CN106198544A - A kind of method for rapidly judging of high dietary-fiber rice - Google Patents
A kind of method for rapidly judging of high dietary-fiber rice Download PDFInfo
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- CN106198544A CN106198544A CN201610548535.1A CN201610548535A CN106198544A CN 106198544 A CN106198544 A CN 106198544A CN 201610548535 A CN201610548535 A CN 201610548535A CN 106198544 A CN106198544 A CN 106198544A
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Abstract
The invention discloses the method for rapidly judging of a kind of high dietary-fiber rice, the method comprises the following steps: (1) takes the polished rice grain of rice, boils；(2) extracted by organic solvent, fat, albumen and starch that protease hydrolysis and amylase hydrolysis are removed after ripening in polished rice grain successively, it is thus achieved that residue；(3) by the form of naked eye residue, it is possible to the residue retaining head rice grain form is judged to high dietary-fiber rice；Rice grain structure disappears, residue becomes white fluffy floccule to be judged to low dietary fiber rice；Dietary fiber content >=5% in described high dietary-fiber rice.The present invention is with the polished rice grain of rice that boils as object of study, more gentle processing method is used the fat in the grain of rice, albumen and starch to be gone out, and judge the height of dietary fiber content in rice by the mode of appearance of the grain of rice, not only method is simple, efficient, and result is accurate.
Description
Technical field
The present invention relates to functional type staple food grain evaluation of nutrition field, particularly relate to the quick judgement of a kind of high dietary-fiber rice
Method.
Background technology
Dietary fiber refers in the digested absorption of little enteral, and to degrade or Partial digestion at big enteral complete fermentation
The plant component of edibility or the general name of homology carbohydrate, be divided into the water-insoluble and big class of water-soluble fibre two.Meals
Fiber contains more low-yield, it is possible to allows the cholesterol in blood and glycemic control in desirable level, has many benefits to human body.If
The intake of human body dietary fiber is not enough, then cause constipation, colon cancer, appendicitis, diabetes, obesity, coronary heart disease, cholelithiasis
Can strengthen etc. the sickness rate of disease.In today that diet is the finest, the intake of human body dietary fiber is the most not enough, so
Increasing research is devoted to improve the content of dietary fiber in food.Wherein, grain dietary fiber is human body dietary fiber
One of main source, be the important content strengthening human body dietary fiber nutrient.
Oryza sativa L. is the staple food grain of world's population more than half, and south China most area is with Oryza sativa L. as staple food grain, but rice
But it is a kind of typical low dietary fiber, the staple food of hyperglycemic index, the quick rising of blood sugar for human body after eating, can be caused, too high
Post-prandial glycemia may cause the disorder of Regulation of blood glucose, and then cause the generation of diabetes and other diseases.There are some researches show, rice master
Food and East Asia and south east asia height type Ⅱdiabetes mellitus sickness rate significant correlation.
At present, adding dietary fiber in food is the Main Means supplementing human body dietary fiber intake.Research shows,
In the Semen Tritici aestivi flour being added with dietary fiber, the hydrolysis rate of its starch can significantly reduce；Illustrate that this food is a kind of useful
Food in the low-glycemic of health.But, for rice, the edible way that people commonly use is by rice particles
Eat after steaming and decocting, rather than make rice flour produts, be not easy to add the dietary fiber of external source in rice.Therefore, by kind
Selection-breeding improves the dietary fiber content of rice self and becomes the Main Means that rice dietary fiber nutrient is strengthened.
In prior art, the assay method of the dietary fiber of main flow have AOAC985.29, AOAC991.43,
AOAC2011.25；Wherein, AOAC991.43 specifically comprise the following steps that key step includes homogenate, is dried, pulverizing, sieves, goes
Fat, Deproteinization, destarching, dietary fiber reclaim, dietary fiber is dried and dietary fiber drying and other steps.
Said determination method has time-consumingly, complex operation, the shortcoming such as relatively costly, and the mensuration of grain dietary fiber is easily subject to
To the interference of starch, so the mensuration difficulty of grain dietary fiber is relatively big, it is unfavorable in breeding work the screening to material.
Summary of the invention
The invention provides the method for rapidly judging of a kind of high dietary-fiber rice, the method can quickly judge rice product
The height of dietary fiber content in kind, be a kind of simple, can batch processing rice, efficiently and accurately rice dietary fiber semidefinite
Metering method.
The method for rapidly judging of a kind of high dietary-fiber rice, comprises the following steps:
(1) take the polished rice grain of rice, boil；
(2) extracted by organic solvent, fat that protease hydrolysis and amylase hydrolysis are removed after ripening in polished rice grain successively
Fat, albumen and starch, it is thus achieved that residue；
(3) by the form of naked eye residue, it is possible to the residue retaining head rice grain form is judged to high meals
Fiber rice；Rice grain structure disappears, residue becomes white fluffy floccule to be judged to low dietary fiber rice；Described high meals are fine
Dietary fiber content >=5% in dimension rice.
One of main component of rice dietary fiber is Formation of Endosperm Cell Walls, and the high rice Formation of Endosperm Cell Walls of dietary fiber is relatively
Thickness, albuminous cell is more, removes the Formation of Endosperm Cell Walls intensity after starch, albumen and fat big, can maintain sky albuminous cell
Stereochemical structure so that be able to observe that complete white grain of rice form in appearance, this is the knot that the empty albuminous cell of microcosmic is cumulative
Really；But, the rice Formation of Endosperm Cell Walls that dietary fiber content is low is frivolous, after removing starch, albumen and fat, and Formation of Endosperm Cell Walls
Original grain of rice form can not be maintained, and the rice grain structure observed in appearance is laxed, it is impossible to retain original grain of rice form.
As preferably, in step (2), described organic solvent is normal hexane；Extraction to oil red detects fat-free remnants
Only.Removing rice fat by normal hexane extraction, processing method is gentle, will not cause the loss of non-starch polysaccharides(nsp).
As preferably, in step (2), the temperature of described protease hydrolysis is 37 DEG C, and the time is 1.5～2.5h；It is hydrolyzed into
Till Ponceau S dyeing detection is without vestiges of protein.
As preferably, in step (2), described amylase is α-amylase and amyloglucosidase.
As preferably, in step (2), the temperature of described amylase hydrolysis is 37 DEG C, and the time is 15～17h.
Removed the main macromole such as albumen and starch of rice by enzymatic isolation method, processing environment is gentle, is removing rice three
While big nutrient, dietary fiber can be retained largely.
As preferably, in step (2), after amylase hydrolysis, digest through ethanol purge, obtain residue.
Compared with prior art, the method have the advantages that
(1) present invention is with the polished rice grain of rice that boils as object of study, uses more gentle processing method by the grain of rice
Fat, albumen and starch are gone out, and judge the height of dietary fiber content in rice, not only side by the mode of appearance of the grain of rice
Method is simple, efficient, and result is accurate.
(2) the present invention relates to physical extraction and biological enzyme hydrolysis, process and downgrade gentleness, avoid rice meals as far as possible
The loss of fiber.
(3) the inventive method detection method is succinct, can carry out in batches, and the detection method comparing traditional classical needs not rely on
Spectrophotometer and chromatographic apparatus, and do not contact the corrosivity such as concentrated sulphuric acid and stain or toxic reagent, testing cost is cheap.
(4) the inventive method detection is quick, compares with classical way and eliminates sample homogenization, is dried, sieves and meals are fine
Dimension recovery and weigh, be greatly saved manpower and time cost.
Accompanying drawing explanation
Fig. 1 is the aspect graph after Japanese warm and fine high dietary-fiber mutant is processed by the invention in embodiment 1.
Fig. 2 be in embodiment 2 Japanese warm and fine high dietary-fiber mutant hybridization after F2For dietary fiber phenotype stable strain
Dietary fiber content scattergram.
Detailed description of the invention
Embodiment 1
The method for rapidly judging of a kind of high dietary-fiber rice, specifically includes following steps:
(1) take the fine seed of high dietary-fiber Mutant Rice seed and common rice Japan, peeling, be polished into polished rice；
(2) taking the 100mg polished rice grain of rice in test tube, add 1mL water, boiling water steaming and decocting is to the most edible；
(3) remove excessive moisture, in test tube, add normal hexane, repeatedly extract, fat cannot be detected to oil red
Fat；
(4) remove lixiviating solution, after solid phase being dried, in test tube, add the KCl-HCl of the protease that 2mL contains 200IU
Buffer (0.1M, pH=1.5), is placed in test tube in 37 DEG C of shaking baths (160rpm), proteolysis reaction 2 hours, to beautiful
Spring, red S can't detect protein residue；
(5) remove the buffer of proteolysis reaction, in test tube, add α-amylase and the 15IU that 4mL contains 200IU
The sodium maleate buffer (0.1M, pH=6.5) of amyloglucosidase, 37 DEG C of 200rpm waters bath with thermostatic control shake 16 hours,
Carry out Starch Hydrolysis reaction；
(6) remove the buffer of Starch Hydrolysis reaction, clean digestion residue solid phase with ethanol, obtain obtaining residue；
(7) residue is placed on surface plate, the form of naked eye residue, it is possible to retain the residual of head rice grain form
Excess is judged to high dietary-fiber rice；Rice grain structure disappears, residue becomes white fluffy floccule to be judged to low dietary fiber
Rice；Dietary fiber content >=5% in high dietary-fiber rice.
Using the present embodiment method to carry out the judgement of rice dietary fiber content height, result is as shown in Figure 1；And use
Dietary fiber content in above two rice and concrete carbohydrate composition are measured by AOAC991.43 method, result
As shown in table 1.
Table 1 rice anti-digestion component carbohydrate analysis
N: Japan is fine, C: rice high dietary-fiber mutant
Shown in Fig. 1 and Biao 1 result, the present embodiment method is used fast and accurate can really to judge meals in rice variety
The height of fiber content.
Embodiment 2
For further enlarged sample amount, the present embodiment hybridization fine to high dietary-fiber mutant and common rice Japan obtains
Stable strain in the height of dietary fiber content judge, and record dietary fiber with standard method (AOAC991.43)
Result contrasts.
The acquisition of material: high dietary-fiber mutant is hybridized with low dietary fiber mutant, it is thus achieved that F1For plant,
F1Selfing obtains F2For plant, F2Use the continuous selfing of single seed descent after strain, obtain the strain that dietary fiber phenotype is stable
130.
Morphological characteristic after using the method described in embodiment 1 to measure the polished rice grain of rice process of above-mentioned 130 strains；Use
AOAC991.43 method measures the dietary fiber content of the rice of 130 strains；Measurement result is as shown in Figure 2.
Result is as follows: as shown in Figure 2, uses the method described in embodiment 1 can distinguish high dietary-fiber rice accurately
Reach 86.7% with low dietary fiber rice, residue phenotype and high dietary fiber content dependency, reflect through method described in embodiment 1
The dietary fiber exact level of fixed high dietary-fiber strain is above 5%.
Claims (6)
1. the method for rapidly judging of a high dietary-fiber rice, it is characterised in that comprise the following steps:
(1) take the polished rice grain of rice, boil；
(2) extracted by organic solvent, fat that protease hydrolysis and amylase hydrolysis are removed after ripening in polished rice grain successively, egg
White and starch, it is thus achieved that residue；
(3) by the form of naked eye residue, it is possible to the residue retaining head rice grain form is judged to high dietary-fiber
Rice；Rice grain structure disappears, residue becomes white fluffy floccule to be judged to low dietary fiber rice；
Dietary fiber content >=5% in described high dietary-fiber rice.
2. method for rapidly judging as claimed in claim 1, it is characterised in that in step (2), described organic solvent is just own
Alkane；Extract detecting fat-free remnants to oil red.
3. method for rapidly judging as claimed in claim 1, it is characterised in that in step (2), the temperature of described protease hydrolysis
Being 37 DEG C, the time is 1.5～2.5h；Be hydrolyzed into Ponceau S dyeing detection without vestiges of protein till.
4. method for rapidly judging as claimed in claim 1, it is characterised in that in step (2), described amylase is α-amylase
And amyloglucosidase.
5. method for rapidly judging as claimed in claim 1, it is characterised in that in step (2), the temperature of described amylase hydrolysis
Being 37 DEG C, the time is 15～17h.
6. method for rapidly judging as claimed in claim 1, it is characterised in that in step (2), after amylase hydrolysis, through ethanol
Clean digestion, obtain residue.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102084963A (en) * | 2010-09-29 | 2011-06-08 | 吉林省农业机械研究院 | Method for preparing oat bran dietary fiber food |
| CN102150550A (en) * | 2011-01-17 | 2011-08-17 | 山东省农业科学院作物研究所 | Wheat breeding method for improving content of resistant starch of kernels |
| CN105192098A (en) * | 2015-10-13 | 2015-12-30 | 东北农业大学 | Method for simultaneously extracting fat and protein in unpolished rice bran layer with aqueous enzymatic method |
-
2016
- 2016-07-11 CN CN201610548535.1A patent/CN106198544B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102084963A (en) * | 2010-09-29 | 2011-06-08 | 吉林省农业机械研究院 | Method for preparing oat bran dietary fiber food |
| CN102150550A (en) * | 2011-01-17 | 2011-08-17 | 山东省农业科学院作物研究所 | Wheat breeding method for improving content of resistant starch of kernels |
| CN105192098A (en) * | 2015-10-13 | 2015-12-30 | 东北农业大学 | Method for simultaneously extracting fat and protein in unpolished rice bran layer with aqueous enzymatic method |
Non-Patent Citations (2)
| Title |
|---|
| AOAC: "Total, Soluble, and Insoluble Dietary Fiber in Foods", 《AOAC OFFICIAL METHOD》 * |
| 王磊等: "食品检验工基础知识", 《机械工业出版社》 * |
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