CN106074679A - A kind of Radix Puerariae granule and Chinese medicine preparation thereof - Google Patents
A kind of Radix Puerariae granule and Chinese medicine preparation thereof Download PDFInfo
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- CN106074679A CN106074679A CN201610454185.2A CN201610454185A CN106074679A CN 106074679 A CN106074679 A CN 106074679A CN 201610454185 A CN201610454185 A CN 201610454185A CN 106074679 A CN106074679 A CN 106074679A
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- 239000008187 granular material Substances 0.000 title claims abstract description 114
- 239000003814 drug Substances 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 76
- 239000000843 powder Substances 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 238000009835 boiling Methods 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000007921 spray Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 5
- 238000007908 dry granulation Methods 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 52
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 claims description 27
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 claims description 27
- 238000002329 infrared spectrum Methods 0.000 claims description 23
- 238000000605 extraction Methods 0.000 claims description 21
- 238000001228 spectrum Methods 0.000 claims description 20
- 239000000470 constituent Substances 0.000 claims description 15
- 230000003595 spectral effect Effects 0.000 claims description 15
- 238000000862 absorption spectrum Methods 0.000 claims description 10
- 238000013210 evaluation model Methods 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 10
- 238000005516 engineering process Methods 0.000 claims description 9
- 239000008188 pellet Substances 0.000 claims description 8
- 238000002835 absorbance Methods 0.000 claims description 6
- 238000003705 background correction Methods 0.000 claims description 5
- 238000009826 distribution Methods 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 3
- 238000010606 normalization Methods 0.000 claims description 3
- 239000002671 adjuvant Substances 0.000 claims description 2
- 230000008676 import Effects 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 7
- 239000000047 product Substances 0.000 description 16
- 238000002790 cross-validation Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 241000219780 Pueraria Species 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000003908 quality control method Methods 0.000 description 4
- 244000046146 Pueraria lobata Species 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000000857 drug effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 239000013558 reference substance Substances 0.000 description 3
- 238000001694 spray drying Methods 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920002774 Maltodextrin Polymers 0.000 description 2
- 239000005913 Maltodextrin Substances 0.000 description 2
- 235000010575 Pueraria lobata Nutrition 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 229940035034 maltodextrin Drugs 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 241001411320 Eriogonum inflatum Species 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000001142 anti-diarrhea Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 208000035850 clinical syndrome Diseases 0.000 description 1
- 238000010960 commercial process Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- YTJSFYQNRXLOIC-UHFFFAOYSA-N octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[SiH3] YTJSFYQNRXLOIC-UHFFFAOYSA-N 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- -1 sets moisture≤8.0% Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
- G01N2021/3572—Preparation of samples, e.g. salt matrices
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Abstract
The invention discloses a kind of Radix Puerariae granule and Chinese medicine preparation thereof.Described Radix Puerariae granule is prepared by the method comprised the steps: take Radix Puerariae decoction pieces, boiling 23 times, adds 5 10 times amount water every time, and heating decocts 12 hours；Medicinal liquid merges, and filtrate is concentrated into the clear paste that relative density is 1.02 1.10, filters with 250 350 mesh sieves while hot；Qinghuo reagent is spray-dried, and obtains spray powder；By spray powder dry granulation, obtain the granularity Radix Puerariae granule at 16 40 mesh.The Radix Puerariae granule preparing process of the present invention is simple, and technological parameter is easy to control, and has science, quick, workable detection method.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicine preparation, particularly relate to a kind of Radix Puerariae granule and Chinese medicine preparation thereof.
Background technology
Radix Puerariae derives from the dry root of legume pueraria lobata Pueraria lobata (Willd.) Ohwi, sweet, pungent, cool, returns
Spleen, stomach, lung meridian, have expelling pathogenic factors from muscles for reducing heat, promoting the production of body fluid to quench thirst, rash, yang invigorating antidiarrheal, dredge the meridian passage, deinebriating effect.
Chinese medicinal granule be the Chinese medicine decoction pieces to meet concocted specification as raw material, extract through modern industry, concentrate, dry
Dry, pelletize and the Chinese medicine series of products made, its function, cure mainly, nature and flavor, return through consistent with Chinese medicine decoction pieces, as new
Decoction pieces form replaces the prepared slices of Chinese crude drugs to control for clinical syndrome differentiation opinion, with card plus-minus, formula.With Chinese medicine decoction " decoction pieces is used as medicine,
Face and decoct with existing " application method compare, Chinese medicinal granule had both maintained the property of medicine drug effect of the former prepared slices of Chinese crude drugs, and without decocting when using
Boil, easy to carry, safe and sanitary, quality controllable, and can add and subtract with disease, meet the allegro life style of modern.
At present, Chinese medicinal granule mostly is tradition water extraction, or extracts extraction after volatile oil, and different preparation technologies leads
The product quality causing to be produced by different manufacturers is inconsistent, and drug effect exists difference.Accordingly, it would be desirable to the extraction process of research standard,
Production process with specification Chinese medicinal granule, it is ensured that drug effect.It addition, the quality of Chinese medicinal granule also can be by detection means
Restriction, the mensuration of moisture and ethanol soluble extraction in traditional Radix Puerariae granule, need several hours with reference to the method for pharmacopeia, operation
The most complicated.The main chemical compositions of Radix Puerariae granule is puerarin, and as quality control index, traditional method uses efficient liquid phase
Chromatographys etc., often need to carry out numerous and diverse pretreatment to sample, expend substantial amounts of reagent, and environmental pollution is relatively big and to coroner's body
Body has damaged, and information feedback is delayed, it is impossible to meet instant analysis multi items, many batch samples during granule enterprise large-scale production
The needs of product.
Along with the development in epoch, near infrared spectrum (NIRS) analytical technology have employed in Chemical Measurement polynary time further
Return method and contemporary optics, microcomputer data processing so that near-infrared is developed rapidly, become and dividing in recent years
The emerging analytical technology of one " green " of analysis chemical field fast development, have applied widely, measurement is convenient, pollution-free,
The advantages such as nothing is destroyed, data are accurate, reliable, are therefore widely used in food, the quantitative analysis in the various field of medicine and qualitative mirror
Not.
Summary of the invention
The problem existed for above-mentioned prior art, it is an object of the invention to provide a kind of normalized Pueraria lobota of production technology
Root granule, its preparation technology is simple, steady quality.Meanwhile, in order to better control over the quality of Radix Puerariae granule, adapt to industry metaplasia
To product batch in the demand of X-ray inspection X during product, additionally provide one fast based on near-infrared spectrum technique fast and accurately
The method of speed detection Radix Puerariae granule.
In order to solve the technical problem of the present invention, the present invention is achieved by the following technical solutions:
On the one hand, the invention provides a kind of Radix Puerariae granule, the method comprised the steps prepare: take kudzuvine root drink
Sheet, boiling 2-3 time, add 5-10 times amount water every time, heating decocts 1-2 hour；Medicinal liquid merges, and filtrate is concentrated into relative density
For the clear paste of 1.02-1.10, filter with 250-350 mesh sieve while hot；Qinghuo reagent is spray-dried, and obtains spray powder；By spray powder
Dry granulation, obtains the granularity Radix Puerariae granule at 16-40 mesh.
Present inventor is found by long term test, Radix Puerariae boiling 2-3 time, adds 5-10 times amount water every time, adds
Heat decocts and can be extracted more completely by effective ingredient for 1-2 hour.And concentrating the filtrate to relative density is 1.02-1.10's
Clear paste, filters with 250-350 mesh sieve while hot, quickly can be removed by the moisture in clear paste by the method being spray-dried, and anti-
The loss of effective ingredient is only caused because heated time is longer.
Preferably, the Radix Puerariae granule of the present invention is prepared by the method comprised the steps: takes Radix Puerariae decoction pieces, adds decocting
Boiling 3 times, add 8 times amount water every time, heating decocts 1.5 hours every time；Medicinal liquid merge, filtrate be concentrated into relative density be 1.08 clear
Cream, filters with 350 mesh sieves while hot；In inlet temperature 150-190 DEG C, expect revolution speed 400-700 rev/min, leaving air temp 80-95
DEG C, wind is spray-dried under conditions of sending temperature 35-45 DEG C, obtains spray powder；At punched-plate aperture 1.50mm, roller motor frequency
Dry granulation under the conditions of rate 40-50HZ, feeding electric machine frequency 30-50HZ, oil cylinder working-pressure 16-24bar, obtains granularity at 16-40 mesh
Radix Puerariae granule.
The process for producing optimized by the present invention, moisture≤8.0% in gained Radix Puerariae granule, ethanol soluble extraction >=
26.0%, puerarin >=52mg/g.
The Radix Puerariae granule of the present invention can further apply in various Chinese medicine preparation, to realize the pharmacologically active of Radix Puerariae.Often
The Chinese medicine preparation comprising Radix Puerariae granule seen is known in the art, those skilled in the art can be combined as required and
Application.Preferably, Chinese medicine preparation can add pharmaceutically acceptable adjuvant, such as maltodextrin.
On the other hand, in order to adapt in commercial process product batch in the demand of X-ray inspection X, overcome traditional
High effective liquid chromatography for detecting pretreatment is numerous and diverse, reagent consumption big, environmental pollution is relatively big, delayed the lacking of information feedback
Point, additionally provides a kind of detection method for Radix Puerariae granule of the present invention, and it quickly detects based on near-infrared spectrum technique, and wraps
Include following steps:
A. the collection of near infrared spectrum: take Radix Puerariae particulate samples about 3g, be ground into fine powder, carry out near infrared spectrum scanning, adopt
Collection spectrum, obtains the original absorbance spectrogram of Radix Puerariae particulate samples, and is measured each constituent content, measure Radix Puerariae granular
The measured value of each constituent content of product, determines calibration set and checking collection according to the distribution of sample number and sample each constituent content measured value；
B. the foundation of Radix Puerariae particle quantitative model: measure the content of Radix Puerariae pellet moisture, uses first derivative+MSC method pair
Original absorbance spectrum carries out pretreatment, and the spectral region of modeling chooses 5451.7-4598.8cm-1Characteristic wave bands, dimension elects 7 as；
Measure Radix Puerariae granule ethanol soluble extraction content, use first derivative+MSC method that original absorbance spectrum is carried out pretreatment, modeling
Spectral region is chosen for 6100.5-4247.9cm-1Characteristic wave bands, dimension elects 9 as；Measure Radix Puerariae granule puerarin content, use
First derivative+vector normalization method carries out pretreatment to original absorbance spectrum, and the spectral region of modeling is chosen for 6100.5-
4598.8cm-1Characteristic wave bands, dimension elects 9 as；Use partial least square method to the near infrared spectrum of calibration set and its corresponding Radix Puerariae
Quantitative model is set up between the measured value of each constituent content of particulate samples；
C. checking near-infrared quantitative model: gather checking collection sample near infrared light spectrogram, by step b set up each
Measured portions model, it is thus achieved that the predictive value of each constituent content of Radix Puerariae particulate samples, compares predictive value with measured value, checking
The accuracy of quantitative model；
D. the mensuration of each constituent content of Radix Puerariae granule: take Radix Puerariae granule to be detected and carry out the reddest according to the method for step a
External spectrum gathers, and in the quantitative model set up specific band spectral information steps for importing b, measures containing of each component of Radix Puerariae granule
Amount；By soft by near-infrared analysis for the near-infrared quantitative determination model of the Radix Puerariae pellet moisture, extractum and the puerarin that are built up
Part is integrated, and sets up the multi-method evaluation model of Radix Puerariae granule, by Radix Puerariae particulate samples to be detected according to the method collection of step a
Near infrared spectrum, imports in Radix Puerariae granule multi-method evaluation model, measures each constituent content of Radix Puerariae granule simultaneously.
Utilize above-mentioned detection method, the moisture of Radix Puerariae granule, ethanol soluble extraction and puerarin content can be measured simultaneously, it is only necessary to
A few minutes just can complete the detection of each component data, substantially reduces the detection time, and adaptation modern enterprise is quick, great Liang Sheng
The demand produced.And traditional detection method, use high effective liquid chromatography for measuring puerarin content, moisture analysis content,
Ethanol-soluble extractives algoscopy measures ethanol soluble extraction content, complex operation, not only needs multiple instrument and equipment, also needs each to examine
Testing post to cooperate and just can smoothly complete, data handling procedure is more complicated, time-consuming.Meanwhile, the method for the present invention can be simultaneously
Each constituent content of the material being suitable for 3 production links measures, including extractum, spray drying powder, granule.
In order to more accurately measure, in the assay method of the present invention, prepared by the sample that Radix Puerariae granule near-infrared measures
Method is: take same batch Radix Puerariae granule about 3g, is ground into fine powder, crosses 80 mesh sieves, transfers to the tool plug that near-infrared measures
In vial.Preferably, grinding fine powder use funnel is shifted, makes it tight by the way of up-down vibration, use rubber
Bottle stopper stoppers.It is highly preferred that under the conditions of temperature 18-22 DEG C, humidity 40-50%, granule is ground and quickly bottles.See
Examine bottom the sample bottle after dress sample, it is ensured that the sample powder of test does not bond bottom vial, just can carry out the reddest to sample
External spectrum scans.
The inventive method carries out pre-treatment for particulate samples, is ground into fine powder, crosses 80 mesh sieves, is transferred to tool plug glass
In Ping, by the way of up-down vibration, reducing the hole of sample powder, minimizing near infrared light the most irregularly runs and causes
Error；According to the method for the present invention, for the mensuration of Radix Puerariae extractum, appropriate amount of auxiliary materials can be added by technological requirement, then with micro-
Ripple stove rapid draing, dry extract can complete detection by measuring particl method, for spray drying powder, the most directly by measuring granule
Method, greatly reduces the workload that model is set up, it is achieved the quality control of key link material each to Radix Puerariae granule.
Preferably, in step a, the condition of scanning of described NIR spectra collection is: sweep limits is 4000cm-1-
12000cm-1, scanning times is 32 times, and resolution is 8cm-1, real-time background correction in scanning process, every part of sample collecting 3
Spectrum.
The inventive method has the function of detection by quantitative and qualitative identification.Quantitative determine at Radix Puerariae granule each component near-infrared
On the basis of model, establish Radix Puerariae granule multi-method evaluation model, moisture in sample, second can be drawn by the collection of a spectrum
The multicomponent content results such as alcohol extract and puerarin, can distinguish again the kind true and false according to mahalanobis distance with density of fraction etc. simultaneously
The inventive method is particularly suited for the products characteristics of Chinese medicinal granule, owing to Chinese medicinal granule is through single decoction pieces processing system
Becoming, compare with Chinese native medicine compound prescription pellet or Chinese patent medicine, component is the most single, and employing this method is disturbed less, accuracy is high；In it addition,
Medicine granule series of products have kind more than 500, and due to wide in variety, conventional method is difficult to realize qualitative, quantitative detection, the present invention simultaneously
Qualitative and quantitative analysis can be completed on the premise of easy, pollution-free simultaneously, find product abnormal risk in time, big to granule
The quality control producing intermediate link has great importance.
Therefore, the present invention compared with prior art, has the advantages that the preparation work of (1) specification Radix Puerariae granule
Skill, and preparation technology is simple, technological parameter is stable and easy to control, is suitable for the preparation of kudzu root dispensing granule.(2) present invention is based on closely
Infrared spectrum technology combines chemometrics method, in certain spectral region, establishes Radix Puerariae granule near-infrared quantitative model,
Can be used to qualitative identification, it is not necessary to sample is carried out the pre-treatment of complexity, is ground into fine powder, is not related to any examination simultaneously
Agent, environmental protection, safe and nontoxic, be the trend of Quality Control job development from now on, for detection Radix Puerariae granule provide a kind of quickly, accurately
New method.The online quality-monitoring that can promote the use in Chinese medicinal granule enterprise production process as a kind of practical approach, right
Abnormal products can be pointed out in time, it is ensured that production quality stable, feasible.
Accompanying drawing explanation
The near-infrared original absorbance spectrogram of Fig. 1 Radix Puerariae particulate samples；
Relevant figure between Fig. 2 Radix Puerariae pellet moisture near-infrared predictive value and measured value；
Fig. 3 Radix Puerariae pellet moisture near-infrared predictive value and the comparison bar diagram of measured value；
Relevant figure between Fig. 4 Radix Puerariae granule ethanol soluble extraction near-infrared predictive value and measured value；
Fig. 5 Radix Puerariae granule ethanol soluble extraction near-infrared predictive value and the comparison bar diagram of measured value；
Relevant figure between Fig. 6 Radix Puerariae granule puerarin near-infrared predictive value and measured value；
Fig. 7 Radix Puerariae granule puerarin near-infrared predictive value and the comparison bar diagram of measured value.
Detailed description of the invention
Further illustrating the present invention below by detailed description of the invention, following example are the embodiment party that the present invention is concrete
Formula, but embodiments of the present invention are not limited by following embodiment.
Embodiment 1:
The preparation of Radix Puerariae granule: take Radix Puerariae decoction pieces, boiling 3 times, add 8 times amount water every time, heating decoction 1.5 is little every time
Time；Medicinal liquid merges, and filtrate is concentrated into the clear paste that relative density is 1.08 (80 DEG C), filters with 350 mesh sieves while hot；In inlet temperature
160 DEG C, expecting revolution speed 400 revs/min, leaving air temp 90 DEG C, wind is spray-dried under conditions of sending temperature 45 C, obtains spray drying
Powder；Do under the conditions of punched-plate aperture 1.50mm, roller motor frequency 40HZ, feeding electric machine frequency 50HZ, oil cylinder working-pressure 18bar
Method is pelletized, and obtains the granularity Radix Puerariae granule at 40 mesh.
Embodiment 2:
Preparation containing the Chinese medicine preparation of Radix Puerariae granule: take Radix Puerariae decoction pieces, boiling 3 times, adds 8 times amount water, every time every time
Heating decocts 1.5 hours；Medicinal liquid merges, and filtrate is concentrated into the clear paste that relative density is 1.08 (80 DEG C), while hot with 350 mesh sieve filters
Cross；Inlet temperature 160 DEG C, expecting revolution speed 400 revs/min, leaving air temp 90 DEG C, wind is sprayed under conditions of sending temperature 45 C
It is dried, obtains spray powder；At punched-plate aperture 1.50mm, roller motor frequency 40HZ, feeding electric machine frequency 50HZ, oil cylinder working-pressure
Dry granulation under the conditions of 18bar, obtains the granularity Radix Puerariae granule at 40 mesh；170g Radix Puerariae granule is mixed with 65g maltodextrin, adds
Enter appropriate magnesium stearate, mixing, it is pressed into 1000.
Embodiment 3:
A kind of method quickly detecting Radix Puerariae granule moisture level based on near-infrared spectrum technique, specifically includes following step
Rapid:
A, take Radix Puerariae particulate samples, totally 88 batches, respectively take about 3g, be ground into fine powder, load in tool plug glass sample bottle, be placed near
It is scanned on infrared scanner, gathers spectrum, the condition of scanning: sweep limits 4000-12000cm-1, scanning times: 32 times,
Resolution 8cm-1, real-time background correction in scanning process, 3 spectrum of every part of sample collecting, obtain 88 batches of Radix Puerariaes as shown in Figure 1
The near-infrared original absorbance spectrogram of granule；
Meanwhile, according to the standard method of regulation in 2015 editions Chinese Pharmacopoeias, its moisture uses oven drying method to measure, and takes and grinds
Finely disintegrated Radix Puerariae granule about 2g, is laid in and is dried to the flat weighing botle of constant weight, accurately weighed, opens bottle cap at 100-
105 DEG C are dried 5 hours, are built by bottle cap, in dislocation exsiccator, cool down 30 minutes, accurately weighed, then are dried 1 at said temperature
Hour, cooling, weigh, to the double difference weighed less than 5mg；According to the weight of less loss, calculate Radix Puerariae granule
Water content (%), according to sample number and sample distribution determine calibration set and checking collection；
B, be distributed according to sample number and sample moisture measured value, choose 72 batches for calibration set, choose 16 batches of checking collection.
Analysis software, Automatic Optimal chooses preprocess method and spectral region, and optimum results is shown in Table 1.Use first derivative+MSC
Method carries out pretreatment to near-infrared original absorbance spectrum, chooses 5451.7-4598.3cm-1Spectral information under characteristic wave bands, fortune
The quantitative model between near infrared spectrum and moisture measured value is set up with partial least square method (PLS)；Sample by checking collection
Product carry out near infrared spectrum scanning, utilize model, obtain the predictive value of Radix Puerariae particulate samples content, are entered with measured value by predictive value
Row compares, and the accuracy of checking quantitative model and predictive ability, this model obtains cross validation coefficient R through cross validation2=
0.883, RMSECV=0.156, RPD=2.92, dimension is elected 7 as, is verified collection coefficient R2=0.933, RMSEP=0.11,
The determination of moisture of 16 batches of Radix Puerariae granules the results are shown in Table 2, and the relevant figure between moisture predictive value and measured value is shown in Fig. 2, moisture
Fig. 3 is shown in the comparison bar diagram of predictive value and measured value.
Table 1 water model is in different spectral regions and the model parameter under preprocess method
The determination of moisture result of 2 16 batches of Radix Puerariae granules of table
By upper table 2 it can be seen that the deviation range of Radix Puerariae granule moisture level is between 0.00-0.22%, consensus forecast
The response rate is 100.08%, illustrates that this model has preferable predictive ability and stability, can be used for the fast of Radix Puerariae pellet moisture
Speed detection.
Embodiment 4:
A kind of method quickly detecting Radix Puerariae granule ethanol soluble extraction content based on near-infrared spectrum technique, specifically include as
Lower step:
A, take Radix Puerariae particulate samples, totally 88 batches, respectively take about 3g, be ground into fine powder, load in tool plug glass sample bottle, be placed near
It is scanned on infrared scanner, gathers spectrum, the condition of scanning: sweep limits 4000-12000cm-1, scanning times: 32 times,
Resolution 8cm-1, real-time background correction in scanning process, 3 spectrum of every part of sample collecting, obtain 88 batches of Radix Puerariaes as shown in Figure 1
The near-infrared original absorbance spectrogram of granule；
Meanwhile, according to the standard method of regulation in 2015 editions Chinese Pharmacopoeias, its ethanol soluble extraction content uses hot dipping to survey
Fixed, take the Radix Puerariae granule about 2g grinding to form fine powder, accurately weighed, to put in the conical flask of 100ml, precision adds ethanol 100ml, close
Plug, weighed weight, after standing 1 hour, connect reflux condensing tube, be heated to boiling, and keep micro-boiling 1 hour；After letting cool, take off
Conical flask, close plug, more weighed weight, supply the weight of less loss, shake up with ethanol, filters with being dried filter, and precision measures filtrate
50ml, puts after being dried to the evaporating dish of constant weight, being evaporated in water-bath, is dried 3 hours in 105 DEG C, put in exsiccator and cool down
30 minutes, the close weighed weight of rapid nationality；Unless otherwise specified, the content of ethanol-soluble extractives in Radix Puerariae granule is calculated with dry product
(%), calibration set and checking collection are determined according to sample number and sample distribution；
B, be distributed according to sample number and sample ethanol soluble extraction content measured value, choose 72 batches for calibration set, choose 16 batches
For checking collection.Analysis software, Automatic Optimal chooses preprocess method and spectral region, and optimum results is shown in Table 3.Use single order
Derivative+MSC method carries out pretreatment to near-infrared original absorbance spectrum, chooses 6100.5-4247.9cm-1Spectrum under characteristic wave bands
Information, uses partial least square method (PLS) to set up the quantitative model between near infrared spectrum and ethanol soluble extraction content measured value；
The sample of checking collection is carried out near infrared spectrum scanning, utilizes model, obtain the predictive value of Radix Puerariae particulate samples content, will prediction
Value compares with measured value, and the accuracy of checking quantitative model and predictive ability, this model obtains cross validation through cross validation
Coefficient R2=0.864, RMSECV=1.68, RPD=2.71, dimension is elected 9 as, is verified collection coefficient R2=0.849,
RMSEP=1.56, the ethanol soluble extraction assay of 16 batches of Radix Puerariae granules the results are shown in Table 4, ethanol soluble extraction predictive value and actual measurement
Relevant figure between value is shown in that Fig. 5 is shown in the comparison bar diagram of Fig. 4, ethanol soluble extraction predictive value and measured value.
Table 3 ethanol soluble extraction model is in different spectral regions and the model parameter under preprocess method
The ethanol soluble extraction assay result of 4 16 batches of Radix Puerariae granules of table
By upper table 4 it can be seen that the deviation range of Radix Puerariae granule ethanol soluble extraction content is between 0.16-3.58%, in advance
Surveying average recovery rate is 100.30%, illustrates that this model has preferable predictive ability and stability, can be used for Radix Puerariae granule second
The quick detection of alcohol extract.
Embodiment 5:
A kind of quickly detect the method for puerarin content in Radix Puerariae granule based on near-infrared spectrum technique, specifically include as follows
Step:
A, take Radix Puerariae particulate samples, totally 88 batches, respectively take about 3g, be ground into fine powder, load in tool plug glass sample bottle, be placed near
It is scanned on infrared scanner, gathers spectrum, the condition of scanning: sweep limits 4000-12000cm-1, scanning times: 32 times,
Resolution 8cm-1, real-time background correction in scanning process, 3 spectrum of every part of sample collecting, obtain 88 batches of Radix Puerariaes as shown in Figure 1
The near-infrared original absorbance spectrogram of granule；
Meanwhile, according to the standard method of regulation in 2015 editions Chinese Pharmacopoeias, its puerarin content is according to high performance liquid chromatography
(four general rules 0512 of " Chinese Pharmacopoeia " version in 2015) measure, and specifically comprise the following steps that
Chromatographic condition and system suitability: with octadecylsilane chemically bonded silica as filler；With methanol-water (25:
75) for flowing phase；Detection wavelength is 250nm.Number of theoretical plate is calculated by puerarin peak should be not less than 2000.
The preparation of reference substance solution: take puerarin reference substance appropriate, accurately weighed, add 30% ethanol and make every 1ml containing 80 μ
The solution of g, to obtain final product.
The preparation of need testing solution: take this product under content uniformity item, finely ground, take about 0.1g, accurately weighed, put tool plug cone
In shape bottle, accurate addition 30% ethanol 100ml, weighed weight, supersound process (power 300W, frequency 40kHz) 30 minutes, put
Cold, more weighed weight, supply the weight of less loss with 30% ethanol, shake up, filter, take subsequent filtrate, to obtain final product.
Algoscopy: precision draws reference substance solution and each 10 μ l of need testing solution respectively, injects chromatograph of liquid, measures,
Obtain.
B, be distributed according to sample number and sample puerarin content measured value, choose 72 batches for calibration set, choose 16 batches for testing
Card collection.Analysis software, Automatic Optimal chooses preprocess method and spectral region, and optimum results is shown in Table 5.Use first derivative
+ vector normalization method carries out pretreatment to near-infrared original absorbance spectrum, chooses 6100.5-4598.8cm-1Under characteristic wave bands
Spectral information, uses partial least square method (PLS) to set up the quantitative model between near infrared spectrum and puerarin standard content；Will
The sample of checking collection carries out near infrared spectrum scanning, utilizes model, obtains the predictive value of Radix Puerariae particulate samples content, by predictive value
Comparing with measured value, the accuracy of checking quantitative model and predictive ability, this model obtains cross validation phase through cross validation
Close coefficients R2=0.912, RMSECV=3.31, RPD=3.37, dimension is elected 9 as, is verified collection coefficient R2=0.900, RMSEP
The puerarin content measurement result of=3.74,16 batches of Radix Puerariae granules is shown in Table 6, the relevant figure between puerarin predictive value and measured value
See that Fig. 7 is shown in the comparison bar diagram of Fig. 6, puerarin predictive value and measured value.
Table 5 puerarin model is in different spectral regions and the model parameter under preprocess method
6 16 batches of Radix Puerariae granule puerarin content measurement results of table
By upper table 6 it can be seen that the deviation range of Radix Puerariae granule puerarin is between 0.00-7.21mg/g, it was predicted that average
The response rate is 100.55%.Illustrate that this model has preferable predictive ability and stability, can be used for Radix Puerariae granule puerarin
Quickly detection.
Embodiment 6:
A kind of method based on near-infrared spectrum technique Fast Evaluation Radix Puerariae granular mass, specifically includes following steps:
A. use software, the Radix Puerariae granule puerarin built, moisture and extractum quantitative model are imported, and sets each group
The content limit divided, according to the quality standard of Radix Puerariae granule, sets moisture≤8.0%, ethanol soluble extraction >=26.0%, puerarin
>=52mg/g, sets up the multi-method evaluation model of Radix Puerariae granule；
B. gather sample near-infrared absorption spectrum figure, spectrogram is imported in multi-method evaluation model, read each group of sample
The content divided and evaluation information.
For verifying predictive ability and the accuracy of this model, the present invention uses mono blind method to test, to Radix Puerariae, Radix Salviae Miltiorrhizae etc. 10 batches
Particulate samples is evaluated, and sample message table is shown in Table 7.Gather the spectrogram of numbering 1～10 sample respectively, spectrogram is imported Pueraria lobota
In root granule multi-method evaluation model, read content and the evaluation information of each component of sample, the results are shown in Table 8.
Table 7 multi-method evaluation model verification sample information table
Table 8 Radix Puerariae granule multi-method evaluation result
-representing surveyed sample passes, * represents institute's test sample product abnormal (different samples or content are undesirable)
As can be seen from Table 8, this multi-method evaluation model can according to sample spectra information, analyze draw mahalanobis distance,
Whether density of fraction, by the size of its value, can be Radix Puerariae granule with principium identification institute test sample product, be identified as Radix Puerariae granule
Sample can draw the accurate predictor of each component simultaneously.Illustrate that this model has preferable predictive ability and accuracy, can be quick
Evaluate the quality of Radix Puerariae granule.
Claims (10)
1. a Radix Puerariae granule, it is characterised in that prepared by the method comprised the steps: take Radix Puerariae decoction pieces, boiling
2-3 time, adding 5-10 times amount water, heating decocts 1-2 hour every time；Medicinal liquid merges, and it is 1.02-1.10 that filtrate is concentrated into relative density
Clear paste, while hot with 250-350 mesh sieve filter；Qinghuo reagent is spray-dried, and obtains spray powder；By spray powder dry granulation,
Granularity is at the Radix Puerariae granule of 16-40 mesh.
2. Radix Puerariae granule as claimed in claim 1, it is characterised in that prepared by the method comprised the steps: take Radix Puerariae
Decoction pieces, boiling 3 times, add 8 times amount water every time, heating decocts 1.5 hours every time；Medicinal liquid merges, and filtrate is concentrated into the closeest
Degree is the clear paste of 1.08, filters with 350 mesh sieves while hot；In inlet temperature 150-190 DEG C, expect revolution speed 400-700 rev/min, go out
Air temperature 80-95 DEG C, wind is spray-dried under conditions of sending temperature 35-45 DEG C, obtains spray powder；In punched-plate aperture
1.50mm, dry method system under the conditions of roller motor frequency 40-50HZ, feeding electric machine frequency 30-50HZ, oil cylinder working-pressure 16-24bar
Grain, obtains the granularity Radix Puerariae granule at 16-40 mesh.
3. Radix Puerariae granule as claimed in claim 1 or 2, it is characterised in that moisture≤8.0% in Radix Puerariae granule, ethanol leaches
Thing >=26.0%, puerarin >=52mg/g.
4. a Chinese medicine preparation, it is characterised in that comprise the Radix Puerariae granule as described in one of claim 1-3 and pharmaceutically can connect
The adjuvant being subject to.
5. the detection method of the Radix Puerariae granule as described in one of claim 1-3, it is characterised in that based near infrared spectrum
Technology quickly detects, and comprises the steps:
A. the collection of near infrared spectrum: take Radix Puerariae particulate samples about 3g, be ground into fine powder, carries out near infrared spectrum scanning, gathers light
Spectrum, obtains the original absorbance spectrogram of Radix Puerariae particulate samples, and is measured each constituent content, measure Radix Puerariae particulate samples each
The measured value of constituent content, determines calibration set and checking collection according to the distribution of sample number and sample each constituent content measured value；
B. the foundation of Radix Puerariae particle quantitative model: measure the content of Radix Puerariae pellet moisture, uses first derivative+MSC method to original
Absorption spectrum carries out pretreatment, and the spectral region of modeling chooses 5451.7-4598.8cm-1Characteristic wave bands, dimension elects 7 as；Measure
Radix Puerariae granule ethanol soluble extraction content, uses first derivative+MSC method that original absorbance spectrum is carried out pretreatment, the spectrum of modeling
Scope is chosen for 6100.5-4247.9cm-1Characteristic wave bands, dimension elects 9 as；Measure Radix Puerariae granule puerarin content, use single order
Derivative+vector normalization method carries out pretreatment to original absorbance spectrum, and the spectral region of modeling is chosen for 6100.5-
4598.8cm-1Characteristic wave bands, dimension elects 9 as；Use partial least square method to the near infrared spectrum of calibration set and its corresponding Radix Puerariae
Quantitative model is set up between the measured value of each constituent content of particulate samples；
C. checking near-infrared quantitative model: gather the near infrared light spectrogram of checking collection sample, each component set up by step b
Quantitative model, it is thus achieved that the predictive value of each constituent content of Radix Puerariae particulate samples, compares predictive value with measured value, and checking is quantitatively
The accuracy of model；
D. the mensuration of each constituent content of Radix Puerariae granule: take Radix Puerariae granule to be detected and carry out near infrared light according to the method for step a
Spectrum gathers, and in the quantitative model set up specific band spectral information steps for importing b, measures the content of each component of Radix Puerariae granule；
By whole by near-infrared analysis software for the near-infrared quantitative determination model of the Radix Puerariae pellet moisture, extractum and the puerarin that are built up
Close, set up the multi-method evaluation model of Radix Puerariae granule, Radix Puerariae particulate samples to be detected is gathered the reddest according to the method for step a
External spectrum, imports in Radix Puerariae granule multi-method evaluation model, measures each constituent content of Radix Puerariae granule simultaneously.
6. the detection method of Radix Puerariae granule as claimed in claim 5, it is characterised in that the sample that Radix Puerariae granule near-infrared measures
Preparation method is: take same batch Radix Puerariae granule about 3g, is ground into fine powder, crosses 80 mesh sieves, transfers to what near-infrared measured
In tool plug vial.
7. the detection method of Radix Puerariae granule as claimed in claim 6, it is characterised in that: carry out turning by grinding fine powder use funnel
Move, make it tight by the way of up-down vibration, stopper with bottle closure of rubber.
8. the detection method of Radix Puerariae granule as claimed in claim 6, it is characterised in that: in temperature 18-22 DEG C, humidity 40-
Under the conditions of 50%, granule is ground and quickly bottles.
9. the detection method of Radix Puerariae granule as claimed in claim 6, it is characterised in that: observe bottom the sample bottle after dress sample,
The sample powder of guarantee test does not bond bottom vial, just sample can be carried out near infrared spectrum scanning.
10. the detection method of Radix Puerariae granule as claimed in claim 5, it is characterised in that: in step a, described NIR spectra is adopted
The condition of scanning integrated is: sweep limits is as 4000cm-1-12000cm-1, scanning times is 32 times, and resolution is 8cm-1, scanned
Real-time background correction in journey, 3 spectrum of every part of sample collecting.
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| CN108051396A (en) * | 2017-12-14 | 2018-05-18 | 山东沃华医药科技股份有限公司 | A kind of rapid detection method of Xin Ke Shu ' tablet for treating coronary heart disease active constituent content |
| CN110970115A (en) * | 2019-10-28 | 2020-04-07 | 广西科技大学 | Informatization representation method for nature, taste and meridian tropism of traditional Chinese medicine prescription |
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Effective date of registration: 20201203 Address after: No.38, Yong'an West Road, Xinwo street, Pan'an County, Jinhua City, Zhejiang Province Patentee after: Zhejiang Yifang Pharmaceutical Co., Ltd Address before: 528244 Guangdong Province Nanhai District of Foshan City Industrial Development Zone Guangdong Qifeng town Party Pharmaceutical Co. Ltd. Patentee before: GUANGDONG YIFANG PHARMACEUTICAL Co.,Ltd. |