CN106153591A - A kind of measure the method for bacterial density in water sample - Google Patents
A kind of measure the method for bacterial density in water sample Download PDFInfo
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- CN106153591A CN106153591A CN201610667993.7A CN201610667993A CN106153591A CN 106153591 A CN106153591 A CN 106153591A CN 201610667993 A CN201610667993 A CN 201610667993A CN 106153591 A CN106153591 A CN 106153591A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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Abstract
The invention provides and a kind of measure the method for bacterial density in water sample, it comprises the steps: to use filtering with microporous membrane water sample to be measured, nucleic acid staining agent is used to dye under the conditions of lucifuge to filtering complete microporous filter membrane, and measure the fluorescence intensity of the microporous filter membrane after dyeing, then calculate the bacterial number of water sample to be measured according to the equation of linear regression illustrating bacterial number and fluorescence intensity corresponding relation, bacterial density is equal to the business of bacterial number with the volume of water sample to be measured；Cultivate the bacterial density that can quickly obtain this water sample to be measured when the present invention is without carrying out long to the antibacterial in water sample to be measured, and can carry out multiple water samples to be measured measuring in batches simultaneously, be effectively shortened the detection time.
Description
Technical field
The invention belongs to bacterial density detection technique field, relate to a kind of measuring the method for bacterial density in water sample.
Background technology
Bacterial density in the water bodys such as such as river, river, lake water, water for industrial use, Drinking Water and sea water is to evaluate
The important indicator of its clean level.The conventional method measuring the bacterial density in above-mentioned water body at present is first to gather water sample, then
Classifted training in the lab, is measured after waiting to turn out macroscopic bacterium colony again.But, cultivate macroscopic
Bacterium colony at least needs 1 15 day time (depending on the kind of antibacterial), the longest and complex operation；It addition, in the mistake cultivated
In journey, easy bacteria infection, not only affects measurement result, the technical merit of operator is required height, and this assay method is also
It is limited to the culture environment of laboratory；Furthermore, it is possible to the antibacterial carrying out artificial culture only accounts for few of bacterial species sum
Point, therefore, above-mentioned method is higher to the requirement of bacterial species, therefore needs to set up a set of simplicity, sensitive, quickly detects water sample
The method of middle bacterial density.
Summary of the invention
In order to solve the problems referred to above, the invention provides a kind of method of bacterial density in quick mensuration water sample.
For reaching above-mentioned purpose, the solution of the present invention is:
A kind of measuring the method for bacterial density in water sample, it comprises the steps:
Use filtering with microporous membrane water sample to be measured, use nucleic acid staining agent to filtering complete microporous filter membrane in lucifuge condition
Under dye, and measure the fluorescence intensity of the microporous filter membrane after dyeing, then according to illustrating bacterial number and fluorescence intensity
The equation of linear regression of corresponding relation calculates the bacterial number of water sample to be measured, and bacterial number with the business of the volume of water sample to be measured is
Bacterial density.
Wherein, the aperture of above-mentioned microporous filter membrane can be 0.22 μm.
Above-mentioned nucleic acid staining agent can be9green fluorescent nucleic acid stains
(Molecular Probes,Leiden,Netherlands)。
The assay method of above-mentioned fluorescence intensity is: with the micropore filter after the excitation light irradiation dyeing of 470nm at 25 DEG C
Film, then measures its fluorescence intensity at 510nm.
The construction method of above-mentioned equation of linear regression comprises the steps:
(1), obtain multiple have different totals number of bacteria containing bacterium solution；
(2), use multiple microporous filter membrane that each solution one_to_one corresponding Han bacterium is filtered；
(3) microporous filter membrane after, using nucleic acid staining agent to filter each dyes under the conditions of lucifuge；
(4) fluorescence intensity of the microporous filter membrane after blank microporous filter membrane and all of dyeing, is measured；
(5), the fluorescence intensity of microporous filter membrane and the relation of total number of bacteria are carried out curve fitting, obtain linear fit curve
And obtain the equation of linear regression of correspondence.
Wherein, the bacterial density containing bacterium solution can be 102、103、104、105、106、107Or 108Individual/mL, volume is permissible
It is 1 10mL.
Owing to using such scheme, the invention has the beneficial effects as follows:
The present invention first uses specific filtering with microporous membrane water sample to be measured, makes the antibacterial in this water sample to be measured all be enriched in
On microporous filter membrane, nucleic acid staining agent is then used to dye under the conditions of lucifuge to filtering complete microporous filter membrane and measure glimmering
Light intensity, calculates the antibacterial of water sample to be measured further according to the equation of linear regression of characterized bacterial number amount Yu fluorescence intensity corresponding relation
Quantity, the business finally according to bacterial number Yu the volume of water sample to be measured obtains bacterial density.
Owing to the antibacterial in water sample to be measured is all enriched on microporous filter membrane so that the bacterial number foot on this microporous filter membrane
To reach the Monitoring lower-cut of fluorescent quantitation detection, therefore, the present invention can be led to without the antibacterial in water sample to be measured is carried out cultivation
Cross fluorescent quantitation detection and quickly obtain the bacterial density of this water sample to be measured, there is higher sensitivity, and be not easily susceptible to sample
The impact of the fluorimetric factor of middle interference.
It addition, microporous filter membrane can be cut into same size, it is possible to the filter membrane after multi-disc being cut out by microplate reader
Carrying out fluorescent strength determining, therefore, the present invention can carry out batch simultaneously and measure multiple water samples to be measured, effectively shortens simultaneously
Detection time.
Detailed description of the invention
The invention provides and a kind of measure the method for bacterial density in water sample, it comprises the steps:
(1), obtain the water sample to be measured of certain volume, use this water sample to be measured of filtering with microporous membrane, obtain filtering complete
Microporous filter membrane, and discard filtrate；
(2) nucleic acid staining agent, is used to dye under the conditions of lucifuge to filtering complete microporous filter membrane, it is thus achieved that after dyeing
Microporous filter membrane；
(3) fluorescence intensity of the microporous filter membrane after dyeing, is measured；
(4), according to representing that the bacterial number equation of linear regression with the corresponding relation of fluorescence intensity calculates water body to be measured
Bacterial number；
(5) bacterial density of water sample to be measured, is calculated by below equation: the bacterial number of bacterial density=calculate/
The volume of water sample to be measured.
Wherein, in step (1), water sample to be measured can be river, lake water, water for industrial use, Drinking Water and sea water etc.,
Its volume can be 100 1000mL, this volume according to this water sample to be measured estimate bacteria containing amount or cleanliness factor is determined.
In step (1), the aperture of microporous filter membrane is 0.22 μm (PTFE membranes, Millipore, UK Ltd).
This microporous filter membrane can retain antibacterial, makes Enrichment of bacteria without the micropore through this microporous filter membrane on microporous filter membrane.
In step (1), microporous filter membrane should be in advance through sterilization treatment, in case the miscellaneous bacteria interference on microporous membrane surface is surveyed
Determine result.
In step (1), filtration can use gravity filtration, it would however also be possible to employ vacuum filtration.
In step (2), nucleic acid staining agent is9green fluorescent nucleic acid stains
(Molecular Probes,Leiden,Netherlands).This nucleic acid staining agent be a kind of can be with the core in bacterial cell
The fluorescent dye that acid combines.After nucleic acid staining agent nucleic acid in bacterial cell is combined, at the exciting light that wavelength is 470nm
Exciting down, this nucleic acid staining agent can launch fluorescence, and the intensity of the fluorescence launched just becomes with the quantity of the nucleic acid combined
Ratio, therefore, it can extrapolate the number of antibacterial by measuring fluorescence intensity.In order to ensure nucleic acid staining agent can with all carefully
The nucleic acid of bacterium combines, and the consumption of nucleic acid staining agent should be excessive.
In step (2), the time of dyeing is 5 ± 1min.
In step (2), need after dyeing to wash away unconjugated nucleic acid staining agent, thus prevent false positive.
In step (3), the assay method of fluorescence intensity is: with after the excitation light irradiation dyeing of 470nm at 25 DEG C
Microporous filter membrane, then measures its fluorescence intensity at 510nm.
In step (3), fluorescence intensity can be measured by microplate reader.At this time, it may be necessary to the micropore of a diameter of R is filtered
Film is cut into the circular filter membrane of a diameter of r=0.5cm.Because the circular filter membrane being only a diameter of r=0.5cm that microplate reader records
Fluorescence intensity Dr, so also needing to calculate fluorescence intensity D of the microporous filter membrane of a diameter of RR.Micropore in view of a diameter of R is filtered
Antibacterial on film is equally distributed, therefore, and the fluorescence intensity of the microporous filter membrane of a diameter of R
In step (4), the construction method of equation of linear regression comprises the steps:
(4 1), obtain multiple have different totals number of bacteria containing bacterium solution；
(4 2), use multiple microporous filter membrane that multiple solution one_to_one corresponding containing bacterium are filtered, obtain multiple filtration complete
Microporous filter membrane, and discard filtrate；
Each is filtered complete microporous filter membrane and dyes under the conditions of lucifuge by (4 3), use nucleic acid staining agent,
Microporous filter membrane after multiple dyeing；
(4 4), measure the fluorescence intensity of the microporous filter membrane after blank microporous filter membrane and all of dyeing；
(4 5), fluorescence with fluorescence intensity as abscissa, with total number of bacteria as vertical coordinate, to each microporous filter membrane above-mentioned
The corresponding relation of intensity and total number of bacteria carries out curve fitting, and obtains linear fit curve and obtains the linear regression side of correspondence
Journey.
Wherein, in step (4 1), in order to ensure the accuracy of equation of linear regression, the quantity containing bacterium solution the most more
Good.
In step (4 1), the total number of bacteria containing bacterium solution can be by the volume containing bacterium solution and contained therein thin
The density of bacterium is controlled by.Volume containing bacterium solution can be 1 10mL.Bacterial density containing bacterium solution can be 102、103、
104、105、106、107Or 108Individual/mL.The volume containing bacterium solution can be fixed, be configured with not according to the difference of total number of bacteria
With bacterial density containing bacterium solution.For example, it is possible to the fixing volume containing bacterium solution is 1mL, thus being configured to density is 102、104
With 108The solution containing bacterium of individual/mL is as standard solution.
In step (4 2), the aperture of microporous filter membrane should be 0.22 μm, and needs in advance through sterilization treatment, in case
Miscellaneous bacteria interference measurement result on microporous membrane surface.
In step (4 2), the filter type used should be as filter type during practical measurement, in order to the greatest extent may be used
Systematic error that is different due to filter type and that cause can be reduced in ground.
In step (4 2), the nucleic acid staining agent used also should be with the kind of nucleic acid staining agent during practical measurement
Equally.
Embodiment one
A kind of method present embodiments providing bacterial density measured in river, it comprises the steps:
(1), obtaining the water sample to be measured of 100mL from river, concussion makes it be evenly distributed, and uses the aperture that sterilization treatment is crossed
It is that the microporous filter membrane of 0.22 μm filters this water sample to be measured in vacuum filtration mode, obtains filtering complete microporous filter membrane, and discard
Filtrate；
(2), being put in filtering complete microporous filter membrane in culture dish, being cut into diameter r with the shears after sterilizing is
The circular filter membrane of 0.5cm, uses the nucleic acid staining agent of 10 μ L to dye circular filter membrane under the conditions of lucifuge 5min, it is thus achieved that after dyeing
Circular filter membrane；
(3), the circular filter membrane after dyeing is placed in 96 orifice plates, uses the exciting light of 470nm to excite at 25 DEG C, use
Microplate reader measures its fluorescence intensity D at 510nmr, then calculate the fluorescence intensity of full wafer microporous filter membraneGlimmering
The result of light intensity is as shown in table 1；
(4), according to the equation of linear regression representing bacterial number and the corresponding relation of fluorescence intensityCalculate the bacterial number of water sample to be measured；
(5) bacterial density of water sample to be measured, is calculated by below equation: the bacterial number of bacterial density=calculate/
The volume of water sample to be measured.
Wherein, in step (4), the construction method of equation of linear regression comprises the steps:
(4 1), acquisition have three solution Han bacterium of different totals number of bacteria；These volumes containing bacterium solution are 1mL,
Density is respectively 104、106、1010Individual/mL.
Three solution Han bacterium are entered by (4 2), the microporous filter membrane that aperture is 0.22 μm using three sterilization treatment to cross respectively
Row filters, and obtains three and filters complete microporous filter membrane, and discards filtrate；
(4 3), filter complete microporous filter membrane to be cut into diameter r respectively by above three with the shears after sterilizing be 0.5cm
Circular filter membrane, use the nucleic acid staining agent of 10 μ L respectively three circular filter membranes to be dyeed under the conditions of lucifuge, obtain three
Circular filter membrane after individual dyeing；
(4 4), the circular filter membrane after blank microporous filter membrane and three dyeing is respectively placed in 96 orifice plates, use
The exciting light of 470nm excites at 25 DEG C, uses microplate reader to measure its fluorescence intensity D at 510nmr', then corresponding calculating
Go out the fluorescence intensity of full wafer microporous filter membraneThe measurement result of fluorescence intensity is as shown in table 1；
(4 5), fluorescence intensity with fluorescence intensity as abscissa, with total number of bacteria as vertical coordinate, to each microporous filter membrane
Carry out curve fitting with the corresponding relation of total number of bacteria, obtain linear fit curve and obtain the equation of linear regression of correspondence:
Wherein, in step (4 1), Flow cytometry can be used first with the antibacterial in centrifuge separation river
Method or blood cell counting obtain different totals number of bacteria, then use ultra-pure water be prepared as volume be 1mL, density is respectively
104、106、1010Individual/mL containing bacterium solution.
Step (4 1) to (4 4) Tong Bu can be carried out with step (1) to (3).
The measurement result table of the fluorescence intensity of table 1 embodiment one
As shown in Table 1, the fluorescence intensity of the microporous filter membrane of water sample to be measured is 4.83E+08, is substituted into linear regression side
Journey, obtaining total number of bacteria is 7083200, and the volume of water sample to be measured is 100mL, and the bacterial density of water sample the most to be measured is 70832
Individual/mL.
Embodiment two
A kind of method present embodiments providing bacterial density measuring tank water body, it comprises the steps:
(1), obtaining the water sample to be measured of 1000mL from tank, concussion makes it be evenly distributed, and uses sterilization treatment to cross
Aperture is that the microporous filter membrane of 0.22 μm filters this water sample to be measured in vacuum filtration mode, obtains filtering complete microporous filter membrane, and
Discard filtrate；
(2), being put in filtering complete microporous filter membrane in culture dish, being cut into diameter r with the shears after sterilizing is
The circular filter membrane of 0.5cm, uses the nucleic acid staining agent of 10 μ L to dye circular filter membrane under the conditions of lucifuge 5min, it is thus achieved that after dyeing
Circular filter membrane；
(3), the circular filter membrane after dyeing is placed in 96 orifice plates, uses the exciting light of 470nm to excite at 25 DEG C, use
Microplate reader measures its fluorescence intensity D at 510nmr, then calculate the fluorescence intensity of full wafer microporous filter membraneGlimmering
The measurement result of light intensity is as shown in table 2；
(4), according to the equation of linear regression representing bacterial number and the corresponding relation of fluorescence intensityCalculate the bacterial number of water sample to be measured；
(5) bacterial density of water sample to be measured, is calculated by below equation: the bacterial number of bacterial density=calculate/
The volume of water sample to be measured.
Wherein, in step (4), the construction method of equation of linear regression comprises the steps:
(4 1), acquisition have three solution Han bacterium of different totals number of bacteria；These volumes containing bacterium solution are 1mL,
Density is respectively 102、104、108Individual/mL.
Three solution Han bacterium are entered by (4 2), the microporous filter membrane that aperture is 0.22 μm using three sterilization treatment to cross respectively
Row filters, and obtains three and filters complete microporous filter membrane, and discards filtrate；
(4 3), filter complete microporous filter membrane to be cut into diameter r respectively by above three with the shears after sterilizing be 0.5cm
Circular filter membrane, use the nucleic acid staining agent of 10 μ L respectively three circular filter membranes to be dyeed under the conditions of lucifuge, obtain three
Circular filter membrane after individual dyeing；
(4 4), the circular filter membrane after blank microporous filter membrane and three dyeing is respectively placed in 96 orifice plates, use
The exciting light of 470nm excites at 25 DEG C, uses microplate reader to measure its fluorescence intensity D at 510nmr', then corresponding calculating
Go out the fluorescence intensity of full wafer microporous filter membraneThe measurement result of fluorescence intensity is as shown in table 2；
(4 5), fluorescence intensity with fluorescence intensity as abscissa, with total number of bacteria as vertical coordinate, to each microporous filter membrane
Carry out curve fitting with the corresponding relation of total number of bacteria, obtain linear fit curve and obtain the equation of linear regression of correspondence:
Wherein, in step (4 1), flow cytometer meter can be used first with the antibacterial in centrifuge separation tank
Number methods or blood cell counting obtain different total number of bacteria, then use ultra-pure water be prepared as volume be 1mL, density is respectively
102、104、108Individual/mL containing bacterium solution.
Step (4 1) to (4 4) Tong Bu can be carried out with step (1) to (3).
The measurement result table of the fluorescence intensity of table 2 embodiment two
As shown in Table 1, the fluorescence intensity of the microporous filter membrane of water sample to be measured is 2.23E+05, is substituted into linear regression side
Journey, obtaining total number of bacteria is 1469, and the volume of water sample to be measured is 1000mL, the bacterial density of water sample the most to be measured is 1.5/
mL。
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and use this
Bright.These embodiments obviously easily can be made various amendment by person skilled in the art, and described herein
General Principle is applied in other embodiments without through performing creative labour.Therefore, the invention is not restricted to above-described embodiment,
Those skilled in the art should be at this according to the announcement of the present invention, the improvement made without departing from scope and amendment
Within bright protection domain.
Claims (6)
1. one kind measures the method for bacterial density in water sample, it is characterised in that: comprise the steps:
Use filtering with microporous membrane water sample to be measured, use nucleic acid staining agent to enter under the conditions of lucifuge filtering complete microporous filter membrane
Row dyeing, and measure the fluorescence intensity of the microporous filter membrane after dyeing, then corresponding with fluorescence intensity according to illustrating bacterial number
The equation of linear regression of relation calculates the bacterial number of described water sample to be measured, and bacterial number with the business of the volume of water sample to be measured is
Bacterial density.
Method the most according to claim 1, it is characterised in that: the aperture of described microporous filter membrane is 0.22 μm.
Method the most according to claim 1, it is characterised in that: described nucleic acid staining agent is9green
fluorescent nucleic acid stains(Molecular Probes,Leiden,Netherlands)。
Method the most according to claim 1, it is characterised in that: the assay method of described fluorescence intensity is: use at 25 DEG C
Microporous filter membrane after dyeing described in the excitation light irradiation of 470nm, then measures its fluorescence intensity at 510nm.
Method the most according to claim 1, it is characterised in that: the construction method of described equation of linear regression includes walking as follows
Rapid:
(1), obtain multiple have different totals number of bacteria containing bacterium solution；
(2), use multiple described microporous filter membrane that multiple solution one_to_one corresponding containing bacterium are filtered；
(3) microporous filter membrane after, using described nucleic acid staining agent to filter each dyes under the conditions of lucifuge；
(4) fluorescence intensity of the microporous filter membrane after blank microporous filter membrane and all of dyeing, is measured；
(5), the fluorescence intensity of each microporous filter membrane and the relation of total number of bacteria are carried out curve fitting, obtain linear fit curve
And obtain the equation of linear regression of correspondence.
Method the most according to claim 5, it is characterised in that: the described bacterial density containing bacterium solution is 102、103、104、
105、106、107Or 108Individual/mL, volume is 1 10mL.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113005172A (en) * | 2021-02-02 | 2021-06-22 | 江苏省农业科学院 | Method for rapidly measuring bacterial quantity of water body |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1680805A (en) * | 2004-04-08 | 2005-10-12 | 广东省微生物研究所 | Rapid microbiological detection and reagent for environmental water body |
| CN101344476A (en) * | 2008-08-20 | 2009-01-14 | 李影 | Fluorescence microscope viewing and counting method for bacteria in viable but non-culturable state |
| CN104593475A (en) * | 2015-02-03 | 2015-05-06 | 武汉市环境保护科学研究院 | Fluorescent microscopic counting method for detecting number of bacteria in water body |
-
2016
- 2016-08-15 CN CN201610667993.7A patent/CN106153591A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1680805A (en) * | 2004-04-08 | 2005-10-12 | 广东省微生物研究所 | Rapid microbiological detection and reagent for environmental water body |
| CN101344476A (en) * | 2008-08-20 | 2009-01-14 | 李影 | Fluorescence microscope viewing and counting method for bacteria in viable but non-culturable state |
| CN104593475A (en) * | 2015-02-03 | 2015-05-06 | 武汉市环境保护科学研究院 | Fluorescent microscopic counting method for detecting number of bacteria in water body |
Non-Patent Citations (1)
| Title |
|---|
| 刘志远 等: "环境水体微生物快速检测方法", 《全国给水深度处理研究会 2015年年会》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113005172A (en) * | 2021-02-02 | 2021-06-22 | 江苏省农业科学院 | Method for rapidly measuring bacterial quantity of water body |
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Application publication date: 20161123 |