CN106148422A - Improve anaerobic fermentation of kitchen waste salt resistance ability and the method for gas generation property - Google Patents
Improve anaerobic fermentation of kitchen waste salt resistance ability and the method for gas generation property Download PDFInfo
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- CN106148422A CN106148422A CN201610553288.4A CN201610553288A CN106148422A CN 106148422 A CN106148422 A CN 106148422A CN 201610553288 A CN201610553288 A CN 201610553288A CN 106148422 A CN106148422 A CN 106148422A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The present invention provides a kind of method improving anaerobic fermentation of kitchen waste salt resistance ability and gas generation property, the method adds glycine betaine complexing agent in anaerobic fermentation of kitchen waste system, maintain the microbial cell osmotic pressure constantly increased because of salt accumulation, reduce the salinity suppression to microbial activity, raising system salt resistance ability, thus improve methane yield, shorten the response time, improve changing food waste utilization ratio.
Description
Technical field
The method that the present invention relates to anaerobic fermentation of kitchen waste, particularly relates to a kind of glycine betaine raising changing food waste that adds and detests
Aerobe fermentation salt resistance ability and the method for gas generation property.
Background technology
Changing food waste refers to residential quarter, business, the food garbage produced for source such as machine-operated and industrial.Along with economy
Development and the raising of living standards of the people so that China's changing food waste generation amount presents the trend risen year by year, current year produces
Amount has reached more than 60,000,000 tons, as a example by Beijing, reaches every day more than 2000 ton the end of the year 2015.Have aqueous for changing food waste
The characteristics such as rate is high, content of organics is high, anaerobic fermentation is one of appropriate technology of changing food waste process, thus can not only eliminate
The environmental pollution brought, the most also realizes changing food waste into resources recycling.
Different from general anaerobic fermentation raw material such as human and animal excreta, straw etc., changing food waste salt content is high, in changing food waste
Inorganic salts includes calcium, magnesium, potassium, ferrum, sodium etc., and wherein the content of sodium salt is the highest.China's changing food waste salinity 2000～
6000mg/L(Na+Concentration) between, and constantly accumulate in continuous print anaerobic fermentation process Zhong Yan branch, this feature can be serious
The efficient stable affecting anaerobic fermentation of kitchen waste system runs, thus limits the application of anaerobic fermentation of kitchen waste.Document: Jiang
Refined. changing food waste Chinese anaerobic digestion process performance and feature analysis of Influential Factors [D]. Southeast China University's .2009. literary composition is studied
Claim, for the anaerobic fermentation of changing food waste, work as Na+When concentration is 3000mg/L, anaerobic fermentation of kitchen waste process is begun with
Inhibitory action, and Na+Concentration more high toxicity is the strongest.Document: Iacovidou E, Ohandja D G, Voulvoulis N.Food
waste disposal units in UK households:The need for policy intervention[J]
.Science of the Total Environment, in 2012,423 (8): 1 7. literary compositions, research claims, and detests for changing food waste
Aerobe fermentation, works as Na+Concentration more than 5000mg/L time, final methane can be converted and have a negative impact.
Why salinity is too high can produce inhibitory action to the growth of microorganism, main reason is that: salinity is too high
Time, cause Premeabilisation of cells pressure drastically to raise, hyperosmosis can make microbial cell dehydration cause cellular plasm to separate, high salt feelings
Dehydrogenase activity is made to reduce because of salting out under condition.
In the face of hyperosmosis environment, most of salt-durable microbes are by synthesizing and accumulation compatible solute in intracellular
(Compatible solutes) regulates the change of osmotic pressure and maintains the normal metabolic activity of cell.Compatible material is usual
For polarity, readily soluble, uncharged small molecule organic material in physiological pH range, wherein, N, N, Betaine
(Betaine) i.e. glycine betaine is a kind of highly effective, compatible solute of serving many purposes.Document: Zhang Yujin, Gao Shiyong,
He Liwei. the bioactivity research [J] of glycine betaine. Harbin University of Commerce's journal: natural science edition, 2006,22:13-16. it
The most important osmotic pressure buffer substances such as microorganism, plant, aquatic organism, osmotic pressure stress time to maintain normal cell metabolism
Play an important role, improve the salt resistant character of cell, the integrity of protection plasma membrane.Glycine betaine is nontoxic Cytoplasm Osmoprotectants,
The exogenous betaine of very low concentrations has good Osmoregulation protective effect.Document: Xing Jin. glycine betaine and the mechanism of action thereof
[J]. Biology Teaching, 2010,35:2-4. glycine betaine increasingly receives publicity as the efficient compatible solute of one.
Summary of the invention
For solving the problems referred to above, the invention provides a kind of a small amount of multiple by adding during anaerobic fermentation of kitchen waste
Join the glycine betaine complexing agent of potassium ion, play glycine betaine in the collaborative lower effectively regulation diafiltration pressure of sodiumpotassium pump (also known as na-k-atp enzyme)
Comprehensive function, increase system salt resistance ability, reduce the salinity suppression to anaerobic fermentation of kitchen waste, to reach to improve methane
The purpose of gas production.
Specifically comprise the following steps that
(1) changing food waste pre-treatment: carry out sorting and break process by the changing food waste of collection.
(2) anaerobic fermentation: be placed in slaking apparatus, according in reaction system through changing food waste and the seed sludge of pre-treatment
Na+Concentration adds the glycine betaine complexing agent of a certain amount of compounding potassium ion, adds water to certain volume, salt concentration in reaction system
For 3g/L (with Na+Meter) more than, control anaerobic fermentation temperature；Finally draw off biogas residue.
Wherein, described changing food waste uses crusher in crushing to become particle diameter about 5～the slurry of about 10mm.
Wherein, described seed sludge is the anaerobic fermentation mud of the anaerobic fermentation mud of various raw material, preferably sewage treatment plant.
Wherein, described anaerobic fermentation of kitchen waste device is homogenous anaerobic fermentation reactor, and the reaction method of operation is permissible
That batch is run: reaction raw materials is disposably put into, confined reaction after inoculation until degradable, final disposable discharging.Fortune
Line mode can also be semi continuous: fixed time intervals adds certain virgin material, discharges certain biogas slurry and biogas residue simultaneously, it is ensured that
Overall balance in system.
Wherein, described changing food waste feed loading is 1.5g (VS)/more than L, and the feed loading of semi-continuous operation is excellent
Select 1.5～5.0g (VS)/L, feed loading preferably 5.0～10g (VS)/L that batch is run.VS represents volatile solid
(Volatile Solid), L represents changing food waste and seed sludge mixeding liquid volume.
Changing food waste adds with seed sludge F/M=0.5 in mass ratio～1.5, preferably F/M=1.F/M represents organic negative
Lotus rate (Food/Microorganism).
Wherein, the addition of the glycine betaine complexing agent of described compounding potassium ion is calculated by Na ion concentration in system, with
Time consider every gram of sodium ion in changing food waste and the factor of seed sludge mixed liquid concentration, changing food waste and seed sludge mixed liquor
Adding glycine betaine 0.05-0.8g, preferably scope is 0.2-0.5g；In changing food waste and seed sludge mixed liquor, every gram of sodium ion adds
Adding potassium ion 0.05～0.5g, preferably scope is 0.1～0.25g.
Wherein, described changing food waste and the mixed liquor of seed sludge and the mass ratio of water, contain solid by total system in reactor
Rate be 5～10% calculate obtain.Solid content refers to the percentage by weight of solid component content in the mixture of anaerobic fermentation system.
Wherein, described anaerobic fermentation temperature is 33～39 DEG C, preferably 35～37 DEG C.
Whole anaerobic fermentation process needs 28～35 days.
After adding the glycine betaine complexing agent of compounding potassium ion, high salt anaerobic fermentation of kitchen waste total gas production less adds
Add glycine betaine complexing agent high salt anaerobic fermentation of kitchen waste total gas production improve more than 25%, methane content be 65%～
78%, average content is 72%.System stability index is all in normal range.
Compared with prior art, the invention has the beneficial effects as follows:
Compared to without glycine betaine complexing agent, the present invention is remarkably improved anaerobic fermentation of kitchen waste total gas production, and
Ensure stable higher methane content.Source benefit and economic benefit that changing food waste into resources processes can be increased.
Compared to without glycine betaine complexing agent, the present invention can alleviate high concentration Na+Anaerobic fermentation of kitchen waste is pressed down
Make use, strengthen system salt resistance ability, improve changing food waste utilization ratio.The present invention can alleviate Na in system longtime running+Long-pending
The tired anaerobic fermentation system performance degradation phenomenon caused；
Detailed description of the invention
Embodiment
The changing food waste food waste disposer of collection is ground into particle diameter about 5～the slurry of about 10mm；Inoculation
After mud stands 1 day, outwell supernatant, take sediment fraction.
Anaerobic ferment devices be volume be the anaerobic reactor of 250mL, effective volume is 150mL, is placed in constant water bath box
In, keep anaerobic fermentation temperature 35 DEG C ± 2 DEG C, the every day of vibration twice sooner or later, each 30 minutes, use drainage to take gas, reaction
Device keeps good anaerobic environment.
Add 1.5gVS kitchen, 1.5gVS seed sludge, add NaCl (Na+For 5.0g/L), in 300mg glycine betaine to reactor,
Inject deionized water to demarcate to 150mL.Stir and be placed in water bath with thermostatic control, stable operation about 30 days at (35 ± 2) DEG C.
Add 1.5gVS kitchen, 1.5gVS seed sludge, add NaCl (Na+For 10.0g/L), compounding potassium ion 100mg
Glycine betaine complexing agent 375mg in reactor, inject deionized water and demarcate to 150mL.Stir and be placed on water bath with thermostatic control
In, stable operation about 35 days at (35 ± 2) DEG C.
Add the anaerobic fermentation of kitchen waste usefulness after glycine betaine complexing agent and be listed in table 1.As can be seen from Table 1, add sweet
After dish alkali complexing agent, Na+For in the anaerobic fermentation of kitchen waste system of 5.0g/L, load methane production is relatively multiple without glycine betaine
Mixture adds 25.00%, Na+For in the anaerobic fermentation of kitchen waste system of 10.0g/L, load methane production is relatively without sweet
Dish alkali adds 27.07%, and two groups of methane content meansigma methodss are the highest.After reaction terminates, the Na in system+Concentration all has little
Amplitude declines, and the test group of interpolation glycine betaine complexing agent is compared with the matched group without adding, and declines more notable.
From in table 1, along with Na+Increasing of concentration, system is elongated from the time brought into operation to stable, Na+For
During 5.0g/L, system was foreshortened to 7 days by 9 days by the test group adding glycine betaine complexing agent from bringing into operation to the stable time,
Total reaction time was foreshortened to 30 days by 35 days；Na+During for 10.0g/L, add glycine betaine test group by system from start fortune
Walking to the stable time was foreshortened to 8 days by 12 days, and total reaction time was foreshortened to 32 days by 38 days；It is therefore seen that add glycine betaine energy
Enough shorten microorganism and adapt to the time of hypersaline environment, strengthen system salt resistance ability.
Table 1 adds the anaerobic fermentation of kitchen waste usefulness after glycine betaine complexing agent
Claims (9)
1. the method improving anaerobic fermentation of kitchen waste salt resistance ability and gas generation property, it is characterised in that include following step
Rapid:
(1) carry out sorting and break process by the changing food waste of collection；
(2) treated changing food waste and seed sludge are placed in slaking apparatus, add compounding according to the Na+ concentration in reaction system
The glycine betaine complexing agent of potassium ion, adds water to certain volume, and in reaction system, salinity is calculated as more than 3g/L with Na+ concentration, controls
Anaerobic fermentation temperature；Finally draw off biogas residue；
The addition of described glycine betaine complexing agent is calculated by Na ion concentration in system, mixes according to changing food waste and seed sludge
In liquid, every gram of sodium ion correspondence adds glycine betaine 0.05-0.8g, corresponding interpolation potassium ion 0.05-0.5g；
The described anaerobic fermentation of kitchen waste reaction method of operation can be that batch is run or semi-continuous operation；
Described anaerobic fermentation temperature is 33～39 DEG C；
Described whole anaerobic fermentation time 28～35 days.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described changing food waste processes and uses crusher in crushing to become particle diameter about 5～the slurry of 10mm.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described seed sludge is the anaerobic fermentation mud of the anaerobic fermentation mud of various raw material, preferably sewage treatment plant.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, the mass ratio of changing food waste and seed sludge is pressed F/M=0.5～1.5 and is added.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, wherein, described changing food waste feed loading is 1.5g (VS)/more than L, and the changing food waste of described semi-continuous operation enters
Material load is 1.5～5.0g (VS)/L, changing food waste feed loading 5.0～10g (VS)/L that batch is run.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described changing food waste and the mixed liquor of digested sludge and the mass ratio of water, be 5 by total system solid content in reactor
～10% percetage by weight.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, the addition of described glycine betaine complexing agent adds according to every gram of sodium ion correspondence in changing food waste and seed sludge mixed liquor
Glycine betaine 0.2-0.5g, corresponding interpolation potassium ion 0.1～0.25g.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described anaerobic fermentation temperature is 35～37 DEG C.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 4 and the method for gas generation property, its feature
Being, the mass ratio of described changing food waste and seed sludge presses F/M=1.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113044978A (en) * | 2021-03-31 | 2021-06-29 | 河南农业大学 | Method for improving anaerobic digestion efficiency of organic wastewater and application thereof |
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| CN103725715A (en) * | 2013-12-30 | 2014-04-16 | 北京化工大学 | Preparation method of calcium ion slow-release agent and method for improving anaerobic digestion of kitchen wastes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103725715A (en) * | 2013-12-30 | 2014-04-16 | 北京化工大学 | Preparation method of calcium ion slow-release agent and method for improving anaerobic digestion of kitchen wastes |
Non-Patent Citations (2)
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| 何健: "甜菜碱和四氢嘧啶提高Pseudomonas putida KT2440耐盐性的分子机理研究", 《中国博士论文全文数据库》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113044978A (en) * | 2021-03-31 | 2021-06-29 | 河南农业大学 | Method for improving anaerobic digestion efficiency of organic wastewater and application thereof |
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Application publication date: 20161123 |