CN106198525A - A kind of discrimination method of curcumin - Google Patents
A kind of discrimination method of curcumin Download PDFInfo
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- CN106198525A CN106198525A CN201610543203.4A CN201610543203A CN106198525A CN 106198525 A CN106198525 A CN 106198525A CN 201610543203 A CN201610543203 A CN 201610543203A CN 106198525 A CN106198525 A CN 106198525A
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Abstract
The present invention relates to the discrimination method of a kind of curcumin, which solve the technical problem that existing curcumin identification efficiency is relatively low and relatively costly, its step is to take appropriate amount of sample, respectively with ethanol and diluted hydrochloric acid dissolution, obtains A and B solution, solution A one is drawn with glue head dropper, put on filter paper, wait to do, filter paper stained yellow, drip a concentrated sulphuric acid, produce darkviolet.Drawing A, B solution one with glue head dropper, add a small amount of boric acid powder or diluent after mixing, color significantly reddens, and can differentiate in cherry-red after drying for curcumin, to the composite can be widely applied to the qualification of curcumin.
Description
Technical field
The present invention relates to the discrimination method of a kind of material, especially relate to the discrimination method of a kind of curcumin.
Background technology
Curcumin is a kind of pigment extracted from zingiberaceous plant Rhizoma Curcumae Longae, it is also possible to extracts from other zingiberaceous plants, is
A kind of yellow acidity aldehydes matter, reducing agent stability is relatively strong, the most not fugitive color after coloring.
Pharmacological research finds that curcumin not only has AntiHIV1 RT activity, anti-leishmania, anti-cell distortion, suppression blood in recent years
Platelet is assembled and thrombosis, anti-renal damage etc. act on, can be by intending Adrenal Glucocorticoid sample effect, antagonism inflammatory mediator
With cytokine and the release of nuclear factor (NF-kB)；Scavenging activated oxygen, the lipid peroxide reduced in blood regulating liver-QI；Strengthen liver
Homogenate total antioxidant capacity and superoxide dismutase, glutathione peroxidase activity, that reduces in blood and liver is unnecessary
Fat；Suppression serum lactate dehydrogenase (SLD) (LDH), glutamic oxaloacetic transaminase, GOT (AST), glutamate pyruvate transaminase (ALT), creatine phosphokinase and trip
From the rising of fatty acid, present and good protect the liver effectiveness.
Foreign study finds, curcumin is in vivo and in vitro to various toxicants such as CCl4, aflatoxin B1, acetparaminosalol
The hepatic injury of phenol, cyclophosphamide induction all has protective action, significantly reduces the alkaline phosphatase of alcoholic liver damage (ALI) animal
Enzyme, r-glutamyl transpeptidase (r-GT) activity, prevents the generation of experimental fatty liver from can suppress inflammatory reaction, antioxidation, resisting
Rheumatoid effect.It is possible to prevent arthroncus, arthritis, effective again to cardiovascular disease, cancer etc., by increasing
Pay close attention to and use.
Current curcumin market is the most chaotic, and true and false difficulty is distinguished.The discriminating of existing curcumin the most all uses thin layer chromatography
Method is carried out, although this method sensitivity is higher, but complex steps, the used time is longer, and ethanol extraction is inefficient, and cost is relatively
Height, is therefore badly in need of a kind of method conveniently differentiating curcumin.
Summary of the invention
The present invention is aiming at the technical problem that existing curcumin identification efficiency is relatively low and relatively costly, it is provided that a kind of discriminating
The authentication method of the higher and lower-cost curcumin of efficiency.
The present invention provides the discrimination method of a kind of curcumin, and step is as follows:
(1) take two parts of the sample needing to identify, obtain solution A and solution B with ethanol and diluted hydrochloric acid dissolution respectively, if sample
Product can not be dissolved in ethanol and dilute hydrochloric acid simultaneously, then this sample is not curcumin, judges if it can, then carry out next step；
(2) draw a solution A with glue head dropper and dropped on filter paper, if not in yellow after filter paper exsiccation, then sentencing
This sample fixed is not curcumin, if in yellow, then drips a sodium hydroxide solution at yellow position, if not producing redness,
Then judge that sample is not curcumin, if producing redness, then carry out next step and judge；
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add boric acid powder
Or boric acid solution, if mixed liquor color can not significantly redden, then judge that sample is not curcumin, if mixed liquor color significantly becomes
Red, then the mixed liquor reddened is dried, finally in cherry-red, then proves that sample is curcumin.
Preferably, the discrimination method step of curcumin is as follows:
(1) take the two parts of each 0.5g of sample needing to identify, be 0.08 by 18～22ml ethanol and 18～22ml concentration respectively
～0.12mol/l diluted hydrochloric acid dissolution obtains solution A and solution B, if sample can not be dissolved in ethanol and dilute hydrochloric acid, then simultaneously
This sample is not curcumin, judges if it can, then carry out next step；
(2) draw a solution A with glue head dropper and dropped on filter paper, if not in yellow after filter paper exsiccation, then sentencing
This sample fixed is not curcumin, if in yellow, then drips the sodium hydroxide that concentration is 8%～12% at yellow position molten
Liquid, if not producing redness, then judging that sample is not curcumin, if producing redness, then carrying out next step and judging；
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add 0.1g boric acid
Powder or 2ml concentration are the boric acid solution of 50%, if mixed liquor color can not significantly redden, then judge that sample is not curcumin,
If mixed liquor color significantly reddens, then the mixed liquor reddened is dried at 60 DEG C～100 DEG C, finally in cherry-red,
Then prove that sample is curcumin.
Preferably, the discrimination method step of curcumin is as follows:
(1) take the two parts of each 0.5g of sample needing to identify, be the dilute salt of 0.1mol/l by 20ml ethanol and 20ml concentration respectively
Acid is dissolved and is obtained solution A and solution B, if sample can not be dissolved in ethanol and dilute hydrochloric acid simultaneously, then this sample is not Rhizoma Curcumae Longae
Element, judges if it can, then carry out next step；
(2) draw a solution A with glue head dropper and dropped on filter paper, if not in yellow after filter paper exsiccation, then sentencing
This sample fixed is not curcumin, if in yellow, then dripping a concentration at yellow position is the sodium hydroxide solution of 10%, if
Do not produce redness, then judge that sample is not curcumin, if producing redness, then carry out next step and judge；
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add 0.1g boric acid
Powder or 2ml concentration are the boric acid solution of 50%, if mixed liquor color can not significantly redden, then judge that sample is not curcumin,
If mixed liquor color significantly reddens, then the mixed liquor reddened is dried at 100 DEG C, finally in cherry-red, then proves
Sample is curcumin.
Preferably, filter paper is qualitative analysis filter paper.
The method step of discriminating curcumin provided by the present invention is very simple, by dripping solution several times and being dried
Drawing identification result, efficiency is higher and cost is relatively low.The filter paper that the present invention is taked is qualitative analysis filter paper, the reagent dripped
For analytical reagent, the selection of these reagent and Liang Junshi our company the most used are for the specific selection of curcumin.
Detailed description of the invention
The filter paper of the present invention is qualitative analysis filter paper, and the reagent used by discriminating is analytical reagent.
(1) molecular formula of curcumin is C21H20O6, and main chain is unsaturated aliphatic and aromatic group.Test through our company
Card, curcumin is water insoluble and ether, is dissolved in ethanol, dilute hydrochloric acid, therefore uses ethanol and dilute hydrochloric acid to dissolve sample respectively.
Take two parts of the sample needing to identify, obtain solution A and solution B with ethanol and diluted hydrochloric acid dissolution respectively, if sample can not be simultaneously
Be dissolved in ethanol and dilute hydrochloric acid, then this sample is not curcumin, judges if it can, then carry out next step；
(2) curcumin is yellow when neutral, acid, therefore draws a solution A with glue head dropper and dropped to filter paper
On, if not in yellow after filter paper exsiccation, then judging that this sample is not curcumin, if in yellow, then at yellow position, drip one
Drip sodium hydroxide solution (because curcumin is a polyphenol compound with beta-diketon structure, to occur in alkaline solution
The conjugation of electron cloud deviation, aobvious redness), if not producing redness, then judge that sample is not curcumin, if producing redness, then
Carry out next step to judge；
(3) curcumin is a kind of containing phenolic group with the natural drug of quinonyl, empirical tests its meet boron and the most substantially become red
Color.Draw each one and they mixed of A, B solution with glue head dropper, then in mixed liquor, add boric acid powder or boric acid is molten
Liquid, if mixed liquor color can not significantly redden, then judges that sample is not curcumin, if mixed liquor color significantly reddens, then will become
Red mixed liquor is dried (curcumin is poor at 40 DEG C of stability inferiors of ＞, and significant redness can be faded and be become cherry-red),
Finally in cherry-red, then prove that sample is curcumin.
Embodiment 1
(1) take the sample 0.5g needing to carry out identifying, dissolve with 20ml ethanol and obtain solution A；Take needs again to identify
Sample 0.5g, obtain solution B with 20ml diluted hydrochloric acid dissolution, dilute hydrochloric acid concentration used is 0.1mol/l.
(2) draw a solution A with glue head dropper and dropped on filter paper, in yellow after filter paper exsiccation, then at Huang
A color position droplet concentration of dropping is the sodium hydroxide solution of 10%, produces redness.
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add 0.1g boric acid
Powder, mixed liquor color significantly reddens.Then the mixed liquor reddened is dried, finally in cherry-red at 100 DEG C.
Verification calibration: this sample is curcumin.
Embodiment 2
(1) take the sample 0.5g needing to carry out identifying, dissolve with 18ml ethanol and obtain solution A；Take needs again to identify
Sample 0.5g, obtain solution B with 18ml diluted hydrochloric acid dissolution, dilute hydrochloric acid concentration used is 0.12mol/l.
(2) draw a solution A with glue head dropper and dropped on filter paper, in yellow after filter paper exsiccation, then at Huang
A color position droplet concentration of dropping is the sodium hydroxide solution of 12%, produces redness.
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add 2ml concentration
Being the boric acid solution of 50%, mixed liquor color significantly reddens.Then the mixed liquor reddened is dried, finally in Fructus Pruni pseudocerasi at 60 DEG C
Red.
Verification calibration: this sample is curcumin.
Embodiment 3
(1) take the sample 0.5g needing to carry out identifying, dissolve with 22ml ethanol and obtain solution A；Take needs again to identify
Sample 0.5g, obtain solution B with 22ml diluted hydrochloric acid dissolution, dilute hydrochloric acid concentration used is 0.08mol/l.
(2) draw a solution A with glue head dropper and dropped on filter paper, in yellow after filter paper exsiccation, then at Huang
A color position droplet concentration of dropping is the sodium hydroxide solution of 8%, produces redness.
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add 0.1g boric acid
Powder or 2ml concentration are the boric acid solution of 50%, and mixed liquor color significantly reddens.Then the mixed liquor reddened is done at 80 DEG C
Dry, finally in cherry-red.
Verification calibration: this sample is curcumin.
Embodiment 4
(1) take the sample 0.5g needing to carry out identifying, dissolve with 20ml ethanol and obtain solution A；Take needs again to identify
Sample 0.5g, obtain solution B with 20ml diluted hydrochloric acid dissolution, dilute hydrochloric acid concentration used is 0.1mol/l.Have relatively bottom solution A, B
Many insoluble matters.
(2) draw solution A one with glue head dropper and dropped on filter paper, in yellow after filter paper exsiccation, then at Huang
A color position droplet concentration of dropping is the sodium hydroxide solution of 10%, and filter paper changes without obvious color.
(3) draw each one and they mixed of A, B solution with glue head dropper, be subsequently adding 0.1g boric acid powder or 2ml
Concentration is the boric acid solution of 50%, and color is without significant change.
Verification calibration: this sample is not curcumin.
Claims (4)
1. a discrimination method for curcumin, is characterized in that step is as follows:
(1) take two parts of the sample needing to identify, obtain solution A and solution B with ethanol and diluted hydrochloric acid dissolution respectively, if sample is not
Can be dissolved in ethanol and dilute hydrochloric acid, then this sample is not curcumin simultaneously, judges if it can, then carry out next step；
(2) drawing a solution A with glue head dropper and dropped on filter paper, if not in yellow after filter paper exsiccation, then judging should
Sample is not curcumin, if in yellow, then dripping a sodium hydroxide solution at yellow position, if not producing redness, then sentencing
Random sample product are not curcumins, if producing redness, then carry out next step and judge；
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add boric acid powder or boron
Acid solution, if mixed liquor color can not significantly redden, then judges that sample is not curcumin, if mixed liquor color significantly reddens, then
The mixed liquor reddened is dried, finally in cherry-red, then proves that sample is curcumin.
2. the discrimination method of a curcumin, it is characterised in that step is as follows:
(1) take need identify two parts of each 0.5g of sample, respectively with 18～22ml ethanol and 18～22ml concentration be 0.08～
0.12mol/l diluted hydrochloric acid dissolution obtains solution A and solution B, if sample can not be dissolved in ethanol and dilute hydrochloric acid simultaneously, then should
Sample is not curcumin, judges if it can, then carry out next step；
(2) drawing a solution A with glue head dropper and dropped on filter paper, if not in yellow after filter paper exsiccation, then judging should
Sample is not curcumin, if in yellow, then at yellow position, droplet concentration of dropping is the sodium hydroxide solution of 8%～12%,
If not producing redness, then judging that sample is not curcumin, if producing redness, then carrying out next step and judging；
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add 0.1g boric acid powder
Or 2ml concentration is the boric acid solution of 50%, if mixed liquor color can not significantly redden, then judge that sample is not curcumin, if mixed
Close liquid color significantly to redden, then the mixed liquor reddened is dried at 60 DEG C～100 DEG C, finally in cherry-red, then demonstrate,proves
Bright sample is curcumin.
3. the discrimination method of a curcumin, it is characterised in that step is as follows:
(1) take the two parts of each 0.5g of sample needing to identify, be that 0.1mol/l dilute hydrochloric acid is molten by 20ml ethanol and 20ml concentration respectively
Solution obtains solution A and solution B, if sample can not be dissolved in ethanol and dilute hydrochloric acid simultaneously, then this sample is not curcumin, as
Fruit energy, then carry out next step and judge；
(2) drawing a solution A with glue head dropper and dropped on filter paper, if not in yellow after filter paper exsiccation, then judging should
Sample is not curcumin, if in yellow, then dripping a concentration at yellow position is the sodium hydroxide solution of 10%, if not producing
Raw redness, then judge that sample is not curcumin, if producing redness, then carries out next step and judges；
(3) draw each one and they mixed of A, B solution with glue head dropper, in mixed liquor, then add 0.1g boric acid powder
Or 2ml concentration is the boric acid solution of 50%, if mixed liquor color can not significantly redden, then judge that sample is not curcumin, if mixed
Close liquid color significantly to redden, then the mixed liquor reddened is dried at 100 DEG C, finally in cherry-red, then proves sample
For curcumin.
The discrimination method of curcumin the most according to claim 1, it is characterised in that described filter paper is qualitative analysis filter paper.
Priority Applications (1)
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| CN201610543203.4A CN106198525A (en) | 2016-06-28 | 2016-06-28 | A kind of discrimination method of curcumin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271594A (en) * | 2017-07-14 | 2017-10-20 | 河南中大恒源生物科技股份有限公司 | A kind of method for differentiating Natural Curcumin and synthesis curcumin |
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2016
- 2016-06-28 CN CN201610543203.4A patent/CN106198525A/en active Pending
Non-Patent Citations (1)
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| 钟明: "《食品医药有机化工产品分析测试大全（上）》", 31 May 2000 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271594A (en) * | 2017-07-14 | 2017-10-20 | 河南中大恒源生物科技股份有限公司 | A kind of method for differentiating Natural Curcumin and synthesis curcumin |
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