CN106177935A - A kind of ruminant clostridial disease tetrad inactivated vaccine and preparation method thereof - Google Patents
A kind of ruminant clostridial disease tetrad inactivated vaccine and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of ruminant clostridial disease tetrad inactivated vaccine and preparation method thereof, i.e. clostridium septicum, C. perfringens Type C, bacillus perfringens D type, clostridium chauvei tetrad inactivated vaccine.Use this vaccine can prevent four kinds of common clostridial diseases of cattle and sheep by primary immune response, for cattle, sheep rauschbrand (blackleg), malignant edema (clostridium septicum), the prevention of enterotoxemia (C, D type), this vaccine Main Ingredients and Appearance has clostridium chauvei and the inactivated bacterial liquid of clostridium septicum, c-type bacillus perfringens toxoid and D type bacillus perfringens toxoid.
Description
Technical field
The present invention relates to a kind of ruminant clostridial disease tetrad inactivated vaccine and preparation method thereof.It is i.e. that a kind of prevention is by corruption
Lose the ruminant clostridial diseases such as cattle, sheep that clostridium, C. perfringens Type C, bacillus perfringens D type and clostridium chauvei cause
Tetrad inactivated vaccine and preparation method thereof.Belong to veterinary biologics field.
Background technology
Clostridial disease is the general name of the disease caused by pathogenic strain in fusobacterium (Clostridium).Clostridium is anaerobism
Property antibacterial, have more than 60 to plant, common pathogenic bacterium only more than 10 is planted, and is present in soil, sewage and people and the excrement of various animal more
Just in.Gram’s staining is the positive, in addition to minority strain, and all amphitrichous, can move and form brood cell, its diameter is more than bacterium
Body.Most strains can produce violent extracellular toxin, and it is pathogenic Main Factors, is again major antigen, is transformed into toxoid
After can stimulating animal produce antitoxin, can be used for preventing corresponding clostridial disease.Clostridial disease morbidity is rapidly, the course of disease is short, mortality rate is high.
Domestic animal is more common in ruminant such as cattle, sheep etc..
Obligate anaerobe clostridium chauvei (Clostridium chauvoei) can cause cattle, sheep blackleg.Cattle from
So infect mostly through digestive tract, individually through body surface traumatic infection.Sheep is traumatic infection mostly, but rare.Incubation period
Generally 3～5 days.The normal sudden onset of ill domestic animal, spirit is depressed, stops ruminating, and body temperature raises, and occurs features described above symptom therewith.
The malignant edema that clostridium septicum (Clostridium septicum) causes is a kind of acute infectious disease, most scattered
Send out, ruminate in beast the most susceptible with sheep.Sometimes occur in popularity.Soliped also can infect, and carnivore and poultry are rare.
By the title malignant edema of trauma infection contamination；By the epidemic disease of expressing one's gratification of digestive tract infection, sheep is suffered from belongs to the latter more, and death is quickly.People also may be used
Infect, mostly be trauma infection contamination, commonly referred to as gas gangrene.The wound circumference generation diffusivity inflammatory edema of trauma infection contamination, during beginning
Solid, scorching hot, pain, then painless without heat, hand pressure is soft.Along with the development of inflammatory edema, ill domestic animal General Symptoms aggravates, and breathes
Difficulty, mucosa cyanosis, pulse is tiny, and body temperature rises, and sometimes suffers from diarrhoea, can be dead within half a day to 3 day.Through digestive tract infection
Sick sheep sudden onset, during walking, hind leg waves, and head and neck bends backward, grinds one's teeth in sleep, mouth and nose flow out foam, spasm falls down to the ground, the most extremely
Die.
In humans and animals intestinal, bacillus perfringens is one of normal flora, can cause the alimentary toxicosis of people, intestinal poison
Mass formed by blood stasis, necrotic enteritis are suffered from diarrhoea, and the intestinal that the bacillus perfringens of all kinds is all likely to result in adult dairy cattle and calf is comprehensive
Disease.This bacterioid also can infect the mankind and other animal simultaneously, including pig, chicken and sheep.Although bacillus perfringens has a lot
Different hypotypes (A～E hypotype), can secrete different toxin and cause different diseases, but the clinical condition that different subtype causes
Shape and virulence factor can overlap, and the most do not make clear and definite differentiation.Bacillus perfringens is widely present in nature
In the gastrointestinal tract of environment and major part mammal.C-type bacillus perfringens (Clostridium perfrinens type C)
Can cause struck.The cause of disease of this disease pathogen and enterotoxemia belongs to and belongs to antibacterial of the same race together, but bacterial type is different.Pathogen is through mouthfeel
Dye, produces toxin in digestive tract breeding.Sudden onset, the course of disease is very brief, is barely perceivable symptom and i.e. accuses death, is difficult to before death
Make a definite diagnosis.Bacillus perfringens C can cause new-born calve intestinal gangrene.Ill calf is likely to before showing symptom of diarrhea the most
Through death.D type bacillus perfringens (Clostridium perfrinens type D) can cause enterotoxemia.Main danger
Evil sheep, especially with less than 2 years old sheep in good condition for very.Goat is rare.Ill domestic animal is dead rapidly, after death cuts open inspection kidney many in soft
Change, therefore also known as pulpy kidney disease.By digestive tract infection, more susceptible disease when fiber content is less and protein concentrate is too much in feedstuff, thus
Also referred to as " binge eating ".Symptom is similar with struck.Bacillus perfringens D can cause typical surfeit, and lamb is easier to sense than calf
Contaminate this syndrome.Health and the good calf that searches for food sudden death would generally be caused.There is also other nervous symptoms, including: limb
Body is inharmonious, trembles, and lies down later or spasm.
Changing daily ration, wound, management adjusts, can create suitable clostridium when parasite or other abnormal situation
The environment of growth, clostridium can manufacture and secrete potent toxins.Generally clostridium infection prognosis is very poor, and when often finding, animal is the most
Dead.Due to treatment success rate the lowest, so this disease focus on prevention, large-scale immunoprophylaxis can effectively reduce
The loss that this disease causes, has primary immune response at present or not and relevant development of vaccine of above-mentioned four kinds of clostridial diseases just can be prevented to report
Road.
Summary of the invention
It is an object of the invention to provide a kind of clostridium septicum, C. perfringens Type C, bacillus perfringens D type, edema due to disorder of QI
Cellulitis clostridium tetrad inactivated vaccine, be used for preventing cattle, sheep rauschbrand (blackleg), malignant edema (clostridium septicum), enterotoxemia (C,
D type).This vaccine main component has clostridium chauvei and the inactivated bacterial liquid of clostridium septicum, c-type bacillus perfringens toxoid and D
Type bacillus perfringens toxoid.
Technical scheme
1. a ruminant clostridial disease tetrad inactivated vaccine, it is characterised in that this vaccine main component has clostridium chauvei
Inactivated bacterial liquid, c-type bacillus perfringens toxoid and D type bacillus perfringens toxoid with clostridium septicum.
2. a kind of ruminant clostridial disease tetrad inactivated vaccine as claimed in claim 1, it is characterised in that described tetrad
The seedling production bacterial strain of inactivated vaccine is clostridium septicum C55-1 strain, c-type bacillus perfringens C59-2 strain, D type perfringens
Clostridium C60-2 strain and clostridium chauvei C54-1 strain.
The preparation method of a kind of ruminant clostridial disease tetrad inactivated vaccine the most as claimed in claim 1 or 2, its feature
It is that described vaccine preparation method is:
(1) described clostridium chauvei and clostridium septicum production bacterial strain bacterium solution are inoculated appropriate media to cultivate, warp
Collect after crossing inactivation and concentrating and concentrate bacterium solution；C-type bacillus perfringens and D type bacillus perfringens production bacterial strain bacterium solution are divided
Jie Zhong not cultivate by appropriate media, the detoxification collecting preparation after inactivation, detoxification, concentration concentrates bacterium solution；
(2) ruminant clostridium is obtained after adding adjuvant mixing after four kinds of concentration bacterium solution made above being mixed in proportion
Sick tetrad inactivated vaccine.
The specific embodiment of the invention
One, prepared by vaccine
1. this vaccine main component of main component has clostridium chauvei and the inactivated bacterial liquid of clostridium septicum, c-type perfringens
Clostridium toxoid and D type bacillus perfringens toxoid.
2. strain the present invention relates to clostridium chauvei C54-2 strain, clostridium septicum C55-1 strain, c-type bacillus perfringens C59-
(refer to ask for an interview China Veterinery Drug Inspection Office, China's veterinary microorganism strain is protected for 2 strains and D type bacillus perfringens C60-2 strain
Hide administrative center to write, China veterinary's strain catalogue (second edition), Scientia Agricultura Sinica technology publishing house, 2002, p 40,
52,48,50) strain is purchased from veterinary microorganism culture presevation administrative center of China of China Veterinery Drug Inspection Office.
3. culture medium clostridium septicum trypsinization steamed beef soup, bacillus perfringens meat liver gastric enzyme digestion soup or fish liver meat
Gastric enzyme digestion soup, clostridium chauvei liver bouillon of being sick of, it is purchased from rainforest biological engineering company limited of Rongcheng City.
4. prepared by seedling bacterium solution
(1) seedling bacterium solution can carry out quiescent culture by vial or culture tank；
(2) culture process parameter (being shown in Table 1)
Table 1 culture process parameter
(3) toxin determination:
1) the centrifugal supernatant of clostridium septicum cultivation bacterium solution, intravenous injection body weight 16～20g white mice 0.005～0.01ml,
Should be dead in 48 hours.
2) the cultivation bacterium solution of C. perfringens Type C is centrifuged supernatant, intravenous injection body weight 16～20g white mice 0.001ml,
Should be dead in 24 hours.
3) the cultivation bacterium solution of bacillus perfringens D type bacterium solution after pancreatin activates is centrifuged supernatant, intravenous injection body weight 16
～20g white mice 0.00025～0.0001ml, 0.001ml, should be dead in 24 hours.
(4) inactivateing or inactivate detoxification and add formalins by four kinds of bacterium solution cultivation amounts, addition is bacterium solution cultivation amount
0.4%～0.8%, inactivation time is clostridium chauvei 72～96 hours, clostridium septicum 72～144 hours, bacillus perfringens
120～192 hours.Every day fully vibrates or stirs.
(5) after inactivation check sampling, the lonely liver bouillon of inoculation is tested, and answers asepsis growth.
(6) detoxification inspection is with each 2～5 of each bacterium solution centrifuged supernatant intravenous injection body weight 16～20g white mice, every
0.5ml, observes 24 hours, and mouse survival is qualified for inactivation detoxification.
5. concentrate and join Seedling
By gentle to inactivation inspection, the detoxification qualified clostridium septicum of inspection, C. perfringens Type C, bacillus perfringens D type
Swollen cellulitis clostridium bacterium solution uses centrifugal method separating thallus and toxoid bacterium solution, collects thalline and the toxoid of four kinds of bacterium solution respectively
Bacterium solution mixes after carrying out joining Seedling and adding adjuvant, obtains aqueous vaccine.
6. minimum immune dosage measures
Clostridium septicum, c-type bacillus perfringens, D type bacillus perfringens minimum immune dosage measure: by the corruption prepared
Clostridium, C. perfringens Type C, bacillus perfringens D type bacterium solution make single Seedling.It is divided into not with the rabbit of body weight 1.5～2.0kg
With dosage group, each dosage group 5, subcutaneously or intramuscularly inject various dose vaccine, after immune 14～21 days, identical together with condition
Comparison rabbit 5, the respectively various toxin of intravenous injection minimum lethal dose, comparison exempts from whole death, and each bacterial type vaccine minimum is exempted from
Epidemic disease dose immunization rabbit protects at least 4.
Clostridium chauvei minimum immune dosage measures: make single Seedling by the clostridium chauvei bacterium solution prepared.By body weight 350～
The Cavia porcellus of 450g is divided into various dose group, each dosage group 5, and intramuscular injection various dose vaccine, after immune 21 days, together with bar
The comparison Cavia porcellus 5 that part is identical, 24 hours clostridium chauvei bacterium solution at least 0.2ml that each intramuscular injection is cultivated, observe 10, right
According to Cavia porcellus all death in 72 hours, minimum immune dosage group should protect at least 4.
Two, the safety verification of vaccine and efficacy test
(1) safety verification (following methods one-out-three)
1) clostridium septicum, C. perfringens Type C and bacillus perfringens D type three component
With family test-free vaccination body weight 1.5～2.0kg rabbit 4～5, each intramuscular injection 2ml (5 part sheep immunity
Dosage), observe 7～10, work all should be good for；
Check vaccination 3 monthly age above calf 3～5, each intramuscular injection 2～5 part cattle immunizing doses with cattle, observe
7～10, all should have no adverse reaction；
By the sheep inspection healthy susceptible sheep 3 of vaccination～5, each intramuscular injection 2～5 part sheep immunizing doses, observe 7～
10, all should have no adverse reaction.
2) blackleg part
With body weight 350～450g Cavia porcellus 2, each subcutaneous injection vaccine 2ml (is equivalent to 2 sheep immunizing doses or cattle 1 is exempted from
Epidemic disease dosage), observe 7～10, work all should be good for.
(2) efficacy test
Clostridium septicum, c-type bacillus perfringens and D type bacillus perfringens part with the rabbit of body weight 1.5～3.0kg or
Healthy susceptible sheep, packet immunity, often group 5, subcutaneously or intramuscularly vaccinates, rabbit immunization dosage 0.5ml, sheep 1ml.Exempt from
Epidemic disease 14～after 21 days, the comparison rabbit identical together with condition or sheep 5, inject toxin and the bacterium solution of lethal dose respectively, comparison rabbit or
Sheep is the most dead, and each group immunity rabbit or sheep at least protect more than 4 for qualified.
Clostridium chauvei the part Cavia porcellus 5 of body weight 350～450g, every intramuscular injection 1ml, after immune 21 days, connect
The comparison Cavia porcellus identical with condition 5,24 hours clostridium chauvei bacterium solution at least 0.2ml that each intramuscular injection is cultivated, observe 10
Day, comparison Cavia porcellus is the most dead in 72 hours, and minimum immune dosage group should protect at least 4.
The microbial resources information that the present invention relates to
The present invention relates to clostridium chauvei C54-2 (CVCC No.60002), clostridium septicum C55-1 strain (CVCC
No.60021), c-type bacillus perfringens C59-2 strain (CVCC No.60102) and D type bacillus perfringens C60-2 strain (CVCC
No.60201) (refer to ask for an interview China Veterinery Drug Inspection Office, veterinary microorganism culture presevation administrative center of China writes, in
State veterinary strain catalogue (second edition), Scientia Agricultura Sinica technology publishing house, p 40,52,48,50 in 2002) during strain is purchased from
State's veterinary medicament supervision institute's bacterium kind preservation center.
Advantages of the present invention
The present invention relates to a kind of ruminant clostridial disease tetrad inactivated vaccine and preparation method thereof, i.e. clostridium septicum, aerogenesis
Capsular clostridium c-type, bacillus perfringens D type, clostridium chauvei tetrad inactivated vaccine.This vaccine is used to prevent by primary immune response
Four kinds of common clostridial diseases of cattle and sheep, for cattle, sheep rauschbrand (blackleg), malignant edema (clostridium septicum), enterotoxemia (C, D type)
Prevention, this vaccine main component has an inactivated bacterial liquid of clostridium chauvei and clostridium septicum, c-type bacillus perfringens toxoid and
D type bacillus perfringens toxoid.
Embodiment
Embodiments of the invention are for further describing technical scheme, but are not construed as limiting the invention.
Embodiment 1
The preparation of ruminant clostridial disease tetrad inactivated vaccine
1. strain clostridium chauvei C54-2 strain, clostridium septicum C55-1 strain, c-type bacillus perfringens C59-2 strain and D type produce
Gas capsular clostridium C60-2 strain (refers to ask for an interview China Veterinery Drug Inspection Office, veterinary microorganism culture presevation administrative center of China
Write, China veterinary's strain catalogue (second edition), Scientia Agricultura Sinica technology publishing house, 2002, p40,52,48,50) strain is equal
Purchased from China Veterinery Drug Inspection Office's bacterium kind preservation center, as production strain.
2. culture medium clostridium septicum trypsinization steamed beef soup, bacillus perfringens is with meat liver gastric enzyme digestion soup (or fish liver
Meat gastric enzyme digestion soup), clostridium chauvei liver bouillon of being sick of, it is purchased from rainforest biological engineering company limited of Rongcheng City.
3. prepared by seedling bacterium solution
(1) connect poison and take four kinds of clostridium production strains, clostridium chauvei C54-2 strain, clostridium septicum C55-1 strain, c-type respectively
Bacillus perfringens C59-2 strain and D type bacillus perfringens C60-2 strain, according to inoculum concentration 5% (V/V) equipped with the most suitable
The 500 of culture medium, inoculation in 000ml culture tank (culture medium loading amount is 400,000ml), cultivate according to table 2 parameter:
Table 2 culture process parameter
(2), after toxin determination has been cultivated, carry out cultivating the toxin determination of bacterium solution.Should reach following standard:
Clostridium septicum cultivates the centrifugal supernatant of bacterium solution, intravenous injection body weight 16～20g white mice 0.005～0.01ml, answers
Death in 48 hours.The cultivation bacterium solution of C. perfringens Type C is centrifuged supernatant, intravenous injection body weight 16～20g white mice
0.001ml, should be dead in 24 hours.The cultivation bacterium solution of bacillus perfringens D type bacterium solution after pancreatin activates is centrifuged supernatant,
Intravenous injection body weight 16～20g white mice 0.00025～0.0001ml, 0.001ml, should be dead in 24 hours.
(3) inactivation detoxification adds formalin by four kinds of bacterium solution cultivation amounts, and addition is the 0.6% of bacterium solution cultivation amount, goes out
Live time is that clostridium chauvei 38 DEG C inactivates 72～96 hours, and clostridium septicum 37 DEG C inactivates 100 hours, and bacillus perfringens inactivates
The detoxification time is respectively 37 DEG C 150 hours, and every day fully vibrates or stirs.
(4) inactivation check sampling, the lonely liver bouillon of inoculation is tested, and answers asepsis growth.
(5) detoxification inspection each 5 of each bacterium solution centrifuged supernatant intravenous injection body weight 18g white mice, every 0.5ml, sees
Examining 24 hours, mouse survival is qualified for inactivation detoxification.
4. concentrate
By gentle to inactivation inspection, the detoxification qualified clostridium septicum of inspection, C. perfringens Type C, bacillus perfringens D type
Swollen cellulitis clostridium bacterium solution is centrifuged, and centrifugal rotational speed is 8000r/min, centrifugal 20min, collects bacterial sediment and the bacterium of four kinds of bacterium solution respectively
Liquid supernatant.Collect clostridium septicum and clostridium chauvei bacterial sediment is put 2～8 DEG C and saved backup, c-type bacillus perfringens and D type
Bacillus perfringens bacterium solution supernatant ultrafilter membrane bag carries out being concentrated by ultrafiltration to 20,000ml, bacterium solution supernatant concentrated solution 2～8 DEG C of preservations
Standby.
5. join Seedling
Clostridium septicum and clostridium chauvei bacterial sediment are respectively with 20, and 000ml physiological saline solution suspends, with aseptic hydrogen
Sodium hydroxide solution adjustment bacterium solution pH value is to 6.5～7.5, with c-type bacillus perfringens and the bacterium solution supernatant of D type bacillus perfringens
Four kinds of composition mixed in equal amounts of concentrated solution, in bacterium solution 5 parts add aluminium glue 1 part ratio add sterilizing aluminium hydroxide gel make tetrad
Seedling.
Embodiment 2
Vaccine safety is tested
(1) clostridium septicum, C. perfringens Type C and bacillus perfringens D type three component
Vaccination 2kg rabbit 5, every subcutaneous injection 2ml (being equivalent to 5 part sheep immunizing doses) will be prepared, see
Examining 8,5 rabbit are all strong alive.
(2) blackleg part
With body weight 400g Cavia porcellus 2, each subcutaneous injection vaccine 2ml (is equivalent to 2 sheep immunizing doses), observes 8,5
Cavia porcellus is all strong to live.
Result above shows, ruminant clostridial disease tetrad inactivated vaccine prepared by the present invention meets the mark of safety verification
Accurate.
Embodiment 3
Vaccine potency test
(1) clostridium septicum, c-type bacillus perfringens and D type bacillus perfringens part
1) with the rabbit of 30 body weight 2kg, it is divided into 6 groups, often group 5；
2) 1～3 group carries out subcutaneous injection immunity, and immunizing dose is 0.5ml, and 4～6 groups the most immune, as a control group；
4) after immune 21 days, the clostridium septicum bacterium solution of 1,4 groups of injections 0.1ml (minimum lethal dose), 2,5 groups of injection 0.1ml
The c-type clostridium perfringens toxoid of (minimum lethal dose), the D type of 3,6 groups of injection 0.1ml (minimum lethal dose) minimum lethal doses produces
Gas capsular clostridium element poison.
5) observe 5.
6) result judges that comparison rabbit is the most dead, and immunity rabbit at least protects 4 to be qualified, the results are shown in Table 3.
Table 3 efficacy test results
(2) clostridium chauvei part
1) with the Cavia porcellus 10 of body weight 400g, it is divided into two groups, often group 5；
2) 1 group every intramuscular injection 1ml vaccine, 2 groups the most immune as a control group；
3) after immune 21 days, to two groups of Cavia porcelluss, 24 hours clostridium chauvei bacterium solution 0.2ml that intramuscular injection are cultivated respectively；4)
Observe 10.
Result is as follows: 1 group of survival rate be 5/5,2 group of survival rate be 0/5.
Result above shows, ruminant clostridial disease tetrad inactivated vaccine prepared by the present invention meets the mark of efficacy test
Accurate.Embodiment 4
Vaccine immunity method
Cattle intramuscular injection 2ml
Sheep intramuscular injection 1ml
Recommending to carry out two to exempt from, annual booster immunization is once.
Claims (3)
1. a ruminant clostridial disease tetrad inactivated vaccine, it is characterised in that this vaccine Main Ingredients and Appearance has clostridium chauvei and corruption
Lose the inactivated bacterial liquid of clostridium, c-type bacillus perfringens toxoid and D type bacillus perfringens toxoid.
2. a kind of ruminant clostridial disease tetrad inactivated vaccine as claimed in claim 1, it is characterised in that described tetrad inactivates
The seedling production bacterial strain of vaccine is clostridium septicum C55-1 strain, c-type bacillus perfringens C59-2 strain, D type bacillus perfringens
C60-2 strain and clostridium chauvei C54-1 strain.
The preparation method of a kind of ruminant clostridial disease tetrad inactivated vaccine the most as claimed in claim 1 or 2, it is characterised in that
Described vaccine preparation method is:
(1) described clostridium chauvei production bacterial strain bacterium solution inoculation appropriate media is cultivated, receive after inactivation, concentration
Collection concentrates bacterium solution；C-type bacillus perfringens and D type bacillus perfringens and clostridium septicum production bacterial strain bacterium solution are inoculated respectively
Appropriate media is cultivated, and collects preparation detoxification and concentrate bacterium solution after inactivation detoxification, concentration；
(2) ruminant clostridial disease four is obtained after adding adjuvant mixing after four kinds of concentration bacterium solution made above being mixed in proportion
Connection inactivated vaccine.
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| CN107308445A (en) * | 2017-07-26 | 2017-11-03 | 山东省滨州畜牧兽医研究院 | Anti- subunit vaccine of sheep three or four and preparation method thereof |
| CN108578686A (en) * | 2018-04-23 | 2018-09-28 | 武汉中拓康明生物科技有限公司 | A method of preparing clostridiosis of sheep genetic engineering subunit vaccine |
| CN111733094A (en) * | 2020-05-29 | 2020-10-02 | 内蒙古民族大学 | Bovine-derived bacillus cereus and application thereof |
| CN112107681A (en) * | 2020-10-28 | 2020-12-22 | 重庆澳龙生物制品有限公司 | Triple four-prevention inactivated vaccine for fast plague, sudden sniper, lamb dysentery and enterotoxemia and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107308445A (en) * | 2017-07-26 | 2017-11-03 | 山东省滨州畜牧兽医研究院 | Anti- subunit vaccine of sheep three or four and preparation method thereof |
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| CN111733094A (en) * | 2020-05-29 | 2020-10-02 | 内蒙古民族大学 | Bovine-derived bacillus cereus and application thereof |
| CN111733094B (en) * | 2020-05-29 | 2022-09-09 | 内蒙古民族大学 | Bovine-derived bacillus cereus and application thereof |
| CN112107681A (en) * | 2020-10-28 | 2020-12-22 | 重庆澳龙生物制品有限公司 | Triple four-prevention inactivated vaccine for fast plague, sudden sniper, lamb dysentery and enterotoxemia and preparation method thereof |
| CN112107681B (en) * | 2020-10-28 | 2021-11-12 | 重庆澳龙生物制品有限公司 | Triple four-prevention inactivated vaccine for fast plague, sudden sniper, lamb dysentery and enterotoxemia and preparation method thereof |
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