CN106119338A - Colpitic test kit of a kind of detection and preparation method thereof - Google Patents
Colpitic test kit of a kind of detection and preparation method thereof Download PDFInfo
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- CN106119338A CN106119338A CN201610485081.8A CN201610485081A CN106119338A CN 106119338 A CN106119338 A CN 106119338A CN 201610485081 A CN201610485081 A CN 201610485081A CN 106119338 A CN106119338 A CN 106119338A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
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Abstract
It is desirable to provide a kind of be capable of quickly detecting, accuracy in detection is high and is capable of vaginitis test kit and the preparation method of this test kit of entry detection, the method is simple to operate, and the test kit quality prepared is good.Described test kit includes detecting clamp; described detection clamp is provided with several detection holes, has (adjacent) Nitrobenzol N acetyl β D glucosaminide (NGA), 5 bromines 4 chlorine 3 indole acetyl neuraminic acid (SNA), 5 bromines 4 chlorine 3 ethychlozate ester (LE), 5 bromines 4 chlorine 3 indole glucuronide salt (GUS), peroxidase (H at these several detection hole endoperidium2O2) or pH indicator at least one；The preparation method of this test kit includes soaking, is dried, is installed, pushes up flat, packaging and other steps, finally obtains the test kit that packaging of aluminium foil bag is good.Present invention can apply to biochemistry detection technical field.
Description
Technical field
The present invention relates to a kind of biochemistry detection technical field, particularly relate to the colpitic test kit of a kind of detection, and should
The preparation method of test kit.
Background technology
Vagina, from the body cavity that angle of physiology is a Guan Bi, comprises Multiple components in it, also parasitic
Many antibacterials.Microecology in vaginas mainly has three parts to constitute, and is first vaginal epithelial cell, and Part II is microorganism species,
3rd it must be emphasized that Tiny ecosystem part be between host and flora and the complexity of the interphase interaction of flora and flora.They
Between be mutually restriction, interact, the complementary unity of opposites, or symbiosis or the antagonism relationship, be co-located in the moon
In the microecological environment in road, remain the state of coordinated balance.
Normal vagina endoparasitism lactobacillus, streptococcus, staphylococcus epidermidis, gardnerella vaginalis and escherichia coli with
And the Peptostreptococcus of anaerobism, dyspepsiacoccus, anaerobism lactobacillus, bacteroid etc., these bacterium keep certain ratio, maintain
Intravaginal ecological balance, in normal women vaginal secretions, every milliliter has 106 colony-forming units.
Normal women of child-bearing age, under the effect of endocrine hormone, vaginal epithelial cell hypertrophy, its cells of superficial layer contains abundant
Glycogen, be very beneficial for hold concurrently oxygen lactobacillus growth, this antibacterial accounts for more than the 90% of vagina, and this lactobacillus is a large amount of
Exist, may refrain from the growth of other pathogenic bacterium, defined a normal ecological balance in intravaginal.Lactobacillus is belonging to
The antibacterial of lactic acid bacteria one class, this kind of bacterium may utilize flavoprotein and oxygen become H2O2, this effect combines heme, peroxide
Change the disappearance of hydrogen enzyme, the H that result produces excess and all do not degraded by antibacterial2O2.Its concentration is relevant with the quantity of lactobacillus.
Thus can be by detection H2O2Concentration reflect the situation of lactobacillus.H in normal female vaginal secretions2O2Concentration one
As more than 2 μm ol/L, as detection H2O2During μm ol/L of concentration≤2, then mean that vagina ecology flora has suffered to destroy,
Cause the growth of anaerobe, result in the unbalance of vaginal microbial flora.As: when human body decrease in estrogen, cause vagina epithelium
Atrophy, cell glycogen reduces, is unfavorable for the growth of lactobacillus；Use antibiotic in a large number or excessively rinse the moon with akaline liquid
Road, the growth of suppression lactobacillus；Property disorderly, frequency of sexual intercourse (because seminal fluid pH is 7.2-7.8) etc. cause pathogenic anaerobic bacteria and
Gardnerella amount reproduction, causes the imbalance of vagina microorganism ecological balance, produces vaginitis.
Vaginitis is the modal disease of women, and cervicitis there is also higher sickness rate.When inflammatory patient oneself's symptom
Time smaller, easily cause mistaken diagnosis, usually delay treatment, and likely concurrent salpingitis, endometritis and cause
Infertile and miscarriage.
In order to detect colpitic type rapidly, it would be highly desirable to a kind of novel detection kit so that detection is quickly
Accurately, convenient means are provided for follow-up treatment.
Summary of the invention
The technical problem to be solved is to overcome the deficiencies in the prior art, it is provided that one is capable of quickly examining
Survey, accuracy in detection is high and is capable of the vaginitis test kit of entry detection.
Present invention also offers the preparation method of a kind of mentioned reagent box, the method is simple to operate, the reagent prepared
Box quality is good, and detection efficiency is high.
The colpitic test kit of a kind of detection of the present invention be the technical scheme is that this test kit includes detection card
Plate, is provided with several detection holes on described detection clamp, has (adjacent) Nitrobenzol-N-at these several detection hole endoperidium
Acetyl-β-bromo-the 4-of D-glucosamine glycosides NAG, 5-chloro-3-bromo-4-of indole acetyl neuraminic acid SNA, 5-chloro-3-indole second
The bromo-4-of acid esters LE, 5-chloro-3-indole glucuronide salt GUS, peroxidase H2O2Or at least one in pH indicator.
Such scheme is visible, and the detection clamp on this test kit arranges some detection holes, and this is capable of multiple project
Detection, can only realize with existing single detection card individual event visual inspection survey compared with, the structure of this detection clamp is designed to greatly
Ground improves detection efficiency, it is achieved disposable entry detection, and is capable of quickly detecting, and entry carries out the inspection of identical index
Survey, it is also possible to improve accuracy in detection, provide basis for estimation accurately for follow-up treatment.
Further, the content being coated thing in described detection hole is as follows:
To (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides NAG 2%；
The bromo-4-of 5-chloro-3-indole acetyl neuraminic acid SNA 3%；
The bromo-4-of 5-chloro-3-indolyl acetic acid ester LE 2%；
The bromo-4-of 5-chloro-3-indole glucuronide salt GUS 2%；
Peroxidase H2O2300U/L。
Such scheme is visible, in detection hole to be coated species more, and content disclosure satisfy that detection demand, it is possible to realizes
Quickly detection and the accuracy of guarantee testing result.
Yet further, the number in described detection hole is five, is coated with respectively (adjacent) in these five described detection holes
Nitrobenzol-N-acetyl-β-bromo-the 4-of D-glucosamine glycosides NAG, 5-chloro-3-indole bromo-4-of acetyl neuraminic acid SNA, 5-
Chloro-3-indolyl acetic acid ester LE, peroxidase H2O2With pH indicator；As can be seen here, five detection holes are used, it is possible to realize five
Joint inspection is surveyed, and meets the detection of five kinds of different indexs, thus realizes entry detection.
The most further, the number in described detection hole is six, and it is right to be coated with respectively in these six described detection holes
(adjacent) Nitrobenzol-N-acetyl-β-bromo-4-of D-glucosamine glycosides NAG, 5-chloro-3-indole acetyl neuraminic acid SNA, 5-is bromo-
The 4-bromo-4-of chloro-3-indolyl acetic acid ester LE, 5-chloro-3-indole glucuronide salt GUS, peroxidase H2O2Indicate with pH
Agent；As can be seen here, use six detection holes, it is possible to realize six joint inspections and survey, meet the detection of six kinds of different indexs, thus realize many
Project detects.Additionally, the number in described detection hole can be arranged as required to, this does not limit.
The another kind of scheme that the present invention provides is the preparation method of above-mentioned detection kit, and the method comprises the following steps:
(1) detection paper soaks: being placed in by detection paper and be coated in thing immersion uniformly, taking-up is dried, and the thing that is coated therein is for (adjacent)
Nitrobenzol-N-acetyl-β-bromo-the 4-of D-glucosamine glycosides NAG, 5-chloro-3-indole bromo-4-of acetyl neuraminic acid SNA, 5-
The bromo-4-of chloro-3-indolyl acetic acid ester LE, 5-chloro-3-indole glucuronide salt GUS, peroxidase H2O2With pH indicator；
(2) dried detection paper is loaded in the corresponding detection hole of described detection card；
(3) utilize pressuring flat device to be put down in described detection hole on detection paper top, make detection paper be adjacent to the bottom in described detection hole；
(4) the detection card after the flat detection in top paper is carried out packaging of aluminium foil bag, finally obtain the colpitic test kit of described detection.
Such scheme is visible, and the preparation of test kit of the present invention has only to through soaking, be dried, be installed, push up flat, bag
Dress, can finally obtain the test kit that packaging of aluminium foil bag is good, and its preparation technology is simple, It greatly reduces the labor of staff
Fatigue resistance, reduces cost；The test kit quality that it prepares is preferable, it is possible to realize detecting fast and accurately.
More specifically, the pressuring flat device in described step (3) is for manually or automatically changing machinery；As can be seen here, adopt
Automatically pushing up flat with the flat machinery realization of automatically pushing up with thimble, its efficiency is improved further, and employing equipment enters
Row top is flat, and it pushes up flat effect and can be guaranteed, and pushes up flat works very well.
Accompanying drawing explanation
Fig. 1 is the easy structure schematic diagram of the detection clamp with five detection holes of the present invention；
Fig. 2 is the easy structure schematic diagram of the detection clamp with six detection holes of the present invention.
Detailed description of the invention
As Fig. 1 is to shown in 2, and in the present embodiment, test kit of the present invention includes detecting clamp 1, at described detection card
Being provided with five detection holes or six detection holes on plate, this is according to concrete it needs to be determined that the number in detection hole, and this does not has
Limit, as 2 holes, 3 holes, 4 holes or more porous can be arranged.In the present embodiment, it is provided that the number in detection hole is five holes and six holes
Two kinds of situation explanations.Certainly, this is not limitation of the present invention.When for five detection holes, being coated in five detection holes
Thing is to (adjacent) Nitrobenzol-N-acetyl-β-bromo-4-of D-glucosamine glycosides NAG, 5-chloro-3-indole acetyl neuraminic acid
The bromo-4-of SNA, 5-chloro-3-indolyl acetic acid ester LE, peroxidase H2O2With pH indicator.When for six detection holes, these six
The thing that is coated in detection hole is to (adjacent) Nitrobenzol-N-acetyl-β-bromo-4-of D-glucosamine glycosides NAG, 5-chloro-3-indole second
The bromo-4-of acylneuraminate SNA, 5-bromo-4-of chloro-3-indolyl acetic acid ester LE, 5-chloro-3-indole glucuronide salt GUS, mistake
Oxide enzyme H2O2With pH indicator.The above-mentioned selection being coated thing can also have varied, is not unalterable, according to
Concrete needs and set and concrete be coated thing.
The above-mentioned content being coated thing is as follows:
To (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides NAG 2%；The bromo-4-of 5-chloro-3-indole acetyl group nerve ammonia
Acid SNA 3%；The bromo-4-of 5-chloro-3-indolyl acetic acid ester LE 2%；The bromo-4-of 5-chloro-3-indole glucuronide salt GUS 2%；
Peroxidase H2O2 300U/L。
The preparation method step of this test kit is as follows:
(1) detection paper soaks: being placed in by detection paper and be coated in thing immersion uniformly, taking-up is dried, and the thing that is coated therein is right
(adjacent) Nitrobenzol-N-acetyl-β-bromo-4-of D-glucosamine glycosides NAG, 5-chloro-3-indole acetyl neuraminic acid SNA, 5-is bromo-
The 4-bromo-4-of chloro-3-indolyl acetic acid ester LE, 5-chloro-3-indole glucuronide salt GUS, peroxidase H2O2Indicate with pH
Agent；
(2) dried detection paper is loaded in the corresponding detection hole of described detection card；
(3) utilize pressuring flat device to be put down in described detection hole on detection paper top, make detection paper be adjacent to the bottom in described detection hole；
Pressuring flat device therein is for manually or automatically changing machinery.
(4) the detection card after the flat detection in top paper is carried out packaging of aluminium foil bag, finally obtain the colpitic reagent of described detection
Box.
In the present invention, several choosing principles being coated thing are as follows.
Can be anti-with β-2-Acetamido-2-deoxy-D-glucose glycosides enzyme to (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides NAG
Should, reaction generate to (adjacent) Nitrobenzol displaing yellow in the basic conditions, the depth of color and β-2-Acetamido-2-deoxy-D-glucose glycosides
The content of enzyme is directly proportional.And the microorganism such as Candida albicans in vagina, trichomonal vaginitis, mycoplasma hominis can be secreted
β-2-Acetamido-2-deoxy-D-glucose glycosides enzyme.When by vagina extract and to (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine
Glycosides NAG reacts, and shows the situation that i.e. would know that extracted specimen according to reacted color.
Neuraminidase can hydrolyze the bromo-4-of 5-chloro-3-indole acetyl neuraminic acid SNA, discharges colourless bromo indole
Base, reacts when running into reactant in blue or green, and shade is directly proportional to the content of neuraminidase.And saliva
Liquid neuraminidase is the enzyme-specific of the BV pathogenic bacterium such as Gardnerella, Mobiluncus secretion.When vagina extract is bromo-with 5-
4-chloro-3-indole acetyl neuraminic acid SNA reacts, and shows the feelings that i.e. would know that extracted specimen according to reacted color
Condition.
Leukocyte esterase can hydrolyze the bromo-4-of 5-chloro-3-indolyl acetic acid ester LE, discharges colourless bromo indole base, runs into anti-
React when answering thing in blue or green, and shade is proportional with leukocyte esterase activity；Leukocyte esterase is many
The carboxy-lesterase of forming core leukocyte release, when being inflamed, polymorphonuclear leukocyte is assembled in focus and is discharged leukocyte in a large number
Esterase, all visible substantially polymorphonuclear leukocyte of bacillary and mycotic vaginosis, without this phenomenon time normal.Therefore when by vagina
Extract 4-bromo-with 5-chloro-3-indolyl acetic acid ester LE reacts, and shows the vaginitis that i.e. would know that women according to reacted color
Disease situation.
Beta-glucuronidase enzyme can hydrolyze the bromo-4-of 5-chloro-3-indole glucuronide salt GUS, discharges colourless
Bromo indole base, reacts when running into reactant in blue or green, and shade and beta-glucuronidase enzymatic activity
Proportional.And beta-glucuronidase enzyme is the enzyme-specific of the aerobe such as escherichia coli.Therefore when by vagina extract
4-bromo-with 5-chloro-3-indole glucuronide salt GUS reacts, and shows the moon that i.e. would know that women according to reacted color
Road inflammatory conditions.
Hydrogen peroxide is the mark of Vaginal lactobacilli.On the premise of catalase exists, hydrogen peroxide is demarcated
Producing oxygen, oxygen can make chromogenic substrate occur to be changed to mauve color by colourless, the concentration of shade and hydrogen peroxide in
Direct ratio.Therefore when by vagina extract and peroxidase H2O2Reaction, shows according to reacted color and i.e. would know that women
Colpitis situation.
And indicator substrate is when different pH-values on pH value pad, the different colours change that display is corresponding, thus realize
PH value indicates.
The present invention uses brand-new design, and detection clamp is designed with the form in multiple detection hole, and it can be carried out
The detection of many index, can be effectively improved detection efficiency；Its Detection accuracy is the most high.Compared with prior art, which solve
Existing single detection hole carries out detecting and causes the low problem of detection efficiency, also achieves multiple index simultaneously and detects simultaneously
Possibility.
Present invention can apply to biochemistry detection technical field.
Claims (6)
1. detect a colpitic test kit, including detection clamp (1), it is characterised in that: set on described detection clamp (1)
It is equipped with several detection holes, has (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides at these several detection hole endoperidium
(NAG), the bromo-4-of 5-chloro-3-indole acetyl neuraminic acid (SNA), the bromo-4-of 5-chloro-3-indolyl acetic acid ester (LE), the bromo-4-of 5-
Chloro-3-indole glucuronide salt (GUS), peroxidase (H2O2) or pH indicator at least one.
The colpitic test kit of a kind of detection the most according to claim 1, it is characterised in that: the bag in described detection hole
As follows by the content of thing:
To (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides (NAG) 2%；
The bromo-4-of 5-chloro-3-indole acetyl neuraminic acid (SNA) 3%；
The bromo-4-of 5-chloro-3-indolyl acetic acid ester (LE) 2%；
The bromo-4-of 5-chloro-3-indole glucuronide salt (GUS) 2%；
Peroxidase (H2O2) 300U/L.
The colpitic test kit of a kind of detection the most according to claim 2, it is characterised in that: the number in described detection hole is
Five, it is coated with respectively (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides (NAG), 5-in these five described detection holes
Bromo-4-chloro-3-indole acetyl neuraminic acid (SNA), the bromo-4-of 5-chloro-3-indolyl acetic acid ester (LE), peroxidase (H2O2)
With pH indicator.
The colpitic test kit of a kind of detection the most according to claim 2, it is characterised in that: the number in described detection hole is
Six, it is coated with respectively (adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides (NAG), 5-in these six described detection holes
Bromo-4-chloro-3-indole acetyl neuraminic acid (SNA), the bromo-4-of 5-chloro-3-indolyl acetic acid ester (LE), the bromo-4-of 5-chloro-3-indole
Glucuronide salt (GUS), peroxidase (H2O2) and pH indicator.
5. the preparation method of the colpitic test kit of detection as claimed in claim 1, it is characterised in that the method includes
Following steps:
(1) detection paper soaks: being placed in by detection paper and be coated in thing immersion uniformly, taking-up is dried, and the thing that is coated therein is right
(adjacent) Nitrobenzol-N-acetyl-β-D-glucosamine glycosides (NAG), the bromo-4-of 5-chloro-3-indole acetyl neuraminic acid (SNA),
The bromo-4-of 5-chloro-3-indolyl acetic acid ester (LE), the bromo-4-of 5-chloro-3-indole glucuronide salt (GUS), peroxidase
(H2O2) and pH indicator；
(2) dried detection paper is loaded in the corresponding detection hole of described detection card；
(3) utilize pressuring flat device to be put down in described detection hole on detection paper top, make detection paper be adjacent to the bottom in described detection hole；
(4) the detection card after the flat detection in top paper is carried out packaging of aluminium foil bag, finally obtain the colpitic test kit of described detection.
The preparation method of the colpitic test kit of detection the most according to claim 5, it is characterised in that: described step (3)
In pressuring flat device for manually or automatically changing machinery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610485081.8A CN106119338A (en) | 2016-06-28 | 2016-06-28 | Colpitic test kit of a kind of detection and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610485081.8A CN106119338A (en) | 2016-06-28 | 2016-06-28 | Colpitic test kit of a kind of detection and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110243817A (en) * | 2019-07-16 | 2019-09-17 | 迪瑞医疗科技股份有限公司 | A kind of GRD beta-glucuronidase drying chemical reagent paper and detection method |
-
2016
- 2016-06-28 CN CN201610485081.8A patent/CN106119338A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110243817A (en) * | 2019-07-16 | 2019-09-17 | 迪瑞医疗科技股份有限公司 | A kind of GRD beta-glucuronidase drying chemical reagent paper and detection method |
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