CN106166297A - A kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth - Google Patents
A kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth Download PDFInfo
- Publication number
- CN106166297A CN106166297A CN201610725858.3A CN201610725858A CN106166297A CN 106166297 A CN106166297 A CN 106166297A CN 201610725858 A CN201610725858 A CN 201610725858A CN 106166297 A CN106166297 A CN 106166297A
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- China
- Prior art keywords
- hydrochloric acid
- oral liquid
- bacterial growth
- veterinary
- fist
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/02—Inorganic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Inorganic Chemistry (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth, take three taste fist god oral liquid 500ml, using pH meter to measure pH is 7.2, is slowly added to hydrochloric acid, starts to produce precipitation to pH=2.9, and regulation pH is 3,.When the method that the present invention provides uses the pH to 3 that hydrochloric acid reduces oral liquid, its antibacterial effect reaches optimal；After acidifying, the use hydrochloric acid identical with gastric acid has no side effect after can ensure that and taking, preserved by physical method to keep the composition of oral liquid unaffected simultaneously, reduce the harm that animal may be caused by preservative, can not affected by gastric acid with security function oral liquid after being simultaneously introduced hydrochloric acid, preferably be kept the activity of effective ingredient.
Description
Technical field
The invention belongs to veterinary oral liquid corrosion-resistant field, particularly relate to a kind of by hydrochloric acid suppression veterinary oral liquid antibacterial
The method of growth.
Background technology
The raising recognized food safety along with people, veterinary drug application in animal epidemic is prevented and treated the most gradually increases.Beast
The dosage form of medicine has powder, tablet, granule, tincture, injection, oral liquid etc. on veterinary drug market.And oral liquid is offerd medicine because of it
Convenient, not only it was easy to group anti-but also facilitate individual treatment, simultaneously because eliminate medical material suck tissue and partial invalidity composition carries accordingly
The high concentration of effective ingredient, decreases consumption, has preferably played the effect of medicine, and welcome by numerous raisers.
Oral liquid is many with water as solvent, is limited by existing working condition and the shadow of the factors such as the cleanliness factor of raw material of Chinese medicine own
Ring, the easy mildew of medicine and fermentation and there is qualitative variability.Particularly, veterinary oral liquid is typically Chinese medicine, often contains in Chinese medicine
Substantial amounts of polysaccharide, protein, aminoacid isoreactivity material, these materials contain a large amount of applicable antibacterial, the nutrient of fungus growth
Matter, conventional art prevents bacterial growth, common preservative from having an alcohols using preservative: ethanol and benzyl alcohol；Benzoic acid and
Its salt, sorbic acid and salt thereof, parabens etc..But the use of preservative is often subject to the impact of various factors.Such as, conventional
Preservative many genus weak organic acid compounds, this kind of preservative works with molecule-type, and the pH value of solution is to preservative
Degree of dissociation has an impact, thus affects fungistatic effect.And the input method of some preservative is different, its inhibitor effectiveness is also by shadow
Ring.
Antiseptic and inhibiting bacteria function effect in view of existing preservative is affected by various factors, and the present invention provides one to make
Reduce the pH of oral liquid with hydrochloric acid thus reach the condition of optimal bacteria growing inhibiting, thus reduce the use of preservative.
Summary of the invention
For the deficiencies in the prior art, the purpose of the present invention, it is provided that a kind of raw by hydrochloric acid suppression veterinary oral liquid antibacterial
Long method, can reach optimal inhibition, effectively reduce the use of preservative simultaneously.
The technical scheme is that
A kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth, takes three taste fist god oral liquid 500ml, uses pH meter to survey
Determining pH is 7.2, is slowly added to hydrochloric acid, starts to produce precipitation to pH=2.9, and regulation pH is 3,.
A kind of above-mentioned method by hydrochloric acid suppression veterinary oral liquid bacterial growth, preferred scheme is, described hydrochloric acid
Concentration is 1mol/L.
A kind of above-mentioned method by hydrochloric acid suppression veterinary oral liquid bacterial growth, preferred scheme is, that is suppressed is thin
Bacterium includes avian pasteurella multocida, Staphylococcus gallinarum, Pullorum Disease salmonella, Klebsiella Pneumoniae, escherichia coli etc..
The excellent results of the present invention is also manifested by:
(1), when using the pH to 3 of hydrochloric acid reduction oral liquid, its antibacterial effect reaches optimal.
(2) after being acidified, the use hydrochloric acid identical with gastric acid has no side effect after can ensure that and taking, and passes through physical method simultaneously
Preservation can keep the composition of oral liquid unaffected, reduces the harm that animal may be caused by preservative, is simultaneously introduced salt
Can not affected by gastric acid with security function oral liquid after acid, preferably be kept the activity of effective ingredient.
Detailed description of the invention
Describe technical scheme in detail below in conjunction with embodiment and experimental example, but protection domain is not limited to this.
Three-drug adder-wort oral liquid: purchase and reach bio tech ltd in Qing Daorun, the every 1ml of product specification is equivalent to primary
Medicine 1g, 250ml/ bottle.
Embodiment 1: a kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth, takes three taste fist god's oral liquids
500ml, using pH meter to measure pH is 7.2, is slowly added to the hydrochloric acid that concentration is 1mol/L, starts to produce precipitation to pH=2.9, adjusts
Joint pH is 3,.Wherein, the antibacterial suppressed includes avian pasteurella multocida, Staphylococcus gallinarum, Pullorum Disease salmonella, pneumonia gram
The primary bacterium of thunder, escherichia coli etc..
Embodiment 2: a kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth, takes three taste fist god's oral liquids
500ml, using pH meter to measure pH is 7.2, is slowly added to the hydrochloric acid that concentration is 1mol/L, starts to produce precipitation to pH=2.9, adjusts
Joint pH is 4,.Wherein, the antibacterial suppressed includes avian pasteurella multocida, Staphylococcus gallinarum, Pullorum Disease salmonella, pneumonia gram
The primary bacterium of thunder, escherichia coli etc..
Embodiment 3: a kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth, takes three taste fist god's oral liquids
500ml, using pH meter to measure pH is 7.2, is slowly added to the hydrochloric acid that concentration is 1mol/L, starts to produce precipitation to pH=2.9, adjusts
Joint pH is 3.5,.Wherein, the antibacterial suppressed includes avian pasteurella multocida, Staphylococcus gallinarum, Pullorum Disease salmonella, pneumonia
Klebsiella, escherichia coli etc..
Test example processed by the inventive method after the experimental study of three taste fist god's oral liquids:
1. bacteriostatic test
1.1 subjects and method
The three taste fist each 1ml of god's oral liquid after embodiment of the present invention 1-3 processes pour incubated overnight survey in nutrient agar into
Determine bacterial content.
1.2 result of the test
Result is as shown in table 1.
The bacterial content of table 1 embodiment 1-3 gained three taste fist god's oral liquid
| pH | 3 | 3.5 | 4 |
| Total number of bacteria (cfu/ml) | 0 | 120 | 300 |
From the data in table 1, it can be seen that after adding the hydrochloric acid of 1mol/L in three taste fist god's oral liquids, fungistatic effect is had aobvious by the pH of solution
Write impact.When wherein pH is 3, its bacterial content is 0, and when pH is respectively 3.5 and 4, bacterial content apparently higher than pH be 3 time
Bacterial content.It is indicated above that three taste fist god's oral liquids of pH=3 do not have bacterial growth, and precipitation will not be produced.
2. effective constituent determination test:
Take respectively and add hydrochloric acid and be not added with the three-drug adder-wort oral liquid 10ml tri-parts of hydrochloric acid as parallel control, addition chloroform
25ml, shakes up, add strong ammonia solution regulation pH value to 9.5, be heated to reflux 1 hour, let cool.Taking chloroform liquid to be evaporated, residue adds
Ethanol 2ml makes dissolving, as need testing solution, separately takes oxymatrine reference substance, adds ethanol and makes every 1ml solution containing 1mg,
As reference substance solution.Test according to thin layer chromatography, with benzene-acetone and ethyl acetate-dense ammonia (2:3:4:0.2) as developing solvent, exhibition
Opening taking-up, dry, spray is with dilute bismuth potassium iodide test solution, in test sample chromatograph, on the position corresponding with reference substance chromatograph, shows phase
Same gray corrosion, it is indicated above that add the three-drug adder-wort oral liquid after hydrochloric acid, its effective ingredient is not affected.
Meanwhile, the gastric acid of animal is that hydrochloric acid, usually less than pH are less than 2, and the pH of vinegar is usually about 2.9, therefore pH=3
Hydrochloric acid animal bodies will not be worked the mischief, take safety.
It should be pointed out that, the detailed description of the invention more representational example that is the present invention, it is clear that the skill of the present invention
Art scheme is not limited to above-described embodiment, it is also possible to have many variations.Those of ordinary skill in the art, the most public with present invention institute
What what that open or according to file written description was undoubted obtained, all it is considered as this patent scope of the claimed.
Claims (3)
1. the method by hydrochloric acid suppression veterinary oral liquid bacterial growth, it is characterised in that take three taste fist god's oral liquids
500ml, using pH meter to measure pH is 7.2, is slowly added to hydrochloric acid, starts to produce precipitation to pH=2.9, and regulation pH is 3,.
Method the most according to claim 1, it is characterised in that the concentration of described hydrochloric acid is 1mol/L.
Method the most according to claim 1, it is characterised in that the antibacterial suppressed includes avian pasteurella multocida, chicken Fructus Vitis viniferae ball
Bacterium, Pullorum Disease salmonella, Klebsiella Pneumoniae, escherichia coli etc..
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610725858.3A CN106166297A (en) | 2016-08-26 | 2016-08-26 | A kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610725858.3A CN106166297A (en) | 2016-08-26 | 2016-08-26 | A kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106166297A true CN106166297A (en) | 2016-11-30 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610725858.3A Pending CN106166297A (en) | 2016-08-26 | 2016-08-26 | A kind of method by hydrochloric acid suppression veterinary oral liquid bacterial growth |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843198A (en) * | 2005-04-07 | 2006-10-11 | 卡夫食品集团公司 | Shelf-stable cold-processed food compositions and methods for their preparation |
| CN101849996A (en) * | 2010-06-08 | 2010-10-06 | 江西中成药业集团有限公司 | Three-drug adder-wort powder and preparation method thereof |
-
2016
- 2016-08-26 CN CN201610725858.3A patent/CN106166297A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843198A (en) * | 2005-04-07 | 2006-10-11 | 卡夫食品集团公司 | Shelf-stable cold-processed food compositions and methods for their preparation |
| CN101849996A (en) * | 2010-06-08 | 2010-10-06 | 江西中成药业集团有限公司 | Three-drug adder-wort powder and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 张秀美: "《禽病诊治实用技术》", 30 September 2002, 山东科学技术出版社 * |
| 湖北绿水动物药业有限公司: "三味拳参口服液", 《HTTP://WWW.1866.TV/PRO/398326.HTML#RETURN_PHONE》 * |
| 王珅，等: "《动物微生物》", 30 April 2007, 中国农业大学出版社 * |
| 王红宁: "《禽呼吸系统疾病》", 31 October 2002, 中国农业出版社 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161130 |
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| RJ01 | Rejection of invention patent application after publication |