CN106165647A - Red palm tissue-culturing rapid propagation inoculation method - Google Patents
Red palm tissue-culturing rapid propagation inoculation method Download PDFInfo
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- CN106165647A CN106165647A CN201610633137.XA CN201610633137A CN106165647A CN 106165647 A CN106165647 A CN 106165647A CN 201610633137 A CN201610633137 A CN 201610633137A CN 106165647 A CN106165647 A CN 106165647A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
Red palm tissue-culturing rapid propagation inoculation method designed by the present invention, it includes: 1, is peeled off from callus by the adventitious bud that anthurium andraeanum callus differentiation obtains, and is inoculated on elongation medium or root media or proliferated culture medium cultivation.Condition of culture is: temperature 25 DEG C, intensity of illumination 1500～2500Lux, illumination every day 15～17h, cultivates through 40～60d, it is thus achieved that healthy red palm tissue cultured seedling；From healthy red palm tissue cultured seedling, choose plant height 2cm and more than, and the health red palm tissue cultured seedling possessing 2 joints and above stipes, in inoculation dish, crosscutting at its Seedling stem stipes Section 1 top 2mm from bottom to top, form upper and lower two parts, upper part is inoculated in root media, lower part is inoculated in elongation medium continuation and cultivates, and condition of culture is identical with step 1, can obtain the red palm tissue cultured seedling that growth is neat.The present invention can improve the uneven problem of plant strain growth during red palm tissue-culturing rapid propagation.
Description
Technical field
The present invention relates to plant biotechnology field, in particular to a kind of red palm tissue-culturing rapid propagation inoculation method.
Technical background
The red palm (Anthurium andraeaum), has another name called fancy candles lit in the bridal chamber at wedding, An Zuhua or fire crane flower, many for Araeceae Anthurium
Nian Sheng evergreen herbage, originates in South America Tropical rain forest, and all there is extensively cultivation in Europe, Asia, Africa now.Its floral leaf is graceful
Elegance, flower pattern is unique, for leaf bract, has spadix, bright in colour magnificent, has great ornamental value, and the florescence is long,
Can bloom the whole year, Hua Yushi " newly-married, blessing, lucky, happy, ride on the crest of success, enthusiasm, warm blood ", become modern room and various
The decoration treasure that large-scale activity place is rare.
Within 2012, China's red palm landings are 15,220,000 basins, and within 2013, landings are more than 1,900 ten thousand basins, and ascensional range reaches
27.5%, high financial profit, market scale is huge.The propagation method of red palm routine is seed propagation and division propagation, but breeding
Coefficient is low, the cycle is long, it is difficult to meets the great demand of existing market, exists such as in addition: the scarcity of improved seeds；Genetic breeding
The research of aspect is less, and theoretical research is combined the tightst with production practices；Planting scale is little, yield per unit area, the output value
Ratio is relatively low；The problems such as sales section is weak, cause China's a large amount of high-quality red palm seedling dependence on import every year.
Plant tissue culture technique can provide a large amount of kind while ensureing maternal improved seeds characteristic at short notice
Seedling, becomes one of domestic red palm industry main path breaking away from dependence on import.
Red palm tissue culture technique is studied by lot of domestic and foreign researcher, and obtains certain progress, but due to
The foundation of red palm tissue culture regeneration system is relatively slow, and plant strain growth is uneven, and subculture number too much causes seedling to degenerate or becomes
The reason such as different, the tissue cultured seedling of domestic production and import seed there is also bigger gap.Therefore, it is necessary to grope and grasp efficient
High-quality red palm tissue culture sprout quick propagating technology, reduces subculture multiplication number of times while controlling tissue cultured seedling regularity, guarantee seedling amount as far as possible.
Currently, with respect to the existing more research report of the tissue-culturing rapid propagation culture medium prescription of the red palm and cultivation technique (Xia Shiyun,
Deng. improve the induction of red palm Callus of Leaf and Plantlet Differentiation and the technical research of rate in strong sprout. China's agronomy circular, 2005,21
(2):45-48；Zheng Xueping, etc. the different factor impacts on red palm growth coefficient. north gardening, 2009, (7): 65-68；Guo Yun
Expensive, wait the optimization of anthurium andraeanum callus proliferation and differentiation condition. China's gardening digest, 2012,5:10-12；Niu Ruihe, etc. the red palm
Callus culture and the research of adventitious buds differentiation influence factor. Agriculture of Anhui science, 2013,41 (24): 9914-9916；Cui
Rui Feng, etc. the research of red palm Callus of Leaf. Jiangsu's agriculture science, 2013,41 (9): 24-26 ...), but not yet retrieve
To about improve the most numerous during the report of inoculation method.
Summary of the invention
Present invention aim to provide a kind of red palm tissue-culturing rapid propagation inoculation method, the method can preferably improve red palm group
Train the most numerous during the uneven problem of plant strain growth, also can make up because of inoculation Subculture Time postpone formed too high tissue cultured seedling
The loss caused, meanwhile, can reduce callus differentiation adventitious bud number of times, reduce too much cause seedling to degenerate because of subculture number or
The risk of variation.
For realizing this purpose, the red palm tissue-culturing rapid propagation inoculation method designed by the present invention, it comprises the steps:
Step 1: the cultivation of red palm tissue cultured seedling；
The adventitious bud that anthurium andraeanum callus differentiation obtains is peeled off from callus, length is good for less than 0.5cm
Every 2～3 one group of health adventitious bud, after band callus cuts, is inoculated on proliferated culture medium cultivation, is more than or equal to for length
0.5cm but healthy adventitious bud less than 2cm is inoculated in elongation medium cultivation, for the length health more than or equal to 2cm not
Normal bud is inoculated on root media cultivation, and enrichment culture, elongation are cultivated and the condition of culture of root culture is: temperature 24～
26 DEG C, intensity of illumination 1500～2500Lux, illumination every day 15～17h, cultivate through 40～60d, it is thus achieved that healthy red palm group is trained
Seedling；
Step 2: the red numerous inoculation of palm tissue culture sprout quick；
From the health red palm tissue cultured seedling that step 1 obtains, choose plant height 2cm and more than, and possess 2 joints and above stipes
Healthy red palm tissue cultured seedling, in inoculation dish, on Seedling stem stipes Section 1 top from bottom to top of the health chosen red palm tissue cultured seedling
At 1.8～2.2mm crosscutting, form upper and lower two parts, upper part is inoculated in root media, lower part be inoculated in elongation cultivate
Continuing in base to cultivate, condition of culture is identical with step 1, can obtain the red palm tissue cultured seedling that growth is neat.
Beneficial effects of the present invention:
(1) present invention is simple and easy to do, easy to operate, can in longer time range manual control red palm tissue culture plant inoculation
Time, be conducive to more effectively arranging production according to personnel, order etc..
(2) it is also possible to apply the invention to, because inoculation Subculture Time postpones the too high tissue cultured seedling formed, breeding amount, more to be increased
Mend loss.
(3) present invention saves time, cost-effective, reduces callus proliferation subculture number simultaneously, thus reduces because planting
The risk that Seedling is degenerated or variation causes.
Accompanying drawing explanation
Fig. 1 a is that anthurium andraeanum callus adventitious bud inoculates preoperative schematic diagram；
Fig. 1 b is the schematic diagram after the inoculation operation of anthurium andraeanum callus adventitious bud；
Fig. 2 a is the red preoperative schematic diagram of palm tissue culture plant inoculation；
Fig. 2 b is the schematic diagram after the operation of red palm tissue culture plant inoculation；
Fig. 3 a is for cultivating regularity schematic diagram after carrying out red palm inoculation according to existing method；
Fig. 3 b is for cultivating regularity schematic diagram after carrying out red palm inoculation according to the present invention；
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail:
Red palm tissue-culturing rapid propagation inoculation method designed by the present invention, it comprises the steps:
Step 1: the cultivation of red palm tissue cultured seedling；
The adventitious bud that anthurium andraeanum callus differentiation obtains is peeled off from callus, length is good for less than 0.5cm
Every 2～3 one group of health adventitious bud, after band callus cuts, is inoculated on proliferated culture medium cultivation, is more than or equal to for length
0.5cm but healthy adventitious bud less than 2cm is inoculated in elongation medium cultivation, for the length health more than or equal to 2cm not
Normal bud is inoculated on root media cultivation, and enrichment culture, elongation are cultivated and the condition of culture of root culture is: temperature 24～
26 DEG C (preferably 25 DEG C), intensity of illumination 1500～2500Lux, illumination every day 15～17h (preferably 16h), through 40～60d trainings
Support, it is thus achieved that healthy red palm tissue cultured seedling；
Step 2: the red numerous inoculation of palm tissue culture sprout quick；
From the health red palm tissue cultured seedling that step 1 obtains, choose plant height 2cm and more than, and possess 2 joints and above stipes
Healthy red palm tissue cultured seedling, in inoculation dish, on Seedling stem stipes Section 1 top from bottom to top of the health chosen red palm tissue cultured seedling
1.8～2.2mm (preferably 2mm) place is crosscutting, forms upper and lower two parts, and upper part is inoculated in root media, and lower part is inoculated in
Continuing in elongation medium to cultivate, condition of culture is identical with step 1, can obtain the red palm tissue cultured seedling that growth is neat.
In technique scheme, described elongation medium is MS (Murashige and Skoog, lower same) minimal medium
With the material of additional different components, wherein accessory ingredients include 1.0～1.5mg/L 6-benzyladenine (6-BA), 0.05～
The sucrose (preferably 30g/L) of 2,4 dichlorophenoxyacetic acid (2,4-D), 28～the 32g/L of 0.2mg/L, 6～8g/L (preferably 7g/L)
Agar powder, the pH value of described elongation medium is 5.8.
In technique scheme, described root media is MS minimal medium and the material of additional different components, wherein
Accessory ingredients includes indolebutyric acid (IBA), 2～activated carbon, 28～the 32g/L of 3g/L (preferably 2.5g/L) of 0.5～1.0mg/L
The agar powder (preferably 7g/L) of sucrose, 6～the 8g/L of (preferably 30g/L), the pH value of described root media is 5.8.
In technique scheme, described proliferated culture medium is MS minimal medium and the material of additional different components, wherein
Accessory ingredients include 1.0～3.0mg/L the 2,4 dichlorophenoxyacetic acid of 6-benzyladenine, 0.1～0.5mg/L, 28～
The agar powder (preferably 7g/L) of sucrose (preferably 30g/L), 6～the 8g/L of 32g/L, the pH value of described proliferated culture medium is 5.8.
In technique scheme, the pH value of elongation medium, root media and proliferated culture medium is all by NaOH and HCl
Regulation.
In technique scheme, described 6-benzyladenine, 2,4-dichlorphenoxyacetic acid and indolebutyric acid all use 0.1mol/
The NaOH of L or 95% ethanol dissolve, then with being configured to the mother solution of 0.5mg/ml after aquae destillata dilution respectively, load in volumetric flask,
It is placed in 3～5 DEG C of (preferably 4 DEG C) Refrigerator stores.
In technique scheme, elongation medium, root media and proliferated culture medium are all through 121 DEG C, 1.1kg/cm2's
Autoclave sterilization 20min.
Embodiment 1:
1, test material
For trying the pot variety No.05 that red palm material provides for Wuhan Gu Yifeng science service company limited.Test is used
Chemical reagent is purchased from Xin Youyuan bio tech ltd, Wuhan.
2, culture medium preparation
The preparation of minimal medium MS see<<plant tissue culture>>(Li Mingjun compile. Beijing: Chinese agriculture publishing house,
1992)。
All culture medium all add agar powder 7g/L, sucrose 30g/L and the plant growth regulator of variable concentrations, use
The pH value of the culture medium prepared is adjusted to 5.8 by NaOH and HCl of 0.1mol/L, and culture medium is through 121 DEG C, 1.1kg/cm2High temperature
Autoclaving 20min.
3, method
(1) on superclean bench, the callus of adventitious bud will be differentiated from culture medium with the tweezers through high-temperature sterilization
Upper taking-up, is placed in the inoculation dish of prior sterilized process, and is removed gently by remaining medium with aseptic operation cutter.Again will be long
Degree is more than or equal to 0.5cm but is less than adventitious bud stripping (Fig. 1 a and Fig. 1 b) gently from callus of 2cm, and is inoculated into elongation
Cultivate in culture medium, the every 2-3 of adventitious bud of other health one group, after band callus cuts, proceed enrichment culture.Stretch
Long culture medium is MS minimal medium and the 6-benzyladenine of additional 1.5mg/L, the 2,4 dichloro benzene oxygen second of 0.2mg/L
Acid, the agar powder of 7g/L and the sucrose of 30g/L；Proliferated culture medium is the 6-benzyl of MS minimal medium and additional 2.0mg/L
Adenine, the 2,4 dichlorophenoxyacetic acid of 0.2mg/L, the agar powder of 7g/L and the sucrose of 30g/L.Condition of culture is: temperature 25
DEG C, intensity of illumination 1500Lux, illumination every day 16h, cultivates through 40～60d, it is thus achieved that healthy red palm tissue cultured seedling.
(2) on superclean bench, will cultivate, through 40～60d, the red palm tissue cultured seedling obtained with the tweezers through high-temperature sterilization
Take out from culture medium, be placed in the inoculation dish of prior sterilized process, and with aseptic operation cutter, remaining medium gone gently
Remove.Choose plant height 2cm and above and possess the health red palm tissue cultured seedling of 2 joints and above stipes, with scalpel at it from bottom to top
Section 1 top 2mm at crosscutting, form upper and lower two parts (Fig. 2 a and Fig. 2 b), upper part is inoculated in root media, bottom
Divide and be inoculated in elongation medium, continue to cultivate.If having the adventitious bud of curtailment 0.5cm or length to be more than or equal to 0.5cm but little
In the adventitious bud of 2cm, its inoculation method is with the related operating method of embodiment 1 (1).Root media be MS minimal medium and
The additional indolebutyric acid of 0.8mg/L, the activated carbon of 2.5g/L, the agar powder of 7g/L and the sucrose of 30g/L.Condition of culture is with real
Execute the related content of example 1 (1), red palm tissue cultured seedling (Fig. 3 b) of growth neat and consistent can be obtained.
Embodiment 2:
1, test material
For trying the pot variety No.47 that red palm material provides for Wuhan Gu Yifeng science service company limited.Test is used
Chemical reagent is purchased from Xin Youyuan bio tech ltd, Wuhan.
2, culture medium preparation
With in embodiment 12, culture medium preparation.
3, method
This method is applicable to red palm tissue cultured seedling and grows uneven situation.
On superclean bench, with the tweezers through high-temperature sterilization, the callus of band adventitious bud is taken out from culture medium,
It is placed in the inoculation dish of prior sterilized process, and with aseptic operation cutter, remaining medium is removed gently.Again length is less than
The every 2-3 of adventitious bud of 0.5cm one group, after band callus cuts, proceed enrichment culture；Length is more than or equal to 0.5cm
But the adventitious bud less than 2cm peels off (Fig. 1) from callus gently, is inoculated in elongation medium cultivation；Plant height 2cm and with
Go up, possess the health red palm tissue cultured seedling of 2 joints and above stipes, with scalpel horizontal stroke at its Section 1 top about 2mm from bottom to top
Cutting, form upper and lower two parts (Fig. 2 a and Fig. 2 b), upper part is inoculated in root media, and lower part is inoculated in elongation medium,
Continue to cultivate about 40d.
Proliferated culture medium is MS minimal medium and the 6-benzyladenine of additional 1.8mg/L, the 2,4-of 0.15mg/L
Dichlorphenoxyacetic acid, the agar powder of 7g/L and the sucrose of 30g/L；Elongation medium is MS minimal medium and additional 1.0mg/
The 6-benzyladenine of L, the 2,4 dichlorophenoxyacetic acid of 0.1mg/L, the agar powder of 7g/L and the sucrose of 30g/L；Root culture
Base is MS minimal medium and the indolebutyric acid of additional 0.5mg/L, the activated carbon of 2.5g/L, the agar powder of 7g/L and 30g/L
Sucrose.Condition of culture is temperature 25 DEG C, intensity of illumination 2000Lux, and illumination every day 16h can obtain the most neat health red
Palm tissue cultured seedling.
The content that this specification is not described in detail belongs to prior art known to professional and technical personnel in the field.
Claims (9)
1. a red palm tissue-culturing rapid propagation inoculation method, it is characterised in that it comprises the steps:
Step 1: the cultivation of red palm tissue cultured seedling；
The adventitious bud that anthurium andraeanum callus differentiation obtains is peeled off from callus, for the length health less than 0.5cm not
Every 2～3 one group of normal bud, after band callus cuts, is inoculated on proliferated culture medium cultivation, is more than or equal to for length
0.5cm but healthy adventitious bud less than 2cm is inoculated in elongation medium cultivation, for the length health more than or equal to 2cm not
Normal bud is inoculated on root media cultivation, and enrichment culture, elongation are cultivated and the condition of culture of root culture is: temperature 24～
26 DEG C, intensity of illumination 1500～2500Lux, illumination every day 15～17h, cultivate through 40～60d, it is thus achieved that healthy red palm group is trained
Seedling；
Step 2: the red numerous inoculation of palm tissue culture sprout quick；
From the health red palm tissue cultured seedling that step 1 obtains, choose plant height 2cm and more than, and possess 2 joints and the health of above stipes
Red palm tissue cultured seedling, in inoculation dish, on Seedling stem stipes Section 1 top 1.8 from bottom to top of the health chosen red palm tissue cultured seedling
～crosscutting at 2.2mm, form upper and lower two parts, upper part is inoculated in root media, and lower part is inoculated in elongation medium
Continuing to cultivate, condition of culture is identical with step 1, can obtain the red palm tissue cultured seedling that growth is neat.
Red palm tissue-culturing rapid propagation inoculation method the most according to claim 1, it is characterised in that: described elongation medium is MS base
Basal culture medium and the material of additional different components, wherein accessory ingredients include 1.0～1.5mg/L 6-benzyladenine, 0.05
～the 2 of 0.2mg/L, the agar powder of the sucrose of 4-dichlorphenoxyacetic acid, 28～32g/L, 6～8g/L, described elongation medium
PH value is 5.8.
Red palm tissue-culturing rapid propagation inoculation method the most according to claim 1, it is characterised in that: described root media is MS base
Basal culture medium and the material of additional different components, wherein accessory ingredients includes indolebutyric acid, 2～the 3g/L of 0.5～1.0mg/L
The agar powder of the sucrose of activated carbon, 28～32g/L, 6～8g/L, the pH value of described root media is 5.8.
Red palm tissue-culturing rapid propagation inoculation method the most according to claim 1, it is characterised in that: described proliferated culture medium is MS base
Basal culture medium and the material of additional different components, wherein accessory ingredients include 1.0～3.0mg/L 6-benzyladenine, 0.1～
The 2 of 0.5mg/L, the agar powder of the sucrose of 4-dichlorphenoxyacetic acid, 28～32g/L, 6～8g/L, the PH of described proliferated culture medium
Value is 5.8.
Red palm tissue-culturing rapid propagation inoculation method the most according to claim 2, it is characterised in that: the pH value of described elongation medium
Regulated by NaOH and HCl.
Red palm tissue-culturing rapid propagation inoculation method the most according to claim 3, it is characterised in that: the pH value of described root media
Regulated by NaOH and HCl.
Red palm tissue-culturing rapid propagation inoculation method the most according to claim 4, it is characterised in that: the pH value of described proliferated culture medium
Regulated by NaOH and HCl.
8. according to the red palm tissue-culturing rapid propagation inoculation method described in claim 2 or 4, it is characterised in that: described 6-benzyladenine
With 2, the NaOH of 4-dichlorphenoxyacetic acid 0.1mol/L or 95% ethanol dissolve, then are configured to respectively after aquae destillata dilution
The mother solution of 0.5mg/ml, loads in volumetric flask, is placed in 3～5 DEG C of Refrigerator stores.
Red palm tissue-culturing rapid propagation inoculation method the most according to claim 3, it is characterised in that: described indolebutyric acid is used
The NaOH of 0.1mol/L or 95% ethanol dissolve, then with being configured to the mother solution of 0.5mg/ml after aquae destillata dilution, load volumetric flask
In, it is placed in 3～5 DEG C of Refrigerator stores.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108377910A (en) * | 2018-04-25 | 2018-08-10 | 武汉市农业科学院 | A kind of tissue culture method using red palm vein induction differentiation adventitious bud |
| CN112522301A (en) * | 2020-12-11 | 2021-03-19 | 山东和正生态农业开发有限公司 | Method for improving conversion efficiency of anthurium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904595A (en) * | 2015-05-19 | 2015-09-16 | 锡山区先锋家庭农场 | Anthurium adndraeanum rapid propagation method |
| CN105010141A (en) * | 2015-07-09 | 2015-11-04 | 虞龙 | Anthurium rapid propagation method |
-
2016
- 2016-08-04 CN CN201610633137.XA patent/CN106165647B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904595A (en) * | 2015-05-19 | 2015-09-16 | 锡山区先锋家庭农场 | Anthurium adndraeanum rapid propagation method |
| CN105010141A (en) * | 2015-07-09 | 2015-11-04 | 虞龙 | Anthurium rapid propagation method |
Non-Patent Citations (2)
| Title |
|---|
| AISU GU ET.AL.,: "Regeneration of Anthurium andraeanum from Leaf Explants and Evaluation of Microcutting Rooting and Growth under Different Light Qualities", 《HORTSCIENCE》 * |
| 汪希强等: "红掌组培快繁技术优化研究", 《安徽农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108377910A (en) * | 2018-04-25 | 2018-08-10 | 武汉市农业科学院 | A kind of tissue culture method using red palm vein induction differentiation adventitious bud |
| CN108377910B (en) * | 2018-04-25 | 2020-01-10 | 武汉市农业科学院 | Tissue culture method for inducing and differentiating adventitious buds by using anthurium andraeanum veins |
| CN112522301A (en) * | 2020-12-11 | 2021-03-19 | 山东和正生态农业开发有限公司 | Method for improving conversion efficiency of anthurium |
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