CN106075574A - A kind of preparation method of the gel stent of repairing articular cartilage damage - Google Patents
A kind of preparation method of the gel stent of repairing articular cartilage damage Download PDFInfo
- Publication number
- CN106075574A CN106075574A CN201610577602.2A CN201610577602A CN106075574A CN 106075574 A CN106075574 A CN 106075574A CN 201610577602 A CN201610577602 A CN 201610577602A CN 106075574 A CN106075574 A CN 106075574A
- Authority
- CN
- China
- Prior art keywords
- sodium
- articular cartilage
- gel
- prepare
- hyaluronate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
Abstract
The invention discloses the preparation method of the gel stent of a kind of repairing articular cartilage damage, comprise the following steps: S1: preparing weight portion is the hyaluronate sodium of 58 parts and the deionized water of 20 25 parts, hyaluronate sodium and deionized water are mixed, at 10 15 DEG C, react 11 14h, prepare sodium hyaluronate solution；S2: preparing weight portion is the PLGA nano-particle of 69 parts and the azo-bis-isobutyrate hydrochloride of 47 parts, PLGA nano-particle is added in sodium hyaluronate solution, 10 15min are reacted at 15 20 DEG C, it is subsequently adding azo-bis-isobutyrate hydrochloride, at 20 25 DEG C, react 20 30min, prepare hyaluronic acid sodium gel complex solution；S3: be injected into by hyaluronic acid sodium gel complex solution at articular cartilage damage to be repaired, then irradiates through ultraviolet point source, obtains the gel stent of repairing articular cartilage damage.The present invention can the most quickly prepare the gel stent of compound joint cartilage injury, and method is simple, easy to use, low cost.
Description
Technical field
The present invention relates to medicine and technical field of medical instruments, the gel particularly relating to the damage of a kind of repairing articular cartilage props up
The preparation method of frame.
Background technology
Along with China enters aging society, knee articular cartilage defect patient gets more and more；At present this type of disease be there is no
Good Therapeutic Method；Subchondral bone punching the most conventional, bone cartilage transplantation and native cartilage cell transplantation can only
Part relief of symptoms, it is impossible to reach the purpose of cartilage regeneration defect, the repair tissue using this type of method to obtain is mainly composed of I
Collagen type, differs greatly compared with the II collagen type of original hyaline cartilage on biological characteristics.
Regeneration and Repair tissue at cartilage defect, the ultimate principle of cartilage regeneration defect is: outside raising self or introducing
The stem cell of source property, relatively germinal cell are at cartilage defect, and are divided into cartilaginous tissue under the effects such as relevant growth factors；Close
Joint chamber there is polytype mescenchymal stem cell can be enrolled at cartilage defect and carries out injury repairing, such as medulla mesenchyma
Synovial Mesenchymal Stem Cells in stem cell, knuckle synovia and the fat mesenchymal stem cell etc. in subpatellar fat pad source；Body
Liquid and blood etc. all have inducing mesenchymal stem cell to orient the ability migrated, and the various factors in cartilage microenvironment have and lure
Lead the ability of mesenchymal stem cells into chondrocytes directed differentiation；But, due to the office of self Immune inducing in vivo factor stress efficacy
The three-dimensional space environment that sex-limited and shortage cell grows, thus, it is impossible to produce the repair tissue similar with original cartilaginous tissue,
In order to solve this difficult problem of regenerating bone or cartilage, domestic and international medical circle has carried out substantial amounts of exploration and research between the past few decades；As
Study various somatomedin to promote that mesenchymal stem cells into chondrocytes breaks up；Research Gene transfer techniques obtains into cartilage
The seed cell that ability is higher；Study novel tissue engineering bracket build organization engineered cartilage in vitro and then implant
Deng；But, all there is various limitation in these methods: somatomedin can not internal use due to immunogenicity, gene transfection skill
There is safety and oncogenicity problem in art, organization engineered cartilage is implanted and is difficult to integration etc. with original tissue；Thus, at present these
Method the most well solves problem；In recent years, at cartilage defect, implantation can raise and induce self mescenchymal stem cell
It it is the therapeutic modality having bright prospects to the three-dimensional rack of Chondrocyte Differentiation；Implant three-dimensional porous rack especially to implant and can delay
The timbering material of release thing is because recruiting more mescenchymal stem cell and strengthen induction usefulness, thus treatment regenerating bone or cartilage brings
Huge hope, has a social and economic significance widely.
At present, research applies more timbering material mostly to be PLGA, PGA, collagen protein, fibrin etc., these supports
Material characteristics is the energy inducing cell substantial amounts of I-type collagen of generation, rather than II collagen type, thus it is unfavorable for that mesenchyme is done
Cell is to Chondrocyte Differentiation；And in the research field of medicine slow release stent, be used for mostly being somatomedin by the medicine of slow release, this
Class drug price is expensive, and has immunogenicity, and vivo applications has the risk bringing zoonosis；Thus, search out
Suitable form, timbering material that emiocytosis cartilage cell epimatrix can be promoted and safely and effectively, can internal use
Induced drug is the key solving regenerating bone or cartilage problem.
Summary of the invention
The technical problem existed based on background technology, the present invention proposes the gel stent of a kind of repairing articular cartilage damage
Preparation method.
The preparation method of the gel stent of a kind of repairing articular cartilage damage that the present invention proposes, comprises the following steps:
S1: prepare hyaluronate sodium and the deionized water of 20-25 part that weight portion is 5-8 part, by hyaluronate sodium and deionization
Water mixes, and reacts 11-14h, prepare sodium hyaluronate solution at 10-15 DEG C；
S2: prepare PLGA nano-particle and the azo-bis-isobutyrate hydrochloride of 4-7 part that weight portion is 6-9 part, by PLGA nanometer
Granule adds in sodium hyaluronate solution, reacts 10-15min, be subsequently adding azo-bis-isobutyrate hydrochloride at 15-20 DEG C,
At 20-25 DEG C, react 20-30min, prepare hyaluronic acid sodium gel complex solution；
S3: hyaluronic acid sodium gel complex solution is injected at articular cartilage damage to be repaired, then through ultraviolet point source
Irradiate, obtain the gel stent of repairing articular cartilage damage.
Preferably, in described S1, prepare hyaluronate sodium and the deionized water of 21-24 part that weight portion is 6-7 part, will thoroughly
Bright matter acid sodium and deionized water mixing, react 12-13h at 11-14 DEG C, prepare sodium hyaluronate solution.
Preferably, in described S2, prepare PLGA nano-particle and azo two 2,2-Dimethylaziridine of 5-6 part that weight portion is 7-8 part
Hydrochlorate, adds PLGA nano-particle in sodium hyaluronate solution, reacts 11-14min, be subsequently adding idol at 16-19 DEG C
Nitrogen two 2,2-Dimethylaziridine hydrochlorate, reacts 22-28min at 21-24 DEG C, prepares hyaluronic acid sodium gel complex solution.
Preferably, in described S3, hyaluronic acid sodium gel complex solution is filled to articular cartilage damage to be repaired,
Then irradiate through ultraviolet point source, obtain the gel stent of repairing articular cartilage damage.
In the present invention, the injection of the preparation method of the gel stent of described a kind of repairing articular cartilage damage is modified transparent
Matter acid sodium gel, for efficient cross-linked gel, dosage of crosslinking agent is few, has stability and the cohesion of enhancing, has the stream of excellence
Becoming and learn performance, plasticity is good and is prone to injection, and internal retention time is long, and filling effect is good, and remains to keep biocompatibility,
The present invention can the most quickly prepare the gel stent of compound joint cartilage injury, and method is simple, easy to use, low cost.
Accompanying drawing explanation
The present invention will be further described in detail with specific implementation method below in conjunction with the accompanying drawings.
Fig. 1 is the structural representation of the preparation method of the gel stent of a kind of repairing articular cartilage of present invention damage.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is explained orally further.
Embodiment one
The present embodiment proposes the preparation method of the gel stent of a kind of repairing articular cartilage damage, comprises the following steps:
S1: preparing weight portion is the hyaluronate sodium of 5 parts and the deionized water of 20 parts, hyaluronate sodium and deionized water is mixed
Close, at 10 DEG C, react 11h, prepare sodium hyaluronate solution；
S2: preparing weight portion is the PLGA nano-particle of 6 parts and the azo-bis-isobutyrate hydrochloride of 4 parts, by PLGA nano-particle
Add in sodium hyaluronate solution, at 15 DEG C, react 10min, be subsequently adding azo-bis-isobutyrate hydrochloride, anti-at 20 DEG C
Answer 20min, prepare hyaluronic acid sodium gel complex solution；
S3: hyaluronic acid sodium gel complex solution is injected at articular cartilage damage to be repaired, then through ultraviolet point source
Irradiate, obtain the gel stent of repairing articular cartilage damage.
Embodiment two
The present embodiment proposes the preparation method of the gel stent of a kind of repairing articular cartilage damage, comprises the following steps:
S1: preparing weight portion is the hyaluronate sodium of 6 parts and the deionized water of 23 parts, hyaluronate sodium and deionized water is mixed
Close, at 13 DEG C, react 12h, prepare sodium hyaluronate solution；
S2: preparing weight portion is the PLGA nano-particle of 7 parts and the azo-bis-isobutyrate hydrochloride of 5 parts, by PLGA nano-particle
Add in sodium hyaluronate solution, at 18 DEG C, react 13min, be subsequently adding azo-bis-isobutyrate hydrochloride, anti-at 23 DEG C
Answer 25min, prepare hyaluronic acid sodium gel complex solution；
S3: hyaluronic acid sodium gel complex solution is injected at articular cartilage damage to be repaired, then through ultraviolet point source
Irradiate, obtain the gel stent of repairing articular cartilage damage.
Embodiment three
The present embodiment proposes the preparation method of the gel stent of a kind of repairing articular cartilage damage, comprises the following steps:
S1: preparing weight portion is the hyaluronate sodium of 8 parts and the deionized water of 25 parts, hyaluronate sodium and deionized water is mixed
Close, at 15 DEG C, react 14h, prepare sodium hyaluronate solution；
S2: preparing weight portion is the PLGA nano-particle of 9 parts and the azo-bis-isobutyrate hydrochloride of 7 parts, by PLGA nano-particle
Add in sodium hyaluronate solution, at 20 DEG C, react 15min, be subsequently adding azo-bis-isobutyrate hydrochloride, anti-at 25 DEG C
Answer 30min, prepare hyaluronic acid sodium gel complex solution；
S3: hyaluronic acid sodium gel complex solution is injected at articular cartilage damage to be repaired, then through ultraviolet point source
Irradiate, obtain the gel stent of repairing articular cartilage damage.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope that the invention discloses, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (4)
1. the preparation method of the gel stent of a repairing articular cartilage damage, it is characterised in that comprise the following steps:
S1: prepare hyaluronate sodium and the deionized water of 20-25 part that weight portion is 5-8 part, by hyaluronate sodium and deionization
Water mixes, and reacts 11-14h, prepare sodium hyaluronate solution at 10-15 DEG C；
S2: prepare PLGA nano-particle and the azo-bis-isobutyrate hydrochloride of 4-7 part that weight portion is 6-9 part, by PLGA nanometer
Granule adds in sodium hyaluronate solution, reacts 10-15min, be subsequently adding azo-bis-isobutyrate hydrochloride at 15-20 DEG C,
At 20-25 DEG C, react 20-30min, prepare hyaluronic acid sodium gel complex solution；
S3: hyaluronic acid sodium gel complex solution is injected at articular cartilage damage to be repaired, then through ultraviolet point source
Irradiate, obtain the gel stent of repairing articular cartilage damage.
The preparation method of the gel stent of a kind of repairing articular cartilage the most according to claim 1 damage, it is characterised in that
In described S1, preparing weight portion is hyaluronate sodium and the deionized water of 21-24 part of 6-7 part, by hyaluronate sodium and go from
Sub-water mixes, and reacts 12-13h, prepare sodium hyaluronate solution at 11-14 DEG C.
The preparation method of the gel stent of a kind of repairing articular cartilage the most according to claim 1 damage, it is characterised in that
In described S2, prepare PLGA nano-particle and the azo-bis-isobutyrate hydrochloride of 5-6 part that weight portion is 7-8 part, PLGA is received
Rice grain adds in sodium hyaluronate solution, reacts 11-14min, be subsequently adding azo two 2,2-Dimethylaziridine hydrochloric acid at 16-19 DEG C
Salt, reacts 22-28min at 21-24 DEG C, prepares hyaluronic acid sodium gel complex solution.
The preparation method of the gel stent of a kind of repairing articular cartilage the most according to claim 1 damage, it is characterised in that
In described S3, hyaluronic acid sodium gel complex solution is filled to articular cartilage damage to be repaired, then through ultraviolet point light
Source is irradiated, and obtains the gel stent of repairing articular cartilage damage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610577602.2A CN106075574A (en) | 2016-07-21 | 2016-07-21 | A kind of preparation method of the gel stent of repairing articular cartilage damage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610577602.2A CN106075574A (en) | 2016-07-21 | 2016-07-21 | A kind of preparation method of the gel stent of repairing articular cartilage damage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106075574A true CN106075574A (en) | 2016-11-09 |
Family
ID=57448775
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610577602.2A Withdrawn CN106075574A (en) | 2016-07-21 | 2016-07-21 | A kind of preparation method of the gel stent of repairing articular cartilage damage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106075574A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070196342A1 (en) * | 2005-12-14 | 2007-08-23 | Sadozai Khalid K | Meniscal implant of hyaluronic acid derivatives for treatment of meniscal defects |
| US20070202084A1 (en) * | 2005-12-14 | 2007-08-30 | Anika Therapeutics, Inc. | Bioabsorbable implant of hyaluronic acid derivative for treatment of osteochondral and chondral defects |
| CN102727424A (en) * | 2012-07-13 | 2012-10-17 | 常州药物研究所有限公司 | Sodium hyaluronate gel injection for bone joint cavity and preparation method thereof |
| CN104225677A (en) * | 2013-06-13 | 2014-12-24 | 山东省生物药物研究院 | Cross-linked hyaluronic acid cell-scaffold material and preparation method and application |
| CN104587531A (en) * | 2014-12-25 | 2015-05-06 | 南京臻泉医药科技有限公司 | Preparation method for gel scaffold for repairing articular cartilage injuries |
-
2016
- 2016-07-21 CN CN201610577602.2A patent/CN106075574A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070196342A1 (en) * | 2005-12-14 | 2007-08-23 | Sadozai Khalid K | Meniscal implant of hyaluronic acid derivatives for treatment of meniscal defects |
| US20070202084A1 (en) * | 2005-12-14 | 2007-08-30 | Anika Therapeutics, Inc. | Bioabsorbable implant of hyaluronic acid derivative for treatment of osteochondral and chondral defects |
| CN102727424A (en) * | 2012-07-13 | 2012-10-17 | 常州药物研究所有限公司 | Sodium hyaluronate gel injection for bone joint cavity and preparation method thereof |
| CN104225677A (en) * | 2013-06-13 | 2014-12-24 | 山东省生物药物研究院 | Cross-linked hyaluronic acid cell-scaffold material and preparation method and application |
| CN104587531A (en) * | 2014-12-25 | 2015-05-06 | 南京臻泉医药科技有限公司 | Preparation method for gel scaffold for repairing articular cartilage injuries |
Non-Patent Citations (4)
| Title |
|---|
| 任杰等: "《氟复合材料应用技术》", 31 January 1997, 科学技术文献出版社 * |
| 张瀚文: "油田用丙烯酰胺类聚合物的研究发展", 《化工管理》 * |
| 赵亚奇等: "《高分子自由基合成技术研究》", 31 December 2017, 中央民族大学出版社 * |
| 金国珍等: "《工程塑料》", 31 January 2001, 化学工业出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mohan et al. | Chitosan-hyaluronic acid hydrogel for cartilage repair | |
| CN101810855B (en) | II-type collagen joint cartilage fluid and preparation method thereof | |
| CN102470190B (en) | Be applicable to treat the biomaterial of lacking of proper care in osteoarthritis, ligament injury and joint | |
| CN104587531B (en) | A kind of preparation method of gel stent of repairing articular cartilage damage | |
| Zhao et al. | Osteogenic media and rhBMP-2-induced differentiation of umbilical cord mesenchymal stem cells encapsulated in alginate microbeads and integrated in an injectable calcium phosphate-chitosan fibrous scaffold | |
| CN103356694B (en) | The long-acting medicament for the treatment of of arthritis disease | |
| Chen et al. | PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury | |
| CN102630157B (en) | Injectable compositions for intra-articular use combining a viscosupplementation agent and a fibroblast growth medium | |
| CN111068116B (en) | Cartilage repair temperature-sensitive gel for injection and preparation method thereof | |
| CN105435307A (en) | Natural-tissue-derived decellularized and decalcified bone material and preparation method thereof | |
| Zhao et al. | Osteogenic growth peptide accelerates bone healing during distraction osteogenesis in rabbit tibia | |
| CN103007358B (en) | Cartilage tissue engineering fiber scaffold material and preparation method thereof | |
| CN106397795A (en) | Mixed hyaluronic-acid gel and preparation method thereof | |
| Pak et al. | Potential use of mesenchymal stem cells in human meniscal repair: current insights | |
| Zhao et al. | Recent developments and current applications of hydrogels in osteoarthritis | |
| Zhang et al. | Repair of osteochondral defect using icariin-conditioned serum combined with chitosan in rabbit knees | |
| CN106668950A (en) | Fibroin three-dimensional bracket for nervus centralis remediation | |
| JP6456826B2 (en) | Ex vivo cartilage generation from fibroblasts | |
| Sun et al. | Advances in the Treatment of Partial-Thickness Cartilage Defect | |
| CN106075574A (en) | A kind of preparation method of the gel stent of repairing articular cartilage damage | |
| CN105107018B (en) | A kind of preparation method of sterile injection material | |
| EP2136783B1 (en) | A method of promoting muscle tissue repair | |
| CN106692179A (en) | Pharmaceutical preparation containing low molecular weight xanthan gum for intra-articular injection and preparation method of pharmaceutical preparation | |
| KR101637883B1 (en) | Polyethylene glycol hydrogel injection | |
| CN108042567A (en) | For the life assemblage preparation of repair of cartilage and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20161109 |