CN106085410A - PEI modified carbon point FITC complex is as ratio fluorescent pH probe and Cu2+the application of fluorescent probe - Google Patents
PEI modified carbon point FITC complex is as ratio fluorescent pH probe and Cu2+the application of fluorescent probe Download PDFInfo
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Abstract
The invention discloses PEI modified carbon point FITC complex as ratio fluorescent pH probe and Cu2+The application of fluorescent probe.The present invention uses cherry tomato to be raw material, prepares fluorescence by Hydrothermal Synthesis and pH is changed insensitive carbon point, and PEI modifies CDs then, and the amino on PEI reacts with isosulfocyanate radical on FITC molecule, forms complex.The response that in complex, pH is changed by the fluorescence of FITC is high, and ratio probes based on CDs and FITC fluorescence detects for pH；Amino on PEI and Cu2+Coordination is occurred to make the fluorescence intensity of CDs Yu FITC with Cu in solution2+Concentration raises and declines, it is achieved detection Cu2+.The plurality of advantages such as the fluorescent probe selection that the present invention builds is novel, it is highly sensitive to have, selectivity good, simple to operate, range of linearity width, detection limit are low, can be used for pH and Cu in aqueous solution and biological sample2+Efficient detection.
Description
Technical field
The invention belongs to the crossing domain of chemistry, biology and materialogy, relate to material prepare, biological and environment ion inspection
Survey and pH detection, be specifically related to the preparation of PEI modified carbon point-FITC complex and its as ratio fluorescent pH probe and
Cu2+The application of fluorescent probe.
Background technology
Quantum dot typically refers to be smaller in size than or close to the semiconductor nano of its Exciton Bohr Radius, is that a class has solely
The nanoparticle of special optical, electrical characteristic.Compared with one-dimensional or Two-dimensional Carbon nano material, use carbon point prepared by nontoxic biological raw material
(CDs) having higher specific surface area and more edge active site, this makes CDs compare large-size carbon material at bio-sensing
More extensive and efficient application prospect has been possessed with catalytic field.Additionally, the photoluminescent property of CDs is better than repairing without group after amination
The CDs of decorations, and polymine (PEI) can regulate and control its optical property as the nitrogen source of CDs.In addition, PEI is as one
Polyamine family macromolecule has good coordination to metal ion so that it is have in ion detection, biomedicine and imaging field
Having important application, correlational study has become one of current study hotspot.
H in solution+Concentration or pH value are the important parameters characterizing SOLUTION PROPERTIES, and it not only affects other materialization of solution
Matter, also affects response speed and direction simultaneously, therefore, the measurement of pH value industrial and agricultural production, medicine, biological engineering, environment and
The fields such as scientific research are significant.Comparing traditional pH reagent paper and the detection of pH glass electrode, photochemistry pH sensor is then
There is advantages such as can reusing, the time is fast, volume is little, electromagnetism interference performance is strong, pollution-free, all kinds of chemical reactions,
The aspects such as the telemetry and telecommand of environmental monitoring, biomedical sector and the dangerous situation such as underground mine, weapon test have wide
Application prospect.Fluorescein isothiocyanate (FITC) is widely used in biomedical sector as fluorescent probe, and FITC is as glimmering
Light pH indicator its fluorescence intensity in the solution that pH is relatively low is little, and in the solution that pH is higher, its fluorescence intensity increases, detection spirit
Sensitivity is high.
Cu2+In environmental area and biology or chemical system, play vital effect, effectively detect Cu2+
It is necessary.Literature research shows, the CDs that PEI modifies can specifically detect Cu2+, Yongqiang Dong etc. devises one
Plant the fluorescent probe modifying CDs based on PEI, along with Cu2+Concentration increases, and CDs fluorescence intensity increases, and then can be to Cu2+It is fixed to carry out
Measure fixed (Yongqiang Dong, Ruixue Wang, Geli Li, Congqiang Chen, Yuwu Chi, * and Guonan
Chen., Anal.Chem., 2012,84,6220-6224).Goon, I.Y. etc. utilize the Fe that PEI modifies3O4NPs carrys out detection by quantitative
Hg2+(Goon, I.Y., Zhang, C.C., Lim, M.；, Gooding, J.J., Amal, R.Langmuir 2010,26,12247-
12252).Fangkai Du etc. constructs FRET (fluorescence resonance energy transfer) pH sensor, and CDs supplies as Fluorescence Resonance Energy transfer
Body, FITC is energy acceptor, it is achieved that to the Sensitive Detection of pH (Fangkai Du., Yunhao Ming., Fang Zeng.,
Changmin Yu and Shuizhu Wu.,Nanotechnology.,2013,24,365101)。
But above-mentioned detection major part pertains only to the detection of one pack system, and it is many to often relate to detect object in actual application
Component detects, the heavy metal of such as sewage and pH value, generally requires multiple detection probe or detection equipment, so makes detection
Loaded down with trivial details, so, explore a kind of multi-functional detection means and have a good application prospect.
Summary of the invention
For problem of the prior art, it is an object of the invention to provide a kind of multi-functional detection means to detect pH and
Cu2+。
For achieving the above object, the present invention relates to techniques below scheme:
First, the present invention provides a kind of PEI modified carbon point-FITC complex as ratio fluorescent pH probe and Cu2+Fluorescence
The application of probe.
Described PEI modified carbon point-FITC complex: on the basis of PEI modified carbon point, further coupling FITC is prepared into
Arrive；The preparation of PEI modified carbon point belongs to the state of the art, as Yongqiang Dong etc. (Yongqiang Dong,
Ruixue Wang,Geli Li,Congqiang Chen,Yuwu Chi,and Guonan Chen.,Anal.Chem.2012,
84,6220-6224) disclosure, introduces the application in this together.
Preferably in technical scheme, PEI modified carbon point-FITC complex is prepared via a method which: molten to carbon point (CDs)
Adding polymine (PEI) in liquid, mix homogeneously carries out the PEI of carbon point and modifies, and is subsequently adding Fluorescein isothiocyanate
(FITC) carry out coupling, prepare PEI modified carbon point-FITC complex.
In preferred technical scheme, described carbon point (CDs) is prepared via a method which: squeezed the juice by cherry tomato, weigh β-
Cyclodextrin powder adds in holy girl's fruit juice, stirs, autoclave hydro-thermal reaction, reactant centrifugation, collects upper strata dispersion
Liquid, with membrane filtration, removes bulky grain；The filtrate of upper strata dispersion liquid is poured into rotary evaporation in Rotary Evaporators, adds third
Ketone and ethanol extraction, separatory, centrifugation, lower sediment thing is carbon point (CDs).
Although about pH and Cu2+The preparation method of fluorescent probe existing document report, but so far, not yet have based on
PEI modified carbon point-FITC composite is simultaneously used for detecting pH and Cu2+Domestic and foreign literature and patent report.The present invention
On the basis of early-stage Study, find that PEI modified carbon point-FITC complex serves not only as ratio fluorescent pH probe and carries out pH
Detection, and double fluorescent quenching based on CDs Yu FITC, can effectively detect Cu2+Concentration.Concrete, the present invention utilizes
Amino on PEI, can not only be used for amination and modifies CDs, can react with isosulfocyanate radical on FITC by specificity again.Work as pH of buffer
Time different, CDs fluorescence intensity is almost unchanged, therefore can be as reference, and FITC fluorescence intensity gradually rises with pH and increases, so
CDs Yu FITC ratio fluorescent can be used for qualitative detection pH value of solution；The coupling of FITC Yu PEI modified carbon point, does not only interfere with PEI
With Cu2+Coordination combine, and based on PEI and Cu2+Combination can cause the double fluorescence of CDs Yu FITC combined respectively at PEI
Quenching effect, promotes detection signal, works as Cu2+In the presence of, Cu2+Can be specific binding with PEI, with Cu2+Concentration raises so that
CDs with FITC fluorescence intensity decreases simultaneously, causes fluorescent quenching, matching system CDs and FITC fluorescence intensity and Cu2+Concentration
Between relation, can build and detect pH and Cu based on CDs-FITC complex simultaneously2+Fluoroscopic examination means.
Secondly, the present invention provides a kind of detection pH and Cu2+Detection method, the method includes:
PEI modified carbon point-FITC complex is used for pH detection: PEI modified carbon point-FITC complex is slow from different pH's
Rush liquid mixing, measure the ratio fluorescent intensity I of systemFITC/ICDs(IFITCFor FITC fluorescence intensity in the buffer of different pH
Value, ICDsFor CDs fluorescence intensity level in the buffer of different pH), matching system ratio fluorescent intensity and the relation of pH, enter
The detection of row pH value of solution to be measured；
PEI modified carbon point-FITC complex is used for Cu2+Detection: by the PEI modified carbon point identical pH of-FITC complex
Buffer dilution, measure system fluorescence intensity, be subsequently adding Cu2+Hatching, then measure the fluorescence intensity of system, matching system
CDs Yu FITC fluorescence intensity and Cu2+Relation between concentration, carries out solution C u to be measured2+The detection of concentration.
Described PEI modified carbon point-FITC complex: on the basis of PEI modified carbon point, further coupling FITC is prepared into
Arrive, it is preferred that PEI modified carbon point-FITC complex is prepared via a method which: in carbon point (CDs) solution, add polyethylene
Imines (PEI), mix homogeneously carries out the PEI of carbon point and modifies, is subsequently adding Fluorescein isothiocyanate (FITC) and carries out coupling, prepare
PEI modified carbon point-FITC complex.
Preferably in technical scheme, described carbon point (CDs) is prepared via a method which: is squeezed the juice by cherry tomato, weighs β-ring
Dextrin powder adds in holy girl's fruit juice, stirs, autoclave hydro-thermal reaction, reactant centrifugation, collects upper strata dispersion liquid,
With membrane filtration, remove bulky grain；The filtrate of upper strata dispersion liquid is poured into rotary evaporation in Rotary Evaporators, add acetone and
Ethanol extracts, and separatory, centrifugation, lower sediment thing is carbon point (CDs).
Concrete, cherry tomato is squeezed the juice and is placed in clean beaker, weigh a certain amount of beta-schardinger dextrin-powder and add holy girl
In fruit juice, by magnetic agitation, autoclave hydro-thermal reaction, centrifugation, collect upper strata dispersion liquid, with membrane filtration, remove big
Granule, pours in Rotary Evaporators by the filtrate of upper strata dispersion liquid, at a certain temperature rotary evaporation in flask without bright
Aobvious liquid, adds acetone and ethanol extraction, separatory, centrifugation, repeat this step 3 time and more than, lower sediment thing is carbon
Point (CDs).
The present invention uses cherry tomato and beta-schardinger dextrin-hydro-thermal method to prepare carbon point, explores new carbon point and prepares material, made
Standby carbon point has excellent optical property, and quantum yield is high, and its fluorescence intensity is suitable to the need of relative broad range pH detection stability
Want, and this preparation method technique is simple, efficiently, raw material is nontoxic, be easy to get.
The present invention provides a kind of detection pH and Cu2+The construction method of the detection system of concentration, comprises the steps:
1) cherry tomato is squeezed the juice it is placed in beaker, weigh beta-schardinger dextrin-powder and add in holy girl's fruit juice, stirred by magnetic force
Mix, autoclave hydro-thermal reaction, centrifugation, collect upper strata dispersion liquid, with membrane filtration, remove bulky grain, by upper strata dispersion liquid
Filtrate pour in Rotary Evaporators, without obvious liquid in rotary evaporation to flask, add acetone and ethanol extraction, separatory,
Centrifugation, repeat this step 3 time and more than, lower sediment thing is carbon point (CDs)；
2) in the CDs solution of preparation, add polymine (PEI), magnetic agitation under room temperature, add isothiocyanic acid glimmering
Light element (FITC), continues magnetic agitation, prepares PEI modified carbon point-FITC complex；
3) the PEI modified carbon point-FITC complex of preparation is divided into two parts standby,
Part I PEI modified carbon point-FITC complex for pH detection system build: by CDs-FITC complex with not
Mix with the phosphate buffer of pH, measure the ratio fluorescent intensity (I of systemFITC/ICDs), matching system ratio fluorescent intensity
With the relation of pH, build pH ratio fluorescent probe in detecting system based on PEI modified carbon point-FITC complex；
Part II PEI modified carbon point-FITC complex is used for Cu2+Detection system builds: used by CDs-FITC complex
The phosphate buffer dilution of identical pH, measures system fluorescence intensity, is subsequently adding variable concentrations Cu2+Hatching, then measure system
Fluorescence intensity, matching system fluorescence intensity and Cu2+Relation between concentration, builds based on PEI modified carbon point-FITC complex
Cu2+Fluorescent probe detection system.
Preferably in technical scheme, the concrete technology condition of step (1) is: the volume of holy girl's fruit juice is 10～100mL, β-
The quality of cyclodextrin powder is 0.1～1.0g, and the time of magnetic agitation is 1～10min, and hydrothermal temperature is 100～200
DEG C, the hydro-thermal reaction time is 2～12h, the rotating speed of the centrifugation after hydro-thermal reaction and time is respectively 5000～16000rpm,
3～15min, the temperature set in Rotary Evaporators is as 25～80 DEG C, and the volume adding acetone is 5～50mL, adds ethanol
Volume is 5～50mL, and the rotating speed of the centrifugation after separatory and time are respectively 5000～16000rpm, 3～15min.
Preferably in technical scheme, the concrete technology condition of step (2) is: CDs concentration and volume be respectively 0.1～
1.0mgmL-1, 1～10mL, PEI mass fraction and volume be respectively 0.01～0.1%, 0.1～1.0mL, the magnetic agitation time is
30min～6h, FITC concentration and volume are respectively 0.01～0.1mM, 5～50mL, and the magnetic agitation time is 30min～3h.
Preferably in technical scheme, the concrete technology condition of step (3) is: Part I PEI modified carbon point-FITC is combined
The volume of thing is 20～200 μ L, and the volume of 10mM phosphate buffered solution is 1～10mL, the pH of phosphate buffer be 3.2～
9.0；The volume of Part II PEI modified carbon point-FITC composite is 20～200 μ L, phosphate buffered solution volume
Being 1～10mL, the pH of phosphate buffer is 7.4, Cu2+Concentration be 0.01～10.0 μM, brooding time 1～10min.
The present invention achieves following beneficial effect:
(1) present invention discover that PEI modified carbon point-FITC complex serves not only as ratio fluorescent pH probe and carries out pH's
Detection, and double fluorescent quenching based on CDs Yu FITC, can effectively detect Cu2+Concentration, provides a kind of multi-functional for this
Detection means, and in order to expand the H of FITC+Sensor range of application, increase sensor service life and improve sensitive
Degree, the present invention devises ratio fluorescent sensor based on CDs-FITC and detects pH and Cu for simultaneously2+。
(2) present invention uses new carbon point to prepare material, and prepared carbon point has excellent optical property, and it is wider
Scope pH is stable, passes through IFITC/ICDsThe detection of ratio fluorescent intensity, can improve Detection accuracy and detection efficiency.
(3) the inventive method mild condition, equipment is simple, easily operated, CDs-FITC complex can pass through Hydrothermal Synthesis
Method, rotary evaporation purifies and directly prepares, and raw material availability is high, low cost.
(4) the invention provides that a kind of technique is simple, productivity is high, production cost is low, environmental protection, be easy to operate and control
A kind of based on carbon point/Fluorescein isothiocyanate complex ratio fluorescent pH probe and Cu2+The system of fluorescent probe detection system
Preparation Method, compared with prior art, probe in detecting system of the present invention has highly sensitive, detection range width, and detection limit is low etc. excellent
Point, having a extensive future of the fields such as biochemical analysis, medical diagnosis, environmental monitoring.
Accompanying drawing explanation
Fig. 1 ratio fluorescent based on carbon point/Fluorescein isothiocyanate complex pH probe and Cu2+The preparation side of fluorescent probe
Method and principle schematic thereof；
Fig. 2: PEI modified carbon point-FITC complex fluorescence emission spectrum under condition of different pH；
The ratio fluorescent intensity of Fig. 3: PEI modified carbon point-FITC complex and the Linear Fit Chart of corresponding pH；
Fig. 4: PEI modified carbon point-FITC complex is at variable concentrations Cu2+In the presence of fluorescence emission spectrogram；
FITC and CDs relative intensity of fluorescence and corresponding Cu in Fig. 5: PEI modified carbon point-FITC complex2+Line under concentration
Property fitted figure.
Detailed description of the invention
Mode by the following examples further illustrates the present invention, but the most therefore limits the present invention to described reality
Execute among example scope.The experimental technique of unreceipted actual conditions in the following example, conventionally selects with condition
Select.
Embodiment 1
Use juice extractor to squeeze fresh holy girl fruit juice 30mL, be placed in clean beaker；Weigh 0.5g beta-schardinger dextrin-powder
Add in holy girl's fruit juice, magnetic agitation 5min, transposition autoclave hydro-thermal reaction 5h, reaction temperature 120 DEG C；Reaction is centrifugal after terminating
Separating (10000rpm, 15min), collect upper strata dispersion liquid, filter membrane filters, and removes bulky grain；Upper strata dispersion liquid is poured into
In Rotary Evaporators, at a temperature of 40 DEG C rotary evaporation in flask without obvious liquid, be separately added into acetone and the second of 20mL
Alcohol, centrifugation (12000rpm, 5min)；Repeating this centrifugation step three times, lower sediment thing is CDs；Preparation 1.0mg mL- 1CDs aqueous solution 10mL, adds the PEI 0.2mL of 0.1%, magnetic agitation 0.5h under room temperature；Add the FITC 10mL of 0.05mM
Continue magnetic agitation 1h and prepare PEI modified carbon point-FITC complex.
The complex of above-mentioned preparation is divided into two parts: a part detects for pH, the PEI modified carbon point-FITC of 200 μ L
The 10mM phosphate buffer mixing of complex pH different from 3mL (3.2～9.0)；The fluorescence intensity of measurement system, matching system
Ratio fluorescent intensity and the relation of pH, build pH ratio fluorescent probe in detecting body based on PEI modified carbon point-FITC complex
System.It is additionally useful for Cu2+Detection, by 200 μ L PEI modified carbon point-FITC complex with 3mL phosphate buffer (10mM,
PH7.4) dilution, measures the fluorescence intensity of system, is subsequently adding 0.01～10.0 μM of Cu2+Hatching 10min, then measure system
Fluorescence intensity, matching system fluorescence intensity and Cu2+Relation between concentration, builds based on PEI modified carbon point-FITC complex
Cu2+Fluorescent probe detection system.
Embodiment 2
Use juice extractor to squeeze fresh holy girl fruit juice 50mL, be placed in clean beaker；Weigh 0.7g beta-schardinger dextrin-powder
Add in holy girl's fruit juice, magnetic agitation 7min, transposition autoclave hydro-thermal reaction 3h, reaction temperature 140 DEG C；Reaction is centrifugal after terminating
Separating (12000rpm, 10min), collect upper strata dispersion liquid, filter membrane filters, and removes bulky grain；Upper strata dispersion liquid is poured into
In Rotary Evaporators, at a temperature of 60 DEG C rotary evaporation in flask without obvious liquid, be separately added into acetone and the second of 30mL
Alcohol, centrifugation (12000rpm, 10min)；Repeating this centrifugation step three times, lower sediment thing is CDs；Preparation 0.5mg
mL-1CDs aqueous solution 10mL, adds the PEI 0.1mL of 0.1%, magnetic agitation 1h under room temperature；The FITC 5mL adding 0.05mM continues
Continuous magnetic agitation 2h prepares PEI modified carbon point-FITC complex.
The complex of above-mentioned preparation is divided into two parts: a part detects for pH, the PEI modified carbon point-FITC of 100 μ L
The 10mM phosphate buffer mixing of complex pH different from 2mL (3.2～9.0)；The fluorescence intensity of measurement system, matching system
Ratio fluorescent intensity and the relation of pH, build pH ratio fluorescent probe in detecting body based on PEI modified carbon point-FITC complex
System.It is additionally useful for Cu2+Detection, dilutes 100 μ L CDs-FITC complex 5mL phosphate buffer (10mM, pH7.4), surveys
The fluorescence intensity of amount system, is subsequently adding 0.01～10.0 μM of Cu2+Hatching 10min, then measure the fluorescence intensity of system, matching
System fluorescence intensity and Cu2+Relation between concentration, builds Cu based on PEI modified carbon point-FITC complex2+Fluorescent probe is examined
Survey system.
Embodiment 3
Use juice extractor to squeeze fresh holy girl fruit juice 80mL, be placed in clean beaker；Weigh 0.8g beta-schardinger dextrin-powder
Add in holy girl's fruit juice, magnetic agitation 10min, transposition autoclave hydro-thermal reaction 6h, reaction temperature 140 DEG C；Reaction terminate after from
The heart separates (12000rpm, 10min), collects upper strata dispersion liquid, and filter membrane filters, and removes bulky grain；Upper strata dispersion liquid is fallen
Enter in Rotary Evaporators, at a temperature of 60 DEG C rotary evaporation in flask without obvious liquid, be separately added into 20mL acetone and
Ethanol, centrifugation (12000rpm, 10min)；Repeating this centrifugation step three times, lower sediment thing is CDs；Preparation 0.5mg
mL-1CDs aqueous solution 10mL, adds the PEI 0.2mL of 0.1%, magnetic agitation 1h under room temperature；The FITC 5mL adding 0.05mM continues
Continuous magnetic agitation 2h prepares PEI modified carbon point-FITC complex.
The complex of above-mentioned preparation is divided into two parts: a part detects for pH, the PEI modified carbon point-FITC of 150 μ L
The 10mM phosphate buffer mixing of complex pH different from 5mL (3.2～9.0)；The fluorescence intensity of measurement system, matching system
Ratio fluorescent intensity and the relation of pH, build pH ratio fluorescent probe in detecting body based on PEI modified carbon point-FITC complex
System.It is additionally useful for Cu2+Detection, by 150 μ L PEI modified carbon point-FITC complex with 5mL phosphate buffer (10mM,
PH7.4) dilution, measures the fluorescence intensity of system, is subsequently adding 0.01～10.0 μM of Cu2+Hatching 10min, then measure system
Fluorescence intensity, matching system fluorescence intensity and Cu2+Relation between concentration, builds based on PEI modified carbon point-FITC complex
Cu2+Fluorescent probe detection system.
Above-mentioned detection probe is 4.6～7.7 for the pH detection range of linearity；Cu2+Detection is in the range of 0.01～12.45 μM
It is 7nM with Monitoring lower-cut.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1.PEI modified carbon point-FITC complex is as ratio fluorescent pH probe and Cu2+The application of fluorescent probe, described PEI modifies
Carbon point-FITC complex is on the basis of PEI modified carbon point, the complex that further coupling FITC prepares.
Application the most according to claim 1, it is characterised in that PEI modified carbon point-FITC complex is made by the following method
Standby: to add polymine (PEI) in carbon point (CDs) solution, mix homogeneously carries out the PEI of carbon point and modifies, and is subsequently adding different
Hydrogen thiocyanate fluorescein (FITC) carries out coupling, prepares PEI modified carbon point-FITC complex.
Application the most according to claim 1 and 2, it is characterised in that carbon point (CDs) is prepared via a method which: by holy girl
Fruit is squeezed the juice, and weighs beta-schardinger dextrin-powder and adds in holy girl's fruit juice, stirs, autoclave hydro-thermal reaction, reactant centrifugation,
Collect upper strata dispersion liquid, with membrane filtration, remove bulky grain；The filtrate of upper strata dispersion liquid is poured in Rotary Evaporators and rotate
Evaporation, adds acetone and ethanol extraction, and separatory, centrifugation, lower sediment thing is carbon point (CDs).
4. detection pH and Cu2+Detection method, the method includes:
Described PEI modified carbon point-FITC complex is on the basis of PEI modified carbon point, and further coupling FITC prepares
Complex,
PEI modified carbon is selected-FITC complex and is used for pH detection: PEI modified carbon selects the buffer of-FITC complex and different pH
Mixing, measures the ratio fluorescent intensity I of systemFITC/ICDs, matching system ratio fluorescent intensity and the relation of pH, carry out to be measured molten
The detection of liquid pH；
PEI modified carbon point-FITC complex is used for Cu2+Detection: by PEI modified carbon point-FITC complex delaying with identical pH
Rush liquid dilution, measure system fluorescence intensity, be subsequently adding Cu2+Hatching, then measure the fluorescence intensity of system, matching system fluorescence is strong
Degree and Cu2+Relation between concentration, carries out solution C u to be measured2+The detection of concentration.
Method the most according to claim 4, it is characterised in that PEI modified carbon point-FITC complex is made by the following method
Standby: to add polymine (PEI) in carbon point (CDs) solution, mix homogeneously carries out the PEI of carbon point and modifies, and is subsequently adding different
Hydrogen thiocyanate fluorescein (FITC) carries out coupling, prepares PEI modified carbon point-FITC complex.
6. according to the method described in claim 4 or 5, it is characterised in that carbon point (CDs) is prepared via a method which: by holy girl
Fruit is squeezed the juice, and weighs beta-schardinger dextrin-powder and adds in holy girl's fruit juice, stirs, autoclave hydro-thermal reaction, reactant centrifugation,
Collect upper strata dispersion liquid, with membrane filtration, remove bulky grain；The filtrate of upper strata dispersion liquid is poured in Rotary Evaporators and rotate
Evaporation, adds acetone and ethanol extraction, and separatory, centrifugation, lower sediment thing is carbon point (CDs).
7. detection pH and Cu2+The construction method of the detection system of concentration, comprises the steps:
1) cherry tomato is squeezed the juice it is placed in beaker, weigh beta-schardinger dextrin-powder and add in holy girl's fruit juice, by magnetic agitation, high
Pressure still hydro-thermal reaction, centrifugation, collect upper strata dispersion liquid, with membrane filtration, remove bulky grain, by the filtration of upper strata dispersion liquid
Liquid is poured in Rotary Evaporators, without obvious liquid in rotary evaporation to flask, adds acetone and ethanol extraction, separatory, is centrifuged and divides
From, repeat this step 3 time and more than, lower sediment thing is carbon point (CDs)；
2) in the CDs solution of preparation, add polymine (PEI), magnetic agitation under room temperature, add Fluorescein isothiocyanate
(FITC), continue magnetic agitation, prepare PEI modified carbon point-FITC complex；
3) the PEI modified carbon point-FITC complex of preparation is divided into two parts standby,
Part I PEI modified carbon point-FITC complex builds for pH detection system: by CDs-FITC complex and different pH
Phosphate buffer mixing, measure system ratio fluorescent intensity (IFITC/ICDs), matching system ratio fluorescent intensity and pH
Relation, build pH ratio fluorescent probe in detecting system based on PEI modified carbon point-FITC complex；
Part II PEI modified carbon point-FITC complex is used for Cu2+Detection system builds: by CDs-FITC complex with identical
The phosphate buffer dilution of pH, measures system fluorescence intensity, is subsequently adding variable concentrations Cu2+Hatching, then measure the glimmering of system
Light intensity, matching system fluorescence intensity and Cu2+Relation between concentration, builds Cu based on PEI modified carbon point-FITC complex2+
Fluorescent probe detection system.
Method the most according to claim 7, it is characterised in that the concrete technology condition of step (1) is: the body of holy girl's fruit juice
Long-pending is 10～100mL, and the quality of beta-schardinger dextrin-powder is 0.1～1.0g, and the time of magnetic agitation is 1～10min, hydro-thermal reaction
Temperature is 100～200 DEG C, and the hydro-thermal reaction time is 2～12h, and after hydro-thermal reaction, rotating speed and the time of centrifugation are respectively
5000～16000rpm, 3～15min, the temperature set in Rotary Evaporators as 25～80 DEG C, add acetone volume be 5～
50mL, the volume adding ethanol is 5～50mL, and after separatory, rotating speed and the time of centrifugation are respectively 5000～16000rpm, 3
～15min.
Method the most according to claim 7, it is characterised in that the concrete technology condition of step (2) is: CDs concentration and body
Long-pending respectively 0.1～1.0mg mL-1, 1～10mL, PEI mass fraction and volume be respectively 0.01～0.1%, 0.1～1.0mL,
The magnetic agitation time is 30min～6h, and FITC concentration and volume are respectively 0.01～0.1mM, 5～50mL, magnetic agitation time
For 30min～3h.
Method the most according to claim 7, it is characterised in that the concrete technology condition of step (3) is: Part I PEI
The volume of modified carbon point-FITC complex is 20～200 μ L, and the volume of 10mM phosphate buffered solution is 1～10mL, phosphate
The pH of buffer is 3.2～9.0；The volume of Part II PEI modified carbon point-FITC composite is 20～200 μ L, phosphorus
Hydrochlorate volume of buffer solution is 1～10mL, and the pH of phosphate buffer is 7.4, Cu2+Concentration be 0.01～10.0 μM, hatching
Time 1～10min.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970061A (en) * | 2017-05-10 | 2017-07-21 | 青岛大学 | The preparation method of carbon point/copper nano-cluster compound ratio fluorescent dopamine probe |
| CN107011891A (en) * | 2017-04-24 | 2017-08-04 | 华东师范大学 | A kind of Cu+Fluorescence probe and its preparation method and application |
| CN107253961A (en) * | 2017-06-29 | 2017-10-17 | 湖南科技大学 | It is a kind of can ratio test cysteine water soluble fluorescence sensor preparation and application |
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| DE102019124795A1 (en) * | 2019-09-16 | 2021-03-18 | Abberior GmbH | Optical pH sensor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110123456A1 (en) * | 2008-03-20 | 2011-05-26 | National University Of Ireland, Galway | Hollow biodegradable nanospheres and nanoshells for delivery of therapeutic and/or imaging molecules |
| CN103536936A (en) * | 2013-10-12 | 2014-01-29 | 上海师范大学 | Carbon nano-tube composite material with fluorescent/ultrasonic dual-mode targeted imaging function, and preparation method and application thereof |
| CN103893777A (en) * | 2014-03-14 | 2014-07-02 | 东华大学 | Preparation method of hyaluronic acid targeted carbon nano-tube loaded anti-cancer medicine |
-
2016
- 2016-06-15 CN CN201610429533.0A patent/CN106085410B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110123456A1 (en) * | 2008-03-20 | 2011-05-26 | National University Of Ireland, Galway | Hollow biodegradable nanospheres and nanoshells for delivery of therapeutic and/or imaging molecules |
| CN103536936A (en) * | 2013-10-12 | 2014-01-29 | 上海师范大学 | Carbon nano-tube composite material with fluorescent/ultrasonic dual-mode targeted imaging function, and preparation method and application thereof |
| CN103893777A (en) * | 2014-03-14 | 2014-07-02 | 东华大学 | Preparation method of hyaluronic acid targeted carbon nano-tube loaded anti-cancer medicine |
Non-Patent Citations (1)
| Title |
|---|
| 刘慧: "荧光碳点的制备及其在药物分析中的应用", 《中国博士学位论文全文数据库（医药卫生科技辑）》 * |
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| CN107011891B (en) * | 2017-04-24 | 2020-01-07 | 华东师范大学 | Cu+Fluorescent probe and preparation method and application thereof |
| CN106970061B (en) * | 2017-05-10 | 2019-04-02 | 青岛大学 | Carbon dots/copper nano-cluster compound ratio fluorescent dopamine probe preparation method |
| CN106970061A (en) * | 2017-05-10 | 2017-07-21 | 青岛大学 | The preparation method of carbon point/copper nano-cluster compound ratio fluorescent dopamine probe |
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| CN110079160B (en) * | 2019-05-29 | 2022-09-09 | 苏州大学 | Fluorescent anti-counterfeiting ink based on ternary triple energy transfer, and preparation method and application thereof |
| DE102019124795A1 (en) * | 2019-09-16 | 2021-03-18 | Abberior GmbH | Optical pH sensor |
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