CN106093380A - antibiotic test paper - Google Patents
antibiotic test paper Download PDFInfo
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- CN106093380A CN106093380A CN201610528012.0A CN201610528012A CN106093380A CN 106093380 A CN106093380 A CN 106093380A CN 201610528012 A CN201610528012 A CN 201610528012A CN 106093380 A CN106093380 A CN 106093380A
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- antibiotic
- detection line
- gold label
- test paper
- antibiotics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biomedical Technology (AREA)
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- Hematology (AREA)
- Urology & Nephrology (AREA)
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- Food Science & Technology (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of antibiotic Test paper, including sample absorption pad, gold mark thing pad, reaction film and absorption paper, gold-marking binding pad is coated with beta-lactam specificity antibody colloidal gold label, tetracycline specificity antibody colloidal gold label, quinolinones specificity antibody colloidal gold label, macrocyclic lactone specificity antibody colloidal gold label, sulfanilamide specificity antibody colloidal gold label, cephalo specificity antibody colloidal gold label.Reaction film is coated with macrolide antibiotic detection line, beta-lactam class antibiotic detection line, tetracycline antibiotics detection line, quinolone antibiotic detection line, sulfa antibiotics detection line, cephalosporins detection line, sheep anti mouse nature controlling line.In child's urine of the present invention, the joint-detection reagent paper of Multiple Classes of Antibiotics is easy to use, quickly, it is not necessary to professional operation, is suitable for family and uses.
Description
Technical field
The present invention relates to a kind of child's diaper with urine detection function, and a kind of urine detection method.
Background technology
Fudan University one exceedes thousand child's urine Inspection Research show for Jiangsu, Zhejiang, Shanghai, the Shanghai of 79.6%
Detecting one or more in 21 kinds of antibiotic in school age population urine, the frequency that more than a kind antibiotic is found in urine is
58.3%, the frequency that at least two and above antibiotic or antibiotic classification find in urine simultaneously is respectively 26.7% He
23.5%, and in a urine sample, multipotency detects 6 kinds of antibiotic of 4 class simultaneously；In urine, antibiotic total concentration sum is often
Urine sample between milliliter 0.1-20 nanogram accounts for 47.8%, and part urine sample antibiotic concentration exceedes every milliliter of 1000 nanograms.This extensively
General exposed state may increase the weight of bacterial resistance, thus threatens clinical treatment, it is also possible to growth promoter and the population health to child
Cause potentially hazardous.World Health Organization (WHO) (WHO) once issued report expression, and it is each that antibiotic resistant bacteria is just spreading to the whole world
Ground.(April 17 " Reference News ").Antibiotics resistance, as bad haze weather, can gradually develop into a kind of environment danger
Machine, threatens everyone.Drug use is improper to be caused and aggravates the microorganisms such as antibacterial and change, and finally results in and is used in treatment
The medicine infected becomes invalid, here it is the source of antibiotic resistance.In animal husbandry, antibiotic is widely used and can cause antibiosis
The development of element drug resistance.After antibiotic resistance is propagated in domestic animal, can be existed by food origin disease and other routes of infection again
Crowd is spread.The urine of child detecting, animal husbandry common antibiotics is exactly a typical example.And with human body
Improper to drug use, will also result in antibiotic resistance.The antibiotic detecting child's urine currently without mode easily contains
Amount.
At present, to the detection of antibiotic mainly at field of food and environmental area, for the method master of antibiotic detection
High-efficient liquid phase chromatogram technology to be had, microbial method, immunoassay, these methods need expensive detecting instrument or the inspection of specialty
Survey personnel, are not suitable for family and use, also not for the method for quick of antibiotic in child's urine.
Summary of the invention
The technical problem to be solved is to provide a kind of antibiotic Test paper, it is possible to multiple in child's urine
The joint-detection of antibiotic, easy to use, quick, it is not necessary to professional operation, it is suitable for family and uses.
In order to solve above technical problem, the invention provides a kind of antibiotic Test paper, including sample absorption pad, sample
Product pad, reaction film and sample adsorptive pads.
Preferably, the antibiotic Test paper of the present invention, described sample adsorptive pads is filter paper block.
Preferably, the antibiotic Test paper of the present invention, described sample pad is coated with beta-lactam specificity and resists
Body-colloid gold label thing, tetracycline specific antibody-colloid gold label thing, quinolinones specific antibody-colloid gold label thing,
Macrocyclic lactone specific antibody-colloid gold label thing, sulfanilamide specific antibody-colloid gold label thing and cephalo specific antibody-
Colloid gold label thing.
Preferably, the antibiotic Test paper of the present invention, described reaction film is nitrocellulose filter, wraps according to this above it
There are macrolide antibiotic detection line, beta-lactam antibiotic detection line, tetracycline antibiotics detection line, quinolinones
Class antibiotic detection line, sulfa antibiotics detection line, cephalosporins detection line and sheep anti mouse nature controlling line.
Further, the antibiotic Test paper of the present invention, described macrolide antibiotic detection line is with described
Beta-Lactam antibiotic detection line is side by side, described tetracycline antibiotics detects line and described quinolone antibiotic detects
Line is side by side, described sulfa antibiotics detects line and described cephalosporins detects line side by side.
Further, the antibiotic Test paper of the present invention, in described macrolide antibiotic detection line is macro ring
Lipid antibiotic-carrier protein, described beta-lactam antibiotic detection line is beta-lactam antibiotic-carrier protein,
Described tetracycline antibiotics detection line is tetracycline antibiotics-carrier protein, described quinoline promise alkane ketone antibiotic inspection
Survey line is quinoline promise alkane ketone antibiotic-carrier protein, and described sulfa antibiotics detection line is sulfa antibiotics-carrier egg
In vain, described cephalosporins detection line is that cephalosporins detects line.
Further, the antibiotic Test paper of the present invention, described carrier protein is bovine serum albumin.
The antibiotic Test paper of the present invention, it is possible to the joint-detection of Multiple Classes of Antibiotics in child's urine, easy to use,
Quickly, it is not necessary to professional operation, it is suitable for family to use.
Accompanying drawing explanation
With detailed description of the invention, the present invention is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 is the structural representation of the present invention.
Detailed description of the invention
The antibiotic Test paper of the present invention includes sample absorption pad, sample pad, reaction film and sample adsorptive pads.
Described sample adsorptive pads is filter paper block.
Described sample pad is coated with beta-lactam specific antibody-colloid gold label thing, tetracycline specificity resists
Body-colloid gold label thing, quinolinones specific antibody-colloid gold label thing, macrocyclic lactone specific antibody-colloid gold label
Thing, sulfanilamide specific antibody-colloid gold label thing and cephalo specific antibody-colloid gold label thing.
Described reaction film is nitrocellulose filter, be coated with according to this above it macrolide antibiotic detection line, β-
Lactam antibiotics detection line, tetracycline antibiotics detection line, quinolone antibiotic detection line, sulfa antibiotics inspection
Survey line, cephalosporins detection line and sheep anti mouse nature controlling line.
Described macrolide antibiotic detection line and described beta-Lactam antibiotic detection line side by side, described four
Ring element class antibiotic detection line and described quinolone antibiotic detection line side by side, described sulfa antibiotics detection line with
Described cephalosporins detection line is side by side.
Described macrolide antibiotic detection line is macrolide antibiotic-carrier protein, described β-interior acyl
Amine antibiotic detection line is beta-lactam antibiotic-carrier protein, and described tetracycline antibiotics detection line is Fourth Ring
Element class antibiotic-carrier protein, described quinoline promise alkane ketone antibiotic detection line is quinoline promise alkane ketone antibiotic-carrier protein,
Described sulfa antibiotics detection line is sulfa antibiotics-carrier protein, and described cephalosporins detection line is head
Spore class antibiotic detection line.Described carrier protein is bovine serum albumin.
A kind of preparation method of gold colloidal:
Mass fraction 5% gold chloride is diluted to mass fraction to 0.01% by double distilled deionized water, puts magnetic force heating rod
On agitator, stirring is boiled, and after boiling, every 100ml gold chloride adds 5ml mass fraction 3% trisodium citrate, the color meeting of solution
By pale yellow change blue, then deepen orchid, eventually become redness, when the color of solution become completely transparent red time, continue back
Stream, stops heating, is supplemented to original volume by double distilled deionized water and keeps in Dark Place in 4 DEG C after being cooled to room temperature after 10min.
A kind of beta-lactam antibiotic specific antibody-colloid gold label thing preparation method:
(1) determine gold colloidal and antibody usage ratio to be marked with ocular estimate, regulate colloid with the potassium carbonate of 0.1mol/L
Antibody pH, to 7.2, is 7.6 by gold solution, and the phosphate buffer stepwise dilution of 0.1mol/L is 5～40 μ g/ml, respectively takes
0.1ml is sequentially added in a series of test tube equipped with 1ml gold colloidal, is separately added into 0.1ml matter after 5min in above-mentioned test tube
Amount mark 1% sodium chloride, mixing, stand observed result after 2h.From the beginning of adding the test tube that antibody concentration is 10 μ g/ml, colloid
The color of gold solution is basically identical, and therefore stablizing the suitableeest amount of antibody of 1ml gold colloidal is 10 μ g/ml.
(2) under magnetic stirring, 10ml gold colloidal adds beta-lactam antibiotic specificity grand antibody 10ml, continues
Continuous stirring and evenly mixing 10min, adds 0.2ml10%BSA, stands 30min.4 DEG C of centrifugal 30min, abandon supernatant, and precipitate is with containing matter
The amount bovine serum albumin of mark 0.1%, the surfactant of 0.5%, pH is the 0.2mol/L phosphate buffer washing of 7.2
Twice, the golden labeling antibody solution of i.e. available purification, put 4 DEG C standby.Prepare other gold labeling antibody solution in the same way.
The sensitivity test of antibiotic test strip
Macrolide antibiotic, β-interior phthalein amine antibiotic, tetracycline antibiotics, quinoline promise alkane ketone antibiotic, head
Spore class antibiotic and sulfa antibiotics are diluted to variable concentrations, so with the phosphate buffer of the 0.2mol/L that pH is 7 respectively
Detect by the test strips of the present invention afterwards, the antibiotic of variable concentrations is added drop-wise to the sample pad of test strips.Result shows, this
The detection sensitivity of test strips is respectively as follows: erythromycin 10 μ g/L, spiramycin 8 μ g/L, benzylpenicillin 4 μ g/L, amoxicillin 4 μ
G/L, chlortetracycline 15 μ g/L, oxytetracycline 30 μ g/L, norfloxacin 60 μ g/L, ofloxacin 100 μ g/L, sulfadoxine 100 μ g/L,
Sulfanilamide crash smack one's lips 100 μ g/L, head embrace promise 20 μ g/L, head embraces Luoning 20 μ g/L.
The antibiotic Test paper of the present invention, it is possible to the joint-detection of Multiple Classes of Antibiotics in child's urine, easy to use,
Quickly, it is not necessary to professional operation, it is suitable for family to use.
Claims (7)
1. an antibiotic Test paper, it is characterised in that include that sample absorption pad, sample pad, reaction film and sample are inhaled
Water cushion.
2. antibiotic Test paper as claimed in claim 1, it is characterised in that described sample adsorptive pads is filter paper block.
3. antibiotic Test paper as claimed in claim 1, it is characterised in that described sample pad is coated with β-interior acyl
Amine specific antibody-colloid gold label thing, tetracycline specific antibody-colloid gold label thing, quinolinones specific antibody-colloid
Gold label, macrocyclic lactone specific antibody-colloid gold label thing, sulfanilamide specific antibody-colloid gold label thing and cephalo are special
Heterogenetic antibody-colloid gold label thing.
4. antibiotic Test paper as claimed in claim 1, it is characterised in that described reaction film is nitrocellulose filter,
Macrolide antibiotic detection line, beta-lactam antibiotic detection line, tetracycline antibiotics it is coated with according to this above it
Detection line, quinolone antibiotic detection line, sulfa antibiotics detection line, cephalosporins detection line and sheep anti mouse Quality Control
Line.
5. antibiotic Test paper as claimed in claim 4, it is characterised in that described macrolide antibiotic detection line
With described beta-Lactam antibiotic detection line side by side, described tetracycline antibiotics detection line resists with described quinolones
Life element detection line is side by side, described sulfa antibiotics detects line and described cephalosporins detects line side by side.
6. antibiotic Test paper as claimed in claim 4, it is characterised in that described macrolide antibiotic detection line
For macrolide antibiotic-carrier protein, described beta-lactam antibiotic detection line is beta-lactam antibiotic-load
Body protein, described tetracycline antibiotics detection line is tetracycline antibiotics-carrier protein, and described quinoline promise alkanone class resists
Raw element detection line is quinoline promise alkane ketone antibiotic-carrier protein, described sulfa antibiotics detection line be sulfa antibiotics-
Carrier protein, described cephalosporins detection line is that cephalosporins detects line.
7. antibiotic Test paper as claimed in claim 6, it is characterised in that described carrier protein is bovine serum albumin
In vain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610528012.0A CN106093380A (en) | 2016-07-06 | 2016-07-06 | antibiotic test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610528012.0A CN106093380A (en) | 2016-07-06 | 2016-07-06 | antibiotic test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106093380A true CN106093380A (en) | 2016-11-09 |
Family
ID=57213469
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610528012.0A Pending CN106093380A (en) | 2016-07-06 | 2016-07-06 | antibiotic test paper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106093380A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771158A (en) * | 2016-11-30 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of Ofloxacin Test paper and its detection method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005075998A1 (en) * | 2004-01-29 | 2005-08-18 | Dsm Ip Assets B.V. | Improved lateral flow binding assay |
| CN201518027U (en) * | 2009-07-24 | 2010-06-30 | 杭州南开日新生物技术有限公司 | Tetracycline immune colloidal gold quick test reagent board |
| CN203630142U (en) * | 2013-11-01 | 2014-06-04 | 洛阳莱普生信息科技有限公司 | Antibiotic quadruple rapid detection card |
| CN105424924A (en) * | 2015-11-02 | 2016-03-23 | 广州璞雅医药生物科技有限公司 | Antibiotic test paper strip and preparation method and application thereof |
-
2016
- 2016-07-06 CN CN201610528012.0A patent/CN106093380A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005075998A1 (en) * | 2004-01-29 | 2005-08-18 | Dsm Ip Assets B.V. | Improved lateral flow binding assay |
| CN201518027U (en) * | 2009-07-24 | 2010-06-30 | 杭州南开日新生物技术有限公司 | Tetracycline immune colloidal gold quick test reagent board |
| CN203630142U (en) * | 2013-11-01 | 2014-06-04 | 洛阳莱普生信息科技有限公司 | Antibiotic quadruple rapid detection card |
| CN105424924A (en) * | 2015-11-02 | 2016-03-23 | 广州璞雅医药生物科技有限公司 | Antibiotic test paper strip and preparation method and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771158A (en) * | 2016-11-30 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of Ofloxacin Test paper and its detection method |
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Application publication date: 20161109 |
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