CN106137974A - High activity Phaeoporus obliquus powder and preparation method thereof - Google Patents
High activity Phaeoporus obliquus powder and preparation method thereof Download PDFInfo
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- CN106137974A CN106137974A CN201610698350.9A CN201610698350A CN106137974A CN 106137974 A CN106137974 A CN 106137974A CN 201610698350 A CN201610698350 A CN 201610698350A CN 106137974 A CN106137974 A CN 106137974A
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- phaeoporus obliquus
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- 239000000843 powder Substances 0.000 title claims abstract description 44
- 230000000694 effects Effects 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 238000001816 cooling Methods 0.000 claims abstract description 15
- 239000011261 inert gas Substances 0.000 claims abstract description 15
- 238000010298 pulverizing process Methods 0.000 claims abstract description 14
- 238000002061 vacuum sublimation Methods 0.000 claims abstract description 14
- 239000002245 particle Substances 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 4
- 238000000859 sublimation Methods 0.000 claims abstract description 3
- 230000008022 sublimation Effects 0.000 claims abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 229910000831 Steel Inorganic materials 0.000 claims description 5
- 239000010959 steel Substances 0.000 claims description 5
- 239000007789 gas Substances 0.000 claims description 2
- 239000001307 helium Substances 0.000 claims description 2
- 229910052734 helium Inorganic materials 0.000 claims description 2
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 2
- 235000001553 Betula platyphylla Nutrition 0.000 claims 1
- 241001313086 Betula platyphylla Species 0.000 claims 1
- 230000004071 biological effect Effects 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 description 22
- 238000000034 method Methods 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 9
- 238000012360 testing method Methods 0.000 description 6
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000414067 Inonotus obliquus Species 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000011768 flavin mononucleotide Substances 0.000 description 2
- FVTCRASFADXXNN-SCRDCRAPSA-N flavin mononucleotide Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-SCRDCRAPSA-N 0.000 description 2
- 229940013640 flavin mononucleotide Drugs 0.000 description 2
- FVTCRASFADXXNN-UHFFFAOYSA-N flavin mononucleotide Natural products OP(=O)(O)OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-UHFFFAOYSA-N 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910052756 noble gas Inorganic materials 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 235000019231 riboflavin-5'-phosphate Nutrition 0.000 description 2
- JMSVCTWVEWCHDZ-UHFFFAOYSA-N syringic acid Chemical compound COC1=CC(C(O)=O)=CC(OC)=C1O JMSVCTWVEWCHDZ-UHFFFAOYSA-N 0.000 description 2
- -1 triterpenoid compound Chemical class 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- BQPPJGMMIYJVBR-UHFFFAOYSA-N (10S)-3c-Acetoxy-4.4.10r.13c.14t-pentamethyl-17c-((R)-1.5-dimethyl-hexen-(4)-yl)-(5tH)-Delta8-tetradecahydro-1H-cyclopenta[a]phenanthren Natural products CC12CCC(OC(C)=O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C BQPPJGMMIYJVBR-UHFFFAOYSA-N 0.000 description 1
- CHGIKSSZNBCNDW-UHFFFAOYSA-N (3beta,5alpha)-4,4-Dimethylcholesta-8,24-dien-3-ol Natural products CC12CCC(O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21 CHGIKSSZNBCNDW-UHFFFAOYSA-N 0.000 description 1
- XYTLYKGXLMKYMV-UHFFFAOYSA-N 14alpha-methylzymosterol Natural products CC12CCC(O)CC1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C XYTLYKGXLMKYMV-UHFFFAOYSA-N 0.000 description 1
- FPTJELQXIUUCEY-UHFFFAOYSA-N 3beta-Hydroxy-lanostan Natural products C1CC2C(C)(C)C(O)CCC2(C)C2C1C1(C)CCC(C(C)CCCC(C)C)C1(C)CC2 FPTJELQXIUUCEY-UHFFFAOYSA-N 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 235000009109 Betula pendula Nutrition 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- BKLIAINBCQPSOV-UHFFFAOYSA-N Gluanol Natural products CC(C)CC=CC(C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(O)C(C)(C)C4CC3 BKLIAINBCQPSOV-UHFFFAOYSA-N 0.000 description 1
- LOPKHWOTGJIQLC-UHFFFAOYSA-N Lanosterol Natural products CC(CCC=C(C)C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(C)(O)C(C)(C)C4CC3 LOPKHWOTGJIQLC-UHFFFAOYSA-N 0.000 description 1
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical class O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 1
- CAHGCLMLTWQZNJ-UHFFFAOYSA-N Nerifoliol Natural products CC12CCC(O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C CAHGCLMLTWQZNJ-UHFFFAOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000000498 ball milling Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- QBSJHOGDIUQWTH-UHFFFAOYSA-N dihydrolanosterol Natural products CC(C)CCCC(C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(C)(O)C(C)(C)C4CC3 QBSJHOGDIUQWTH-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 150000002224 folic acids Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- CAHGCLMLTWQZNJ-RGEKOYMOSA-N lanosterol Chemical compound C([C@]12C)C[C@@H](O)C(C)(C)[C@H]1CCC1=C2CC[C@]2(C)[C@H]([C@H](CCC=C(C)C)C)CC[C@@]21C CAHGCLMLTWQZNJ-RGEKOYMOSA-N 0.000 description 1
- 229940058690 lanosterol Drugs 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- YIBXWXOYFGZLRU-UHFFFAOYSA-N syringic aldehyde Natural products CC12CCC(C3(CCC(=O)C(C)(C)C3CC=3)C)C=3C1(C)CCC2C1COC(C)(C)C(O)C(O)C1 YIBXWXOYFGZLRU-UHFFFAOYSA-N 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 description 1
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 240000004494 yellow birch Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a kind of high activity Phaeoporus obliquus powder and preparation method thereof.This preparation method comprises the steps: to be placed in container Phaeoporus obliquus, is filled with liquid inert gas the most in the above-described container, and described Phaeoporus obliquus is 1:1～3 with the weight ratio of liquid inert gas；Standing cooling, the time is 10～30min；Take out through the described Phaeoporus obliquus stood after cooling down, carry out vacuum sublimation and be dried；Phaeoporus obliquus after described sublimation drying is carried out ball mill pulverizing, obtains high activity Phaeoporus obliquus powder.The preparation method of the high activity Phaeoporus obliquus powder of the present invention, can retain the biological activity of Phaeoporus obliquus to greatest extent, and crush efficiency when simultaneously pulverizing is high, and can prepare the Phaeoporus obliquus powder that particle diameter is less, improves the physico-chemical properties such as Phaeoporus obliquus powder such as adsorptivity.
Description
Technical field
The present invention relates to biomaterial extractive technique field, particularly relate to high activity Phaeoporus obliquus powder and preparation side thereof
Method.
Background technology
Phaeoporus obliquus, also known as Inonqqus obliquus (Inonotus obliquus), is that a kind of preciousness being born on silver birch is medicinal very
Bacterium, its sporophore is the warty form of carbonarius, appearance grey black, has an irregular groove mark, internal yellow, stockless, diameter 25～
40cm, dark, surface drastic crack, firmly, the most crisp time dry, part thickness 5mm can be educated, cot shape is thin, crineous.Its main component has many
Sugar, inonotus obliquus, Inonqqus obliquus alcohol, multiple oxidation triterpenoid compound, bolt bacterium lanosterol type sour, multiple triterpenes chemical combination
Thing, folic acid derivatives, aromatic vanillic acid, syringic acid and γ hydroxy benzoic acid, and tannin compound, steroid, biology
Alkaloid compound, melanin class, low molecule Polyphenols and lignin compound, can be effectively improved the vigor of immunocyte,
Anticancer diffusion and recurrence, prevent the absorption of the harmful substances such as carcinogen in gastrointestinal, and promote excretion.
The method preparing Phaeoporus obliquus product in prior art is that Phaeoporus obliquus is used water extraction mostly, concentrates, dries；Or it is straight
Tapping into after row is dried and pulverize, the finished particle after pulverizing is bigger.The equal biological activity of Phaeoporus obliquus product that said method prepares is relatively low,
As poor in physicochemical properties such as adsorptivities.
Summary of the invention
Based on this, it is necessary to provide the preparation method of a kind of high activity Phaeoporus obliquus powder.
The preparation method of a kind of high activity Phaeoporus obliquus powder, it is characterised in that comprise the steps:
Phaeoporus obliquus is placed in container, is filled with liquid inert gas, described Phaeoporus obliquus and liquid the most in the above-described container
The weight ratio of noble gas is 1:1～3；Standing cooling, the time is 10～30min；
Take out through the described Phaeoporus obliquus stood after cooling down, carry out vacuum sublimation dried；
Phaeoporus obliquus after described sublimation drying processes is carried out ball mill pulverizing, obtains high activity Phaeoporus obliquus powder.
In above-mentioned preparation method, described Phaeoporus obliquus is required to weight ratio and the standing of liquid inert gas cool time
Conservative control: the consumption of liquid inert gas very little, can cause liquid inert gas and Phaeoporus obliquus undercompounding, in Phaeoporus obliquus
The oxygen in portion etc. can not all be discharged, and causes its non-oxidizability to reduce；If consumption is too big, waste can be caused again, increase corresponding
Financial cost；And it is too short such as to stand time cool time, it is impossible to make the effective ingredient (polyphenols) of Phaeoporus obliquus with wooden
The contact of substrate weakens completely or separates, and is not easy to the precipitation of effective ingredient, and does not also reach Phaeoporus obliquus brittle temperature, enters
And reduce ball mill pulverizing efficiency；Long then production efficiency is low.
Wherein in an embodiment, described Phaeoporus obliquus is 1:1.5～2.5 with the weight ratio of described liquid inert gas；
The described time standing cooling is 15～25min.
Wherein in an embodiment, the temperature of described ball mill pulverizing is-100～-196 DEG C.
Wherein in an embodiment, the temperature of described ball mill pulverizing is-130～-170 DEG C.
Wherein in an embodiment, it is 1～8% through the water content of the dried Phaeoporus obliquus of described vacuum sublimation.Preferably
It is 3～5%.Further water content is carried out conservative control, it is to avoid the too high crushing effect that affects, reduce crush efficiency, or mistake
The low increase causing Financial cost.
Wherein in an embodiment, the technological parameter that described vacuum sublimation is dried is: vacuum 1.3～13 handkerchief, temperature-
10 DEG C～-50 DEG C.
Wherein in an embodiment, described liquid inert gas is liquid nitrogen or liquid helium.
Wherein in an embodiment, the particle diameter of described high activity Phaeoporus obliquus powder is 10～20 microns.
Wherein in an embodiment, described ball mill pulverizing is carried out in ball mill, and the steel ball that described ball mill uses is straight
Footpath is not more than 20mm, and rotating speed is not less than 100 turns/min.
The present invention also provides for the high activity Phaeoporus obliquus powder that the preparation method of described high activity Phaeoporus obliquus powder prepares
End.
Compared with prior art, the method have the advantages that
The preparation method of the high activity Phaeoporus obliquus powder of the present invention, uses liquid inert gas to cool down Phaeoporus obliquus,
And the consumption of conservative control liquid inert gas and cool time, make Phaeoporus obliquus while cooling, air therein can be by liquid
State noble gas is sufficiently displaced from, it is to avoid residual air causes raw material to aoxidize, and thus coordinates follow-up vacuum sublimation to be dried and ball milling
Pulverizing, can retain the biological activity of Phaeoporus obliquus to greatest extent, crush efficiency when simultaneously pulverizing is high, can prepare particle diameter less
Phaeoporus obliquus powder, improve the physico-chemical properties such as Phaeoporus obliquus powder such as adsorptivity, can be as functional food, health product, medicine
Raw material.
The preparation method technique of above-mentioned high activity Phaeoporus obliquus powder is simple, it is simple to commercial Application, the liquid simultaneously used is lazy
Property gas raw material is cheap and easily-available, it is possible to decrease production cost.
Detailed description of the invention
Below in conjunction with specific embodiment, high activity Phaeoporus obliquus powder of the present invention and preparation method thereof is made the most in detail
Explanation.
The present invention uses Phaeoporus obliquus to be fresh Phaeoporus obliquus raw material, thus avoids because early stage dry run is to product
The loss that matter and composition are caused.
Embodiment 1
The preparation method of the present embodiment a kind of high activity Phaeoporus obliquus powder, comprises the steps:
1, fresh Phaeoporus obliquus raw material is carried out preliminary treatment: be placed in suitable container by fresh Phaeoporus obliquus raw material,
Then being filled with liquid inert gas-nitrogen in container, Phaeoporus obliquus raw material is 1:2 with the weight ratio of liquid inert gas-nitrogen, stands
Cooling, the time is 20min；
2, taking out the Phaeoporus obliquus raw material after standing cooling, carry out vacuum sublimation dried, technological parameter is: vacuum 8
Handkerchief, temperature-30 DEG C, make the water content of Phaeoporus obliquus raw material 4%；
3, raw material dried to vacuum sublimation put into low temperature ball mill (temperature-150 DEG C, steel ball size be less than 20mm, turn
Speed is not less than 100 revs/min), being machined to particle diameter is 10～20 microns.
Embodiment 2
The preparation method of the present embodiment a kind of high activity Phaeoporus obliquus powder, comprises the steps:
1, fresh Phaeoporus obliquus raw material is carried out preliminary treatment: be placed in suitable container by fresh Phaeoporus obliquus raw material,
Then being filled with liquid inert gas-nitrogen in container, Phaeoporus obliquus raw material is 1:1 with the weight ratio of liquid inert gas-nitrogen, stands
Cooling, the time is 10min；
2, taking out the Phaeoporus obliquus raw material after standing cooling, carry out vacuum sublimation dried, technological parameter is: vacuum 13
Handkerchief, temperature-50 DEG C, make the water content of Phaeoporus obliquus raw material 1%；
3, raw material dried to vacuum sublimation put into low temperature ball mill (temperature-100 DEG C, steel ball size be less than 20mm, turn
Speed is not less than 100 revs/min), being machined to particle diameter is 10～20 microns.
Embodiment 3
The preparation method of the present embodiment a kind of high activity Phaeoporus obliquus powder, comprises the steps:
1, fresh Phaeoporus obliquus raw material is carried out preliminary treatment: be placed in suitable container by fresh Phaeoporus obliquus raw material,
Then being filled with liquid inert gas-nitrogen in container, Phaeoporus obliquus raw material is 1:3 with the weight ratio of liquid inert gas-nitrogen, stands
Cooling, the time is 30min；
2, taking out the Phaeoporus obliquus raw material after standing cooling, carry out vacuum sublimation dried, technological parameter is: vacuum
1.3 handkerchiefs, temperature-10 DEG C, make the water content of Phaeoporus obliquus raw material 8%；
3, raw material dried to vacuum sublimation put into low temperature ball mill (temperature-190 DEG C, steel ball size be less than 20mm, turn
Speed is not less than 100 revs/min), being machined to particle diameter is 10～20 microns.
Comparative example 1
The preparation method of this comparative example a kind of Phaeoporus obliquus powder, its step is with embodiment 1, and difference is: liquid inert gas
The quantity of body-nitrogen is 1:0.5, and standing cool time is 50min.
Comparative example 2
The preparation method of this comparative example a kind of Phaeoporus obliquus powder, its step is with embodiment 1, and difference is: liquid inert gas
The quantity of body-nitrogen is 1:5, and standing cool time is 5min.
Embodiment 1-3, comparative example 1-2 and traditional method (pulverize after directly drying and obtain Phaeoporus obliquus powder) are added
The Phaeoporus obliquus powder that work obtains carries out comparative testing below.
1, adsorptivity test, using activated carbon as comparison, result is as shown in table 1:
Table 1
Result of the test shows: the adsorptivity that the Phaeoporus obliquus powder that embodiment 1-3 prepares has had.
2, the clinical test of biological activity
Subjects is white mouse, is divided into 7 groups, often group 5: one of which is matched group, and remaining 6 groups is experimental group, takes
The powder of one of embodiment 1-3, comparative example 1-2 and traditional method.
In testing first 12 hours, subjects is not the most taken food, and the most often group pours into the riboflavin that concentration is 10mg/mL
Mononucleotide 2mL.After 30 minutes, experimental group pours into embodiment 1-3, comparative example 1-2 and the biography that 2mL content is 0.5%
The aqueous solution of the powder of one of system method.
The urine of white mouse in collecting one day, measures the flavin mononucleotide (FMN) content wherein remained, and result is as shown in table 2.
Table 2
Result of the test shows, the Phaeoporus obliquus powder that embodiment 1-3 obtains, the content of the residual in white mouse urine is more traditional
Method and comparative example 1-2 are low, illustrate that its effective biological activity and the drug absorption to intestinal are higher than traditional method and contrast
The powder that example 1-2 obtains；The industry such as pharmacy can be widely used in.
Each technical characteristic of embodiment described above can combine arbitrarily, for making description succinct, not to above-mentioned reality
The all possible combination of each technical characteristic executed in example is all described, but, as long as the combination of these technical characteristics is not deposited
In contradiction, all it is considered to be the scope that this specification is recorded.
Embodiment described above only have expressed the several embodiments of the present invention, and it describes more concrete and detailed, but also
Can not therefore be construed as limiting the scope of the patent.It should be pointed out that, come for those of ordinary skill in the art
Saying, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement, these broadly fall into the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. the preparation method of a high activity Phaeoporus obliquus powder, it is characterised in that comprise the steps:
Phaeoporus obliquus is placed in container, is filled with liquid inert gas, described Phaeoporus obliquus and liquid inert the most in the above-described container
The weight ratio of gas is 1:1～3；Standing cooling, the time is 10～30min；
Take out through the described Phaeoporus obliquus stood after cooling down, carry out vacuum sublimation and be dried；
Phaeoporus obliquus after described sublimation drying is carried out ball mill pulverizing, obtains high activity Phaeoporus obliquus powder.
The preparation method of high activity Phaeoporus obliquus powder the most according to claim 1, it is characterised in that described Phaeoporus obliquus and institute
State weight ratio 1:1.5～2.5 of liquid inert gas；The described time standing cooling is 15～25min.
The preparation method of high activity Phaeoporus obliquus powder the most according to claim 1, it is characterised in that described ball mill pulverizing
Temperature is-100～-196 DEG C.
The preparation method of high activity Phaeoporus obliquus powder the most according to claim 3, it is characterised in that described ball mill pulverizing
Temperature is-130～-170 DEG C.
The preparation method of high activity Phaeoporus obliquus powder the most according to claim 1, it is characterised in that through described vacuum sublimation
The water content of dried Phaeoporus obliquus is 1～8%.
The preparation method of high activity Phaeoporus obliquus powder the most according to claim 1, it is characterised in that described vacuum sublimation is done
Dry technological parameter is: vacuum 1.3～13 handkerchief, temperature-10 DEG C～-50 DEG C.
The preparation method of high activity Phaeoporus obliquus powder the most according to claim 1, it is characterised in that described liquid inert gas
Body is liquid nitrogen or liquid helium.
8. according to the preparation method of the high activity Phaeoporus obliquus powder described in any one of claim 1-7, it is characterised in that described height
The particle diameter of activity Phaeoporus obliquus powder is 10～20 microns.
9. according to the preparation method of the high activity Phaeoporus obliquus powder described in any one of claim 1-7, it is characterised in that described ball
Pulverizing is broken to be carried out in ball mill, and the steel ball size that described ball mill uses is not more than 20mm, and rotating speed is not less than 100 turns/min.
10. the high activity Betula platyphylla Suk. that the preparation method of the high activity Phaeoporus obliquus powder described in any one of claim 1-9 prepares
Fine and soft powder.
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| CN106137974B (en) | 2019-09-06 |
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