CN106124776A - CA724 chemiluminescence immune detection reagent kit and preparation method thereof - Google Patents
CA724 chemiluminescence immune detection reagent kit and preparation method thereof Download PDFInfo
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- CN106124776A CN106124776A CN201610514452.0A CN201610514452A CN106124776A CN 106124776 A CN106124776 A CN 106124776A CN 201610514452 A CN201610514452 A CN 201610514452A CN 106124776 A CN106124776 A CN 106124776A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/70—Mechanisms involved in disease identification
- G01N2800/7023—(Hyper)proliferation
- G01N2800/7028—Cancer
Abstract
The invention discloses a kind of CA724 chemiluminescence immune detection reagent kit and preparation method thereof, CA724 chemiluminescence immune detection reagent kit includes: the coated carboxylated magnetic particle of CA724 monoclonal antibody and the CA724 monoclonal antibody of chemiluminescent labels labelling.This CA724 chemiluminescence immune detection reagent kit can complete the detection of CA724 with Full-automatic chemiluminescence immunoassay analysis meter for detection instrument.This CA724 chemiluminescence immune detection reagent kit, through experiment, its detection sensitivity reaches 0.2U/mL, at least improves 10 times relative to the detection method sensitivity of traditional CA724, and the accuracy of detection of this CA724 chemiluminescence immune detection reagent kit is higher.
Description
Technical field
The present invention relates to vitro detection field, particularly relate to a kind of CA724 chemiluminescence immune detection reagent kit
And preparation method thereof.
Background technology
Tumor has constituted serious threat to human survival, and health and quality of life to the mankind cause huge damage.
Treatment tumor it is critical only that in time discovery, and check after early treatment and treatment.Sugar antigen is the most widely used
One class tumor markers, it includes glycoprotein and lipolysaccharide, often shows as mucin form.Its antigenic determinant can be positioned on sugar chain
Go up or on pyrenoids.Difference according to glycosyl epi-position and the difference of epitope size, generally can be divided into CA50, CA125,
CA153, CA199, CA242, CA724, human body original sugar antigen content is unusual trace, even without, but when pernicious
Tumor may induce the sugar antigen of body secretes high concentration when occurring, therefore sugar antigen can be tumor early stage examination,
Clinical diagnosis, curative effect monitoring, cancer cell metastasis and postoperative check provide data foundation.
The common methods of Clinical detection CA724 (CA724) has enzyme linked immunosorbent assay, radioimmunoassay, RIA at present
Method, enzyme-catalyzed chemical luminescence method, but in place of these methods all also exist some shortcomings.
The detection method of traditional CA724 (CA724) mainly uses enzyme linked immunosorbent assay, but, it is limited to
The feature of enzyme linked immunological, the detection method of traditional CA724 is in open space during detection, easily causes
Cross-contamination between various reagent, and use manual operations owing to detection process, the dosage of reagent or sample is the most smart
Really, operating process is the most loaded down with trivial details and complicated, is susceptible to bust, and accuracy of detection is poor.
Summary of the invention
Based on this, it is necessary to provide the CA724 chemiluminescence immunoassay detectable that a kind of detection sensitivity is higher
Box and preparation method thereof.
A kind of CA724 chemiluminescence immune detection reagent kit, including: CA724 monoclonal antibody is coated
Carboxylated magnetic particle and the CA724 monoclonal antibody of chemiluminescent labels labelling.
In one embodiment, in the coated carboxylated magnetic particle of described CA724 monoclonal antibody, described sugar
Class antigen 724 monoclonal antibody is 1:25～35 with the mass ratio of described carboxylated magnetic particle.
In one embodiment, in the CA724 monoclonal antibody of described chemiluminescent labels labelling, described sugar
Class antigen 724 monoclonal antibody is 50:1～10 with the mass ratio of described chemiluminescent labels.
In one embodiment, the particle diameter of described carboxylated magnetic particle is 0.05 μm～1 μm.
In one embodiment, described chemiluminescent labels is luminol, different luminol, tris (bipyridine) ruthenium or acridine
Ester.
In one embodiment, also include that Chemoluminescent substrate, described Chemoluminescent substrate include A liquid and B liquid.
In one embodiment, described A liquid is H2O2Solution, described B liquid is NaOH solution.
In one embodiment, also include that CA724 calibrates product.
In one embodiment, described CA724 calibration product be concentration be respectively 0U/mL, 5U/mL, 20U/mL,
The solution of the CA724 of 50U/mL, 150U/mL and 300U/mL.
The preparation method of a kind of above-mentioned CA724 chemiluminescence immune detection reagent kit, comprises the steps:
Taking the suspension of carboxylated magnetic particle, Magneto separate is resuspended with MES buffer after removing supernatant, is subsequently added into EDC water
Solution, the surface carboxyl groups of the magnetic particle of activated carboxyl, it is subsequently added into CA724 monoclonal antibody, suspendible 2h under room temperature
～10h, Magneto separate is resuspended with Tris buffer after removing supernatant, obtains CA724 monoclonal antibody coated carboxylated
Magnetic particle；And
Take CA724 monoclonal antibody, mix after adding carbonate buffer solution, be subsequently adding chemiluminescent labels
Rear mixing, under room temperature, remove impurity after lucifuge reaction 1h～2h, obtains the CA724 monoclonal anti of chemiluminescent labels labelling
Body.
This CA724 chemiluminescence immune detection reagent kit can be with Full-automatic chemiluminescence immunoassay analysis meter
Detection instrument, completes the detection of CA724.This CA724 chemiluminescence immune detection reagent kit, Jing Guoshi
Testing, its detection sensitivity reaches 0.2U/mL, at least improves relative to the detection method sensitivity of traditional CA724
10 times, the accuracy of detection of this CA724 chemiluminescence immune detection reagent kit is higher.
Accompanying drawing explanation
Fig. 1 is the preparation stream of the preparation method of the CA724 chemiluminescence immune detection reagent kit of an embodiment
Journey schematic diagram；
Fig. 2 is the CA724 canonical plotting that embodiment 3 obtains.
Detailed description of the invention
Understandable for enabling the above-mentioned purpose of the present invention, feature and advantage to become apparent from, real with concrete below in conjunction with the accompanying drawings
Execute example the detailed description of the invention of the present invention is described in detail.Elaborate a lot of detail in the following description so that
Fully understand the present invention.But the present invention can implement to be much different from alternate manner described here, art technology
Personnel can do similar improvement in the case of intension of the present invention, and therefore the present invention is not embodied as by following public
Restriction.
The CA724 chemiluminescence immune detection reagent kit of one embodiment, including: CA724 monoclonal
The coated carboxylated magnetic particle of antibody and the CA724 monoclonal antibody of chemiluminescent labels labelling.
Preferably, in the coated carboxylated magnetic particle of CA724 monoclonal antibody, CA724 monoclonal
Antibody is 1:25～35 with the mass ratio of carboxylated magnetic particle.
Preferably, in the CA724 monoclonal antibody of chemiluminescent labels labelling, CA724 monoclonal
Antibody is 50:1～10 with the mass ratio of chemiluminescent labels.
Preferably, the particle diameter of carboxylated magnetic particle is 0.05 μm～1 μm.
Chemiluminescent labels can be luminol, different luminol, tris (bipyridine) ruthenium or acridinium ester.Wherein, chemiluminescence
Label is preferably acridinium ester.
In other examples, above-mentioned CA724 chemiluminescence immune detection reagent kit also includes chemiluminescence
Substrate solution.
Chemoluminescent substrate includes A liquid and B liquid.A liquid can be H2O2Solution, B liquid can be NaOH solution.
In the present embodiment, A liquid be concentration be the H of 0.1mol/L2O2Solution, B liquid be concentration be that the NaOH of 0.25mol/L is molten
Liquid.
In other examples, above-mentioned CA724 chemiluminescence immune detection reagent kit also includes sugar antigen
724 calibration product.
CA724 calibration product are that concentration is respectively 0U/mL, 5U/mL, 20U/mL, 50U/mL, 150U/mL and 300U/
The solution of the CA724 of mL.
Concrete, CA724 calibration product can use standard substance buffer that CA724 is configured to concentration to divide
Wei the solution of CA724 of 0U/mL, 5U/mL, 20U/mL, 50U/mL, 150U/mL and 300U/mL.
This CA724 chemiluminescence immune detection reagent kit, when CA724 detects, utilizes full-automatic
CA724 calibration product are detected by chemical illumination immunity analysis instrument, draw standard curve, are built in computer software；Connect
Test actual sample, calculate concentration of specimens according to sample luminous value；Finally to the immunity of CA724 Full-automatic chemiluminescence
Analysis system carries out the evaluation of performance (sensitivity, linear, precision, interference).
This CA724 chemiluminescence immune detection reagent kit can be with Full-automatic chemiluminescence immunoassay analysis meter
Detection instrument, completes the detection this CA724 chemiluminescence immune detection reagent kit of CA724, through experiment,
Its detection sensitivity reaches 0.2U/mL, at least improves 10 relative to the detection method sensitivity of traditional CA724
Times, the accuracy of detection of this CA724 chemiluminescence immune detection reagent kit is higher.
Additionally, this CA724 chemiluminescence immune detection reagent kit also has the advantage that
1, selection acridinium ester is as marker material, and is applied to chemiluminescence immunoassay system, and this luminescence system is straight
Connecing chemiluminescence, compared with traditional enzyme-catalyzed chemical luminescence, this reaction need not the participation of enzyme, more cost-effective；
2, select the chemiluminescence immunoassay system range of linearity width of acridinium ester, 0.2U/mL～300U/mL can be reached,
And the inspection range of linearity of the detection method of traditional CA724 is 2U/mL～100U/mL；
3, acridinium ester chemiluminescent immunoassay system repeatability is high, and in batch and difference between batch is all within 5%, this is other
Chemiluminescence immunoassay system is unapproachable；
4, chemiluminescence immunoassay system has realized the quantitative of sample, by built-in standard curve to test software, only
Need test sample just can directly obtain the concentration value of sample；
5, chemiluminescence immunoassay system can realize the interpolation of full-automation, reagent and sample has instrument to complete entirely,
Operate easier, decrease artificial error.
In conjunction with Fig. 1, the preparation method of above-mentioned CA724 chemiluminescence immune detection reagent kit, comprise the steps:
Taking the suspension of carboxylated magnetic particle, Magneto separate is resuspended with MES buffer after removing supernatant, is subsequently added into EDC water
Solution, the surface carboxyl groups of the magnetic particle of activated carboxyl, it is subsequently added into CA724 monoclonal antibody, suspendible 2h under room temperature
～10h, Magneto separate is resuspended with Tris buffer after removing supernatant, obtains CA724 monoclonal antibody coated carboxylated
Magnetic particle；And
Take CA724 monoclonal antibody, mix after adding carbonate buffer solution, be subsequently adding chemiluminescent labels
Rear mixing, under room temperature, remove impurity after lucifuge reaction 1h～2h, obtains the CA724 monoclonal anti of chemiluminescent labels labelling
Body.
The concentration of MES (2-(N-morpholine) ethyl sulfonic acid) buffer be 0.02M, pH be 5.5.
The concentration of Tris buffer is 0.1M and is 8.0 containing 2%BSA, pH.
The concentration of EDC (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides) aqueous solution is 10mg/mL～20mg/
ML, EDC are 0.05:0.1～1 with the mass ratio of carboxylated magnetic particle.
Preferably, in the coated carboxylated magnetic particle of CA724 monoclonal antibody, CA724 monoclonal
Antibody is 1:25～35 with the mass ratio of carboxylated magnetic particle.
Preferably, the particle diameter of carboxylated magnetic particle is 0.05 μm～1 μm.
The CA724 monoclonal antibody carbonate buffer solution concentration of chemiluminescent labels labelling is that 0.1M, pH are
9.0～9.5,
The operation of remove impurity is centrifugal desalting column desalination, and concrete operations are: the most respectively with pure water and TBS buffer (40mM
Tris-HCl, 0.5%BSA, 1%NaCl, pH 8.0) process centrifugal desalting column, it is eventually adding the CA724 Dan Ke obtained
The solution of the coated carboxylated magnetic particle of grand antibody, finally collects the liquid in centrifuge tube.
Preferably, in the CA724 monoclonal antibody of chemiluminescent labels labelling, CA724 monoclonal
Antibody is 50:1～10 with the mass ratio of chemiluminescent labels.
Chemiluminescent labels can be luminol, different luminol, tris (bipyridine) ruthenium or acridinium ester.Wherein, chemiluminescence
Label is preferably acridinium ester.
The coated carboxylated magnetic particle of CA724 monoclonal antibody that obtains and chemiluminescent labels labelling
CA724 monoclonal antibody cocktail i.e. can get above-mentioned CA724 chemiluminescence immune detection reagent kit.
This CA724 chemiluminescence immune detection reagent kit is in use, in addition it is also necessary to Chemoluminescent substrate and
CA724 calibration product.
Chemoluminescent substrate and CA724 calibration product can be prepared voluntarily and obtain.
Chemoluminescent substrate includes A liquid and B liquid.A liquid can be H2O2Solution, B liquid can be NaOH solution.
In the present embodiment, A liquid be concentration be the H of 0.1mol/L2O2Solution, B liquid be concentration be that the NaOH of 0.25mol/L is molten
Liquid.
Concrete, CA724 calibration product can use standard substance buffer that CA724 is configured to concentration to divide
Wei the solution of CA724 of 0U/mL, 5U/mL, 20U/mL, 50U/mL, 150U/mL and 300U/mL.
The preparation method of this CA724 chemiluminescence immune detection reagent kit is simple and convenient, and the saccharide prepared resists
The detection sensitivity of former 724 chemiluminescence immune detection reagent kits is higher, has a good application prospect.
It it is below specific embodiment.
Embodiment 1: the preparation of CA724 chemiluminescence immune detection reagent kit
(1) preparation of the coated carboxylated magnetic particle of CA724 monoclonal antibody:
Take containing the carboxylated magnetic particle (MagnaBind that 50mg particle diameter is 0.05 μm～1 μmTM, article No. 21353) suspend
Liquid, Magneto separate removes supernatant, is that 5.5MES buffer is resuspended with 0.02M, pH, and the EDC adding 10mg/mL newly configured for 1mL is water-soluble
Liquid, activated magnetic beads surface carboxyl groups, add 4mg CA724 monoclonal antibody (biorbyt, article No. orb48780), under room temperature
Suspendible 6h, Magneto separate, remove supernatant, be resuspended to 1mg/mL with the Tris buffer that the 0.1M containing 2%BSA, pH are 8.0, obtain
The coated carboxylated magnetic particle of CA724 monoclonal antibody, every bottle of 5mL subpackage be stored in 4 DEG C standby.
(2) preparation of the acridinium ester of inhibin labeling of monoclonal antibody:
Taking the CA724 monoclonal antibody that 50 μ L concentration are 25mg/mL, adding 150 μ L concentration is that 0.1M, pH are
The carbonate buffer solution of 9.0～9.5, mixing, it is subsequently adding the acridinium ester solution mixing that 1.5 μ L concentration are 5mg/mL, under room temperature
Lucifuge is reacted, and takes out, be centrifuged desalting column desalting processing with the zeba of 2mL, use pure water in desalination processes the most respectively after 1.5h
And TBS buffer processes, it is eventually adding the acridinium ester solution of the inhibin labeling of monoclonal antibody obtained, collects centrifuge tube
In liquid be in control the acridinium ester of inhibin labeling of monoclonal antibody to preserving, every bottle of 5mL subpackage be stored in 4 DEG C standby.
(3) preparation of CA724 calibration product:
With standard substance buffer (40mM Tris-HCl, 0.5%BSA, 1%NaCl, pH 8.0), CA724 is joined
Being set to concentration is 0U/mL, 5U/mL, 20U/mL, 50U/mL, 150U/mL and 300U/mL, every bottle of 0.5mL subpackage lyophilizing, 4 DEG C of guarantors
Deposit standby.
Embodiment 2: CA724 chemical luminous immune detection method
It is detection instrument with Full-automatic chemiluminescence immunoassay analysis meter (YHLO, article No. iFlash3000), methodology pattern
The coated carboxyl of CA724 monoclonal antibody of the sample of 50 μ L, 50 μ L it is sequentially added into for double antibody sandwich method, i.e. instrument
The magnetic particle changed and the CA724 treatment fluid of 50 μ L, after reaction 20min, then the CA724 adding 50 μ L is coated
Acridinium ester, after reaction 20min, carries out Magneto separate, and reactant mixture is sent into darkroom by instrument, is sequentially added into luminous substrate A liquid
(H2O2) and B liquid (NaOH) carry out luminescence-producing reaction, finally record luminous value.
Embodiment 3: CA724 chemiluminescence immune detection reagent kit performance evaluation
Use the method in embodiment 2 that CA724 calibration product are detected, obtain drawing standard curve such as Fig. 2
Shown in.
Then to then testing actual sample, concentration of specimens is calculated according to sample luminous value.
The detection of sensitivity:
With reference to CLSI EP17-A file recommendation experimental program, calculate CA724 chemiluminescence immune detection reagent kit
Sensitivity, the sensitivity tried to achieve is 0.2U/mL.
Linear detection:
It is that 0U/mL, 5U/mL, 20U/mL, 50U/mL, 150U/mL and 300U/mL standard substance do linear analysis to concentration, meter
Calculating linearly dependent coefficient, r=0.9996, it addition, this test kit is 0.2U/ to the range of linearity of CA724 sample detection
ML～300U/mL.
Precision measures:
Taking concentration is 50U/mL and two CA724 samples of 100U/mL, and each concentration of each sample is respectively done 3 and put down
OK, detecting with three batches of test kits, calculate test kit and criticize interior and difference between batch, result shows that this test kit is criticized interior and difference between batch is equal
Less than 5%.
Interference is tested:
Take pooled serum to add chaff interference respectively and include: conjugated bilirubin, unconjugated bilirubin, hemoglobin, ascorbic acid,
Glyceride, adds mass ratio and carries out according to 1:20, pooled serum after measuring pooled serum respectively and with the addition of various chaff interference
Measured value, calculates deviation therebetween, with ± 10% as tolerance interval.Result shows, interference all reaches the file of NCCLS
Standard, can be used for the accurate evaluation of clinical laboratory's CA724 situation.
Embodiment 4, the contrast experiment of CA724 chemiluminescence immune detection reagent kit
It is 0U/mL, 5U/mL, 20U/ by chemical luminescence detection method and traditional enzyme linked immunosorbent assay to concentration respectively
The CA724 sample of mL, 50U/mL, 150U/mL and 300U/mL detects, and two kinds of method detection sensitivities are compared, data
As shown in the table:
As can be seen from the above table, the sensitivity of chemical luminescence detection method relatively enzyme linked immunosorbent assay improves about 10 times.
Embodiment described above only have expressed the several embodiments of the present invention, and it describes more concrete and detailed, but also
Therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that, for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement, these broadly fall into the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a CA724 chemiluminescence immune detection reagent kit, it is characterised in that including: CA724 monoclonal
The coated carboxylated magnetic particle of antibody and the CA724 monoclonal antibody of chemiluminescent labels labelling.
CA724 chemiluminescence immune detection reagent kit the most according to claim 1, it is characterised in that described sugar
In the coated carboxylated magnetic particle of class antigen 724 monoclonal antibody, described CA724 monoclonal antibody and described carboxyl
The mass ratio of the magnetic particle changed is 1:25～35.
CA724 chemiluminescence immune detection reagent kit the most according to claim 1, it is characterised in that describedization
Learn in the CA724 monoclonal antibody of luminescent label, described CA724 monoclonal antibody and described chemistry
The mass ratio of luminous marker is 50:1～10.
CA724 chemiluminescence immune detection reagent kit the most according to claim 1, it is characterised in that described carboxylic
The particle diameter of the magnetic particle of base is 0.05 μm～1 μm.
CA724 chemiluminescence immune detection reagent kit the most according to claim 1, it is characterised in that describedization
Luminous marker is luminol, different luminol, tris (bipyridine) ruthenium or acridinium ester.
CA724 chemiluminescence immune detection reagent kit the most according to claim 1, it is characterised in that also include
Chemoluminescent substrate, described Chemoluminescent substrate includes A liquid and B liquid.
CA724 chemiluminescence immune detection reagent kit the most according to claim 6, it is characterised in that described A liquid
For H2O2Solution, described B liquid is NaOH solution.
CA724 chemiluminescence immune detection reagent kit the most according to claim 1, it is characterised in that also include
CA724 calibration product.
CA724 chemiluminescence immune detection reagent kit the most according to claim 8, it is characterised in that described sugar
Class antigen 724 calibrate product be concentration be respectively 0U/mL, 5U/mL, 20U/mL, 50U/mL, 150U/mL and 300U/mL saccharide resist
The solution of former 724.
10. one kind according to the CA724 chemiluminescence immune detection reagent kit according to any one of claim 1～9
Preparation method, it is characterised in that comprise the steps:
Taking the suspension of carboxylated magnetic particle, Magneto separate is resuspended with MES buffer after removing supernatant, is subsequently added into EDC aqueous solution,
The surface carboxyl groups of the magnetic particle of activated carboxyl, is subsequently added into CA724 monoclonal antibody, suspendible 2h～10h under room temperature,
Magneto separate is resuspended with Tris buffer after removing supernatant, obtains the coated carboxylated magnetic of CA724 monoclonal antibody micro-
Grain；And
Take CA724 monoclonal antibody, mix after adding carbonate buffer solution, mixed after being subsequently adding chemiluminescent labels
Even, under room temperature, remove impurity after lucifuge reaction 1h～2h, obtains the CA724 monoclonal antibody of chemiluminescent labels labelling.
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| CN110240644A (en) * | 2019-06-28 | 2019-09-17 | 深圳市亚辉龙生物科技股份有限公司 | Growth hormone receptor mutant, human growth hormone (HGH) immunogene, polyclonal antibody and detection kit |
| CN115494244A (en) * | 2022-11-21 | 2022-12-20 | 保定佳瑞源生物芯片有限公司 | Acridinium ester antibody marker diluent of cancer antigen CA724 and application thereof |
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