CN106191204A - A kind of method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance - Google Patents
A kind of method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance Download PDFInfo
- Publication number
- CN106191204A CN106191204A CN201610592991.6A CN201610592991A CN106191204A CN 106191204 A CN106191204 A CN 106191204A CN 201610592991 A CN201610592991 A CN 201610592991A CN 106191204 A CN106191204 A CN 106191204A
- Authority
- CN
- China
- Prior art keywords
- sample
- probiotic bacteria
- test tube
- bacterium solution
- sterilizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses the method for testing of a kind of probiotic bacteria anti-bacterial household antibacterial textile performance, comprise the steps: that staphylococcus aureus (ATCC 6538), escherichia coli (8099) bacterium solution are cultivated and prepare；Sample prepares；Sample sterilizing；Sample is bottled；" 0 " oscillating contact time of contact, dilution are cultivated and clump count；Result calculates.The present invention, by being positioned on test specimen and control sample in the buffer containing inoculation bacterium solution, by cultivating the mechanism of action that bacterial food chain is cut off by sample, is died of hunger, thus is reduced clump count.Under the temperature and humidity conditions of regulation, investigate the anti-microbial property of test sample with the bacteriostasis rate after cultivating 3 days (escherichia coli) and 6 days (staphylococcus aureus).
Description
Technical field
Present invention relates particularly to the method for testing of a kind of probiotic bacteria anti-bacterial household antibacterial textile performance.
Background technology
Due to tradition household articles raw material, produce, transport, sell in by a certain degree of environment and artificial contamination,
Thus cause product Residual contamination thing and pathogenic bacteria；The healthy hidden danger in use is brought to vast consumer；Tradition household articles
It is to produce in normal circumstances, harmful substance, dust in the humiture of nature, air, and household articles raw material is originally
Body just carries virus, antibacterial, chemical harmful substance etc.；Minority product even carries the carrier of virus and pathogenic bacteria.
Summary of the invention
Goal of the invention: the present invention provides the method for testing of a kind of probiotic bacteria anti-bacterial household antibacterial textile performance.
Technical scheme: the method for testing of a kind of probiotic bacteria anti-bacterial household antibacterial textile performance, comprises the steps:
(1) staphylococcus aureus (ATCC 6538), escherichia coli (8099) bacterium solution are cultivated and prepare
Preserve strain test tube slant from the antibacterial in 3 generations in generation 10 and take an inoculating loop antibacterial, nutrient agar panel is rule, enters
Row is cultivated, and cultivation temperature is 36 DEG C-38 DEG C, and incubation time is 18h 24h；From flat board, a typical bacterium is chosen with inoculating loop
Falling, be inoculated in 20mL nutrient broth, temperature is 36 DEG C-38 DEG C, and rotating speed is 130r/min, and shaken cultivation 18h 20h i.e. makes
Become inoculation bacteria suspension；Bacterium solution content uses spectrophotometer method to measure, and viable count should reach 1*109CFU/ml—5*109CFU/
Ml, this fresh bacterium solution should use in 4h as early as possible, to ensure the activity of inoculation bacterium；
Drawing 2mL 3mL from bacterial suspension with suction pipe, be staphylococcus aureus capping, escherichia coli remove the limit, and move
Enter in the test tube equipped with 9mL nutrient broth, fully mix；Draw 1ml and move in another test tube equipped with 9mL nutrient broth,
Fully mixing；Draw in the test tube that 1mL moves into equipped with 9mL0.03mol/L PBS, fully mix；Draw 5mL and move into dress
Have in the conical flask of 45mL0.03mol/L PBS, fully mix, be diluted to containing viable bacteria number 3*105CFU/mL—
4*105CFU/mL, 4 times thus fixing dilution programs, contain the nutrient broth of trace, are used for connecing sample in this inoculation bacterium solution
Kind, this inoculation bacterium solution should use in 4h as early as possible, to keep the activity of inoculation bacterium；
(2) sample prepares
Probiotic bacteria antibiotic fabric and untreated control sample thereof are cut into about 5mm*5mm size respectively, weigh 0.75g ± 0.05g
As a sample, wrapping with the little scraps of paper, need to weigh many points of samples according to experiment, every part of sample is all wrapped with the little scraps of paper；
(3) sample sterilizing
High-pressure sterilizing pot put into by the screw that will be equipped with sample, in 121 DEG C, 103kPa sterilizing 15min standby；
(4) sample bottling
Preparing 8 conical flasks, the wherein a probiotic bacteria antibiotic fabric of each addition in 4 flasks, in 4 flasks, each addition is not
Process control sample is a, then addition 70m L ± 0.1m L 0.03mol/L PBS in each flask；
(5) " 0 " oscillating contact time of contact, dilution are cultivated and clump count
Inoculate bacterium solution with suction pipe toward 5ml of addition each in 8 sample flasks, build bottle stopper, then these 8 flasks are placed in constant temperature oscillation
On device, at 24 DEG C ± 1 DEG C, with 100r/min 150r/min, after vibration 1min ± 5s, draw from each flask 1mL ±
0.1mL test solution, moves in the test tube equipped with 9mL ± 0.1mL 0.03mol/L PBS, fully mixes；With 10 times of dilutions
Method carries out 1 dilution again, fully mixes；From the test tube of each extension rate, draw 1mL ± 0.1mL immigration respectively with suction pipe to go out
The plate of bacterium, pours into nutrient agar 15mL, and the imbibition respectively of the test tube of each extension rate makes two flat boards and counts
Number, i.e. the two clump count difference should be within 15%, otherwise data invalid, room temperature solidify, be inverted flat board, with 37 DEG C ± 1 DEG C
Probiotic bacteria processes cultivates escherichia coli 72h, staphylococcus 144h on fabric, record the clump count of each flat board；Wherein, untreated
Control sample inoculates " 0 " time of contact, dilutes in 100 times of flat boards, and clump count controls, between 200CFU 250CFU, otherwise to affect
Test degree of accuracy；
(6) result calculates
As a example by separating the probiotic bacteria pure counting staphylococcus of extraction, bacteriostasis rate is calculated by formula (A.1):
Y=* 100 (A.1)
In formula:
Y bacteriostasis rate, unit is %；
A arranged the staphylococcus aureus number on sample cloth through probiotic bacteria after 6 days, and unit is (CFU/mL)；
B does not arranges the staphylococcus aureus number on control sample cloth after 6 days, unit is (CFU/mL).
As optimization: composition and the content of described nutrient broth are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Deionized water
1000ml；After sterilizing, pH value is 6.8 ± 0.2.
As optimization: composition and the content of described nutrient agar are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Agar powder
15g；Deionized water 1000ml；After sterilizing, pH value is 6.8 ± 0.2.
As optimization: composition and the content of described PBS are as follows: disodium hydrogen phosphate 2.84g；Potassium dihydrogen phosphate
1.36g；Deionized water 1000ml；After sterilizing, pH value is 7.2 7.4, saves backup at a temperature of 5 DEG C 10 DEG C.
Beneficial effect: the present invention is by being positioned on test specimen and control sample in the buffer containing inoculation bacterium solution, logical
Cross and cultivate the mechanism of action that bacterial food chain is cut off by sample, died of hunger, thus reduce clump count.Humiture bar in regulation
Under part, investigate the antibiotic property of test sample with the bacteriostasis rate after cultivating 3 days (escherichia coli) and 6 days (staphylococcus aureus)
Energy.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be described in detail.
Specific embodiment one:
The method of testing of a kind of probiotic bacteria anti-bacterial household antibacterial textile performance, comprises the steps:
(1) staphylococcus aureus (ATCC 6538), escherichia coli (8099) bacterium solution are cultivated and prepare
Preserve strain test tube slant from the antibacterial in 3 generations in generation 10 and take an inoculating loop antibacterial, nutrient agar panel is rule, enters
Row is cultivated, and cultivation temperature is 36 DEG C, and incubation time is 18h；From flat board, choose a colonies typical with inoculating loop, be inoculated in
In 20mL nutrient broth, temperature is 36 DEG C, and rotating speed is 130r/min, shaken cultivation 18h, is i.e. made for inoculating bacteria suspension；Bacterium solution
Content uses spectrophotometer method to measure, and viable count should reach 1*109CFU/ml, this fresh bacterium solution should use in 4h as early as possible,
To ensure the activity of inoculation bacterium；
Drawing 2mL from bacterial suspension with suction pipe, be staphylococcus aureus capping, escherichia coli remove the limit, and move into dress
Have in the test tube of 9mL nutrient broth, fully mix；Draw 1ml and move in another test tube equipped with 9mL nutrient broth, fully
Mixing；Draw in the test tube that 1mL moves into equipped with 9mL0.03mol/L PBS, fully mix；Draw 5mL move into equipped with
In the conical flask of 45mL0.03mol/L PBS, fully mix, be diluted to containing viable bacteria number 3*105CFU/mL, thus
4 times fixing dilution programs, contain the nutrient broth of trace in this inoculation bacterium solution, be used for inoculating sample, and this inoculation bacterium solution should
Use as early as possible in 4h, to keep the activity of inoculation bacterium；
(2) sample prepares
Probiotic bacteria antibiotic fabric and untreated control sample thereof are cut into about 5mm*5mm size respectively, weigh 0.70g as portion
Sample, wraps with the little scraps of paper, needs to weigh many points of samples according to experiment, and every part of sample is all wrapped with the little scraps of paper；
(3) sample sterilizing
High-pressure sterilizing pot put into by the screw that will be equipped with sample, in 121 DEG C, 103kPa sterilizing 15min standby；
(4) sample bottling
Preparing 8 conical flasks, the wherein a probiotic bacteria antibiotic fabric of each addition in 4 flasks, in 4 flasks, each addition is not
Process control sample is a, then an addition 69.9m L 0.03mol/L PBS in each flask；
(5) " 0 " oscillating contact time of contact, dilution are cultivated and clump count
Inoculate bacterium solution with suction pipe toward 5ml of addition each in 8 sample flasks, build bottle stopper, then these 8 flasks are placed in constant temperature oscillation
On device, at 23 DEG C, with 100r/min, after vibration 55s, from each flask, draw 0.9mL test solution, move into equipped with 8.9mL
In the test tube of 0.03mol/L PBS, fully mix；Carry out 1 dilution again with 10 times of dilution methods, fully mix；With suction
Pipe is drawn 0.9mL from the test tube of each extension rate respectively and is moved into the plate of sterilizing, pours into nutrient agar 15mL, often
The imbibition respectively of the test tube of individual extension rate makes two flat boards and counts, and i.e. the clump count difference of the two should be no within 15%
Then data invalid, room temperature solidifies, and is inverted flat board, processes cultivation escherichia coli 72h, staphylococcus on fabric with 36 DEG C at probiotic bacteria
144h, records the clump count of each flat board；Wherein, " 0 " time of contact inoculated by untreated control sample, dilutes in 100 times of flat boards, bacterium
The numerical control system that falls is between 200CFU, and otherwise degree of accuracy is tested in impact；
(6) result calculates
As a example by separating the probiotic bacteria pure counting staphylococcus of extraction, bacteriostasis rate is calculated by formula (A.1):
Y=* 100 (A.1)
In formula:
Y bacteriostasis rate, unit is %；
A arranged the staphylococcus aureus number on sample cloth through probiotic bacteria after 6 days, and unit is (CFU/mL)；
B does not arranges the staphylococcus aureus number on control sample cloth after 6 days, unit is (CFU/mL).
Composition and the content of described nutrient broth are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Deionized water 1000ml；After sterilizing,
PH value is 6.6.
Composition and the content of described nutrient agar are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Agar powder 15g；Deionization
Water 1000ml；After sterilizing, pH value is 6.6.
Composition and the content of described PBS are as follows: disodium hydrogen phosphate 2.84g；Potassium dihydrogen phosphate 1.36g；Deionization
Water 1000ml；After sterilizing, pH value is 7.2, saves backup at a temperature of 5 DEG C.
Specific embodiment two:
The method of testing of a kind of probiotic bacteria anti-bacterial household antibacterial textile performance, comprises the steps:
(1) staphylococcus aureus (ATCC 6538), escherichia coli (8099) bacterium solution are cultivated and prepare
Preserve strain test tube slant from the antibacterial in 3 generations in generation 10 and take an inoculating loop antibacterial, nutrient agar panel is rule, enters
Row is cultivated, and cultivation temperature is 38 DEG C, and incubation time is 24h；From flat board, choose a colonies typical with inoculating loop, be inoculated in
In 20mL nutrient broth, temperature is 38 DEG C, and rotating speed is 130r/min, shaken cultivation 20h, is i.e. made for inoculating bacteria suspension；Bacterium solution
Content uses spectrophotometer method to measure, and viable count should reach 5*109CFU/ml, this fresh bacterium solution should use in 4h as early as possible,
To ensure the activity of inoculation bacterium；
Drawing 3mL from bacterial suspension with suction pipe, be staphylococcus aureus capping, escherichia coli remove the limit, and move into dress
Have in the test tube of 9mL nutrient broth, fully mix；Draw 1ml and move in another test tube equipped with 9mL nutrient broth, fully
Mixing；Draw in the test tube that 1mL moves into equipped with 9mL0.03mol/L PBS, fully mix；Draw 5mL move into equipped with
In the conical flask of 45mL0.03mol/L PBS, fully mix, be diluted to containing viable bacteria number 4*105CFU/mL, thus
4 times fixing dilution programs, contain the nutrient broth of trace in this inoculation bacterium solution, be used for inoculating sample, and this inoculation bacterium solution should
Use as early as possible in 4h, to keep the activity of inoculation bacterium；
(2) sample prepares
Probiotic bacteria antibiotic fabric and untreated control sample thereof are cut into about 5mm*5mm size respectively, weigh 0.80g as portion
Sample, wraps with the little scraps of paper, needs to weigh many points of samples according to experiment, and every part of sample is all wrapped with the little scraps of paper；
(3) sample sterilizing
High-pressure sterilizing pot put into by the screw that will be equipped with sample, in 121 DEG C, 103kPa sterilizing 15min standby；
(4) sample bottling
Preparing 8 conical flasks, the wherein a probiotic bacteria antibiotic fabric of each addition in 4 flasks, in 4 flasks, each addition is not
Process control sample is a, then an addition 70.1mL 0.03mol/L PBS in each flask；
(5) " 0 " oscillating contact time of contact, dilution are cultivated and clump count
Inoculate bacterium solution with suction pipe toward 5ml of addition each in 8 sample flasks, build bottle stopper, then these 8 flasks are placed in constant temperature oscillation
On device, at 25 DEG C, with 150r/min, after vibration 1min ± 5s, from each flask, draw 1.1mL test solution, move into equipped with 9.1mL
In the test tube of 0.03mol/L PBS, fully mix；Carry out 1 dilution again with 10 times of dilution methods, fully mix；With suction
Pipe is drawn 1.1mL from the test tube of each extension rate respectively and is moved into the plate of sterilizing, pours into nutrient agar 15mL, often
The imbibition respectively of the test tube of individual extension rate makes two flat boards and counts, and i.e. the clump count difference of the two should be no within 15%
Then data invalid, room temperature solidifies, and is inverted flat board, processes cultivation escherichia coli 72h, staphylococcus on fabric with 38 DEG C at probiotic bacteria
144h, records the clump count of each flat board；Wherein, " 0 " time of contact inoculated by untreated control sample, dilutes in 100 times of flat boards, bacterium
The numerical control system that falls is between 250CFU, and otherwise degree of accuracy is tested in impact；
(6) result calculates
As a example by separating the probiotic bacteria pure counting staphylococcus of extraction, bacteriostasis rate is calculated by formula (A.1):
Y=* 100 (A.1)
In formula:
Y bacteriostasis rate, unit is %；
A arranged the staphylococcus aureus number on sample cloth through probiotic bacteria after 6 days, and unit is (CFU/mL)；
B does not arranges the staphylococcus aureus number on control sample cloth after 6 days, unit is (CFU/mL).
Composition and the content of described nutrient broth are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Deionized water 1000ml；After sterilizing,
PH value is 7.0.
Composition and the content of described nutrient agar are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Agar powder 15g；Deionization
Water 1000ml；After sterilizing, pH value is 7.0.
Composition and the content of described PBS are as follows: disodium hydrogen phosphate 2.84g；Potassium dihydrogen phosphate 1.36g；Deionization
Water 1000ml；After sterilizing, pH value is 7.4, saves backup at a temperature of 10 DEG C.
Specific embodiment three:
The method of testing of a kind of probiotic bacteria anti-bacterial household antibacterial textile performance, comprises the steps:
(1) staphylococcus aureus (ATCC 6538), escherichia coli (8099) bacterium solution are cultivated and prepare
Preserve strain test tube slant from the antibacterial in 3 generations in generation 10 and take an inoculating loop antibacterial, nutrient agar panel is rule, enters
Row is cultivated, and cultivation temperature is 37 DEG C, and incubation time is 21h；From flat board, choose a colonies typical with inoculating loop, be inoculated in
In 20mL nutrient broth, temperature is 37 DEG C, and rotating speed is 130r/min, shaken cultivation 19h, is i.e. made for inoculating bacteria suspension；Bacterium solution
Content uses spectrophotometer method to measure, and viable count should reach 3*109CFU/ml, this fresh bacterium solution should use in 4h as early as possible,
To ensure the activity of inoculation bacterium；
Drawing 2.5mL from bacterial suspension with suction pipe, be staphylococcus aureus capping, escherichia coli remove the limit, and move into
In test tube equipped with 9mL nutrient broth, fully mix；Draw 1ml to move in another test tube equipped with 9mL nutrient broth, fill
Divide mixing；Draw in the test tube that 1mL moves into equipped with 9mL0.03mol/L PBS, fully mix；Draw 5mL move into equipped with
In the conical flask of 45mL0.03mol/L PBS, fully mix, be diluted to containing viable bacteria number 3.5*105CFU/mL, by
These 4 times fixing dilution programs, contain the nutrient broth of trace, are used for inoculating sample in this inoculation bacterium solution, this inoculates bacterium solution
Should use as early as possible in 4h, to keep the activity of inoculation bacterium；
(2) sample prepares
Probiotic bacteria antibiotic fabric and untreated control sample thereof are cut into about 5mm*5mm size respectively, weigh 0.75g as portion
Sample, wraps with the little scraps of paper, needs to weigh many points of samples according to experiment, and every part of sample is all wrapped with the little scraps of paper；
(3) sample sterilizing
High-pressure sterilizing pot put into by the screw that will be equipped with sample, in 121 DEG C, 103kPa sterilizing 15min standby；
(4) sample bottling
Preparing 8 conical flasks, the wherein a probiotic bacteria antibiotic fabric of each addition in 4 flasks, in 4 flasks, each addition is not
Process control sample is a, then an addition 70mL 0.03mol/L PBS in each flask；
(5) " 0 " oscillating contact time of contact, dilution are cultivated and clump count
Inoculate bacterium solution with suction pipe toward 5ml of addition each in 8 sample flasks, build bottle stopper, then these 8 flasks are placed in constant temperature oscillation
On device, at 24 DEG C, with 100r/min, after vibration 1min, from each flask, draw 1mL ± 0.1mL test solution, move into equipped with 9mL
In the test tube of 0.03mol/L PBS, fully mix；Carry out 1 dilution again with 10 times of dilution methods, fully mix；With suction
Pipe is drawn 1mL from the test tube of each extension rate respectively and is moved into the plate of sterilizing, pours into nutrient agar 15mL, each
The imbibition respectively of the test tube of extension rate makes two flat boards and counts, and i.e. the clump count difference of the two should be within 15%, otherwise
Data invalid, room temperature solidifies, and is inverted flat board, processes cultivation escherichia coli 72h, staphylococcus on fabric with 37 DEG C at probiotic bacteria
144h, records the clump count of each flat board；Wherein, " 0 " time of contact inoculated by untreated control sample, dilutes in 100 times of flat boards, bacterium
The numerical control system that falls is between 230CFU, and otherwise degree of accuracy is tested in impact；
(6) result calculates
As a example by separating the probiotic bacteria pure counting staphylococcus of extraction, bacteriostasis rate is calculated by formula (A.1):
Y=* 100 (A.1)
In formula:
Y bacteriostasis rate, unit is %；
A arranged the staphylococcus aureus number on sample cloth through probiotic bacteria after 6 days, and unit is (CFU/mL)；
B does not arranges the staphylococcus aureus number on control sample cloth after 6 days, unit is (CFU/mL).
Composition and the content of described nutrient broth are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Deionized water 1000ml；After sterilizing,
PH value is 6.8.
Composition and the content of described nutrient agar are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Agar powder 15g；Deionization
Water 1000ml；After sterilizing, pH value is 6.8.
Composition and the content of described PBS are as follows: disodium hydrogen phosphate 2.84g；Potassium dihydrogen phosphate 1.36g；Deionization
Water 1000ml；After sterilizing, pH value is 7.3, saves backup at a temperature of 8 DEG C.
The present invention will be positioned on test specimen and control sample in the buffer containing inoculation bacterium solution, by cultivating sample
The mechanism of action cut off by bacterial food chain, is died of hunger, thus is reduced clump count.Under the temperature and humidity conditions of regulation, with training
Support the bacteriostasis rate after 3 days (escherichia coli) and 6 days (staphylococcus aureus) and investigate the anti-microbial property of test sample.
Staphylococcus aureus (ATCC 6538) that this test is used, escherichia coli (8099) can be to personnel or environment
Having harm, therefore test should be trained and experienced testing staff is engaged in by through correlation detection technology.Institute do not pointed out in this annex
Possible safety problem.User is had a responsibility for taking suitable safe and healthy measure and concrete measure seemingly, and ensures compliance with
Country is about the condition of rules and regulations.
Probiotic bacteria anti-bacterial household textile is divided into A level, AA level, three antibacterial ranks of AAA level by water-fastness number of times difference.Right
The bacteriostasis rate index of test probiotic bacteria anti-bacterial household textile see table 1.
The bacteriostasis rate index of table 1 probiotic bacteria anti-bacterial household textile
The present invention is not limited to above-mentioned preferred forms, and anyone can draw other various forms under the enlightenment of the present invention
Product, no matter but in its shape or structure, make any change, every have same as the present application or akin technical side
Case, within all falling within protection scope of the present invention.
Claims (4)
1. the method for testing of a probiotic bacteria anti-bacterial household antibacterial textile performance, it is characterised in that: comprise the steps:
(1) staphylococcus aureus (ATCC 6538), escherichia coli (8099) bacterium solution are cultivated and prepare
Preserve strain test tube slant from the antibacterial in 3 generations in generation 10 and take an inoculating loop antibacterial, nutrient agar panel is rule, enters
Row is cultivated, and cultivation temperature is 36 DEG C-38 DEG C, and incubation time is 18h 24h；From flat board, a typical bacterium is chosen with inoculating loop
Falling, be inoculated in 20mL nutrient broth, temperature is 36 DEG C-38 DEG C, and rotating speed is 130r/min, and shaken cultivation 18h 20h i.e. makes
Become inoculation bacteria suspension；Bacterium solution content uses spectrophotometer method to measure, and viable count should reach 1*109CFU/ml—5*109CFU/
Ml, this fresh bacterium solution should use in 4h as early as possible, to ensure the activity of inoculation bacterium；
Drawing 2mL 3mL from bacterial suspension with suction pipe, be staphylococcus aureus capping, escherichia coli remove the limit, and move
Enter in the test tube equipped with 9mL nutrient broth, fully mix；Draw 1ml and move in another test tube equipped with 9mL nutrient broth,
Fully mixing；Draw in the test tube that 1mL moves into equipped with 9mL0.03mol/L PBS, fully mix；Draw 5mL and move into dress
Have in the conical flask of 45mL0.03mol/L PBS, fully mix, be diluted to containing viable bacteria number 3*105CFU/mL—
4*105CFU/mL, 4 times thus fixing dilution programs, contain the nutrient broth of trace, are used for connecing sample in this inoculation bacterium solution
Kind, this inoculation bacterium solution should use in 4h as early as possible, to keep the activity of inoculation bacterium；
(2) sample prepares
Probiotic bacteria antibiotic fabric and untreated control sample thereof are cut into about 5mm*5mm size respectively, weigh 0.75g ± 0.05g
As a sample, wrapping with the little scraps of paper, need to weigh many points of samples according to experiment, every part of sample is all wrapped with the little scraps of paper；
(3) sample sterilizing
High-pressure sterilizing pot put into by the screw that will be equipped with sample, in 121 DEG C, 103kPa sterilizing 15min standby；
(4) sample bottling
Preparing 8 conical flasks, the wherein a probiotic bacteria antibiotic fabric of each addition in 4 flasks, in 4 flasks, each addition is not
Process control sample is a, then addition 70m L ± 0.1m L 0.03mol/L PBS in each flask；
(5) " 0 " oscillating contact time of contact, dilution are cultivated and clump count
Inoculate bacterium solution with suction pipe toward 5ml of addition each in 8 sample flasks, build bottle stopper, then these 8 flasks are placed in constant temperature oscillation
On device, at 24 DEG C ± 1 DEG C, with 100r/min 150r/min, after vibration 1min ± 5s, draw from each flask 1mL ±
0.1mL test solution, moves in the test tube equipped with 9mL ± 0.1mL 0.03mol/L PBS, fully mixes；With 10 times of dilutions
Method carries out 1 dilution again, fully mixes；From the test tube of each extension rate, draw 1mL ± 0.1mL immigration respectively with suction pipe to go out
The plate of bacterium, pours into nutrient agar 15mL, and the imbibition respectively of the test tube of each extension rate makes two flat boards and counts
Number, i.e. the two clump count difference should be within 15%, otherwise data invalid, room temperature solidify, be inverted flat board, with 37 DEG C ± 1 DEG C
Probiotic bacteria processes cultivates escherichia coli 72h, staphylococcus 144h on fabric, record the clump count of each flat board；Wherein, untreated
Control sample inoculates " 0 " time of contact, dilutes in 100 times of flat boards, and clump count controls, between 200CFU 250CFU, otherwise to affect
Test degree of accuracy；
(6) result calculates
As a example by separating the probiotic bacteria pure counting staphylococcus of extraction, bacteriostasis rate is calculated by formula (A.1):
Y=* 100 (A.1)
In formula:
Y bacteriostasis rate, unit is %；
A arranged the staphylococcus aureus number on sample cloth through probiotic bacteria after 6 days, and unit is (CFU/mL)；
B does not arranges the staphylococcus aureus number on control sample cloth after 6 days, unit is (CFU/mL).
The method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance the most according to claim 1, it is characterised in that: institute
Composition and the content of stating nutrient broth are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Deionized water 1000ml；After sterilizing, pH value is 6.8
±0.2。
The method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance the most according to claim 1, it is characterised in that: institute
Composition and the content of stating nutrient agar are as follows: Carnis Bovis seu Bubali cream 3g；Peptone 5g；Agar powder 15g；Deionized water 1000ml；
After sterilizing, pH value is 6.8 ± 0.2.
The method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance the most according to claim 1, it is characterised in that: institute
Composition and the content of stating PBS are as follows: disodium hydrogen phosphate 2.84g；Potassium dihydrogen phosphate 1.36g；Deionized water 1000ml；Go out
After bacterium, pH value is 7.2 7.4, saves backup at a temperature of 5 DEG C 10 DEG C.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610592991.6A CN106191204A (en) | 2016-07-26 | 2016-07-26 | A kind of method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance |
| PCT/CN2016/105675 WO2018018784A1 (en) | 2016-07-26 | 2016-11-14 | Method for testing antibacterial performance of probiotic antibacterial home textile |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610592991.6A CN106191204A (en) | 2016-07-26 | 2016-07-26 | A kind of method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106191204A true CN106191204A (en) | 2016-12-07 |
Family
ID=57495957
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610592991.6A Pending CN106191204A (en) | 2016-07-26 | 2016-07-26 | A kind of method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN106191204A (en) |
| WO (1) | WO2018018784A1 (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207554A (en) * | 2018-09-06 | 2019-01-15 | 西安开米股份有限公司 | The method for quickly detecting daily chemical products fungistatic effect with TTC agar medium |
| CN109402216A (en) * | 2017-08-17 | 2019-03-01 | 宝洁公司 | Method for demonstrating the efficacy against bacteria of toothbrush bristle |
| CN109609590A (en) * | 2018-12-21 | 2019-04-12 | 扬州倍加洁日化有限公司 | A kind of anti-microbial property test method of wet tissue product |
| CN109762873A (en) * | 2019-03-27 | 2019-05-17 | 苏州纳工坊健康科技有限公司 | A kind of fungicide antibacterial effect detection method and fungicide storing unit |
| CN110272969A (en) * | 2019-03-15 | 2019-09-24 | 李文杰 | ATP bioluminescence lgCA-lgIAThe method of calibration curve method evaluation chemical product killing bacteria effect |
| CN110272968A (en) * | 2019-03-15 | 2019-09-24 | 李文杰 | ATP fluorescence lgCB-lgIBThe method for marking bent method evaluation disposable sanitary articles fungi killing effect |
| CN110819691A (en) * | 2019-12-06 | 2020-02-21 | 石家庄学院 | Method for identifying and evaluating sterilization effect of disinfectant |
| CN111206067A (en) * | 2020-02-18 | 2020-05-29 | 威莱(广州)日用品有限公司 | Method for evaluating wet tissue anticorrosion effect |
| CN112611740A (en) * | 2020-11-27 | 2021-04-06 | 中纺协（北京）检验技术服务有限公司 | Method for detecting antiviral performance of textile |
| CN113933459A (en) * | 2021-09-18 | 2022-01-14 | 中国日用化学研究院有限公司 | Method for testing and evaluating degerming performance of fabric detergent |
| CN113984752A (en) * | 2021-11-02 | 2022-01-28 | 福建省产品质量检验研究院 | Test method for rapidly detecting biological antibacterial performance of food contact material |
| CN114703652A (en) * | 2022-03-23 | 2022-07-05 | 青岛理工大学 | Finishing process of nano antibacterial textile and antibacterial activity evaluation method thereof |
| CN115980321A (en) * | 2022-12-06 | 2023-04-18 | 安徽锦昕医疗用品有限公司 | Isolation suit antibacterial property detection device |
| WO2023116477A1 (en) * | 2021-12-22 | 2023-06-29 | 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) | Method for detecting bacterial filtration efficiency of mask |
| CN117625442A (en) * | 2023-10-27 | 2024-03-01 | 远东正大检验集团有限公司 | Method for detecting odor-causing bacteria strain combination and textile odor resistance |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108485953B (en) * | 2018-04-23 | 2023-08-01 | 上海新型烟草制品研究院有限公司 | Bacteria culture device and method for testing antibacterial performance of buccal cigarettes |
| CN111321196A (en) * | 2020-02-21 | 2020-06-23 | 浙江方圆检测集团股份有限公司 | Escherichia coli suspension culture medium for detecting antibacterial performance by using film pasting method and preparation method thereof |
| CN113430245A (en) * | 2021-08-03 | 2021-09-24 | 广州百仕肽生物科技有限公司 | Quantitative determination method for iturin antibacterial activity |
-
2016
- 2016-07-26 CN CN201610592991.6A patent/CN106191204A/en active Pending
- 2016-11-14 WO PCT/CN2016/105675 patent/WO2018018784A1/en active Application Filing
Non-Patent Citations (1)
| Title |
|---|
| 深圳市北岳海威化工有限公司等: "《中华人民共和国国家标准 GB/T 20944.3-2008》", 29 April 2008 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109402216A (en) * | 2017-08-17 | 2019-03-01 | 宝洁公司 | Method for demonstrating the efficacy against bacteria of toothbrush bristle |
| CN109207554B (en) * | 2018-09-06 | 2021-12-21 | 西安开米股份有限公司 | Method for rapidly detecting bacteriostatic effect of daily chemical product by using TTC agar culture medium |
| CN109207554A (en) * | 2018-09-06 | 2019-01-15 | 西安开米股份有限公司 | The method for quickly detecting daily chemical products fungistatic effect with TTC agar medium |
| CN109609590A (en) * | 2018-12-21 | 2019-04-12 | 扬州倍加洁日化有限公司 | A kind of anti-microbial property test method of wet tissue product |
| CN110272969A (en) * | 2019-03-15 | 2019-09-24 | 李文杰 | ATP bioluminescence lgCA-lgIAThe method of calibration curve method evaluation chemical product killing bacteria effect |
| CN110272968A (en) * | 2019-03-15 | 2019-09-24 | 李文杰 | ATP fluorescence lgCB-lgIBThe method for marking bent method evaluation disposable sanitary articles fungi killing effect |
| CN109762873A (en) * | 2019-03-27 | 2019-05-17 | 苏州纳工坊健康科技有限公司 | A kind of fungicide antibacterial effect detection method and fungicide storing unit |
| CN110819691A (en) * | 2019-12-06 | 2020-02-21 | 石家庄学院 | Method for identifying and evaluating sterilization effect of disinfectant |
| CN111206067A (en) * | 2020-02-18 | 2020-05-29 | 威莱(广州)日用品有限公司 | Method for evaluating wet tissue anticorrosion effect |
| CN112611740A (en) * | 2020-11-27 | 2021-04-06 | 中纺协（北京）检验技术服务有限公司 | Method for detecting antiviral performance of textile |
| CN113933459A (en) * | 2021-09-18 | 2022-01-14 | 中国日用化学研究院有限公司 | Method for testing and evaluating degerming performance of fabric detergent |
| CN113984752A (en) * | 2021-11-02 | 2022-01-28 | 福建省产品质量检验研究院 | Test method for rapidly detecting biological antibacterial performance of food contact material |
| WO2023116477A1 (en) * | 2021-12-22 | 2023-06-29 | 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) | Method for detecting bacterial filtration efficiency of mask |
| CN114703652A (en) * | 2022-03-23 | 2022-07-05 | 青岛理工大学 | Finishing process of nano antibacterial textile and antibacterial activity evaluation method thereof |
| CN115980321A (en) * | 2022-12-06 | 2023-04-18 | 安徽锦昕医疗用品有限公司 | Isolation suit antibacterial property detection device |
| CN117625442A (en) * | 2023-10-27 | 2024-03-01 | 远东正大检验集团有限公司 | Method for detecting odor-causing bacteria strain combination and textile odor resistance |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018018784A1 (en) | 2018-02-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106191204A (en) | A kind of method of testing of probiotic bacteria anti-bacterial household antibacterial textile performance | |
| CN102505467B (en) | A kind of dressing liquid for antibacterial fabric and preparation method thereof | |
| CN104140937B (en) | Lactobacillus plantarum KLDS1001 and application of lactobacillus plantarum KLDS1001 to inhibition of streptococcus mutans | |
| Chen et al. | Factors affecting the susceptibility of Staphylococcus aureus CCRC 12657 to water soluble lactose chitosan derivative | |
| HRP950533A2 (en) | Defined medium ompc fermentation process | |
| CN106400310A (en) | Nano-fiber anti-microbial fruit-vegetable preservative film with porous structure, and preparation method and application | |
| CN103011945A (en) | Lyophyllum decastes solid strain medium formula and strain production method | |
| Cao et al. | Construction and functional assessment of zein thin film incorporating spindle-like ZnO crystals | |
| El Kadri et al. | The antimicrobial efficacy of remote cold atmospheric plasma effluent against single and mixed bacterial biofilms of varying age | |
| CN104313118A (en) | Antibacterial efficacy detection method of diatom antibacterial material | |
| CN104762350B (en) | Biological fermentation bran with antibacterial effect and preparation method and application thereof | |
| CN104706594B (en) | Preparation method of polyprenol microemulsion with antibacterial activity | |
| Chotigarpa et al. | Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli | |
| Jung et al. | Evaluation of the probiotic potential of Bacillus polyfermenticus CJ6 isolated from Meju, a Korean soybean fermentation starter. | |
| CN107760638A (en) | It is a kind of directly to prepare the method and Bdellovibrio culture medium of fresh Bdellovibrio in the using area of Bdellovibrio | |
| CN108795803A (en) | Can efficient degradation malachite green drug degradation bacteria and its application | |
| CN109125399A (en) | The antibacterial action of stephania tetrandra extract | |
| CN106720308A (en) | A kind of method for disinfection of Cronobacter sakazakii and application | |
| Soares et al. | Growth of enterotoxin producing Bacillus cereus in meat substrate at 10ºC and 30ºC | |
| Hwang et al. | Distribution of six exotoxin genes and production of L2-HBL and nheA proteins in six Bacillus cereus isolates from infant formula and produce | |
| CN102154440A (en) | Method for removing residual disinfectant left after disinfection by capturing with magnetic beads | |
| Redhead et al. | Acquisition of iron from transferrin by Bordetella pertussis | |
| Olukemi et al. | Bacterial contamination associated with retail chicken carcasses in Osogbo, Nigeria | |
| CN106822077A (en) | A kind of compound acidulant and its application | |
| CN102582866A (en) | Two-time sterilization, aseptic production and nutrient adding technology for home textiles and clothing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161207 |