CN106136022A - A kind of preparation method of antiseptics for natural food - Google Patents
A kind of preparation method of antiseptics for natural food Download PDFInfo
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- CN106136022A CN106136022A CN201610477152.XA CN201610477152A CN106136022A CN 106136022 A CN106136022 A CN 106136022A CN 201610477152 A CN201610477152 A CN 201610477152A CN 106136022 A CN106136022 A CN 106136022A
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- grains
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
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- Food Science & Technology (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to the preparation method of a kind of antiseptics for natural food, belong to food antiseptic technical field.The present invention is directed to the existence of current chemical preservative and lure carcinous, teratogenesis shape and the problem easily causing alimentary toxicosis, first wine brewer's grains is processed by the present invention, as fermentation substrate, by cysteine hydrochloride, the microorganism in pickle juice is protected, separation and Extraction lactic acid bacteria therein, the probioticses such as actinomycetes, re-use screening culture medium wherein strain is screened, it is irradiated mutation again by β ray, strengthen survival strain and produce the ability of preservative substance, subsequently itself and fermentation substrate are carried out mixed fermentation, decompose wine brewer's grains, produce the polyphenols with antisepsis in glucose schlempe, subsequently by extracting, separate and be dried, it is prepared into antiseptics for natural food.
Description
Technical field
The present invention relates to the preparation method of a kind of antiseptics for natural food, belong to food antiseptic technical field.
Background technology
Adding preservative is that existing food extends the relatively conventional method of storage life, and food enterprise is when adding preservative one
As be all directly to add, do not carry out pretreatment.Because the preservative used in food is intended to meet " People's Republic of China's food
Product additive use sanitary standard " in regulation range and usage amount.These preservative have difference to different microorganisms
Inhibitory action, usual same preservative addition is the biggest, and antiseptic effect is the best.But, owing to numerous food product is in the course of processing
In will be through pyroprocess, and some preservative such as sorbic acid can volatilize, and this makes preservative amount retained few, and antiseptic effect is not
Good；There are again some food, such as fermented foods such as bread, need to add yeast in the course of processing, and the interpolation of preservative can press down
The growth of yeast processed, addition is the biggest, suppresses the most severe.
In order to extend the quality guarantee period of food, people use different means in food processing process, make microorganism live
Property lose, delay or stop it to grow.Add preservative to be one of which food antiseptic method easily and effectively and generally adopted
With.Commonly use in early days is chemical preservative, lures carcinous, teratogenecity and easily through some chemical preservative of discovery that studies for a long period of time
Cause the problems such as alimentary toxicosis, as benzoate may cause alimentary toxicosis phenomenon, nitrite and nitrate to give birth to
Become carcinogenic nitrosamine, nature ecological environment be will also result in adverse effect, the most strictly limit its use in food
Amount.The antiseptic effect of chemical preservative is also easily affected by factors such as dissolubility, food microorganisms kind, pH value.
Summary of the invention
The technical problem to be solved: exist for current chemical preservative and lure carcinous, teratogenesis shape and easily cause
The problem of alimentary toxicosis, first wine brewer's grains is processed by the present invention, as fermentation substrate, by cysteine hydrochloride pair
Microorganism in pickle juice is protected, the probiotics such as separation and Extraction lactic acid bacteria therein, actinomycetes, re-uses screening and culturing
Wherein strain is screened by base, then is irradiated mutation by β ray, strengthens survival strain and produces the ability of preservative substance,
Subsequently it is carried out with fermentation substrate mixed fermentation, decomposes wine brewer's grains, produce in glucose schlempe to have antisepsis many
Aldehydes matter, passes through extracting and developing subsequently and is dried, being prepared into antiseptics for natural food.
For solving above-mentioned technical problem, the present invention uses the technical scheme as described below to be:
(1) by solid-to-liquid ratio 1:2, take wine brewer's grains put into mass fraction be 10～15% sodium chloride solution in, at 30～35 DEG C
Under, stir 1～2h with 160r/min, add wine brewer's grains quality 1～the cellulase of 2%, using mass fraction is 30%
Hydrochloric acid solution regulation pH to 5.0～6.0, after stirring, at room temperature stand enzymolysis 2～5h, filter, use distillation
The flushed screening of water 3～4 times, obtain process wine brewer's grains, standby；
(2) by pickle juice and cysteine hydrochloride solution 2:3 by volume that mass fraction is 5%, mix homogeneously put into from
In scheming, under 2000r/min separate 3～5min, collect supernatant, count by weight, take 30～35 parts of MRS culture medium,
20～25 parts of wine brewer's grains, 3～6 parts of sodium nitrite, 15～20 parts of Carnis Bovis seu Bubali creams and 8～12 parts of skimmed milk powders, stir, and uses
Mass fraction is the phosphoric acid solution regulation pH to 4.5～5.0 of 30%, is placed in autoclave carrying out sterilizing, must screen training
Support base；
(3) by inoculum concentration 15～20%, the supernatant of above-mentioned gained is inoculated in the screening culture medium of above-mentioned gained, then will training
Foster base moves in shaking table incubator, carries out cultivating 10～12h, subsequently screening culture medium is placed in glass container at 28～32 DEG C
In, seal container, use nitrogen to be displaced by the air in container, at room temperature cultivate 4～5h, then use β roentgenization
Glass container 50～80s, continues to cultivate 1～2h；
(4) after above-mentioned cultivation terminates, screening culture medium is taken out, use the aseptic water washing screening and culturing of flow velocity 12mL/min
Primary surface, until media surface is without mycelia, collects flushing liquor, by solid-to-liquid ratio 1:2, by the process wine brewer's grains of step (1) gained
With flushing liquor mix homogeneously, being placed in fermentation tank, addition is the 30～40% of fermentation tank capacity, at room temperature with 120r/min
Stirring seals 1～3 day, filters fermenting mixture subsequently, collects filtrate, is taken off filtrate by activated carbon
Color, re-uses ultraviolet and the filtrate after decolouring is carried out sterilizing, be spray-dried subsequently, collect dried object,
Obtain antiseptics for natural food.
The application process of the present invention: by solid-to-liquid ratio 1:3～5, the antiseptics for natural food of gained of the present invention is mixed with water
Uniformly, uniformly it is sprayed on food surface or 1:10 in mass ratio, is added in food preparation process, within 2～3 days, detect, press down
Bacterium rate reaches 85～94%, and unable to take food product Acidity of Aikalinity affects, harmless, and easily decomposes in human body, as to human nutrition
Material is absorbed.
The present invention compared with additive method, Advantageous Effects:
(1) the preservative favorable anti-corrosion effect of gained of the present invention, Nantural non-toxic evil, to human body without any harmful effect；
(2) cost of manufacture of the present invention is low, it is easy to operation.
Detailed description of the invention
By solid-to-liquid ratio 1:2, take wine brewer's grains put into mass fraction be 10～15% sodium chloride solution in, at 30～35 DEG C
Under, stir 1～2h with 160r/min, add wine brewer's grains quality 1～the cellulase of 2%, using mass fraction is 30%
Hydrochloric acid solution regulation pH to 5.0～6.0, after stirring, at room temperature stand enzymolysis 2～5h, filter, use distillation
The flushed screening of water 3～4 times, obtain process wine brewer's grains, standby；By pickle juice and cysteine hydrochloric acid that mass fraction is 5%
Saline solution 2:3 by volume, mix homogeneously is put in centrifuge, separates 3～5min, collect supernatant, press under 2000r/min
Parts by weight meter, takes 30～35 parts of MRS culture medium, 20～25 parts of wine brewer's grains, 3～6 parts of sodium nitrite, 15～20 parts of Carnis Bovis seu Bubali creams
And 8～12 parts of skimmed milk powders, stir, the phosphoric acid solution regulation pH to 4.5～5.0 using mass fraction to be 30%, juxtaposition
In autoclave, carry out sterilizing, obtain screening culture medium；By inoculum concentration 15～20%, the supernatant of above-mentioned gained is inoculated in
In the screening culture medium of above-mentioned gained, then culture medium is moved in shaking table incubator, carry out cultivating at 28～32 DEG C 10～
12h, is placed in screening culture medium in glass container subsequently, seals container, uses nitrogen to be displaced by the air in container, in room
Temperature is lower cultivates 4～5h, then uses β roentgenization glass container 50～80s, continues to cultivate 1～2h；Terminate in above-mentioned cultivation
After, screening culture medium is taken out, uses the aseptic water washing screening and culturing primary surface of flow velocity 12mL/min until media surface
Without mycelia, collect flushing liquor, by solid-to-liquid ratio 1:2, by process wine brewer's grains and the flushing liquor mix homogeneously of gained, be placed in fermentation tank
In, addition is the 30～40% of fermentation tank capacity, at room temperature seals 1～3 day with 120r/min stirring, mixed to fermentation subsequently
Compound filters, and collects filtrate, is decoloured filtrate by activated carbon, re-uses ultraviolet to the filtration after decolouring
Liquid carries out sterilizing, is spray-dried subsequently, collects dried object, i.e. can get antiseptics for natural food.
Example 1
By solid-to-liquid ratio 1:2, take wine brewer's grains and put in the sodium chloride solution that mass fraction is 12%, at 32 DEG C, with 160r/
Min stirs 1.5h, adds the cellulase of wine brewer's grains quality 1.5%, and the hydrochloric acid solution using mass fraction to be 30% is adjusted
Joint pH to 5.0, after stirring, at room temperature stands enzymolysis 4h, filters, use distilled water flushing filtrate 4 times, obtain place
Reason wine brewer's grains, standby；By pickle juice and cysteine hydrochloride solution 2:3 by volume that mass fraction is 5%, mixing is all
Even put in centrifuge, under 2000r/min, separate 4min, collect supernatant, count by weight, take 34 parts of MRS culture medium,
23 parts of wine brewer's grains, 5 parts of sodium nitrite, 18 parts of Carnis Bovis seu Bubali creams and 10 parts of skimmed milk powders, stir, and using mass fraction is 30%
Phosphoric acid solution regulation pH to 4.5, be placed in autoclave carrying out sterilizing, obtain screening culture medium；By inoculum concentration 18%, will
The supernatant of above-mentioned gained is inoculated in the screening culture medium of above-mentioned gained, then culture medium is moved in shaking table incubator, 30
Carry out at DEG C cultivating 11h, subsequently screening culture medium is placed in glass container, seal container, use nitrogen by the sky in container
Gas displaces, and at room temperature cultivates 4h, then uses β roentgenization glass container 60s, continues to cultivate 1.5h；In above-mentioned cultivation
After end, screening culture medium is taken out, use the aseptic water washing screening and culturing primary surface of flow velocity 12mL/min until culture medium
Surface sterile silk, collects flushing liquor, by solid-to-liquid ratio 1:2, by process wine brewer's grains and the flushing liquor mix homogeneously of gained, is placed in and sends out
In ferment tank, addition is the 35% of fermentation tank capacity, at room temperature seals 2 days with 120r/min stirring, subsequently to fermentation mixing
Thing filters, and collects filtrate, is decoloured filtrate by activated carbon, re-uses ultraviolet to the filtrate after decolouring
Carry out sterilizing, be spray-dried subsequently, collect dried object, i.e. can get antiseptics for natural food.
By solid-to-liquid ratio 1:3, the antiseptics for natural food of gained of the present invention is mixed homogeneously with water, be uniformly sprayed on food table
Face or in mass ratio 1:10, be added in food preparation process, within 2 days, detects, and bacteriostasis rate reaches 85%, unable to take food product Acidity of Aikalinity
Impact, harmless, and easily decompose in human body, absorbed as to human nutrition material.
Example 2
By solid-to-liquid ratio 1:2, take wine brewer's grains and put in the sodium chloride solution that mass fraction is 15%, at 35 DEG C, with 160r/
Min stirs 2h, adds the cellulase of wine brewer's grains quality 2%, the hydrochloric acid solution regulation pH using mass fraction to be 30%
To 6.0, after stirring, at room temperature stand enzymolysis 5h, filter, use distilled water flushing filtrate 4 times, Portugal must be processed
Grape schlempe, standby；By pickle juice and cysteine hydrochloride solution 2:3 by volume that mass fraction is 5%, mix homogeneously is put
Enter in centrifuge, under 2000r/min separate 5min, collect supernatant, count by weight, take 35 parts of MRS culture medium, 20 parts
Wine brewer's grains, 3 parts of sodium nitrite, 15 parts of Carnis Bovis seu Bubali creams and 8 parts of skimmed milk powders, stir, and using mass fraction is the phosphorus of 30%
Acid solution regulation pH to 5.0, is placed in autoclave carrying out sterilizing, obtains screening culture medium；By inoculum concentration 20%, by above-mentioned
The supernatant of gained is inoculated in the screening culture medium of above-mentioned gained, then culture medium is moved in shaking table incubator, at 32 DEG C
Carry out cultivating 12h, subsequently screening culture medium is placed in glass container, seal container, use nitrogen to be put by the air in container
Swap out, at room temperature cultivate 5h, then use β roentgenization glass container 80s, continue to cultivate 2h；After above-mentioned cultivation terminates,
Screening culture medium is taken out, uses the aseptic water washing screening and culturing primary surface of flow velocity 12mL/min until media surface is aseptic
Silk, collects flushing liquor, by solid-to-liquid ratio 1:2, by process wine brewer's grains and the flushing liquor mix homogeneously of gained, is placed in fermentation tank,
Addition is the 40% of fermentation tank capacity, at room temperature seals 3 days with 120r/min stirring, carried out fermenting mixture subsequently
Filter, is collected filtrate, is decoloured filtrate by activated carbon, re-uses ultraviolet and sterilizes the filtrate after decolouring
Sterilization, is spray-dried subsequently, collects dried object, i.e. can get antiseptics for natural food.
By solid-to-liquid ratio 1:5, the antiseptics for natural food of gained of the present invention is mixed homogeneously with water, be uniformly sprayed on food table
Face or in mass ratio 1:10, be added in food preparation process, within 3 days, detects, and bacteriostasis rate reaches 94%, unable to take food product Acidity of Aikalinity
Impact, harmless, and easily decompose in human body, absorbed as to human nutrition material.
Example 3
By solid-to-liquid ratio 1:2, take wine brewer's grains and put in the sodium chloride solution that mass fraction is 10%, at 30 DEG C, with 160r/
Min stirs 1h, adds the cellulase of wine brewer's grains quality 1%, the hydrochloric acid solution regulation pH using mass fraction to be 30%
To 5.0, after stirring, at room temperature stand enzymolysis 2h, filter, use distilled water flushing filtrate 3 times, Portugal must be processed
Grape schlempe, standby；By pickle juice and cysteine hydrochloride solution 2:3 by volume that mass fraction is 5%, mix homogeneously is put
Enter in centrifuge, under 2000r/min separate 3min, collect supernatant, count by weight, take 30 parts of MRS culture medium, 25 parts
Wine brewer's grains, 6 parts of sodium nitrite, 20 parts of Carnis Bovis seu Bubali creams and 12 parts of skimmed milk powders, stir, and using mass fraction is the phosphorus of 30%
Acid solution regulation pH to 4.5, is placed in autoclave carrying out sterilizing, obtains screening culture medium；By inoculum concentration 15%, by above-mentioned
The supernatant of gained is inoculated in the screening culture medium of above-mentioned gained, then culture medium is moved in shaking table incubator, at 28 DEG C
Carry out cultivating 10h, subsequently screening culture medium is placed in glass container, seal container, use nitrogen to be put by the air in container
Swap out, at room temperature cultivate 4h, then use β roentgenization glass container 50s, continue to cultivate 1h；After above-mentioned cultivation terminates,
Screening culture medium is taken out, uses the aseptic water washing screening and culturing primary surface of flow velocity 12mL/min until media surface is aseptic
Silk, collects flushing liquor, by solid-to-liquid ratio 1:2, by process wine brewer's grains and the flushing liquor mix homogeneously of gained, is placed in fermentation tank,
Addition is the 30% of fermentation tank capacity, at room temperature seals 1 day with 120r/min stirring, carried out fermenting mixture subsequently
Filter, is collected filtrate, is decoloured filtrate by activated carbon, re-uses ultraviolet and sterilizes the filtrate after decolouring
Sterilization, is spray-dried subsequently, collects dried object, i.e. can get antiseptics for natural food.
By solid-to-liquid ratio 1:4, the antiseptics for natural food of gained of the present invention is mixed homogeneously with water, be uniformly sprayed on food table
Face or in mass ratio 1:10, be added in food preparation process, within 3 days, detects, and bacteriostasis rate reaches 92%, unable to take food product Acidity of Aikalinity
Impact, harmless, and easily decompose in human body, absorbed as to human nutrition material.
Claims (1)
1. the preparation method of an antiseptics for natural food, it is characterised in that concrete preparation process is:
(1) by solid-to-liquid ratio 1:2, take wine brewer's grains put into mass fraction be 10～15% sodium chloride solution in, at 30～35 DEG C
Under, stir 1～2h with 160r/min, add wine brewer's grains quality 1～the cellulase of 2%, using mass fraction is 30%
Hydrochloric acid solution regulation pH to 5.0～6.0, after stirring, at room temperature stand enzymolysis 2～5h, filter, use distillation
The flushed screening of water 3～4 times, obtain process wine brewer's grains, standby；
(2) by pickle juice and cysteine hydrochloride solution 2:3 by volume that mass fraction is 5%, mix homogeneously put into from
In scheming, under 2000r/min separate 3～5min, collect supernatant, count by weight, take 30～35 parts of MRS culture medium,
20～25 parts of wine brewer's grains, 3～6 parts of sodium nitrite, 15～20 parts of Carnis Bovis seu Bubali creams and 8～12 parts of skimmed milk powders, stir, and uses
Mass fraction is the phosphoric acid solution regulation pH to 4.5～5.0 of 30%, is placed in autoclave carrying out sterilizing, must screen training
Support base；
(3) by inoculum concentration 15～20%, the supernatant of above-mentioned gained is inoculated in the screening culture medium of above-mentioned gained, then will training
Foster base moves in shaking table incubator, carries out cultivating 10～12h, subsequently screening culture medium is placed in glass container at 28～32 DEG C
In, seal container, use nitrogen to be displaced by the air in container, at room temperature cultivate 4～5h, then use β roentgenization
Glass container 50～80s, continues to cultivate 1～2h；
(4) after above-mentioned cultivation terminates, screening culture medium is taken out, use the aseptic water washing screening and culturing of flow velocity 12mL/min
Primary surface, until media surface is without mycelia, collects flushing liquor, by solid-to-liquid ratio 1:2, by the process wine brewer's grains of step (1) gained
With flushing liquor mix homogeneously, being placed in fermentation tank, addition is the 30～40% of fermentation tank capacity, at room temperature with 120r/min
Stirring seals 1～3 day, filters fermenting mixture subsequently, collects filtrate, is taken off filtrate by activated carbon
Color, re-uses ultraviolet and the filtrate after decolouring is carried out sterilizing, be spray-dried subsequently, collect dried object,
Obtain antiseptics for natural food.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610477152.XA CN106136022A (en) | 2016-06-27 | 2016-06-27 | A kind of preparation method of antiseptics for natural food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610477152.XA CN106136022A (en) | 2016-06-27 | 2016-06-27 | A kind of preparation method of antiseptics for natural food |
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| CN106136022A true CN106136022A (en) | 2016-11-23 |
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| CN201610477152.XA Withdrawn CN106136022A (en) | 2016-06-27 | 2016-06-27 | A kind of preparation method of antiseptics for natural food |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689966A (en) * | 2016-12-25 | 2017-05-24 | 董晓 | Preparation method of natural food preservative |
| CN107518245A (en) * | 2017-09-08 | 2017-12-29 | 郭迎庆 | A kind of preparation method of food preservative |
-
2016
- 2016-06-27 CN CN201610477152.XA patent/CN106136022A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689966A (en) * | 2016-12-25 | 2017-05-24 | 董晓 | Preparation method of natural food preservative |
| CN107518245A (en) * | 2017-09-08 | 2017-12-29 | 郭迎庆 | A kind of preparation method of food preservative |
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Application publication date: 20161123 |
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