CN106148161B - A kind of reagent cartridge being provided with magnetic bead transfer organization - Google Patents
A kind of reagent cartridge being provided with magnetic bead transfer organization Download PDFInfo
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- CN106148161B CN106148161B CN201510172049.XA CN201510172049A CN106148161B CN 106148161 B CN106148161 B CN 106148161B CN 201510172049 A CN201510172049 A CN 201510172049A CN 106148161 B CN106148161 B CN 106148161B
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- bead transfer
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Abstract
A kind of reagent cartridge being provided with magnetic bead transfer organization, include at least one magnetic bead transfer assembly, the magnetic bead transfer assembly includes the first cavity being disposed adjacent and the second cavity and the interval region that first cavity and the second cavity is spaced apart, an accommodating chamber is formed in the interval region, is additionally provided between the first cavity and the second cavity and the intracorporal magnetic bead of the first chamber is transferred to the intracorporal magnetic bead transfer organization of the second chamber；Magnetic bead transfer organization magnet, and it is fitted into the first interval body and the second interval body for being set to and in the accommodating chamber and being mutually matched, when magnetic bead shifts, first interval body and the second interval body are successively individually opened.The solution of the present invention makes magnetic bead and is adsorbed in the material to be extracted of magnetic bead sufficiently, efficiently shifted by the way that magnetic bead transfer organization is arranged between adjacent cavity；In addition, not only self structure is simple for reagent cartridge of the invention, but also easy to operate, the complexity of the matched equipment used can be effectively reduced.
Description
Technical field
The preparation of nucleic acid or the protein consumptive material more particularly to one kind used with detection are provided with magnetic bead to turn
Move the reagent cartridge of structure.
Background technique
With the transformation of medical model and the continuous development of personalized medicine, there is an urgent need to quick, accurate for medical test circle
Detection means, Molecular Detection then plays unique advantage.The basis of Molecular Detection be analyze detected person histocyte,
Hair, anticoagulation or dry bloodstain and formaldehyde fixes, gene and its expression product in the tissue of paraffin embedding, by from point
Nucleic acid (DNA and RNA) detection is completed in sub- level, in disease once occurring symptom, sign and biochemical change even not yet occur
Before, detection can accurately just be made.
Currently, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology
Deng.Molecular Detection product is mainly used in the detection and physical examination of the clinical departments such as tumour, infection, heredity, Prenatal Screening
The heart, technical service center, third party testing agency and microbial rapid detection market etc..Currently, blood routine, cell
The measuring means such as, pathology and immunology develop towards automation, integration, standardization direction, but due to Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem.For example, the centrifugal column method used in field of biotechnology or paramagnetic particle method carry out nucleic acid extraction, generally requires and split
Four steps such as solution, combination, rinsing, elution, in addition subsequent making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biological core
The detecting steps such as piece keep the full-automatic instrument of entire " from sample to result " very difficult, single with regard to effective in each step
For the transfer of ingredient, the prior art mainly adopts the modes shifted manually, i.e., by artificial mode by raw material or centre
Product is successively shifted in the reaction vessel of step in successively step and realizing, it is therefore apparent that the hand used in the prior art
Animal material branch mode, it is not only cumbersome, time-consuming and laborious.What is more also resides in whole operation process and be easy to cause pollution,
The purity of extract is influenced, and material is difficult sufficiently, efficiently to be shifted, and influences experimental result.Therefore, in view of Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem, to realize material between each step it is abundant, efficiently shift the structure and function just needed in consumptive material and experimental facilities
Can go up carry out it is various consider, can avoid that such as at high cost, poor sensitivity existing in the prior art, structure is complicated, behaviour
Make the problems such as excessively complicated with associated assay devices.
Summary of the invention
Aiming at the shortcomings in the prior art, present invention solves the technical problem that being that providing one kind is provided with magnetic bead transfer knot
The reagent cartridge of structure makes magnetic bead and is adsorbed in the to be extracted of magnetic bead by the way that magnetic bead transfer organization is arranged between adjacent cavity
Material (protein, nucleic acid) sufficiently, is efficiently shifted.
A kind of reagent cartridge for being provided with magnetic bead transfer organization is provided present invention solves the technical problem that also residing in, not only certainly
Body structure is simple, and easy to operate, can be effectively reduced the complexity of the matched equipment used.
In order to solve the above-mentioned technical problem, the reagent cartridge packet provided in an embodiment of the present invention for being provided with magnetic bead transfer organization
Include comprising at least one magnetic bead transfer assembly, the magnetic bead transfer assembly include the first cavity being disposed adjacent and the second cavity and
By first cavity and the second cavity interval region spaced apart, the interval region is interior to form an accommodating chamber, first chamber
It is additionally provided with one between body and the second cavity, the intracorporal magnetic bead of the first chamber is transferred to the intracorporal magnetic bead transfer organization of the second chamber；
The magnetic bead transfer organization includes the magnet for adsorbing magnetic bead, and chimeric is set in the accommodating chamber and mutual
The first interval body and the second interval body matched, first interval body are set to the position in accommodating chamber close to first cavity
It sets, second interval body is set to the position in accommodating chamber close to second cavity, between first interval body and second
Spacer individually chimeric with accommodating chamber sealing can separate first cavity and the second cavity；
First interval body and the second interval body are disposed adjacent and form an interval body adjacency section in the junction of the two,
The interval body adjacency section includes the second adjacent surface positioned at the first adjacent surface of the first interval body and positioned at the second interval body, institute
The scraping block for being provided on the first adjacent surface and extending the face main body is stated, which suppresses when second adjacent surface, and described
The adjoining gap for accommodating magnetic bead is formed between one adjacent surface and the second adjacent surface；Magnetic bead transfer before, first interval body and
The sealing of second interval body, which is embedded in the accommodating chamber and forms sealed spacer region, separates the first cavity and the second cavity；Magnetic
When pearl shifts, first interval body and the second interval body are successively individually opened, and the first interval body rises along accommodating chamber, and magnetic bead exists
Second adjacent surface is adsorbed under the magneticaction that magnet generates, the second interval body sealing is embedded in the accommodating chamber
Separate first cavity and the second cavity；Then the first interval body declines along accommodating chamber, and magnetic bead is transferred to the neighbour by scraping block
Connect gap；Then the second interval body rise, the first interval body sealing be embedded in the accommodating chamber separate first cavity and
Second cavity, removes magnetic force, and magnetic bead is distributed to the second cavity from the adjacent gap.
As a preferred solution of the present invention, a kind of reagent for being provided with magnetic bead transfer organization that the embodiment of the present invention provides
Cartridge further comprises some or all of following technical characteristic:
Preferably, the spliced eye of the receiving magnet is offered in second interval body, the magnet turns in magnetic bead
Intert when shifting and adsorb magnetic bead in the spliced eye, the magnet exits the spliced eye after completing magnetic bead transfer.
Further, which further includes the fixed frame of the fixed magnetic bead transfer assembly, is set on the fixed frame
It is equipped with fixed detent corresponding with magnetic bead transfer assembly number, the magnetic bead transfer assembly and fixed detent are corresponded and fixed
In in the fixed detent, the two sides of the interval region of the magnetic bead transfer assembly are provided with positioning groove, with magnetic bead transfer group
Clamping spine block is provided in the corresponding fixed detent of part, the clamping spine block inlay card is in fixing the magnetic in the positioning groove
Pearl transfer assembly.
Preferably, the magnet includes two magnetic texures for being respectively arranged at the accommodating chamber periphery, two magnetic junctions
Structure generates contrary magnetic field, the shift direction of the connecting line or joint face of two magnetic texures perpendicular to magnetic bead.
Further, which further includes the fixed frame of the fixed magnetic bead transfer assembly, is set on the fixed frame
It is equipped with fixed detent corresponding with magnetic bead transfer assembly number, the magnetic bead transfer assembly and fixed detent are corresponded and fixed
In in the fixed detent, the two sides of the interval region of the magnetic bead transfer assembly are provided with positioning groove, with magnetic bead transfer group
Clamping spine block is provided in the corresponding fixed detent of part, the clamping spine block inlay card is in fixing the magnetic in the positioning groove
Pearl transfer assembly, the magnetic texure is installed on the clamping spine block or magnetic texure itself is used as the clamping spine block.
More preferably, the fixed detent includes accommodating the slot position of the magnetic bead transfer assembly and positioned at the slot position two sides
Side is positioned, is provided with positioning through hole on the positioning side, the clamping spine block is through the positioning through hole, the clamping spine block
For a bar magnet, the two poles of the earth of magnet are located at the two sides on positioning side, and adjacent magnets are as spine block inlay card in a magnetic bead transfer group
For the positioning groove of part with the fixation magnetic bead transfer assembly, two positioning grooves of corresponding same magnetic bead transfer assembly are two pieces
The opposite pole of magnet.
Preferably, the magnet is permanent magnet or electromagnet.
Preferably, the joining place of first cavity and interval region is set as the contraction structure become narrow gradually, and described
The joining place of two cavitys and interval region is set as the contraction structure become narrow gradually.
Preferably, the scraping block is elastic material.
Preferably, multiple magnetic bead transfer assembly in the reagent cartridge comprising being mutually linked.I.e. the embodiment of the present invention provides
The reagent cartridge for being provided with magnetic bead transfer organization include first, second and third ... ..n cavity, between two adjacent cavitys
It is provided with magnetic bead transfer organization.
The reagent cartridge provided in an embodiment of the present invention for being provided with magnetic bead transfer organization, has the following beneficial effects:
The reagent cartridge provided in an embodiment of the present invention for being provided with magnetic bead transfer organization, it includes one or more magnetic beads
By the way that magnetic bead transfer organization is arranged between neighboring chambers in transfer assembly, the first interval body in the magnetic bead transfer organization and the
Two interval bodies individually chimeric with accommodating chamber sealing can separate first cavity and the second cavity, shift in magnetic bead
When, first interval body and the second interval body are successively individually opened, and the first interval body rises along accommodating chamber, and magnetic bead is produced in magnet
Second adjacent surface is adsorbed under raw magneticaction, the second interval body sealing, which is embedded in the accommodating chamber, separates institute
State the first cavity and the second cavity；Then the first interval body declines along accommodating chamber, and magnetic bead is transferred to the adjacent gap by scraping block；
The second last interval body rises, and the sealing of the first interval body, which is embedded in the accommodating chamber, separates first cavity and the second chamber
Body removes magnetic force, and magnetic bead is distributed to the second cavity from the adjacent gap, to realize magnetic bead and be adsorbed in the to be extracted of magnetic bead
Material (protein, nucleic acid) sufficiently, efficiently shifts.Overcome the extractions of the substances such as nucleic acid, albumen, in detection process each step it
Between due to the linkings operation such as material transfer existing for trivial operations, sample or the problem of reagent vulnerable to pollution.In addition, of the invention
It is provided with the reagent cartridge of magnetic bead transfer organization, not only self structure is simple, but also easy to operate, due in magnetic bead transfer organization
The first interval body and the second interval body ingehious design, as long as the first interval body of longitudinal push-and-pull and the second interval body, can make
First interval body and the second interval body are successively individually opened, and by magnetic bead and are adsorbed in material to be extracted (protein, core of magnetic bead
Acid) it is shifted between neighboring chambers, and can effectively avoid the mutually dry in this transfer process of unclassified stores between neighboring chambers
It disturbs, causes unnecessary pollution, influence the purity and quality of extracting final product.Further, in the ancillary equipment of periphery simultaneously
It does not need to be arranged to transport exclusively for magnetic bead and the slewing of rotary power is provided, it is only necessary to provide the power source longitudinally pushed and pulled i.e.
Can, to significantly reduce the complexity of the matched equipment used.
Detailed description of the invention
Fig. 1 is the magnetic bead transfer assembly for the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structural schematic diagram one.
Fig. 2 is the magnetic bead transfer assembly for the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structural schematic diagram two.
Fig. 3 is the cavity body structure in the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structural schematic diagram.
Fig. 4 is the first interval body in the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structural schematic diagram.
Fig. 5 is the second interval body in the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structural schematic diagram.
Fig. 6 is the knot of the fixed frame in the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structure schematic diagram.
Fig. 7 is the structural schematic diagram for the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides.
Specific embodiment
It includes: the transfer of at least one magnetic bead that the embodiment of the present invention, which provides a kind of reagent cartridge for being provided with magnetic bead transfer organization,
Component, the magnetic bead transfer assembly include the first cavity being disposed adjacent and the second cavity and by first cavity and the second chamber
Body interval region spaced apart, the interval region is interior to form an accommodating chamber, also sets up between first cavity and the second cavity
Have one the intracorporal magnetic bead of first chamber is transferred to the intracorporal magnetic bead transfer organization of the second chamber；The magnetic bead transfer organization includes absorption
The magnet of magnetic bead, and it is fitted into the first interval body and the second interval body for being set to and in the accommodating chamber and being mutually matched, it is described
First interval body is set to the position in accommodating chamber close to first cavity, and second interval body, which is set in accommodating chamber, to be leaned on
The position of nearly second cavity, first interval body and the second interval body individually chimeric with accommodating chamber sealing can incite somebody to action
First cavity and the second cavity separate；First interval body and the second interval body are disposed adjacent and in the junctions of the two
An interval body adjacency section is formed, the interval body adjacency section includes positioned at the first adjacent surface of the first interval body and between second
Second adjacent surface of spacer is provided with the scraping block for extending the face main body on first adjacent surface, which suppresses in described
When the second adjacent surface, the adjoining gap for accommodating magnetic bead is formed between first adjacent surface and the second adjacent surface；Magnetic bead transfer
Before, first interval body and the sealing of the second interval body are embedded in the accommodating chamber and form sealed spacer region for the first chamber
Body and the second cavity separate；When magnetic bead shifts, first interval body and the second interval body are successively individually opened, the first interval body
Rise along accommodating chamber, magnetic bead is adsorbed to second adjacent surface under the magneticaction that magnet generates, and second interval body is close
Envelope, which is embedded in the accommodating chamber, separates first cavity and the second cavity；Then the first interval body declines along accommodating chamber, scrapes
Magnetic bead is transferred to the adjacent gap by block；Then the second interval body rises, and the sealing of the first interval body is embedded in the accommodating chamber
First cavity and the second cavity are inside separated, magnetic force is removed, magnetic bead is distributed to the second cavity from the adjacent gap.
Wherein, the spliced eye of the receiving magnet is offered in second interval body, the magnet is shifted in magnetic bead
When intert and adsorb magnetic bead in the spliced eye, the magnet exits the spliced eye after completing magnetic bead transfer.
Wherein, which further includes the fixed frame of the fixed magnetic bead transfer assembly, is provided on the fixed frame
Fixed detent corresponding with magnetic bead transfer assembly number, the magnetic bead transfer assembly and fixed detent correspond and are fixed on institute
It states in fixed detent, the two sides of the interval region of the magnetic bead transfer assembly are provided with positioning groove, with magnetic bead transfer assembly phase
It is provided with clamping spine block in corresponding fixed detent, the clamping spine block inlay card is in fixing the magnetic bead turn in the positioning groove
Move component.
Wherein, the magnet includes two magnetic texures for being respectively arranged at the accommodating chamber periphery, two magnetic texures
Generate contrary magnetic field, the shift direction of the connecting line or joint face of two magnetic texures perpendicular to magnetic bead.
Wherein, which further includes the fixed frame of the fixed magnetic bead transfer assembly, is provided on the fixed frame
Fixed detent corresponding with magnetic bead transfer assembly number, the magnetic bead transfer assembly and fixed detent correspond and are fixed on institute
It states in fixed detent, the two sides of the interval region of the magnetic bead transfer assembly are provided with positioning groove, with magnetic bead transfer assembly phase
It is provided with clamping spine block in corresponding fixed detent, the clamping spine block inlay card is in fixing the magnetic bead turn in the positioning groove
Component is moved, the magnetic texure is installed on the clamping spine block or magnetic texure itself is used as the clamping spine block.
Wherein, the fixed detent includes accommodating the slot position of the magnetic bead transfer assembly and determining positioned at the slot position two sides
Position side, is provided with positioning through hole on the positioning side, the clamping spine block is through the positioning through hole, the clamping spine block
One bar magnet, the two poles of the earth of magnet are located at the two sides on positioning side, and adjacent magnets are as spine block inlay card in a magnetic bead transfer assembly
Positioning groove with the fixation magnetic bead transfer assembly, two positioning grooves of corresponding same magnetic bead transfer assembly are two blocks of magnetic
The opposite pole of iron.
Wherein, the magnet is permanent magnet or electromagnet.
Wherein, the joining place of first cavity and interval region is set as the contraction structure become narrow gradually, and described second
The joining place of cavity and interval region is set as the contraction structure become narrow gradually.
Wherein, the scraping block is elastic material.
Wherein, multiple magnetic bead transfer assembly in the reagent cartridge comprising being mutually linked.It is i.e. provided in an embodiment of the present invention
The reagent cartridge for being provided with magnetic bead transfer organization includes first, second and third ..., and ..n cavity is set between two adjacent cavitys
It is equipped with magnetic bead transfer organization.
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1,2 and 7 are that the structure for the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides is shown
It is intended to.The reagent cartridge for being provided with magnetic bead transfer organization is applied in nucleic acid or albumen preparation and detection device, it is intended to be core
Material transfer in acid or albumen preparation and detection process between adjacent step provide it is a kind of efficiently, efficiently solution, but
It is not limited thereto, in practical applications, the box that extracts can also be applied to the extraction of other biological extract.
As shown in figs. 1-7, the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides is provided with
Magnetic bead transfer assembly 100, the magnetic bead transfer assembly 100 include the first cavity 10 for being disposed adjacent and the second cavity 20 and by the
The interval region 30 that one cavity 10 and the second cavity 20 are spaced apart.It is shown from the structure in Fig. 1,2 and 7, in implementation of the invention
The first cavity 10 and the second cavity 20 are open-type structure in example, in fact, this is only a kind of implementation of the invention,
In other embodiments of the invention, the first cavity 10 and the second cavity 20 can also be set as needed in addition to interval region
Outside the position communicated, other each faces are closed cavity body structure, and work as the examination for being provided with magnetic bead transfer organization of the invention
When agent cartridge is used in the extraction and test experience of nucleic acid, other than the position communicated with interval region, other each faces are closing
Cavity body structure be more commonly used structure.
When specific implementation, an accommodating chamber 31 is formed in the interval region 30, between the first cavity 10 and the second cavity 20 also
Be provided with a magnetic bead transfer organization 40 magnetic bead in the first cavity 10 being transferred in second cavity 20, magnetic bead transfer organization 40
Sealing is embedded in 31 in accommodating chamber.Wherein, magnetic bead transfer organization 40 includes the magnet (not shown) of absorption magnetic bead, Yi Jiqian
Close the first interval body 41 and the second interval body 42 for being set to and in accommodating chamber 31 and being mutually matched.During specific implementation, first
Interval body 41 is set to the position in accommodating chamber 31 close to the first cavity 10, and the second interval body 42 is set to close in accommodating chamber 31
The position of second cavity 20, the first interval body 41 and the second interval body 42 can be individually chimeric with the sealing of accommodating chamber 31 by the first chamber
Body 10 and the second cavity 20 separate.
In a preferred embodiment of the invention, the first interval body 41 and the second interval body 42 are disposed adjacent and in the knot of the two
An interval body adjacency section is formed at conjunction, interval body adjacency section includes being located at the first adjacent surface 411 of the first interval body 41 and being located at
Second adjacent surface 421 of the second interval body 42 is provided with the scraping block 412 for extending the face main body on first adjacent surface 411, this is scraped
Block 412 suppresses when the second adjacent surface 421, and the neighbour for accommodating magnetic bead is formed between the first adjacent surface 411 and the second adjacent surface 421
Connect gap 43.It is, in principle, that the present invention be mainly by using including magnetic bead including magnetic-particle as extract nucleic acid,
Albumen or other macromolecular bio-extracts, magnetic bead are realized in the first cavity 10 to nucleic acids in samples, albumen or other macromoleculars
The suction-operated of bio-extract, be adsorbed on the magnetic bead in the first cavity 10 in mixed material nucleic acid to be extracted, albumen or
Other macromolecular bio-extracts.At this point, the first interval body 41 and the sealing of the second interval body 42 are embedded in appearance i.e. before magnetic bead transfer
It receives in chamber 31 and forms sealed spacer region 30 and separate the first cavity 10 and the second cavity 20；When magnetic bead shifts, the first interval
Body 41 and the second interval body 42 are successively individually opened, detailed process are as follows: firstly, the first interval body 41 rises along accommodating chamber 31, magnetic
Pearl is adsorbed to the second adjacent surface 421 under the magneticaction that magnet generates, and the sealing of the second interval body 42 is embedded in accommodating chamber 31
Separate the first cavity 10 and the second cavity 20.Then, the first interval body 41 declines along accommodating chamber 31, and scraping block 412 shifts magnetic bead
To adjacent gap 43.Finally, the second interval body 42 rises, the sealing of the first interval body 41, which is embedded in accommodating chamber 31, separates the first chamber
Body 10 and the second cavity 20, remove magnetic force, and magnetic bead is distributed to the second cavity 20 from adjacent gap 43.It is real by above-mentioned transfer process
Existing magnetic bead and the material to be extracted (protein, nucleic acid) for being adsorbed in magnetic bead sufficiently, efficiently shift.Overcome the objects such as nucleic acid, albumen
In the extraction of matter, detection process between each step due to the linkings operation such as material transfer existing for trivial operations, sample or reagent
The problem of vulnerable to pollution.In addition, the reagent cartridge for being provided with magnetic bead transfer organization of the invention, not only self structure is simple, and
And it is easy to operate, due to the ingehious design of the first interval body and the second interval body in magnetic bead transfer organization, as long as longitudinal push-and-pull
First interval body and the second interval body can be such that the first interval body and the second interval body successively individually opens, by magnetic bead and absorption
Transfer is oriented between neighboring chambers in the material to be extracted (protein, nucleic acid) of magnetic bead, ensure that other objects in addition to magnetic bead
Expect the mutual independence between the first cavity and the second cavity, effectively avoids the transfer herein of unclassified stores between neighboring chambers
It interferes with each other in the process, causes unnecessary pollution, influence the purity and quality of extracting final product.Further, in periphery
It does not need to be arranged in ancillary equipment to transport exclusively for magnetic bead and the slewing of rotary power is provided, it is only necessary to which longitudinal push-and-pull is provided
Power source, to significantly reduce the complexity of the matched equipment used.
In addition, from the point of view of the structure setting of the first cavity 10 and the second cavity 20 and the joining place of interval region, in order to
Enough sufficiently, magnetic bead is efficiently shifted, the joining place of the first cavity 10 and interval region 30 is set as the contraction structure become narrow gradually,
In the cohesive position of the first cavity 10 and the first interval body 41, the first cavity 10 is contracted to corresponding with first interval body 41
Width.Correspondingly, the joining place of the second cavity 20 and interval region is also configured as the contraction structure become narrow gradually, second
The cohesive position of cavity 20 and the second interval body 42, the second cavity 20 are contracted to width corresponding with the second interval body 42.Such as Fig. 1
Shown in 3, the joining place of the first cavity 10 and the second cavity 20 and interval region may be configured as the structure that chamfering receives side, certainly
It can be set the width of the first cavity 10 and the second cavity 20 to close to its being connected with interval region using other modes
Structure gradually narrowed during place.
As shown in figs. 1-7, in the preferred embodiment of the present invention 1, the receiving magnetic is offered in the second interval body 42
The spliced eye 422 of body, magnet interts when magnetic bead shifts adsorbs magnetic bead in the spliced eye 422, completes institute after magnetic bead transfer
It states magnet and exits spliced eye 422.In this scheme, the working method of magnetic bead transfer organization 40 are as follows: firstly, the first interval body 41 is along appearance
Chamber 31 of receiving rises, and magnet is inserted into spliced eye 422, magnetic bead is adsorbed to the second adjacent surface under the magneticaction that magnet generates
421, the sealing of the second interval body 42, which is embedded in accommodating chamber 31, separates the first cavity 10 and the second cavity 20.Then, the first interval
Body 41 declines along accommodating chamber 31, and magnetic bead is transferred to adjacent gap 43 by scraping block 412.Finally, magnet is exited spliced eye 422, magnetic
Body disappears to the suction-operated of magnetic bead, and the second interval body 42 rises, and the sealing of the first interval body 41, which is embedded in accommodating chamber 31, to be separated
First cavity 10 and the second cavity 20, magnetic bead are distributed to the second cavity 20 from adjacent gap 43.
In addition, reagent cartridge provided by the embodiment of the present invention 1 further includes the fixed frame of fixed magnetic bead transfer assembly 100
50, it is provided with fixed detent 51 corresponding with 100 numbers of magnetic bead transfer assembly on fixed frame 50, magnetic bead transfer assembly 100 and solid
Determine detent 51 to correspond and be fixed in fixed detent 51, the two sides of the interval region 30 of magnetic bead transfer assembly 100 are provided with
Positioning groove 32, clamping spine block 52 is provided in fixed detent 51 corresponding with magnetic bead transfer assembly 100, and clamping spine block 52 blocks
Magnetic bead transfer assembly 100 is fixed in positioning groove 32.
In the embodiment of the present invention 2, magnet includes two magnetic texures for being respectively arranged at 31 periphery of accommodating chamber, two magnetic
Property structure generates contrary magnetic field, the shift direction of the connecting line or joint face of two magnetic texures perpendicular to magnetic bead.
In the fixed form of fixed magnetic bead transfer assembly 100, used and the embodiment of the present invention in the embodiment of the present invention 2
1 similar mode.As shown in fig. 7, the reagent cartridge that the embodiment of the present invention 2 provides also includes fixed magnetic bead transfer assembly 100
Fixed frame 50 is provided with fixed detent 51 corresponding with 100 numbers of magnetic bead transfer assembly, magnetic bead transfer assembly on fixed frame 50
100 correspond and are fixed in fixed detent 51, the two sides of the interval region 30 of magnetic bead transfer assembly 100 with fixed detent 51
It is provided with positioning groove 32, clamping spine block 52 is provided in fixed detent 51 corresponding with magnetic bead transfer assembly 100, clamps spine
52 inlay card of block is in fixing magnetic bead transfer assembly 100 in positioning groove 32.In the embodiment of the present invention, magnetic texure itself is as clamping
Spine block 52.When specific implementation, fixed detent 51 includes the slot position for accommodating magnetic bead transfer assembly 100 and the positioning positioned at slot position two sides
Side 53 positions and is provided with positioning through hole 54 on side 53, and for clamping spine block 52 through positioning through hole 54, clamping spine block 52 is a bar shaped
Magnet, the two poles of the earth of magnet are located at the two sides on positioning side 53, and adjacent magnets are as spine block inlay card in magnetic bead transfer assembly 100
For positioning groove 32 to fix magnetic bead transfer assembly 100, two positioning grooves 32 of corresponding same magnetic bead transfer assembly 100 are two
The opposite pole of block magnet.In the present solution, the working method of magnetic bead transfer organization 40 are as follows: firstly, the first interval body 41 is along receiving
Chamber 31 rises, and magnetic bead is adsorbed to the second adjacent surface 421 under the magneticaction that magnet generates, and the sealing of the second interval body 42 is embedded in
The first cavity 10 and the second cavity 20 are separated in accommodating chamber 31.Then, the first interval body 41 declines along accommodating chamber 31, scraping block 412
Magnetic bead is transferred to adjacent gap 43.Finally, removing magnet or removing the magnetic force of magnet generation by controlling, the second interval is allowed
Body 42 rises, and the sealing of the first interval body 41 is embedded in accommodating chamber 31 and separates the first cavity 10 and the second cavity 20, and magnetic bead is from neighbour
It connects gap 43 and is distributed to the second cavity 20.Certainly, in other embodiments of the invention, magnetic texure also can according to need only
It is only mounted to clamping spine block 52 or is intended only as clamping 52 1 component parts of spine block, to be able to solve technology of the invention
Problem simultaneously reaches subject to effective magnetic bead adsorption effect.
For magnet selection with from the point of view of material, when specific implementation, magnet is permanent magnet or electromagnet.Of the invention
Permanent magnet or can be extraordinary preferred embodiment by the electromagnet of power control is selected in embodiment 1.And in this hair
In bright embodiment two, magnetism, and the action time in magnetic field easily and efficiently can not only be generated and remove using electromagnet
(i.e. magnetic bead transfer time) can be accurately controlled, and magnetic bead is quick from adjacent gap 43 after magnetic bead is transferred to the second cavity
It is distributed in the second cavity and is further reacted.
From material selection, the main structure that the present invention is provided with the reagent cartridge of magnetic bead transfer organization is all made of plastics
Material is used as reaction consumptive material.Certainly, the magnet used in reagent cartridge using magnetic material commonly used in the art or
The electromagnet that magnetic susceptible material is made into.In addition, for the ease of effectively magnetic bead is scraped between adjoining in magnetic bead transfer process
Gap 43, the scraping block 412 on the first interval body 41 select the better rubber material of elasticity, other can guarantee to scrape off efficiency certainly
Elastic material is also the scheme that can choose in other embodiments of the invention, is fallen within the scope of the present invention.
It should be noted that from Fig. 1,2 and 7 structure can be seen that it is provided in an embodiment of the present invention be provided with magnetic bead turn
The magnetic bead transfer assembly 100 moved in structuring agents cartridge is only to be disposed adjacent comprising the first cavity 10 and the second cavity 20 two
Cavity.In fact, in fact, using the magnetic bead transfer assembly of cavity there are two only as the present invention in the preferred embodiment of the present invention
A kind of preferred embodiment of scheme is to illustrate that the present invention is nucleic acid or albumen preparation and inspection to start with from simple structure
One kind that material transfer during survey between adjacent step provides efficiently, efficiently solution, but be not limited thereto,
In practical application, the material due to being related to multiple steps in nucleic acid extraction and detection process is shifted, even if being related to passing through
Magnetic bead realize material transfer step also have it is multiple, therefore, in other embodiments of the invention in the reagent cartridge include phase
The multiple magnetic bead transfer assembly being mutually connected.That is the reagent cartridge for being provided with magnetic bead transfer organization of other embodiments of the invention offer
The ..n cavity including first, second and third ... is provided with magnetic bead transfer organization between two adjacent cavitys.
Above-described embodiment disclose the reagent cartridge for being provided with magnetic bead transfer organization, it includes one or more magnetic beads turn
Move the first interval body and second by the way that magnetic bead transfer organization is arranged between neighboring chambers in component, in the magnetic bead transfer organization
Interval body individually chimeric with accommodating chamber sealing can separate first cavity and the second cavity, in magnetic bead transfer,
First interval body and the second interval body are successively individually opened, and the first interval body rises along accommodating chamber, and magnetic bead is generated in magnet
Magneticaction under be adsorbed to second adjacent surface, the second interval body sealing be embedded in the accommodating chamber separate it is described
First cavity and the second cavity；Then the first interval body declines along accommodating chamber, and magnetic bead is transferred to the adjacent gap by scraping block；Most
The second interval body rises afterwards, and the sealing of the first interval body, which is embedded in the accommodating chamber, separates first cavity and the second cavity,
Magnetic force is removed, magnetic bead is distributed to the second cavity from the adjacent gap, to realize magnetic bead and be adsorbed in the object to be extracted of magnetic bead
Expect that (protein, nucleic acid) sufficiently, efficiently shifts.Overcome the extractions of the substances such as nucleic acid, albumen, in detection process between each step
The problem of trivial operations existing for due to the linkings operation such as material transfer, sample or reagent vulnerable to pollution.In addition, of the invention sets
It is equipped with the reagent cartridge of magnetic bead transfer organization, not only self structure is simple, but also easy to operate, due in magnetic bead transfer organization
The ingehious design of first interval body and the second interval body can make the as long as the first interval body of longitudinal push-and-pull and the second interval body
One interval body and the second interval body are successively individually opened, and by magnetic bead and are adsorbed in the material to be extracted (protein, nucleic acid) of magnetic bead
It is shifted between neighboring chambers, and can effectively avoid interfering with each other in this transfer process for unclassified stores between neighboring chambers,
Unnecessary pollution is caused, the purity and quality of extracting final product are influenced.Further, periphery ancillary equipment in and be not required to
It is arranged to transport exclusively for magnetic bead and the slewing of rotary power is provided, it is only necessary to the power source longitudinally pushed and pulled is provided, from
And significantly reduce the complexity of the matched equipment used.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (8)
1. a kind of reagent cartridge for being provided with magnetic bead transfer organization includes at least one magnetic bead transfer assembly, the magnetic bead transfer group
Part includes the first cavity being disposed adjacent and the second cavity and the spacer region that first cavity and the second cavity is spaced apart
Domain a, it is characterised in that: accommodating chamber is formed in the interval region, one is additionally provided between first cavity and the second cavity will
The intracorporal magnetic bead of first chamber is transferred to the intracorporal magnetic bead transfer organization of the second chamber；
The magnetic bead transfer organization includes the magnet for adsorbing magnetic bead, and chimeric be set in the accommodating chamber and be mutually matched
First interval body and the second interval body, first interval body are set to the position in accommodating chamber close to first cavity, institute
The position that the second interval body is set to close second cavity in accommodating chamber is stated, first interval body and the second interval body are equal
Individually chimeric with accommodating chamber sealing first cavity and the second cavity can be separated；
First interval body and the second interval body are disposed adjacent and form an interval body adjacency section in the junction of the two, described
Interval body adjacency section includes the first adjacent surface positioned at the first interval body and the second adjacent surface positioned at the second interval body, and described the
The scraping block for extending the face main body is provided on one adjacent surface, which suppresses when second adjacent surface, and described first is adjacent
The adjoining gap for accommodating magnetic bead is formed between junction and the second adjacent surface；Before magnetic bead transfer, first interval body and second
Interval body sealing, which is embedded in the accommodating chamber and forms sealed spacer region, separates the first cavity and the second cavity；Magnetic bead turns
When shifting, first interval body and the second interval body are successively individually opened, and the first interval body rises along accommodating chamber, and magnetic bead is in magnet
Second adjacent surface is adsorbed under the magneticaction of generation, the second interval body sealing, which is embedded in the accommodating chamber, to be separated
First cavity and the second cavity；Then the first interval body declines along accommodating chamber, and magnetic bead is transferred between the adjoining by scraping block
Gap；Then the second interval body rises, and the sealing of the first interval body, which is embedded in the accommodating chamber, separates first cavity and second
Cavity, removes magnetic force, and magnetic bead is distributed to the second cavity from the adjacent gap；
The spliced eye of the receiving magnet is offered in second interval body, the magnet interts when magnetic bead shifts into institute
It states and adsorbs magnetic bead in spliced eye, the magnet exits the spliced eye after completing magnetic bead transfer；
The joining place of first cavity and interval region is set as the contraction structure become narrow gradually, second cavity and interval
The joining place in region is set as the contraction structure become narrow gradually.
2. being provided with the reagent cartridge of magnetic bead transfer organization as described in claim 1, it is characterised in that: the reagent cartridge is also wrapped
The fixed frame of the fixed magnetic bead transfer assembly is included, is provided with fixation corresponding with magnetic bead transfer assembly number on the fixed frame
Detent, the magnetic bead transfer assembly and fixed detent are corresponded and are fixed in the fixed detent, the magnetic bead transfer group
The two sides of the interval region of part are provided with positioning groove, and clamping spine is provided in fixed detent corresponding with magnetic bead transfer assembly
Block, the clamping spine block inlay card is in fixing the magnetic bead transfer assembly in the positioning groove.
3. being provided with the reagent cartridge of magnetic bead transfer organization as described in claim 1, it is characterised in that: the magnet includes two
A magnetic texure for being respectively arranged at the accommodating chamber periphery, two magnetic texures generate contrary magnetic field, two magnetism
Shift direction of the connecting line or joint face of structure perpendicular to magnetic bead.
4. being provided with the reagent cartridge of magnetic bead transfer organization as claimed in claim 3, it is characterised in that: the reagent cartridge is also wrapped
The fixed frame of the fixed magnetic bead transfer assembly is included, is provided with fixation corresponding with magnetic bead transfer assembly number on the fixed frame
Detent, the magnetic bead transfer assembly and fixed detent are corresponded and are fixed in the fixed detent, the magnetic bead transfer group
The two sides of the interval region of part are provided with positioning groove, and clamping spine is provided in fixed detent corresponding with magnetic bead transfer assembly
Block, the clamping spine block inlay card are installed on institute in fixing the magnetic bead transfer assembly, the magnetic texure in the positioning groove
It states clamping spine block or magnetic texure itself is used as the clamping spine block.
5. being provided with the reagent cartridge of magnetic bead transfer organization as claimed in claim 4, it is characterised in that: the fixed detent packet
It includes the slot position for accommodating the magnetic bead transfer assembly and is provided with positioning on the positioning side of the slot position two sides, the positioning side
Through-hole, the clamping spine block is through the positioning through hole, and the clamping spine block is a bar magnet, and it is fixed that the two poles of the earth of magnet are located at
The two sides on position side, positioning groove of the adjacent magnets as spine block inlay card in a magnetic bead transfer assembly are shifted with the fixation magnetic bead
Component, correspond to two positioning grooves of same magnetic bead transfer assembly is the opposite pole of two blocks of magnet.
6. being provided with the reagent cartridge of magnetic bead transfer organization as described in claim 1, it is characterised in that: the magnet is permanent magnetism
Body or electromagnet.
7. being provided with the reagent cartridge of magnetic bead transfer organization as described in claim 1, it is characterised in that: the scraping block is elasticity
Material.
8. being provided with the reagent cartridge of magnetic bead transfer organization as described in claim 1, it is characterised in that: wrapped in the reagent cartridge
Containing the multiple magnetic bead transfer assembly as described in claim 1 being mutually linked.
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| CN201510172049.XA CN106148161B (en) | 2015-04-10 | 2015-04-10 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
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|---|---|---|---|
| CN201510172049.XA CN106148161B (en) | 2015-04-10 | 2015-04-10 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
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| CN106148161B true CN106148161B (en) | 2019-07-02 |
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| CN106148161A (en) | 2016-11-23 |
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