CN106124444A - A kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process - Google Patents
A kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process Download PDFInfo
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- CN106124444A CN106124444A CN201610422315.4A CN201610422315A CN106124444A CN 106124444 A CN106124444 A CN 106124444A CN 201610422315 A CN201610422315 A CN 201610422315A CN 106124444 A CN106124444 A CN 106124444A
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- 238000000034 method Methods 0.000 title claims abstract description 45
- 230000008569 process Effects 0.000 title claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 230000005059 dormancy Effects 0.000 claims abstract description 22
- 235000016623 Fragaria vesca Nutrition 0.000 claims abstract description 7
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims abstract description 7
- 239000002689 soil Substances 0.000 claims abstract description 7
- 239000010453 quartz Substances 0.000 claims abstract description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000004458 analytical method Methods 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims abstract description 4
- 241000372132 Hydrometridae Species 0.000 claims abstract description 3
- 238000001228 spectrum Methods 0.000 claims description 12
- 241000220223 Fragaria Species 0.000 claims description 6
- 238000012935 Averaging Methods 0.000 claims description 3
- 230000003595 spectral effect Effects 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 235000021028 berry Nutrition 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 239000011521 glass Substances 0.000 abstract description 2
- 230000035515 penetration Effects 0.000 abstract description 2
- 239000004033 plastic Substances 0.000 abstract description 2
- 229920003023 plastic Polymers 0.000 abstract description 2
- 238000002203 pretreatment Methods 0.000 abstract description 2
- 240000009088 Fragaria x ananassa Species 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000012937 correction Methods 0.000 description 3
- 238000010606 normalization Methods 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002790 cross-validation Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000019371 dormancy process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013569 fruit product Nutrition 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process, the method comprises the steps: that the data recorded using Abbe refractometer, as reference value, are set up, in conjunction with the PLS method in TQAnalyst7.1, the NIR that in Fructus Fragariae Ananssae root system, irreducible water and Free water measure and analyzed model；In Fructus Fragariae Ananssae dormancy season, periodically excavate rhizosphere of strawberry soil, expose side root system and the system of fibrous root；Prepare sample, load quartz specimen cup, use integrating sphere diffuse-reflectance test sample, utilize NIR to analyze model analysis and obtain the degree of depth of Fructus Fragariae Ananssae dormancy.The present invention detects process simple and fast, near infrared light has the strongest penetration capacity and scattering effect, is made without any pre-treatment when detection, can penetrate glass, quartz and plastics package and directly detect, the most not using any chemical reagent, result is more objective, accurate.
Description
Technical field
The present invention relates to root system of plant observation technology field, be specifically related to a kind of employing near infrared device and measure open country Fructus Fragariae Ananssae
The method of sleeping process.
Background technology
Open country Fructus Fragariae Ananssae needs after natural dormancy, could normal rudiment, bloom, even if otherwise giving suitable environment
Condition, also not rudiment is bloomed, even if rudiment sometimes but irregular, and growth result is bad.
The sleeping process of Fructus Fragariae Ananssae is directly connected to the lifting of the accumulation of self chilling requirement, yield and fruit quality.It is so far
Only, the judgement for Fructus Fragariae Ananssae sleeping process is only limitted to the method differentiation of the size variation by observing bud (leaf bud)；But, adopt
Can only judge that dormancy terminates early stage roughly in this way, it is impossible to sleeping process (dormancy induction period, the deep-sleep phase, stop
The dormancy releasing phase) dynamically to observe, this is to clear and definite Fructus Fragariae Ananssae sleeping process and regulation and control chilling requirement, to increase yield, to promote fruit product
Matter.Having scholar to propose when bud increases, irreducible water starts to change to Free water；Also scholar is had to think, with Free water in sprout cell
The increase of increase and bud there is concordance, and bud size determines that the short-cut method whether Fructus Fragariae Ananssae dormancy terminates.Plant physiology
Showing, the dormancy of Fructus Fragariae Ananssae real meaning is the dormancy of root system, and the dormancy of sprout is the apparent phenomenon of dormancy, to this end, exactly
Verifying the sleeping process of Fructus Fragariae Ananssae, the dormancy situation tackling its root system is studied.Document shows, in Fructus Fragariae Ananssae root system dormancy and root system
The degree of freedom of water is closely related, and i.e. when the irreducible water of root system is far longer than Free water, Fructus Fragariae Ananssae initially enters dormancy；When root system from
By water movable hardly time, Fructus Fragariae Ananssae Fructus Fragariae Ananssae is in deep-sleep period；When in root system, Free water is far longer than irreducible water, Fructus Fragariae Ananssae
Start breaking dormancy.
Summary of the invention
It is an object of the invention to overcome the above-mentioned problems in the prior art, it is provided that a kind of employing near infrared device is surveyed
The method determining open country Fructus Fragariae Ananssae sleeping process, is understood by irreducible water and the content of Free water and degree of freedom in mensuration Fructus Fragariae Ananssae root system
Fructus Fragariae Ananssae root system active situation, for judging that the sleeping process of Fructus Fragariae Ananssae provides reliable basis.
For realizing above-mentioned technical purpose, reaching above-mentioned technique effect, the present invention is to be achieved through the following technical solutions:
A kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process, the method comprises the steps:
(1) foundation of model: taking the Fructus Fragariae Ananssae root system of the different upgrowth situation of many groups, each group of Fructus Fragariae Ananssae root system is divided into two parts, its
Middle portion utilizes Abbe refractometer to record root system irreducible water and Free water data, and another part utilizes NIR (near-infrared) to diffuse
The NIR spectra of spectral technology strawberrying root system sample, the data recorded using Abbe refractometer as reference value, in conjunction with
PLS (partial least square method) method in TQAnalyst7.1 is set up the NIR that in Fructus Fragariae Ananssae root system, irreducible water and Free water measure and is analyzed
Model, and verify with unknown sample；
(2) in Fructus Fragariae Ananssae dormancy season, periodically excavate rhizosphere of strawberry soil, expose side root system and the system of fibrous root；
(3) in selecting step (2), the side root system of appropriate length and the system of fibrous root, as sample, load quartz specimen cup, the reddest
External spectrum instrument uses integrating sphere diffuse-reflectance test sample, and resolution is 8cm-1, scan 64 times, sweep limits 12000～4000cm-1, temperature
Spend 23-27 DEG C, relative humidity 15%-20%；Obtained NIR spectra is carried out pretreatment and feature analysis, utilizes step (1)
In NIR analyze model, analyze and obtain the degree of depth of Fructus Fragariae Ananssae dormancy.
In the present invention, described Fructus Fragariae Ananssae dormancy is limited to March in November in winter to next year in season.
In the present invention, described step excavated rhizosphere of strawberry soil every 2 weeks in (2).
In the present invention, described step (2) excavates degree of depth 10-20cm of rhizosphere of strawberry soil.
In the present invention, in described step (3), every batch sample repeats fill sample and scan 5 times, is averaging spectrum.
Near infrared device operation principle: near infrared light refers to wavelength electromagnetism between visible region and middle infrared
Ripple, its wave-length coverage is about 780～2500nm, and wave-number range is about 12500～4000cm-1.Use near infrared device, come from red
The infrared light that outside line emitter sends produces pulse signal, and the transition that this pulse signal comes from molecular vibration energy level is the most adjoint
Rotational energy level transition and produce.What it recorded is frequency multiplication and the sum of fundamental frequencies information of the frequency vibration of single chemical bond in molecule, mainly
Owing to the difference on irreducible water and the position of Free water absworption peak and intensity, reaching to reflect that in root system, water belongs to irreducible water still
Free water, the situation that the simultaneous reactions system of fibrous root expands.
The invention has the beneficial effects as follows:
1, detection process simple and fast, near infrared light has the strongest penetration capacity and scattering effect, is not required to when detection
Carry out any pre-treatment, glass, quartz and plastics package can be penetrated and directly detect, the most do not use any chemistry examination
Agent, result is more objective, accurate.
2, can complete monitoring sleeping process, can only judge that roughly Fructus Fragariae Ananssae stops by Fructus Fragariae Ananssae sprout size when overcoming perusal
The deficiency of dormancy process.
Certainly, the arbitrary product implementing the present invention it is not absolutely required to reach all the above advantage simultaneously.
Accompanying drawing explanation
In order to be illustrated more clearly that the technical scheme of the embodiment of the present invention, embodiment will be described required use below
Accompanying drawing is briefly described, it should be apparent that, the accompanying drawing in describing below is only some embodiments of the present invention, for ability
From the point of view of the those of ordinary skill of territory, on the premise of not paying creative work, it is also possible to obtain the attached of other according to these accompanying drawings
Figure.
Fig. 1 is that in the present embodiment, 10 batches of Fructus Fragariae Ananssae root systems scan the NIR spectra stacking chart after 5 times.
Detailed description of the invention
The following examples are only used for explaining the present invention, and the unrestricted present invention.Based on the embodiment in the present invention, ability
All other embodiments that territory those of ordinary skill is obtained under not making creative work premise, broadly fall into the present invention and protect
The scope protected.
Embodiment one
Gather NIR spectra: the side root system and the system of fibrous root that take appropriate length are divided into 10 batches of Fructus Fragariae Ananssae root system samples, are respectively charged into stone
English specimen cup, near infrared spectrometer uses integrating sphere diffuse-reflectance test sample, and resolution is 8cm-1, scan 64 times, sweep limits 12000
～4000cm-1, temperature 23-27 DEG C, relative humidity 15%-20%；Every batch sample repeats fill sample and scan 5 times, is averaging spectrum,
See Fig. 1.
The pretreatment of NIR spectra: be respectively adopted first derivative (first derivative), second dervative (second
Derivative), multiplicative scatter correction (mutiplicative signal correction, MSC), vector normalization,
The methods such as big minimum normalization are to Pretreated spectra, with the cross-validation coefficient of determination (R2), correction mean square deviation (RMSEC)
It is aggregative indicator evaluation with prediction mean square deviation (RMSEP).R2Closer to 1, illustrate that sample analysis value is with NIR predictive value dependency more
Good.RMSEC is the least, and surface model returns the best.RMSEP is the least, shows that model prediction ability is the strongest.Requirement in the present embodiment
1 ＜ RMSEP/RMSEC ＜ 1.2, result see table 1:
The impact of table 1 preprocess method
Selecting of number of principal components: make full use of spectral information when rationally determining the number of principal components participating in modeling and filter noise
One of effective ways.With RMSECV as parameters optimization, when RMSECV value is minimum, selected number of principal components is optimal, model pre-
Survey precision the highest.Result shows, RMSECV minima is 0.2412, and corresponding main cause subnumber is 10, is shown in Table 2:
The impact of table 2 preprocess method
| Number of principal components | 5 | 6 | 7 | 8 |
| RMSECV | 0.3328 | 0.3261 | 0.2899 | 0.2674 |
| Number of principal components | 9 | 10 | 11 | 12 |
| RMSECV | 0.2493 | 0.2412 | 0.2637 | 0.2619 |
Set up model: use the PLS method in TQAnalyst7.1 to set up model, use SNV+ first derivative to carry out pre-place
Reason, selects 4486.27～7408.30cm-1Wave band, number of principal components 10, set up model.R2=0.9748, RMSEC=0.134,
RMSEP=0.147, RMSEP/RMSEC=1.097, illustrate model stability, accurately, and this NIR analyzes model and can be used for Fructus Fragariae Ananssae root system
Irreducible water and the quantitative analysis of Free water, and then analyze the degree of depth obtaining Fructus Fragariae Ananssae dormancy.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.Preferred embodiment is the most detailed
Describe all of details, be also not intended to the detailed description of the invention that this invention is only described.Obviously, according to the content of this specification,
Can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is to preferably explain the present invention
Principle and actual application so that skilled artisan can be best understood by and utilize the present invention.The present invention is only
Limited by claims and four corner thereof and equivalent.
Claims (5)
1. one kind uses the method that near infrared device measures open country Fructus Fragariae Ananssae sleeping process, it is characterised in that the method includes as follows
Step:
(1) foundation of model: taking the Fructus Fragariae Ananssae root system of the different upgrowth situation of many groups, each group of Fructus Fragariae Ananssae root system is divided into two parts, Qi Zhongyi
Part utilizing Abbe refractometer to record root system irreducible water and Free water data, another part utilizes the NIR spectral technology that diffuses to gather grass
The NIR spectra of certain kind of berries root system sample, the data recorded using Abbe refractometer are as reference value, in conjunction with the PLS in TQAnalyst7.1
Method is set up the NIR that in Fructus Fragariae Ananssae root system, irreducible water and Free water measure and is analyzed model, and verifies with unknown sample；
(2) in Fructus Fragariae Ananssae dormancy season, periodically excavate rhizosphere of strawberry soil, expose side root system and the system of fibrous root；
(3) in selecting step (2), the side root system of appropriate length and the system of fibrous root, as sample, load quartz specimen cup, near infrared light
Spectrometer uses integrating sphere diffuse-reflectance test sample, and resolution is 8cm-1, scan 64 times, sweep limits 12000～4000cm-1, temperature
23-27 DEG C, relative humidity 15%-20%；Obtained NIR spectra is carried out pretreatment and feature analysis, utilizes in step (1)
NIR analyze model, analyze and obtain the degree of depth of Fructus Fragariae Ananssae dormancy.
A kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process the most according to claim 1, its feature
Being, described Fructus Fragariae Ananssae dormancy is limited to March in November in winter to next year in season.
A kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process the most according to claim 1, its feature
Being, described step excavated rhizosphere of strawberry soil every 2 weeks in (2).
A kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process the most according to claim 1, its feature
It is, described step (2) excavates degree of depth 10-20cm of rhizosphere of strawberry soil.
A kind of method using near infrared device to measure open country Fructus Fragariae Ananssae sleeping process the most according to claim 1, its feature
Being, in described step (3), every batch sample repeats fill sample and scan 5 times, is averaging spectrum.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106645037A (en) * | 2016-11-17 | 2017-05-10 | 安徽理工大学 | Method for detecting heavy metal content of coal gangue filling reclamation reconstruction soil based on high spectrum technology |
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| WO2006068597A1 (en) * | 2004-12-22 | 2006-06-29 | Astrazeneca Ab | Spectroscopic method |
| US20130025350A1 (en) * | 2010-01-28 | 2013-01-31 | Oji Paper Co., Ltd. | Method and device for measuring basis weight and moisture content amount |
| CN101806730A (en) * | 2010-04-13 | 2010-08-18 | 江苏大学 | Vinegar residue organic matrix moisture content detection method |
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| CN106645037A (en) * | 2016-11-17 | 2017-05-10 | 安徽理工大学 | Method for detecting heavy metal content of coal gangue filling reclamation reconstruction soil based on high spectrum technology |
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Effective date of registration: 20230208 Address after: 230000 Tianfeng Strawberry Garden, Feigang Village, Shuihu Town, Changfeng County, Hefei City, Anhui Province Patentee after: Hefei Tianfeng strawberry Co.,Ltd. Address before: Room 516, Innovation Building, Anhui Academy of Agricultural Sciences, No. 40, Nongke South Road, Hefei, Anhui Province, 230031 Patentee before: INSTITUTE OF GARDENING, ANHUI ACADEMY OF AGRICULTURAL SCIENCES |