CN106188189A - A kind of nucleotide mother solution desalination and concentration method - Google Patents
A kind of nucleotide mother solution desalination and concentration method Download PDFInfo
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- CN106188189A CN106188189A CN201610520997.2A CN201610520997A CN106188189A CN 106188189 A CN106188189 A CN 106188189A CN 201610520997 A CN201610520997 A CN 201610520997A CN 106188189 A CN106188189 A CN 106188189A
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- nucleotide
- mother solution
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- concentration method
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of nucleotide mother solution desalination and concentration method, specific as follows: nucleotide mother solution is placed in batch can by (1), start delivery pump, after nucleotide mother solution is concentrated 2.5～5 times by membrane module, continue the most continual isopyknic water of amount added in batch can with the instant permeate produced to continue to run with, total amount of water is equal to the 65～75% of above-mentioned nucleotide mother solution volume, and final positive and negative yield is all higher than 99%；The operating pressure of the said equipment is 20～28bar, and temperature is 20～30 DEG C, and average flux is 12～30LMH；(2) rinse the residual liquid in core rod and pipeline with water, then under conditions of 30～40 DEG C, clean 0.4～0.6h with cleanout fluid, can substantially recover core rod flux.
Description
Technical field
The invention belongs to membrane technology field, be specifically related to a kind of nucleotide mother solution desalination and concentration method.
Background technology
Nucleotide be by nucleic acid hydrolysis and through separating further, concentrating, be dried obtained by biological product, almost participate in cell
All biochemical processes.In food service industry, nucleotide, by initial food fragrance adding agent, expands to have raising organism
The functional food additives of immunologic function.In infant food, particularly there is irreplaceable effect, mend in Lac Bovis seu Bubali
After having filled nucleotide, the milk replacer close to human milk can be produced, strengthen baby's resistivity [2-3] to bacterial disease.This
Outward, nucleotide also has a wide range of applications in medicine, agricultural, fine chemistry industry.Therefore to realizing the large-scale production of nucleotide,
There is highly important social benefit and significant economic benefit.
Containing impurity such as a certain amount of phosphate anions in the existing nucleotide hydrolysis liquid produced by edman degradation Edman, at present
Domestic and international commonly used ion exchange resin and the method separating nucleotide of chromatograph, this method can not large-scale production, and price
Expensive.
Summary of the invention
It is an object of the invention to overcome prior art defect, it is provided that a kind of nucleotide mother solution desalination and concentration method.
The concrete technical scheme of the present invention is as follows:
A kind of nucleotide mother solution desalination and concentration method, its device therefor includes passing sequentially through batch can that pipeline is connected, defeated
Sending pump, membrane module, pressure regulator valve and heat exchanger, wherein the core rod of membrane module is 3B01 NF membrane, and this membrane module has a concentrated solution
Outlet and one permeate outlet, concentrated solution outlet is connected with pressure regulator valve by pipeline, the liquid outlet of heat exchanger pass through pipeline and
Batch can is connected,
The method is specific as follows:
(1) nucleotide mother solution is placed in batch can, start delivery pump, by nucleotide mother solution by membrane module concentrate 2.8～
After 3.3 times, continue the most continual isopyknic water of amount added in batch can with the instant permeate produced and continue
Running, total amount of water is equal to the 70% of above-mentioned nucleotide mother solution volume, and final Na clearance is 71～80%, and Cl clearance is
93.2～93.8%, by the content of liquid nucleotide less than 0.05g/L, positive and negative yield is all higher than 99%.；
(2) rinse the residual liquid in core rod and pipeline with water, then clean under conditions of 30～40 DEG C with cleanout fluid
0.4～0.6h, can substantially recover core rod flux.
In a preferred embodiment of the invention, described nucleotide mother solution is nucleotide primary crystallization mother solution (i.e. core
Thuja acid feed liquid debris after crystallization).
It is further preferred that the operating pressure of described equipment is 20～26bar, temperature is 20～26 DEG C, and average flux is
12～13.5LMH.
It is further preferred that described step (1) is: be placed in batch can by nucleotide mother solution, start delivery pump, by nucleotide
Mother solution is concentrated after 2.8～3.3 times by membrane module, continues the most continual add in batch can with generation immediately saturating
The isopyknic water of amount crossing liquid continues to run with, and total amount of water is equal to the 70% of above-mentioned nucleotide mother solution volume, and final Na removes
Rate is 71～80%, and Cl clearance is 93.2～93.8%, by the content of liquid nucleotide less than 0.05g/L, positive and negative yield
It is all higher than 99%.
In a preferred embodiment of the invention, described nucleotide mother solution is nucleotide secondary crystallization mother solution (i.e. core
Crystalline mother solution after thuja acid secondary crystallization is complete).
It is further preferred that the operating pressure of described equipment is 20～28bar, temperature is 23～30 DEG C, and average flux is
25～30LMH.
It is further preferred that described step (1) is: be placed in batch can by nucleotide mother solution, start delivery pump, by nucleotide
Mother solution concentrates 5 times by membrane module, and final anti-yield is more than 99%.
The invention has the beneficial effects as follows: the method for the present invention can effectively remove Na and Cl in nucleotide mother solution, simultaneously
Having the positive and negative yield more than 99%, desalting effect is good, and applicable industrialized production.
Accompanying drawing explanation
Fig. 1 is the structural representation of the equipment of the present invention.
Fig. 2 is the membrane flux curve chart of the embodiment of the present invention 1.
Fig. 3 is the membrane flux curve chart of the embodiment of the present invention 2.
Detailed description of the invention
Combine accompanying drawing below by way of detailed description of the invention technical scheme is further detailed and describes.
Embodiment 1 nucleotide primary crystallization mother solution is stably tested
As it is shown in figure 1, this embodiment device therefor includes passing sequentially through batch can 1 that pipeline is connected, delivery pump 2, film group
Part 3, pressure regulator valve 4 and heat exchanger 5, wherein the core rod of membrane module 3 is 3B01 NF membrane, and this membrane module 3 has a concentrated solution outlet
With a permeate outlet, concentrated solution outlet is connected with pressure regulator valve 4 by pipeline, and the liquid outlet of heat exchanger 5 passes through pipeline and material
Tank 1 is connected,
The method is specific as follows:
(1) nucleotide primary crystallization mother solution is placed in batch can 1, starts delivery pump 2, nucleotide primary crystallization mother solution is led to
Cross after membrane module 3 concentrates 2.5～5 times, continue the most continual interpolation in batch can 1 and the instant permeate produced
Measuring isopyknic water to continue to run with, total amount of water is equal to the 65～75% of above-mentioned nucleotide primary crystallization mother solution volume, finally
Positive and negative yield is all higher than 99%；The operating pressure of the said equipment is 20～28bar, and temperature is 20～30 DEG C, and average flux is 12
～30LMH；The operating pressure of described equipment is 20～26bar, and temperature is 20～26 DEG C, and average flux is 12～13.5LMH；
(2) rinse the residual liquid in core rod and pipeline with water, then clean under conditions of 30～40 DEG C with cleanout fluid
0.4～0.6h, can substantially recover core rod flux.
By nucleotide primary crystallization mother solution according to above-mentioned steps, carry out stable experiment, altogether carry out 3 batches, every batch
Secondary taking a certain amount of nucleotide primary crystallization mother solution and test, operating pressure controls 20-25bar, temperature control 30 DEG C with
In, concentrating about 3 times, amount of water is about 2 times of concentrated solution, finally the clearance of Na can be reached about 80%, cl's
Clearance about 93%, waste liquid nucleotide content is less than 0.05g/L；Positive and negative yield is all higher than 99%；Detailed Experimental data are shown in
Table 1 below, table 2 and Fig. 2:
Table 1: experiment service data
Table 2: experimental test data
Interpretation
Control, in 30 DEG C, to operate under conditions of operating pressure 20-26bar in running temperature, be concentrated into 3 times of left sides
The right side, flux is reduced to about 10LMH, and the 2 times of concentrated solution volumes that start to add water carry out eluting, and liquid waste residues content is less than 0.02g/L,
Positive and negative yield is all higher than 99%；Clearance about 80% to Na, the clearance of cl is about 93%；Film core flux runs relatively
Stable, average flux reaches 12-13.5LMH；Experimentation flux detector changes, and meets film core moving law, in the starting stage
Feed concentration is relatively low, and flux is relatively big, begins to decline with concentrating flux, and along with adding water elution, flux slightly rises, directly
To tending towards stability.
Embodiment 2 nucleotide primary crystallization mother solution is stably tested
As it is shown in figure 1, this embodiment device therefor includes passing sequentially through batch can 1 that pipeline is connected, delivery pump 2, film group
Part 3, pressure regulator valve 4 and heat exchanger 5, wherein the core rod of membrane module 3 is 3B01 NF membrane, and this membrane module 3 has a concentrated solution outlet
With a permeate outlet, concentrated solution outlet is connected with pressure regulator valve 4 by pipeline, and the liquid outlet of heat exchanger 5 passes through pipeline and material
Tank 1 is connected,
The method is specific as follows:
(1) nucleotide secondary crystallization mother solution is placed in batch can 1, starts delivery pump 2, nucleotide secondary crystallization mother solution is led to
Crossing membrane module 3 and concentrate 5 times, final anti-yield is more than 99%；The operating pressure of described equipment is 20～28bar, temperature be 23～
30 DEG C, average flux is 25～30LMH；
(2) rinse the residual liquid in core rod and pipeline with water, then clean under conditions of 30～40 DEG C with cleanout fluid
0.4～0.6h, can substantially recover core rod flux.
Nucleotide secondary crystallization mother liquor concentrations is stably tested and has the most altogether been carried out 2 batches, takes appropriate respectively
Nucleotide secondary crystallization mother solution test, operating pressure controls at 20-28bar, and first batch experimental temperature controls 25
About DEG C, the second batch experimental temperature controls to carry out contrast experiment at about 30 DEG C, and in terms of experimental test data, temperature gets over low yield
Product transmitance is the lowest, and at 25 DEG C of experimental waste liquid content less than 0.02g/L, 30 DEG C of experimental temperature content are less than 0.4g/L；To secondary
De-mother solution can concentrate about 5 times, and average operating flux reaches 25-30LM, and the anti-yield of product is more than 99%.Detailed Experimental data
See table 3 and Fig. 3:
Table 3: experimental data
Interpretation
Controlling, in 30 DEG C, to operate under conditions of operating pressure 20-28bar in running temperature, secondary takes off mother solution can
To concentrate 5-6 times, average flux reaches 25-30LMH；The anti-yield of product yield can reach more than 99%, experimentation flux
Curvilinear motion, meets film core moving law, relatively low at starting stage feed concentration, and flux is relatively big, opens with concentrating flux
Begin to decline.
Those of ordinary skill in the art understand, and when technical scheme changes in following ranges, remain able to
Technique effect to same as the previously described embodiments or close:
A kind of nucleotide mother solution desalination and concentration method, its device therefor includes passing sequentially through batch can that pipeline is connected, defeated
Sending pump, membrane module, pressure regulator valve and heat exchanger, wherein the core rod of membrane module is 3B01 NF membrane, and this membrane module has a concentrated solution
Outlet and one permeate outlet, concentrated solution outlet is connected with pressure regulator valve by pipeline, the liquid outlet of heat exchanger pass through pipeline and
Batch can is connected,
The method is specific as follows:
(1) nucleotide mother solution is placed in batch can, start delivery pump, by nucleotide mother solution by membrane module concentrate 2.8～
After 3.3 times, continue the most continual isopyknic water of amount added in batch can with the instant permeate produced and continue
Running, total amount of water is equal to the 70% of above-mentioned nucleotide mother solution volume, and final Na clearance is 71～80%, and Cl clearance is
93.2～93.8%, by the content of liquid nucleotide less than 0.05g/L, positive and negative yield is all higher than 99%.；
(2) rinse the residual liquid in core rod and pipeline with water, then clean under conditions of 30～40 DEG C with cleanout fluid
0.4～0.6h, can substantially recover core rod flux.
The above, only presently preferred embodiments of the present invention, therefore the scope that the present invention implements can not be limited according to this, i.e.
The equivalence change made according to the scope of the claims of the present invention and description with modify, all should still belong in the range of the present invention contains.
Claims (7)
1. a nucleotide mother solution desalination and concentration method, it is characterised in that: its device therefor includes that passing sequentially through pipeline is connected
Batch can, delivery pump, membrane module, pressure regulator valve and heat exchanger, wherein the core rod of membrane module is 3B01 NF membrane, and this membrane module has
One concentrated solution outlet and permeate outlet, concentrated solution outlet is connected with pressure regulator valve by pipeline, and the liquid outlet of heat exchanger leads to
Cross pipeline to be connected with batch can,
The method is specific as follows:
(1) nucleotide mother solution is placed in batch can, starts delivery pump, nucleotide mother solution is concentrated 2.5～5 times by membrane module
After, continue the most continual isopyknic water of amount added in batch can with the instant permeate produced and continue to run with,
Total amount of water is equal to the 65～75% of above-mentioned nucleotide mother solution volume, and final positive and negative yield is all higher than 99%；The said equipment
Operating pressure is 20～28bar, and temperature is 20～30 DEG C, and average flux is 12～30LMH；
(2) rinse the residual liquid in core rod and pipeline with water, then with cleanout fluid clean under conditions of 30～40 DEG C 0.4～
0.6h, can recover core rod flux substantially.
2. a kind of nucleotide mother solution desalination and concentration method as claimed in claim 1, it is characterised in that: described nucleotide mother solution is
Nucleotide primary crystallization mother solution.
3. a kind of nucleotide mother solution desalination and concentration method as claimed in claim 2, it is characterised in that: the operation pressure of described equipment
Power is 20～26bar, and temperature is 20～26 DEG C, and average flux is 12～13.5LMH.
4. a kind of nucleotide mother solution desalination and concentration method as claimed in claim 3, it is characterised in that: described step (1) is: will
Nucleotide mother solution is placed in batch can, starts delivery pump, after nucleotide mother solution is concentrated 2.8～3.3 times by membrane module, continues root
According to needing the continual isopyknic water of amount added in batch can with the instant permeate produced to continue to run with, total amount of water
Equal to the 70% of above-mentioned nucleotide mother solution volume, final Na clearance is 71～80%, and Cl clearance is 93.2～93.8%, logical
The content crossing liquid nucleotide is less than 0.05g/L, and positive and negative yield is all higher than 99%.
5. a kind of nucleotide mother solution desalination and concentration method as claimed in claim 1, it is characterised in that: described nucleotide mother solution is
Nucleotide secondary crystallization mother solution.
6. a kind of nucleotide mother solution desalination and concentration method as claimed in claim 5, it is characterised in that: the operation pressure of described equipment
Power is 20～28bar, and temperature is 23～30 DEG C, and average flux is 25～30LMH.
7. a kind of nucleotide mother solution desalination and concentration method as claimed in claim 6, it is characterised in that: described step (1) is: will
Nucleotide mother solution is placed in batch can, starts delivery pump, and by membrane module, nucleotide mother solution is concentrated 5 times, and final anti-yield is more than
99%.
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|---|---|---|---|
| CN201610520997.2A CN106188189B (en) | 2016-07-04 | 2016-07-04 | A kind of nucleosides acid mother liquor desalination and concentration method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610520997.2A CN106188189B (en) | 2016-07-04 | 2016-07-04 | A kind of nucleosides acid mother liquor desalination and concentration method |
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| CN106188189A true CN106188189A (en) | 2016-12-07 |
| CN106188189B CN106188189B (en) | 2019-04-16 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111704637A (en) * | 2020-06-28 | 2020-09-25 | 南京工业大学 | Method for refining nucleotide by adopting membrane distillation crystallization |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1884523A (en) * | 2006-06-13 | 2006-12-27 | 南京工业大学 | Method for separating and preparing 5'-nucleotide using emulated moving bed |
| CN101418327A (en) * | 2008-11-21 | 2009-04-29 | 大连珍奥生物技术股份有限公司 | The new process of production of high purity 5 ' Nucleotide |
| CN103127833A (en) * | 2013-02-05 | 2013-06-05 | 山东兆光色谱分离技术有限公司 | Nanofiltration membrane system applied to fermented liquid purification |
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2016
- 2016-07-04 CN CN201610520997.2A patent/CN106188189B/en active Active
Patent Citations (3)
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|---|---|---|---|---|
| CN1884523A (en) * | 2006-06-13 | 2006-12-27 | 南京工业大学 | Method for separating and preparing 5'-nucleotide using emulated moving bed |
| CN101418327A (en) * | 2008-11-21 | 2009-04-29 | 大连珍奥生物技术股份有限公司 | The new process of production of high purity 5 ' Nucleotide |
| CN103127833A (en) * | 2013-02-05 | 2013-06-05 | 山东兆光色谱分离技术有限公司 | Nanofiltration membrane system applied to fermented liquid purification |
Non-Patent Citations (1)
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| 姚志春: "纳滤膜技术在核苷酸浓缩中的应用", 《甘肃科学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111704637A (en) * | 2020-06-28 | 2020-09-25 | 南京工业大学 | Method for refining nucleotide by adopting membrane distillation crystallization |
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