CN106188344B - A kind of extracting method of high-purity chitin in high molecular weight - Google Patents
A kind of extracting method of high-purity chitin in high molecular weight Download PDFInfo
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- 229920002101 Chitin Polymers 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000000047 product Substances 0.000 claims abstract description 29
- 238000001914 filtration Methods 0.000 claims abstract description 17
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 15
- 241000238631 Hexapoda Species 0.000 claims abstract description 15
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 15
- 239000011575 calcium Substances 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 15
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 15
- 239000002253 acid Substances 0.000 claims abstract description 14
- 239000012065 filter cake Substances 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 238000005406 washing Methods 0.000 claims abstract description 13
- 230000007935 neutral effect Effects 0.000 claims abstract description 12
- 150000003839 salts Chemical class 0.000 claims abstract description 12
- 238000003756 stirring Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 239000012046 mixed solvent Substances 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 9
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 230000029087 digestion Effects 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims abstract description 6
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 claims description 14
- 229940088598 enzyme Drugs 0.000 claims description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 8
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Natural products OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- KBIWNQVZKHSHTI-UHFFFAOYSA-N 4-n,4-n-dimethylbenzene-1,4-diamine;oxalic acid Chemical compound OC(=O)C(O)=O.CN(C)C1=CC=C(N)C=C1 KBIWNQVZKHSHTI-UHFFFAOYSA-N 0.000 claims description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 4
- 239000007832 Na2SO4 Substances 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 3
- 102000004882 Lipase Human genes 0.000 claims description 2
- 108090001060 Lipase Proteins 0.000 claims description 2
- 239000004367 Lipase Substances 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 2
- 235000019421 lipase Nutrition 0.000 claims description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229940055729 papain Drugs 0.000 claims description 2
- 239000001103 potassium chloride Substances 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 210000000496 pancreas Anatomy 0.000 claims 1
- 235000019419 proteases Nutrition 0.000 claims 1
- 230000002378 acidificating effect Effects 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000002351 wastewater Substances 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 2
- 206010039509 Scab Diseases 0.000 description 10
- 239000000243 solution Substances 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
- 238000011084 recovery Methods 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 150000003222 pyridines Chemical class 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 125000002091 cationic group Chemical group 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229910052738 indium Inorganic materials 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- NUHNZPDGBDHDFM-BOIUCGLESA-N O=CC[C@@H](O)[C@H](O)[C@H](O)CO.C(C)(=O)NC=1C(=C(C=CC1)[As](O)(O)=O)O Chemical compound O=CC[C@@H](O)[C@H](O)[C@H](O)CO.C(C)(=O)NC=1C(=C(C=CC1)[As](O)(O)=O)O NUHNZPDGBDHDFM-BOIUCGLESA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000013777 protein digestion Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The present invention provides a kind of extracting methods of high-purity chitin in high molecular weight, include the following steps：(1) it will be placed in diluted acid in 30 50 DEG C of digestions 46 hours, filtering takes filter cake washing and drying to obtain calcium depleted product A after insect crust crushes；(2) calcium depleted product A, complex enzyme and water are placed in reaction vessel, control reaction solution PH is 7.5 8.5, and after 40 55 DEG C of stirrings hydrolyze 23 hours, filtering takes filter cake washing and drying that must take off protein product B；(3) de- protein product B is placed in the mixed solvent, and neutral salt is added, after 60 80 DEG C of stirring and dissolvings 13 hours, filtering, filtrate removes neutral salt through being dried under reduced pressure removal solvent, then through washing, it is dry needed for chitin.The chitin extraction technology of the present invention is simple, and equipment is conventional, acidic and alkaline waste water low output, meets the requirement of environmentally protective production, the purity of gained chitin is high, molecular weight is high, ash content is low.
Description
Technical field
The invention belongs to biotechnologies, and in particular to a kind of extracting method of chitin, more particularly to it is a kind of high-purity
Spend the extracting method of chitin in high molecular weight.
Background technology
Chitin also known as chitin, chitin etc. are many lower animals especially arthropods such as shrimp, crab, insect etc.
The important component (containing about 10-30%) of shell, exists in rudimentary plant such as Homonemeae and the cell wall of fungi, it is by 2-
Natural polymers of the basic unit of acetylaminohydroxyphenylarsonic acid 2-deoxy-D-glucose through β (1 → 4) glucosides key connection, are so far
The cationic animal origin and unique alkaline polysaccharide uniquely found until the present.Chitin and its derivative have many unique
Function and characteristic are widely applied in fields such as environmental protection, food, medicine, making functional material, agriculture and forestry, light and textile industries,
With huge economic value and it is wide answer rigid foreground, as what chitin and its derivatives chemical were studied deepens continuously, first
Shell element industry will be flourished in this century.
The source of chitin is mainly shrimp, crab shell and insect crust at present, and wherein chitin content is higher in insect crust,
Calcium and content of beary metal are low, and impurity is few, and purity is high, high-quality, are consumed less to water, acid, alkali in extraction process, and production cost is low,
Therefore, insect chitin is better than shrimp, crab chitin.
Chinese patent CN104031176A provides a kind of flyblow chitin extracting method, and fly maggot shell is stirred using hydrochloric acid
After mixing immersion centrifugation, take solids that sodium hydrate aqueous solution is added, 80-90 DEG C of water-bath 20min takes solids again after centrifugation,
Chitin is obtained after washing, dries pulverizing, it is various using the method step, and acidic and alkaline waste water is more, the requirement to equipment is high, gained first
Shell element purity and molecular weight be not high.
Invention content
It is easy to operate, equipment is conventional, gained the present invention provides a kind of extracting method of high-purity chitin in high molecular weight
The molecular weight of chitin is high, purity is high, ash content is few.
The technical proposal for solving the technical problem of the invention is：
A kind of extracting method of high-purity chitin in high molecular weight, includes the following steps：
(1) deliming：After insect crust is crushed, it is placed in the diluted acid that mass concentration is 10-20% in 30-50 DEG C of digestion 4-
It 6 hours, filters, it is dry that calcium depleted product A is for use after taking filter cake to wash 3-5 times, a large amount of calcareous, utilization is contained in insect shell
Diluted acid is dissolved removal, and raw material is further decomposed.
(2) albumen is taken off：Calcium depleted product A, complex enzyme and water are placed in reaction vessel, control reaction solution PH is 7.5-
8.5, after 40-55 DEG C of stirring hydrolyzes 2-3 hours, filtering takes filter cake washing and drying that must take off protein product B for use；It is described compound
Enzyme is the mixture of trypsase, papain and alkaline lipase, and the mass ratio of three is 1：1：2.It will using complex enzyme
Protein digestion in filter cake A is simultaneously soluble in water, and reaction condition is mild, using the complex enzyme of the formula to albumen in insect crust
The enzymolysis efficiency higher of matter, hydrolysis result are more preferable.The chitin of high quality can be obtained by taking off albumen compared to sodium hydroxide, and will not
The molecular chain rupture for causing chitin, to reduce its molecular weight.
(3) it purifies：De- protein product B is placed in the mixed solvent, and neutral salt is added, in 60-80 DEG C of stirring and dissolving 1-
After 3 hours, filtering, filtrate removes neutral salt through being dried under reduced pressure removal solvent, then through washing, it is dry needed for chitin；It is described
Mixed solvent is the mixture of caproic acid, ethanedioic acid and pyridine, and the mass ratio of three is 1：0.8：0.5.By filter cake B through mixed solvent
Dissolving is concentrated to give the chitin product of high-purity high molecular weight again.There is stable cyclic structure and big in chitin macromolecular
There are strong hydrogen bond action between molecule, its solubility property is made to be deteriorated, not soluble in water, diluted acid, diluted alkaline and general organic molten
In agent.Chitin can dissolve in the strong acid such as the concentrated sulfuric acid, hydrochloric acid, nitric acid and 85% phosphoric acid, but at the same time can occur violent
Degradation, makes relative molecular mass be substantially reduced.The mixed solvent of caproic acid of the present invention, ethanedioic acid and pyridine, to crust
Plain dissolution rate is high, while will not reduce its molecular weight.It is to increase the ionic strength of reaction solution, utilize crust that neutral salt, which is added,
The cationic characteristic of element enhances its dissolution rate in the mixed solvent.
Preferably, diluted acid described in step (1) is hydrochloric acid, sulfuric acid or phosphoric acid.
Preferably, diluted acid quality described in step (1) is 20-30 times of insect crust quality.
Preferably, the mass ratio of calcium depleted product A, complex enzyme and water described in step (2) are 1：0.05-0.08：10-
30。
Preferably, the mass ratio for taking off protein product B, mixed solvent and neutral salt described in step (3) is 1：15-20：
0.1-0.3。
Preferably, the neutral salt is NaCl, KCl, Na2SO4、MgCl2Or MgSO4。
Beneficial effects of the present invention are：
1, using the protein and fat in complex enzyme for hydrolyzing insect shell, hydrolysising condition is mild, and enzymolysis efficiency is high, and not
Chitin molecule amount can be reduced.
2, using the mixed solvent of caproic acid, ethanedioic acid and pyridine combination and neutral salt collaboration dissolving chitin, to chitin
Dissolution rate is high, helps to improve the chitin rate of recovery and purity, while will not reduce its molecular weight.
3, chitin extraction technology of the invention is simple, and equipment is conventional, and acidic and alkaline waste water low output meets environmentally protective production
Requirement.
Specific implementation mode
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.
Embodiment 1
(1) deliming：After 10g insect crusts are crushed, it is placed in the diluted acid that 200g mass concentrations are 10% in 50 DEG C of digestions 4
Hour, filtering obtains 7.2g calcium depleted products A after taking filter cake to wash 3-5 drying；
(2) albumen is taken off：By 7.2g calcium depleted product A, 0.09g trypsase, 0.09g papains, 0.18g alkalinity fat
Fat enzyme and 144g water are placed in three-necked flask, and control reaction solution PH is 7.5, and after 40 DEG C of stirrings hydrolyze 3 hours, filtering takes filter
Cake washing and drying obtains 4.3g and takes off protein product B；
(3) it purifies：4.3g is taken off in protein product B, 28g caproic acid, 22g ethanedioic acids, 14g pyridines and 0.43gNaCl, in
60 DEG C of stirring and dissolvings are after 3 hours, filtering, and filtrate removes NaCl through being dried under reduced pressure removal solvent, then through washing, dry 3.2g first
Shell element, HPLC purity are 92.3%, the rate of recovery 29.54%, ash content 0.8%.
Embodiment 2
(1) deliming：After 10g insect crusts are crushed, it is placed in the diluted acid that 250g mass concentrations are 15% in 30 DEG C of digestions 6
Hour, filtering obtains 7.8g calcium depleted products A after taking filter cake to wash 3-5 drying；
(2) albumen is taken off：By 7.8g calcium depleted product A, 0.12g trypsase, 0.12g papains, 0.24g alkalinity fat
Fat enzyme and 78g water are placed in three-necked flask, and control reaction solution PH is 8, and after 50 DEG C of stirrings hydrolyze 2.5 hours, filtering takes filter cake
Washing and drying obtains 5.1g and takes off protein product B；
(3) it purifies：5.1g is taken off into protein product B, 40g caproic acid, 32g ethanedioic acids, 20g pyridines and 1g MgSO4In, in 70
DEG C stirring and dissolving is after 2 hours, filtering, and filtrate removes MgSO through being dried under reduced pressure removal solvent, then through washing4, dry 3.8g first
Shell element, HPLC purity are 93.8%, the rate of recovery 35.6%, ash content 0.78%.
Embodiment 3
(1) deliming：After 10g insect crusts are crushed, it is placed in the diluted acid that 300g mass concentrations are 20% in 40 DEG C of digestions 5
Hour, filtering obtains 6.9g calcium depleted products A after taking filter cake to wash 3-5 drying；
(2) albumen is taken off：By 6.9g calcium depleted product A, 0.14g trypsase, 0.14g papains, 0.28g alkalinity fat
Fat enzyme and water are placed in three-necked flask, and control reaction solution PH is 8.5, and after 55 DEG C of stirrings hydrolyze 2 hours, filtering takes filter cake water
It washes dry that 4.6g takes off protein product B；
(3) it purifies：4.6g is taken off into protein product B, 40g caproic acid, 32g ethanedioic acids, 20g pyridines and 1.4g Na2SO4In, in
80 DEG C of stirring and dissolvings are after 1 hour, filtering, and filtrate removes Na through being dried under reduced pressure removal solvent, then through washing2SO4, dry 3.5g
Chitin, HPLC purity are 93.1%, the rate of recovery 32.6%, ash content 0.72%.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, on the premise of not exceeding the technical scheme recorded in the claims also other variations and modifications.
Claims (6)
1. a kind of extracting method of high-purity chitin in high molecular weight, which is characterized in that include the following steps：
(1) deliming：After insect crust is crushed, it is placed in small in 30-50 DEG C of digestion 4-6 in the diluted acid that mass concentration is 10-20%
When, filtering is dry that calcium depleted product A is for use after taking filter cake to wash 3-5 times；
(2) albumen is taken off：Calcium depleted product A, complex enzyme and water are placed in reaction vessel, control reaction solution pH is 7.5-8.5, in
After 40-55 DEG C of stirring hydrolyzes 2-3 hours, filtering takes filter cake washing and drying that must take off protein product B for use；The complex enzyme is pancreas
The mass ratio of the mixture of protease, papain and alkaline lipase, three is 1：1：2；
(3) it purifies：De- protein product B is placed in the mixed solvent, and neutral salt is added, it is small in 60-80 DEG C of stirring and dissolving 1-3
Shi Hou, filtering, filtrate remove neutral salt through being dried under reduced pressure removal solvent, then through washing, it is dry needed for chitin；The mixing
Solvent is the mixture of caproic acid, ethanedioic acid and pyridine, and the mass ratio of three is 1：0.8：0.5.
2. high-purity chitin in high molecular weight extracting method as described in claim 1, which is characterized in that described in step (1)
Diluted acid is hydrochloric acid, sulfuric acid or phosphoric acid.
3. high-purity chitin in high molecular weight extracting method as described in claim 1, which is characterized in that described in step (1)
Diluted acid quality is 20-30 times of insect crust quality.
4. high-purity chitin in high molecular weight extracting method as described in claim 1, which is characterized in that described in step (2)
The mass ratio of calcium depleted product A, complex enzyme and water are 1：0.05-0.08：10-30.
5. high-purity chitin in high molecular weight extracting method as described in claim 1, which is characterized in that described in step (3)
The mass ratio of de- protein product B, mixed solvent and neutral salt are 1：15-20：0.1-0.3.
6. the high-purity chitin in high molecular weight extracting method as described in claim 1 or 5, which is characterized in that the neutral salt
For NaCl, KCl, Na2SO4、MgCl2Or MgSO4。
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| CN106977623A (en) * | 2017-04-13 | 2017-07-25 | 广东人为峰健康管理有限公司 | A kind of method of chitin extraction in fly maggot from drosophila |
| FR3075204B1 (en) * | 2017-12-15 | 2020-11-20 | Ynsect | CHITINE AND PROCESS FOR OBTAINING CHITINE AND / OR CHITOSAN BY CHEMICAL MEANS |
| CN110183510B (en) * | 2019-05-28 | 2023-06-23 | 广东省农业科学院蚕业与农产品加工研究所 | Method for separating insect protein, grease and chitin by natural eutectic solvent in one step and application thereof |
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| CN102161714A (en) * | 2011-01-28 | 2011-08-24 | 上海海洋大学 | Process for preparing chitosan and calcium citrate |
| CN102168323A (en) * | 2011-02-24 | 2011-08-31 | 天津工业大学 | Method for preparing chitosan and chitin functional materials by taking ionic liquid as solvent |
| CN103502344A (en) * | 2011-03-23 | 2014-01-08 | 株式会社Kri | Solvent used for dissolving polysaccharide and method for manufacturing molded article and polysaccharide derivative using this solvent |
| CN105418802A (en) * | 2015-12-29 | 2016-03-23 | 广西钦州市绿源天然食品加工有限公司 | Method for extracting chitin from shrimp heads and shrimp shells by utilization of organic acids and combined with acidic proteases |
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| JP4750265B2 (en) * | 2000-11-02 | 2011-08-17 | ダイセル化学工業株式会社 | Chitin derivatives containing fluorine in the substituent |
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