CN106177944A - A kind of adjuvant for chicken Newcastle disease live vaccine - Google Patents
A kind of adjuvant for chicken Newcastle disease live vaccine Download PDFInfo
- Publication number
- CN106177944A CN106177944A CN201610684312.8A CN201610684312A CN106177944A CN 106177944 A CN106177944 A CN 106177944A CN 201610684312 A CN201610684312 A CN 201610684312A CN 106177944 A CN106177944 A CN 106177944A
- Authority
- CN
- China
- Prior art keywords
- adjuvant
- newcastle disease
- live vaccine
- alumen
- disease live
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5254—Virus avirulent or attenuated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55505—Inorganic adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18111—Avulavirus, e.g. Newcastle disease virus
- C12N2760/18134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention discloses a kind of adjuvant for chicken Newcastle disease live vaccine, Alumen, vitamin E, levamisole, lanoline are organically combined by the present invention, coordinate mutually, prepare the immunity composite adjuvant for chicken Newcastle disease live vaccine, play the sharpest edges of adjuvant, share immune effect preferably with antigen, toxicity is little, low cost.
Description
Technical field
The present invention relates to vaccine adjuvant technical field, particularly relate to a kind of adjuvant for chicken Newcastle disease live vaccine.
Background technology
Adjuvant is nonspecific immunity strengthening agent, when injecting together with antigen or being previously implanted body, and can enhancing body
Immunne response or change type of immune response to antigen.It is the physics by changing antigen that adjuvant strengthens the mechanism of immunne response
Shape, extends antigen retention time in body；Stimulate the mononuclear phagocyte submission ability to antigen；Lymphocyte is stimulated to divide
Change, increase and expand immunne response ability.
Adjuvant has a variety of；Such as aluminum hydroxide adjuvant, coryne bacterium parvum, lipopolysaccharide, cytokine, Alumen etc..In vain
Oil adjuvanted immunogenic is good, but toxic and side effects is big；Aluminium hydroxide gel adjuvanted immunogenic is good, and side effect is little, but can not induce
Cellular immunization；Lipoid adjuvant and propolis adjuvant immunogenicity are good, can induce humoral immunization and cellular immunization, immunity persistent period
Long, but the prices of raw materials are expensive.Existing immunological adjuvant, because weak effect or virulence are big, especially lacking of property, do not have
One obtains promising result.Therefore it is an object of the invention to provide a kind of Novel immune for chicken Newcastle disease live vaccine to help
Agent.
Summary of the invention
The purpose of the present invention is aiming at the defect of above-mentioned existence and provides a kind of adjuvant for chicken Newcastle disease live vaccine,
Alumen, vitamin E, levamisole, lanoline are organically combined by the present invention, coordinate mutually, prepare for newcastle disease epidemic disease alive
The immunity composite adjuvant of Seedling, plays the sharpest edges of adjuvant, share immune effect preferably with antigen, and toxicity is little, low cost.
A kind of adjuvant technical scheme for chicken Newcastle disease live vaccine of the present invention is, including Alumen, vitamin E, left-handed
Imidazoles, lanoline.
Described a kind of adjuvant for chicken Newcastle disease live vaccine, the following component including by weight: Alumen 20-60,
Vitamin e1 0-20, levamisole 1-8, lanoline 10-30.
Described a kind of adjuvant for chicken Newcastle disease live vaccine, the following component including by weight: Alumen 30-50,
Vitamin e1 2-18, levamisole 2-6, lanoline 12-25.
Described a kind of adjuvant for chicken Newcastle disease live vaccine, the following component including by weight: Alumen 40-45,
Vitamin e1 2-16, levamisole 3-5, lanoline 15-20.
Described a kind of adjuvant for chicken Newcastle disease live vaccine, it is preferred that include following component by weight: Alumen
45, vitamin e1 5, levamisole 5, lanoline 16.
Described a kind of adjuvant for chicken Newcastle disease live vaccine, preparation method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 10-20%；
(2) vitamin E, levamisole, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
In step (1), Alumen is configured to the aluminum potassium sulfate solution of 12%.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 2500rpm, emulsifying 30 minutes.
The invention have the benefit that a kind of adjuvant for chicken Newcastle disease live vaccine of the present invention, including Alumen, dimension
Raw element E, levamisole, lanoline.Alumen, vitamin E, levamisole, lanoline are organically combined by the present invention, coordinate mutually,
Prepare the immunity composite adjuvant for chicken Newcastle disease live vaccine, play the sharpest edges of adjuvant, share immune effect with antigen
Preferably, toxicity is little, low cost.
Adjuvant of the present invention is combined with chicken Newcastle disease live vaccine antigen, forms optimal formula, i.e. forms immunostimulation and is combined
Thing, preferably plays immunological adjuvant effect.Apply this adjuvant and be used alone adsorbed onto alum adjuvant or use existing Freund adjuvant,
It is respectively configured chicken Newcastle disease live vaccine live vaccine immune animal, applies its antibody titer of adjuvant group of the present invention obviously higher than individually
Use adsorbed onto alum adjuvant or use existing Freund adjuvant experimental group, exceeding 5-12 antibody titer.
Detailed description of the invention:
In order to be more fully understood that the present invention, describe technical scheme in detail with instantiation below, but the present invention
It is not limited thereto.
Embodiment 1
Described a kind of adjuvant for chicken Newcastle disease live vaccine, the following component including by weight: Alumen 45, vitamin
E15, levamisole 5, lanoline 16.
Preparation method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 12%；
(2) vitamin E, levamisole, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 2500rpm, emulsifying 30 minutes, obtains vaccine.
According to national basic veterinary drug standard, verify its result:
Safety verification: with the yellow-feather broiler 15 at 1-2 monthly age, every intramuscular injection vaccine 0.5ml.Observe 24 hours, all should be without exception
Reaction.After observing 10, all there is not abnormal response in 15 chickens.
Efficacy test: immune group with the susceptible yellow-feather broiler of the health at 1 to 2 monthly age (by 92 editions regulations
Annex page 15 does suppression hemagglutination test (HA test), and suppression agglutination titer should be less than 1: 4) 10, every intramuscular injection vaccine 0.5ml, 10
After 14 days, together with the non-immunized controls chicken 3 that condition is identical, the Beijing Strain newcastle disease of each intramuscular injection 10 4ELD50 is strong
Poison 1ml, observes 10 to 14 days, and comparison chicken all falls ill death, and immune chicken at least protects 9.
Assay: comparison chicken falls ill entirely, immune group is entirely strong lives.
Embodiment 2
Described a kind of adjuvant for chicken Newcastle disease live vaccine, the following component including by weight: Alumen 40, vitamin
E12, levamisole 3, lanoline 20.
Preparation method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 10%；
(2) vitamin E, levamisole, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
Antigen is added in adjuvant according to the weight ratio of 1:1, according to 2500rpm, emulsifying 30 minutes, obtains vaccine.
According to national basic veterinary drug standard, verify its result:
Safety verification: with the yellow-feather broiler 15 at 1-2 monthly age, every intramuscular injection vaccine 0.5ml.Observe 24 hours, all should be without exception
Reaction.After observing 10, all there is not abnormal response in 15 Sanhuang chicken chickens.
Efficacy test: immune group with the susceptible yellow-feather broiler of the health at 1 to 2 monthly age (by 92 editions regulations
Annex page 15 does suppression hemagglutination test (HA test), and suppression agglutination titer should be less than 1: 4) 10, every intramuscular injection vaccine 0.5ml, 10
After 14 days, together with the non-immunized controls chicken 3 that condition is identical, the Beijing Strain newcastle disease of each intramuscular injection 10 4ELD50 is strong
Poison 1ml, observes 10 to 14 days, and comparison chicken all falls ill death, and immune chicken at least protects 9.
Assay: comparison chicken falls ill entirely, immune group is entirely strong lives.
Claims (8)
1. the adjuvant for chicken Newcastle disease live vaccine, it is characterised in that include Alumen, vitamin E, levamisole, Pilus Caprae seu Ovis
Fat.
A kind of adjuvant for chicken Newcastle disease live vaccine the most according to claim 1, it is characterised in that include by weight
Following component: Alumen 20-60, vitamin e1 0-20, levamisole 1-8, lanoline 10-30.
A kind of adjuvant for chicken Newcastle disease live vaccine the most according to claim 1, it is characterised in that include by weight
Following component: Alumen 30-50, vitamin e1 2-18, levamisole 2-6, lanoline 12-25.
A kind of adjuvant for chicken Newcastle disease live vaccine the most according to claim 1, it is characterised in that include by weight
Following component: Alumen 40-45, vitamin e1 2-16, levamisole 3-5, lanoline 15-20.
A kind of adjuvant for chicken Newcastle disease live vaccine the most according to claim 1, it is characterised in that include by weight
Following component: Alumen 45, vitamin e1 5, levamisole 5, lanoline 16.
A kind of adjuvant for chicken Newcastle disease live vaccine the most according to claim 1, it is characterised in that preparation method is:
(1) Alumen is configured to the aluminum potassium sulfate solution of 10-20%；
(2) vitamin E, levamisole, lanoline are added in aluminum potassium sulfate solution, mix homogeneously, adjust pH to be helped to 6.5
Agent.
A kind of adjuvant for chicken Newcastle disease live vaccine the most according to claim 6, it is characterised in that in step (1), will
Alumen is configured to the aluminum potassium sulfate solution of 12%.
A kind of adjuvant for chicken Newcastle disease live vaccine the most according to claim 6, it is characterised in that by antigen according to 1:
The weight ratio of 1 adds in adjuvant, according to 2500rpm, emulsifying 30 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610684312.8A CN106177944A (en) | 2016-08-18 | 2016-08-18 | A kind of adjuvant for chicken Newcastle disease live vaccine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610684312.8A CN106177944A (en) | 2016-08-18 | 2016-08-18 | A kind of adjuvant for chicken Newcastle disease live vaccine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106177944A true CN106177944A (en) | 2016-12-07 |
Family
ID=57522771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610684312.8A Pending CN106177944A (en) | 2016-08-18 | 2016-08-18 | A kind of adjuvant for chicken Newcastle disease live vaccine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106177944A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107837393A (en) * | 2017-10-27 | 2018-03-27 | 贵州福斯特生物科技有限公司 | Poultry live vaccine dilution adjuvant and preparation method and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1763100A (en) * | 2005-09-27 | 2006-04-26 | 东华大学 | Dipropyl phthalic acid antibody and its preparation method |
| CN1895266A (en) * | 2006-06-09 | 2007-01-17 | 甘肃省畜牧兽医研究所 | Compound slow-releasing immune enhancer for cock |
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| CN102647977A (en) * | 2009-10-08 | 2012-08-22 | 阿祖瑞博有限公司 | Formulation of drugs and vaccines in the form of percutaneous injectable needles |
| CN104940921A (en) * | 2015-07-07 | 2015-09-30 | 青岛易邦生物工程有限公司 | H9 subtype avian influenza virus inactivated vaccine including chicken a-interferon protein |
-
2016
- 2016-08-18 CN CN201610684312.8A patent/CN106177944A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1763100A (en) * | 2005-09-27 | 2006-04-26 | 东华大学 | Dipropyl phthalic acid antibody and its preparation method |
| CN1895266A (en) * | 2006-06-09 | 2007-01-17 | 甘肃省畜牧兽医研究所 | Compound slow-releasing immune enhancer for cock |
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| CN102647977A (en) * | 2009-10-08 | 2012-08-22 | 阿祖瑞博有限公司 | Formulation of drugs and vaccines in the form of percutaneous injectable needles |
| CN104940921A (en) * | 2015-07-07 | 2015-09-30 | 青岛易邦生物工程有限公司 | H9 subtype avian influenza virus inactivated vaccine including chicken a-interferon protein |
Non-Patent Citations (2)
| Title |
|---|
| 王卫红等: ""黏膜免疫佐剂的筛选II.ELISA检测"", 《湖北畜牧兽医》 * |
| 王永芬等: "《动物生物制品技术》", 31 August 2011 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107837393A (en) * | 2017-10-27 | 2018-03-27 | 贵州福斯特生物科技有限公司 | Poultry live vaccine dilution adjuvant and preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Watkins et al. | Safe recombinant outer membrane vesicles that display M2e elicit heterologous influenza protection | |
| Baldwin et al. | Enhanced humoral and Type 1 cellular immune responses with Fluzone® adjuvanted with a synthetic TLR4 agonist formulated in an emulsion | |
| Duthie et al. | Use of defined TLR ligands as adjuvants within human vaccines | |
| Zhao et al. | O/W nanoemulsion as an adjuvant for an inactivated H3N2 influenza vaccine: based on particle properties and mode of carrying | |
| Ahmad et al. | Alternative therapeutic strategies for histomonosis: a review. | |
| CN103083659A (en) | Preparation method and application of novel oil-free adjuvant | |
| Park et al. | Nanoformulated single‐stranded RNA‐based adjuvant with a coordinative amphiphile as an effective stabilizer: inducing humoral immune response by activation of antigen‐presenting cells | |
| KR101169196B1 (en) | Composition of adjuvant supplements and virus vaccine for fish | |
| CN102743750A (en) | Compound immunoenhancement agent, vaccine for birds and method for preparing compound immunoenhancement agent | |
| CN106177944A (en) | A kind of adjuvant for chicken Newcastle disease live vaccine | |
| KR102360690B1 (en) | Swine vaccine against prrs and lawsonia intracellularis | |
| Radmehri et al. | Comparative study on the efficacy of MF 59, ISA70 VG, and nano-aluminum hydroxide adjuvants, alone and with nano-selenium on humoral immunity induced by a bivalent newcastle+ avian influenza vaccine in chickens | |
| Sisteré-Oró et al. | Influenza NG-34 T cell conserved epitope adjuvanted with CAF01 as a possible influenza vaccine candidate | |
| KR100753675B1 (en) | The preparing method of inactivation vaccine of swine Erysipelas treated with specific antibody against antigen | |
| Awaad et al. | The effect of a combination of β (1-3) D-Glucan and Propionibacterium granulosum on productive performance and immune modulation of immunocompromised and non-immunocompromised broiler chickens. | |
| WO2019138210A1 (en) | Immunogenic agent and associated compositions, uses and methods | |
| Kumar et al. | Improved humoral immune response of oil adjuvant vaccine by saponin coadjuvantation against haemorrhagic septicaemia in mice and buffalo calves | |
| WO2016017620A1 (en) | Salmonella vaccine | |
| US20200289642A1 (en) | Compositions for Immunotherapy | |
| Suresh | Evaluating the protective efficacy of chitosan-nanoparticle based adjuvanted oral Salmonella subunit vaccine against bacterial colonization in broilers | |
| Amer et al. | Effect of Antibiotic and Prebiotic on Immunity and Reproductivity of Broiler Breeder Flock | |
| TW201800577A (en) | Vaccine for poultry VII genotype newcastle disease and its preparation method capable of effectively inhibiting the newcastle disease using a de-activation vaccine | |
| CN106177943A (en) | A kind of adjuvant for infectious bronchitis vaccines | |
| Alazzouny et al. | Effect of Mycotoxins or Hard Water on Newcastle, Gumboro, and Infectious Bronchitis Post Vaccinal Immune Responses and Performance of Broilers. | |
| WO2021101187A3 (en) | Novel vaccine immune adjuvant composition containing bavachin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161207 |