CN106198954A - A kind of sample buffer for fluorescence immunoassay detection by quantitative - Google Patents
A kind of sample buffer for fluorescence immunoassay detection by quantitative Download PDFInfo
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- CN106198954A CN106198954A CN201610577963.7A CN201610577963A CN106198954A CN 106198954 A CN106198954 A CN 106198954A CN 201610577963 A CN201610577963 A CN 201610577963A CN 106198954 A CN106198954 A CN 106198954A
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- shaking
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Abstract
The invention discloses a kind of sample buffer for fluorescence immunoassay detection by quantitative, including the raw material of following weight portion: calf serum 25 30 parts, sodium dihydrogen phosphate 15 20 parts, disodium hydrogen phosphate 14 18 parts, deionized water 30 35 parts；Its preparation method comprises the following steps: S1: sodium dihydrogen phosphate and deionized water are mixed, and prepares solution A at 20 25 DEG C after shaking up；S2: disodium hydrogen phosphate and deionized water are mixed, prepares second liquid at 20 25 DEG C after shaking up；S3: liquid A and liquid B mixed, prepares PB buffer at 25 30 DEG C after shaking up；S4: calf serum and PB buffer are mixed, prepares sample buffer at 35 40 DEG C after shaking up.The present invention not only reduces the cost of product, and has good stability and repeatability, and can be preferably applied to fluorescence immunoassay quantitative analysis, and quickly, result is accurate, easy and simple to handle in detection.
Description
Technical field
The present invention relates to fluorescence immunoassay quantitative measurement technology field, particularly relate to a kind of for fluorescence immunoassay detection by quantitative
Sample buffer.
Background technology
Immunolabelling technique refers to make with fluorescein, radiosiotope, enzyme, ferritin, gold colloidal and chemiluminescence agent etc.
The antigen antibody reaction carried out for tracer, traget antibody or antigen, and by means of fluorescence microscope, ray meter, enzyme mark
The precision instruments such as detector, ultramicroscope and luminescent immunoassay instrument, apply various liquid phase and solid phase immunoassays formats, right
Hapten, antigen or antibody in body fluid carries out the qualitative and method of quantitative determination；Time resolved fluoro-immunoassay is a kind of
Sensitive high-end quantitative immunological detection technique, is to come labelled antigen or antibody as label, because of group of the lanthanides using La rear earth ion
Rare earth ion has the fluorescent characteristic of uniqueness, and this technology is obtained in that high signal to noise ratio, thus has the highest sensitivity, and
Also have that label preparation is easy, store time length, "dead" pollution, detect reproducible, operating process is short, standard curve
Wide ranges, not by the interference of sample natural fluorescence and the advantage such as range of application is quite varied；Time resolved fluoro-immunoassay is relative
Exempt from analysis in enzyme, radioimmunoassay, RIA has higher clinical value；This technology is applicable to the clinical inspection of various antigen-antibody
Survey, be commercially widely used.
Fluorescence immune chromatography is built upon elisa, latex agglutination test, monoclonal antibody technique and exempts from
On the basis of epidemic disease fluorescent microsphere labelling technique with fluorescent microsphere as label, utilize special antigen antibody reaction to amplify instead
Should, by the new technique of quantitative fluorescence analysis instrument result of determination；This technology has the advantages such as simple, quick, accurate and pollution-free,
Quickly detect many diagnostic fields at clinical medicine detection, hormone test, food safety detection, drug residue and drugs to send out rapidly
Exhibition；Along with the deep development of real-time test (POCT) industry, fluorescence immunoassay reagent due to itself with quick, easy,
Be easy to carry about with one, be easy to go deep into the feature such as community and outlying mountain area, become current POCT development major domain, thus those with send out
The demand of the fluorescence immunoassay reagent of the Testing index that disease is anxious, endanger big disease is correlated with is increasing.
But quantitatively chromatograph detection sample to carry out detecting remain the one of POCT field using whole blood sample as fluorescence immunoassay
Big focus and a difficult problem；Fluorescence immunoassay quantitatively chromatographs when using plasma/serum as detection sample, and testing result is highly sensitive, inspection
Survey method is easy, quick, specificity is high；But during using whole blood sample as detection sample, due to existence erythrocytic in whole blood,
During detection, once erythrocyte is leaked on test strips NC film, then can be to fluorescent microsphere gathering on NC film detection line and control line
Produce impact, thus cause false sun or the false negative of testing result；In view of separating serum/plasma and some operating procedures, extremely
Need about one hour less, and in the medical institutions without technical conditions, also cannot be carried out；Therefore, one whole blood is analyzed
The other diagnostic assay of bed, i.e. whole blood immunity chromatography Faxian is most important；For whole blood sample detection sample buffer also
Just become the fluorescence immunoassay quantitative testing test paper bar key in clinical practice.
Summary of the invention
The technical problem existed based on background technology, the present invention proposes a kind of sample for fluorescence immunoassay detection by quantitative
Buffer.
A kind of sample buffer for fluorescence immunoassay detection by quantitative that the present invention proposes, former including following weight portion
Material: calf serum 25-30 part, sodium dihydrogen phosphate 15-20 part, disodium hydrogen phosphate 14-18 part, deionized water 30-35 part；
Its preparation method comprises the following steps:
S1: sodium dihydrogen phosphate and deionized water are mixed, prepares solution A at 20-25 DEG C after shaking up；
S2: disodium hydrogen phosphate and deionized water are mixed, prepares second liquid at 20-25 DEG C after shaking up；
S3: liquid A and liquid B mixed, prepares PB buffer at 25-30 DEG C after shaking up；
S4: calf serum and PB buffer are mixed, prepares sample buffer at 35-40 DEG C after shaking up.
Preferably, including the raw material of following weight portion: calf serum 26-29 part, sodium dihydrogen phosphate 16-19 part, phosphoric acid
Disodium hydrogen 15-17 part, deionized water 31-34 part.
Preferably, in described S1, sodium dihydrogen phosphate and deionized water are mixed, after shaking up at 21-24 DEG C, prepare solution A.
Preferably, in described S2, disodium hydrogen phosphate and deionized water are mixed, after shaking up at 21-24 DEG C, prepare second liquid.
Preferably, in described S3, liquid A and liquid B is mixed, after shaking up at 26-29 DEG C, prepare PB buffer.
Preferably, in described S4, calf serum and PB buffer are mixed, prepare sample after shaking up at 36-39 DEG C and delay
Rush liquid.
In the present invention, described a kind of sample buffer for fluorescence immunoassay detection by quantitative adds PB buffering with calf serum
Liquid prepares sample buffer, it is possible to achieve detect using whole blood, blood plasma or serum as sample, with low cost, and to detection
Result is noiseless, and the present invention not only reduces the cost of product, and has good stability and repeatability, and can be more preferable
Ground is for fluorescence immunoassay quantitative analysis, and quickly, result is accurate, easy and simple to handle in detection.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is explained orally further.
Embodiment one
The present embodiment proposes a kind of sample buffer for fluorescence immunoassay detection by quantitative, including the raw material of following weight portion:
Calf serum 25 parts, sodium dihydrogen phosphate 15 parts, disodium hydrogen phosphate 14 parts, deionized water 30 parts；
Its preparation method comprises the following steps:
S1: sodium dihydrogen phosphate and deionized water are mixed, prepares solution A at 20 DEG C after shaking up；
S2: disodium hydrogen phosphate and deionized water are mixed, prepares second liquid at 20 DEG C after shaking up；
S3: liquid A and liquid B mixed, prepares PB buffer at 25 DEG C after shaking up；
S4: calf serum and PB buffer are mixed, prepares sample buffer at 35 DEG C after shaking up.
Embodiment two
The present embodiment proposes a kind of sample buffer for fluorescence immunoassay detection by quantitative, including the raw material of following weight portion:
Calf serum 28 parts, sodium dihydrogen phosphate 18 parts, disodium hydrogen phosphate 16 parts, deionized water 33 parts；
Its preparation method comprises the following steps:
S1: sodium dihydrogen phosphate and deionized water are mixed, prepares solution A at 23 DEG C after shaking up；
S2: disodium hydrogen phosphate and deionized water are mixed, prepares second liquid at 23 DEG C after shaking up；
S3: liquid A and liquid B mixed, prepares PB buffer at 28 DEG C after shaking up；
S4: calf serum and PB buffer are mixed, prepares sample buffer at 38 DEG C after shaking up.
Embodiment three
The present embodiment proposes a kind of sample buffer for fluorescence immunoassay detection by quantitative, including the raw material of following weight portion:
Calf serum 30 parts, sodium dihydrogen phosphate 20 parts, disodium hydrogen phosphate 18 parts, deionized water 35 parts；
Its preparation method comprises the following steps:
S1: sodium dihydrogen phosphate and deionized water are mixed, prepares solution A at 25 DEG C after shaking up；
S2: disodium hydrogen phosphate and deionized water are mixed, prepares second liquid at 25 DEG C after shaking up；
S3: liquid A and liquid B mixed, prepares PB buffer at 30 DEG C after shaking up；
S4: calf serum and PB buffer are mixed, prepares sample buffer at 40 DEG C after shaking up.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (6)
1. the sample buffer for fluorescence immunoassay detection by quantitative, it is characterised in that include the raw material of following weight portion: little
Ox blood serum 25-30 part, sodium dihydrogen phosphate 15-20 part, disodium hydrogen phosphate 14-18 part, deionized water 30-35 part；
Its preparation method comprises the following steps:
S1: sodium dihydrogen phosphate and deionized water are mixed, prepares solution A at 20-25 DEG C after shaking up；
S2: disodium hydrogen phosphate and deionized water are mixed, prepares second liquid at 20-25 DEG C after shaking up；
S3: liquid A and liquid B mixed, prepares PB buffer at 25-30 DEG C after shaking up；
S4: calf serum and PB buffer are mixed, prepares sample buffer at 35-40 DEG C after shaking up.
A kind of sample buffer for fluorescence immunoassay detection by quantitative the most according to claim 1, it is characterised in that include
The raw material of following weight portion: calf serum 26-29 part, sodium dihydrogen phosphate 16-19 part, disodium hydrogen phosphate 15-17 part, go from
Sub-water 31-34 part.
A kind of sample buffer for fluorescence immunoassay detection by quantitative the most according to claim 1, it is characterised in that described
In S1, sodium dihydrogen phosphate and deionized water are mixed, after shaking up at 21-24 DEG C, prepare solution A.
A kind of sample buffer for fluorescence immunoassay detection by quantitative the most according to claim 1, it is characterised in that described
In S2, disodium hydrogen phosphate and deionized water are mixed, after shaking up at 21-24 DEG C, prepare second liquid.
A kind of sample buffer for fluorescence immunoassay detection by quantitative the most according to claim 1, it is characterised in that described
In S3, liquid A and liquid B is mixed, after shaking up at 26-29 DEG C, prepare PB buffer.
A kind of sample buffer for fluorescence immunoassay detection by quantitative the most according to claim 1, it is characterised in that described
In S4, calf serum and PB buffer are mixed, after shaking up at 36-39 DEG C, prepare sample buffer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610577963.7A CN106198954A (en) | 2016-07-21 | 2016-07-21 | A kind of sample buffer for fluorescence immunoassay detection by quantitative |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1664584A (en) * | 2005-01-31 | 2005-09-07 | 上海市血液中心 | An antigen-antibody dilution liquid and quality controlled produce formed thereby |
| CN102680697A (en) * | 2011-03-10 | 2012-09-19 | 北京吉奥众艺科技有限公司 | Reagent kit for detecting troponin I and preparation and use method thereof |
| CN104730231A (en) * | 2015-03-26 | 2015-06-24 | 北京乐普医疗科技有限责任公司 | Sample buffering solution used for fluorescence immunoassay quantitative determination and application of sample buffering solution |
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2016
- 2016-07-21 CN CN201610577963.7A patent/CN106198954A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1664584A (en) * | 2005-01-31 | 2005-09-07 | 上海市血液中心 | An antigen-antibody dilution liquid and quality controlled produce formed thereby |
| CN102680697A (en) * | 2011-03-10 | 2012-09-19 | 北京吉奥众艺科技有限公司 | Reagent kit for detecting troponin I and preparation and use method thereof |
| CN104730231A (en) * | 2015-03-26 | 2015-06-24 | 北京乐普医疗科技有限责任公司 | Sample buffering solution used for fluorescence immunoassay quantitative determination and application of sample buffering solution |
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Application publication date: 20161207 |