CN106117044B - The method that alltrans aliphatic acid with anti-tumor activity is detached from antarctic krill oil - Google Patents
The method that alltrans aliphatic acid with anti-tumor activity is detached from antarctic krill oil Download PDFInfo
- Publication number
- CN106117044B CN106117044B CN201610421280.2A CN201610421280A CN106117044B CN 106117044 B CN106117044 B CN 106117044B CN 201610421280 A CN201610421280 A CN 201610421280A CN 106117044 B CN106117044 B CN 106117044B
- Authority
- CN
- China
- Prior art keywords
- alltrans
- dha
- epa
- fraction
- antarctic krill
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
- C07C51/44—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation by distillation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
- A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/48—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C57/00—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms
- C07C57/02—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms with only carbon-to-carbon double bonds as unsaturation
- C07C57/03—Monocarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C1/00—Preparation of fatty acids from fats, fatty oils, or waxes; Refining the fatty acids
- C11C1/08—Refining
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/09—Geometrical isomers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The method that the present invention provides a kind of to detach alltrans aliphatic acid with anti-tumor activity from antarctic krill oil.Antarctic krill oil is coupled preparative high-performance liquid chromatographic with silica gel column chromatography to isolate and purify, adjunct antineoplastic Activity determination determines active ingredient, molecular weight, structure composition and the configuration for determining active ingredient respectively through high-resolution electrospray ionization mass spectrometry, nuclear magnetic resonance and laser raman scattering spectrum, it is alltrans EPA and alltrans DHA to identify active ingredient.Compare krill, commercially available deep sea fish oil, Amur sturgeon liver EPA and DHA to find the half lethal concentration of kinds of tumor cells, the alltrans EPA and alltrans DHA in antarctic krill oil source have the function of excellent antitumor cell growth, and there is the development prospect of potential antitumor drug candidate without obvious inhibiting effect to normal control cells.
Description
Technical field
The method that the present invention relates to a kind of to detach natural E types aliphatic acid with anti-tumor activity from antarctic krill oil,
Identified alltrans EPA and DHA is to human breast carcinoma, hepatocellular carcinoma, early young grain leukaemia, chronic myelocytic leukemia, tissue
Cell lymphoma etc. has the effect of significant antitumor cell growth.
Background technology
In in the past few decades, the incidence of tumour is in ascendant trend year by year, even if there is being constantly progressive for medical technology,
Tumour is still the big cause of disease of peacetime human death.Researcher studies the generation and development of tumour from different directions,
And the three big therapies based on radiotherapy (radiotherapy), chemotherapy (chemotherapy) and operation therapy (operation) are developed,
But there is different degrees of drawback in three big therapies.Radiotherapy there are significant side effect, such as loss of appetite, alopecia, it is drowsiness, white carefully
Born of the same parents reduce, some patientss also with bleeding situations such as；Operative treatment is preferable to tumorigenic early period or prometaphase effect, but swollen
After tumor is spread, the effect of operative treatment is just deteriorated, and operative treatment is all larger to the psychology and physiological load of patient, has
A little patients can cause sequelae or complication because of operation, and operative treatment can only be directed to solid tumor, to non-physical knurl such as blood
Cancer etc. is helpless.The drug of chemotherapy is relatively more, gradually forms the series production such as synthetic drug, Chinese medicine or natural products, bio-pharmaceutical
Product, but there are the drawbacks such as poor specificity for many antitumor drugs, after some drugs enter patient's body, killing tumor cell it is same
When also largely kill patient's body normal cell, lead to a series of side effects, such as alopecia, vomiting, nausea and loss of appetite,
Therefore good drug of developing a kind of safe, Small side effects, antitumous effect be people there is an urgent need to wherein how unsaturateds
The research and development of aliphatic acid once got more and more people's extensive concerning.
Aliphatic acid is the main component of fat, phosphatide and glycolipid, is the material base of life.Whether contain according in its carbochain
There are unsaturated bond or double bond and is divided into saturated fatty acid and unsaturated fatty acid, the aliphatic acid quilt containing more than two unsaturated bonds
Referred to as polyunsaturated fatty acid, as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contain respectively there are five and six
Double bond.According to its configuration aliphatic acid have again cis- (Z-type) and trans- (E types) it is not, the EPA in common deep sea fish oil and DHA
It is Z-type, it is considered to be the ω -3 races aliphatic acid of great nutritive value and physiological activity.Have studies have shown that Z-EPA and Z-
DHA has enhancing human immunity, antiallergy and promotes the important function such as intracellular fatty acid metabolism, and DHA is to infant god
Significant role is also played through systematic growth.Since nineteen seventies, researcher has found that Z-EPA and Z-DHA has
Inhibit the ability of kinds of tumor cells growth, the half lethal concentration of young grain acute leukemia cells system HL60 such as early to people
(IC50) it is respectively 85 μM and 70 μM, to the IC of human breast carcinoma cell lines MCF-750Respectively 85 ± 5 μM and 65 ± 5 μM；They
Main function mechanism be inducing apoptosis of tumour cell or cause growth of tumour cell Cycle Arrest.At this stage due to Z-EPA and
Z-DHA just can must generate lethal effect to kinds of tumor cells in higher concentrations, its application is caused to be very limited.
Trans- (E types) aliphatic acid has naturally occurring and artificial manufacture two types, such as contains 4- in milk from cows and goats milk product
9% E type fatty acid or esters, and hydrogenated oil and fat is then a kind of unsaturated fatty acid that hydrogenation modification generation is carried out to vegetable oil, is contained
There is the E type aliphatic acid of 14.2-34.3%；There are an E type carbon-carbon double bonds to be also categorized as E type fat for conjugated linoleic acid (CLA)
Acid.There are larger disputes for the biological function of this kind of aliphatic acid, especially it is carcinogenic and it is for cancer two seem opposition in terms of.It grinds
Study carefully and shows that CLA can also have research to think that it can accelerate the pernicious process of breast cancer, separately have research to think with adjuvant treatment of breast cancer
Non-correlation between the two fails to form final conclusion so far.To the research of E type aliphatic acid mainly based on E- oleic acid, research is thought
The high intake of E- oleic acid can increase the risk of angiocardiopathy, especially coronary heart disease, but to early growth and development, II type glycosuria
The correlation of the diseases such as disease, hypertension, cancer and trans fats, is able to verify that currently without positive evidence.E types EPA and DHA make
For the isomers of Z-type EPA and DHA, not yet have been reported that so far.
Krill grease is rich in polyunsaturated fat, especially omega-3 unsaturated fatty acid, wherein EPA and DHA content
Up to 20% or more.It is generally believed that the EPA and DHA in krill are Z-types, there is anti-oxidant, anti-aging, antitumor
The effects that, but be actually mostly to analogize, lack experimental basis.The present invention is using solvent extraction, silica gel column chromatography, preparation efficient liquid phase
The methods of chromatography isolates and purifies krill grease, passes through high-resolution electrospray ionization mass spectrometry (HRESIMS), nuclear-magnetism
Two single components of the technical appraisement such as resonance, Raman spectrum are E type EPA and DHA, and antitumor activity is better than the city based on Z-type
Fish oil EPA and DHA are sold, new clue is provided for the exploitation of new type antineoplastic medicine.
Invention content
The purpose of the present invention is to provide one kind to have good antitumor action, and safe, Small side effects anti-swollen
Tumor agent-alltrans polyunsaturated fatty acid, E types EPA and DHA.With mixed extractant solvent, silica gel column chromatography, preparative liquid chromatography
Active principle with anti-tumor activity in krill grease is isolated and purified, gas chromatography-mass spectrography, liquid phase color are passed through
The structure of the Instrumental Analysis active ingredients such as spectrum-mass spectrometry, high-resolution electrospray ionization mass spectrometry, nuclear magnetic resonance, Raman spectrum, and
The influence that they grow kinds of tumor cells is investigated, and is compared with commercially available fish oil EPA and DHA and sturgeon liver EPA and DHA
Compared with.
The present invention provides a kind of alltrans EPA with anti-tumor activity or alltrans DHA, divides from antarctic krill oil
From obtaining.EPA and DHA in common deep sea fish oil are Z-types, isomers of the E type EPA and DHA as Z-type EPA and DHA,
So far there has been no antitumor activity reports.
The present invention also provides natural alltrans EPA or alltrans with anti-tumor activity are detached from antarctic krill oil
The method of DHA comprising following steps：
(1) antarctic krill oil is added into silicagel column and carries out column chromatography for separation, collect eluent, removed solvent, obtain
Fraction I and fraction II, fraction I are yellow oil, and fraction II is cerise grease；
(2) dilution in acetonitrile of fraction II, is detached with high performance preparative liquid chromatography method, with the mixed solvent of acetonitrile and water
System is that mobile phase is eluted, and collects elution gained different component, removes solvent, obtain compositionⅱ -1 successively to II -5,
For colourless or yellowish oily mater, wherein compositionⅱ -2 is alltrans EPA, and compositionⅱ -4 is alltrans DHA.
Further, in the column chromatography for separation described in step (1), the eluent is that volume ratio is 7:3-9:1
N-hexane and ether mixed liquor, the acetic acid that addition percent by volume is 1-5% in mixed liquor.
Further, the grain size of the silica gel described in step (1) is 300~400 mesh, the quality of silica gel and antarctic krill oil
Than being 30~100:1, preferably 50~80:1, more preferably 60~70:1, chromatographic column ratio of height to diameter is 10~16:1.
Further, the chromatographic column filler described in step (2) is reverse phase C18, 10 μm of grain size, the elution program of mobile phase
It is as follows successively：Between eluting 0-10min, eluted with 62-68% acetonitriles；Between eluting 10-20min, washed with 67-73% acetonitriles
It is de-；It between eluting 20-60min, is eluted with 72-78% acetonitriles, the elution program of preferred mobile phase is as follows successively：Elute 0-
Between 10min, eluted with 65% acetonitrile；Between eluting 10-20min, eluted with 70% acetonitrile；Between eluting 20-60min, use
75% acetonitrile elutes.
In above-mentioned separation method, antarctic krill oil loading is eluted to silicagel column, and using eluent, collects elution
Liquid, the volume per fraction is the 1/20~1/40 of chromatographic column retention volume, preferably 1/30, while being detected with thin-layer chromatography method
The substance of opposed polarity contained in gained eluent merges the eluent with identical polar fraction after testing, in temperature 20-
40 DEG C, rotary evaporation removes solvent under conditions of pressure is -0.08~-0.1Mpa, respectively obtains fraction I and fraction II, fraction I
For yellow oil, fraction II is cerise grease.It is stronger anti-swollen that antitumor activity testing result shows that fraction II has
Tumor activity, using the further separate fraction of preparative high-performance liquid chromatographic column II.
In above-mentioned separation method, the isolated fraction II of silica gel column chromatography is further with reversed C18Efficiently prepare liquid phase
Chromatography is detached, and the mixed solvent with acetonitrile and water is mobile phase, and the flow velocity of mobile phase is without being particularly limited to, this field
Technical staff can select flow rate of mobile phase appropriate according to this field routine techniques, can preferably 10~20mL/min, it is more excellent
Select 15mL/min.Light absorption value at ultraviolet detection 210nm carries out Fractional Collections according to appearance time, in 20-40 DEG C of temperature,
Rotation additional issue recycling design, obtains compositionⅱ -1 to compositionⅱ -5 successively under conditions of pressure is -0.08~-0.1Mpa.It is anti-swollen
Tumor activity testing result shows that compositionⅱ -2 and II -4 has stronger antitumor activity.Compositionⅱ -2 and compositionⅱ -4 are used
HRESIMS、1H-NMR、13C-NMR, laser raman scattering spectrum etc. carry out Structural Identification, determine that compositionⅱ -2 is alltrans EPA,
Compositionⅱ -4 is alltrans DHA.
In the present invention, the antarctic krill oil is prepared as follows to obtain：Krill freezes shrimp or euphausia superba powder
For raw material, with mixed solvent (n-hexane：Ethyl alcohol=10:1, v/v) 2h is stirred at room temperature, by organic solvent mutually through rotary evaporation
It can be obtained euphausia superba sauce after concentration.In order to obtain more free EPA and DHA, enzymatic isolation method can be used to hydrolyze South Pole phosphorus
Shrimp sauce fat, specific enzymatic hydrolysis condition are：1.0g antarctic krill oils, 1.0g deionized waters and 20mg immobilized lipases (Novozym
435) it is placed in 15mL centrifuge tubes, is then reacted under the conditions of being protected from light in 30 DEG C of shaking tables 24 hours, use chlorine after reaction
Imitative extracting enzymolysis liquid, then isolated and purified.Antarctic krill oil can also use commercially available product.
The present invention also provides alltrans EPA described above or alltrans DHA application in preparations of anti-tumor drugs.It is excellent
Selection of land, the tumour are human breast carcinoma, hepatocellular carcinoma, early young grain leukaemia, chronic myelocytic leukemia, histiocytic leaching
Bar tumor or prostate cancer.
The present invention also provides including above-mentioned alltrans EPA and pharmaceutically acceptable auxiliary material, or including above-mentioned alltrans DHA
With the pharmaceutical composition of pharmaceutically acceptable auxiliary material.Preferably, described pharmaceutical composition is granule, tablet or capsule.
The invention has the advantages that：
The present invention is isolated with anti-from antarctic krill oil using the method for silica gel column chromatography coupling preparative liquid chromatography
The alltrans EPA (E-EPA) or alltrans DHA (E-DHA) of tumor promotion.EPA and DHA in common deep sea fish oil are Z-types
, isomers of the E type EPA and DHA as Z-type EPA and DHA not yet has been reported that so far.The E-EPA of krill and E-DHA pairs
There is kinds of tumor cells excellent killing ability, effect to be better than commercially available fish oil and the EPA and DHA in sturgeon liver oil source,
And there are the potentiality of exploitation new type antineoplastic medicine without apparent growth inhibition to normal liver cell HL7702.
Description of the drawings
Fig. 1 is the thin-layer chromatogram for indicating opposed polarity fraction；
Fig. 2 is the preparative high-performance liquid chromatographic figure for indicating silica gel column chromatography fraction II；
Fig. 3 is the growth inhibition curve for indicating to prepare -2 couples of MCF-7 of compositionⅱ；
Fig. 4 is the growth inhibition curve for indicating to prepare -4 couples of MCF-7 of compositionⅱ；
Fig. 5 is the mass spectrogram for indicating compositionⅱ -2 (EPA)；
Fig. 6 is the mass spectrogram for indicating compositionⅱ -4 (DHA)；
Fig. 7 indicates compositionⅱ -2 (EPA)1H-NMR schemes；
Fig. 8 indicates compositionⅱ -4 (DHA)1H-NMR schemes；
Fig. 9 indicates compositionⅱ -2 (EPA)13C-NMR schemes；
Figure 10 indicates compositionⅱ -4 (DHA)13C-NMR schemes；
Figure 11 is the laser raman scattering spectrogram for indicating krill and commercially available fish oil EPA；
Figure 12 is the laser raman scattering spectrogram for indicating krill and commercially available fish oil DHA.
Specific implementation mode
Following non-limiting embodiments can make those skilled in the art be more fully understood the present invention, but not with
Any mode limits the present invention.In following embodiments, unless otherwise specified, used experimental method is conventional method, institute
It can be bought from biological or chemical company with material, reagent etc..
Following experimental technique and analysis method involved in the specific implementation mode of the present invention：
1. cell culture
Human breast carcinoma cell lines MCF-7 and MDA-MB-231, Bel7402 SMMC-7721, the early young grain of people are acute white
Before blood disease cell line HL60, human chronic polymorpho nuclear leukemia cells system K562, human tissue cell's property lymphoma cell line U937, people
Row gland cell system PC-3 and people's normal liver tissue cell HL7702 is purchased from Cell Bank of Chinese Academy of Sciences, wherein MCF-7 and
The used culture mediums of MDA-MB-231 are DMEM high glucose mediums (Hyclone), and the used culture medium of remaining cell is RPMI-
1640 culture mediums (Hyclone) add 10%FBS (Hyclone) and 1% Pen .- Strep mixed liquor in incubation
(Hyclone).All cells are in 25cm2It is cultivated in culture bottle.Above-mentioned cell is cultivated two days later in culture bottle, attached cell
Respective handling is carried out respectively with suspension cell：A certain amount of trypsin solution is added in attached cell after PBS buffer solution is cleaned, so
After be added corresponding culture medium gently blow and beat repeatedly to cell disperse, use cell counting board calculate cell number；Suspension cell passes through
Remove supernatant after 800r/min centrifugations, washed twice using PBS buffer solution and be resuspended again with corresponding culture medium, then uses thin
Born of the same parents' tally counts.96 orifice plates are added in above-mentioned cell, the cell number for controlling each hole is 3000, the culture volume in each hole
For 200 μ L, 96 orifice plates are put into cell incubator and are incubated 24 hours.
2. antitumor activity detects
Influence of the substance isolated and purified with mtt assay or the detection of CCK-8 kits to different growth of tumour cell, with determination
Its antitumor activity.The detection of attached cell uses mtt assay, suspension cell to use CCK-8 kits.Test substance is first dissolved
It is detected again after DMSO, as a contrast with the culture medium containing 0.5%DMSO.First determinand will be added according to concentration appropriate
96 orifice plates of matter or comparison liquid are placed in cell incubator and are incubated 16-18 hours, and 20 μ L MTT or CCK-8 (Kai Ji) are added to each hole
Reagent, then light absorption value is detected respectively under 570nm (MTT)/450nm (CCK-8) and 630nm after being incubated 4 hours.Cell survival rate
It calculates as follows：
Cell survival rate (mtt assay, %)=[experimental group light absorption value (A570-A630) average value/control group light absorption value (A570-
A630) average value] * 100
Cell survival rate (CCK-8 methods, %)=[experimental group light absorption value (A450-A630) average value/control group light absorption value
(A450-A630) average value] * 100
Half lethal concentration IC50Calculating using improvement karber's method formula：
lg(IC50)=Xm-I*(P-(3-Pm-Pn)/4)
Wherein XmFor lg (maximum drug concentration), I is lg (maximum drug concentration/time high drug concentration), and P is that experimental group is thin
The sum of born of the same parents' survival rate, PmFor maximum cell survival rate, PnFor the smallest cell survival rate.
3. the extraction of antarctic krill oil：
Shrimp or euphausia superba powder are frozen as raw material, with mixed solvent (n-hexane using krill first：Ethyl alcohol=10:1, v/
V) 2h is stirred at room temperature, extracts shrimp sauce in raw material, organic solvent mutually be can be obtained into krill after rotary evaporation concentrates
Shrimp sauce.Similarly, with mixed solvent grease is extracted from sturgeon fish liver.
The separation of various components in 1 antarctic krill oil of embodiment
(1) preparation of silicagel column：It is 3.0cm that 300-400 mesh silica gel wet methods after 300g is activated, which are packed into internal diameter, a length of
In the glass splitter of 35.0cm, balanced with n-hexane.
(2) euphausia superba sauce 5g is taken, is added and carries out column chromatography for separation into ready silicagel column, use eluent successively
A (n-hexanes：Ether：Acetic acid=80:20:1.5, v/v/v) with eluent B (chloroforms：Methanol：Water=60:30:5, v/v/v) into
Row elution, per fraction be 10mL, through thin-layer chromatography detect eluent in contained substance opposed polarity, as shown in Figure 1, in figure with
Antarctic krill oil as a contrast, and number i, ii and iii correspond to respectively triglycerides or polarity close to triglycerides substance,
Oleic acid or polarity are close to the substance of oleic acid, phospholipid substance.Merge the fraction with identical polar according to thin-layer chromatography result,
Finally be obtained 4 groups of fractions, wherein fraction I is afforded with fraction II through eluent A, fraction III and fraction IV be by
Eluent B is afforded, and the total volume for collecting four kinds of fractions is respectively 510,420,285 and 225mL, by each fraction through rotation
It is concentrated by evaporation spare, the quality that four kinds of fractions are obtained after vacuum rotary steam concentrates is respectively 2.78,1.12,0.59 and 0.17g.Its
In, fraction I is yellow oily substance, and fraction II is cerise oily mater, and fraction III and fraction IV are dark red oil
Matter.The thin-layer chromatography condition of Fig. 1 is：Using silica gel thin-layer chromatography plate, rowed dry a point with pencil at away from chromatoplate bottom 1cm
Collected each equidistant point sample of reception pipe eluent is carried out layer in being placed on line-transect in the chromatography cylinder containing solvent by line-transect
Analysis, solvent is eluent A or eluent B, Fig. 1 (a), 1 (b) and 1 (c) used solvent is eluent A, and 1 (d) is used
Solvent is eluent B, can reach preferably detection result under these conditions.Through the antitumor activity inspection to tumour cell
It surveys, the young grain acute leukemia cells system HL60 early to people of fraction II, human tissue cell's property lymphoma cell line U937 have growth
Inhibiting effect, and to Human normal hepatocyte HL7702 without growth inhibition effect, other three kinds of fractions are to above-mentioned cell without apparent
Effect.
(3) purifying of fraction II
By the fraction II obtained in step (2) by volume 1:3 use dilution in acetonitrile, mixing, then are splined on and prepare efficient liquid
In phase chromatographic column, wherein chromatographic column is that YMC prepares column (YMC-Pack ODS-A, 20 × 250mm, 10 μm of grain size), each sample introduction
0.6mL, using acetonitrile-water system as eluent, with the speed isorheic elution of 15mL/min, elution program is：It 0-10 minutes, uses
65% acetonitrile solution elutes；It 10-20 minutes, is eluted with 70% acetonitrile solution；It 20-60 minutes, is eluted with 75% acetonitrile.It is purple
Light absorption value at outer detection 210nm, spectrogram are as shown in Figure 2.Fractional Collections are carried out according to appearance time, 5 kinds of substances are obtained,
It is spare with Rotary Evaporators removal solvent according to appearance time successively marked as II -1 to II -5.It is diluted to suitable concentration with DMSO
Growth inhibition effect of the various substances to MCF-7 is detected using mtt assay afterwards, testing result shows that II -2 deposits with II -4 couple of MCF-7
In different degrees of growth inhibition effect (as shown in Figure 3,4), and II -1, II -3 and II -5 couple of MCF-7 is without growth inhibition effect.
Fig. 3 is the results show that the growth inhibition effect of -2 couples of MCF-7 of compositionⅱ is improved with the increase of concentration, in 12.5 μ g/mL of concentration
When inhibiting rate reach 59%.Fig. 4 results show that the growth inhibition effect of -4 couples of MCF-7 of compositionⅱ is carried with the increase of concentration
Height, in 12.5 μ g/mL of concentration, inhibiting rate reaches 71%.
(4) Structural Identification of compositionⅱ -2 and II -4
By the compositionⅱ -2 obtained in step (3) and II -4 through HRESIMS,1H-NMR and13C-NMR is detected, as a result
Such as Fig. 5-10.Fig. 5-6 illustrates that the ms fragment relative molecular mass of both substances is respectively 320.2570 and 346.2707, pushes away
Surveying may be to add NH4+Peak, be computed the practical relative molecular mass of both substances be 302.2246 and 328.2402, respectively with
The relative molecular mass data of EPA and DHA are coincide；Two kinds of substances1H-NMR spectrum (Fig. 7-8) and EPA and DHA in document1H-NMR spectrum relatively coincide, and concrete outcome is shown in Table 1；Two kinds of substances13C-NMR spectrograms (Fig. 9-10) also with data are reported in document
Unanimously, therefore it can determine that compositionⅱ -2 and II -4 is EPA and DHA in omega-3 polyunsaturated fatty acids respectively.
1. two kinds of active principles of table II -2 and II -41The measurement result of H-NMR spectrum
Laser raman scattering spectrum (Figure 11,12) display of EPA and DHA in antarctic krill oil and commercially available fish oil, two kinds are not
With source EPA and DHA Raman spectrum in displacement 1658cm-1、1671cm-1、1265cm-1And 1303cm-1Exist everywhere apparent
Difference, at preceding two displacement respectively represent Z-type carbon carbon unsaturated double-bond and E type unsaturated double-bonds, then at two displacement ratio [ν
(1303cm-1)/ν(1265cm-1)] bigger, indicate that Z-type carbon-carbon double bond number is more in sample.It is extracted in antarctic krill oil
Apparent E types unsaturated double-bond, therefore EPA and DHA in antarctic krill oil are can't see in the raman scattering spectrum of EPA and DHA
Z-type unsaturated carbon carbon number of double bonds be less than raman scattering spectrum detection limit, it can thus be assumed that the EPA and DHA be alltrans
EPA and DHA, and the unsaturated carbon-carbon double bonds of two kinds of E and Z are then existed simultaneously in commercially available fish oil EPA and DHA, Z-type will be far more than E types.
The antitumor activity of embodiment 2 antarctic krill oil EPA and DHA detect
Isolated E type EPA and DHA has inhibiting effect to the growth of kinds of tumor cells in antarctic krill oil,
IC50Value is shown in Table 2, these numerical value be below control group standard items EPA and DHA, sturgeon liver and commercially available fish oil source EPA and DHA
Correspondence IC50Value, and compared with currently marketed many antitumor drugs, krill EPA and DHA is to Human normal hepatocyte
HL7702 without overt toxicity, therefore application it is upper its with larger advantage, it may have wide application prospect.
Claims (7)
1. a kind of separation method of natural alltrans EPA or alltrans DHA with anti-tumor activity, includes the following steps：
(1) antarctic krill oil is added into silicagel column and carries out column chromatography for separation, collect eluent, removed solvent, obtain fraction I
With fraction II, fraction I is yellow oil, and fraction II is cerise grease；
(2) dilution in acetonitrile of fraction II, is detached with high performance preparative liquid chromatography method, with the mixed solvent system of acetonitrile and water
It is eluted for mobile phase, collects elution gained different component, remove solvent, obtain compositionⅱ -1 successively to II -5, wherein group
It is alltrans EPA to divide II -2, and compositionⅱ -4 is alltrans DHA.
2. separation method according to claim 1, which is characterized in that described in the column chromatography for separation described in step (1)
Eluent be volume ratio be 7:3-9:1 n-hexane and ether mixed liquor, addition percent by volume is 1-5%'s in mixed liquor
Acetic acid.
3. separation method according to claim 1, which is characterized in that the grain size of the silica gel described in step (1) be 300~
The mass ratio of 400 mesh, silica gel and antarctic krill oil is 30~100:1, chromatographic column ratio of height to diameter is 10~16:1.
4. separation method according to claim 1, which is characterized in that the high performance preparative liquid chromatography described in step (2)
Column packing is reverse phase C18, 10 μm of grain size, the elution program of mobile phase is as follows successively：Between eluting 0-10min, with 62-68% second
Nitrile elutes；Between eluting 10-20min, eluted with 67-73% acetonitriles；Between eluting 20-60min, eluted with 72-78% acetonitriles.
5. natural alltrans EPA or alltrans DHA that separation method as described in claim 1 obtains are preparing antitumor drug
In application.
6. application according to claim 5, which is characterized in that the tumour is human breast carcinoma, hepatocellular carcinoma, early young grain is white
Blood disease, chronic myelocytic leukemia, histiocytic lymphoma or prostate cancer.
7. a kind of pharmaceutical composition comprising the natural alltrans DHA and pharmacy that separation method as described in claim 1 obtains
Upper acceptable auxiliary material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610421280.2A CN106117044B (en) | 2016-06-15 | 2016-06-15 | The method that alltrans aliphatic acid with anti-tumor activity is detached from antarctic krill oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610421280.2A CN106117044B (en) | 2016-06-15 | 2016-06-15 | The method that alltrans aliphatic acid with anti-tumor activity is detached from antarctic krill oil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106117044A CN106117044A (en) | 2016-11-16 |
| CN106117044B true CN106117044B (en) | 2018-11-09 |
Family
ID=57270081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610421280.2A Active CN106117044B (en) | 2016-06-15 | 2016-06-15 | The method that alltrans aliphatic acid with anti-tumor activity is detached from antarctic krill oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106117044B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111362790B (en) * | 2020-04-17 | 2022-12-13 | 辽宁科技大学 | Chromatographic method for separating EPA and DHA |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105055402A (en) * | 2015-07-20 | 2015-11-18 | 西安膳方医药科技有限公司 | Composition of omega-3 series of poyenoic fatty acids and natural products |
-
2016
- 2016-06-15 CN CN201610421280.2A patent/CN106117044B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105055402A (en) * | 2015-07-20 | 2015-11-18 | 西安膳方医药科技有限公司 | Composition of omega-3 series of poyenoic fatty acids and natural products |
Non-Patent Citations (3)
| Title |
|---|
| FA Composition of Plasma, Red Blood Cell, and Liver Phospholipids of Newborn Piglets Fed trans Isomers of α-Linolenic Acid;Niyazi Acar et al.;《Lipids》;20021231;第37卷(第9期);849-852 * |
| Safety assessment of SuperbaTM krill powder:Subchronic toxicity study in rats;Kjetil Berge et al.;《Toxicology Reports》;20141128(第2期);144-151 * |
| 二十二碳六烯酸和二十碳五烯酸研究进展(1);许友卿等;《生物学通报》;20071231;第42卷(第11期);13-15 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106117044A (en) | 2016-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cha et al. | Antiproliferative effects of carotenoids extracted from Chlorella ellipsoidea and Chlorella vulgaris on human colon cancer cells | |
| Fan et al. | The anti-inflammatory activities of an extract and compounds isolated from Platycladus orientalis (Linnaeus) Franco in vitro and ex vivo | |
| de Los Reyes et al. | Molecular characterization and anti-inflammatory activity of galactosylglycerides and galactosylceramides from the microalga Isochrysis galbana | |
| La Clair et al. | Ganodone, a bioactive benzofuran from the fruiting bodies of Ganoderma tsugae | |
| CN104825500B (en) | Ganoderma leucocontextum extract, extraction method and application thereof | |
| Yang et al. | Analysis of Hydroxy Fatty Acids from the Pollen of Brassica campestris L. var. oleifera DC. by UPLC-MS/MS | |
| Heng et al. | Analysis of the bioactive components of Sapindus saponins | |
| Zhang et al. | Current advances on the structure, bioactivity, synthesis, and metabolic regulation of novel ubiquinone derivatives in the edible and medicinal mushroom Antrodia cinnamomea | |
| Yoon et al. | A direct protein kinase B-targeted anti-inflammatory activity of cordycepin from artificially cultured fruit body of Cordyceps militaris | |
| Orlando et al. | Vitamin K1 exerts antiproliferative effects and induces apoptosis in three differently graded human colon cancer cell lines | |
| Nunes et al. | Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira | |
| SP et al. | Isolation of methyl gamma linolenate from Spirulina platensis using flash chromatography and its apoptosis inducing effect | |
| Silva et al. | Microalgae as potential sources of bioactive compounds for functional foods and pharmaceuticals | |
| Qin et al. | Anti-inflammatory activity of isobutylamides from Zanthoxylum nitidum var. tomentosum | |
| CN105017345B (en) | The method of four kinds of compounds of extraction and the application simultaneously from selfheal | |
| Chang et al. | Simultaneous determination of ten active components in traditional Chinese medicinal products containing both Gegen (Pueraria lobata) and Danshen (Salvia miltiorrhiza) by high‐performance liquid chromatography | |
| Soltani et al. | Cordycepin: A biotherapeutic molecule from medicinal mushroom | |
| CN103087031A (en) | Application of bistetrahydrobenzopyrone dimer compounds in anti-cancer drugs | |
| CN106117044B (en) | The method that alltrans aliphatic acid with anti-tumor activity is detached from antarctic krill oil | |
| Wei et al. | Triterpenoids as bivalent and dual inhibitors of acetylcholinesterase/butyrylcholinesterase from the fruiting bodies of Inonotus obliquus | |
| Richbart et al. | Nonpungent N-AVAM Capsaicin Analogues and Cancer Therapy | |
| CN101343247B (en) | Cyclohexenone extract of antrodia camphorata | |
| CN102247357B (en) | Application of uvarilactam and aristololactam BI in preparation of anticancer medicaments | |
| Wang et al. | Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS | |
| WO2010025589A1 (en) | Long acting curcumine derivates and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |