CN106071516A - The preparation method of Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage - Google Patents

The preparation method of Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage Download PDF

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Publication number
CN106071516A
CN106071516A CN201610336565.6A CN201610336565A CN106071516A CN 106071516 A CN106071516 A CN 106071516A CN 201610336565 A CN201610336565 A CN 201610336565A CN 106071516 A CN106071516 A CN 106071516A
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rhizoma dioscoreae
juice
pleurotus eryngii
fragariae ananssae
fructus fragariae
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CN106071516B (en
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马艳弘
黄开红
殷剑美
魏建明
李亚辉
张宏志
张立群
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Jiangsu Boda Biological Technology Co ltd
Jiangsu Academy of Agricultural Sciences
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Jiangsu Boda Biological Technology Co ltd
Jiangsu Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum

Abstract

The present invention relates to the preparation method of a kind of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage, comprise the steps: that (1) prepares Rhizoma Dioscoreae pickle juice;(2) Fructus Fragariae Ananssae slurry is prepared;(3) pleurotus eryngii juice is prepared;(4) preparation of composite juice and enzymolysis;(5) centrifugal;(6) allotment: adding Mel, xylitol, vitamin C, complex stabilizer in clarified compound juice, stirring is to being completely dissolved;(7) homogenizing: the liquid high pressure homogenizer after allotment is carried out homogenizing;(8) fill, sterilization.Obtain Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage.Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage prepared by the method is nutritious, rich in active polysaccharide, dietary fiber, strawberry polyphenol, local flavor aminoacid, both Rhizoma Dioscoreae, Pleurotus eryngii, Fructus Fragariae Ananssae original trophic function characteristic had been maintained, add again inulin and the biological activity of lactic acid bacteria metabolism generation, the most also improve Rhizoma Dioscoreae Pickles processing byproduct " pickled vegetable liquid " and the value of Pleurotus eryngii leftover pieces.

Description

The preparation method of Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage
Technical field
The present invention relates to a kind of method for preparing beverage, be specifically related to the preparation side of a kind of Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage Method, belongs to technical field of food biotechnology.
Background technology
Rhizoma Dioscoreae is the dual-purpose food of integration of edible and medicinal herbs that China is traditional, not only rich in starch, protein, aminoacid, fatty acid with Multiple mineral element, possibly together with the multiple bioactive ingredients such as active polysaccharide, polyphenol, flavone, Saponin, allantoin, allantoic acid, tool There are the multiple medicine healthy sofa functions such as antioxidation, defying age, antibacterial, anticancer, the liver protecting, enhancing human body immunity power.Steep Rhizoma Dioscoreae In the production process of dish, Chinese yam polysaccharide, albumen, Rhizoma Dioscoreae glycosides isoreactivity composition are constantly dissolved in brewed liquid, and by micro-life The metabolic process of thing, particularly lactic acid bacteria can also produce multiple flavor substance and functional factor, so that brewed liquid has possessed solely Special trophic function characteristic.But China's pickled vegetable liquid processes as waste liquid mostly at present, not only easily causes environmental pollution, also Cause the significant wastage of resource.Pleurotus eryngii is nutritious, has the effects such as hypertension and hyperlipemia, and alimentary health-care function is high.But During Fresh Apricot abalone mushroom produces, stem waste cut materials is up to about the 14% of commodity amount, and substantial amounts of leftover pieces are only used as feedstuff or fertilizer Material, resource utilization is extremely low.And Fructus Fragariae Ananssae is known as " fruit queen ", its fruit is red tender beautiful, soft and succulency, and fragrance is strong, sweet Acid is tasty and refreshing.There is not yet in the market with pickle juice as primary raw material, the fermented beverage that composite eryngii and Fructus Fragariae Ananssae are made.And show The problems such as have Yam beverage, that Pleurotus eryngii beverage all exists taste is single, health care difference, the Rhizoma Dioscoreae Fructus Fragariae Ananssae Fructus Pruni Bao of this method exploitation Mushroom composite beverage is all the improvement to existing product Yu technology at aspects such as mouthfeel, local flavor, Nutrition and health functions.
Summary of the invention
The present invention is just for technical problem present in prior art, it is provided that a kind of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor The preparation method of beverage, the method preparation is a kind of nutritious, rich in active polysaccharide, dietary fiber, strawberry polyphenol, local flavor amino The Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage of acid, to promote Rhizoma Dioscoreae pickled vegetable liquid and the value of Pleurotus eryngii leftover pieces.
To achieve these goals, technical scheme is as follows.
The preparation method of a kind of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage, described method comprises the steps:
(1) preparing Rhizoma Dioscoreae pickle juice: Rhizoma Dioscoreae is cleaned, cutting, segment length 2 ~ 4cm puts into the Pickles crossed in advance with 75% alcohol disinfecting Altar, adds the brewed liquid accounting for Rhizoma Dioscoreae quality 1 ~ 1.5 times, inoculating lactic acid bacterium leavening agent, builds altar lid, and pickle jar mouth adds water-stop, Being placed in 10 ~ 25 DEG C of environment fermentation certain time, take out Rhizoma Dioscoreae Pickles and process separately after fermentation ends, residue juice such as adds at the body Long-pending tap water dilution, obtains Rhizoma Dioscoreae pickle juice;
(2) prepare Fructus Fragariae Ananssae slurry: cleaned up by fresh strawberry, use the making beating of single track beater, obtain Fructus Fragariae Ananssae slurry, standby;
(3) pleurotus eryngii juice is prepared: after being cleaned up by Pleurotus eryngii leftover pieces, section, sheet thickness 1 ~ 2cm, puts into the boiling water of 2 times of quality Middle blanching, squeezes extracting juice by this mushroom aqueous mixtures pressafiner after being cooled to 30 ~ 40 DEG C, obtains pleurotus eryngii juice;
(4) preparation of composite juice, enzymolysis: Rhizoma Dioscoreae pickle juice, Fructus Fragariae Ananssae slurry, pleurotus eryngii juice are proportionally added into enzymatic vessel, and mixing is all Even, obtain composite juice, composite juice adds compound enzymic preparation and carries out enzymolysis;
(5) centrifugal: composite juice enzymolysis filters 15 ~ 20min with tube centrifuge after terminating, and removes filtering residue, obtains clarified compound juice;
(6) allotment: adding Mel, xylitol, vitamin C, complex stabilizer in clarified compound juice, stirring is to being completely dissolved;
(7) homogenizing: the liquid after allotment is put into and carries out homogenizing with high pressure homogenizer after chuck heating tank is heated to 65 ~ 75 DEG C, Homogenization pressure is 20 ~ 22MPa, and homogenization cycles is 2 times;
(8) fill, sterilization: direct fill, sterilizing after homogenizing, cool down, case, obtain Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor drink Material.
The present solution provides one and integrate fermentation perfume, mushroom perfume, Fructus Fragariae Ananssae perfume (or spice), fragrant, sour and sweet palatability, richness Novel fermentation drink containing beneficial functions factors such as Chinese yam polysaccharide, mushroom polysaccharide and local flavor aminoacid, dietary fiber, strawberry polyphenol Material, both the exploitation for a large amount of pickled vegetable liquid of China and Pleurotus eryngii leftover pieces opened new approach, can enrich again Rhizoma Dioscoreae, Fructus Pruni Bao Mushroom, Fructus Fragariae Ananssae deep processed product, have huge market development and be worth.
As a modification of the present invention, in described step (1), described brewed liquid is by a certain amount of Sal, white sugar, chrysanthemum Powder, Rhizoma Zingiberis add tap water mixing, 90 ~ 95 DEG C of insulation 30min, are cooled to 30 ~ 35 DEG C and make, wherein Sal, white sugar, chrysanthemum Powder, Rhizoma Zingiberis are respectively 50g/L, 30 g/L, 20 ~ 50 g/L, 10 ~ 20g/L with the mass volume ratio of water.
As a modification of the present invention, in described step (1), the inoculum concentration of leaven is the 2% ~ 5% of Rhizoma Dioscoreae quality.Connect Amount of planting is very few, and fermentation can not start in time, easily causes living contaminants;Inoculum concentration is excessive, can increase production cost.
As a modification of the present invention, in described step (1), fermentation time is 11 ~ 14d.In this step, prepare Rhizoma Dioscoreae During pickle juice, be respectively provided with 1,3,5,8,11,14,17d be fermentation time, by pH in tracing detection sweat, total acid, The Changing Pattern of the indexs such as lactic acid bacteria number, content of nitrite, coliform count, result shows to ferment 11 ~ 17d, Rhizoma Dioscoreae Pickles The lactic acid bacteria number of liquid is the highest, content of nitrite and coliform number drop to the lowest, meets China to nitrous in catsup and pickled vegetables Hydrochlorate and colibacillary limitation requirement, reached safe edible standard.Production cost can be increased in view of fermentation time is long, Therefore determine that fermentation time is 11 ~ 14d, preferably 11d.Each index is as shown in table 1 in the measurement result of different fermentations time.
As a modification of the present invention, in step (2), described strawberry cultivars is the precocious grass of Jiangsu Province Agriculture Science Institute selection-breeding Certain kind of berries kind " Ning Yu ", kind fragrance is dense, local flavor is good for this, best in quality, effectively masks the fermented flavour of pickle juice, imparts product Strong fragrance.
As a modification of the present invention, in described step (4), Rhizoma Dioscoreae pickle juice, Fructus Fragariae Ananssae slurry, the volume ratio of pleurotus eryngii juice For 2:1:1 ~ 2.
As a modification of the present invention, in described step (4), described compound enzymic preparation is by cellulase and pectase group Become, the enzyme activity >=400u/mg of its cellulase, the enzyme activity >=500u/mg of pectase.Cellulase and the matter of pectase Amount ratio is 2:1 ~ 1.5;In composite juice, the addition of compound enzymic preparation is 0.01% ~ 0.015%;Enzymatic hydrolysis condition is: temperature 60 ~ 65 DEG C, enzymolysis time 2 ~ 2.5h.
As a modification of the present invention, in described step (6), in clarified compound juice, the addition of Mel is 20 ~ 40g/ L, the addition of xylitol are 60 ~ 100g/L, vitamin C addition is 3.5 ~ 6g/L, the addition of complex stabilizer be 0.6 ~ 1.2g/L。
As a modification of the present invention, in step (6), described complex stabilizer is by the component group of following mass percent Become: pectin 18% ~ 25%, xanthan gum 26% ~ 32%, sodium carboxymethyl cellulose 45% ~ 50%.
As a modification of the present invention, in step (8), sterilising conditions is: 85 ~ 90 DEG C of sterilizing 20 ~ 25min.
As a modification of the present invention, in step (1), described lactic acid bacteria is Lactobacillus plantarum JP-8, this bacterial strain preservation In China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation center, culture presevation number is CGMCC No.8289, preservation date JIUYUE 29 in 2013.
Relative to prior art, advantages of the present invention is as follows: 1) this technical scheme uses Rhizoma Dioscoreae processing byproduct Rhizoma Dioscoreae bubble Dish liquid is a kind of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage of primary raw material preparation, is effectively improved the value of pickled vegetable liquid, Improve the comprehensive utilization ratio of resource, it is to avoid the environmental pollution that is likely to result in;2) under the present invention uses the cutting of Pleurotus eryngii stem Waste as another primary raw material, it is rich in materials such as mushroom polysaccharide, dietary fiber, local flavor aminoacid, not only makes this The functional of invention product is remarkably reinforced, and its local flavor aminoacid and Semen Armeniacae Amarum fragrance the most effectively mask the special gas of pickled vegetable liquid Taste, makes the mouthfeel of product, local flavor are all obviously improved;3) present invention also uses by the precocious Fructus Fragariae Ananssae of academy of agricultural sciences of Jiangsu Province selection-breeding Kind " Ning Yu " is one of raw material, and strawberry cultivars fragrance is dense, mouthfeel is good for this so that product is in the case of without essence and flavoring agent I.e. possesses strong fragrance;4), when prepared by Rhizoma Dioscoreae pickle juice, interpolation prebiotics inulin is used to combine purebred lactic acid bacteria fermentation skill Art achieves the quick startup of fermentation, is effectively shortened fermentation time, the living contaminants avoiding in sweat, it is ensured that produce The edible safety of product;5) have employed complex enzyme zymohydrolysis technique, improve the biological activity such as polysaccharide, polyphenol in Fructus Fragariae Ananssae slurry and Pleurotus eryngii The dissolution rate of material, improves the alimentary health-care function of product;6) use sodium carboxymethyl cellulose, pectin, xanthan gum by closing The reason complex stabilizer that is mixed of proportioning, it is to avoid the phenomenons such as the precipitation that is likely to occur in product storage, muddiness, effectively Ensure that the commodity stabilization of product;7) this technical scheme employs Mel, xylitol is sweeting agent, both can pre-anti-caries, again Give the joyful sweet taste of product without making blood glucose raise, be especially suitable for diabetes patient and hyperglycemic patients is edible;8) present invention The Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage of preparation is fine at Chinese yam polysaccharide, Polysaccharide in Pleurotus eryngii and local flavor aminoacid, inulin meals Under the synergism of the functional factors such as dimension, strawberry polyphenol, its function been significantly enhanced, the sweet and tasty mouth of product acid, fragrant, wind Taste is unique, production technology is simple, be suitable to large-scale production, easily marketing.
Detailed description of the invention
In order to deepen the understanding of the present invention and understanding, below in conjunction with detailed description of the invention, the invention will be further described And introduction.
Embodiment 1: the preparation method of a kind of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage, step is as follows,
(1) prepared by Rhizoma Dioscoreae pickle juice: fresh Chinese yam (bio tech ltd of BDCom, Jiangsu) 100kg cleans up, drains the water Point, it is cut into 2 ~ 4cm segment, puts into the pickle jar crossed with 75% alcohol disinfecting in advance, add brewed liquid, add Lactobacillus plantarum JP-8 leaven 4kg(inoculum concentration is the 4% of Rhizoma Dioscoreae quality), build altar lid, pickle jar mouth adds water-stop, is placed in 15 DEG C of environment After fermentation 11d, Pickles are taken out processing and packing separately, altar remains pickled vegetable liquid adds the dilution of 100L tap water, standby;
Described brewed liquid is added 100L certainly by a certain amount of Sal, white sugar, inulin (Fengning safety high-tech Industrial Co., Ltd.), Rhizoma Zingiberis Water mixes, 90 DEG C of insulation 30min, is cooled to 30 DEG C and makes, wherein the mass body of Sal, white sugar, inulin, Rhizoma Zingiberis and water Long-pending ratio respectively 50g/L, 30 g/L, 20 g/L, 10g/L;
(2) Fructus Fragariae Ananssae slurry preparation: fresh for 100kg peaceful beautiful Fructus Fragariae Ananssae (plant science base, Lishui, Jiangsu Province Agriculture Science Institute) is cleaned dry Only, using single track beater making beating (Shanghai Jing Sen light industry and machinery company limited), gained Fructus Fragariae Ananssae slurry is standby;
(3) prepared by pleurotus eryngii juice: after Pleurotus eryngii leftover pieces (bio tech ltd of BDCom, Jiangsu) 50kg cleans up, cut Sheet, adds blanching 5min in the boiling water of 100kg, after being cooled to 30 DEG C by this mushroom aqueous mixtures spiral juice extractor (in Xinxiang City Light Machinery Co., Ltd.) squeezing extracting juice, standby;
(4) preparation of composite juice and enzymolysis: Rhizoma Dioscoreae pickle juice 200kg, Fructus Fragariae Ananssae slurry 100kg, pleurotus eryngii juice 100kg are put into enzymolysis Tank, mix homogeneously, add the cellulase (Rui Yong bio tech ltd, Shanghai, 400u/mg) by 2:1 and pectase The compound enzymic preparation 40g(0.01% that (Rui Yong bio tech ltd, Shanghai, 400u/mg) forms), 60 DEG C of insulation enzymolysis 2h;
(5) centrifugal: the tube centrifuge (sky, PVG this centrifugal machine company limited) of the composite juice after enzymolysis is centrifuged 15min, gained clarified compound juice adds in material-compound tank standby;
(6) allotment: add Mel, xylitol, vitamin C, complex stabilizer, stirring and dissolving in material-compound tank.Clarified compound juice Addition 20g/L of middle Mel, addition 100g/L of xylitol, ascorbic addition is 3.5g/L, and complex stabilizer adds Entering amount is 0.3g/L.Described complex stabilizer is made up of the component of following mass percent: (Zhengzhou height grinds biotechnology to be had pectin Limit company) 20%, xanthan gum (Zibo Deosen Biochemical Ltd.) 30%, sodium carboxymethyl cellulose (Shen, Shanghai light edible chemical Company limited) 50%.
(7) homogenizing: pump into after chuck heating tank is heated to 65 DEG C after step (4) is deployed, through high pressure homogenizer (Sheng Tongji Tool company limited) process 2 times, homogenization pressure is 20MPa.
(8) fill, sterilization: direct fill, 85 DEG C of sterilizing 25min after step (5) homogenizing, cool down rapidly, get product.
Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage prepared by the present embodiment, possesses the fragrant strong perfume (or spice) with Pleurotus eryngii of lactic acid bacteria fermentation Gas, flavour are coordinated soft, sour and sweet palatability, and its total acid content 3.25g/L(is in terms of lactic acid), soluble solid (20 DEG C of refractometers Method) 15.2%, salinity 0.60%.
Embodiment 2:
A kind of preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage, step is as follows,
(1) prepared by Rhizoma Dioscoreae pickle juice: fresh Chinese yam (bio tech ltd of BDCom, Jiangsu) 100kg cleans up, drains the water Point, it is cut into 2 ~ 4cm segment, puts into the pickle jar crossed with 75% alcohol disinfecting in advance, add brewed liquid, add Lactobacillus plantarum JP-8 leaven 4kg(inoculum concentration is the 4% of Rhizoma Dioscoreae quality), build altar lid, pickle jar mouth adds water-stop, is placed in 15 DEG C of environment After fermentation 11d, Pickles are taken out processing and packing separately, altar remains pickled vegetable liquid adds the dilution of 100L tap water, standby;
Described brewed liquid is added 100L certainly by a certain amount of Sal, white sugar, inulin (Fengning safety high-tech Industrial Co., Ltd.), Rhizoma Zingiberis Water mixes, 90 DEG C of insulation 30min, is cooled to 30 DEG C and makes, wherein the mass body of Sal, white sugar, inulin, Rhizoma Zingiberis and water Long-pending ratio respectively 50g/L, 30 g/L, 20 g/L, 10g/L;
(2) Fructus Fragariae Ananssae slurry preparation: fresh for 100kg peaceful beautiful Fructus Fragariae Ananssae (plant science base, Lishui, Jiangsu Province Agriculture Science Institute) is cleaned dry Only, using single track beater making beating (Shanghai Jing Sen light industry and machinery company limited), gained Fructus Fragariae Ananssae slurry is standby;
(3) prepared by pleurotus eryngii juice: after Pleurotus eryngii leftover pieces (bio tech ltd of BDCom, Jiangsu) 100kg cleans up, cut Sheet, adds blanching 5min in the boiling water of 200kg, after being cooled to 30 DEG C by this mushroom aqueous mixtures spiral juice extractor (in Xinxiang City Light Machinery Co., Ltd.) squeezing extracting juice, standby;
(4) preparation of composite juice and enzymolysis: Rhizoma Dioscoreae pickle juice 200kg, Fructus Fragariae Ananssae slurry 100kg, pleurotus eryngii juice 200kg are put into enzymolysis Tank, mix homogeneously, add by adding by the cellulase (Rui Yong bio tech ltd, Shanghai, 400u/mg) of 2:1.5 The compound enzymic preparation 60g(0.012% formed with pectase (Rui Yong bio tech ltd, Shanghai, 500u/mg)), 60 DEG C of guarantors Temperature enzymolysis 2.5h;
(5) centrifugal: the tube centrifuge (sky, PVG this centrifugal machine company limited) of the composite juice after enzymolysis is centrifuged 15min, gained clarified compound juice adds in material-compound tank standby;
(6) allotment: add Mel, xylitol, vitamin C, complex stabilizer, stirring and dissolving in material-compound tank.Clarified compound juice Addition 40g/L of middle Mel, addition 80g/L of xylitol, ascorbic addition is 5g/L, and complex stabilizer adds Amount is 0.5g/L.Described complex stabilizer is made up of the component of following mass percent: (it is limited that Zhengzhou height grinds biotechnology to pectin Company) 25%, xanthan gum (Zibo Deosen Biochemical Ltd.) 30%, (Shen, Shanghai light edible chemical has sodium carboxymethyl cellulose Limit company) 45%.
(7) homogenizing: pump into after chuck heating tank is heated to 65 DEG C after step (4) is deployed, through high pressure homogenizer (Sheng Tongji Tool company limited) process 2 times, homogenization pressure is 20MPa.
(8) fill, sterilization: direct fill, 90 DEG C of sterilizing 20min after step (5) homogenizing, cool down rapidly, get product.
Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage prepared by the present embodiment, possesses the fragrant strong perfume (or spice) with Pleurotus eryngii of lactic acid bacteria fermentation Gas, flavour are coordinated soft, sour and sweet palatability, and its total acid content 2.26g/L(is in terms of lactic acid), soluble solid (20 DEG C of refractometers Method) 15.7%, salinity 0.40%.
Embodiment 3:
A kind of preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage, step is as follows,
(1) prepared by Rhizoma Dioscoreae pickle juice: fresh Chinese yam (bio tech ltd of BDCom, Jiangsu) 100kg cleans up, drains the water Point, it is cut into 2 ~ 4cm segment, puts into the pickle jar crossed with 75% alcohol disinfecting in advance, add brewed liquid, add Lactobacillus plantarum JP-8 leaven 4kg(inoculum concentration is the 4% of Rhizoma Dioscoreae quality), build altar lid, pickle jar mouth adds water-stop, is placed in 15 DEG C of environment After fermentation 11d, Pickles are taken out processing and packing separately, altar remains pickled vegetable liquid adds the dilution of 100L tap water, standby;
Described brewed liquid is added 100L certainly by a certain amount of Sal, white sugar, inulin (Fengning safety high-tech Industrial Co., Ltd.), Rhizoma Zingiberis Water mixes, 90 DEG C of insulation 30min, is cooled to 30 DEG C and makes, wherein the mass body of Sal, white sugar, inulin, Rhizoma Zingiberis and water Long-pending ratio respectively 50g/L, 30 g/L, 20 g/L, 10g/L;
(2) Fructus Fragariae Ananssae slurry preparation: fresh for 100kg peaceful beautiful Fructus Fragariae Ananssae (plant science base, Lishui, Jiangsu Province Agriculture Science Institute) is cleaned dry Only, using single track beater making beating (Shanghai Jing Sen light industry and machinery company limited), gained Fructus Fragariae Ananssae slurry is standby;
(3) prepared by pleurotus eryngii juice: after Pleurotus eryngii leftover pieces (bio tech ltd of BDCom, Jiangsu) 75kg cleans up, cut Sheet, adds blanching 5min in the boiling water of 150kg, after being cooled to 30 DEG C by this mushroom aqueous mixtures spiral juice extractor (in Xinxiang City Light Machinery Co., Ltd.) squeezing extracting juice, standby;
(4) preparation of composite juice and enzymolysis: Rhizoma Dioscoreae pickle juice 200kg, Fructus Fragariae Ananssae slurry 100kg, pleurotus eryngii juice 150kg are put into enzymolysis Tank, mix homogeneously, add by add by add by 2:1.2 cellulase (Rui Yong bio tech ltd, Shanghai, 400u/mg) the compound enzymic preparation 67.5g formed with pectase (Rui Yong bio tech ltd, Shanghai, 500u/mg) (0.015%), 65 DEG C of insulation enzymolysis 2h;
(5) centrifugal: the tube centrifuge (sky, PVG this centrifugal machine company limited) of the composite juice after enzymolysis is centrifuged 20min, gained clarified compound juice adds in material-compound tank standby;
(6) allotment: add Mel, xylitol, vitamin C, complex stabilizer, stirring and dissolving in material-compound tank.Clarified compound juice Addition 35g/L of middle Mel, addition 100g/L of xylitol, ascorbic addition is 4.5g/L, and complex stabilizer adds Entering amount is 0.6g/L.Described complex stabilizer is made up of the component of following mass percent: (Zhengzhou height grinds biotechnology to be had pectin Limit company) 22%, xanthan gum (Zibo Deosen Biochemical Ltd.) 32%, sodium carboxymethyl cellulose (Shen, Shanghai light edible chemical Company limited) 46%.
(7) homogenizing: pump into after chuck heating tank is heated to 65 DEG C after step (4) is deployed, through high pressure homogenizer (Sheng Tongji Tool company limited) process 2 times, homogenization pressure is 20MPa.
(8) fill, sterilization: direct fill, 85 DEG C of sterilizing 20min after step (5) homogenizing, cool down rapidly, get product.
Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage prepared by the present embodiment, possesses the fragrant strong perfume (or spice) with Pleurotus eryngii of lactic acid bacteria fermentation Gas, flavour are coordinated soft, sour and sweet palatability, and its total acid content 2.56g/L(is in terms of lactic acid), soluble solid (20 DEG C of refractometers Method) 16.2%, salinity 0.48%.
It should be noted that above-described embodiment, be not used for limiting protection scope of the present invention, in technique scheme Equivalents or replacement done by the basis of each fall within the scope that the claims in the present invention are protected.

Claims (10)

1. the preparation method of a Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage, it is characterised in that described method includes walking as follows Rapid:
(1) preparing Rhizoma Dioscoreae pickle juice: Rhizoma Dioscoreae is cleaned, cutting, segment length 2 ~ 4cm puts into the Pickles crossed in advance with 75% alcohol disinfecting Altar, adds the brewed liquid accounting for Rhizoma Dioscoreae quality 1 ~ 1.5 times, inoculating lactic acid bacterium leavening agent, builds altar lid, and pickle jar mouth adds water-stop, Being placed in 10 ~ 25 DEG C of environment fermentation certain time, take out Rhizoma Dioscoreae Pickles and process separately after fermentation ends, residue juice such as adds at the body Long-pending tap water dilution, obtains Rhizoma Dioscoreae pickle juice;
(2) prepare Fructus Fragariae Ananssae slurry: cleaned up by fresh strawberry, use the making beating of single track beater, obtain Fructus Fragariae Ananssae slurry, standby;
(3) pleurotus eryngii juice is prepared: after being cleaned up by Pleurotus eryngii leftover pieces, section, sheet thickness 1 ~ 2cm, puts into the boiling water of 2 times of quality Middle blanching 5 ~ 10min, squeezes extracting juice by this mushroom aqueous mixtures pressafiner after being cooled to 30 ~ 40 DEG C, obtains pleurotus eryngii juice;
(4) preparation of composite juice, enzymolysis: Rhizoma Dioscoreae pickle juice, Fructus Fragariae Ananssae slurry, pleurotus eryngii juice are proportionally added into enzymatic vessel, and mixing is all Even, obtain composite juice, composite juice adds compound enzymic preparation and carries out enzymolysis;
(5) centrifugal: composite juice enzymolysis filters 15 ~ 20min with tube centrifuge after terminating, and removes filtering residue, obtains clarified compound juice;
(6) allotment: adding Mel, xylitol, vitamin C, complex stabilizer in clarified compound juice, stirring is to being completely dissolved;
(7) homogenizing: the liquid after allotment is put into and carries out homogenizing with high pressure homogenizer after chuck heating tank is heated to 65 ~ 75 DEG C, Homogenization pressure is 20 ~ 22MPa, and homogenization cycles is 2 times;
(8) fill, sterilization: direct fill, sterilizing after homogenizing, cool down, case, obtain Rhizoma Dioscoreae Pleurotus eryngii composite flavor beverage.
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 1, it is characterised in that described In step (1), described brewed liquid is added tap water mixing, 90 ~ 95 DEG C of insulations by a certain amount of Sal, white sugar, inulin, Rhizoma Zingiberis 30min, is cooled to 30 ~ 35 DEG C and makes, and wherein Sal, white sugar, inulin, Rhizoma Zingiberis are respectively 50g/ with the mass volume ratio of water L、30 g/L、20~50 g/L、10~20g/L。
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 1, it is characterised in that described In step (1), the inoculum concentration of leaven is the 2% ~ 5% of Rhizoma Dioscoreae quality, and fermentation time is 11 ~ 14d.
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 1, it is characterised in that described In step (2), described strawberry cultivars is the precocious strawberry cultivars " Ning Yu " of Jiangsu Province Agriculture Science Institute selection-breeding.
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 1, it is characterised in that described In step (4), Rhizoma Dioscoreae pickle juice, Fructus Fragariae Ananssae slurry, the volume ratio of pleurotus eryngii juice are 2:1:1 ~ 2.
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 1, it is characterised in that described In step (4), described compound enzymic preparation is made up of cellulase and pectase, the enzyme activity >=400u/mg of its cellulase, Enzyme activity >=the 500u/mg of pectase, cellulase is 2:1 ~ 1.5 with the mass ratio of pectase, compound enzymic preparation in composite juice Addition be 0.01% ~ 0.015%;Enzymatic hydrolysis condition is: temperature 60 ~ 65 DEG C, enzymolysis time 2 ~ 2.5h.
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 1, it is characterised in that described In step (6), in clarified compound juice the addition of Mel be 20 ~ 40g/L, the addition of xylitol be 60 ~ 100g/L, vitamin C addition is 3.5 ~ 6g/L, the addition of complex stabilizer is 0.6 ~ 1.2g/L.
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 8, it is characterised in that step (6) in, described complex stabilizer is made up of the component of following mass percent: pectin 18% ~ 25%, xanthan gum 26% ~ 32%, carboxylic first Base sodium cellulosate 45% ~ 50%.
9. according to the preparation method of the Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage described in claim 1 ~ 9, it is characterised in that step Suddenly in (8): 85 ~ 90 DEG C of sterilizing 20 ~ 25min.
The preparation method of Rhizoma Dioscoreae Fructus Fragariae Ananssae Pleurotus eryngii composite flavor beverage the most according to claim 10, it is characterised in that step Suddenly, in (1), described lactic acid bacteria is Lactobacillus plantarum JP-8, and it is general that this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms Logical Culture Collection, culture presevation number is CGMCC No.8289, preservation date JIUYUE 29 in 2013.
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