CN106153791A - The method that beta cyclodextrin extracts compound recipe XUEZHINING prescription is optimized based on fingerprint pattern technology - Google Patents
The method that beta cyclodextrin extracts compound recipe XUEZHINING prescription is optimized based on fingerprint pattern technology Download PDFInfo
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8686—Fingerprinting, e.g. without prior knowledge of the sample components
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Abstract
The invention discloses and optimize, based on fingerprint pattern technology, the method that beta cyclodextrin extracts compound recipe XUEZHINING prescription, comprise the steps: step one, choose many group recipe quantity compound recipe XUEZHINING, prepare XUEZHINING water extract and XUEZHINING beta cyclodextrin extract respectively；Step 2, set up described XUEZHINING water extract and the finger printing of described XUEZHINING beta cyclodextrin extract respectively, choose multiple total fingerprint peaks；Step 3, described total peak is carried out medical material ownership, by the chromatographic peak area in described total fingerprint peaks, calculate corresponding chromatographic peak peak area contentStep 4, according to the peak area content of total fingerprint peaks, the chromatographic peak similarity in different medical materials after calculation optimization respectively in the chromatographic peak area of fingerprint peaks total in described XUEZHINING water extract and described XUEZHINING beta cyclodextrin extractMinima, so that it is determined that each medical material consumption in compound recipe XUEZHINING prescription after final optimization pass.
Description
Technical field
The present invention relates to extracting technique of Chinese medicine, be specifically related to optimize beta-schardinger dextrin-based on fingerprint pattern technology and extract compound recipe blood
The method of the peaceful prescription of fat.
Background technology
Chinese medicine compound XUEZHINING is recorded in the version Pharmacopoeia of the People's Republic of China one in 2010, by Semen Cassiae, system head
Crow, Folium Nelumbinis, Fructus Crataegi four taste Chinese medicine composition, have blood-activating and qi-promoting, effect of blood stasis dispelling blood fat reducing, it is adaptable to the stagnant caused hyperlipemia of the blood vessels stasis of blood
Disease.Cyclodextrin (Cyclodextrin is called for short CD) and derivant thereof are development in recent years novel medicinal materials faster, have life
Thing toxicity is low, the feature of good water solubility.The α that beta-schardinger dextrin-(β-CD) is starch to be obtained after glucosyltransferase ferments-
The cyclic oligomer that Isosorbide-5-Nitrae-glucoside is bonded, it comprises 7 glucopyranose units.There is interior hydrophilic spy hydrophobic, outer
Different molecular structure, can form inclusion with multiple " object " medicine, such that it is able to increase the dissolubility of medicine, stability and improve
Bioavailability.Domestic scholars utilizes cyclodextrin aqueous solution to extract the medical materials such as Flos Lonicerae, Semen Ginkgo, Fructus Choerospondiatis, Rhizoma Polygoni Cuspidati, finds
Cyclodextrin is to flavonoid, and the composition such as Anthraquinones has the effect increasing extraction ratio, improving dissolution rate, and prompting cyclodextrin is to medicine
Molecule has selection clathration, can improve the extraction ratio of active ingredient of Chinese herbs.
Chinese medicine fingerprint technology spectrum is a kind of comprehensive, quantifiable identification of means, by modern information technologies and matter
Component analysis means can reflect kind and the quantity of contained chemical composition in Chinese medicine and preparation thereof the most all sidedly, and then to medicine matter
Amount carries out whole description and evaluation.Compared with tradition extraction process by water, it is effective that beta-schardinger dextrin-extraction XUEZHINING new method can increase it
The extraction ratio of composition, and then increase the utilization rate of medical material, but owing to beta-schardinger dextrin-has molecule selectivity, to composition in XUEZHINING
There is different extraction effect, and the ratio of Chinese medicine ingredients, stable content are to ensure that the premise of its curative effect, for ensureing that beta-schardinger dextrin-carries
Take XUEZHINING new method drug quality and consistent with traditional extraction process extract component ratio, content, it is ensured that drug effect and medication peace
Entirely, need further to be optimized based on the prescription of compound recipe XUEZHINING under beta-schardinger dextrin-extraction process.
Summary of the invention
The present invention has designed and developed and has optimized, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription,
The goal of the invention of the present invention is to solve by using beta-schardinger dextrin-to extract compound recipe XUEZHINING medical material, it is ensured that extract component ratio, contain
Reduce prescription Chinese crude drug consumption in the case of amount is consistent, draw the problem of prescription consumption after optimization.
The technical scheme that the present invention provides is:
Optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription, comprise the steps:
Step one, choose many group recipe quantity compound recipe XUEZHINING, prepare XUEZHINING water extract and XUEZHINING β-ring respectively
Dextrin extract；
Step 2, set up described XUEZHINING water extract and the fingerprint image of described XUEZHINING beta-schardinger dextrin-extract respectively
Spectrum, chooses multiple total fingerprint peaks；
Step 3, described total peak is carried out medical material ownership, by the chromatographic peak area in described total fingerprint peaks, calculate
Corresponding chromatographic peak peak area contentIn formula, Xi is total fingerprint described in beta-schardinger dextrin-extract under conventional prescriptions
A certain composition chromatographic peak area in peak, Mi is the medicine quality of this synergy medical material under conventional prescriptions；
Step 4, according to the chromatographic peak area of fingerprint peaks total in described XUEZHINING water extract and described XUEZHINING β-
The peak area content of total fingerprint peaks, computer chromatography peak similarity in cyclodextrin extractMinima,
So that it is determined that each medical material consumption in compound recipe XUEZHINING prescription after final optimization pass；In formula, xi=mi × si, xi are under prescription after optimizing
A certain composition chromatographic peak area in all total fingerprint peakses of beta-schardinger dextrin-extract, mi is this synergy medical material after optimization
Medicine quality, si is the peak area content of this chromatographic peak, and yi is to extract with beta-schardinger dextrin-in total fingerprint peaks described in water extract
Having the chromatographic peak area that a certain composition chromatographic peak in fingerprint peaks is corresponding described in thing, n is the quantity at described total peak,For
Similarity degree.
Preferably, in described step one, described recipe quantity compound recipe XUEZHINING according to weight fraction by following components group
Become: Semen Cassiae 3 parts, Radix Polygoni Multiflori Preparata 2 parts, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part.
Preferably, in described step one, described XUEZHINING water extract preparation technology includes: according to recipe quantity compound recipe
XUEZHINING weighs Semen Cassiae 3g, Radix Polygoni Multiflori Preparata 2g, Folium Nelumbinis 1.5g, Fructus Crataegi 1g respectively, adds 25 times amount water, soaks 1 hour, extracts 3
Secondary, each 2 hours, extracting solution merged after filtering, and obtains described XUEZHINING water extract.
Preferably, in described step one, described XUEZHINING beta-schardinger dextrin-extract preparation technology includes: according to prescription
Amount compound recipe XUEZHINING weighs Semen Cassiae 3g, Radix Polygoni Multiflori Preparata 2g, Folium Nelumbinis 1.5g, Fructus Crataegi 1g respectively, adds and accounts for medical material amount 5% β-ring paste
Essence, 25 times amount water, soak 1 hour, extract 3 times, each 2 hours, extracting solution merged after filtering, and obtains described XUEZHINING β-ring and sticks with paste
Essence extract.
Preferably, in described step 2, choose 34 total fingerprint peakses.
Preferably, in described step 3, described total peak is carried out medical material ownership and comprises the steps:
Step a, weigh recipe quantity feminine gender Semen Cassiae compound recipe, negative Radix Polygoni Multiflori Preparata compound recipe, negative Folium Nelumbinis compound recipe, negative mountain respectively
Short, bristly hair or beard compound recipe, and prepare water extract and beta-schardinger dextrin-extract respectively；
Step b, setting up described water extract and the finger printing of described beta-schardinger dextrin-extract respectively, it is with 34 altogether
Fingerprint peaks is had to compare；
Step c, determining that 27 fingerprint peakses belong to Semen Cassiae, 4 fingerprint peakses belong to Radix Polygoni Multiflori Preparata, 4 fingerprint peakses ownership
In Folium Nelumbinis, 1 fingerprint peaks belongs to Fructus Crataegi.
Preferably, described step a also includes negative Semen Cassiae and Radix Polygoni Multiflori Preparata compound recipe, at negative Radix Polygoni Multiflori Preparata and Fructus Crataegi
Side.
Preferably, in described step a, described negative Semen Cassiae compound recipe is composed of the following components according to weight fraction:
Radix Polygoni Multiflori Preparata 2 parts, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part；Described negative Radix Polygoni Multiflori Preparata compound recipe is composed of the following components according to weight fraction: Semen Cassiae
Son 3 parts, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part；Described negative Folium Nelumbinis compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3 parts,
Radix Polygoni Multiflori Preparata 2 parts, Fructus Crataegi 1 part；Described negative Fructus Crataegi compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3 parts, Radix Polygoni Multiflori Preparata
2 parts, 1.5 parts of Folium Nelumbinis；Described negative Semen Cassiae and Radix Polygoni Multiflori Preparata compound recipe are composed of the following components according to weight fraction: 1.5 parts of Folium Nelumbinis,
Fructus Crataegi 1 part；Described negative Radix Polygoni Multiflori Preparata and Fructus Crataegi prescription are composed of the following components according to weight fraction: Semen Cassiae 3 parts, Folium Nelumbinis 1.5
Part.
Preferably, in described step 4, calculate described chromatographic peak similarity R minima, multiple after determining final optimization pass
In side's XUEZHINING prescription, Semen Cassiae is by for the 90.7% of original prescription consumption, and Radix Polygoni Multiflori Preparata is by for the 80.7% of original prescription consumption, Folium Nelumbinis
By for the 80.1% of original prescription consumption, Fructus Crataegi consumption keeps constant.
The present invention is had the advantages that compared with prior art
1, beta-schardinger dextrin-extracts in sample 34 total fingerprint peakses (except No. 21 peaks) peak area has and increases in various degree, enters one
Step demonstrates beta-schardinger dextrin-and has molecule selectivity, owing to the two forms identical in extract component, but has difference on extracted amount
Different, therefore use improve after newly improve extent similarity, it has the big difference appearing suddenly in sample, reflects components group delicately
In the deviation big to set proportioning of some composition, in compound recipe optimizes, it is more favourable to the characteristic of components group proportioning undulated control
After ensureing to optimize, the chemical composition ratio of compound recipe and tradition extract the concordance of sample, and then ensure the consistent of drug effect；
2, this experiment uses quantitative finger print atlas assistant analysis means first, and XUEZHINING compound recipe is optimized research, warp
Cross optimization similarity and brought up to 0.924 by 0.837, basically identical with calculated value 0.925, it was demonstrated that the feasibility of method, also
Providing a new way for solving Similar Problems, after optimizing, in compound recipe, Semen Cassiae, Radix Polygoni Multiflori Preparata, Folium Nelumbinis are reduced to former multiple respectively simultaneously
The 90.7%, 80.7% and 80.1% of side's consumption, the minimizing of medical material consumption is to Chinese medicine protection of resources and reduces industrialized production
Cost is significant.
Accompanying drawing explanation
Fig. 1 is compound recipe XUEZHINING water extract high-efficient liquid phase chromatogram of the present invention.
Fig. 2 is compound recipe XUEZHINING beta-schardinger dextrin-extract high-efficient liquid phase chromatogram of the present invention.
Fig. 3 is stilbene reference substance high-efficient liquid phase chromatogram.
Fig. 4 is XUEZHINING tradition water extraction sample fingerprint image spectrogram.
Fig. 5 is that XUEZHINING beta-schardinger dextrin-extracts sample fingerprint image spectrogram.
Fig. 6 is that XUEZHINING has fingerprint peaks chromatogram.
Fig. 7 is negative Semen Cassiae compound recipe fingerprint image spectrogram.
Fig. 8 is negative Radix Polygoni Multiflori Preparata compound recipe fingerprint image spectrogram.
Fig. 9 is negative Folium Nelumbinis compound recipe fingerprint image spectrogram.
Figure 10 is negative Fructus Crataegi compound recipe fingerprint image spectrogram.
Figure 11 is negative Semen Cassiae and Radix Polygoni Multiflori Preparata compound recipe fingerprint image spectrogram.
Figure 12 is negative Radix Polygoni Multiflori Preparata and Fructus Crataegi compound recipe fingerprint image spectrogram.
Detailed description of the invention
The present invention is described in further detail below in conjunction with the accompanying drawings, to make those skilled in the art with reference to description literary composition
Word can be implemented according to this.
As shown in Fig. 1～12, the invention provides and optimize beta-schardinger dextrin-extraction compound recipe XUEZHINING based on fingerprint pattern technology
The method of prescription, comprises the steps:
Step one, choose many group recipe quantity compound recipe XUEZHINING, prepare XUEZHINING water extract and XUEZHINING β-ring respectively
Dextrin extract；
Step 2, set up described XUEZHINING water extract and the fingerprint image of described XUEZHINING beta-schardinger dextrin-extract respectively
Spectrum, chooses multiple total fingerprint peaks；
Step 3, described total peak is carried out medical material ownership, by the chromatographic peak area in described total fingerprint peaks, calculate
Corresponding chromatographic peak peak area contentIn formula, Xi is total fingerprint described in beta-schardinger dextrin-extract under conventional prescriptions
A certain composition chromatographic peak area in peak, Mi is the medicine quality of this synergy medical material under conventional prescriptions；
Step 4, according to the chromatographic peak area of fingerprint peaks total in described XUEZHINING water extract and described XUEZHINING β-
The peak area content of total fingerprint peaks, computer chromatography peak similarity in cyclodextrin extractMinima,
So that it is determined that each medical material consumption in compound recipe XUEZHINING prescription after final optimization pass；In formula, xi=mi × si, xi are under prescription after optimizing
A certain composition chromatographic peak area in all total fingerprint peakses of beta-schardinger dextrin-extract, mi is this synergy medical material after optimization
Medicine quality, si is the peak area content of this chromatographic peak, and yi is to extract with beta-schardinger dextrin-in total fingerprint peaks described in water extract
Having the chromatographic peak area that a certain composition chromatographic peak in fingerprint peaks is corresponding described in thing, n is the quantity at described total peak,For
Similarity degree.
In another kind of embodiment, in step one, recipe quantity compound recipe XUEZHINING according to weight fraction by following components group
Become: Semen Cassiae 3 parts, Radix Polygoni Multiflori Preparata 2 parts, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part.
In another kind of embodiment, in step one, XUEZHINING is water extract preparation technology include: according to recipe quantity compound recipe
XUEZHINING weighs Semen Cassiae 3g, Radix Polygoni Multiflori Preparata 2g, Folium Nelumbinis 1.5g, Fructus Crataegi 1g respectively, adds 25 times amount water, soaks 1 hour, extracts 3
Secondary, each 2 hours, extracting solution merged after filtering, and obtains described XUEZHINING water extract.
In another kind of embodiment, in step one, XUEZHINING is beta-schardinger dextrin-extract preparation technology include: according to prescription
Amount compound recipe XUEZHINING weighs Semen Cassiae 3g, Radix Polygoni Multiflori Preparata 2g, Folium Nelumbinis 1.5g, Fructus Crataegi 1g respectively, adds and accounts for medical material amount 5% β-ring paste
Essence, 25 times amount water, soak 1 hour, extract 3 times, each 2 hours.Extracting solution merges after filtering, and obtains described XUEZHINING β-ring and sticks with paste
Essence extract.
In another kind of embodiment, in step 3, described total peak is carried out medical material ownership and comprises the steps:
Step a, weigh recipe quantity feminine gender Semen Cassiae compound recipe, negative Radix Polygoni Multiflori Preparata compound recipe, negative Folium Nelumbinis compound recipe, negative mountain respectively
Short, bristly hair or beard compound recipe, and prepare water extract and beta-schardinger dextrin-extract respectively；
Step b, setting up described water extract and the finger printing of described beta-schardinger dextrin-extract respectively, it is with 34 altogether
Fingerprint peaks is had to compare；
Step c, determining that 27 fingerprint peakses belong to Semen Cassiae, 4 fingerprint peakses belong to Radix Polygoni Multiflori Preparata, 4 fingerprint peakses ownership
In Folium Nelumbinis, 1 fingerprint peaks belongs to Fructus Crataegi.
In another kind of embodiment, step a also includes negative Semen Cassiae and Radix Polygoni Multiflori Preparata compound recipe, negative Radix Polygoni Multiflori Preparata and Fructus Crataegi
Prescription.
In another kind of embodiment, in step a, negative Semen Cassiae compound recipe is composed of the following components according to weight fraction:
Radix Polygoni Multiflori Preparata 2 parts, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part；Negative Radix Polygoni Multiflori Preparata compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3
Part, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part；Negative Folium Nelumbinis compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3 parts, Radix Polygoni Multiflori Preparata 2
Part, Fructus Crataegi 1 part；Negative Fructus Crataegi compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3 parts, Radix Polygoni Multiflori Preparata 2 parts, Folium Nelumbinis 1.5
Part；Negative Semen Cassiae and Radix Polygoni Multiflori Preparata compound recipe are composed of the following components according to weight fraction: 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part；Negative system
The Radix Polygoni Multiflori and Fructus Crataegi prescription are composed of the following components according to weight fraction: Semen Cassiae 3 parts, 1.5 parts of Folium Nelumbinis.
In another kind of embodiment, in step 4, calculate described chromatographic peak similarity R minima, determine final optimization pass
In rear compound recipe XUEZHINING prescription, Semen Cassiae is by for the 90.7% of original prescription consumption, Radix Polygoni Multiflori Preparata by for the 80.7% of original prescription consumption,
Folium Nelumbinis is by for the 80.1% of original prescription consumption, and Fructus Crataegi consumption keeps constant.
Embodiment
1 material
1.1 medicine
Semen Cassiae (place of production: Vietnam), Radix Polygoni Multiflori Preparata (place of production: Sichuan), Folium Nelumbinis (place of production: Shandong), Fructus Crataegi (place of production: Chengde).
1.2 reagent
1.3 instrument
2 methods and result
2.1 compound recipe XUEZHINING Chinese medicine fingerprints
2.1.1 chromatographic condition
Chromatographic column: Acquity UPLC BEH shield RP18 (2.1 × 100mm, 1.7 μm)；Flowing phase: methanol (A)-
0.1% aqueous formic acid (B), gradient elution program see table；Flow velocity: 0.2mL/min；Column temperature: 45 DEG C；Detection wavelength:
280nm；Analysis time: 40min；Sample size 3 μ L；As shown in Figures 1 to 3, for XUEZHINING high-efficient liquid phase chromatogram.
Table 1 gradient elution table
2.1.2 prepared by sample solution
XUEZHINING tradition extraction process by water
Medical material (Semen Cassiae 3g is weighed according to XUEZHINING compound recipe；Radix Polygoni Multiflori Preparata 2g；Folium Nelumbinis 1.5g；Fructus Crataegi 1g), add 25 times amount
Water, soaks 1 hour, extracts 3 times, each 2 hours.Extracting solution merges after filtering, and is XUEZHINING tradition water and gets sample product.
XUEZHINING beta-schardinger dextrin-extraction process
Medical material (Semen Cassiae 3g is weighed according to XUEZHINING compound recipe；Radix Polygoni Multiflori Preparata 2g；Folium Nelumbinis 1.5g；Fructus Crataegi 1g), add and account for medical material
Measure 5% beta-schardinger dextrin-, 25 times amount water, soak 1 hour, extract 3 times, each 2 hours.Extracting solution merges after filtering, and is blood fat
Peaceful beta-schardinger dextrin-extracts sample.
Need testing solution is prepared precision and is measured 1mL extraction sample, puts in 10mL brown volumetric flask, adds methanol constant volume to carving
Degree, shakes up, and ultrasonic 30min, 10000r min-1 is centrifuged 10min, and supernatant, through 0.22 μm membrane filtration, collects subsequent filtrate i.e.
For need testing solution.
Prepared by reference substance solution
Take stilbene glucoside reference substance appropriate, accurately weighed, put in 10mL brown volumetric flask, add methanol constant volume to scale,
Shake up.
2.1.3 XUEZHINING finger printing
Weighing medical material 10 parts by XUEZHINING compound recipe, respectively by legal system available test sample solution below " 2.1.2 " item, sample introduction divides
Analysis, 10 batches of traditional water extraction samples extract sample finger printing (shown in Fig. 4, Fig. 5) with beta-schardinger dextrin-.Contrast XUEZHINING tradition carries
Taking and extract knowable to sample fingerprint chromatogram with β-CD, two kinds of extracting method sample fingerprint peaks numbers are identical, according to respective 10 batch samples
Demarcate 34 total fingerprint peakses (as shown in Figure 6) altogether.
2.1.4 total fingerprint peaks ownership
Weigh negative Semen Cassiae, Radix Polygoni Multiflori Preparata, Folium Nelumbinis, Fructus Crataegi compound recipe by compound recipe amount respectively, prepare by sample under " 2.1.2 " item
Method prepares each medical material feminine gender medical material sample, sample introduction analysis, is belonged to 34 total fingerprint peakses, the results are shown in Table 2 and table
3, negative sample need testing solution fingerprint map analyzing result is shown in Fig. 7～12.
Table 2 has fingerprint peaks ownership
Note: in table, chromatographic peak title mass spectrometry premenstruum is determined.
Table 3 has fingerprint peaks each medical material ownership quantity
2.2 sample determination
Weigh medical material 6 parts according to XUEZHINING compound recipe respectively, press " 2.1.2 " item with tradition Aqueous extracts and beta-schardinger dextrin-extracting solution
Lower section legal system available test sample solution, sample introduction analysis, record 34 total peak areas and meansigma methods, result is as shown in table 4, table 5.
Table 4 XUEZHINING tradition water gets sample product measurement result
Table 5 XUEZHINING beta-schardinger dextrin-extracts sample determination result
2.3 similarity evaluation
Experiment uses and newly improves extent similarity (similarity Q ') as method for evaluating similarity after improving, and newly improves journey
Degree similarity can appear the big difference in sample suddenly, distinguishes the sample different from the distribution of the total variances same difference of sample for reference, spirit
Reflect the deviation that in components group, some composition is big to set proportioning, it is adaptable to the control to the fluctuation of components group proportioning quickly, calculate
Formula:In formula, Xi is to have in fingerprint peaks described in beta-schardinger dextrin-extract under conventional prescriptions
A certain composition chromatographic peak area, yi be in total fingerprint peaks described in water extract with described in beta-schardinger dextrin-extract total fingerprint peaks
In the corresponding chromatographic peak area of a certain composition chromatographic peak, n is the quantity at described total peak,For similarity degree.
Use newly to improve extent similarity and calculate XUEZHINING tradition water and carry and with beta-schardinger dextrin-extraction group similarity result be
0.837, show that under conventional prescriptions, beta-schardinger dextrin-extraction XUEZHINING there are differences with tradition extraction sample, therefore needs to consider blood fat
Peaceful compound recipe is optimized, to can be similar to tradition extraction group to greatest extent, it is ensured that drug effect is consistent.Result is as shown in table 6.
Table 6 XUEZHINING cyclodextrin group puies forward group similarity with tradition water
2.4 XUEZHINING compound recipes optimize
Beta-schardinger dextrin-is extracted XUEZHINING compound recipe optimization is based on every kind of composition high-efficient liquid phase chromatogram peak in finger printing
The linear additivity of area fraction, as shown by the equation: xi=mi × si, in formula, xi extracts for beta-schardinger dextrin-under prescription after optimizing
A certain composition chromatographic peak area in all total fingerprint peakses of thing, mi represents the medicine quality of this synergy medical material after optimization,
Si is the peak area content of this chromatographic peak.
Wherein, the peak area content formula of this chromatographic peak isIn formula, Xi is that under conventional prescriptions, beta-schardinger dextrin-extracts
A certain composition chromatographic peak area in total fingerprint peaks described in thing, Mi is the medication matter of this synergy medical material under conventional prescriptions
Amount；With tradition water carry group the corresponding every kind of Component peak area of XUEZHINING finger printing as standard, keep face, beta-schardinger dextrin-extract peak
Long-pending content is constant, and new improvement extent similarity is evaluation index, ingredients consumption in calculation optimization XUEZHINING compound recipe, thus really
Determine beta-schardinger dextrin-extract XUEZHINING optimization compound recipe, wherein,In formula, yi is described in water extract
The chromatographic peak face that in total fingerprint peaks, a certain composition chromatographic peak in fingerprint peaks total with described in beta-schardinger dextrin-extract is corresponding
Long-pending.
2.4.1 peak area content
Beta-schardinger dextrin-extracts the peak area assay of 34 total fingerprint peakses in XUEZHINING compound recipe and the results are shown in Table shown in 7.
Table 7 beta-schardinger dextrin-extracts compound recipe XUEZHINING peak area cubage result
2.4.2 compound recipe is optimized
Beta-schardinger dextrin-extracts the compound recipe optimization of XUEZHINING, is using new improvement similarity Q as evaluation index, asks for similarity
Q maximum, therefore can be reduced to seek chromatographic peak similarity R minima,According to pre-stage test result,
Beta-schardinger dextrin-extraction compound recipe XUEZHINING relatively conventional prescriptions extraction 34 Component peak area of sample have to be increased, in various degree for making β-ring
It is consistent with tradition extraction sample composition ratio that dextrin extracts compound recipe XUEZHINING, it is desirable to reduce corresponding medical material usage amount, in this enforcement
In example, use LINGO9.0 software founding mathematical models, solve chromatographic peak similarity R and obtain minima (newly improvement similarity Q
Maximum) time corresponding ingredients decrement, result of calculation be Semen Cassiae, Radix Polygoni Multiflori Preparata, Folium Nelumbinis medical material decrement be respectively
0.2801g, 0.3859g, 0.2988g, fructus crataegi medicinal material amount remains unchanged, and this stylish improvement similarity Q is 0.925, it is thus determined that
In the optimization compound recipe that beta-schardinger dextrin-extracts, Semen Cassiae, Radix Polygoni Multiflori Preparata, Folium Nelumbinis reduce to the 90.7% of former compound recipe consumption, 80.7% He respectively
80.1%, Fructus Crataegi consumption is constant.
2.5 optimize compound recipe checking
Weigh 3 parts of compound recipe XUEZHINING according to optimization formulation, prepare by XUEZHINING beta-schardinger dextrin-extraction process under " 2.1.2 " item
Sample, analyzes the peak area (as shown in table 8) measuring and recording total fingerprint peaks, according to new improvement similarity Q, passes with XUEZHINING
The system water product measurement result that gets sample is standard, calculates beta-schardinger dextrin-and extracts and optimize the similarity result that XUEZHINING compound recipe extracts with tradition
(as shown in table 9), calculating average degree similarity is 0.924, basically identical with calculated value.
Table 8 beta-schardinger dextrin-extracts and optimizes XUEZHINING compound recipe result
Table 9 Similarity Measure result
Shown in table 8,9, it is optimized by compound recipe XUEZHINING prescription will be extracted based on beta-schardinger dextrin-, is reducing medical material
Ensure that the equivalence of prescription while consumption, the minimizing of medical material consumption is to Chinese medicine protection of resources and reduces industrialized production one-tenth
This is significant.
Although embodiment of the present invention are disclosed as above, but it is not restricted in description and embodiment listed
Using, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easily
Realizing other amendment, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details with shown here as the legend with description.
Claims (9)
1. based on fingerprint pattern technology optimize beta-schardinger dextrin-extract compound recipe XUEZHINING prescription method, it is characterised in that include as
Lower step:
Step one, choose many group recipe quantity compound recipe XUEZHINING, prepare XUEZHINING water extract and XUEZHINING beta-schardinger dextrin-respectively
Extract；
Step 2, set up described XUEZHINING water extract and the finger printing of described XUEZHINING beta-schardinger dextrin-extract respectively,
Choose multiple total fingerprint peaks；
Step 3, described total peak is carried out medical material ownership, by the chromatographic peak area in described total fingerprint peaks, calculate relatively
The chromatographic peak peak area content answeredIn formula, Xi is to have in fingerprint peaks described in beta-schardinger dextrin-extract under conventional prescriptions
A certain composition chromatographic peak area, Mi is the medicine quality of this synergy medical material under conventional prescriptions；
Step 4, chromatographic peak area and described XUEZHINING β-ring according to fingerprint peaks total in described XUEZHINING water extract are stuck with paste
The peak area content of total fingerprint peaks, computer chromatography peak similarity in essence extractMinima, thus
Determine after final optimization pass each medical material consumption in compound recipe XUEZHINING prescription；In formula, xi=mi × si, xi are β-ring under prescription after optimizing
A certain composition chromatographic peak area in all total fingerprint peakses of dextrin extract, mi be optimize after the medication of this synergy medical material
Quality, si is the peak area content of this chromatographic peak, yi be in total fingerprint peaks described in water extract with beta-schardinger dextrin-extract institute
Stating the chromatographic peak area that a certain composition chromatographic peak in total fingerprint peaks is corresponding, n is the quantity at described total peak,For similar
Degree.
2. optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription as claimed in claim 1,
It is characterized in that, in described step one, described recipe quantity compound recipe XUEZHINING is composed of the following components according to weight fraction: Semen Cassiae
Son 3 parts, Radix Polygoni Multiflori Preparata 2 parts, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part.
3. optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription as claimed in claim 2,
It is characterized in that, in described step one, described XUEZHINING water extract preparation technology includes: according to recipe quantity compound recipe XUEZHINING
Weigh Semen Cassiae 3g, Radix Polygoni Multiflori Preparata 2g, Folium Nelumbinis 1.5g, Fructus Crataegi 1g respectively, add 25 times amount water, soak 1 hour, extract 3 times, every time
2 hours, extracting solution merged after filtering, and obtains described XUEZHINING water extract.
4. optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription as claimed in claim 3,
It is characterized in that, in described step one, described XUEZHINING beta-schardinger dextrin-extract preparation technology includes: according to recipe quantity compound recipe
XUEZHINING weighs Semen Cassiae 3g, Radix Polygoni Multiflori Preparata 2g, Folium Nelumbinis 1.5g, Fructus Crataegi 1g respectively, adds and accounts for medical material amount 5% beta-schardinger dextrin-, 25 times
Amount water, soaks 1 hour, extracts 3 times, and each 2 hours, extracting solution merged after filtering, and obtains described XUEZHINING beta-schardinger dextrin-and extracts
Thing.
5. optimizing at beta-schardinger dextrin-extraction compound recipe XUEZHINING based on fingerprint pattern technology as according to any one of claim 1-4
The method of side, it is characterised in that in described step 2, choose 34 total fingerprint peakses.
6. optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription as claimed in claim 5,
It is characterized in that, in described step 3, described total peak is carried out medical material ownership and comprises the steps:
Step a, to weigh recipe quantity feminine gender Semen Cassiae compound recipe, negative Radix Polygoni Multiflori Preparata compound recipe, negative Folium Nelumbinis compound recipe, negative Fructus Crataegi respectively multiple
Side, and prepare water extract and beta-schardinger dextrin-extract respectively；
Step b, setting up described water extract and the finger printing of described beta-schardinger dextrin-extract respectively, it is total with 34 refers to
Compare in stricture of vagina peak；
Step c, determining that 27 fingerprint peakses belong to Semen Cassiae, 4 fingerprint peakses belong to Radix Polygoni Multiflori Preparata, and 4 fingerprint peakses belong to lotus
Leaf, 1 fingerprint peaks belongs to Fructus Crataegi.
7. optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription as claimed in claim 6,
It is characterized in that, described step a also includes negative Semen Cassiae and Radix Polygoni Multiflori Preparata compound recipe, negative Radix Polygoni Multiflori Preparata and Fructus Crataegi prescription.
8. optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription as claimed in claim 7,
It is characterized in that, in described step a, described negative Semen Cassiae compound recipe is composed of the following components according to weight fraction: Radix Polygoni Multiflori Preparata 2
Part, 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part；Described negative Radix Polygoni Multiflori Preparata compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3 parts,
1.5 parts of Folium Nelumbinis, Fructus Crataegi 1 part；Described negative Folium Nelumbinis compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3 parts, Radix Polygoni Multiflori Preparata
2 parts, Fructus Crataegi 1 part；Described negative Fructus Crataegi compound recipe is composed of the following components according to weight fraction: Semen Cassiae 3 parts, Radix Polygoni Multiflori Preparata 2 parts, lotus
1.5 parts of leaf；Described negative Semen Cassiae and Radix Polygoni Multiflori Preparata compound recipe are composed of the following components according to weight fraction: 1.5 parts of Folium Nelumbinis, Fructus Crataegi 1
Part；Described negative Radix Polygoni Multiflori Preparata and Fructus Crataegi prescription are composed of the following components according to weight fraction: Semen Cassiae 3 parts, 1.5 parts of Folium Nelumbinis.
9. optimize, based on fingerprint pattern technology, the method that beta-schardinger dextrin-extracts compound recipe XUEZHINING prescription as claimed in claim 8,
It is characterized in that, in described step 4, calculate described chromatographic peak similarity R minima, determine compound recipe blood fat after final optimization pass
In peaceful prescription, Semen Cassiae is by for the 90.7% of original prescription consumption, and Radix Polygoni Multiflori Preparata is by for the 80.7% of original prescription consumption, and Folium Nelumbinis will be for former
The 80.1% of prescription consumption, Fructus Crataegi consumption keeps constant.
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