CN106178381A - A kind of oil stain remover based on microorganism - Google Patents
A kind of oil stain remover based on microorganism Download PDFInfo
- Publication number
- CN106178381A CN106178381A CN201610512395.2A CN201610512395A CN106178381A CN 106178381 A CN106178381 A CN 106178381A CN 201610512395 A CN201610512395 A CN 201610512395A CN 106178381 A CN106178381 A CN 106178381A
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- CN
- China
- Prior art keywords
- parts
- microorganism
- bacillus
- stain remover
- oil stain
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
Abstract
The invention discloses a kind of oil stain remover based on microorganism, solve existing microorganism the highest to the efficiency of the degraded of oil, there is degradation effect is not very good problem.The present invention includes that solid formulation and microbial inoculum, described solid formulation are made up of the composition of following weight portion: 2 (3,4 dihydroxy phenyl) ethamine 1～2 parts, polyglycolic acid resin 10～15 parts, lignin 1～2 parts, carbon tetrachloride 2～4 parts；Described microbial inoculum is made up of secondary Brevibacillus brevis, Bacillus and Saccharomycopsis lipolytica, and described microbial inoculum addition is 1 × 106～1 × 109cuf/g.The present invention has advantages such as being effectively improved oil degradation efficiency.
Description
Technical field
The present invention relates to a kind of stain remover, be specifically related to a kind of oil stain remover based on microorganism.
Background technology
Gasoline or petrol contaminated soil and subsoil water are application routine techniquess, such as soil steam extraction (SVE), utilize
Air injection (AS) enhancing carrys out scrubbing.Gasoline or petrol (such as hydraulic oil, motor oil and fuel oil) is high in underground environment
Spend degradable, thus, if needing to carry out considerable fraction of scrubbing, need to make great efforts the condition that holding is suitable, as in release
There is sufficient concentrations of oxygen in district, can accelerate the aerobic degradation of gasoline or petrol compound.
Routine techniques (such as SVE) by soil consistently with fresh air supplement and provide needed for oxygen supply, or
Air pulse is transported to underground by air injection by person's applied compression machine.Some scrubbing expert is even with chemicals (such as
Hydrogen peroxide eases up oxygen-releasing compound) aoxidize the gasoline of spilling and fuel oil and accelerate cleaning.
Existing a lot of employing microorganism degraded to oil in the industry, but the efficiency of degraded is the highest, there is degradation effect
It it not very good problem.
Summary of the invention
The technical problem to be solved is: existing microorganism is the highest to the efficiency of the degraded of oil, exists
Degradation effect is not very good problem, it is provided that solve a kind of based on microorganism the oil stain remover of the problems referred to above.
The present invention is achieved through the following technical solutions:
A kind of oil stain remover based on microorganism, including solid formulation and microbial inoculum, described solid formulation is by the composition of following weight portion
Composition: 2-(3,4-dihydroxy phenyl) ethamine 1～2 parts, polyglycolic acid resin 10～15 parts, lignin 1～2 parts, carbon tetrachloride
2～4 parts；Described microbial inoculum is made up of secondary Brevibacillus brevis, Bacillus and Saccharomycopsis lipolytica, described microbial inoculum addition
It is 1 × 106～1 × 109cuf/g。
By the cooperation of different strain, and cooperating by microbial inoculum and solid formulation, effectively make oil degradation efficiency have
Effect improves, and can effectively reduce the time of degraded, and effect is more significantly.
Further, described secondary Brevibacillus brevis is the Brevibacillus brevis of preserving number CGMCC NO. 1.6404, described
Bacillus is the Bacillus of preserving number CICC 23870, and described fat laminating adhesive spore yeast is preserving number CICC's 1460
Saccharomycopsis lipolytica.
Further, described secondary Brevibacillus brevis, viable bacteria ratio between Bacillus and Saccharomycopsis lipolytica
Example is 1～2: 4～5: 5～6.
In order to reach preferably effect, described solid formulation is made up of the composition of following weight portion: 2-(3,4-dihydroxy benzenes
Base) ethamine 1～2 parts, polyglycolic acid resin 13～14 parts, lignin 1～2 parts, carbon tetrachloride 2～3 parts.
As optimally proportioning, described solid formulation is made up of the composition of following weight portion: 2-(3,4-dihydroxy phenyl) second
Amine 2 parts, polyglycolic acid resin 13 parts, lignin 1 part, carbon tetrachloride 2 parts.
The present invention compared with prior art, has such advantages as and beneficial effect:
The present invention, by each constituent and the optimization of proportioning, effectively makes oil degradation efficiency be effectively improved, and can effectively subtract
The time of few degraded, effect is more significantly.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with embodiment, the present invention is made
Further describing in detail, the exemplary embodiment of the present invention and explanation thereof are only used for explaining the present invention, are not intended as this
The restriction of invention.
Embodiment
A kind of oil stain remover based on microorganism, including solid formulation and microbial inoculum, described solid formulation is by following weight portion
Composition forms: 2-(3,4-dihydroxy phenyl) ethamine 1～2 parts, polyglycolic acid resin 10～15 parts, lignin 1～2 parts, tetrachloro
Change carbon 2～4 parts；Described microbial inoculum is made up of secondary Brevibacillus brevis, Bacillus and Saccharomycopsis lipolytica, and described microbial inoculum adds
Dosage is 1 × 106～1 × 109cuf/g。
Described secondary Brevibacillus brevis is the Brevibacillus brevis of preserving number CGMCC NO. 1.6404, described bacillus
Belonging to the Bacillus for preserving number CICC 23870, described fat laminating adhesive spore yeast is the solution fat laminating adhesive of preserving number CICC 1460
Spore yeast.
In the present embodiment, the concrete parts by weight of each constituent are as shown in table 1.
Table 1
In above-mentioned table 1, A is Brevibacillus brevis, and B is Bacillus, and C is Saccharomycopsis lipolytica, and in the present embodiment, this removes
The concrete preparation method of dirty agent is as follows:
Step one, configuration solid formulation, the most sequentially add 2-(3,4-dihydroxy phenyl) ethamine, lignin and polyglycolic acid resin
Mix homogeneously in carbon tetrachloride；
Step 2, configuration microbial inoculum, this microbial inoculum is 1～the secondary Brevibacillus brevis of 2: 4～5: 5～6, brood cell's bar by number of viable ratio
Pseudomonas forms with Saccharomycopsis lipolytica；
Step 3 to, microbial inoculum is added mix homogeneously in solid formulation, after lyophilizing, i.e. make finished product.
In above-mentioned preparation method, step of freeze drying is routine techniques means, thus repeats no more.
The stain remover forming the material of above-mentioned weight portion is put in the oil rejected water that oil content is 98mg/L, the most right
Petroleum degradation rate during reaction 5h, 12h, 48h and 5d detects, and this testing result is as shown in table 2.
Table 2
By the test data of above-mentioned table 2, the data in present example 1-example 3 show: proportioning of the present invention, effectively increase
Add degradation rate, improve degradation efficiency.
Above-described detailed description of the invention, has been carried out the purpose of the present invention, technical scheme and beneficial effect further
Describe in detail, be it should be understood that the detailed description of the invention that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, all within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. done, all should comprise
Within protection scope of the present invention.
Claims (5)
1. an oil stain remover based on microorganism, it is characterised in that including solid formulation and microbial inoculum, described solid formulation is by following
Weight portion composition composition: 2-(3,4-dihydroxy phenyl) ethamine 1～2 parts, polyglycolic acid resin 10～15 parts, lignin 1～
2 parts, carbon tetrachloride 2～4 parts；Described microbial inoculum is made up of secondary Brevibacillus brevis, Bacillus and Saccharomycopsis lipolytica, institute
Stating microbial inoculum addition is 1 × 106～1 × 109cuf/g。
A kind of oil stain remover based on microorganism the most according to claim 1, it is characterised in that the short spore of described pair
Bacillus is the Brevibacillus brevis of preserving number CGMCC NO. 1.6404, and described Bacillus is preserving number CICC's 23870
Bacillus, described fat laminating adhesive spore yeast is the Saccharomycopsis lipolytica of preserving number CICC 1460.
A kind of oil stain remover based on microorganism the most according to claim 2, it is characterised in that the short spore of described pair
Viable bacteria ratio between bacillus, Bacillus and Saccharomycopsis lipolytica is 1～2: 4～5: 5～6.
A kind of oil stain remover based on microorganism the most according to claim 1, it is characterised in that described solid formulation by with
The composition composition of lower weight portion: 2-(3,4-dihydroxy phenyl) ethamine 1～2 parts, polyglycolic acid resin 13～14 parts, lignin 1
～2 parts, carbon tetrachloride 2～3 parts.
A kind of oil stain remover based on microorganism the most according to claim 1, it is characterised in that described solid formulation by with
The composition composition of lower weight portion: 2 parts of 2-(3,4-dihydroxy phenyl) ethamine, polyglycolic acid resin 13 parts, lignin 1 part, tetrachloro
Change 2 parts of carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610512395.2A CN106178381A (en) | 2016-07-04 | 2016-07-04 | A kind of oil stain remover based on microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610512395.2A CN106178381A (en) | 2016-07-04 | 2016-07-04 | A kind of oil stain remover based on microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106178381A true CN106178381A (en) | 2016-12-07 |
Family
ID=57463203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610512395.2A Withdrawn CN106178381A (en) | 2016-07-04 | 2016-07-04 | A kind of oil stain remover based on microorganism |
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| CN (1) | CN106178381A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117568239A (en) * | 2024-01-05 | 2024-02-20 | 成都医学院 | Brevibacillus paraBrevibacillus and application thereof in degradation and decoloration of aniline blue dye |
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| JP2006335746A (en) * | 2005-06-06 | 2006-12-14 | Bio Iryo Joho:Kk | Utilization of human physiologically active substance of dormant state edible microorganism |
| RU2337069C1 (en) * | 2007-04-02 | 2008-10-27 | Закрытое акционерное общество "Биоойл" | Preparation for soil and water purufication from oil and oil products |
| JP2011057968A (en) * | 2009-08-12 | 2011-03-24 | Fuji Mentenir Kk | Agent for treating oils, method for producing such treatment agent, and method for treating oils using the treatment agent |
| CN102459568A (en) * | 2009-05-26 | 2012-05-16 | 先进生物营养公司 | Stable dry powder composition comprising biologically active microorganisms and/or bioactive materials and methods of making |
| US20120156759A1 (en) * | 2009-06-15 | 2012-06-21 | Total Raffinage & Marketing | Method and product for decomposing organic compounds |
| CN102757920A (en) * | 2012-07-27 | 2012-10-31 | 长安大学 | Bacillus pumilus and application thereof |
| CN103058474A (en) * | 2012-07-10 | 2013-04-24 | 郑涵 | Bioremediation type oil-contained silt separating agent and preparation method thereof |
| CN103157392A (en) * | 2013-03-22 | 2013-06-19 | 清华大学 | Biodegradable oil-water separation net film with super-hydrophobic and super-oleophylic properties and preparation method thereof. |
| CN103394295A (en) * | 2013-08-14 | 2013-11-20 | 哈尔滨工业大学 | Hydrophilic PVDF (Polyvinylidene Fluoride) composite ultrafiltration membrane and preparation method thereof |
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2016
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Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006335746A (en) * | 2005-06-06 | 2006-12-14 | Bio Iryo Joho:Kk | Utilization of human physiologically active substance of dormant state edible microorganism |
| RU2337069C1 (en) * | 2007-04-02 | 2008-10-27 | Закрытое акционерное общество "Биоойл" | Preparation for soil and water purufication from oil and oil products |
| CN102459568A (en) * | 2009-05-26 | 2012-05-16 | 先进生物营养公司 | Stable dry powder composition comprising biologically active microorganisms and/or bioactive materials and methods of making |
| US20120156759A1 (en) * | 2009-06-15 | 2012-06-21 | Total Raffinage & Marketing | Method and product for decomposing organic compounds |
| JP2011057968A (en) * | 2009-08-12 | 2011-03-24 | Fuji Mentenir Kk | Agent for treating oils, method for producing such treatment agent, and method for treating oils using the treatment agent |
| CN103058474A (en) * | 2012-07-10 | 2013-04-24 | 郑涵 | Bioremediation type oil-contained silt separating agent and preparation method thereof |
| CN102757920A (en) * | 2012-07-27 | 2012-10-31 | 长安大学 | Bacillus pumilus and application thereof |
| CN103157392A (en) * | 2013-03-22 | 2013-06-19 | 清华大学 | Biodegradable oil-water separation net film with super-hydrophobic and super-oleophylic properties and preparation method thereof. |
| CN103394295A (en) * | 2013-08-14 | 2013-11-20 | 哈尔滨工业大学 | Hydrophilic PVDF (Polyvinylidene Fluoride) composite ultrafiltration membrane and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117568239A (en) * | 2024-01-05 | 2024-02-20 | 成都医学院 | Brevibacillus paraBrevibacillus and application thereof in degradation and decoloration of aniline blue dye |
| CN117568239B (en) * | 2024-01-05 | 2024-03-26 | 成都医学院 | Brevibacillus paraBrevibacillus and application thereof in degradation and decoloration of aniline blue dye |
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Application publication date: 20161207 |