CN106179782A - For the orifice plate of centrifugally operated and centrifuge head and centrifuge - Google Patents
For the orifice plate of centrifugally operated and centrifuge head and centrifuge Download PDFInfo
- Publication number
- CN106179782A CN106179782A CN201510229249.4A CN201510229249A CN106179782A CN 106179782 A CN106179782 A CN 106179782A CN 201510229249 A CN201510229249 A CN 201510229249A CN 106179782 A CN106179782 A CN 106179782A
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- China
- Prior art keywords
- centrifuge
- bar
- orifice plate
- head
- base
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Abstract
The invention discloses a kind of orifice plate for centrifugally operated, it includes base and multiple placement bar, and this base offers multiple groove, and each placement bar can releasably be arranged in the groove of correspondence, and this placement bar offers the multiple putting holes for placing centrifuge tube.In above-mentioned orifice plate, when being centrifuged operation, multiple centrifuge tubes can be placed in one and place on bar, it is placed on centrifuge head after the placement bar being placed with multiple centrifuge tube is taken out from base, therefore, once multiple centrifuge tubes can be centrifuged operation, it is to avoid the uninteresting and substantial amounts of duplication of labour, and then improve efficiency of research and development.Invention additionally discloses a kind of centrifuge head and a kind of centrifuge.
Description
Technical field
The present invention relates to biology field, more specifically, relate to a kind of hole for centrifugally operated
Plate and a kind of centrifuge head and a kind of centrifuge.
Background technology
Current biological development has come into big data age.High throughput sequencing technologies, unicellular grinds
Studying carefully etc. needs the substantial amounts of sample of single treatment.The sequencing library construction work of these great amount of samples is to laboratory
Research staff causes huge pressure, is badly in need of a series of method for simplifying to improve scientific research efficiency.
Orifice plate is a kind of consumptive material common in laboratory, typically has 96 holes, is used for placing centrifuge tube,
As PCR (Polymerase Chain Reaction, polymerase chain reaction) manages.Daily
Operation such as build storehouse operation in, be often loaded once will be centrifuged operation sample or reagent are centrifuged at the bottom of pipe
Just can carry out next step operation.Substantial amounts of PCR pipe is taken off one by one centrifugal latter another one
Putting back to, be sometimes also noted that the order of placement, such operation is the most uninteresting and substantial amounts of repetition labor
The dynamic efficiency of research and development greatly reducing scientific research personnel.
For the time used during shortening orifice plate centrifugally operated, improve scientific research efficiency, compel at present to be essential
Want the while of a kind of convenient operation, to save the orifice plate of experimental period and centrifuge head and centrifuge.
Summary of the invention
It is contemplated that at least solve one of technical problem present in prior art.To this end, the present invention needs
A kind of orifice plate for centrifugally operated and a kind of centrifuge head and a kind of centrifuge are provided.
A kind of orifice plate for centrifugally operated, including base and multiple placement bar, this base offers multiple
Groove, each placement bar can releasably be arranged in the groove of correspondence, and this placement bar offers for putting
Put multiple putting holes of centrifuge tube.
In above-mentioned orifice plate, when being centrifuged operation, multiple centrifuge tubes can be placed in one and place on bar,
It is placed on centrifuge head after the placement bar being placed with multiple centrifuge tube is taken out from base, therefore,
Once multiple centrifuge tubes can be centrifuged operation, it is to avoid the uninteresting and substantial amounts of duplication of labour, Jin Erti
High efficiency of research and development.
In one embodiment, the opposing both sides of each placement bar are provided with bearing and leaning portion, and this bearing and leaning portion is supported
Surface by this base.
In one embodiment, this orifice plate also includes lid, and this lid can releasably cover and be located at this end
On seat and the plurality of placement bar.
A kind of centrifuge head, including support bar and supporting part, this support bar is fixed on the end of this supporting part
Face, this supporting part includes support arm, and this support arm offers bar shape trench hole, and this bar shape trench hole is used for placing
The placement bar of orifice plate.
In one embodiment, this supporting part includes base plate, and the opposing both sides of this base plate are provided with two
This support arm, this support bar is fixed on the bottom surface of this base plate.
In one embodiment, these two support arms are symmetrically disposed on this base plate.
Above-mentioned centrifuge head, when being centrifuged operation, multiple centrifuge tubes can be placed in one and place bar
On, it is placed on bar shape trench hole after the placement bar being placed with multiple centrifuge tube is taken out from base, therefore,
Once multiple centrifuge tubes can be centrifuged operation, it is to avoid the uninteresting and substantial amounts of duplication of labour, Jin Erti
High efficiency of research and development.
A kind of centrifuge, including centrifuge fuselage and centrifuge head, this centrifuge head be arranged on this from
On scheming fuselage, this centrifuge head includes support bar and supporting part, and this support bar is fixed on this supporting part
Bottom surface, this supporting part includes support arm, and this support arm offers bar shape trench hole, and this bar shape trench hole is used for
Place the placement bar of orifice plate.
In one embodiment, this supporting part includes base plate, and the opposing both sides of this base plate are provided with two
This support arm, this support bar is fixed on the bottom surface of this base plate.
In one embodiment, these two support arms are symmetrically disposed on this base plate.
In above-mentioned centrifuge, when being centrifuged operation, multiple centrifuge tubes can be placed in one and place on bar,
It is placed on bar shape trench hole after the placement bar being placed with multiple centrifuge tube is taken out from base, therefore, can
Once multiple centrifuge tubes are centrifuged operation, it is to avoid the uninteresting and substantial amounts of duplication of labour, and then improve
Efficiency of research and development.
The additional aspect of the present invention and advantage will part be given in the following description, and part is by from following
Description becomes obvious, or recognized by the practice of the present invention.
Accompanying drawing explanation
Embodiment is retouched by the above-mentioned and/or additional aspect of the present invention and advantage from combining accompanying drawings below
Will be apparent from easy to understand in stating, wherein:
Fig. 1 is the schematic perspective view of the orifice plate of better embodiment of the present invention；
Fig. 2 is the decomposed schematic diagram of the orifice plate of better embodiment of the present invention；
Fig. 3 is the decomposing schematic representation of the orifice plate omission lid of better embodiment of the present invention；
Fig. 4 is the schematic perspective view of the centrifuge loading orifice plate of better embodiment of the present invention；And
Fig. 5 is the schematic perspective view of the centrifuge dismounting orifice plate of better embodiment of the present invention.
Detailed description of the invention
Embodiments of the present invention are described below in detail, and the example of this embodiment is shown in the drawings, its
In the most same or similar label represent same or similar element or there is same or like merit
The element of energy.The embodiment described below with reference to accompanying drawing is exemplary, is only used for explaining this
Bright, and be not considered as limiting the invention.
In describing the invention, it is to be understood that term " center ", " longitudinally ", " laterally ",
" length ", " width ", " thickness ", " going up ", " under ", " front ", " afterwards ", " left ",
" right ", " vertically ", " level ", " top ", " end ", " interior ", " outward ", " up time
The orientation of the instructions such as pin ", " counterclockwise " or position relationship be based on orientation shown in the drawings or pass, position
System, be for only for ease of describe the present invention and simplify describe rather than instruction or hint indication device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to this
The restriction of invention.Additionally, term " first ", " second " are only used for describing purpose, and it is not understood that
For indicating or imply relative importance or the implicit quantity indicating indicated technical characteristic.Thus, limit
Surely have " first ", the feature of " second " can express or implicitly include one or more should
Feature.In describing the invention, " multiple " are meant that two or more, unless otherwise bright
The most concrete restriction.
In describing the invention, it should be noted that unless otherwise clearly defined and limited, term
" install ", " being connected ", " connection " should be interpreted broadly, and connect, also for example, it may be fixing
Can be to removably connect, or be integrally connected；Can be mechanically connected, it is also possible to be electrical connection or can
With mutual communication；Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, can be two
The connection of element internal or the interaction relationship of two elements.For those of ordinary skill in the art
Speech, can understand above-mentioned term concrete meaning in the present invention as the case may be.
In the present invention, unless otherwise clearly defined and limited, in second feature, it " goes up " fisrt feature
Or it " under " can include that the first and second features directly contact, it is also possible to include the first and second features
It not directly contact but by the other characterisation contact between them.And, fisrt feature is second
Feature " on ", " top " and " above " include fisrt feature directly over second feature and oblique on
Side, or it is merely representative of fisrt feature level height higher than second feature.Fisrt feature is at second feature " it
Under ", " lower section " and " below " include fisrt feature directly over second feature and oblique upper, or only
Only represent that fisrt feature level height is less than second feature.
Following disclosure provides many different embodiments or example for realizing the difference of the present invention
Structure.In order to simplify disclosure of the invention, hereinafter parts and setting to specific examples are described.
Certainly, they are the most merely illustrative, and are not intended to limit the present invention.Additionally, the present invention can be
Repeat reference numerals and/or reference letter in different examples, this repetition is to simplify and clearly mesh
, itself do not indicate the relation between discussed various embodiment and/or setting.Additionally, this
The bright various specific technique provided and the example of material, but those of ordinary skill in the art can anticipate
Know the application to other techniques and/or the use of other materials.
Please join Fig. 1～Fig. 3, the orifice plate 100 for centrifugally operated of the present invention the first better embodiment
Including base 102, multiple placement bar 104 and lid 106.Orifice plate 100 can use plastic cement to make.
This base 102 is in rectangular-shaped, and it offers multiple groove 108.In present embodiment, recessed
The quantity of groove 108 is 12, and 12 grooves 108 are each parallel to the minor face of base 102.Accordingly,
The quantity placing bar 104 is also 12.It is appreciated that in other embodiments, groove 108
Can be other quantity with the quantity of placement bar 104, depending on actually required.
Each placement bar 104 can releasably be arranged in the groove 108 of correspondence, this placement bar 104
Offer the multiple putting holes 110 for placing centrifuge tube (not shown).Centrifuge tube can be used for carrying out
The reaction experiment of PCR class.Owing to 12 grooves 108 are each parallel to the minor face of base 102, therefore,
Place the bar 104 minor face also parallel with base 102 for 12.
Specifically, the quantity of the putting hole 110 that each placement bar 104 is offered is 8, therefore,
The orifice plate 100 of present embodiment is 96 orifice plates, such as, can be used for the Nextera of 96 DNA sample
XT library construction (follow-up explanation).
It addition, for convenience of placing picking and placeing of bar 104, the opposing both sides of each placement bar 104 are provided with
Bearing and leaning portion 112, this bearing and leaning portion 112 is against the surface of this base 102.
This lid 106 can releasably cover and be located on this base 102 and the plurality of placement bar 104.Lid
Son 106 falls dust and other impurities in can preventing putting hole 110, keep the cleaning in putting hole 110.
In sum, in above-mentioned orifice plate 100, when being centrifuged operation, multiple centrifuge tubes can be placed
Place on bar 104 at one, the placement bar 104 being placed with multiple centrifuge tube is taken from base 102
It is placed on after going out on centrifuge head, therefore, once multiple centrifuge tubes can be centrifuged operation, it is to avoid
Uninteresting and the substantial amounts of duplication of labour, and then improve efficiency of research and development.
Please join Fig. 4～Fig. 5, the centrifuge 200 of the present invention the second better embodiment includes centrifuge machine
Body 202 and centrifuge head 204.
This centrifuge fuselage 202 is substantially in truncated cone-shaped.This centrifuge head 204 is arranged on this centrifuge
On fuselage 202.Centrifuge fuselage 202 can control centrifuge head 204 to being positioned over centrifuge machine
Centrifuge tube (not shown) on 204 is centrifuged operation.
This centrifuge head 204 includes support bar 206 and supporting part 208, and this support bar 206 is fixed
Bottom surface at this supporting part 208.
Specifically, this supporting part 208 includes support arm 210 and base plate 212.This support bar 206 is solid
It is scheduled on the bottom surface of this base plate 212.
The quantity of support arm 210 is two, and two support arms 210 are arranged on opposing the two of base plate 212
Side.It is preferred that these two support arms 210 are symmetrically disposed on this base plate 212, so, from
During heart operation, centrifuge head 204 is more stable when operating.
Each support arm 210 offers bar shape trench hole 214, and this bar shape trench hole 214 can be used for orifice plate 100
Placement bar 104.When placing bar 104 and being placed in bar shape trench hole 214, bearing and leaning portion 112 can be resisted against
On the surface of support arm 210, prevent from placing bar 104 falling during centrifugally operated.
When carrying out sample-adding operation, placement bar 104 is placed on base, as in figure 2 it is shown, will be centrifugal
Pipe (such as PCR pipe, not shown) is placed on placement bar 104 and carries out sample-adding operation.After sample-adding completes,
It is placed on centrifuge head 204 by placing bar 104 can be centrifuged together with centrifuge tube above,
As shown in Figure 4.
Centrifuge 200 and the use of orifice plate 100 of the present invention are described with concrete experiment below.
Experiment: the Nextera XT library construction of 96 DNA sample
1. 96 PCR pipe (centrifuge tube) are put in the orifice plate 100 being easy to be centrifuged.
2. in 96 PCR pipe, it is separately added into 10ul 2 × Tagment DNA Buffer (TD), 5ul
Amplicon Tagment Mix (ATM), 5ul 0.2ng/ul DNA sample, blows gently with rifle head
Make a call to 20 times, fully mix.
3. two row being easy on the orifice plate 100 that is centrifuged are placed bars 104 to take out, be placed on the centrifuge of correspondence
On head 204, take off from centrifuge head 204 after centrifugal 30s and put back in orifice plate；
4. repeat third step and just can complete the centrifugal of all PCR pipe for 5 times.
5. PCR pipe is placed in PCR instrument, reacts as follows: 55 DEG C, 5min；4 DEG C of Hold (heat lids
60℃)。
6., after reaction terminates, 96 PCR pipe are put back on the orifice plate 100 being easy to be centrifuged, is separately added into 5ul
Neutralize Tagment Buffer (NT), blows and beats gently 20 times with rifle head, fully mixes.
7. two row being easy on the orifice plate 100 that is centrifuged are placed bars 104 to take out, be placed on the centrifuge of correspondence
On head 204, take off from centrifuge head 204 after centrifugal 30s and put back in orifice plate；It is repeated 5 times the most permissible
Complete the centrifugal of all PCR pipe.
8. incubated at room 5min.
9. in 96 PCR pipe, it is separately added into 15ul Nextera PCR Master Mix (NPM), 5ul
Index 1Primers (N7XX), 5ul Index 2Primers (N5XX), blow gently with rifle head
Make a call to 20 times, fully mix.
10. two row being easy on the orifice plate 100 that is centrifuged are placed bars 104 to take out, be placed on the centrifugal of correspondence
On machine head 204, take off from centrifuge head 204 after centrifugal 30s and put back in orifice plate 100；Just it is repeated 5 times
The centrifugal of all PCR pipe can be completed.
PCR pipe is placed in PCR instrument by 11., reacts as follows:
12. magnetic beads for purifying, after this step, library construction completes；After quality inspection is qualified, be available on the machine order-checking.
In sum, in above-mentioned centrifuge 200, when being centrifuged operation, multiple centrifuge tubes can be put
Put and place on bar 104 at one, the placement bar being placed with multiple centrifuge tube is taken out from base 102
After be placed on the bar shape trench hole 214 of centrifuge head 204, therefore, can be once by multiple centrifuge tubes
It is centrifuged operation, it is to avoid the uninteresting and substantial amounts of duplication of labour, and then improves efficiency of research and development.
In the description of this specification, reference term " embodiment ", " some embodiments ",
The description of " exemplary embodiment ", " example ", " concrete example " or " some examples " etc.
Mean that the specific features, structure, material or the feature that combine this embodiment or example description are contained in this
In at least one embodiment of invention or example.In this manual, the schematic table to above-mentioned term
State and be not necessarily referring to identical embodiment or example.And, the specific features of description, structure, material
Material or feature can combine in any one or more embodiments or example in an appropriate manner.
While embodiments of the present invention have been illustrated and described, those of ordinary skill in the art is permissible
Understand: can these embodiments be carried out multiple in the case of without departing from the principle of the present invention and objective
Changing, revise, replace and modification, the scope of the present invention is limited by claim and equivalent thereof.
Claims (9)
1. the orifice plate for centrifugally operated, it is characterised in that include base and multiple placement bar,
This base offers multiple groove, and each placement bar can releasably be arranged in the groove of correspondence, and this is put
Put bar and offer the multiple putting holes for placing centrifuge tube.
2. orifice plate as claimed in claim 1, it is characterised in that the opposing both sides of each placement bar set
Being equipped with bearing and leaning portion, this bearing and leaning portion is against the surface of this base.
3. orifice plate as claimed in claim 1, it is characterised in that this orifice plate also includes lid, this lid
Son can releasably cover and be located on this base and the plurality of placement bar.
4. a centrifuge head, it is characterised in that include support bar and supporting part, this support bar is solid
Being scheduled on the bottom surface of this supporting part, this supporting part includes support arm, and this support arm offers bar shape trench hole, should
Bar shape trench hole is for placing the placement bar of orifice plate.
5. centrifuge head as claimed in claim 4, it is characterised in that this supporting part includes base plate,
The opposing both sides of this base plate are provided with two these support arms, and this support bar is fixed on the bottom surface of this base plate.
6. centrifuge head as claimed in claim 5, it is characterised in that these two support arm symmetries
Ground is arranged on which floor plate.
7. a centrifuge, it is characterised in that include centrifuge fuselage and centrifuge head, this is centrifuged
Machine head is arranged on this centrifuge machine, and this centrifuge head includes support bar and supporting part, this support
Bar is fixed on the bottom surface of this supporting part, and this supporting part includes support arm, and this support arm offers bar shape trench hole,
This bar shape trench hole is for placing the placement bar of orifice plate.
8. centrifuge as claimed in claim 7, it is characterised in that this supporting part includes base plate, should
The opposing both sides of base plate are provided with two these support arms, and this support bar is fixed on the bottom surface of this base plate.
9. centrifuge as claimed in claim 8, it is characterised in that these two support arms set symmetrically
Put on which floor plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510229249.4A CN106179782A (en) | 2015-05-07 | 2015-05-07 | For the orifice plate of centrifugally operated and centrifuge head and centrifuge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510229249.4A CN106179782A (en) | 2015-05-07 | 2015-05-07 | For the orifice plate of centrifugally operated and centrifuge head and centrifuge |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106179782A true CN106179782A (en) | 2016-12-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510229249.4A Pending CN106179782A (en) | 2015-05-07 | 2015-05-07 | For the orifice plate of centrifugally operated and centrifuge head and centrifuge |
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| CN (1) | CN106179782A (en) |
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| US4968625A (en) * | 1988-02-01 | 1990-11-06 | Difco Laboratories | Centrifrugation vial and cluster tray |
| GB2288233B (en) * | 1994-04-06 | 1998-10-28 | Akzo Nobel Nv | Microtitration plate |
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| US20020155035A1 (en) * | 2001-02-20 | 2002-10-24 | Manfred Kansy | Cuvette arrays |
| US20130029343A1 (en) * | 2010-02-22 | 2013-01-31 | 4Titude Ltd. | Multiwell strips |
| GB2494860A (en) * | 2011-09-07 | 2013-03-27 | Abgene Ltd | An array of PCR wells and an array of caps for such a well array |
| CN103084281A (en) * | 2012-09-14 | 2013-05-08 | 盛司潼 | Plate centrifuge |
| WO2014143044A1 (en) * | 2013-03-15 | 2014-09-18 | Becton, Dickinson And Company | Process tube and carrier tray |
| EP2810753A1 (en) * | 2013-06-06 | 2014-12-10 | 4titude Ltd. | Tear-away multi well plate |
| US20140361022A1 (en) * | 2013-06-11 | 2014-12-11 | J.G. Finneran Associates, Inc. | Rotation-limiting well plate assembly |
| CN204182489U (en) * | 2014-11-10 | 2015-03-04 | 杭州奥盛仪器有限公司 | Modified Microplate centrifuge |
| CN204194150U (en) * | 2014-10-24 | 2015-03-11 | 福州艾迪康医学检验所有限公司 | A kind of centrifuge |
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2015
- 2015-05-07 CN CN201510229249.4A patent/CN106179782A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3907505A (en) * | 1973-05-30 | 1975-09-23 | Miles Lab | Selectively detachable apparatus |
| US4968625A (en) * | 1988-02-01 | 1990-11-06 | Difco Laboratories | Centrifrugation vial and cluster tray |
| GB2288233B (en) * | 1994-04-06 | 1998-10-28 | Akzo Nobel Nv | Microtitration plate |
| EP1053790A2 (en) * | 1999-05-20 | 2000-11-22 | Advanced Biotechnologies Limited | Improved multi-well plates. |
| US20020155035A1 (en) * | 2001-02-20 | 2002-10-24 | Manfred Kansy | Cuvette arrays |
| US20130029343A1 (en) * | 2010-02-22 | 2013-01-31 | 4Titude Ltd. | Multiwell strips |
| GB2494860A (en) * | 2011-09-07 | 2013-03-27 | Abgene Ltd | An array of PCR wells and an array of caps for such a well array |
| CN103084281A (en) * | 2012-09-14 | 2013-05-08 | 盛司潼 | Plate centrifuge |
| WO2014143044A1 (en) * | 2013-03-15 | 2014-09-18 | Becton, Dickinson And Company | Process tube and carrier tray |
| EP2810753A1 (en) * | 2013-06-06 | 2014-12-10 | 4titude Ltd. | Tear-away multi well plate |
| US20140361022A1 (en) * | 2013-06-11 | 2014-12-11 | J.G. Finneran Associates, Inc. | Rotation-limiting well plate assembly |
| CN204194150U (en) * | 2014-10-24 | 2015-03-11 | 福州艾迪康医学检验所有限公司 | A kind of centrifuge |
| CN204182489U (en) * | 2014-11-10 | 2015-03-04 | 杭州奥盛仪器有限公司 | Modified Microplate centrifuge |
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Application publication date: 20161207 |
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