lower clamp internal thread 13 and a lower clamp plate handle
14. Linkage splint 15, lower electrolytic cell 16, support spring 17, support shaft 18, lower splint support 19 and lower cell body opening
20. Extension tube 21, lower clamp plate 22, lower tank body 23, lower electrode 24, inner electrode seat 25, electrode interface 26, electrode seat hole
27. External electrode plug 28, lower splint opening 29, external electrode wire 30, in-vitro animal skin 31, upper cell body opening 32 and upper splint
33. Operation panel 34, resistance measuring instrument 35, display 36, printer 37, upper clamping plate port 38, upper electrolyte
39. And (4) feeding the electrolytic cell through a cell barrel opening 40.
Detailed Description
The invention is described with reference to the accompanying drawings and examples.
As shown in the attached drawings, the invention provides a simple in-vitro skin percutaneous electrical resistance measuring device, which is characterized in that: the movable clamp-like linkage clamping plate 14 comprises a movable clamp-like linkage clamping plate 14 with a fastening screw rod 10 capable of controlling tightness degree, the linkage clamping plate 14 is made of rigid corrosion-resistant materials, a mechanical device for clamping and fixing the in-vitro animal skin 30 between an upper electrolytic cell 40 and a lower electrolytic cell 15 is provided, the upper electrolytic cell 40 and the lower electrolytic cell 15 are fixedly arranged between an upper clamping plate opening 37 and a lower clamping plate opening 28 which are provided with notches at the front end of the linkage clamping plate 14, the front parts of a pair of upper clamping plate 32 and a lower clamping plate 21 which are in the same shape and opposite direction are respectively provided with an upper clamping plate opening 37 and a lower clamping plate opening 28 which are opened forwards, the shapes and the sizes of the upper clamping plate opening 37 and the lower clamping plate opening 28 can just accommodate the edges of an upper cell body 2 of the upper electrolytic cell 40 and a lower cell body 22 of the lower electrolytic cell 15, the middle parts of the upper clamping plate 32 and the lower clamping plate 21 are provided with upper clamping bracket 3 and a lower clamping bracket 18 which are in the same shape and opposite direction and are respectively movably connected together through a bracket shaft 17, the bracket shaft 17 is sleeved with a bracket spring 16, the upper splint handle 5 and the lower splint handle 13 are supported and fixed in the separation direction in the upper splint handle 5 and the lower splint handle 13, the respective rear parts of the upper splint 32 and the lower splint 21 are provided with the upper splint handle 5 and the lower splint handle 13 which have the same shape and are opposite in direction, the upper splint handle 5 and the lower splint handle 13 are respectively provided with an upper splint internal thread 4 and a lower splint internal thread 12 which have the same shape and are opposite in direction, a fastening screw 10 is meshed between the upper splint internal thread 4 and the lower splint internal thread 12, the distance between the upper splint handle 5 and the lower splint handle 13 can be adjusted at any time, and the upper splint support 3 and the lower splint support 18 are movably linked together through the bracket shaft 17, so that the separated animal skin between the upper electrolytic cell 40 and the lower electrolytic cell 15 can be pressed or loosened by changing the distance between the upper splint opening 37 and the lower splint opening 28 under the lever principle Purpose of 30; the fastening screw 10 is a fastening element which is vertically arranged at the rear part of the linkage clamping plate 14 and is made of firm corrosion-resistant materials, the middle part of the fastening screw 10 is a disk-shaped screw rotating handle which is rotated by hands, the center of the disk extends out of an upper screw 8 and a lower screw 11 from the upper end to the lower end respectively, the thread direction of the upper screw 8 is opposite to the thread direction of the lower screw 11, the thread of the upper screw 8 is meshed in the upper clamp internal thread 4 at the rear part of the linkage clamping plate 14, the thread of the lower screw 11 is meshed in the lower clamp internal thread 12 at the rear part of the linkage clamping plate 14, when an operator rotates the screw rotating handle 9 of the fastening screw 10 in one direction by hands, the upper screw 8 of the fastening screw 10 rotates, the upper clamping plate handle 5 is forced to one direction by the meshing force with the upper clamping internal thread 4, meanwhile, the lower screw 11 of the fastening screw 10 is forced to the lower clamping plate handle 13 by the meshing force with the lower clamping internal thread 12 to the other opposite direction, so as to form the expansion or clamping action of the upper clamping plate handle 5 and the lower clamping plate handle 13, and as the upper clamping bracket 3 and the lower clamping bracket 18 are constrained in the bracket shaft 17 to form a lever principle, the upper clamping plate 32 and the lower clamping plate 21 are clamped or expanded at the same time of the expansion or clamping action of the upper clamping plate handle 5 and the lower clamping plate handle 13, and the upper electrolytic cell 40 and the lower electrolytic cell 15 which are placed in the upper clamping plate opening 37 and the lower clamping plate opening 28 are clamped or loosened, so that the air tightness of experimental detection and the convenience of disassembly are ensured; the upper electrolytic cell 40 is a cavity of an inverted cone-shaped funnel with smooth inner and outer walls made of firm and corrosion-resistant insulating materials, the uppermost edge of the cavity of the inverted cone-shaped funnel is a cylindrical upper cell tube opening 39 with an upward opening, electrolyte 38 can be injected or discharged from the opening at any time, a cylinder below the upper cell tube opening 39 is communicated with the upper edge of an upper cell body 2 of the inverted cone-shaped funnel in an extending manner, the large opening edge of the inverted cone-shaped funnel at the bottommost edge of the upper cell body 2 is an upper cell body opening 31 with a smooth and flat opening, the opening is downward, the diameter and the shape of the upper cell body opening 31 are completely the same as those of a lower cell body opening 19 of a lower electrolytic cell 15 arranged at the lower edge of a corresponding position, the upper cell body opening 19 is aligned and matched to compress an isolated animal skin 30, and the sealing is ensured that the upper electrolyte 38 cannot leak when the upper electrolytic cell 40 is injected, an upper electrode 1 and an electrode interface 25 are fixedly arranged on the side wall of the upper cell body 2 at a certain distance away from the upper cell body opening 31 and are parallel to the upper cell body opening 31, and are used for connecting with an external electrode wire 29 output by a resistance measuring instrument 34 to measure resistance through skin resistance; the lower electrolytic cell 15 is a cavity of a conical funnel with smooth inner and outer walls and an upward large opening and a prolonged tube 20 made of a firm corrosion-resistant insulating material, the conical funnel part in the cavity of the conical funnel with the upward large opening is a lower cell body 22, a small opening edge at the lower part of the lower cell body 22 is extended and communicated to form a section of cylindrical prolonged tube 20 with a bending and turning back, the other end of the prolonged tube 20 is a lower cell body opening 6 with an upward opening, lower electrolyte 7 can be injected or discharged from the lower cell body opening at any time, a large opening edge of the conical funnel at the upper part of the lower cell body 22 is a lower cell body opening 19 with an upward opening and smooth and flat edge, the diameter and shape of the lower cell body opening 19 are completely the same as those of an upper cell body opening 31 of an upper electrolytic cell 40 arranged at the upper edge of a corresponding position and are aligned, pressed and sealed and fixed with an in-vitro animal skin 30, the sealing condition that no leakage exists when the lower electrolyte 7 is injected into the lower electrolytic cell body 15 is ensured, a lower electrolytic cell body 22 is parallel to the lower cell body opening 19, a lower electrode 23 and an electrode interface 25 are arranged on the side wall of the lower cell body 22 at a certain distance from the lower cell body opening 19, and fixed, and used for connecting with an external electrode wire 29 for direct resistance measurement to finish percutaneous resistance measurement of an in-skin measurement; the upper electrode 1 and the lower electrode 23 are both dense conductive nets made of metal materials with good conductivity, different conductive areas and diameters are selected according to the isolated animal skin 30 with different areas and diameters and corresponding requirements, the outer surfaces of the upper electrode 1 and the lower electrode 23 are smooth and straight so as to be conductive and prevent pollution and corrosion, the upper electrode 1 and the lower electrode 23 are respectively and parallelly installed on the upper tank body 2 and the lower tank body 22 with equal distance to the isolated animal skin 30, the electrode interfaces 25 are installed on the edges of the upper electrode 1 and the lower electrode 23 respectively, and are connected with the resistance measuring instrument 34 through corresponding external electrode wires 29, the resistance measuring instrument 34 accurately measures the percutaneous resistance value of the isolated animal skin 30 clamped and fixed between the upper electrolytic tank 40 and the lower electrolytic tank 15 through the external electrode wires 29 and the electrode interfaces 25 and the upper electrode 1 and the lower electrode 23 respectively installed in the upper electrolytic tank 40 and the lower electrolytic tank 15, the percutaneous resistance value is stored through a storage circuit in the machine or output to the display screen 35 and the printer 36, and data can be called or printed through the operation of the operation panel 33; the electrode interfaces 25 are a pair of electronic components made of a good conductive and corrosion resistant material and fixedly installed on the edges of the upper tank body 2 and the lower tank body 22 respectively, each electrode interface 25 is provided with an inner electrode seat 24 which penetrates through the edges of the upper tank body 2 and the lower tank body 22 and is sealed and installed without any leakage or looseness, the inner electrode seats 24 are immersed in the upper electrolyte 38 and the lower electrolyte 7 filled in the upper tank body 2 and the lower tank body 22 and are fixedly connected with the upper electrode 1 and the lower electrode 23 to form conduction current through in-vitro animal skin, each electrode interface 25 is also provided with a pair of electrode seat holes 26 and outer electrode plugs 27 which can be inserted and disconnected at any time, the electrode seat holes 26 are communicated with the upper electrode 1 and the lower electrode 23 through the inner electrode seats 24, the outer electrode plugs 27 are connected with a resistance measuring instrument 34 through outer electrode wires 29, and when the outer electrode plugs 27 connected with the outer electrode wires 29 of the resistance measuring instrument 34 are inserted into the electrode seat holes 26, the upper electrode 1 and the lower electrode 23 communicated with the inner electrode seats 24 form percutaneous current measurement through the in-vitro animal skin 30 filled with the upper electrolyte 38 and the lower tank body 2 and the lower electrolyte 7; the resistance measuring instrument 34 is an electronic instrument which can measure the output voltage and current of the in-vitro animal skin 30 fixed on the linkage splint 14 through the external electrode wire 29 and then measure the percutaneous resistance of the in-vitro animal skin 30 according to ohm's law, the resistance measuring instrument 34 is internally provided with an electronic instrument consisting of an intelligent computer control program circuit and a precise resistance measuring circuit, the precise resistance measuring circuit arranged in the electronic instrument is connected to the external electrode wire 29 to precisely measure the resistance value of the in-vitro animal skin 30 fixed on the linkage splint 14, the upper part of the resistance measuring instrument 34 is provided with a display screen 35 which can display the measured percutaneous resistance value and a set program at any time, the front surface of the resistance measuring instrument 34 is provided with an operation panel 33 which can conveniently set a measuring program and requirements, and the top of the resistance measuring instrument 34 is provided with a printer 36 which can print the measured percutaneous resistance value and the set program at any time.
Example (b):
when in use, firstly, a plurality of pieces of fresh in-vitro animal skin 30 which is coated with tested drugs or is treated and tested for experiment are prepared according to needs, and proper upper electrolyte 38 and lower electrolyte 7 with certain concentration and requirements are prepared; printing paper with the printer 36 mounted on top of the resistance measuring instrument 34 ready for printing; adjusting a disk-shaped screw handle of a fastening screw 10 vertically installed at the rear of a linkage clamping plate 14, when an operator rotates a screw handle 9 of the fastening screw 10 in one direction by hand, an upper screw 8 of the fastening screw 10 rotates to apply force to an upper clamping plate handle 5 in one direction by engaging with an upper clamping internal thread 4, and at the same time, a lower screw 11 of the fastening screw 10 applies force to a lower clamping plate handle 13 in the other opposite direction by engaging with a lower clamping internal thread 12 to form a compression action of the upper clamping plate handle 5 and the lower clamping plate handle 13, because the upper clamping bracket 3 and the lower clamping bracket 18 are constrained in a bracket shaft 17 to form a lever principle, an expansion action of an upper clamping plate 32 and a lower clamping plate 21 is caused while the upper clamping plate handle 5 and the lower clamping plate handle 13 are compressed, and an upper electrolytic cell 40 and a lower electrolytic cell 15 placed in an upper clamping plate opening 37 and a lower clamping plate opening 28 are loosened, at this time, a prepared experimental fresh animal skin 30 which has been coated with a test substance or has been treated and tested is laid flat on a lower cell body opening 19 of the lower electrolytic cell 15; then the operator rotates the screw stem handle 9 of the fastening screw 10 in the opposite direction by hand again, the upper screw 8 of the fastening screw 10 rotates, and applies force to the upper clamping plate handle 5 in one direction by engaging force with the upper clamp internal thread 4, and at the same time, the lower screw 11 of the fastening screw 10 applies force to the lower clamping plate handle 13 in the other opposite direction by engaging force with the lower clamp internal thread 12, so as to form an expanding action of the upper clamping plate handle 5 and the lower clamping plate handle 13, and since the upper clamping bracket 3 and the lower clamping bracket 18 are constrained in the bracket shaft 17 to form a lever principle, clamping action is caused to the upper clamping plate 32 and the lower clamping plate 21 while the upper clamping plate handle 5 and the lower clamping plate handle 13 are expanded, and the upper electrolytic cell 40 and the lower electrolytic cell 15 placed in the upper clamping plate opening 37 and the lower clamping plate opening 28 are clamped; thus, the lower cell body port 19 of the lower electrolytic cell 15 and the upper cell body port 31 of the upper electrolytic cell 40 arranged at the upper side of the corresponding position are aligned, matched, pressed, sealed and fixed with the in vitro animal skin 30; injecting an upper electrolyte 38 into the upper cell body 2 from an upper cell cylindrical opening 39 of an upper electrolytic cell 40, and injecting a lower electrolyte 7 into the lower cell body 22 from a lower cell cylindrical opening 6 through an extension pipe 20 of a lower electrolytic cell 15; turning on a power switch of a resistance measuring instrument 34, setting a measuring program according to experimental requirements through a front operation panel 33, displaying the set program through a display screen 35, inserting an outer electrode plug 27 connected with an outer electrode wire 29 of the resistance measuring instrument 34 into an electrode holder hole 26 in an electrode interface 25 of an upper electrode 1 and a lower electrode 23, starting a click experiment, communicating the electrode holder hole 26 with the upper electrode 1 and the lower electrode 23 through an inner electrode holder 24, forming a conduction current through an in-vitro animal skin 30 immersed between an upper cell body 2 and a lower cell body 22 filled with an upper electrolyte 38 and a lower electrolyte 7 inside, and measuring the transcutaneous resistance of the animal skin 30 by the resistance measuring instrument 34 according to an ohm in-vitro measuring law; the display screen 35 on the resistance measuring instrument 34 displays the measured value of the percutaneous resistance in real time, and the printer 36 on the top prints the measured value of the percutaneous resistance at any time. After the experiment is finished, the power switch of the resistance measuring instrument 34 is closed, and the outer electrode plug 27 connected with the outer electrode wire 29 of the resistance measuring instrument 34 is pulled out from the electrode interface 25 of the upper electrode 1 and the lower electrode 23; discharging the upper electrolyte 38 from the upper cell body 2 of the upper electrolytic cell 40 through the upper cell cylindrical opening 39, and discharging the lower electrolyte 7 from the lower cell body 22 of the lower electrolytic cell 15 through the lower cell cylindrical opening 6; when an operator rotates the screw handle 9 of the fastening screw 10 in one direction by hand, the upper screw 8 of the fastening screw 10 rotates to apply force to the upper clamp handle 5 in one direction by engaging force with the upper clamp internal thread 4, and at the same time, the lower screw 11 of the fastening screw 10 applies force to the lower clamp handle 13 in the other opposite direction by engaging force with the lower clamp internal thread 12 to form compression action of the upper clamp handle 5 and the lower clamp handle 13, because the upper clamp bracket 3 and the lower clamp bracket 18 are constrained in the bracket shaft 17 to form lever principle, expansion action of the upper clamp 32 and the lower clamp 21 is caused while the upper clamp handle 5 and the lower clamp handle 13 are clamped, and at the same time, the upper electrolytic cell 40 and the lower electrolytic cell 15 placed in the upper clamp opening 37 and the lower clamp opening 28 are loosened, and then the measured skin 30 of the excised animal is taken out of the lower cell body opening 19; putting a prepared piece of fresh in-vitro animal skin 30 which is coated with the tested medicine or is treated and tested for the experiment on the lower cell body opening 19 of the lower electrolytic cell 15; the above experiments are continuously repeated, and according to the comparison between each resistance value data and the normal percutaneous resistance value, whether the ex-vivo animal skin 12 which is coated with the tested medicine or is treated and tested and corresponds to the resistance value data for the fast experiment is corroded or damaged can be calculated, so that whether the tested medicine or the treated result coated on the ex-vivo animal skin 12 is corrosive and harmful to human beings or not is deduced, and the experimental data is subjected to the next scientific research and application.
Claims (6)
1. The utility model provides a simple and easy separation skin percutaneous resistance measuring device which characterized by: the device comprises a linkage clamping plate (14) which is provided with a fastening screw rod (10) and can control tightness degree and is similar to a movable clamp, an upper electrolytic cell (40) and a lower electrolytic cell (15) are fixedly arranged between an upper clamping plate port (37) and a lower clamping plate port (28) which are provided with notches at the front end of the linkage clamping plate (14), the uppermost edge of the upper electrolytic cell (40) is an upper cell cylinder port (39) for injecting or discharging upper electrolyte (38) at any time, the lowermost edge of the upper electrolytic cell (40) is an upper cell body port (31) with a smooth and flat opening towards the lower edge, an upper electrode (1) and an electrode interface (25) which are used for connecting an outer electrode line (29) output by a resistance measuring instrument (34) are arranged and fixed on the side wall of an upper cell body (2) at a certain distance away from the upper cell body port (31) for percutaneous resistance measurement, the uppermost edge of the lower electrolytic cell body (15) is a lower cell body port (19) with a smooth and flat opening towards the upper cell body edge, a lower cell body port (19) with a certain distance from the lower cell body port (19) for injecting or discharging lower cell body (19) by a return connection of an upper cell body (19), the lower cell body opening (19) which is a lower opening (19) which is a certain distance and is a lower opening of an extension tube which is a lower opening which is bent and extends from an upper cell body (7) which is fixed on the lower end of an upper cell body (7) and is fixed on the lower end of an upper cell body (19) which is fixed and is a lower cell body which is fixed on the lower cell body (19) and is a lower cell body which is a lower end of an upper cell body which is connected with a lower cell body (19) and is a certain distance, and is connected with a lower end of an upper cell body (7) which is connected with a lower cell body which is a lower end of an extension tube which is connected with a lower end of an extension tube (7) which is a lower end of an upper cell body (19) which is connected with a lower end of an extension tube which is a lower end of The electrode (23) and the electrode interface (25) are directly connected with an outer electrode wire (29) output by the resistance measuring instrument (34) to carry out percutaneous resistance measurement to complete the measurement of the percutaneous resistance of the skin of the isolated animal;
the linkage clamping plate (14) is made of a rigid corrosion-resistant material, is a mechanical device for clamping and fixing in-vitro animal skin (30) between an upper electrolytic cell (40) and a lower electrolytic cell (15), the front parts of an upper clamping plate (32) and a lower clamping plate (21) which are same in shape and opposite in direction are respectively provided with an upper clamping plate opening (37) and a lower clamping plate opening (28) which are opened forwards, the shapes and the sizes of the upper clamping plate opening (37) and the lower clamping plate opening (28) are just capable of accommodating and fixing the edges of an upper cell body (2) of the upper electrolytic cell (40) and a lower cell body (22) of the lower electrolytic cell (15), the middle parts of the upper clamping plate (32) and the lower clamping plate (21) are respectively provided with an upper clamping bracket (3) and a lower clamping bracket (18) which are same in shape and opposite in direction, the upper clamping bracket and the lower clamping bracket (18) are movably connected together through a bracket shaft (17), a bracket spring (16) is sleeved on the bracket shaft (17), the upper clamping plate handle (5) and the lower clamping plate handle (13) are movably connected together, the upper clamping plate handle (12) and the lower clamping plate (13) which are separated from each other, the upper clamping plate handle (12) and the lower clamping plate handle (12) which are same in the lower clamping bracket (13) are installed in the same in the upper clamping handle (13), a fastening screw rod (10) is engaged between the upper clamp internal thread (4) and the lower clamp internal thread (12), the distance between the upper clamp plate handle (5) and the lower clamp plate handle (13) can be adjusted at any time, and as the upper clamp bracket (3) and the lower clamp bracket (18) are respectively movably linked together through a bracket shaft (17), the purpose of pressing or loosening the in-vitro animal skin (30) between the upper electrolytic cell (40) and the lower electrolytic cell (15) can be achieved by changing the distance between the upper clamp plate port (37) and the lower clamp plate port (28) under the action of the lever principle;
the fastening screw rod (10) is a fastening element which is vertically arranged at the rear part of the linkage clamping plate (14) and made of firm corrosion-resistant materials, the middle part of the fastening screw rod (10) is a disk-shaped screw rod rotating handle which is rotated by hands, the center of the disk extends upwards and downwards to two ends respectively to form an upper screw rod (8) and a lower screw rod (11), the thread direction of the upper screw rod (8) is opposite to the thread direction of the lower screw rod (11), the thread of the upper screw rod (8) is meshed in an upper clamping internal thread (4) at the rear part of the linkage clamping plate (14), the thread of the lower screw rod (11) is meshed in a lower clamping internal thread (12) at the rear part of the linkage clamping plate (14), when an operator rotates the screw rod rotating handle (9) of the fastening screw rod (10) in one direction by hands, an upper screw (8) of a fastening screw (10) rotates to apply force to an upper clamping plate handle (5) in one direction through meshing force with an upper clamping internal thread (4), and simultaneously a lower screw (11) of the fastening screw (10) applies force to a lower clamping plate handle (13) in the other opposite direction through meshing force with a lower clamping internal thread (12) to form expansion or clamping action of the upper clamping plate handle (5) and the lower clamping plate handle (13), and an upper clamping bracket (3) and a lower clamping bracket (18) are constrained in a bracket shaft (17) to form a lever principle, so that the expansion or clamping action of the upper clamping plate handle (5) and the lower clamping plate handle (13) is simultaneously formed to the upper clamping plate (32) And the lower clamping plate (21) and the upper electrolytic cell (40) and the lower electrolytic cell (15) which are arranged in the upper clamping plate opening (37) and the lower clamping plate opening (28) are clamped or loosened simultaneously, so that the air tightness of experimental detection and convenience in disassembly are guaranteed.
2. The simple isolated skin percutaneous electrical resistance measurement device of claim 1, wherein: the upper electrolytic cell (40) is a cavity of an inverted cone-shaped funnel with smooth inner and outer walls made of a firm corrosion-resistant insulating material, the uppermost edge of the cavity of the inverted cone-shaped funnel is an upper cell tube opening (39) with a cylindrical upward opening, electrolyte (38) can be injected or discharged from the opening at any time, the cylinder below the upper cell tube opening (39) is communicated with the upper edge of an upper cell body (2) of the inverted cone-shaped funnel in an extending mode, the large opening edge of the inverted cone-shaped funnel at the bottommost edge of the upper cell body (2) is an upper cell body opening (31) with a smooth and flat opening edge, the diameter and the shape of the upper cell body opening (31) are completely identical to those of a lower cell body opening (19) of a lower electrolytic cell (15) arranged at the corresponding position and are aligned and matched with and pressed against the skin (30) of an in-vitro animal, the situation that the upper electrolyte (38) is injected into the upper electrolytic cell (40) cannot leak in a sealing mode is ensured, an outer electrode (1) and an electrode (25) are fixedly arranged on the side wall of the upper cell body (2) which is a certain distance away from the upper cell body opening (31) in parallel to the upper cell body opening (31), and an electrode output line (29) is connected with a resistance measuring instrument for measuring through a resistance measuring instrument for measuring resistor (34).
3. The simple isolated skin percutaneous electrical resistance measurement device of claim 1, wherein: the lower electrolytic cell (15) is a cavity of a conical funnel with an upward smooth large opening and an extended pipe (20) and an upward smooth inner wall and an outer wall made of a firm corrosion-resistant insulating material, the conical funnel part in the cavity of the conical funnel with the upward large opening is a lower cell body (22), a small opening edge at the lower part of the lower cell body (22) is extended and communicated with the extended pipe (20) with a bent and folded shape in a section of cylinder, the other end of the extended pipe (20) is a lower cell body opening (6) with an upward opening, lower electrolyte (7) can be injected or discharged from the opening at any time, a large opening edge of the upper conical funnel of the lower cell body (22) is a lower cell body opening (19) with a smooth and flat opening edge, the diameter and the shape of the lower cell body opening (19) are completely the same as those of an upper cell body opening (31) of an upper electrolytic cell body (40) arranged at the corresponding position, the upper cell body opening (31) is aligned and matched with the upper cell body opening (30) and tightly presses the skin (30) of an animal, the lower cell body (15) to ensure that the lower electrolytic cell body (15) is sealed without leakage when the lower electrolyte (7) is injected, and an upper electrode (19) and a lower electrode (23) is connected with a percutaneous resistance measuring instrument (34) which is parallel to be fixed and connected with the lower electrode (23) for percutaneous resistance measuring.
4. The simple isolated skin percutaneous electrical resistance measurement device of claim 1, wherein: the upper electrode (1) and the lower electrode (23) are both compact conductive nets made of metal materials with good conductivity, different conductive areas and diameters are selected according to the skin (30) of the isolated animal with different areas and diameters and corresponding requirements, the outer surfaces of the upper electrode (1) and the lower electrode (23) are smooth and straight to facilitate conduction and prevent pollution and corrosion, the upper electrode (1) and the lower electrode (23) are respectively and parallelly arranged on the upper tank body (2) and the lower tank body (22) at equal intervals with the skin (30) of the isolated animal in parallel, the upper electrode (1) and the lower electrode (23) are respectively provided with an electrode interface (25) at the edge of the respective tank body, the electrode interfaces (25) are connected with a resistance measuring instrument (34) through corresponding outer electrode wires (29), the resistance measuring instrument (34) is connected with a resistance measuring instrument (34) through the outer electrode wires (29), the electrode interfaces (25) and the upper electrode (1) and the lower electrode (23) which are respectively arranged in the upper electrolytic tank (40) and the lower electrolytic tank (15), the resistance measuring instrument (40) can accurately measure the isolated animal skin (30) which is fixed between the upper electrolytic tank (15), and the isolated animal can be operated through a resistance value display circuit or printed on a percutaneous data display panel (33) or a printer which can print the resistance data.
5. The simple isolated skin percutaneous electrical resistance measurement device of claim 1, wherein: the electrode interfaces (25) are a pair of electronic components which are made of good conductive and corrosion-resistant materials and are respectively fixedly arranged on the edges of the upper tank body (2) and the lower tank body (22), each electrode interface (25) is provided with an inner electrode seat (24) which penetrates through the edges of the upper tank body (2) and the lower tank body (22) and is sealed and arranged without any leakage or looseness, the inner electrode seats (24) are immersed in an upper electrolyte (38) and a lower electrolyte (7) which are filled in the upper tank body (2) and the lower tank body (22) and are fixedly connected with the upper electrode (1) and the lower electrode (23) to form conduction current through in-vitro animal skin, each electrode interface (25) is also provided with a pair of electrode seat holes (26) and an outer electrode plug (27) which can be inserted and disconnected at any time, the electrode seat holes (26) are communicated with an upper electrode (1) and a lower electrode (23) through an inner electrode seat (24), the outer electrode plug (27) is connected with a resistance measuring instrument (34) through an outer electrode wire (29), when the outer electrode plug (27) connected with the outer electrode wire (29) of the resistance measuring instrument (34) is inserted into the electrode seat holes (26), the upper electrode (1) and the lower electrode (23) communicated with the inner electrode seat (24) are immersed in-vitro animal skin between the upper cell body (2) and the lower cell body (22) filled with upper electrolyte (38) and lower electrolyte (7) inside through immersion (30) And forming a conduction current to complete the measurement of the value of the transcutaneous resistance.
6. The simple isolated skin percutaneous electrical resistance measurement device of claim 1, wherein: the resistance measuring instrument (34) is an electronic instrument which can measure the output voltage and current of the in-vitro animal skin (30) fixed on the linkage splint (14) through an external electrode wire (29) and then measure the percutaneous resistance of the in-vitro animal skin (30) according to ohm's law, an electronic instrument consisting of an intelligent computer control program circuit and a precise resistance measuring circuit is arranged in the resistance measuring instrument (34), the precise resistance measuring circuit arranged in the electronic instrument is connected to the external electrode wire (29) to precisely measure the resistance value of the in-vitro animal skin (30) fixed on the linkage splint (14), a display screen (35) is arranged on the upper part of the resistance measuring instrument (34) and can display the measured percutaneous resistance value and the set program at any time, an operation panel (33) is arranged on the front side of the resistance measuring instrument (34) and can conveniently set the measuring program and requirements, and a printer (36) is arranged on the top of the resistance measuring instrument (34) and can print the measured percutaneous resistance value and the set program at any time.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610827046.XA CN106199198B (en) | 2016-09-18 | 2016-09-18 | Simple in-vitro skin percutaneous resistance measuring device |
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| CN201610827046.XA CN106199198B (en) | 2016-09-18 | 2016-09-18 | Simple in-vitro skin percutaneous resistance measuring device |
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| CN106199198B true CN106199198B (en) | 2023-03-28 |
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| JP3745469B2 (en) * | 1996-10-25 | 2006-02-15 | 株式会社ポリトロニクス | Skin resistance measuring device |
| DE10311840B4 (en) * | 2003-03-18 | 2010-07-29 | Hellige, Gerhard, Prof. Dr.med. | Method and device for electrical impedance measurement of a body |
| TWI285739B (en) * | 2005-12-20 | 2007-08-21 | Univ Feng Chia | Electrochemical testing device |
| CN102475544A (en) * | 2010-11-29 | 2012-05-30 | 汕头大学医学院第一附属医院 | Electrocardiogram electrode clamp |
| CN102445602A (en) * | 2011-09-30 | 2012-05-09 | 广东省疾病预防控制中心 | In-vitro skin transcutaneous electrical resistance measuring device |
| CN203479904U (en) * | 2013-07-19 | 2014-03-12 | 中航锂电（洛阳）有限公司 | Resistance detection device for diaphragm of lithium battery |
| CN105116225A (en) * | 2015-08-19 | 2015-12-02 | 天津开发区合普工贸有限公司 | Full-automatic excised skin percutaneous resistance measuring device |
| CN206057440U (en) * | 2016-09-18 | 2017-03-29 | 天津开发区合普工贸有限公司 | A kind of simple isolated skin percutaneous electric resistance measuring apparatus |
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