CN106190775A - Foetal DNA Non-invasive detection equipment - Google Patents
Foetal DNA Non-invasive detection equipment Download PDFInfo
- Publication number
- CN106190775A CN106190775A CN201610668531.7A CN201610668531A CN106190775A CN 106190775 A CN106190775 A CN 106190775A CN 201610668531 A CN201610668531 A CN 201610668531A CN 106190775 A CN106190775 A CN 106190775A
- Authority
- CN
- China
- Prior art keywords
- dna
- plasma
- detection equipment
- invasive detection
- collection terminal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Abstract
The present invention provides a kind of foetal DNA Non-invasive detection equipment, relate to field of medical device, including Maternal plasma collection terminal, free fetal dna enriching section and DNA sequencing part, described Maternal plasma collection terminal is kept in container by the blood plasma of sampled plasma needle tubing, connection sampled plasma needle tubing and blood plasma attemperator is constituted, and described fetal DNA enrichment part uses cffDNA efficiently concentrating method and cffD NA specific isolation method.By gathering Maternal plasma detection, the equipment of the present invention can judge that fetus suffers from the risk of chromosomal disorders.
Description
Technical field
The present invention relates to field of medical device, particularly relate to a kind of foetal DNA Non-invasive detection equipment.
Background technology
Chromosomal disorders are the modal genopathies of the mankind.Composing type (constitutional) chromosomal disorders occur
Rate accounts for more than the 50% of the relevant fatality rate of miscarriage at first trimenon of gestation, accounts in utero or about the 5% of perinatal mortality.
Additionally the life birth child of at least 0.5% has the composing type chromosomal abnormality relevant to intelligence and/or physical disability.So sentencing in advance
Disconnected fetus suffers from the key that the risk of chromosomal disorders is prenatal and postnatal care.
Summary of the invention
It is an object of the invention to provide a kind of foetal DNA Non-invasive detection equipment, to solve above-mentioned technical problem.
The technical problem to be solved realizes by the following technical solutions:
Foetal DNA Non-invasive detection equipment, it is characterised in that: include Maternal plasma collection terminal, free fetal dna enriching section
With DNA sequencing part, described Maternal plasma collection terminal is kept in container by the blood plasma of sampled plasma needle tubing, connection sampled plasma needle tubing
Constituting with blood plasma attemperator, described fetal DNA enrichment part uses cffDNA efficiently concentrating method and cffD-NA specific isolation
Method.
Described cffDNA efficiently concentrating method, according to the difference of DNA molecular fragment length, specific enrichment fragment length is less than
300bp。
Described DNA sequencing part uses up-to-date DNA sequencing technology.
Maternal plasma collection terminal gathers the plasma sample of parent, by foetal DNA free in enrichment Maternal plasma, and adopts
By a new generation's DNA sequencing technology, it is analyzed, it is judged that fetus suffers from the risk of chromosomal disorders.
The invention has the beneficial effects as follows:
By gathering Maternal plasma detection, the equipment of the present invention can judge that fetus suffers from the risk of chromosomal disorders.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below knot
Close specific embodiment, the present invention is expanded on further, but following embodiment is only the preferred embodiments of the present invention, and not all.
Based on the embodiment in embodiment, it is real that those skilled in the art are obtained other on the premise of not making creative work
Execute example, broadly fall into protection scope of the present invention.
Embodiment 1
Foetal DNA Non-invasive detection equipment, including Maternal plasma collection terminal, free fetal dna enriching section and DNA sequencing portion
Point, described Maternal plasma collection terminal is kept in container and blood plasma insulation by the blood plasma of sampled plasma needle tubing, connection sampled plasma needle tubing
Device is constituted, and described fetal DNA enrichment part uses cffDNA efficiently concentrating method and cffD-NA specific isolation method.
Described cffDNA efficiently concentrating method, according to the difference of DNA molecular fragment length, specific enrichment fragment length is less than
300bp。
Described DNA sequencing part uses up-to-date DNA sequencing technology.
Maternal plasma collection terminal gathers the plasma sample of parent, by foetal DNA free in enrichment Maternal plasma, and adopts
By a new generation's DNA sequencing technology, it is analyzed, it is judged that fetus suffers from the risk of chromosomal disorders.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.The technology of the industry
The personnel only present invention it should be appreciated that the present invention is not restricted to the described embodiments, described in above-described embodiment and description
Preference, be not intended to limit the present invention, without departing from the spirit and scope of the present invention, the present invention also has various
Changes and improvements, these changes and improvements both fall within scope of the claimed invention.Claimed scope is by institute
Attached claims and equivalent thereof define.
Claims (3)
1. a foetal DNA Non-invasive detection equipment, it is characterised in that: include Maternal plasma collection terminal, free fetal dna enrichment portion
Dividing and DNA sequencing part, described Maternal plasma collection terminal is held by the blood plasma of sampled plasma needle tubing, connection sampled plasma needle tubing is temporary
Device and blood plasma attemperator are constituted, and described fetal DNA enrichment part uses cffDNA efficiently concentrating method and cffD-NA specificity to divide
From method.
Foetal DNA Non-invasive detection equipment the most according to claim 1, it is characterised in that: described cffDNA efficiently concentrating method,
According to the difference of DNA molecular fragment length, specific enrichment fragment length is less than 300bp.
Foetal DNA Non-invasive detection equipment the most according to claim 1, it is characterised in that: described DNA sequencing part uses
New DNA sequencing technology.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610668531.7A CN106190775A (en) | 2016-08-08 | 2016-08-08 | Foetal DNA Non-invasive detection equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610668531.7A CN106190775A (en) | 2016-08-08 | 2016-08-08 | Foetal DNA Non-invasive detection equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106190775A true CN106190775A (en) | 2016-12-07 |
Family
ID=57522576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610668531.7A Pending CN106190775A (en) | 2016-08-08 | 2016-08-08 | Foetal DNA Non-invasive detection equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106190775A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232771A (en) * | 2014-09-12 | 2014-12-24 | 吉林大学 | Method of selectively amplifying free fetal DNA (deoxyribonucleic acid) in maternal plasma |
| CN104232777A (en) * | 2014-09-19 | 2014-12-24 | 天津华大基因科技有限公司 | Method and device for simultaneously determining fetal nucleic acid content and aneuploidy of chromosome |
| CN104830986A (en) * | 2015-05-07 | 2015-08-12 | 深圳承启生物科技有限公司 | Method, apparatus, and system for detecting fetus gene information |
| CN104862301A (en) * | 2014-02-21 | 2015-08-26 | 广州康睿生物医药科技有限公司 | Method for separating and enriching free fetus DNA from maternal blood plasma |
-
2016
- 2016-08-08 CN CN201610668531.7A patent/CN106190775A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862301A (en) * | 2014-02-21 | 2015-08-26 | 广州康睿生物医药科技有限公司 | Method for separating and enriching free fetus DNA from maternal blood plasma |
| CN104232771A (en) * | 2014-09-12 | 2014-12-24 | 吉林大学 | Method of selectively amplifying free fetal DNA (deoxyribonucleic acid) in maternal plasma |
| CN104232777A (en) * | 2014-09-19 | 2014-12-24 | 天津华大基因科技有限公司 | Method and device for simultaneously determining fetal nucleic acid content and aneuploidy of chromosome |
| CN104830986A (en) * | 2015-05-07 | 2015-08-12 | 深圳承启生物科技有限公司 | Method, apparatus, and system for detecting fetus gene information |
Non-Patent Citations (2)
| Title |
|---|
| 杨麒巍等: "孕妇外周血中游离胎儿ＤＮＡ富集与分离方法及在无创性产前诊断中的应用", 《中国实验诊断学》 * |
| 赵馨等: "第二代测序技术与无创产前诊断", 《分子诊断与治疗杂志》 * |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161207 |