CN106117590A - A kind of method of modifying of leukoreduction filter film - Google Patents

A kind of method of modifying of leukoreduction filter film Download PDF

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Publication number
CN106117590A
CN106117590A CN201610473467.7A CN201610473467A CN106117590A CN 106117590 A CN106117590 A CN 106117590A CN 201610473467 A CN201610473467 A CN 201610473467A CN 106117590 A CN106117590 A CN 106117590A
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modified
mixed liquor
modifying
filter film
filter membrane
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王新平
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Guangzhou Xinkeli Biological Technology Co Ltd
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Guangzhou Xinkeli Biological Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/12—Chemical modification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/0093—Chemical modification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/76—Macromolecular material not specifically provided for in a single one of groups B01D71/08 - B01D71/74
    • B01D71/82—Macromolecular material not specifically provided for in a single one of groups B01D71/08 - B01D71/74 characterised by the presence of specified groups, e.g. introduced by chemical after-treatment
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L39/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen; Compositions of derivatives of such polymers
    • C08L39/04—Homopolymers or copolymers of monomers containing heterocyclic rings having nitrogen as ring member
    • C08L39/06—Homopolymers or copolymers of N-vinyl-pyrrolidones
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L71/00—Compositions of polyethers obtained by reactions forming an ether link in the main chain; Compositions of derivatives of such polymers
    • C08L71/02—Polyalkylene oxides
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L75/00—Compositions of polyureas or polyurethanes; Compositions of derivatives of such polymers
    • C08L75/04—Polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds

Abstract

The present invention relates to the method for modifying of a kind of leukoreduction filter film, comprise the steps: that hydrophobic resin filter membrane is placed in the first modified mixed liquor by S1 to carry out impregnating for the first time, time is 0.5～2h, temperature is 20～40 DEG C, pressure is 1～10MPa, at 50～60 DEG C, it is dried 6～10h after taking out washing, obtains the first modified filtering membrane；Described first modified filtering membrane is placed in the second modified mixed liquor and carries out impregnating for the second time by S2, and the time is 0.5～2h, and temperature is 20～40 DEG C, is dried 6～10h, obtains modified filter membrane after taking out washing at 50～60 DEG C.The modified filter membrane that the inventive method obtains has preferable wettability, stability and the compatibility, it is possible to achieve fresh blood and room temperature preserve the platelet in blood and is activated seizure leukocyte, it is achieved that to leukocyte with hematoblastic filter simultaneously, and filtration result is good.

Description

A kind of method of modifying of leukoreduction filter film
Technical field
The invention belongs to leukoreduction filter technical field, be specifically related to the method for modifying of a kind of leukoreduction filter film.
Background technology
Containing substantial amounts of leukocyte in undressed whole blood or blood component preparation, in input patient body, many can be caused Side effect, therefore filtrating leukocytes is significant.Leukoreduction filter method is by using non-woven fabrics or having intercommunication space Porous material carrys out filtrating leukocytes as filter membrane, is widely used in filtering of leukocyte.
Leukoreduction filter film is usually and is made nonwoven filtration film by the thinnest fibrous material, and preferable filter membrane should have Following characteristics: fibre diameter is ultra-fine, the aperture of filter material is little, voidage big, surface has excellent adsorptivity and wettability, simultaneously Also should have preferable blood compatibility and less expensive price, it is easy to industrialized production and there is certain mechanical performance etc.. The material being currently used for making leukoreduction filter film is broadly divided into two big classes: a class is with polyester fiber, acetate fiber as representative Organic synthetic fibers, a class is inorganic glass fiber, and what wherein polyester fiber was the most commonly used is polybutylene terephthalate Ester, what inorganic glass fiber was the most commonly used is alkali-free glass fibre.But organic synthetic fibers has nature hydrophobic, surface tension Low, it is difficult to by the feature of blood moistening, causes the rate of filtration slow, and reduce the effective surface area of filtering material contact blood, The rejection rate making leukocyte declines, additionally, organic synthetic fibers rough surface, has tiny branch, is prone to be subject to when erythrocyte passes Wound.Glass fibre has the highest surface tension, and wettability is good, and blood is fast easily by, the rate of filtration, smooth surface make red carefully Being difficult to injured when born of the same parents pass, and physical property is stable, easily realize copolymer coating, to change the quantity of electric charge, to reach absorption white thin The purpose of born of the same parents, but the processing technology of glass fibre is more complicated, relatively costly.
Now there are some researches show that platelet also can induce body to produce antiplatelet antibody.Antiplatelet antibody is one self Antibody, the histoorgan nonrecognition to self, the histoorgan of self can be attacked, thus cause damage, therefore, in order to reduce because of Platelet transfusion and the side effect that causes, people are devoted to that research is a kind of removes platelet while removing leukocyte the most expeditiously Means.
Chinese patent application 02816396.6 discloses coating and removes the filter material of leukocyte, and this filter material is in mistake Filter raw-material surface-coated by the unit from hydrophobic polymerizable monomer, from the polymerism list containing basic nitrogen-containing groups The unit of body and the coating polymer of the unit composition from the polymerizable monomer containing protic Neutral hydrophilic part, carry High to leukocyte and hematoblastic rejection rate, but rejection rate is the most not ideal enough.
Chinese patent application 201410106033.4 discloses a kind of leukoreduction filter film, including poly terephthalic acid fourth two Alcohol ester nonwoven fabric substrate and the graft modification thing being grafted in this nonwoven fabric substrate, realized by gamma-radiation irradiation Grafting, improves the leukocyte depletion rate of filter membrane, but the filter membrane obtained is high to hematoblastic transmitance, it is impossible to remove blood simultaneously Platelet.
The requirement of current 3rd generation leucocyte filter is that rejection rate reaches 99.99%.
Summary of the invention
For solve prior art exist problem, the present invention provides the method for modifying of a kind of leukoreduction filter film, by Under the conditions of certain pressure, the dipping modification in the first modified mixed liquor of hydrophobic resin filter membrane, makes the threadiness of leukocyte filter membranes State and density change, then are changed by the second modified mixed liquor dipping being made up of amidatioon modification fibroin albumen and polyurethane Crosslink after property, obtain modified filter membrane.Modified filter membrane has good wettability, stability and the compatibility, Dissolution rate in the hot water is less, and fiber surface is smooth, it is possible to achieve fresh blood (warm blood) and room temperature preserve in blood Platelet is activated, and platelet plays the effect that absorption is put up a bridge, and promotes the seizure of leukocyte, it is achieved to leukocyte and hematoblastic Filtering, erythrocyte breakage rate is low simultaneously, and filtration result is good.
The present invention provides the method for modifying of a kind of leukoreduction filter film, comprises the steps:
Hydrophobic resin filter membrane is placed in the first modified mixed liquor and carries out impregnating for the first time by S1, and the time is 0.5～2h, Temperature is 20～40 DEG C, and pressure is 1～10MPa, is dried 6～10h after taking out washing at 50～60 DEG C, obtains the first modified mistake Filter membrane；
Described first modified filtering membrane is placed in and carries out second time in the second modified mixed liquor and impregnate by S2, the time be 0.5～ 2h, temperature is 20～40 DEG C, is dried 6～10h, obtains modified filter membrane after taking out washing at 50～60 DEG C；
Described first modified mixed liquor is added to the water stirring by polyvinylpyrrolidone and fatty alcohol-polyoxyethylene ether and obtains, Mass fraction is 1～6%；Described second modified mixed liquor is modified fibroin albumen by amidatioon and polyurethane is added to the water and stirs Arriving, mass fraction is 5～10%.
Leukocyte in fresh blood can do amoeboid movement, and prior art needs cooled necessarily to the fresh blood taked Filter again after time, with the impact avoiding leukocyte to deform.Use the modified filter membrane that technique scheme obtains Can realize the leukocyte in fresh blood and hematoblastic filter, it is not necessary to blood is carried out cooling can carry out leukocyte and Hematoblastic filter, and filtration result is good.
Polyurethane is the polymer on macromolecular structure main chain containing many carbamate groups, has excellent life The thing compatibility and cohesive, the carbamate in its structure is based polar very strong, can dramatically increase polybutylene terephthalate Ester filter membrane surface tension force, reduces its hydrophobicity so that it is hydrophilic and be prone to be spontaneously wet out by water, thus improves connecing of filter membrane and blood Touch surface area, improve leukocyte and hematoblastic filter effect.Polyurethane and amidatioon are modified and are existed between silk fibroin molecular Hydrogen bond action, by certain mass than with the use of, be beneficial to keep filter membrane surface uniformity.Polyurethane molecular amount in the present invention It is 12000～18000D.
Preferably, in the described first modified mixed liquor, polyvinylpyrrolidone and the mass ratio of fatty alcohol-polyoxyethylene ether It is 2～5:1.
It is highly preferred that in the described first modified mixed liquor, polyvinylpyrrolidone and the quality of fatty alcohol-polyoxyethylene ether Ratio is 3:1.
Preferably, in described second modified mixed liquor, amidatioon modify the mass ratio of fibroin albumen and polyurethane be 0.5～ 2:1。
It is highly preferred that in the described second modified mixed liquor, amidatioon is modified the mass ratio of fibroin albumen and polyurethane and is 0.8:1。
Preferably, described amidatioon is modified the preparation of fibroin albumen and is comprised the steps: fibroin albumen is dissolved in water, makes Concentration is the solution of 10mg/mL, adds the aqueous solution of urea of 1mol/L, mixing with the volume ratio of 1:5, then uses dilute hydrochloric acid solution Regulation pH value, to 4.75, is subsequently adding 1-(3-the dimethylamino-propyl)-3-ethyl carbodiimide hydrochloride of 2 times of weight of fibroin albumen Salt, at room temperature reaction 0.5～1h, suitably instill dilute hydrochloric acid solution in course of reaction, and the pH of holding reactant liquor is 4.75, instead After should terminating, adding pH is the acetate buffer of 4.75,1-(3-the dimethylamino-propyl)-3-ethyl carbodiimide of decomposing excessive Hydrochlorate, then dialyses in deionized water by reactant liquor, lyophilization, obtains amidatioon and modifies fibroin albumen.Amide Changing and modify the amidated products that fibroin albumen is fibroin albumen side chain carboxyl group, the preparation method provided by the present invention is changed certainly By the quantity of carboxyl, reach to change the charge on fibroin albumen surface, improved absorption property.
Preferably, the mass fraction of the described first modified mixed liquor is 3～5%, it is highly preferred that the described first modified mixing The mass fraction of liquid is 4%；The mass fraction of the described second modified mixed liquor is 6～9%, it is highly preferred that described second is modified The mass fraction of mixed liquor is 8%.
Preferably, the temperature of dipping of described first time is 25～40 DEG C, and pressure is 2～5MPa.It is highly preferred that described first The temperature of secondary dipping is 30 DEG C, and pressure is 4MPa.The pressure of second time dipping is normal pressure.
Preferably, described hydrophobic resin filter membrane is selected from polybutylene terephthalate (PBT) filter membrane.
Additionally, the present invention also provides for a kind of modified filter membrane, use the method for modifying system of above-mentioned leukoreduction filter film ?.The average pore diameter of modified filter membrane is 6～12 μm, less than average pore diameter before modified.
Compared with prior art, the beneficial effect comprise that the present invention passes through hydrophobicity under the conditions of certain pressure Resin filter film impregnates modified in the first modified mixed liquor, makes the fiber condition of leukocyte filter membranes and density change, The second modified mixed liquor by being made up of amidatioon modification fibroin albumen and polyurethane impregnates modified crosslinking again, it is achieved The modification of filter membrane.The filter membrane obtained by two step modifications has good wettability, stability and the compatibility, in the hot water Dissolution rate less, and fiber surface is smooth, it is possible to achieve the platelet that fresh blood and room temperature preserve in blood is activated, blood Platelet plays the effect that absorption is put up a bridge, and promotes the seizure of leukocyte, it is achieved to leukocyte with hematoblastic filter, erythrocyte simultaneously Breakage rate is low, and filtration result is good.
Detailed description of the invention
Further describe the present invention below by way of specific embodiment, but the present invention is not limited only to following example.
The modified mixed liquor of embodiment one first
First modified mixed liquor is added to the water with the mass ratio of 3:1 by polyvinylpyrrolidone and fatty alcohol-polyoxyethylene ether Stirring obtains, and mass fraction is 4%.
The modified mixed liquor of embodiment 2 first
First modified mixed liquor is added to the water with the mass ratio of 4:1 by polyvinylpyrrolidone and fatty alcohol-polyoxyethylene ether Stirring obtains, and mass fraction is 5%.
The modified mixed liquor of comparative example one first
First modified mixed liquor is added to the water with the mass ratio of 1:1 by polyvinylpyrrolidone and fatty alcohol-polyoxyethylene ether Stirring obtains, and mass fraction is 4%.
The modified mixed liquor of embodiment 3 second
Second modified mixed liquor is modified fibroin albumen by amidatioon and polyurethane is added to the water stirring with the mass ratio of 0.8:1 Obtaining, mass fraction is 8%.
Amidatioon is modified the preparation of fibroin albumen and is comprised the steps: fibroin albumen is dissolved in water, and making concentration is 10mg/ The solution of mL, adds the aqueous solution of urea of 1mol/L, mixing, then regulates pH value extremely with dilute hydrochloric acid solution with the volume ratio of 1:5 4.75, it is subsequently adding 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 2 times of weight of fibroin albumen, in room temperature Lower reaction 1h, suitably instills dilute hydrochloric acid solution in course of reaction, keeps the pH of reactant liquor 4.75, after reaction terminates, adds PH is the acetate buffer of 4.75,1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of decomposing excessive, then will Reactant liquor is dialysed in deionized water, lyophilization, obtains amidatioon and modifies fibroin albumen.
The modified mixed liquor of comparative example 2 second
Second modified mixed liquor is added to the water stirring by fibroin albumen and polyurethane with the mass ratio of 0.8:1 and obtains, quality Mark is 8%.
The modification of embodiment four leukoreduction filter film
Polybutylene terephthalate (PBT) filter membrane is placed in the first modified mixed liquor that embodiment one prepares and carries out the Single-steeping, the time is 1h, and temperature is 30 DEG C, and pressure is 4MPa, is dried 8h, obtains first and change after taking out water washing at 60 DEG C Property filter membrane；Described first modified filtering membrane is placed in the second modified mixed liquor that embodiment three prepares and carries out soaking for the second time Stain, the time is 1h, and temperature is 30 DEG C, is dried 8h, obtains modified filter membrane after taking out water washing at 60 DEG C.
The modification of embodiment five leukoreduction filter film
Polybutylene terephthalate (PBT) filter membrane is placed in the first modified mixed liquor that embodiment two prepares and carries out the Single-steeping, the time is 1.5h, and temperature is 30 DEG C, and pressure is 2MPa, is dried 8h, obtains first after taking out water washing at 60 DEG C Modified filtering membrane；Described first modified filtering membrane is placed in the second modified mixed liquor that embodiment three prepares and carries out soaking for the second time Stain, the time is 1h, and temperature is 30 DEG C, is dried 8h, obtains modified filter membrane after taking out water washing at 60 DEG C.
The modification of comparative example three leukoreduction filter film
Polybutylene terephthalate (PBT) filter membrane is placed in the first modified mixed liquor that embodiment one prepares and carries out the Single-steeping, the time is 1h, and temperature is 30 DEG C, and pressure is normal pressure, is dried 8h, obtains first and change after taking out water washing at 60 DEG C Property filter membrane；Described first modified filtering membrane is placed in the second modified mixed liquor that embodiment three prepares and carries out soaking for the second time Stain, the time is 1h, and temperature is 30 DEG C, is dried 8h, obtains modified filter membrane after taking out water washing at 60 DEG C.
This comparative example is with the difference of embodiment four: the pressure of dipping is become normal pressure from 4MPa for the first time.
The modification of comparative example four leukoreduction filter film
Polybutylene terephthalate (PBT) filter membrane is placed in the first modified mixed liquor that comparative example one prepares and carries out the Single-steeping, the time is 1h, and temperature is 30 DEG C, and pressure is 4MPa, is dried 8h, obtains first and change after taking out water washing at 60 DEG C Property filter membrane；Described first modified filtering membrane is placed in the second modified mixed liquor that embodiment three prepares and carries out soaking for the second time Stain, the time is 1h, and temperature is 30 DEG C, is dried 8h, obtains modified filter membrane after taking out water washing at 60 DEG C.
This comparative example is with the difference of embodiment four: polyvinylpyrrolidone and poly alkyl alcohol in the first modified mixed liquor The mass ratio of oxygen vinyl Ether is become 1:1 from 3:1.
The modification of comparative example five leukoreduction filter film
It is placed in polybutylene terephthalate (PBT) filter membrane in the second modified mixed liquor that embodiment three prepares Row dipping, the time is 1h, and temperature is 30 DEG C, is dried 8h, obtains modified filter membrane after taking out water washing at 60 DEG C.
This comparative example is with the difference of embodiment four: lack the step of dipping for the first time.
The rate that the washes out test of the filter membrane after test example is modified
The modified filtration respectively embodiment four, embodiment five, comparative example three, comparative example four and comparative example five prepared Film carries out washing out rate test, particularly as follows: take 15g polybutylene terephthalate (PBT) filter membrane to be after modified placed in 200mL's In container, addition normal saline solution, as filling liquid, then carries out γ ray sterilization (illuminated line amount 25kGy).In order in reality Confirm washings within the temperature range of considering during the keeping medical instruments of border, at 4 DEG C, after preserving 24h at 25 DEG C, preserve 24h again. Observe the outward appearance of filling liquid after preserving, and it is maximum to use ultraviolet spectrophotometer to measure the filling liquid when wavelength is 220～350nm Absorbance.
The rate that the washes out result of the test of table 1 filtering material of the present invention
Filling liquid outward appearance Maximum absorbance
Embodiment four Water white transparency 0.043
Embodiment five Water white transparency 0.046
Comparative example three Water white transparency 0.045
Comparative example four Water white transparency 0.052
Comparative example five Water white transparency 0.048
As known from Table 1, the modified filter membrane that the present invention prepares has preferable stability, washes out degree low, meets Related request.
The filtration result test of the filter membrane that test example two is modified
Filter velocity test: be respectively adopted containing embodiment four, embodiment five, comparative example three, comparative example four and comparative example five The filter of the modified filter membrane prepared, on-line filtration 400mL whole blood, record Whole Blood Filtration required time, and it is right to test it Leukocyte and hematoblastic filtration result, use blood-counter system test to filter the quantity of leucocyte in forward and backward whole blood；Record 400mL Whole Blood Filtration proleukocyte and hematoblastic quantity are respectively 2.5 × 109Individual and 7.2 × 1010Individual.
The method of testing wherein remaining leukocyte count is: in the blood plasma sampling after filtering to the test tube of polyethylene, with After the leukocyte dyeing that acridine orange liquid will spill, it is measured with fluorescence microscope.The white blood cell concentration of test is multiplied by recovery The liquid measure of blood, records residue leukocyte count contained in reclaiming bag.Referring concurrently to industry standard YY 0,329 2009 (once Property use filter for removing white blood cell) in method, degree of injury erythrocytic in reclaiming bag is tested.Test result is the most such as Shown in table 2, table 3 and table 4.
Leukocyte (platelet) rejection rate (%)=L0-L1/L0× 100, wherein L0For unit volume whole blood containing before filtering Some leukocyte (platelet) quantity, L1For leukocyte (platelet) quantity contained in unit volume whole blood after filtering.
The Whole Blood Filtration time of the filter membrane that table 2 is modified
Filtration time (min)
Embodiment four 14.5
Embodiment five 15.2
Comparative example three 18.0
Comparative example four 16.8
Comparative example five 21.6
As known from Table 2, the modified filter membrane that the embodiment of the present invention obtains filters 400mL whole blood and has only to about 15min, Less than 16min, and comparative example three, comparative example four and comparative example five prepare the filtration time of modified filter membrane all at 16min Above, especially comparative example five prepares the filtration time of modified filter membrane up to 21.6min.
The modified filter membrane of table 3 is to leukocyte and hematoblastic filtration result
Residue leukocyte count Leukocyte depletion rate Residue platelet count Platelet rejection rate
Embodiment four 4.2×104 99.999% 4.2×109 94.167%
Embodiment five 4.3×104 99.999% 4.5×109 93.750%
Comparative example three 6.2×105 99.975% 9.7×109 86.528%
Comparative example four 7.4×105 99.970% 8.1×109 88.750%
Comparative example five 2.1×106 99.916% 1.3×1010 81.944%
As known from Table 3, leukocyte in whole blood and platelet are had excellent by the modified filter membrane that the embodiment of the present invention obtains Different filtration result, has exceeded the requirement of the 3rd generation leucocyte filter, hence it is evident that be better than comparative example three, comparative example four and comparative example The five modified filter membranes obtained.The modified filter membrane best results wherein prepared with embodiment four, its filter to leukocyte Except rate is up to 99.999%, hematoblastic rejection rate is reached 94.167%.
The modified filter membrane of table 4 is to erythrocytic damage measure
Free hemoglobin content (mg/L)
Embodiment four 79.5
Embodiment five 81.6
Comparative example three 93.7
Comparative example four 84.5
Comparative example five 81.5
As known from Table 4, the modified filter membrane that the embodiment of the present invention obtains is little to degree of injury erythrocytic in whole blood, phase Capacitive is good, meets related request.
In sum, the modified filter membrane that the method that the present invention provides obtains filters effect at raising leukocyte and platelet While rate, the compatibility to blood the most substantially changes, and stability and the compatibility are good.
Below it is only the preferred embodiment of the present invention, it is noted that it is right that above-mentioned preferred implementation is not construed as The restriction of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For the art For those of ordinary skill, without departing from the spirit and scope of the present invention, it is also possible to make some improvements and modifications, these change Enter and retouch and also should be regarded as protection scope of the present invention.

Claims (10)

1. the method for modifying of a leukoreduction filter film, it is characterised in that: comprise the steps:
Hydrophobic resin filter membrane is placed in the first modified mixed liquor and carries out impregnating for the first time by S1, and the time is 0.5～2h, temperature Being 20～40 DEG C, pressure is 1～10MPa, is dried 6～10h after taking out washing at 50～60 DEG C, obtains the first modified filtration Film；
Described first modified filtering membrane is placed in the second modified mixed liquor and carries out impregnating for the second time by S2, and the time is 0.5～2h, temperature Degree is 20～40 DEG C, is dried 6～10h, obtains modified filter membrane after taking out washing at 50～60 DEG C；
Described first modified mixed liquor is added to the water stirring by polyvinylpyrrolidone and fatty alcohol-polyoxyethylene ether and obtains, quality Mark is 1～6%；Described second modified mixed liquor is modified fibroin albumen by amidatioon and polyurethane is added to the water stirring and obtains, Mass fraction is 5～10%.
The method of modifying of leukoreduction filter film the most according to claim 1, it is characterised in that: the described first modified mixed liquor In, the mass ratio of polyvinylpyrrolidone and fatty alcohol-polyoxyethylene ether is 2～5:1.
The method of modifying of leukoreduction filter film the most according to claim 2, it is characterised in that: the described first modified mixed liquor In, the mass ratio of polyvinylpyrrolidone and fatty alcohol-polyoxyethylene ether is 3:1.
The method of modifying of leukoreduction filter film the most according to claim 1, it is characterised in that: the described second modified mixed liquor In, it is 0.5～2:1 that the mass ratio of fibroin albumen and polyurethane is modified in amidatioon.
The method of modifying of leukoreduction filter film the most according to claim 4, it is characterised in that: the described second modified mixed liquor In, it is 0.8:1 that the mass ratio of fibroin albumen and polyurethane is modified in amidatioon.
The method of modifying of leukoreduction filter film the most according to claim 1, it is characterised in that: fibroin is modified in described amidatioon The preparation of albumen comprises the steps: fibroin albumen is dissolved in water, makes the solution that concentration is 10mg/mL, with the volume ratio of 1:5 Add the aqueous solution of urea of 1mol/L, mixing, then with dilute hydrochloric acid solution regulation pH value to 4.75, be subsequently adding fibroin albumen 2 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of times weight, at room temperature reaction 0.5～1h, was reacting Suitably instilling dilute hydrochloric acid solution in journey, keep the pH of reactant liquor 4.75, after reaction terminates, adding pH is the acetate buffer of 4.75 Liquid, 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of decomposing excessive, then by reactant liquor in deionized water Dialyse, lyophilization, obtain amidatioon and modify fibroin albumen.
The method of modifying of leukoreduction filter film the most according to claim 1, it is characterised in that: the described first modified mixed liquor Mass fraction be 3～5%；The mass fraction of the described second modified mixed liquor is 6～9%.
The method of modifying of leukoreduction filter film the most according to claim 1, it is characterised in that: the temperature of dipping of described first time Degree is 25～40 DEG C, and pressure is 2～5MPa.
The method of modifying of leukoreduction filter film the most according to claim 1, it is characterised in that: described hydrophobic resin filters Film is selected from polybutylene terephthalate (PBT) filter membrane.
10. a modified filter membrane, it is characterised in that: use the leukoreduction filter film described in any one of claim 1 to 9 Method of modifying prepare.
CN201610473467.7A 2016-06-22 2016-06-22 A kind of method of modifying of leukoreduction filter film Withdrawn CN106117590A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107138058A (en) * 2017-07-11 2017-09-08 广州达济医学科技有限公司 A kind of method of modifying of leukoreduction filter film
CN108976450A (en) * 2018-04-23 2018-12-11 安徽工程大学 Based on wax, growth prepares the method for super-hydrophobic fibroin protein film, a kind of super-hydrophobic fibroin protein film certainly

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547576A (en) * 1992-07-06 1996-08-20 Terumo Kabushiki Kaisha Pathogenic substance removing material and a blood filter containing the material
CN1177620C (en) * 1997-08-28 2004-12-01 旭医学株式会社 Leukocyte-removing filter material
CN1253233C (en) * 2001-01-29 2006-04-26 旭医学株式会社 Fiter for processing blood and producing method for same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547576A (en) * 1992-07-06 1996-08-20 Terumo Kabushiki Kaisha Pathogenic substance removing material and a blood filter containing the material
CN1177620C (en) * 1997-08-28 2004-12-01 旭医学株式会社 Leukocyte-removing filter material
CN1253233C (en) * 2001-01-29 2006-04-26 旭医学株式会社 Fiter for processing blood and producing method for same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107138058A (en) * 2017-07-11 2017-09-08 广州达济医学科技有限公司 A kind of method of modifying of leukoreduction filter film
CN107138058B (en) * 2017-07-11 2018-04-06 广州达济医学科技有限公司 A kind of method of modifying of leukoreduction filter film
CN108976450A (en) * 2018-04-23 2018-12-11 安徽工程大学 Based on wax, growth prepares the method for super-hydrophobic fibroin protein film, a kind of super-hydrophobic fibroin protein film certainly

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Application publication date: 20161116