CN106176780B - A kind of new opplication of Gastrodin - Google Patents

Abstract

The invention discloses a kind of new opplications of Gastrodin；The Gastrodin is preparing the application in preventing/treating hepatic ischemia-reperfusion injury drug.The present invention has carried out internal pharmacodynamic study to Gastrodin, and experimental result is shown, Gastrodin can reduce rat model serum lipid overoxidation product assay, has stronger antioxidant activity；There is obvious inhibiting effect to the release of inflammatory factor；With inhibition liver inner cell apoptosis activity；Energy apparent increase NO concentration improves Microcirculation of Liver, has obvious protective function to hepatic ischemia-reperfusion injury.Gastrodin of the present invention can directly be used alone, and can also be combined with each other application, can include that plant extracts composition compound form is used with other drugs, can also use in a usual manner.

Description

A kind of new opplication of Gastrodin

Technical field

The invention belongs to pharmaceutical technology fields, and in particular to a kind of new opplication of Gastrodin.

Background technique

Tissue or organ will cause damage after certain time ischemic, cannot not only make its function after recovered blood perfusion It can restore with structure, make damage that referred to as postischemic reperfusion damage (ischemia-reperfusion be further aggravated instead injury ,I/RI).There is stream in a large amount of Ca2+ after Reperfu- sion, and generate a large amount of oxygen radicals, is extensive tissue cell insult Main pathogenesis.Clinically a variety of diseases such as Dalayed neuronal necrosis, irreversible shock, myocardial infarction, acute internal organs Generation, the development of functional failure and organ transplant rejection etc. are all related with ischemia-reperfusion.

Hepatic ischemia-reperfusion injury (HIRI) is the common pathological state of surgery of liver.Since the anatomical structure of liver is multiple Miscellaneous, blood supply is abundant, need to often block hepatic blood supply in liver surgery to reduce intraoperative hemorrhage.But after liver blood flow blocks HIRI is also resulted in while reducing bleeding.The damage often betides liver cancer excision, liver transfer operation, serious wound and infection etc. Pathologic process.HIRI can lead to liver dysfunction, and serious person can cause hepatic failure, or even dead.It is current research shows that HIRI Pathogenesis is not still fully aware of, may have following factor to participate in: oxidativestress damage, inflammatory cell infiltration, calcium overload, enzyme Effect, hepatocellular apoptosis etc..As the improvement HIRI aspect that is embedded in of research has remarkable progress, but effect is still undesirable. HIRI is the major reason for causing postoperative Primary graft failure, leading to liver transfer operation failure.Therefore how to mitigate HIRI is Surgery of liver especially spare-part surgery thorny problem urgently to be solved at present!

People generally acknowledge that HIRI is the result of many different mechanism interactions at present, therefore its prevention and treatment is also just different Method.Common method mainly has Ischemic reperfusion (IP) and medical preconditioning.(Gong NQ, Ye QF, Jang HY, et a.l P rotection of grafts by m ed icin e p recondi tioning and Ischem ia p recondit ion ing in liver transp lan tation [ J]. C hin JH epa Su rg, 2000, 6:282- 284.) however, this protective effect of liver IP is limited after all, (S chu lz R, the W alz such as Schu lz MK, Beh rends M, et a.l M in im al p rotection of the liver by ischem ic precond ition ing in pigs [ J] . Am J Phys iol Heart C irc Physiol, 2001, 198-H207. of 280:H) find that IP protective effect can maintain 1~2 h, IP is acted on after undergoing 200min long period ischemic It disappears, therefore limits it in clinical application.For how to improve the self supporting capacity of liver in liver surgery, It blocks liver blood flow to there are problems that HIRI, transfers its endogenous protection mechanism, mitigate liver I/RI, be facing for surgery of liver Bed demand.Ischemic reperfusion is implemented relatively more limited in liver transplantation, therefore IP and mould are induced with medical preconditioning The Delayed Protection of quasi- IPC directly protects the liver after I/RI, has wide potential applicability in clinical practice, is worth into one Step research.

Medical preconditioning is to reach similar Ischemic reperfusion using the direct or indirect pharmacological action of certain active materials Protective effect, enhance tissue or cell to the tolerance of ischemical reperfusion injury, to mitigate damage.Have to HIRI pre- The drug for the treatment of effect mainly has calcium channel blocker, free radical scavenger, the drug for improving microcirculation or cellular energy metabolism Deng.But many drugs have biggish toxic side effect, affect its application clinically, it is difficult to meet clinical needs. Therefore, developing a kind of drug that can solve above-mentioned problem is very important.

Summary of the invention

The purpose of the present invention is to provide a kind of new opplications of Gastrodin.

The object of the present invention is achieved like this, and the Gastrodin is preparing preventing/treating hepatic ischemia reperfusion damage Application in vulnerary object.

Rhizoma Gastrodiae (Gastrodia elata.BL) is the dry tuber of orchid Rhizoma Gastrodiae, is rare Chinese medicine, property is sweet, It is flat, return liver warp.Chinese medicine thinks that its primary efficacy has relieving convulsion and spasm, suppressing liver-YANG, dispelling wind and removing obstruction in the meridians.The chemistry extracted from Rhizoma Gastrodiae Ingredient has Gastrodine, gastrodia elata genin, vanillyl alcohol, vanillin, β-steroid paddy alcohol, daucosterol etc., and wherein active component content is most High effective monomer is Gastrodine, the entitled p- methylol benzene-β-D- glucopyranoside semihydrate (β-D-Gluc of chemistry Opyranoside), also known as Gastrodin.Modern pharmacology research shows that Gastrodin, which has, increases center and peripheral arterial blood vessel Compliance reduces peripheral vascular resistance, increases cardiovascular and cerebrovascular blood flow, generates mild antihypertensive effect, and to cardiac muscle cell, brain Tissue has protective effect, while having the effects that calmness, hypnosis, analgesia, enhancing are immune, is clinically widely used in the treatment heart The cerebrovascular, microcirculqtory system disease, it is significant in efficacy, and non-evident effect.Common dosage forms dosage form has Gastrodin on the market at present Injection, gastrodini, Rhizoma Gastrodiae cellulose capsule.

Gastrodin (i.e. to methylol benzene-β-D- glucopyranoside) is one of the effective monomer component of Rhizoma Gastrodiae.Modern medicine Reason studies have shown that Gastrodin has calm, analgesia, soporific function, also can anticonvulsion, antianxiety, can increase cerebral blood flow (CBF), subtract Few vascular resistence improves vertebral-basilar artery insufficiency, has neuroprotection, (brings up the medicine of Gui Chun Rhizoma Gastrodiae and its preparation Reason effect and clinical application research progress (J) China medicine company, 2008,17 (1): 64-66.) animal experiment study display Gastrodin The Apoptosis that cerebral ischemia can be inhibited to induce has neuroprotection, thin to the rat brain micro blood vessel endothelium of in vitro culture Born of the same parents' (BMEC) ischemic injuries have certain protective effect.(Yang Jie, Zhao Chaohua, Zong Changhong wait transience MCAO rat Injecting Gastrodin has neuroprotection (J) Journal of the Fourth Military Medical University, 2008,29 (4): 295-297.；Hu Jinghong, In Si Yinchu, protective effect (J) of flood celebrating great waves Gastrodine on Cultivated Rat Brain Microvessel Endothelial Cells by Mimic Cerebral Ischemia Central China medical magazine (former Chinese Medicine journal), 2007,22 (2): 124-126.； Zeng X H.Zhang Y , Zhang S M , etal A microdialysis is study of effect s of gast rodin on neurochemical changes in the ischemic/ reperfused ret cerebral hippocampus (J) .Biol Pham Bull, 2007,30 (4): 801.) research shows that Gastrodin, which also has, promotees intelligence anti-aging effects.(king Clear monarch practises Yang Yanbin, influence (J) solution of the Liu Jia Gastrodin to senescence-acceleratad mice cerebral tissue Aging-associated gene expression Cuing open scientific advance, 2007,13 (4): 353-357.) foreign study shows that Gastrodin is effective anxiolytic.(Jung JW, Yoon BH , Oh HR.et al.Anxiolvtie-like ef fects of Gast rodia elata and it S phenolie const ituents in mice (J) .Biol Pharm Bull, 2006,29 (2): 261-265.) Gastrodin is clinically widely used, treatment dizziness, vertebrobasilar insufficiency, cerebral arterial thrombosis, the headache of blood vessel character Etc. diseases, significant in efficacy, for hypertension, the adjuvant treatment of diabete peripheral herve pathology, effect affirmative is worth clinical weight Depending on.

Existing research shows that Gastrodin can enhance the compliance of blood vessel, improves internal organs anaerobic condition, is supplied by brain some Central nervous system disease caused by blood is insufficient has preferable therapeutic effect.Existing research at present show Gastrodin to brain and Heart ischemia reperfusion damage has protective effect, but there is not been reported to the effect of hepatic ischemia-reperfusion injury.

The present invention has carried out internal pharmacodynamic study to Gastrodin.Experimental result shows that Gastrodin can reduce rat model Serum lipid overoxidation product assay has stronger antioxidant activity；There is obvious inhibiting effect to the release of inflammatory factor； With inhibition liver inner cell apoptosis activity；Energy apparent increase NO concentration improves Microcirculation of Liver, to hepatic ischemia-reperfusion injury There is obvious protective function.

Gastrodin of the present invention can directly be used alone, and can also be combined with each other application, can include plant with other drugs Extract forms compound form and uses, and can also use in a usual manner.

Specific embodiment

Below with reference to embodiment, the present invention is further illustrated, but the present invention is limited in any way, Based on present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.

The new opplication of Gastrodin of the present invention is that the Gastrodin is preparing preventing/treating hepatic ischemia reperfusion Application in damage medicine.

Gastrodin preparation in the new opplication of the Gastrodin is pharmaceutically acceptable to be added in the Gastrodin Tablet, granule, capsule, pill, suspension or injection is made in auxiliary material.

With specific embodiment, the present invention will be further described below:

Embodiment 1

Influence of the Gastrodin to hepatic ischemia-reperfusion injury in rats:

1, reagent and animal

Gastrodin, content 98.8%, lot number: JT20161014, Kun Yao group production.

Male SD rat 40,250-300g is provided, production licence by Kunming Medical University's Experimental Animal Center: The Yunnan SCXK() K2015-0002, licence issuing authority: Kunming Office of Science and Technology.20 ~ 25 DEG C of (temperature difference per day≤3 of experimental animal room temperature DEG C), humidity 40% ~ 70%, illumination 12h:12h light and shade alternating, 150 ~ 300lx of illumination, noise≤60 Db；Experimental animal use is permitted Can demonstrate,prove: the Yunnan SYXK() K2014-0001, issuing unit: Kunming Office of Science and Technology.

Malonaldehyde (MDA) testing cassete, superoxide dismutase (SOD) testing cassete, glutamic-oxalacetic transaminease (AST) testing cassete, paddy Pyruvic transaminase (ALT), glutathione (GSH) testing cassete, superoxide dismutase (SOD), glutathione (GSH), malonaldehyde (MDA) kit builds up biological reagent company purchased from Nanjing；TUNEL kit: source: Roche, lot number: 10348000；It is anti- Cleaved Caspase-3 antibody, Cell Signaling Technology, lot number: 19；Alexa Fluor568 donkey anti-rabbit IgG (H+L), Molecular Probes, lot number: 1134929；Alexa Fluor568 goat anti rat IgG (H+L), Molecular Probes, lot number: 1259374；Triton X-100, Sigma, lot number: 031M0301V；The examination of BCA protein quantification Agent box, Shanghai Wei Ao Biotechnology Co., Ltd, lot number: WB0123；Interleukin-11 β (IL-1 β): lot number DZE30419；Interleukin 6(IL-6): lot number DZE30646；Tumor necrosis factor α (TNF-α): lot number DZE30635；The above reagent is purchased from Shanghai and breathes out spirit Biotechnology Co., Ltd.

2, experimental method

2.1 animal models and grouping

Experimental animal is divided into sham-operation group, liver I/RI model group (model group) and Gastrodin pretreatment low dosage at random With high dose group (60,120mg/kg)+liver I/RI model group (Gastrodin pre-processes low, high dose group).Preoperative 12 h fasting, Free water.With 1% yellow Jackets (30 mg/kg) solution intraperitoneal injection of anesthesia rat, about 2 cm of median abdominal incision is taken Enter abdomen, dissociate ligamentum hepatoduodenale, fills again after closing 30 min of portal vein, arteria hepatica and choledochus with not damaged artery clamp folder 6 h of note makes hepatic ischemia reperfusion animal model, and (Zhao Haifeng, Kong Rui, Sun Bei wait Sodium butyrate to rat liver heat General surgery of research [J] the China magazine of ischemical reperfusion injury protective effect, 2008,17 (1): 91-93.).Artificial hand Art group dissociates ligamentum hepatoduodenale without blocking, and preoperative 20 min of Gastrodin pretreated group passes through tail vein injection Gastrodin (60,120mg/kg), model group gives same amount of normal saline.Each group rat is after postoperative 6 h from inferior caval vein blood sampling about 5 mL；Left lobe of liver is cut, remainder splits -80 DEG C and freezes, and hepatic tissue biochemical indicator and caspase-3 to be measured are horizontal.

2.2 observation index and detection method

(1) liver functional testing:

Serum is taken after the centrifugation of rat vein blood, detects glutamic-oxalacetic transaminease (AST) with respective kit method, third turn of ammonia of paddy Enzyme (ALT) is horizontal.

(2) hepatic tissue biochemical indicator detects:

It is horizontal with the detection of respective kit method hepatic tissue SOD, GSH and MDA.

(3) hepatic tissue caspase-3 level detects:

12-16h will be fixed in the paraformaldehyde solution of rat liver merging 4%.The liver fixed is carried out frost to cut Piece is placed in and freezes in protection liquid, and -20 C are saved backup, to carry out Cleaved Caspase-3 immunohistochemical staining, TUNEL dyeing.Frozen section is placed in 0.3%Triton X-100 30min after 0.01M PBS rinsing, afterwards directly with corresponding one It is anti-to be incubated for (4 C are stayed overnight).Slice is with PBS rinsing 3 times, every time 5min, then reacts at room temperature 1-2h with corresponding fluorescence secondary antibody, subsequent It is continuous to be rinsed with PBS, and mounting is carried out with mountant.Coloration result is taken pictures through Nikon fluorescence microscope, and with Adobe Photoshop analysis；It is carried out by the detection specification of In situ cell apoptosis appended by kit.Specifically, frozen section first with PBS rinsing, is then incubated for, then directly with TUNEL reaction solution (by marking fluid and enzyme reaction solution group with 0.1%TritonX-100 At) be incubated for, 60min is protected from light under the conditions of 37 degree.Coloration result is directly observed and is taken pictures under fluorescence microscope.

(4) inflammatory factor detects:

Serum is taken after the centrifugation of rat vein blood, is operated in strict accordance with kit specification, IL-1, IL-6, TNF-α are measured.

2.3 statistical procedures

Data with average value and standard deviation (± SD) it indicates.Statistical analysis uses one-way analysis of variance, comparison among groups It tests analysis using student Newman-Keul ' s test.Think with statistical difference P < 0.05.

3, result

The horizontal testing result of 3.1 Serum ALTs and AST

Compared with sham-operation group, model group rats Serum ALT and AST horizontal significantly raised (equal P < 0.05), and with Model group compares, and the horizontal obvious attenuating (equal P < 0.05) of the Serum ALT and AST of Gastrodin pretreated group is shown in Table 1.

1 each group rat blood serum transaminase level of table compare (± SD), n=10

Group	ALT(U/L)	AST(U/L)
			Sham-operation group	44.5±4.6	90.1±9.1
Model group	85.7±6.1*	234.3±16.3*
			Gastrodin pre-processes low dose group	69.3±5.3▲	163.1±11.4▲
Gastrodin pre-processes high dose group	61.2±4.0▲	140.5±13.8▲▲

Note: compared with sham-operation group, * P < 0.05, * * P < 0.01；Compared with model group, ▲ P < 0.05, ▲ ▲ P < 0.01.

3.2 biochemical indicator testing results

Compared with sham-operation group, SOD and GSH level is substantially reduced (P < 0.05) in model group hepatic tissue, and MDA Horizontal apparent increase (P < 0.01).Compared with model group, the horizontal significant liter of SOD and GSH in Gastrodin pretreated group hepatic tissue Height, MDA level significantly reduce, and (P < 0.05 or P < 0.01) is shown in Table 2.

2 each Biochemical Indices In Serum of each group rat of table comparison (± SD), n=10

Group	SOD(U/mg albumen)	GSH(mg/mg albumen)	MDA(nmol/mg albumen)
				Sham-operation group	305.1±16.2	3.13±0.20	1.98±0.57
Model group	204.2±34.5*	1.88±0.75*	4.67±0.89**
				Gastrodin pretreatment is low	286.5±15.2▲	2.64±0.13▲	3.56±0.72▲
Gastrodin pretreatment is high	377±12.4▲▲	3.76±0.65▲▲	2.09±0.47▲▲

Note: compared with sham-operation group, * P < 0.05, * * P < 0.01；Compared with model group, ▲ P < 0.05, ▲ ▲ P < 0.01.

And the mechanism of hepatic ischemia-reperfusion injury and formation, inflammatory cell infiltration, hepatocellular apoptosis of oxygen radical etc. Many factors are related.(Kang KJ. Mechanism of hepatic ischemia/reperfusion injury and protection against reperfusion injuryl[J]. Transplant Proc, 2002, 4(7):2659- 2661.) this result of study is shown, 30 min of hepatic ischemia/reperfusion injury, in the Ischemia and Reperfusion in vivo in Rats model of 6 h of Reperfu- sion, gives Rhizoma Gastrodiae Element can be substantially reduced Serum ALT and AST is horizontal (P < 0.05), mitigate hepatic pathology damage.These results prompt Gastrodin pre- Processing has protective effect to liver I/RI.There is research to also confirm that be overexpressed SOD in liver I/RI animal model or give GSH, (He SQ, Zhang YH, Venugopal SK, et al. Delivery of antioxidative enzyme genes protects against ischemia/reperfusion-induced liver injury in mice[J]. Liver Transpl, 2006, 12(12):1869-1879.； Schauer RJ, Gerbes AL, Vonier D, et al. Glutathione protects the rat liver against reperfusion injury after Prolonged warm ischemia [J] Ann Surg, 2004,239 (2): 220-231.) or the base removing that freers Agent, as Edaravone can mitigate liver I/RI.(Shi Chenlei, Qin Huadong, stone iron cutting edge of a knife or a sword Edaravone is to rats'liver I/RI General surgery of protective effect [J] the China magazine of damage, 2010,19 (1): 28-31.)

The display of this experimental result, after giving Gastrodin, the horizontal obvious liter of SOD and GSH in the hepatic tissue of ischemia-reperfusion It is high；It is significantly reduced simultaneously as the MDA level of products of oxidative stress, shows that ischemia-reperfusion liver can be improved in Gastrodin The oxidation resistance of tissue.

The influence of 3.3 pairs of Apoptosis

In our current research, we have detected apoptotic cell (TUNE method) and signaling molecule relevant to apoptosis (such as Caspase-3)。

As shown in table 3,30 min of hepatic ischemia/reperfusion injury, in the Ischemia and Reperfusion in vivo in Rats model of 6 h of Reperfu- sion, intracerebral TUNEL method The apoptotic cell and Cleaved Caspase-3 immuno positive (Cleaved Caspase-3-ir) cell of dye marker go out Now increase (compared with sham-operation group, P < 0.01), can then significantly reduce intracerebral TUNEL method dye marker after giving Gastrodin Apoptotic cell and Cleaved Caspase-3 immuno positive (Cleaved Caspase-3-ir) cell (compared with model group, P < 0.05 or P < 0.01).

3 Gastrodin of table to Apoptosis influence (± SD), n=10

Note: compared with sham-operation group, * P < 0.05, * * P < 0.01；Compared with model group, ▲ P < 0.05, ▲ ▲ P < 0.01.

Hepatocellular apoptosis is a major reason of hepar damnification in liver I/RI, and during Apoptosis The activation of caspase-3 is most important link, and wherein caspase-3 is most important apoptosis executor.Caspase-3's Activation can directly or indirectly act on digestion (Porter AG, the J nicke RU. of key protein in apoptosis implementation procedure Emerging roles of caspase-3 in apoptosis[J]. Cell Death Differ, 1999, 6(2): 99-104.).This experiment shows that in the animal model of liver I/RI, Gastrodin can obviously inhibit TUNEL method in rats'liver The apoptotic cell and Cleaved Caspase-3 immuno positive (Cleaved Caspase-3-ir) cell of dye marker, can Mitigate liver I/RI, prompts Gastrodin that can inhibit the apoptosis of liver cell after ischemia-reperfusion.

The influence of 3.4 pairs of inflammatory factors

After Reperfu- sion 6h, compared with sham-operation group, model group Serum TNF-α, IL-1 β and NO it is horizontal it is significantly raised ( P < 0.05 or P < 0.01), and NO horizontal significantly raised (P < 0.05)；Compared with model group, Gastrodin pretreated group TNF-α and IL-1 β level significantly reduces, and the horizontal significant raising (P < 0.05 or P < 0.01) of NO, is shown in Table 4.

Table 4 to inflammatory factor influence (± SD, n=10)

Note: compared with sham-operation group, * P < 0.05, * * P < 0.01；Compared with model group, ▲ P < 0.05, ▲ ▲ P < 0.01.

This Germicidal efficacy arrives, after Reperfu- sion: the Serum ALT and AST level of Gastrodin pretreated group are significantly lower than model Group；SOD and GSH level is significantly higher than model group, and serum MDA level is obviously reduced compared with model group.It also found liver Reperfu- sion Serum TNF-α and IL-1 inflammatory factor level increase afterwards, and the release of Gastrodin pretreated group Serum TNF-α and IL-1 is pressed down System, the index of reperfusion injury also accordingly decline, and mitigate liver reperfusion injury by reducing TNF-α and IL-1 release.Ba The TNF-α of jt etc. [3] discovery Kupffer Cell secretion can form TNFR-TRADD-FADD in conjunction with the TNFR1 on liver plasma membrane Compound simultaneously raises Caspase-3 gene expression, the cascade reaction of activation C aspase family, active cell apoptosis, I Further with TUNEL method detection Reperfu- sion after liver cell apoptosis the case where, as a result, it has been found that the cell of Gastrodin pretreated group Apoptosis is significantly inhibited, and shows that Gastrodin can inhibit liver cell apoptosis, and this effect may be by inhibiting withered no Cell-stimulating, the release for reducing TNF-α are realized.It has been reported that the microcirculation disorders of early stage are mainly with NO's after liver Reperfu- sion Produce related, it is a major reason of microcirculation disorder after hepatic ischemia reperfusion that the concentration of NO is unbalance.We also observe NO level changes after liver Reperfu- sion, the horizontal significant raising of the NO after discovery Reperfu- sion in blood, Gastrodin pretreated group NO water Flat elevation amplitude is big compared with model group, illustrates that Gastrodin can be by adjusting NO concentration ratio, liver is micro- after improvement Reperfu- sion Circulation, to play protective effect.

To sum up, Gastrodin can mitigate hepatic ischemia-reperfusion injury in rats, and possible mechanism of action includes reducing oxygen freedom The generation of base inhibits inflammatory cytokine to discharge, microcirculation etc. after reduction liver cell apoptosis and improvement Reperfu- sion.

The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (2)

1. a kind of application of Gastrodin, it is characterised in that the Gastrodin is in preparation energy elevation of NO concentration with preventing/treating liver Application in ischemical reperfusion injury drug.

2. the application of Gastrodin according to claim 1, it is characterised in that being added in the Gastrodin can pharmaceutically connect Tablet, granule, capsule, pill, suspension or injection is made in the auxiliary material received.