CN106119077B - Aseptic inoculation box - Google Patents

Aseptic inoculation box Download PDF

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Publication number
CN106119077B
CN106119077B CN201610511103.3A CN201610511103A CN106119077B CN 106119077 B CN106119077 B CN 106119077B CN 201610511103 A CN201610511103 A CN 201610511103A CN 106119077 B CN106119077 B CN 106119077B
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CN
China
Prior art keywords
aseptic inoculation
fuel
test tube
platform
nipple
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201610511103.3A
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Chinese (zh)
Other versions
CN106119077A (en
Inventor
王文博
苑学霞
李瑞菊
李慧冬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Agricultural Quality Standards and Testing Technology of Shandong Academy of Agricultural Sciences
Original Assignee
Institute of Agricultural Quality Standards and Testing Technology of Shandong Academy of Agricultural Sciences
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Publication date
Application filed by Institute of Agricultural Quality Standards and Testing Technology of Shandong Academy of Agricultural Sciences filed Critical Institute of Agricultural Quality Standards and Testing Technology of Shandong Academy of Agricultural Sciences
Priority to CN201610511103.3A priority Critical patent/CN106119077B/en
Publication of CN106119077A publication Critical patent/CN106119077A/en
Application granted granted Critical
Publication of CN106119077B publication Critical patent/CN106119077B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02Filters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

A kind of aseptic inoculation box provided by the invention, including upper shell, lower housing and aseptic inoculation platform, the upper shell upper side is transparent material, the upper shell is equipped with curtain, the lower housing leading flank left end is equipped with fuel cylinder, aseptic inoculation platform centre position is equipped with ventilation hole, fuel nipple is equipped on the upside of the ventilation hole, lower housing leading flank centre position is equipped with ignitor switch, the lower housing leading flank right end is equipped with battery pack, the ventilation hole lower end is equipped with air blower, quantity switch of air is equipped on the right side of the shell leading flank ignitor switch, filter membrane is equipped in the ventilation hole between air blower and fuel nipple, position forward at left and right sides of fuel nipple is equipped with cover board on the aseptic inoculation platform, aseptic inoculation platform lower part is equipped with test tube case.Aseptic inoculation box provided by the invention is convenient for carrying, simple operation, can ensure gnotobasis.

Description

Aseptic inoculation box
Technical field
The present invention relates to the experimental instrument and equipment technical field of experiment outdoor application, specifically a kind of aseptic inoculations Case.
Background technology
In agricultural production, the harmful microorganism to agricultural product production scene is needed to be detected, in production engineering Microbiological risk assessment and diversity of soil microorganism research etc..
Common method is to carry out spot sampling, then takes back laboratory and carries out culture assays, for detecting in agricultural product The projects such as total plate count, coliform, salmonella, Shigella, staphylococcus aureus, mould, yeast and commercial sterilization, In the lab, be currently to assist to create gnotobasis using superclean bench and alcolhol burner, either sampling process still Seeded process, the fungus block of picking or mycelia expose aerial overlong time in operating process, and pollution probability is big.Spot sampling Then laboratory is taken back to be inoculated with, it is not convenient enough, cross-infection is susceptible to during transport, there has been no direct at present The experimental facilities of aseptic inoculation is carried out at the scene.To solve the above-mentioned problems, the present invention provides one kind and is convenient for carrying, can be in reality It tests outdoor application and is capable of providing good gnotobasis, aseptic inoculation box convenient to use.
Invention content
It is an object of the invention to solve the above problems, design one kind be convenient for carrying, ship, it is easy to use, safety and The more thorough aseptic inoculation box of gnotobasis.
The technical scheme adopted by the invention to solve the technical problem is that:
A kind of aseptic inoculation box, including upper shell, lower housing and the aseptic inoculation positioned at upper shell and lower housing joining place Platform, the upper shell upper side are transparent material, and the upper shell two sides are equipped with window, curtain, institute are equipped at the window It states curtain to connect with shell at window, the curtain is soft curtain, and the soft curtain is made of transparent plastic material, the upper shell Unthreaded hole is equipped on the upside of window, soft curtain upper end position corresponding with unthreaded hole on the upside of window is equipped with the unthreaded hole of same size, described Soft curtain upper end is fixedly connected by bolt with upper shell, and the lower housing leading flank left end is equipped with fuel cylinder, and the fuel cylinder is solid It is scheduled on lower housing, aseptic inoculation platform centre position is equipped with ventilation hole, and fuel nipple, the combustion are equipped on the upside of the ventilation hole It is fixedly connected between material mouth and ventilation hole upside aseptic inoculation platform surface, the fuel nipple medial slope is sprayed equipped with flame Mouthful, the fuel cylinder is connected by fuel hose with the fuel nipple, and lower housing leading flank centre position is equipped with sparking and opens It closes, the ignitor switch is connected by conducting wire with fuel nipple, and the lower housing leading flank right end is equipped with battery pack, the battery pack Downside is equipped with babinet, and the battery pack is fixed in babinet, and the ventilation hole lower end is equipped with air blower, the blower drum with Ventilation hole inner wall is fixedly connected, and quantity switch of air is equipped on the right side of the shell leading flank ignitor switch, the quantity switch of air is by leading Line is connected with the air blower and battery pack, is equipped with filter membrane in the ventilation hole between air blower and fuel nipple, described sterile Position forward at left and right sides of fuel nipple is equipped with cover board in vaccination station, and the cover board quantity is each one of left and right, the cover board shape Shape is rectangle, and the both ends far from fuel nipple are respectively provided with two hinges, the cover board and aseptic inoculation platform on described two cover boards Between by hinge connection, the position on described two cover boards close to fuel nipple is respectively provided with equipped with a handle I, the nothing Bacterium vaccination station position corresponding with the cover board is equipped with rectangular counter sink, the counter sink upper blind bore inner wall and the lid The cooperation contact of plate side, aseptic inoculation platform lower part are equipped with test tube case, and the test tube case is the case of upper opening Body structure, the test tube case left and right sides are respectively provided with a handle II, and being equipped with test tube in the test tube case is clamped Frame, test tube holding frame both ends are weldingly fixed in test tube case, are fixed with test tube on the test tube holding frame, it is described on The both sides of shell are fixedly connected with shoulder strap, and rearward position is equipped with homogenizer on the left of the aseptic inoculation platform fuel nipple, described equal Matter device includes that hammering plate, handle and front end cap, the homogenizer are connected by conducting wire with battery pack, the homogenizer two sides The position of bottom end is equipped with cuboid protrusion I, and raised I centre position of the cuboid is equipped with unthreaded hole, on the aseptic inoculation platform with The corresponding position of raised I unthreaded hole of the cuboid is equipped with threaded hole, solid by screw I between the homogenizer and aseptic inoculation platform Fixed connection, aseptic inoculation platform fuel nipple right side rearward position are equipped with sample storage box, sample storage box two sides The position of bottom end is equipped with cuboid protrusion II, and raised II centre position of the cuboid is equipped with unthreaded hole, on the aseptic inoculation platform Position corresponding with raised II unthreaded hole of the cuboid is equipped with threaded hole, passes through between the sample storage box and aseptic inoculation platform Screw II is fixedly connected.
The homogenizer hammering plate is waveform, and homogenizer front end cap is full open formula glass transparent window door, Temperature sensor, temperature controller, heater and refrigerator are equipped in the sample storage box, the temperature sensor is by leading Line is connected with temperature controller, heater and refrigerator.
It is connected by buckle structure between the upper shell and lower housing, the quantity of the buckle is front and back each two, institute It is transparent glass to state upper shell upper side transparent material shell.
The fuel cylinder upper end is equipped with valve, is equipped with cylindrical box on the downside of the fuel cylinder, in the cylindrical box body Wall is contacted with the cooperation of fuel cylinder lower end outer surface, and the fuel cylinder centre position is equipped with semi-annular wall pipe, the semi-circular For wall pipe sleeve on fuel cylinder, the semi-annular wall pipe is fixed on lower housing leading flank by screw II.
It is fixed using welding manner between aseptic inoculation platform surface on the upside of the fuel nipple and ventilation hole, the fuel nipple is Annular, is uniformly equipped with flame jet port on the fuel nipple medial slope, the direction of the flame jet port axis with it is sterile Vaccination station surface angulation is 30 °, and lower housing leading flank centre position is equipped with ignitor switch, and the ignitor switch is electricity Sub- valve, the ignitor switch are connected by conducting wire with fuel nipple.
The beneficial effects of the invention are as follows:
1, aseptic inoculation box upper shell provided by the invention both sides are equipped with soft curtain, and aseptic inoculation platform centre position is equipped with ventilation Hole, the ventilation hole lower end are equipped with air blower, are equipped with fuel nipple on the upside of the ventilation hole, are set between the air blower and fuel nipple There are filter membrane, air to enter inside aseptic inoculation box by venthole under the action of air blower, filter membrane carries out just air The filtering of step carries out heating sterilization at fuel nipple to air, ensures the gnotobasis inside aseptic inoculation box, air is by soft Curtain is discharged inside aseptic inoculation box, generates pollution, improves inoculation survival rate and yield rate.
2, rearward position is equipped with homogenizer, aseptic inoculation platform right side rearward position on the left of the aseptic inoculation platform Equipped with sample storage box, aseptic inoculation platform lower part is equipped with test tube case, homogenizer, sample storage box, test tube case It is integrated in aseptic inoculation box, it is easy to use, only need carrying aseptic inoculation box that can complete to be inoculated in use, structure It is compact, it is small, it is light-weight, it transports and asks after the sampling and sampling that solve area that is long-range, remote or not having condition of culture Topic.
3, homogenizer hammering plate is designed as waveform so that the hammering plate in hammering sample homogenizing bag repeatedly, It can preferably cause to shake, and generate vortex in the solution, accelerate mixing, efficiently separate micro- life on solid sample surface Object, and microorganism in solution is made to be in uniform distribution.It can adjustment working time appropriate and row during hammering Crowded speed is come to renting the soft homogenate of cell realization.Homogenizer front cover board is set as full open formula glass transparent window door, It is readily cleaned, glass transparent window facilitates the situation in observation sample sack.Temperature sensor, temperature are equipped in the sample storage box Degree controller, heater and refrigerator, the temperature sensor pass through conducting wire and temperature controller, heater and refrigerator phase Even, the temperature sensor is used for incuding the temperature in sample storage box, the temperature controller control heater and refrigerator The temperature in sample storage box is controlled, it to the microbiological specimens being further processed to laboratory need to be sent, can be effective Ensure the true microbial status of submitted sample.
4, the upper shell upper side is transparent material, facilitates in seeded process and is observed, the upper shell and lower casing It is connected by buckle structure between body, when being operated without inoculation, upper shell can be taken down, facilitating will be in sample storage box Sample sack takes out, while just and just carrying out cleaning and sterilizing to aseptic inoculation box inside, is also convenient for zero in aseptic inoculation box Part repairs.
5, the shape of the fuel nipple is annular, radially even on the fuel nipple medial slope to be equipped with eight flames Jet port, direction and the aseptic inoculation platform surface angulation of the flame jet port axis are 30 °, can be more fully to drum Wind turbine is blown into the air inside aseptic inoculation platform and carries out sterilization antivirus, can preferably ensure gnotobasis.
6, the forward position in aseptic inoculation platform left and right ends is equipped with cover board, leads between the cover board and aseptic inoculation platform It crosses hinge connection, is equipped with handle I close to the position of fuel nipple on the cover board, a handle I is respectively provided on described two cover boards, Need to only be opened cover board during being inoculated with can take out test tube from test tube case, easy to operate, need not be from The external world is included in test tube, ensures the environment of a relative closure, prevents to infect.
7, described test tube case or so is respectively provided with one, and two outer surfaces of the test tube case are respectively provided with a handle Hand, the test tube in test tube case can extract test tube case out after all inoculation, be convenient for changing test tube, the examination Test tube holding frame is equipped in pipe case, test tube holding frame both ends are weldingly fixed in test tube case, and the test tube is logical It crosses test tube holding frame to fix, prevents toppling and rupturing for test tube, storage safety.
8, the both sides of the upper shell are fixedly connected with shoulder strap, and the shoulder strap can assist people to carry aseptic inoculation box, convenient It carries.
Description of the drawings
Fig. 1 is the outline structural diagram of the present invention;
Fig. 2 internal structure schematic diagrams of the present invention;
Fig. 3 is sectional view of the present invention;
Fig. 4 is homogenizer structural schematic diagram in the present invention;
Fig. 5 is soft curtain structure schematic diagram in the present invention;
Fig. 6 is fuel nipple structural schematic diagram in the present invention;
Fig. 7 is pilot scale tube grip frame structural schematic diagram of the present invention.
Specific implementation mode
As shown in Figures 1 to 7, a kind of aseptic inoculation box, including upper shell 1, lower housing 2 and be located at upper shell 1 and lower casing The aseptic inoculation platform 3 of 2 joining place of body, the aseptic inoculation platform 3 is horizontal positioned, and 1 upper side of the upper shell is transparent material, institute It is transparent glass to state transparent material, and convenient to be observed in being inoculated with operating process, the two sides of the upper shell 1 pass through D types Button is fixedly connected with shoulder strap 20, and the shoulder strap 20 is convenient for carrying, and 1 two sides of upper shell position on the lower side is equipped with window, Two windows are symmetrical arranged, and curtain 7 is equipped at the window, and the curtain 7 is soft curtain, and the soft curtain uses transparent plastic material system At equipped with unthreaded hole on the upside of 1 window of the upper shell, soft curtain upper end position corresponding with unthreaded hole on the upside of window is equipped with identical big Small unthreaded hole, the soft curtain upper end are fixedly connected by bolt 71 with upper shell 1, and the air in the aseptic inoculation platform 3 can be with It is discharged by soft curtain, hand can be deep into across soft curtain for inside aseptic inoculation box.Between the upper shell 1 and lower housing 2 It is connected by buckle structure 21, the buckle structure 21 is easy to disassemble, can disassemble upper shell 1, facilitate and store sample Sample sack in case takes out, while conveniently carrying out cleaning and sterilizing to aseptic inoculation box inside, is also convenient for in aseptic inoculation box Parts repair.
2 leading flank left end of the lower housing is equipped with fuel cylinder 5, and 5 upper end of the fuel cylinder is equipped with valve 54, the valve 54 Control fuel cylinder 5 opening and closing, 5 lower end of the fuel cylinder be equipped with cylindrical box 51,51 inner wall of the cylindrical box with it is described The cooperation contact of 5 lower end outer surface of fuel cylinder, 5 centre position of the fuel cylinder is equipped with semi-annular wall pipe 52, the semi-annular wall pipe 52 are sleeved on fuel cylinder 5, and the semi-annular wall pipe 52 is fixed on 2 leading flank of shell, the semi-annular wall pipe by screw II 53 52 pairs of fuel cylinders 5 play the role of axially fastening, and 3 centre position of aseptic inoculation platform is equipped with ventilation hole 19, the ventilation hole 19 upsides are equipped with fuel nipple 13, are fixed by welding manner between 19 upside aseptic inoculation platform 3 of the fuel nipple 13 and ventilation hole, The fuel nipple 13 is annular, and eight flame jet ports are uniformly equipped in the radial direction on 13 medial slope of the fuel nipple 131, direction and the 3 surface angulation of aseptic inoculation platform of 131 axis of flame jet port are 30 °, and the fuel cylinder 5 passes through Fuel hose is connect with the fuel nipple 13, and 2 leading flank centre position of the shell is equipped with ignitor switch 18, the ignitor switch 18 be electronic valve, and the ignitor switch 18 is connect by conducting wire with fuel nipple 13, and fuel nipple 13, which may be implemented, by it strikes sparks, The air that the fuel nipple 13 is blown into air blower 15 by way of heating 3 upside of aseptic inoculation platform carries out sterilization antivirus, It is operated to inoculation and creates a sterile environment.2 leading flank right end of the lower housing is equipped with battery pack 6,6 lower part of the battery pack Equipped with babinet 61, the battery pack 6 is located inside babinet 61, and 6 outer surface of the battery pack is contacted with the cooperation of 61 inner surface of babinet, 19 lower end of the ventilation hole is equipped with air blower 15, and 15 case weld of the air blower is on 19 inner wall of ventilation hole, the lower housing 2 18 right side of leading flank ignitor switch is equipped with quantity switch of air 17, and the quantity switch of air 17 passes through conducting wire and air blower 15,6 phase of battery pack Even, 15 air volume of keying and air blower of air blower 15 is controlled by quantity switch of air 17, is roused in the ventilation hole 19 Filter membrane 14 is equipped between wind turbine 15 and fuel nipple 13, the filter membrane 14 is blown into 3 upside of aseptic inoculation platform to air blower 15 Air carry out preliminary filtering.
The forward position in 13 left and right sides of fuel nipple is equipped with cover board 16,16 quantity of the cover board on the aseptic inoculation platform 3 For each one of left and right, 16 shape of the cover board is rectangle, and one end far from fuel nipple 13 is equipped with hinge 10 on the cover board 16, Described two cover boards 16 are respectively provided with two hinges 10, are connected by hinge 10 between the cover board 16 and aseptic inoculation platform 3, described It is equipped with handle I 161 close to the position of fuel nipple 13 on cover board 16, a handle I 161, institute are respectively provided on described two cover boards 16 It states the position corresponding with the cover board 16 of aseptic inoculation platform 3 and is equipped with rectangle counter sink 31, in 31 upper blind bore of the counter sink Wall is contacted with the cooperation of 16 side of the cover board.
3 lower part of aseptic inoculation platform is equipped with test tube case 4, and test tube 12 is stored in the test tube case 4, described Test tube case 4 is used for the temporary storage of test tube 12, and the test tube case 4 is the body structure of upper opening, by cover board 16 Opening can be such that test tube case 4 is connected with 3 operating space of aseptic inoculation platform, and 4 left and right sides of test tube case are respectively provided with A handle II 41, the test tube case 4 can be convenient in test tube case 4 from the 1 inside extraction of removable small handcart Test tube 12 is replaced, and test tube holding frame 11 is equipped in the test tube case 4,11 both ends of test tube holding frame are welded and fixed In test tube case 4, the test tube 12 is fixed by test tube holding frame 11, prevents toppling for test tube.
3 fuel nipple of aseptic inoculation platform, the 13 left side rearward position is equipped with homogenizer 8, and the homogenizer 8 includes hand Handle 82, hammering plate 83 and front end cap 85, the homogenizer 8 are connected by conducting wire with battery pack 6, and the hammering plate 8 is wave Shape so that the hammering plate 83 can preferably cause to shake, and generate in the solution when hammering sample homogenizing bag repeatedly The vortex of part accelerates mixing, efficiently separates the microorganism on solid sample surface, and microorganism in solution is made to be in uniform Distribution, 8 front end cap 85 of the homogenizer are full open formula glass transparent window door, facilitate observation, the homogenizer 8 right Side front end is equipped with handle 82, and the handle 82 is used for controlling the keying of full open formula glass transparent window door, 8 liang of the homogenizer The position of side bottom end is set there are four cuboid protrusion I 84, and raised I 84 centre positions of the cuboid are equipped with unthreaded hole, the nothing Position corresponding with raised I 84 unthreaded holes of the cuboid is equipped with threaded hole, the homogenizer 8 and aseptic inoculation in bacterium vaccination station 3 It is fixedly connected by screw I 81 between platform.3 fuel nipple of aseptic inoculation platform, the 13 right side rearward position is equipped with sample storage box 9, the sample storage box 9 is used for storing samples bag, and temperature sensor is equipped in the sample storage box 9, temperature controller, is added Hot device and refrigerator, the temperature sensor are connected by conducting wire with temperature controller, heater and refrigerator, and the temperature passes Sensor is used for incuding the temperature in sample storage box, and the temperature controller is stored by heater and refrigerator to control sample Temperature in case can effectively ensure that submitted sample to that need to send the microbiological specimens being further processed to laboratory True microbial status, the position of 9 two sides bottom end of sample storage box described in the temperature sensor set that there are four cuboids Protrusion II 92, raised II 92 centre positions of the cuboid are equipped with unthreaded hole, on the aseptic inoculation platform 3 with cuboid protrusion The corresponding position of II 92 unthreaded holes is equipped with threaded hole, is fixed by screw II 91 between the sample storage box 9 and aseptic inoculation platform 3 Connection.
In seeded process, the valve 54 being first turned on fuel cylinder 5 presses ignitor switch 18 so that flame jet port 131 jet flames, and make it stable burning one minute, during burning, window outward bulge that internal gas flow passes through side Go out, form a kind of inverted draft, external air is prevented to enter so that inside forms a kind of germ-free condition, then passes through air quantity Switch 17 controls air blower 15 to air is blown into inside upper shell 1, and air is entered by ventilation hole 19 in upper shell 1, then is passed through The thin-skinned curtain discharge in 1 both sides of upper shell, by the sterilization of the filtering and fuel nipple 13 of filter membrane 14, nothing is formed inside upper shell 1 Hand is passed through the window of 1 both sides of upper shell to enter inside all upper shells 1, from sample storage box 9 by bacterium area during inoculation Middle taking-up sample sack, sample sack is put into homogenizer 8, and the hammer action by hammering plate 83 keeps the microorganism of sample surfaces de- It falls, cover board 16 is opened by handle I 161, test tube 12 is taken out from test tube case 4 and carries out inoculation operation, it will after inoculation Test tube 12 after inoculation is placed on the test tube holding frame 11 in test tube case 4, prevents test tube 12 in transportational process broken, most Valve 54 is closed afterwards, closes air blower 15.
Above-mentioned, although the foregoing specific embodiments of the present invention is described with reference to the accompanying drawings, not protects model to the present invention The limitation enclosed, based on the technical solution of the present invention, those skilled in the art, which need not make the creative labor, to be made Various modifications or changes still within protection scope of the present invention.

Claims (5)

1. a kind of aseptic inoculation box, including upper shell, lower housing and the aseptic inoculation platform positioned at upper shell and lower housing joining place, It is characterized in that the upper shell upper side is transparent material, the upper shell two sides are equipped with window, and window is equipped at the window Curtain, the curtain are connect with shell at window, and the curtain is soft curtain, and the soft curtain is made of transparent plastic material, described Unthreaded hole is equipped on the upside of upper shell window, soft curtain upper end position corresponding with unthreaded hole on the upside of window is equipped with the light of same size Hole, the soft curtain upper end are fixedly connected by bolt with upper shell, and the lower housing leading flank left end is equipped with fuel cylinder, the combustion Material bottle is fixed on lower housing, and aseptic inoculation platform centre position is equipped with ventilation hole, and fuel nipple is equipped on the upside of the ventilation hole, It is fixedly connected between the fuel nipple and ventilation hole upside aseptic inoculation platform surface, the fuel nipple medial slope is equipped with flame Jet port, the fuel cylinder are connected by fuel hose with the fuel nipple, and lower housing leading flank centre position is equipped with and beats Fire switch, the ignitor switch are connected by conducting wire with fuel nipple, and the lower housing leading flank right end is equipped with battery pack, the electricity Babinet is equipped on the downside of pond group, the battery pack is fixed in babinet, and the ventilation hole lower end is equipped with air blower, outside the air blower Shell is fixedly connected with ventilation hole inner wall, and quantity switch of air is equipped on the right side of the shell leading flank ignitor switch, and the quantity switch of air is logical It crosses conducting wire with the air blower and battery pack to be connected, is equipped with filter membrane in the ventilation hole between air blower and fuel nipple, it is described Position forward at left and right sides of fuel nipple is equipped with cover board on aseptic inoculation platform, and the cover board quantity is each one of left and right, the lid Plate shape is rectangle, and the both ends far from fuel nipple are respectively provided with two hinges on described two cover boards, and the cover board connects with sterile By hinge connection between kind of platform, a handle I is respectively provided with close to the position of fuel nipple on described two cover boards, it is described sterile to connect Kind platform lower part is equipped with test tube case, and the test tube case is the body structure of upper opening, described test tube case or so Two sides are respectively provided with a handle II, and test tube holding frame, the welding of test tube holding frame both ends are equipped in the test tube case It being fixed in test tube case, test tube is fixed on the test tube holding frame, the both sides of the upper shell are fixedly connected with shoulder strap, Rearward position is equipped with homogenizer on the left of the aseptic inoculation platform fuel nipple, and the homogenizer includes hammering plate, handle and front End cap, the aseptic inoculation platform position corresponding with the cover board are equipped with rectangular counter sink, the counter sink upper blind bore Inner wall is contacted with cover board side cooperation, and the homogenizer is connected by conducting wire with battery pack, homogenizer two sides bottom The position at end is equipped with cuboid protrusion I, and raised I centre position of the cuboid is equipped with unthreaded hole, on the aseptic inoculation platform with institute It states the corresponding position of raised I unthreaded hole of cuboid and is equipped with threaded hole, fixed by screw I between the homogenizer and aseptic inoculation platform Connection, aseptic inoculation platform fuel nipple right side rearward position are equipped with sample storage box, sample storage box two sides bottom The position at end is equipped with cuboid protrusion II, and raised II centre position of the cuboid is equipped with unthreaded hole, on the aseptic inoculation platform with The corresponding position of raised II unthreaded hole of the cuboid is equipped with threaded hole, passes through spiral shell between the sample storage box and aseptic inoculation platform Nail II is fixedly connected.
2. aseptic inoculation box according to claim 1, characterized in that the homogenizer hammering plate is waveform, described equal Matter device front end cap is full open formula glass transparent window door, temperature sensor is equipped in the sample storage box, temperature controls Device, heater and refrigerator, the temperature sensor are connected by conducting wire with temperature controller, heater and refrigerator.
3. aseptic inoculation box according to claim 1, characterized in that tied between the upper shell and lower housing by buckling Structure connects, and the quantity of the buckle is front and back each two, and the upper shell upper side transparent material shell is transparent glass.
4. aseptic inoculation box according to claim 1, characterized in that the fuel cylinder upper end is equipped with valve, the fuel Bottle downside is equipped with cylindrical box, and the cylindrical box inner wall is contacted with the cooperation of fuel cylinder lower end outer surface, the combustion Expect that bottle centre position is equipped with semi-annular wall pipe, for the semi-annular wall pipe sleeve on fuel cylinder, the semi-annular wall pipe passes through screw II is fixed on lower housing leading flank.
5. aseptic inoculation box according to claim 1, characterized in that the fuel nipple and aseptic inoculation platform on the upside of ventilation hole It is fixed using welding manner between surface, the fuel nipple is annular, and flame is uniformly equipped on the fuel nipple medial slope Jet port, direction and the aseptic inoculation platform surface angulation of the flame jet port axis are 30 °, the lower housing leading flank Centre position is equipped with ignitor switch, and the ignitor switch is electronic valve, and the ignitor switch is connected by conducting wire with fuel nipple.
CN201610511103.3A 2016-07-01 2016-07-01 Aseptic inoculation box Expired - Fee Related CN106119077B (en)

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CN106119077B true CN106119077B (en) 2018-07-27

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