CN106092979A - Detector array is utilized to realize the dental plaque quantitative testing device of fluorescent energy ratio - Google Patents
Detector array is utilized to realize the dental plaque quantitative testing device of fluorescent energy ratio Download PDFInfo
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- CN106092979A CN106092979A CN201610374067.0A CN201610374067A CN106092979A CN 106092979 A CN106092979 A CN 106092979A CN 201610374067 A CN201610374067 A CN 201610374067A CN 106092979 A CN106092979 A CN 106092979A
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- detector array
- dental plaque
- testing device
- fluorescent energy
- signal processing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
- G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
Abstract
The invention discloses the dental plaque quantitative testing device utilizing detector array to realize fluorescent energy ratio, including light source (1), focussed collimated parts (2), dichroic mirror (3), phosphor collection parts (5), dispersion element (6), detector array (7), timing sequencer (8), a/d converter (9), transmission controls and signal processing module (10), display (11), after light line focus collimating components (2) that light source (1) sends, and through phosphor collection parts (5) after dichroic mirror (3) reflection turns to, it is irradiated on tooth samples (4)；The fluorescence that tooth samples (4) is excited is after phosphor collection parts (5), through dichroic mirror (3), incide on dispersion element (6), detector array (7) is set after dispersion element (6), detector array (7) is connected with timing sequencer (8), timing sequencer (8) is connected with a/d converter (9), a/d converter (9) controls with transmission and signal processing module (10) is connected, and transmission controls and signal processing module (10) is connected with display (11).
Description
Technical field
The invention belongs to oral health monitoring technical field, relate to dental plaque detection device, specifically one and be used for detecting
Oral plaque content and the device of distribution.
Background technology
Dental plaque is a kind of to be attached on dental surface, bacillary thin film based on stickiness substrate.Thin in dental plaque
Toxin produced by bacterium and other harmful substances directly stimulate gingiva (being commonly called as dental bed), make root of the tooth inflammation.In dental plaque new old generation, is new,
Decompose sugar and produce acidifying substance, destroy the dynamic equilibrium in dental hard tissue's demineralization ore deposit again, cause demineralization cariogenic.Dental plaque is dental caries
Sick and that periodontal disease is common main pathogenic.Dental plaque is detected oral cavity disease prevention significant.Due to
People's naked eyes cannot directly observe dental plaque, and current clinic typically uses plaque disclosing agent, developer type to have liquor and tablet two
Kind.When using liquor, can see being moistened with the cotton balls liquid spreading of display liquid in the cheek lingual surface of dental pattern and adjacent gap location after gargling
Examine；Bacterial plaque also can being shown, drop, in the tip of the tongue, licks each facing with the tip of the tongue, observes after gargling.When using tablet, tablet is chewed,
With the tip of the tongue, fragment is licked each facing, observe after gargling.The dyeing of attachment area without bacterial plaque is washed off, and has the region that bacterial plaque is adhered to
Then present certain dyeing.The method uses the mode that outside agent is smeared, and developer remains in oral cavity not easy cleaning, according to naked eyes
Observe and judge that the mode of plaque distribution has subjectivity, it is impossible to realize quantitative NDT.
Dental plaque will produce autofluorescence under ultraviolet excitation, be different from healthy tissue of tooth and have different fluorescence spies
Levy spectral band, utilize this wave band fluorescent energy can dental plaque content and distribution be detected.
The technology utilizing fluorescent technique detection dental plaque is disclosed, such as: Chinese patent literature ZL201110419562.6
Disclose one " utilize face battle array imaging dental plaque detection device ", use 405nm light source activation dental surface, by
The method placing optical filter before planar array detector extracts the fluorescence of 600-800nm, shows that dental plaque is distributed in the form of images.Again
As, Chinese patent literature CN1309545 discloses one " for the toothbrush with fluorescent apparatus of dental plaque detection ", at tooth
Surface scan, excitation source wavelength is at 470 ± 40nm, and detection wavelength is at about 540-550nm and 610-620nm.Autonomous test in the future
The fluorescence intensity of tooth surface compares with the fluorescence intensity from clean tooth surface, judges the content of dental plaque in this approach,
Realize the detection of dental plaque.
Summary of the invention
The invention provides a kind of dental plaque content quantitative utilizing Autofluorescence energy ratio under ultraviolet excitation
The cannot-harm-detection device of detection, its entitled dental plaque detection by quantitative dress utilizing detector array to realize fluorescent energy ratio
Putting, it can carry out direct detection to the content of dental plaque, and provides testing result with the form of numerical value, has noncontact, determines
The features such as quantization.
The present invention is by the following technical solutions:
Utilize detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio, including light source (1), focus on standard
Straight parts (2), dichroic mirror (3), phosphor collection parts (5), dispersion element (6), detector array (7), timing sequencer
(8), a/d converter (9), transmission control and signal processing module (10), display (11), the light line focus standard that light source (1) sends
After straight parts (2), and through phosphor collection parts (5) after dichroic mirror (3) reflection turns to, it is irradiated to tooth samples (4)
On.Focussed collimated parts (2), dichroic mirror (3), phosphor collection parts (5) are installed on the transmission direction of light source (1) successively.Tooth
The fluorescence that tooth sample (4) is inspired, after phosphor collection parts (5), through dichroic mirror (3), incides dispersion element
(6) on, placing detector array (7) below at dispersion element (6), detector array (7) is connected with timing sequencer (8), AD
Transducer (9) is connected with timing sequencer (8), and under timing sequencer (8) controls, the data of detector array (7) enter AD
Transducer (9), a/d converter (9) controls with transmission and signal processing module (10) is connected, and the data of a/d converter (9) enter and pass
Defeated control and signal processing module (10), after treatment, output display is in display (11).
Preferably, light source (1) is the light source such as semiconductor laser or LED.
Preferably, narrow in the range of the wave-length coverage of light source (1) sent light is 400-410nm, or 400-410nm
Band.
Preferably, by dichroic mirror (3), exciting light is reflected, and to Fluoroscopic.
Preferably, light beam is carried out shaping by focussed collimated parts (2), is adjusted to elongate light beam, the light being irradiated on tooth
Speckle size is about 0.2mm.
Preferably, dichroic mirror (3) installation direction and phosphor collection parts (5) optical axis direction angle 45 degree.
Preferably, dichroic mirror (3) is less than the luminous reflectance of 420nm to wavelength, for the light transmission more than 420nm.
Preferably, utilize dispersion element (6) fluorescence spectrum spatially to be separated, dispersion element (6) be grating or
Prism, or other have the components and parts of dispersion function.
Preferably, detector array (7) is linear array detector or planar array detector.
Preferably, transmission controls and the data of detector array (7) are processed by signal processing module (10), obtains
Fluorescent energy value a of 420nm-600nm scope and spectral energy value b of 600nm-800nm scope, and calculate a/b or b/a
Ratio as two energy.
The invention also discloses another kind of technical scheme:
Utilize detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio, including light source (1), focus on standard
Straight parts (2), phosphor collection parts (5), dispersion element (6), detector array (7), timing sequencer (8), a/d converter
(9), control and signal processing module (10), display (11) are transmitted, after light line focus collimating components (2) that light source (1) sends,
It is irradiated on tooth samples (4).Focussed collimated parts (2) are installed on the transmission direction of light source (1) successively.Tooth samples (4) institute
The fluorescence inspired, after phosphor collection parts (5), incides on dispersion element (6), places battle array below at dispersion element (6)
Row detector (7), detector array (7) is connected with timing sequencer (8), and a/d converter (9) is connected with timing sequencer (8),
Under timing sequencer (8) controls, the data of detector array (7) enter a/d converter (9), a/d converter (9) and transmission control
System and signal processing module (10) are connected, and the data of a/d converter (9) enter transmission and control and signal processing module (10), pass through
After process, output display is in display (11).
Preferably, light source (1) is the light source such as semiconductor laser or LED.
Preferably, narrow in the range of the wave-length coverage of light source (1) sent light is 400-410nm, or 400-410nm
Band.
Preferably, light beam is carried out shaping by focussed collimated parts (2), is adjusted to elongate light beam, in oblique illumination to tooth
Spot size be about 0.2mm.
Preferably, dispersion element (6) can be grating or prism.
Preferably, detector array (7) is linear array detector or planar array detector.
Preferably, transmission controls and the data of detector array (7) are processed by signal processing module (10), obtains
Fluorescent energy value a of 420nm-600nm and spectral energy value b of 600nm-800nm, and calculate a/b or b/a as two
The ratio of energy.
After light line focus collimating components (2) that light source (1) sends, in oblique illumination to tooth samples (4), with phosphor collection
The optical axis of parts (5) has certain angle, and angle is between 0-90 degree.
The present invention utilizes detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio, and dental plaque is in ultraviolet
Light excites lower generation to be different from the fluorescence spectrum of dental health tissue, and action of ultraviolet light is at fluorescent light source produced by dental surface
In dental health tissue and dental plaque, the fluorescence spectrum of dental health tissue is mainly distributed between 420-600nm, dental plaque
Fluorescence spectrum is mainly distributed between 600-800, and the fluorescence spectrum excited is separated in space by dispersion means, by dispersion
Placing detector array after device and receive fluorescence spectrum, different spectrum are positioned at the diverse location of detector array, by signal
The energy value of different spectral bands is calculated by reason module, shows over the display the most in digital form.Glimmering owing to using
The method detection dental plaque content of light spectral energy ratio, compares the method only detecting dental plaque fluorescent energy, and it is objective to have more
Property, accuracy.
The dental plaque detection by quantitative of fluorescence spectrum energy ratio of the present invention, can be with objective and accurate quantitative response sensing point tooth bacterium
Speckle content, carries out detection by scanning boil on the nape opposite the mouth intracavity every bit and can obtain the distribution situation of bacterial plaque, and this invention is for instructing oral cavity
Health care and prevention oral disease are significant.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment one.
Fig. 2 is the structural representation of embodiment two.
Fig. 3 is transmission control and signal processing module inner function module figure.
Detailed description of the invention
The present invention utilizes detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio, according to exciting light and
The difference of phosphor collection structure, has various embodiments, below in conjunction with the accompanying drawings, to two of which is typical and preferred embodiment is given
To describe in detail.
Embodiment one:
Fig. 1 shows exciting light and the embodiment of phosphor collection light path coaxial, and it includes light source (1), focussed collimated parts
(2), dichroic mirror (3), phosphor collection parts (5), spectral dispersion element (6), detector array (7), timing sequencer (8),
A/d converter (9), transmission control and signal processing module (10), display (11), and the emergent light direction of light source (1) is sequentially provided with
Focussed collimated parts (2), dichroic mirror (3), dichroic mirror (3) inclined shape.The side of dichroic mirror (3) arranges phosphor collection
Parts (5), opposite side arranges spectral dispersion element (6).The opposite side of phosphor collection parts (5) disposes tooth samples to be measured
(4).The opposite side of dispersion element (6) arranges detector array (7), and detector array (7) is linear array detector or the detection of face battle array
Device, detector array (7) is connected with timing sequencer (8), and a/d converter (9) is connected with timing sequencer (8), occurs in sequential
Under device (8) controls, the data of detector array (7) enter a/d converter (9), and a/d converter (9) and transmission control and at signal
Reason module (10) is connected, and the data of a/d converter (9) enter transmission and control and signal processing module (10), display (11) and biography
Defeated control and signal processing module (10) are connected.
Light source (1) can be in the range of 400-410nm a certain laser determining wavelength or centre wavelength for the most a certain
The LED of wavelength, the exciting light sent obtains the beam diameter directional light at about 0.2mm, light after focussed collimated parts (2)
Bundle incides on dichroic mirror (3) with 45 degree of angles, and the luminous reflectance less than 420nm, reflection light are compared incident illumination by dichroic mirror (3)
Having shifted towards 90 degree to incide on phosphor collection parts (5), incident illumination is irradiated to tooth along the optical axis direction of phosphor collection parts (5)
On sample (4) surface, the fluorescence packets inspired contains dental health tissue and the fluorescence of dental plaque, through phosphor collection parts (5)
Collecting and after shaping, incide on dichroic mirror (3), dichroic mirror belongs to long wave and leads to, by saturating for the wavelength light more than 420nm
Cross.The fluorescence passed through incides on dispersion element (6) with directional light, and dispersion element (6) has spectrum is carried out scattered function,
Can be grating or Amici prism.Fluorescence is after dispersion element (6), and spectrum is spatially distributed, by detector array (7)
To spectroscopically detectable, and the fluorescence realizing 420nm-800nm scope is collected.Under timing sequencer (8) controls, it is poised for battle
The analog data of row detector (7) is transmitted, and enters a/d converter (9), and a/d converter (9) is by the mould of detector array (7)
Intend data and be converted to digital quantity.Controller (101) in transmission control and signal processing module (10) controls synchronised clock (102)
The digital quantity of a/d converter (9) is transferred in memorizer (104) by input interface (103), can by controller (101)
Obtain the position of storage in memorizer (104) of the fluorescence data in the range of 420nm-600nm scope and 600nm-800nm
And the fluorescent energy size of each wavelength, by calculate arithmetical unit (105) fluorescent energy value a of 420nm-600nm scope with
Ratio a/b or b/a of spectral energy value b of 600nm-800nm scope, and this ratio is transmitted to output interface (106),
Display is on display (11) eventually.
Embodiment two:
Fig. 2 shows exciting light and the not coaxial embodiment of phosphor collection light path, and it includes light source (1), focussed collimated portion
Part (2), phosphor collection parts (5), spectral dispersion element (6), detector array (7), timing sequencer (8), a/d converter
(9), transmitting control and signal processing module (10), display (11), the emergent light direction of light source (1) is provided with focussed collimated parts
(2), focussed collimated parts (2) dispose tooth samples (4) to be measured along appearing light side, and tooth samples (4) and light are in necessarily
Angle.The radiation direction excited along tooth samples (4) is provided with phosphor collection parts (5).The light line focus collimation that light source (1) sends
After parts (2), in oblique illumination to tooth samples (4), the angle of the optical axis formation with phosphor collection parts (5) is at 0-90 degree.
The opposite side of phosphor collection parts (5) is provided with dispersion element (6), and the opposite side of dispersion element (6) arranges detector array (7),
Detector array (7) is connected with timing sequencer (8), and a/d converter (9) is connected, at timing sequencer with timing sequencer (8)
(8) under controlling, the data of detector array (7) enter a/d converter (9), and a/d converter (9) controls and signal processing with transmission
Module (10) is connected, and the data of a/d converter (9) enter transmission and control and signal processing module (10), display (11) and transmission
Control and signal processing module (10) is connected.
Light source (1) can be in the range of 400-410nm a certain laser determining wavelength or centre wavelength for the most a certain
The LED of wavelength, the exciting light sent obtains the beam diameter directional light at about 0.2mm, light after focussed collimated parts (2)
Bundle incides on tooth samples (4) surface at a certain angle, and the fluorescence packets inspired is glimmering containing dental health tissue and dental plaque
Light, after phosphor collection parts (5) collect also shaping, fluorescence incides on dispersion element (6) with directional light, dispersion element
(6) have spectrum is carried out scattered function, can be grating or Amici prism.Fluorescence is after dispersion element (6), and spectrum exists
Spatially being distributed, by detector array (7) to spectroscopically detectable, and the fluorescence realizing 420nm-800nm scope is received
Collection.Under timing sequencer (8) controls, the analog data of detector array (7) is transmitted, enters a/d converter (9), AD
The analog data of detector array (7) is converted to digital quantity by transducer (9).Transmission controls and in signal processing module (10)
Controller (101) controls synchronised clock (102) and is transferred into depositing by input interface (103) by the digital quantity of a/d converter (9)
In reservoir (104), the fluorescence spectrum in the range of 420nm-600nm scope and 600nm-800nm can be obtained by controller (101)
The position of data storage in memorizer (104) and the fluorescent energy size of each wavelength, calculated by arithmetical unit (105)
Fluorescent energy value a of 420nm-600nm scope and ratio a/b or b/a of spectral energy value b of 600nm-800nm scope, and
This ratio is transmitted to output interface (106), is eventually displayed on display (11).
The present invention utilizes detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio, and its light source sends purple
Outer light action is on tooth, and the fluorescence that dental plaque sends incides spectral dispersion device, by spectrum after collecting shaping
Place detector array after dispersion means, receive the fluorescent energy of 420nm-800nm scope, and through signal processing module meter
Calculate the energy ratio of two wave bands, show with numeric form, for the content of quantitative assessment dental plaque.
Those of ordinary skill in the art it should be appreciated that above example be intended merely to illustrate the present invention, and
It is not intended as limitation of the invention, as long as within the scope of the invention, change, the modification of above example all will be fallen at this
The protection domain of invention.
Claims (10)
1. utilize detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio, it is characterized in that including light source (1),
Focussed collimated parts (2), dichroic mirror (3), phosphor collection parts (5), dispersion element (6), detector array (7), sequential are sent out
Raw device (8), a/d converter (9), transmission control and signal processing module (10), display (11), and the light that light source (1) sends is through poly-
After focus collimation parts (2), and through phosphor collection parts (5) after dichroic mirror (3) reflection turns to, it is irradiated to tooth samples
(4) on；The fluorescence that tooth samples (4) is excited, after phosphor collection parts (5), through dichroic mirror (3), incides dispersion
On element (6), detector array (7), detector array (7) and timing sequencer (8) phase are set after dispersion element (6)
Even, timing sequencer (8) is connected with a/d converter (9), and a/d converter (9) controls and signal processing module (10) phase with transmission
Even, transmission controls and signal processing module (10) is connected with display (11).
Utilizing detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claim 1, it is special
Levy and be: light source (1) is semiconductor laser or LED.
Utilizing detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claim 1, it is special
Levy and be: dichroic mirror (3) is less than the luminous reflectance of 420nm to wavelength, for the light transmission more than 420nm.
Utilizing detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claim 1, it is special
Levy and be: dispersion element (6) is grating or prism.
Utilizing detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claim 1, it is special
Levy and be: detector array (7) is linear array detector or planar array detector.
6. utilize detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio, it is characterized in that including light source (1),
Focussed collimated parts (2), phosphor collection parts (5), dispersion element (6), detector array (7), timing sequencer (8), AD turn
Parallel operation (9), transmission control and signal processing module (10), display (11), the light line focus collimating components that light source (1) sends
(2) after, being irradiated on tooth samples (4), the fluorescence that tooth samples (4) is inspired is after phosphor collection parts (5), incident
On dispersion element (6), detector array (7), detector array (7) and timing sequencer are set after dispersion element (6)
(8) being connected, timing sequencer (8) is connected with a/d converter (9), and a/d converter (9) controls and signal processing module with transmission
(10) being connected, transmission controls and signal processing module (10) is connected with display (11).
Utilizing detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claim 6, it is special
Levy and be: the wave-length coverage of light source (1) sent light is the arrowband in the range of 400-410nm, or 400-410nm.
Detector array is utilized to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claims 6 or 7, its
Feature is: light beam is carried out shaping by focussed collimated parts (2), is adjusted to elongate light beam, and the hot spot in oblique illumination to tooth is big
Little for 0.1-0.3mm.
Utilizing detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claim 8, it is special
Levy and be: after light line focus collimating components (2) that light source (1) sends, in oblique illumination to tooth samples (4), with phosphor collection portion
The angle that the optical axis of part (5) is formed is at 0-90 degree.
Utilizing detector array to realize the dental plaque quantitative testing device of fluorescent energy ratio the most as claimed in claim 6, it is special
Levy and be: transmission controls and the data of detector array (7) are processed by signal processing module (10), obtains 420nm-600nm
Fluorescent energy value a and spectral energy value b of 600nm-800nm, and calculate a/b or the b/a ratio as two energy.
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| CN106770091A (en) * | 2016-11-28 | 2017-05-31 | 中国科学院合肥物质科学研究院 | A kind of isothermal portable fluorescence detector for detecting coronary heart disease |
| US20210178481A1 (en) * | 2018-04-23 | 2021-06-17 | Addup | Apparatus and method for manufacturing a three-dimensional object |
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