CN106135902A - A kind of diglyceride fluid composition rich in catalpic acid and its preparation method and application - Google Patents
A kind of diglyceride fluid composition rich in catalpic acid and its preparation method and application Download PDFInfo
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Abstract
The present invention relates to a kind of diglyceride fluid composition rich in catalpic acid, this diglyceride fluid composition is made up of following components by mass percentage: 50～the monoglyceride of the triglyceride of the diglyceride of 90%, 9～45%, 0.1～10% and 0.02～the residue of 10%, in the whole glyceride being made up of above-mentioned monoglyceride, diglyceride and triglyceride, catalpic acid is 0.2～0.8:1 with the mass ratio of fatty acid, and described residue is free fatty and glycerol；Further relate to the preparation method of said composition and controlling body weight, the application improved in blood fat and the functional food of raising immunity and medicine.The diglyceride fluid composition rich in catalpic acid in the present invention had both had the effect of weight reducing of diglyceride, also there is the effects such as the antiinflammatory of catalpic acid, fat-reducing simultaneously, so that said composition can be widely used in control body weight, improve in blood fat and the functional food of raising immunity and medicine, in a word, the preparation technology of said composition is simple, low cost, has broad application prospects.
Description
Technical field
The present invention relates to applied chemistry technical field, particularly relate to a kind of diglyceride fluid composition rich in catalpic acid and its preparation method and application.
Background technology
Diglyceride (Diacylglycerol) is the structured lipid that in a quasi-glycerol three-ester (Triacylglycerol), a fatty acid is optionally substituted by a hydroxyl group, it is micro constitutent and the endogenous centre of body fat metabolism of natural plant grease, it has two kinds of natural isomerss, i.e. 1,2-diglyceride and 1,3-diglyceride, as shown in Equation 1.
Formula 1 diglyceride structural representation (wherein COR, COR ' be saturated or unsaturated fatty acyl group)
7 kinds of article such as at present, finding through multinomial safety evaluation, diglyceride is harmless to human non-toxic, is regarded as " It is generally accepted safety " material by U.S. FDA, No. 18 bulletin approval triglyceride oil of ministry of Health of China in 2009 are new resource food.Although diglyceride and triglyceride can be worth and on absorbance essentially identical, but there is larger difference in they metabolism in vivo, short-term and long-term animal and human trial show, compared with the triglyceride formed containing same or similar fatty acid, 1,3-DAG has control and treats high blood triglyceride disease, obesity and the effect of complication thereof.Therefore, the edible oil rich in 1,3-DAG is possible not only to the oil for health care substituting triglyceride edible oil as healthy population, it is also possible to for obese people and the nutrition treatment of related complication crowd.
At present, the preparation method of diglyceride mainly has following several: (1) triglyceride alcoholysis method: i.e. under the catalysis of lipase, triglyceride reacts with lower alcohol, generate diglyceride and low-carbon-ester, but lipase the Novozyme 435 and Lipozyme IM that the method uses can only specific cleavage or synthesis sn-1 position or the ester bond of sn-3 position, therefore can only obtain 1,2-diglyceride.(2) partial hydrolysis, esterification process: i.e. first partial hydrolysis triglyceride obtains fatty acid, add glycerol, under the catalysis of sn-1 and sn-3 position selected fat enzyme, the fatty acid generation esterification that glycerol and partial hydrolysis obtain generates 1, 3-diglyceride, current most diglyceride includes that the EnovaTM Oil that KAO. Corp. SA produces develops based on this principle, the shortcoming of the method maximum is that technological process is complicated, multistep is needed to separate, in addition the utilization rate of raw material is low, additionally need use lipase, and lipase price is higher, there is the problems such as high cost for industrial scale produces diglyceride.(3) glycerol solution: will triglyceride oil and glycerol and a kind of mix containing monocarboxylic acid or the alkali metal salt of dicarboxylic acids or alkali salt or its mixture (making catalyst), by the glycerolysis of triglyceride, prepare business, food stage 1,3-triglyceride oil, but conventional glycerol rhizolomy needs to react under the high temperature of 220～260 DEG C so that the Oxidation of triglyceride, diglyceride and fatty acid particularly unsaturated fatty acid strengthens.At present, prepare triglyceride oil to be mainly by triglyceride type oil and fat preparations such as soybean oil, Oleum Brassicae campestris, Oleum Arachidis hypogaeae semen and Semen Maydis oil, prepare diglyceride with fish oil etc. for raw material and the most also have a small amount of report, as (2010) such as Feltes prepare the diglyceride rich in n-3PUFA with lipase Novozyme 435 by triglyceride alcoholysis method；Eoma etc. (2010) synthesize the diglyceride rich in n-3PUFA with lipase RMIM and Novozyme 435 by hydrolysis, esterification process.
Conjugated fatty acid is the fatty acid that a class contains two or more conjugate double bond system, modal conjugated fatty acid is octadecadienoic acid and jeceric acid, i.e. conjugated linoleic acid (CLA) and conjugate linolenic acid (CLNA), wherein CLA is linoleic conjugate form, and CLNA is the conjugate form of alpha-linolenic acid.CLA with CLNA has multiple different isomer because the position of double bond is different with configuration, 9 is suitable, 11 trans-conjugated linoleic acid (cis9, tras11-18:2), 10 is anti-, 12 cis-conjugated linoleic acid (tras10, and punicic acid (cis 9 cis12-18:2), tras11, cis13-18:3), 2-ketoacid (c9, tras11, tras 13-18:3), catalpic acid (tras9, tras11, cis13-18:3) respectively be study most commonly used CLA and CLNA isomer.
Catalpic acid (Catalpic acid, trans9, trans11, cis13-18:3) it is the important conjugate linolenic acid of one being naturally occurring in Catalpa ovata Don. seed, catalpic acid is 9,11,13 double bonds conjugation, 9 and 11 double bonds are configured as trans, and 13 double bonds are configured as cis conjugate linolenic acid (trans9, trans11, cis13-CLNA).Catalpic acid and cis9, the similarity of trans11-CLA (9,11 double bond conjugation) is that their fatty acid chain the 9th and the 11st is all undersaturated double bond.Cis9, trans11-CLA and trans10, cis12-CLA is the topmost two kinds of isomers of CLA, and naturally occurring CLA is mainly cis9, trans11-CLA, accounts for CLA more than 90%, is also the activity form of the CLA being most widely used in American-European countries at present.
Catalpic acid and cis9, the crucial difference of trans11-CLA is the 3rd undersaturated double bond of catalpic acid, and catalpic acid has a trans double bond the 13rd of fatty acid chain, so, catalpic acid has the feature of " polyunsaturated fatty acid ".Polyunsaturated fatty acid has important nutritive value, and triglyceride and cholesterol in humans and animals body are had useful effect, can reduce the danger of cardiovascular and cerebrovascular disease, promotes g and D.At present, polyunsaturated fatty acid is widely used in the development adding growth promoter and the intelligence promoting baby in baby food to.
Conjugated fatty acid has the special physiological function different from conventional polyunsaturated fatty acid.It is reported, CLNA has anticancer, atherosclerosis, improve blood fat, improve immunity, reduce body fat content, improve lean body mass and improve the most favourable physiological function such as insulin resistant, such as United States Patent (USP) NO7,834,058 disclose utilize catalpic acid treat hyperinsulinemia, United States Patent (USP) NO7,687,544 disclose and utilize catalpic acid treatment type 2 diabetes mellitus and its related syndrome.On the other hand, CLA is in the whole world, and especially American-European countries is used widely as the functional food such as fat-reducing, blood fat reducing and health product.Research finds that CLA is naturally occurring in ruminant food article such as beef, Carnis caprae seu ovis and milk product thereof, but CLA content in these foods only has about 1% (Sehat etc., 1998) so that can not be used as containing food CLA, sanatory or the source of medicine with naturally occurring CLA.Therefore the CLA of commercial is mainly by obtaining rich in the further isomerization of linoleic oils and fats, this isomerization product generally comprises equimolar cis9, trans11-CLA and trans10, cis12-CLA (Pariza etc., 2001) and other isomers.Not only there is the consideration of the aspects such as safety in consumer to the CLA of preparation of industrialization, and the industrialization of CLA itself there is also a lot of problem, such as: CLA industrialized production Costco Wholesale is higher；The CLA of preparation of industrialization has various isomeric forms, and is not that every kind of isomer all has the function useful to human body, wherein cis9, and trans11-CLA is the active isomer that safety is higher.
Summary of the invention
First to be solved by this invention technical problem is that and provide a kind of diglyceride fluid composition rich in catalpic acid for prior art.
Second to be solved by this invention technical problem is that the preparation method providing a kind of above-mentioned diglyceride fluid composition rich in catalpic acid for prior art.
3rd to be solved by this invention technical problem is that the application providing a kind of above-mentioned diglyceride fluid composition rich in catalpic acid for prior art.
The present invention solves the technical scheme that first technical problem used: a kind of diglyceride fluid composition rich in catalpic acid, it is characterized in that, this diglyceride fluid composition is made up of following components by mass percentage: 50～the monoglyceride of the triglyceride of the diglyceride of 90%, 9～45%, 0.1～10% and 0.02～the residue of 10%, in the whole glyceride being made up of above-mentioned monoglyceride, diglyceride and triglyceride, catalpic acid is 0.2～0.8:1 with the mass ratio of fatty acid, and described residue is free fatty and glycerol.
The present invention solves the technical scheme that second technical problem used: the preparation method rich in the diglyceride fluid composition of catalpic acid in such scheme comprises the following steps:
The first step, the extraction of catalpic acid:
(1) pretreatment: Catalpa ovata Don. seed is cleaned, drained, is dried at 40～60 DEG C；
(2) catalpic acid triglyceride extracts: dried Catalpa ovata Don. seed obtains dry powder after pulverizing, dry powder is through solvent extraction supercritical extraction or directly colds pressing and squeezes to obtain catalpic acid triglyceride crude extract, and the refine after degumming, deacidification, decolouring, deodorize, dewaxing of catalpic acid triglyceride crude extract prepares catalpic acid triglyceride；
(3) preparation of catalpic acid: the catalpic acid triglyceride prepared in described step (2) is obtained catalpic acid after saponification, acidifying；
(4) purification: the catalpic acid and the Catalpa ovata Don. acid esters that step (3) are obtained are dissolved in organic solvent, is respectively the catalpic acid of 95～98% by two step freeze crystallization isolated purity；
Second step, the catalpic acid of said extracted and glycerol are mixed to form mixture with the ratio of 2～10:1, add lipozyme RMIM in the mixture and carry out the prepared diglyceride fluid composition crude product rich in catalpic acid of enzymic catalytic reaction under vacuo, reaction temperature is 30～60 DEG C, response time 8-12h, described mixture is 100～500:1 with the mass ratio of lipozyme RMIM；
3rd step, to the above-mentioned prepared diglyceride fluid composition crude product rich in catalpic acid by the acid of molecular distillation fractionation of fatty and monoglyceride, uses the method for decolouring, deodorize and filtration to refine the diglyceride fluid composition rich in catalpic acid needed for acquisition the most again.
As preferably, the extractant in described step (2) is one or more mixture in normal hexane, petroleum ether, ether, ethanol, methanol, chloroform, n-butyl alcohol, acetone, hexamethylene.
As preferably, described two-step crystallization method includes that first step crystallization and second step crystallize, and the crystallization temperature of this first step crystallization is 4 DEG C, and crystallization time is 8～16h, and crystallization pressure is normal pressure；The crystallization temperature of second step crystallization is-20 DEG C～-70 DEG C, and crystallisation times is 3-5 time, and each crystallization time is 8～16h, and crystallization pressure is normal pressure.
The present invention solves the 3rd technical scheme that technical problem is used: should be rich in the application in functional food and medicine of the diglyceride fluid composition of catalpic acid, and described functional food and medicine are at least for controlling body weight, improving blood fat or the wherein one side improving in immunity.
Compared with prior art, it is an advantage of the current invention that: the diglyceride fluid composition rich in catalpic acid in the present invention combines with catalpic acid with triglyceride form, compared with conventional diglyceride product, the preventive effect to fat generation can be strengthened, thus reach to control the purpose of body weight, and said composition also has various nutritional labelings and the physiological advantages of catalpic acid in addition to having the structural stability of oil, hence in so that the product prepared with said composition for active component more superiority compared with conventional products, the most both there is the effect of weight reducing of diglyceride, also there is the antiinflammatory of catalpic acid simultaneously, the effects such as fat-reducing, so that said composition can be widely used in has control body weight, improve in functional food and the medicine of the effects such as blood fat and raising immunity.Meanwhile, in the preparation process of said composition, prepare catalpic acid, Catalpa ovata Don. seed abundance with Catalpa ovata Don. seed for raw material, edible safety is high, and the catalpic acid high purity 95～98% prepared, in a word, the preparation technology of said composition is simple, and low cost has broad application prospects.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
Collecting Catalpa ovata Don. seed, pulverize to obtain dry powder 1000g after 40 DEG C of oven for drying, obtain the triglyceride crude extract of green with the petroleum ether of 5 times of volumes at less than 60 DEG C after reflux, extract, 6h, oil yield is 28.2%.Take triglyceride crude extract 50mL and add the potassium hydroxide-ethanol solution 200mL of 2M 90% saponification 2h at nitrogen protects lower 60 DEG C; add three extractings of 50mL petroleum ether after adding 50mL water and remove not saponification compound; add in 6M hydrochloric acid 100mL and go to upper strata to obtain petroleum ether solution with petroleum ether extracting after acidifying, then after rotary evaporation in vacuo, obtaining catalpic acid crude extract.Catalpic acid crude extract dissolves in ethanol, and the lower 4 DEG C of crystallization 8h of normal pressure remove satisfied fatty acid, and the ethanol solution obtained crystallizes 16h in subzero 20 DEG C the most at ambient pressure, is repeated 3 times the highly purified catalpic acid of freezing and crystallizing isolated.The catalpic acid using above-mentioned technique to prepare in the present embodiment reaches 97% through gas chromatographic analysis purity.
The catalpic acid of said extracted and glycerol are mixed to form mixture with the ratio of 2:1, add lipozyme RMIM (Novozymes Company of Denmark) in the mixture and carry out the prepared diglyceride fluid composition crude product rich in catalpic acid of enzymic catalytic reaction under vacuo, reaction temperature is 30 DEG C, response time 12h, said mixture is 100:1 with the mass ratio of lipozyme RMIM.To the above-mentioned prepared diglyceride fluid composition crude product rich in catalpic acid by the acid of molecular distillation fractionation of fatty and monoglyceride, the method for decolouring, deodorize and filtration is used to refine the diglyceride fluid composition rich in catalpic acid needed for acquisition the most again.
After testing, diglyceride fluid composition rich in catalpic acid prepared by the present embodiment is made up of following components in parts by weight: the diglyceride of 50%, the triglyceride of 30%, the monoglyceride of 10% and the residue of 10%, in the whole glyceride being made up of above-mentioned monoglyceride, diglyceride and triglyceride, catalpic acid is 0.2:1 with the mass ratio of fatty acid, and above-mentioned residue is free fatty and glycerol.
Embodiment 2:
Collect Catalpa ovata Don. seed, the dry powder 1000g of particle diameter 0.4～0.5mm is pulverized to obtain after natural air drying under sunlight, at 30MPa, use SCF-CO 2 (the HA221-50-06 type supercritical extraction unit that Nantong city Huaan supercritical extraction unit produces) 2h under the conditions of 40 DEG C, obtaining the triglyceride crude extract (crude oil) of green at separating pressure 15MPa, separation temperature 50 DEG C, oil yield is 30.2%；Crude oil refine after degumming, deacidification, decolouring, deodorize, dewaxing prepares triglyceride.Take triglyceride crude extract 50mL and add the potassium hydroxide-ethanol solution 200mL of 2M 90% saponification 2h at nitrogen protects lower 60 DEG C; add three extractings of 50mL petroleum ether after adding 50mL water and remove not saponification compound; add in 6M hydrochloric acid 100mL and go to upper strata to obtain petroleum ether solution with petroleum ether extracting after acidifying, then after rotary evaporation in vacuo, obtaining catalpic acid crude extract.Catalpic acid crude extract dissolves in acetone, and the lower 4 DEG C of crystallization 8h of normal pressure remove satisfied fatty acid, then crystallize 8h in subzero 45 DEG C at ambient pressure, repeat more than 5 the highly purified catalpic acid of freezing and crystallizing isolated.The catalpic acid using above-mentioned technique to prepare in the present embodiment reaches 95% through gas chromatographic analysis purity.
The catalpic acid of said extracted and glycerol are mixed to form mixture with the ratio of 10:1, add lipozyme RMIM (Novozymes Company of Denmark) in the mixture and carry out the prepared diglyceride fluid composition crude product rich in catalpic acid of enzymic catalytic reaction under vacuo, reaction temperature is 60 DEG C, response time 8h, said mixture is 500:1 with the mass ratio of lipozyme RMIM.To the above-mentioned prepared diglyceride fluid composition crude product rich in catalpic acid by the acid of molecular distillation fractionation of fatty and monoglyceride, the method for decolouring, deodorize and filtration is used to refine the diglyceride fluid composition rich in catalpic acid needed for acquisition the most again.
After testing, diglyceride fluid composition rich in catalpic acid prepared by the present embodiment is made up of following components in parts by weight: the diglyceride of 70%, the triglyceride of 19%, the monoglyceride of 5% and the residue of 6%, in the whole glyceride being made up of above-mentioned monoglyceride, diglyceride and triglyceride, catalpic acid is 0.8:1 with the mass ratio of fatty acid, and above-mentioned residue is free fatty and glycerol.
Embodiment 3:
Collecting Catalpa ovata Don. seed, pulverize to obtain dry powder 1000g after 40 DEG C of oven for drying, obtain green catalpic acid triglyceride crude extract after at room temperature extract 24h with the chloroform of 5 times of volumes and methanol mixed solution (volume ratio is as 1:1), oil yield is 29.7%.Take triglyceride crude extract 100mL and add the sodium hydroxide ethanol solution 400mL of 2M 90% saponification 2h at nitrogen protects lower 60 DEG C; add three extractings of 100mL petroleum ether after adding 1000mL water and remove not saponification compound; add in 6M hydrochloric acid 200mL and go to upper strata to obtain petroleum ether solution with petroleum ether extracting after acidifying; catalpic acid crude extract 4 DEG C of crystallization 10h at ambient pressure remove satisfied fatty acid; crystallize 12h in subzero 40 DEG C the most at ambient pressure, be repeated 4 times the highly purified catalpic acid of freezing and crystallizing isolated.The catalpic acid using above-mentioned technique to prepare in the present embodiment reaches 96% through gas chromatographic analysis purity.
The catalpic acid of said extracted and glycerol are mixed to form mixture with the ratio of 6:1, add lipozyme RMIM (Novozymes Company of Denmark) in the mixture and carry out the prepared diglyceride fluid composition crude product rich in catalpic acid of enzymic catalytic reaction under vacuo, reaction temperature is 45 DEG C, response time 12h, said mixture is 300:1 with the mass ratio of lipozyme RMIM.To the above-mentioned prepared diglyceride fluid composition crude product rich in catalpic acid by the acid of molecular distillation fractionation of fatty and monoglyceride, the method for decolouring, deodorize and filtration is used to refine the diglyceride fluid composition rich in catalpic acid needed for acquisition the most again.
After testing, diglyceride fluid composition rich in catalpic acid prepared by the present embodiment is made up of following components in parts by weight: the diglyceride of 90%, the triglyceride of 9%, the monoglyceride of 0.1% and the residue of 0.9%, in the whole glyceride being made up of above-mentioned monoglyceride, diglyceride and triglyceride, catalpic acid is 0.5:1 with the mass ratio of fatty acid, and above-mentioned residue is free fatty and glycerol.
Embodiment 4:
Collecting Catalpa ovata Don. seed, pulverize to obtain the dry powder 1000g of particle diameter 0.4～0.5mm, obtain catalpic acid triglyceride crude extract with the petroleum ether of 5 times of volumes at less than 80 DEG C after reflux, extract, 6h under sunlight after natural air drying, oil yield is 28.8%.The refine after degumming, deacidification, decolouring, deodorize, dewaxing of catalpic acid triglyceride crude extract prepares triglyceride.Take triglyceride crude extract 50mL and add the potassium hydroxide-ethanol solution 200mL of 2M 90% saponification 2h at nitrogen protects lower 60 DEG C; add three extractings of 50mL petroleum ether after adding 50mL water and remove not saponification compound; add in 6M hydrochloric acid 100mL and go to upper strata to obtain petroleum ether solution with petroleum ether extracting after acidifying, then after rotary evaporation in vacuo, obtaining catalpic acid crude extract.Catalpic acid crude extract dissolves in acetone, and the lower 4 DEG C of crystallization 8h of normal pressure remove satisfied fatty acid, then crystallize 8h in subzero 45 DEG C at ambient pressure, repeat more than 5 the highly purified catalpic acid of freezing and crystallizing isolated.The catalpic acid using above-mentioned technique to prepare in the present embodiment reaches 95% through gas chromatographic analysis purity.
The catalpic acid of said extracted and glycerol are mixed to form mixture with the ratio of 4:1, add lipozyme RMIM (Novozymes Company of Denmark) in the mixture and carry out the prepared diglyceride fluid composition crude product rich in catalpic acid of enzymic catalytic reaction under vacuo, reaction temperature is 40 DEG C, response time 10h, said mixture is 200:1 with the mass ratio of lipozyme RMIM.To the above-mentioned prepared diglyceride fluid composition crude product rich in catalpic acid by the acid of molecular distillation fractionation of fatty and monoglyceride, the method for decolouring, deodorize and filtration is used to refine the diglyceride fluid composition rich in catalpic acid needed for acquisition the most again.
After testing, diglyceride fluid composition rich in catalpic acid prepared by the present embodiment is made up of following components in parts by weight: the diglyceride of 53.98%, the triglyceride of 45%, the monoglyceride of 1% and the residue of 0.02%, in the whole glyceride being made up of above-mentioned monoglyceride, diglyceride and triglyceride, catalpic acid is 0.4:1 with the mass ratio of fatty acid, and above-mentioned residue is free fatty and glycerol.
Embodiment 5:
5.1, rich in control action to body weight of the diglyceride fluid composition of catalpic acid (Catalpic acid)
Appointing to take and carried out zoopery by the diglyceride fluid composition rich in catalpic acid prepared in above-described embodiment 1～4, research said composition is the most inhibited to the increase of body weight and body fat.
The male ICR mouse of 6 week old is provided by Zhejiang Province's Experimental Animal Center, and ICR mice is randomly divided into 2 groups after adapting to 7 days, often group 10.In following 6 week, 2 groups of mices are in addition to feeding feedstuff, respectively to give each mice rich in the diglyceride fluid composition of catalpic acid and per os gavage of consumption of catalpic acid triglyceride oil (catalpic acid content is for 42%) 50mg/kg body weight every day, measuring the body weight of each mice every two weeks, the meansigma methods of surveyed body weight is as shown in table 1.As it can be seen from table 1 compared with catalpic acid triglyceride line of oils, Mouse Weight can be significantly inhibited rich in the diglyceride fluid composition of catalpic acid and gather way, control the growth of body weight.
Table 1 is rich in the impact on Mouse Weight of the diglyceride fluid composition of catalpic acid
5.2, the impact on blood fat
Appointing to take and carried out zoopery by the diglyceride fluid composition rich in catalpic acid prepared in above-described embodiment 1～4, whether research said composition has improvement result to blood fat.
The male ICR mouse of 6 week old is provided by Zhejiang Province's Experimental Animal Center, and ICR mice is randomly divided into 2 groups after adapting to 7 days, often group 10.In following 6 week, 2 groups of mices are in addition to feeding feedstuff, respectively to give each mice rich in the diglyceride fluid composition of catalpic acid and per os gavage of consumption of catalpic acid triglyceride oil (catalpic acid content is for 42%) 50mg/kg body weight every day, experiment takes snow and measures lipid of mice after terminating.The meansigma methods of surveyed blood fat is as shown in table 2.As can be seen from Table 2, compared with catalpic acid triglyceride line of oils, diglyceride fluid composition rich in catalpic acid can significantly improve mice HDL-C content (HDL-C), reduce low-density lipoprotein cholesterol (LDL-C) content, improving the ratio of LDL-C Yu HDL-C, this shows to significantly improve lipid of mice rich in the diglyceride fluid composition of catalpic acid.
Table 2 is rich in the impact on lipid of mice of the diglyceride fluid composition of catalpic acid
Claims (5)
1. the diglyceride fluid composition rich in catalpic acid, it is characterised in that this diglyceride fluid composition presses matter
Amount percent meter is made up of following components: 50～the glycerol of the triglyceride of the diglyceride of 90%, 9～45%, 0.1～10%
One ester and 0.02～the residue of 10%, the whole glyceride being made up of above-mentioned monoglyceride, diglyceride and triglyceride
Middle catalpic acid is 0.2～0.8:1 with the mass ratio of fatty acid, and described residue is free fatty and glycerol.
2. a preparation method for the diglyceride fluid composition rich in catalpic acid as claimed in claim 1, its feature
It is to comprise the following steps: the first step, the extraction of catalpic acid:
(1) pretreatment: Catalpa ovata Don. seed is cleaned, drained, is dried at 40～60 DEG C；
(2) catalpic acid triglyceride extracts: dried Catalpa ovata Don. seed pulverize after dry powder, dry powder is through solvent extraction, super
Critical extraction or directly cold pressing and squeeze to obtain catalpic acid triglyceride crude extract, catalpic acid triglyceride crude extract is through degumming, de-
After acid, decolouring, deodorize, dewaxing, refine prepares catalpic acid triglyceride；
(3) preparation of catalpic acid: the catalpic acid triglyceride prepared in described step (2) is obtained after saponification, acidifying
To catalpic acid；
(4) purification: the catalpic acid and the Catalpa ovata Don. acid esters that step (3) are obtained are dissolved in organic solvent, freezing by two steps
Crystallization process isolated purity is respectively the catalpic acid of 95～98%；
Second step, is mixed to form mixture with the ratio of 2～10:1, in this mixing by the catalpic acid of said extracted and glycerol
Thing adds lipozyme RMIM and carries out the prepared triglyceride oil combination rich in catalpic acid of enzymic catalytic reaction under vacuo
Thing crude product, reaction temperature is 30～60 DEG C, response time 8-12h, described mixture and the quality of lipozyme RMIM
Ratio is 100～500:1；
3rd step, to the above-mentioned prepared diglyceride fluid composition crude product rich in catalpic acid by molecular distillation fractionation of fatty
Acid and monoglyceride, use the method for decolouring, deodorize and filtration to refine the glycerol rich in catalpic acid needed for acquisition the most again
Diester oil compositions.
3. the preparation method of the diglyceride fluid composition rich in catalpic acid as claimed in claim 2, it is characterised in that
Extractant in described step (2) be normal hexane, petroleum ether, ether, ethanol, methanol, chloroform, n-butyl alcohol,
One or more mixture in acetone, hexamethylene.
4. the preparation method of the diglyceride fluid composition rich in catalpic acid as claimed in claim 2, it is characterised in that
Described two-step crystallization method includes that first step crystallization and second step crystallize, and the crystallization temperature of this first step crystallization is 4 DEG C, crystallization
Time is 8～16h, and crystallization pressure is normal pressure；The crystallization temperature of second step crystallization is-20 DEG C～-70 DEG C, and crystallisation times is 3-5
Secondary, each crystallization time is 8～16h, and crystallization pressure is normal pressure.
5. the diglyceride fluid composition rich in catalpic acid as claimed in claim 1 is in functional food and medicine
Application, described functional food and medicine are at least for controlling body weight, improving blood fat or improve the wherein side in immunity
Face.
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| CN108477309A (en) * | 2018-02-26 | 2018-09-04 | 浙江海洋大学 | A kind of phosphatide type catalpic acid fat or oil composition and its preparation method and application |
| CN116077422A (en) * | 2023-01-04 | 2023-05-09 | 成都大学 | Pomegranate seed oil diglyceride nanoemulsion gel |
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| CN116077422A (en) * | 2023-01-04 | 2023-05-09 | 成都大学 | Pomegranate seed oil diglyceride nanoemulsion gel |
| CN116077422B (en) * | 2023-01-04 | 2024-02-13 | 成都大学 | Pomegranate seed oil diglyceride nanoemulsion gel |
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