CN106124493A - The preparation method and applications of molecule imprinting hydrogel photon crystal micro-ball - Google Patents
The preparation method and applications of molecule imprinting hydrogel photon crystal micro-ball Download PDFInfo
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- CN106124493A CN106124493A CN201610437542.4A CN201610437542A CN106124493A CN 106124493 A CN106124493 A CN 106124493A CN 201610437542 A CN201610437542 A CN 201610437542A CN 106124493 A CN106124493 A CN 106124493A
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Abstract
The invention discloses a kind of boron affinity molecule trace hydrogel photonic crystal microsphere, it is characterized in that the inverse opal hydrogel body structure surface of described microsphere and the internal trace having molecules of interest, this structure can be combined with molecules of interest under condition of different pH or dissociate, and described molecule imprinting hydrogel photon crystal micro-ball is prepared via a method which: with photon crystal micro-ball as template, to fill in the hole between photon crystal micro-ball nanoparticle after oligomer solution mixing before hydrogel, when after hydrogel gelation, utilize the colloidal nanoparticles in caustic corrosion microsphere, remove microsphere template simultaneously, gained is molecule imprinting hydrogel photon crystal micro-ball.This microsphere can be during in the range of wider pH, (5.0 ~ 9.0) be applied to the multivariate detection technical field of the biomolecule containing cis-form dihydroxy structure such as glucose, protein.
Description
Technical field
The invention belongs to technical field of biological, be specifically related to the preparation side of a kind of molecular imprinting photonic crystal hydrogel
Method, this kind of microsphere can be applied to the multivariate detection of the biomolecule containing cis-form dihydroxy structure in wider pH range, as saccharide,
The detection of protein.
Background technology
Along with developing rapidly of medical skill, medical research has been enter into the epoch of molecular level.Traditional Molecular Detection side
Unimolecule can only be detected by method, and numerous disease is generally the most relevant with different kinds of molecules, and traditional method seems efficiency at this moment
Lowly, and with high costs, it is therefore desirable to a kind of high-throughout test platform.High-throughout experiment porch has then required high flux
Molecular vehicle identify different molecules, the most so-called cataloged procedure.
Liquid-phase chip technology with photon crystal micro-ball as carrier is in bioanalysis and protein, gene, drug screening
In obtained increasing utilization.Relative to the carrier of other forms, photon crystal micro-ball has significant advantage: first,
The specific surface area of microsphere is big, by increasing capacitance it is possible to increase effecting reaction surface to volume ratio, and the chemical reaction on surface therefore can be made to exist
Carry out in less volume；Second, use microsphere some other supplementary meanss can be utilized to wash away such as stirring, liquid as carrier
Etc. realizing a kind of reaction system between solid-liquid reaction and liquid-liquid reactions, thus accelerate the response speed of system；The
Three, microsphere relies on the intrinsic reflection peak of photonic crystal as its coded system, and coded system is simple, stable, and is easy to decoding.
4th, along with the change of microsphere surface functionalization group, the purposes of microsphere can be extended.Such as by cementing to photonic crystal and water-setting
It is together as carrier to carry out encoding the focus becoming the concern of the field such as high flux screening and combinatorial chemistry.
The principle of molecular imprinting is that target molecule and hydrogel are mixed post polymerization jointly, and eluting removes trace afterwards
Molecule, utilizes the trace stayed to realize the detection to microsphere, has the highest specificity and sensitivity.In the last few years,
The achievement in research that molecular engram is relevant increases substantially, it has also become a hot technology of materialogy.Phenylboric acid molecule is that one can
With with the molecule containing cis-form dihydroxy structure, as glucose, glycoprotein etc. combine in the basic conditions, and at acid condition
Under dissociate.Being added before hydrogel in oligomer solution as function monomer by phenylboric acid molecule, phenylboric acid can be with microsphere
Cis-form dihydroxy structure combines, and by regulating pH and removing template being cleaned multiple times after forming hydrogel, just can obtain phenylboric acid
Molecule imprinting hydrogel.Phenylboric acid molecule imprinting hydrogel can increase the active force between microsphere, thus promotes inspection
The specificity surveyed and sensitivity, and realize the repeatability that carrier uses.
Utilize photon crystal micro-ball for template, after preparing counter opal structure hydrogel, the advantage that microsphere is new can be given:
First, the specific surface area of hydrogel is big, and the chemical reaction on surface therefore can be made to be easier to make for；Second, water-setting can be controlled
Glue shrinks and diastole controls its volume, to extend its purposes；3rd, the activity of albumen can be kept with hydrogel for carrier,
Improve the stability of detection；4th, along with the change of hydrogel surface functionalization group, the purposes of hydrogel can be extended, carry
Rise specificity and sensitivity.
Summary of the invention
In order to solve to detect in existing label-free detection poor selectivity, high in cost of production shortcoming, the present invention provides one to prepare
For the method for the molecular imprinting photonic crystal hydrogel microsphere of multivariate detection, its preparation is simple, and with low cost, size uniformly may be used
Control, favorable repeatability.
Present invention provide the technical scheme that
A kind of molecule imprinting hydrogel photon crystal micro-ball, it is characterised in that the inverse opal hydrogel structure of described microsphere
Surface and the internal trace having molecules of interest, this structure can be combined with molecules of interest under condition of different pH or dissociate.Described
Molecule imprinting hydrogel photon crystal micro-ball is prepared via a method which: with photon crystal micro-ball as template, by poly-before hydrogel
Fill in the hole between photon crystal micro-ball nanoparticle after liquid solution mixing, when, after hydrogel gelation, utilizing corrosion
Colloidal nanoparticles in agent corrosion microsphere, removes microsphere template simultaneously, and it is brilliant that gained is molecule imprinting hydrogel photon
Body microsphere.
Preferably, described hydrogel is selected from one or more any mixing of boronic acid containing group hydrogel.
The invention provides the preparation method of a kind of molecule imprinting hydrogel photon crystal micro-ball for multivariate detection, its
It is characterised by said method comprising the steps of:
(1) photon crystal micro-ball template is prepared: monodispersed colloidal crystal nanoparticle adds deionized water dilution and forms glue
Liquid solution, utilizes micro fluidic device to make colloid solution be cut into monodispersed drop in continuous phase, solidifies drop template, clearly
Wash, after calcining, photon crystal micro-ball can be obtained；
(2) photon crystal micro-ball surface is carried out hydrophilic treated；
(3) photon crystal micro-ball is put in the hydrogel polymeric precursor solution containing microsphere and phenylboric acid, when micro-
The color of ball from white become bright-coloured colored time cured hydrogel, then hydrogel is soaked in deionized water, according to micro-
Degrees of expansion different inside and outside ball peels off photon crystal micro-ball from hydrogel；
(4) photon crystal micro-ball is thrown to deployed caustic, removes colloidal particle template and microsphere template,
And be stored in PBS.
Preferably, the micro fluidic device used in described method is coflow formula or convergence type micro fluidic device, micro-fluidic dress
The pipeline material put selects silicon dioxide, Teflon, one or more the combination in any of PDMS.
Preferably, the colloid solution used in described method is selected from silica colloidal particles solution, colloidal tio 2
One or more any mixing in particle solution and polystyrenic polymer's colloidal particle solution, colloidal particle
Size controlling is between 50nm-1000nm.
Preferably, described method is removed the caustic of drop template selected from Fluohydric acid., NaOH or pyrosulfuric acid in step (4)
Potassium, the one of tetrahydrofuran solution.
Molecule imprinting hydrogel photon crystal micro-ball is simple antistructure hydrogel.This molecule imprinting hydrogel photon is brilliant
Body microsphere has biological applications, such as biological multiplexed molecular detection etc..The molecule imprinting hydrogel photon crystal micro-ball of preparation,
Its size uniform, controllable.Molecule imprinting hydrogel photon crystal micro-ball is for the polynary inspection of the biomolecule such as glucose, protein
During survey, detecting step is as follows:
(1) photon crystal micro-ball template is prepared: monodispersed colloidal crystal nanoparticle adds deionized water dilution and forms glue
Liquid solution, utilizes micro fluidic device to make colloid solution be cut into monodispersed drop in continuous phase, solidifies drop template, clearly
Wash, after calcining, photon crystal micro-ball can be obtained；
(2) photon crystal micro-ball surface is carried out hydrophilic treated；
(3) photon crystal micro-ball is put in the hydrogel polymeric precursor solution containing microsphere and phenylboric acid, when micro-
The color of ball from white become bright-coloured colored time cured hydrogel, then hydrogel is soaked in deionized water, according to micro-
Degrees of expansion different inside and outside ball peels off photon crystal micro-ball from hydrogel；
(4) photon crystal micro-ball is thrown to deployed caustic, removes colloidal particle template and microsphere template,
And be stored in PBS.
(5) hydrogel microsphere prepared is taken out, after absorbing unnecessary liquid, join in detected sample solution mixed
Close, be allowed to, with the molecules of interest in sample solution, sufficient specific reaction occurs, after washing, detect reacted colloidal crystal
The reflectance spectrum of hydrogel microsphere, by comparing the color of colloidal crystal hydrogel microsphere before and after reaction or by measuring Colloidal crystals
The reflectance spectrum of body hydrogel microsphere, determines the content of test substance.
Preferably, the micro fluidic device used in described method is coflow formula or convergence type micro fluidic device, micro-fluidic dress
The pipeline material put selects silicon dioxide, Teflon, one or more the combination in any of PDMS.
Preferably, the colloid solution used in described method is selected from silica colloidal particles solution, colloidal tio 2
One or more any mixing in particle solution and polystyrenic polymer's colloidal particle solution, colloidal particle
Size controlling is between 50nm-1000nm.
Preferably, described method is removed the caustic of drop template selected from Fluohydric acid., NaOH or pyrosulfuric acid in step (4)
Potassium, the one of tetrahydrofuran solution.
Preferably, when carrying out detection application, the reflection peak of described microsphere hydrogel can be used for biomolecule coding.
Preferably, when carrying out detection application, the hydrogel trace of described microsphere is fixed biomolecule to be measured, and is caused water
The change of gel micro-ball reflection peak.
Relative to scheme of the prior art, the invention have the advantage that
(1) molecule imprinting hydrogel photon crystal micro-ball of the present invention utilize the reflection peak position of photonic crystal as coding,
Along with colloid particle size is different, the position of reflection peak can comprise whole visible region, it might even be possible to is infrared or ultraviolet
Region.
(2) present invention utilizes molecule imprinting hydrogel technology, the specific surface area of hydrogel is bigger, can be to trace sample
Product detect, and meet the requirement being suitable for high sensitivity carrier, can obtain high detection sensitivity；The present invention utilizes molecular engram
Method is combined with molecules detected, cross reaction seldom occurs, therefore has the highest specificity；The present invention utilizes phenylboric acid to make
For function monomer, it is 5.0～about 9.0 detections to the molecule containing cis-form dihydroxy structure at pH, and in acid condition may be used
Inverse property is dissociated, thus realizes the repeatability of detection；Due to the catoptric arrangement of photonic crystal, carrier surface is to fluorescently-labeled glimmering
The absorption of light reduces, it is possible to improve intensity and the sensitivity of detection reaction of fluorescence.
(3) molecule imprinting hydrogel photon crystal micro-ball of the present invention can in the range of wider pH (5.0～9.0) to containing
The multivariate detection of the biomolecule of cis-form dihydroxy structure such as glucose, protein.Under normal circumstances, blood of human body, saliva, eye
Tear, the pH scope of urine are 7.4,6.2-7.4,6.5-7.6 and 4.5-8.0.The present invention almost covers blood, saliva, tear, urine
PH value range, it is not necessary to the pH of these samples is adjusted, easy to operate and reduce the wind of component degradation of instability
Danger.
Accompanying drawing explanation
Below in conjunction with the accompanying drawings and embodiment the invention will be further described:
Fig. 1 be phenylboric acid molecule imprinting hydrogel photon crystal micro-ball prepare schematic diagram.
Fig. 2 be internal (c), low concentration of water gel (d) after the positive body structure surface (a) of photon crystal micro-ball, internal (b), encapsulating,
High-concentration hydrogel (e) and high-concentration hydrogel cross section (f).
Detailed description of the invention
Embodiment 1 is with SiO2Photonic crystal is that the phenylboric acid molecule imprinting hydrogel microsphere of template is prepared and in Radix Cochleariae officinalis mistake
Application in the detection of oxide enzyme:
1. the preparation of orderly photon crystal micro-ball: by monodispersed SiO2Nanoparticle adds deionized water regulation concentration extremely
20%-30%；Homemade glass micro-fluidic device is utilized to make colloid solution cut into monodispersed drop, by liquid in flowing mutually
Drip template and be placed in 75 DEG C of dry solidifications of baking oven, after removing the impurity of surface or inside, be placed in 800 DEG C of calcining 4h in Muffle furnace.
2. the hydrophilic treated of colloidal crystal microsphere: first the colloidal crystal microsphere after calcining is soaked in volume ratio is 7:3
Concentrated sulphuric acid and hydrogen peroxide mixed liquor in 12h so that microsphere surface hydroxylating, repeatedly clean colloidal crystal microsphere with ultra-pure water,
And dry up under the stream of nitrogen gas of 0.02MPa.
3. the preparation of phenylboric acid hydrogel polymeric precursor solution: weigh 10mg horseradish peroxidase, and by Radix Cochleariae officinalis peroxide
The PBS of compound enzyme 0.1M pH7.0 is configured to the enzymatic solution of 100 μ L；Take 2 μ L light triggers, 1mg phenylboric acid, 40 μ L
The PBS mixing of polyethylene glycol 200 and 40 μ L 0.1M pH7.0, is subsequently adding the enzymatic solution of 20 μ L, 50 μ L Polyethylene Glycol
The PBS of diacrylate 700 and 48 μ L 0.1M pH7.0, blending incubation 10 minutes under room temperature, gained is phenylboric acid
Hydrogel polymeric precursor solution.
4. phenylboric acid hydrogel is filled: colloidal crystal microsphere soaked in phenylboric acid hydrogel polymeric precursor solution, to micro-
Ball becomes colored from white, irradiates polymerization 5～10s with strong ultraviolet light.Then phenylboric acid hydrogel is dipped to deionization water-soluble
In liquid, due to the coefficients of expansion different inside and outside microsphere, gap can be formed at microsphere surface, it is easy to just can be by microsphere from water-setting
Glue is stripped out.
5. template is removed: the SiO being filled with phenylboric acid after being completely exfoliated2Microsphere is dipped in HF solution, HF solution
Can homogeneous corrosion SiO from outside to inside2Particle, ultimately forms phenylboric acid hydrogel microsphere；Owing to being sour environment, phenylboric acid molecule
Dissociate with microsphere.
6. the removal of imprint molecule: repeatedly clean No. three microspheres with the PBS of 0.1M pH7.0 and remove trace
Molecule, final acquisition molecule imprinting hydrogel photon crystal micro-ball.
7. the drafting of horseradish peroxidase standard curve: the molecule imprinting hydrogel photon crystal micro-ball of gained is measured
After reflection peak, being dipped in the horseradish peroxidase solution of 100 μ L variable concentrations, fully reaction is measured after 30 minutes again
Reflection peak also calculates reflection peak change, draws horseradish peroxidase concentration standard curve according to the data obtained.
8. the detection of horseradish peroxidase in pair liquid to be measured: the molecule imprinting hydrogel photon crystal micro-ball of gained is surveyed
After determining reflection peak, being dipped in 100 μ L solution to be measured, fully reaction was again measured reflection peak and is calculated reflection peak after 30 minutes
Change, carries out contrasting according to the data obtained and horseradish peroxidase standard curve thus obtains horseradish peroxidase in liquid to be measured
The concentration of enzyme.
Embodiment 2 is with SiO2Photonic crystal is that the phenylboric acid molecule imprinting hydrogel microsphere of template is prepared and at ribose core
Application in acid enzyme B detection:
1. the preparation of orderly photon crystal micro-ball: by monodispersed SiO2Nanoparticle adds deionized water regulation concentration extremely
20%-30%；Homemade glass micro-fluidic device is utilized to make colloid solution cut into monodispersed drop, by liquid in flowing mutually
Drip template and be placed in 75 DEG C of dry solidifications of baking oven, after removing the impurity of surface or inside, be placed in 800 DEG C of calcining 4h in Muffle furnace.
2. the hydrophilic treated of colloidal crystal microsphere: first the colloidal crystal microsphere after calcining is soaked in volume ratio is 7:3
Concentrated sulphuric acid and hydrogen peroxide mixed liquor in 12h so that microsphere surface hydroxylating, repeatedly clean colloidal crystal microsphere with ultra-pure water,
And dry up under the stream of nitrogen gas of 0.02MPa.
3. the preparation of phenylboric acid hydrogel polymeric precursor solution: weigh 10mg ribonuclease B, and by ribonuclease B
The enzymatic solution of 100 μ L it is configured to the PBS of 0.1M pH7.0；Take 2 μ L light triggers, 1mg phenylboric acid, the 40 poly-second of μ L two
The PBS mixing of alcohol 200 and 40 μ L 0.1M pH7.0, is subsequently adding the enzymatic solution of 20 μ L, 50 μ L Polyethylene Glycol two propylene
The PBS of acid esters 700 and 48 μ L 0.1M pH7.0, blending incubation 10 minutes under room temperature, gained is phenylboric acid hydrogel
Polymeric precursors solution.
4. phenylboric acid hydrogel is filled: colloidal crystal microsphere soaked in phenylboric acid hydrogel polymeric precursor solution, to micro-
Ball becomes colored from white, irradiates polymerization 5～10s with strong ultraviolet light.Then phenylboric acid hydrogel is dipped to deionization water-soluble
In liquid, due to the coefficients of expansion different inside and outside microsphere, gap can be formed at microsphere surface, it is easy to just can be by microsphere from water-setting
Glue is stripped out.
5. template is removed: the SiO being filled with phenylboric acid after being completely exfoliated2Microsphere is dipped in HF solution, HF solution
Can homogeneous corrosion SiO from outside to inside2Particle, ultimately forms phenylboric acid hydrogel microsphere；Owing to being sour environment, phenylboric acid molecule
Dissociate with microsphere.
6. the removal of imprint molecule: repeatedly clean No. three microspheres with the PBS of 0.1M pH7.0 and remove trace
Molecule, final acquisition molecule imprinting hydrogel photon crystal micro-ball.
7. the drafting of ribonuclease B standard curve: the molecule imprinting hydrogel photon crystal micro-ball of gained is measured anti-
After penetrating peak, being dipped in the ribonuclease B solution of 100 μ L variable concentrations, fully reaction measures reflection after 30 minutes again
Peak also calculates reflection peak change, draws the concentration standard curve of ribonuclease B according to the data obtained.
8. the detection of ribonuclease B in pair liquid to be measured: the molecule imprinting hydrogel photon crystal micro-ball of gained is measured
After reflection peak, being dipped in 100 μ L solution to be measured, fully reaction is again measured reflection peak and calculates reflection peak change after 30 minutes
Change, carry out contrasting according to the data obtained and ribonuclease B standard curve thus obtain the dense of ribonuclease B in liquid to be measured
Degree.
Embodiment 3 is with SiO2Photonic crystal is the phenylboric acid molecule imprinting hydrogel microsphere preparation of template and is turning ferrum egg
Application in white enzyme detection:
1. the preparation of orderly photon crystal micro-ball: by monodispersed SiO2Nanoparticle adds deionized water regulation concentration extremely
20%-30%；Homemade glass micro-fluidic device is utilized to make colloid solution cut into monodispersed drop, by liquid in flowing mutually
Drip template and be placed in 75 DEG C of dry solidifications of baking oven, after removing the impurity of surface or inside, be placed in 800 DEG C of calcining 4h in Muffle furnace.
2. the hydrophilic treated of colloidal crystal microsphere: first the colloidal crystal microsphere after calcining is soaked in volume ratio is 7:3
Concentrated sulphuric acid and hydrogen peroxide mixed liquor in 12h so that microsphere surface hydroxylating, repeatedly clean colloidal crystal microsphere with ultra-pure water,
And dry up under the stream of nitrogen gas of 0.02MPa.
3. the preparation of phenylboric acid hydrogel polymeric precursor solution: weigh 10mg transferrins enzyme, and transferrins enzyme is used
The PBS of 0.1M pH7.0 is configured to the enzymatic solution of 100 μ L；Take 2 μ L light triggers, 1mg phenylboric acid, 40 μ L Polyethylene Glycol
The PBS mixing of 200 and 40 μ L 0.1M pH7.0, is subsequently adding the enzymatic solution of 20 μ L, 50 μ L Polyethylene Glycol diacrylate
The PBS of ester 700 and 48 μ L 0.1M pH7.0, blending incubation 10 minutes under room temperature, gained is phenylboric acid hydrogel and gathers
Close precursor solution.
4. phenylboric acid hydrogel is filled: colloidal crystal microsphere soaked in phenylboric acid hydrogel polymeric precursor solution, to micro-
Ball becomes colored from white, irradiates polymerization 5～10s with strong ultraviolet light.Then phenylboric acid hydrogel is dipped to deionization water-soluble
In liquid, due to the coefficients of expansion different inside and outside microsphere, gap can be formed at microsphere surface, it is easy to just can be by microsphere from water-setting
Glue is stripped out.
5. template is removed: the SiO being filled with phenylboric acid after being completely exfoliated2Microsphere is dipped in HF solution, HF solution
Can homogeneous corrosion SiO from outside to inside2Particle, ultimately forms phenylboric acid hydrogel microsphere；Owing to being sour environment, phenylboric acid molecule
Dissociate with microsphere.
6. the removal of imprint molecule: repeatedly clean No. three microspheres with the PBS of 0.1M pH7.0 and remove trace
Molecule, final acquisition molecule imprinting hydrogel photon crystal micro-ball.
7. the drafting of transferrins enzyme standard curve: the molecule imprinting hydrogel photon crystal micro-ball of gained is measured reflection
Behind peak, being dipped in the transferrins enzymatic solution of 100 μ L variable concentrations, fully reaction measures reflection peak also after 30 minutes again
Calculating reflection peak changes, and draws transferrins enzyme concentration standard curve according to the data obtained.
8. the detection of transferrins enzyme in pair liquid to be measured: the molecule imprinting hydrogel photon crystal micro-ball of gained is measured anti-
After penetrating peak, being dipped in 100 μ L solution to be measured, fully reaction is again measured reflection peak and calculates reflection peak change after 30 minutes
Change, carry out contrasting according to the data obtained and transferrins enzyme standard curve thus obtain the concentration of transferrins enzyme in liquid to be measured.
Examples detailed above, only for technology design and the feature of the explanation present invention, its object is to allow the person skilled in the art be
Will appreciate that present disclosure and implement according to this, can not limit the scope of the invention with this.All according to present invention essence
God's equivalent transformation of being done of essence or modification, all should contain within protection scope of the present invention.
Claims (8)
1. a molecule imprinting hydrogel photon crystal micro-ball, it is characterised in that the inverse opal hydrogel structural table of described microsphere
Face and the internal trace having molecules of interest, this structure is combined with molecules of interest under condition of different pH or dissociates.
The preparation method of a kind of molecule imprinting hydrogel photon crystal micro-ball the most according to claim 1, it is characterised in that
Described method realizes according to the following steps:
(1) photon crystal micro-ball template is prepared: it is molten that monodispersed colloidal crystal nanoparticle adds deionized water dilution formation colloid
Liquid, utilizes micro fluidic device to make colloid solution be cut into monodispersed drop in continuous phase, solidifies drop template, clean,
After calcining, photon crystal micro-ball can be obtained；
(2) photon crystal micro-ball surface is carried out hydrophilic treated；
(3) photon crystal micro-ball is put in the hydrogel polymeric precursor solution containing microsphere and phenylboric acid, when microsphere
Color from white become bright-coloured colored time cured hydrogel, then hydrogel is soaked in deionized water, according in microsphere
Outer different degrees of expansion peels off photon crystal micro-ball from hydrogel；
(4) photon crystal micro-ball is thrown to deployed caustic, remove colloidal particle template and microsphere template, and protect
It is stored in PBS.
Method the most according to claim 2, it is characterised in that the micro fluidic device used in step (1) is coflow formula or remittance
The poly-flow control apparatus that declines, the pipeline material of micro fluidic device select silicon dioxide, Teflon, PDMS one or more
Combination in any.
Method the most according to claim 2, it is characterised in that the colloid solution used in step (1) described method is dioxy
SiClx colloidal particle, titania colloid particles or polystyrenic polymer's solution；The particle size range of colloidal particle is at 50nm-
Between 1000nm.
Method the most according to claim 2, it is characterised in that the caustic removing drop template in step (3) is hydrogen fluorine
Acid, NaOH or potassium pyrosulfate, oxolane.
6. one kind by the application of the molecule imprinting hydrogel photon crystal micro-ball described in claim 1, it is characterised in that described
Application is the multivariate detection in the range of pH5.0 ~ 9.0 to the biomolecule containing cis-form dihydroxy structure.
Application the most according to claim 6, it is characterised in that the described biomolecule containing cis-form dihydroxy structure is Portugal
Grape sugar, protein etc..
Application the most according to claim 6, it is characterised in that when carrying out detection application, described molecule imprinting hydrogel structure
Combine with corresponding biomolecule when trace, can tie with corresponding microsphere when detection after removing this biomolecule
Close, and realize detection by the reflection peak change in location surveying hydrogel.
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| CN112225856A (en) * | 2020-10-26 | 2021-01-15 | 南京鼓楼医院 | Inverse opal structure microsphere with multiple molecular imprinting and preparation method and application thereof |
| CN112264115A (en) * | 2020-10-26 | 2021-01-26 | 南京鼓楼医院 | Fishbone microfluidic chip carrying molecular imprinting inverse opal structure microspheres and preparation method thereof |
| CN115124888A (en) * | 2021-03-24 | 2022-09-30 | 中国科学院化学研究所 | Ink-jet printing photonic crystal microarray, biological detection chip, preparation method and application thereof |
| CN115746200A (en) * | 2022-10-21 | 2023-03-07 | 四川大学 | Gel microsphere capable of adsorbing and decomposing bilirubin, preparation method and application thereof |
-
2016
- 2016-06-17 CN CN201610437542.4A patent/CN106124493A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| HUAN WANG 等: "《Boronate Affinity Molecularly Imprinted Inverse Opal Particles for Multiple Label-Free Bioassays》", 《CHEMICAL COMMUNICATIONS》 * |
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