CN106085913B - A kind of complex microorganism preparations and preparation method thereof for sewage treatment - Google Patents
A kind of complex microorganism preparations and preparation method thereof for sewage treatment Download PDFInfo
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- CN106085913B CN106085913B CN201610520770.8A CN201610520770A CN106085913B CN 106085913 B CN106085913 B CN 106085913B CN 201610520770 A CN201610520770 A CN 201610520770A CN 106085913 B CN106085913 B CN 106085913B
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- 239000010865 sewage Substances 0.000 title claims abstract description 44
- 244000005700 microbiome Species 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 241000604933 Bdellovibrio Species 0.000 claims abstract description 13
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 241001136391 Thiobacillus denitrificans ATCC 25259 Species 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 11
- 239000002054 inoculum Substances 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 3
- 239000005995 Aluminium silicate Substances 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 235000012211 aluminium silicate Nutrition 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 239000000440 bentonite Substances 0.000 claims description 3
- 229910000278 bentonite Inorganic materials 0.000 claims description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 3
- 235000013379 molasses Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 244000025254 Cannabis sativa Species 0.000 claims 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract description 17
- 238000009472 formulation Methods 0.000 abstract description 12
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 12
- 239000007787 solid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 239000005416 organic matter Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 241001509286 Thiobacillus denitrificans Species 0.000 description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
- 239000013618 particulate matter Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241000605118 Thiobacillus Species 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010954 inorganic particle Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Water Supply & Treatment (AREA)
- Genetics & Genomics (AREA)
- Environmental & Geological Engineering (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Hydrology & Water Resources (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a kind of complex microorganism preparations for sewage treatment, and said preparation is by Bdellovibrio Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans ATCC25259.The invention also discloses application of the above-mentioned complex microorganism preparations in terms of removing sewage N, P, S.The present invention is studied and is practiced repeatedly by applicant, it was found that the microorganism formulation that Bdellovibrio Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans ATCC25259 these three bacterial strains are mixed is used directly for sewage treatment, the content of the removal of N, P, K these three elements especially in town sewage, N, P, K these three elements in treated sewage substantially reduces.
Description
Technical field
The invention belongs to sewage treatment field, in particular to a kind of complex microorganism preparations and its system for sewage treatment
Preparation Method.
Background technique
In recent years, due to economic construction fast development, the processing technique of municipal sewage is also constantly progressive, widely used to have
Materializing strategy and two kinds of biological treatment, sludge be a kind of bacterium, fungi, protozoan, inorganic particle composition it is complicated it is non-
Plastid.
Biological reinforcing technology is the high efficiency technical using biological treating waste water, and the mechanism of action is by being added in sewage
Advantage high-efficiency strain increases and improves the ability of processing system.
In existing sewage treatment, be using the organic matter in microorganism decomposition water it is halfway, only will be in sewage
Organic matter decompose on a small quantity, most of organic matter concentrates discharge in form of a sludge, so a large amount of excess sludge is dirty in fact
The concentration discharge for contaminating object can not thoroughly decompose microorganism there is no organic matter is thoroughly decomposed.
Summary of the invention
Goal of the invention: the object of the present invention is to provide a kind of complex microorganism preparations for sewage treatment and its preparations
Method.
Technical solution: a kind of complex microorganism preparations for sewage treatment provided by the invention, said preparation is by Bdellovibrio
Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans ATCC25259.
Total inoculum concentration of three kinds of bacterium is 2 × 107~5 × 107CFU/ml。
Wherein, the inoculum concentration of above-mentioned Bdellovibrio Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans ATCC25259
The ratio between be 1:2:2~3:5:6.
Wherein, above-mentioned complex microorganism preparations growth culture solution ingredient used is glucose 10g/L, (NH4)2SO43g/
L, K2HPO413g/L, KH2PO44g/L, NaCl 6g/L.
Wherein, above-mentioned complex microorganism preparations further include carrier, and the carrier is kaolin, in bentonite, calcium carbonate
It is one or more of.
Wherein, above-mentioned complex microorganism preparations further include one of starch, molasses, trehalose, sucrose, maltose or several
Kind.
Wherein, application of the above-mentioned complex microorganism preparations for sewage treatment in terms of removing sewage N, P, S.
The utility model has the advantages that the present invention is studied and practiced repeatedly by applicant, Bdellovibrio Bdh5221, bacillus subtilis are found
The microorganism formulation that bacterium BL21 and thiobacillus denitrificans ATCC25259 these three bacterial strains are mixed is used directly at sewage
Reason, the removal of N, P, K these three elements especially in town sewage, N, P, K these three elements in treated sewage
Content substantially reduces.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies specific material proportion, process conditions and its result described in example and is merely to illustrate the present invention, without that should will not limit
The present invention described in detail in claims processed.
Bdellovibrio Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans ATCC25259 are commercially available, this hair
The DM culture medium purchase of bright use is in the rich biology in Qingdao sea.
The preparation of 1 microorganism formulation of embodiment
The Bdellovibrio Bdh5221 that inclined-plane saves is added in culture solution, is put into 30 DEG C, the constant temperature water bath of 220r/min shakes
Bed culture 12h, is then added in denitrification culture medium DM culture medium and cultivates, be finally centrifuged 5min at 8000r/min, take precipitating
Host bacteria suspension can be obtained with sterile phosphate buffer resuspension, and adjust its concentration with sterile phosphate buffer and exist
109Cfu/mL obtains Bdellovibrio Bdh5221 bacteria suspension；Same method respectively obtain bacillus subtilis BL21 bacteria suspension and
Thiobacillus denitrificans ATCC25259 bacteria suspension；By 3 kinds of bacteria suspensions according to Bdellovibrio Bdh5221, bacillus subtilis BL21 and denitrogenation
The volume ratio of Thiobacillus ATCC25259 is 1:2:2 mixing, mixes, mixture can be obtained, and total inoculum concentration of three kinds of bacterium is 2
×107CFU/ml；Then kaolin, starch and molasses are added in obtained mixture and obtain microorganism formulation.The culture solution at
It is divided into glucose 10g/L, (NH4)2SO43g/L, K2HPO413g/L, KH2PO44g/L, NaCl 6g/L.
The preparation of 2 microorganism formulation of embodiment
In the same manner as in Example 1, different to be, Bdellovibrio Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans
The volume ratio of ATCC25259 is 3:5:6 mixing, mixes, mixture can be obtained, total inoculum concentrations of three kinds of bacterium is 5 ×
107CFU/ml, the carrier of addition are bentonite and trehalose and sucrose.
The preparation of 3 microorganism formulation of embodiment
In the same manner as in Example 1, different to be, Bdellovibrio Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans
The volume ratio of ATCC25259 is 1:3:4 mixing, mixes, mixture can be obtained, total inoculum concentrations of three kinds of bacterium is 3 ×
107CFU/ml, the carrier of addition are calcium carbonate and maltose and sucrose.
Application of the microorganism formulation of 1 embodiment 1 of experimental example preparation in processing town sewage ability
The sewage of offer is the sewage of certain cities and towns sewers, and the initial concentration of N is 300mg/L in the sewage, and P is initially dense
It is 150mg/L that degree, which is 200mg/L, S initial concentration, and the sewage passes through solid-liquid separator, is separated by solid-liquid separation, and removal bulk is solid
Body particulate matter, subsequent liquid enter sedimentation basin, precipitate 3 hours, then liquid is removed solid floccules by circular hole filter screen,
The Circularhole diameter of circular hole filter screen is 0.09mm.Then embodiment 1 is added according to the amount of 10g/L in treated sewage to be prepared into
The microorganism formulation arrived is handled 3 days.The sewage that detection processing is crossed, discovery N concentration are 15mg/L, and P concentration is 1.5mg/L, S concentration
For 5mg/L.
The microorganism formulation of 2 embodiment 2 of experimental example preparation is handling the application in the sewage ability in urban river
The sewage of offer is the sewage in certain urban river, and the initial concentration of N is 150mg/L, P initial concentration in the sewage
It is 130mg/L for 180mg/L, S initial concentration, the sewage passes through solid-liquid separator, is separated by solid-liquid separation, and bulk solid is removed
Particulate matter, subsequent liquid enter sedimentation basin, precipitate 3 hours, then liquid is removed solid floccules, circle by circular hole filter screen
The Circularhole diameter of hole filter screen is 0.09mm.Then embodiment 1 is added according to the amount of 10g/L in treated sewage to be prepared
Microorganism formulation handle 3 days.The sewage that detection processing is crossed, discovery N concentration are 10mg/L, and P concentration is 1.5mg/L, and S concentration is
5mg/L。
Application of the microorganism formulation of 3 embodiment 3 of experimental example preparation in processing town domestic sewage ability
The sewage of offer is certain town domestic sewage, and the initial concentration of N is 130mg/L in the sewage, and P initial concentration is
150mg/L, S initial concentration are 100mg/L, and the sewage passes through solid-liquid separator, is separated by solid-liquid separation, and remove bulk solid
Grain substance, subsequent liquid enter sedimentation basin, precipitate 3 hours, then liquid is removed solid floccules, circular hole by circular hole filter screen
The Circularhole diameter of filter screen is 0.09mm.Then treated sewage is added what embodiment 1 was prepared according to the amount of 10g/L
Microorganism formulation is handled 3 days.The sewage that detection processing is crossed, discovery N concentration are 9mg/L, and P concentration is 1.3mg/L, and S concentration is
4mg/L。
Claims (2)
1. a kind of complex microorganism preparations for sewage treatment, which is characterized in that said preparation is by Bdellovibrio Bdh5221, withered grass
Bacillus BL21 and thiobacillus denitrificans ATCC25259, total inoculum concentration of three kinds of bacterium are 2 × 107~5×107 CFU/ml, institute
Stating the ratio between inoculum concentration of Bdellovibrio Bdh5221, bacillus subtilis BL21 and thiobacillus denitrificans ATCC25259 is 1:2:2 ~ 3:5:
6, complex microorganism preparations growth culture solution ingredient used is glucose 10g/L, (NH4)2SO43g/L, K2HPO4 13g/
L, KH2PO46 g/L of 4 g/L, NaCl, the complex microorganism preparations further include carrier, the carrier be kaolin, bentonite,
One or more of calcium carbonate, the complex microorganism preparations further include starch, molasses, trehalose, sucrose, in maltose
It is one or more of.
2. application of the complex microorganism preparations described in claim 1 for sewage treatment in terms of removing sewage N, P, S.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610520770.8A CN106085913B (en) | 2016-07-01 | 2016-07-01 | A kind of complex microorganism preparations and preparation method thereof for sewage treatment |
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|---|---|---|---|
| CN201610520770.8A CN106085913B (en) | 2016-07-01 | 2016-07-01 | A kind of complex microorganism preparations and preparation method thereof for sewage treatment |
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| CN106085913A CN106085913A (en) | 2016-11-09 |
| CN106085913B true CN106085913B (en) | 2019-02-22 |
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| CN109928518B (en) * | 2019-04-20 | 2021-07-20 | 浙江友创环境技术有限公司 | Efficient water purifying agent |
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