CN106066400A

CN106066400A - A kind of fluorescence immune chromatography test paper detecting 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone

Info

Publication number: CN106066400A
Application number: CN201610424509.8A
Authority: CN
Inventors: 职爱民; 贾国超; 李镁娟; 张培蕾; 周志友; 赵强; 王自良; 宋莲军; 邱国庆
Original assignee: Jiaozuo Baiaotaike Biotech Co Ltd
Current assignee: Jiaozuo Baiaotaike Biotech Co Ltd
Priority date: 2016-06-14
Filing date: 2016-06-14
Publication date: 2016-11-02
Anticipated expiration: 2036-06-14
Also published as: CN106066400B

Abstract

The invention discloses a kind of fluorescence immune chromatography test paper detecting 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone, including supporter and the adsorption layer being fixed on supporter, adsorption layer starts to be followed successively by adsorbing fiber layer, fluorescent antibody fibrous layer, cellulose membrane layer and absorbent material layer from test lead, and described cellulose membrane layer is provided with the stealthy detection trace printed with the carrier protein solution of coupling ZEN and the stealthy comparison trace printed with goat anti-mouse igg, rabbit anti-mouse IgG or goat anti-rabbit igg antibody solution；Described fluorescent antibody fibrous layer uses the glass fibre cotton of absorption fluorescent antibody to make, and fluorescent antibody is graphene oxide fluorescent nano material, NaYF₄: Yb, Tm nano-particle or NaGd (WO₄)₂: Eu³⁺The ZEN monoclonal antibody of nanoparticle label or polyclonal antibody.The test strips of the present invention has high specificity, highly sensitive, stability is high, safety feature good, easy, quick, can realize in situ quantitation detection, can meet the needs of different levels personnel under Portable fluorescence readout instrument.

Description

A kind of fluorescence immune chromatography test paper detecting 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone

Technical field

The present invention relates to a kind of immune chromatography test paper, particularly relate to a kind of fluorescence immunoassay layer detecting 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone Analysis reagent paper.

Background technology

6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone (Zearalenone claims ZEN) is also known as F-2 toxin, mainly by Fusarlum roseum and cereal sickle A kind of non-steroidal mycotoxin that cutter bacterium produces.Be widely present in the Semen Maydis that goes mouldy, Sorghum vulgare Pers., Semen Tritici aestivi, Herba bromi japonici, the frumentum such as Fructus Hordei Vulgaris are made In thing and milk, it is a kind of fusarium toxin that pollution range is the widest in the world, ZEN detection in the feedstuff in the whole nation and feedstuff Rate is up to 100%.ZEN is the biotoxin of a kind of specific toxicity, has estrogen-like effect, animal can be caused to miscarry, extremely Tire, to return the Reproductive Performance such as feelings abnormal, it is also possible to causing growing decline, immunosuppressant, sterile, lopsided etc., ZEN is also by food Chain causes accumulation in human body, and then brings huge economic loss to every year food industry, feed industry and animal husbandry, also gives Human health causes harm greatly, therefore carries out effective quickly detection highly significant for ZEN.China's standard GB/T 13078 regulations, ZEN MRL≤500 μ g/kg in feedstuff and Semen Maydis, the detection method being currently used for ZEN mainly has Biological detection method, chemical analysis, instrumental method and the big class of immunoassay 4.The advantage of biological detection method is measuring samples Being not required to the purest, shortcoming is that sensitivity is low, experimental period is longer.Chemical analysis is a little economical and practical, but can not accurately determine Amount, and the repeatability of analysis result and repeatability poor.Instrumental method has high score and from, high detection usefulness and quickly analyzes energy The advantages such as power, but the pre-treatment to sample requires height, operator's technology is required height, and instrument and equipment is expensive, uncomfortable In quickly detection.Immunoassay is simple to operate, low cost, and has preferable accuracy and susceptiveness, can carry out simultaneously Quantitative and semi-quantitative or the most quantitative detection, be suitable for the examination to a large amount of samples, is the detection technique first developed at present.

The graphene oxide of unmodified shows the most weak fluorescence, does not the most substantially observe under ultraviolet light irradiates Fluorescence.This is owing to there are the epoxy bond on a lot of oxy radical, such as nanometer sheet surface and hydroxyl in stannic oxide/graphene nano sheet surface Base, the carboxyl of nanometer sheet side, the non-radiative recombination in these groups generally energy photoinduced electron-hole pair, thus cause aoxidizing stone The fluorescence of ink alkene is the most weak.After n-butylamine is modified, epoxy bond and the carboxyl on stannic oxide/graphene nano sheet surface are all reacted Falling, this will greatly reduce its non-radiative recombination ability.Therefore, after modifying, graphene oxide shows the strongest fluorescence can conduct Novel markings thing is applied in field of biological detection.Although colloid gold test paper is applied relatively wide in this regard, but it can not be accomplished quantitatively Detection, as the research of more sensitive, stable, flexible, safe graphene oxide fluorescent chromatographic reagent paper, is colloid gold immune detection The strong of technology supplements.

Upper conversion fluorescent nano particle is modified and after activation through surface, as novel markings thing in fields such as biological detection Apply.Because of physical arrangement and the optical characteristics of its uniqueness, make up-conversion fluorescence immune test paper method and Physico-chemical tests and micro-life Analyte detection technology is compared, and has highly sensitive, and operating process is simple, low cost and can carry out the feature of mass field detection； Compared with colloid gold test paper, possess the characteristic of detection by quantitative, as the research of more sensitive, stable, flexible, safe UPT-LF, It is that the strong of colloid gold immune detection technique supplements.But the preparation of upconverting fluorescent material at present and the research of reagent paper still suffer from The defects such as luminous efficiency is inadequate, and fluorescent material kind is few, the research of assistant officer's this aspect to be strengthened and discussion.

Down-conversion fluorescent nano-particle has high sensitivity, is a kind of completely inert phosphor, has uniqueness Physical arrangement and optical characteristics, can apply in field of biological detection as novel markings thing.By itself and biology, immunology, material Material is learned and is combined and the down-conversion fluorescent nanoparticle label immune chromatography method quickly detected for ZEN developed, sensitive, Stable, flexible aspect shows the characteristic of excellence, is that the favourable of colloid gold immune detection technique is supplemented.

Summary of the invention

The technical problem to be solved is to provide a kind of fluorescence immune chromatography test paper detecting 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone, This reagent paper has the features such as special, sensitive, quick, easy, the Gibberella zeae alkene in energy detection by quantitative food, feedstuff, Chinese herbal medicine Ketone.

To achieve these goals, the technical solution adopted in the present invention is:

A kind of fluorescence immune chromatography test paper detecting 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone, including supporter and the suction being fixed on supporter Attached layer, adsorption layer starts to be followed successively by adsorbing fiber layer, fluorescent antibody fibrous layer, cellulose membrane layer and absorbent material from test lead Layer, described cellulose membrane layer is provided with the stealthy detection trace printed with the carrier protein solution of coupling ZEN and little with goat-anti The stealthy comparison trace that Mus IgG, rabbit anti-mouse IgG or goat anti-rabbit igg antibody solution are printed；Described fluorescent antibody fibrous layer is adopted Making with the glass fibre cotton of absorption fluorescent antibody, fluorescent antibody is graphene oxide fluorescent nano material, NaYF₄: Yb, Tm receive Rice grain or NaGd (WO₄)₂: Eu³⁺The ZEN monoclonal antibody of nanoparticle label or polyclonal antibody.

Described supporter includes being arranged on the bottom of adsorption layer bottom surface and being arranged on the surface layer of adsorption layer end face.

Described supporter includes transparent hollow tube, and adsorption layer is filled in inside hollow tube, and adsorption layer is from test End starts to be followed successively by adsorbing fiber layer, fluorescent antibody fibrous layer, cellulose membrane layer and absorbent material layer.

The upper end of described hollow tube is equipped with union joint, and union joint upper end is provided with auxiliary adsorbent equipment, and described is auxiliary Help adsorbent equipment to include with described union joint and in the adapter sleeve being tightly connected and be arranged on the air bag on described adapter sleeve top, air bag Gas outlet be connected with the upper oral part of hollow tube through the inlet channel on adapter sleeve top, the inner chamber of union joint successively, air inlet Being provided with on the adapter sleeve sidewall at passage place can only outwards aerofluxus cannot the unidirectional air-out apparatus of inside air-breathing.

Described unidirectional air-out apparatus includes the ventilation lumen being arranged on the adapter sleeve sidewall at inlet channel place, in ventilation lumen It is provided with the ball sealer of spheroidal, the bottom surface of ventilation lumen has first gas outlet corresponding with ball sealer, the first gas outlet Being connected with inlet channel through outlet passageway, ventilation lumen side is provided with the second gas outlet being connected with ambient atmosphere, constitutes Can only outwards aerofluxus, cannot be to the unidirectional air outlet structure of inlet channel air-breathing.

The first described gas outlet is circular, and its diameter is less than the diameter of ball sealer.

Described union joint is the hollow structure of upper and lower opening, and the upper end of its lower end and hollow tube is in being tightly connected, even Being provided with external screw thread on the outer wall on joint top, the bottom of adapter sleeve is provided with the blind hole corresponding with union joint, blind hole inwall On be provided with the female thread corresponding with external screw thread, union joint is contained in the blind hole of adapter sleeve bottom by external screw thread and internal thread rotation In, on adapter sleeve, it is provided with the sealing ring preventing gas leakage between top and the blind hole top of union joint.

Described stealthy detection trace and stealthy comparison trace are the alternate surface being arranged on cellulose membrane layer, and spacing is 6- 10mm。

The inner chamber of described hollow tube is rectangle.

Described graphene oxide fluorescent nano material be a kind of with graphene oxide for substrate by acyl chloride reaction and After alkylamine is modified, the fluorescent nano material obtained after characterizing with silver nano-grain.

The ZEN monoclonal antibody of described graphene oxide fluorescent nano material labelling or the preparation side of polyclonal antibody Method, comprises the following steps:

(1) graphene oxide fluorescent material is modified:

Take 20mg graphite oxide to grind, join in 5mL DMF, after ultrasonic mixing, add 20mL thionyl chloride, at 80 DEG C It is centrifuged after being heated to reflux 48h, obtains intermediate acid chloride graphene oxide, after washing twice with oxolane, be dried；Taking out again Under conditions of vacuum nitrogen protection, chloride graphene oxide and 1mL n-butylamine are mixed, 60 DEG C of reaction 72h, be cooled to room Temperature, obtain alkylamine modify graphene oxide, be scattered in 20mL distilled water, 8000r/min be centrifuged, remove unreacted just Butylamine, is evaporated remaining reactant liquor by Rotary Evaporators, and the dry after being evaporated is dispersed in distilled water again, is configured to Concentration is the graphene oxide fluorescent material aqueous solution of the modification of 1mg/mL；

(2) preparation of surface markers ZEN antibody silver nanoparticle solution:

A 100mg silver nitrate is dissolved in 250mL ultra-pure water by (), ebuillition of heated, adds the lemon of 10mL mass fraction 1% Lemon acid sodium solution, 80 DEG C are heated to reflux stirring 0.5h after 1h, under the conditions of 4 DEG C overnight；Take 1mL overnight after solution, add 1mM TGA 10 μ L, centrifugal after stirring 24h, remove unreacted TGA, milli-Q water 3 times, steam with Rotary Evaporators Dry, add ultra-pure water and be configured to the silver nanoparticle solution that concentration is 1mg/mL TGA cladding；

B () takes the NHS 10 μ L of EDC 10 μ L and 0.1mM of 0.1mM, be added step-wise to the Yin Na of 1mL TGA cladding In rice grain solution, react 1h, obtain the silver nanoparticle solution of activated carboxylic；

C () takes ZEN monoclonal antibody or the polyclonal antibody 12 μ L of 0.1mM, join the silver nano-grain of activated carboxylic In solution, 4 DEG C of reactions overnight, obtain surface markers ZEN antibody silver nanoparticle solution；

(3) labelling of graphene oxide fluorescent nano material ZEN antibody:

Take the graphene oxide fluorescent material aqueous solution 5mL of modification, add 2mL glutaraldehyde, room temperature reaction 3h, be centrifuged, remove Remove unreacted glutaraldehyde, remaining reactant liquor is dispersed in the PBS buffer solution of 0.01mol/L, pH7.4, add surface Labelling ZEN antibody silver nanoparticle solution, 4 DEG C of reaction 3h, by centrifugation, collect graphene oxide fluorescent nano material labelling ZEN monoclonal antibody or polyclonal antibody, the PBS washing of 0.01mol/L, pH7.4, save backup.

Described NaYF₄: Yb, Tm nano-particle is with NaYF₄For substrate, Yb³⁺For sensitizer, Yb and Tm codope formed A diameter of 60～80nm send out blue-fluorescence nano-particle.

Described NaYF₄: the ZEN monoclonal antibody of Yb, Tm nanoparticle label or the preparation method of polyclonal antibody, Comprise the following steps:

(1) hydrothermal synthesis method is used to prepare NaYF₄: Yb, Tm nano-particle:

Take concentration respectively and be the Y (NO of 0.5mol/L₃)₃Solution 5.5mL, Yb (NO₃)₃Solution 0.5mL and Tm (NO₃)₃Molten Liquid 0.5mL, mix homogeneously, add the sodium citrate solution 2mL of 0.4mol/L, under the conditions of 25 DEG C, lucifuge fully reacts 1h；Add Enter 1.5mol/L NH₄F solution 7mL, under the conditions of 25 DEG C, lucifuge stirring 0.5h, uses HNO₃Regulation pH value, to 7.4, stands 0.5h, adds Distilled water is diluted to 30mL；At 220 DEG C, react 24h again, be cooled to room temperature, through filtration, distilled water washing, be dried, sent out The NaYF of blue light₄: Yb, Tm fluorescent nano particle；

(2) surface siliconization of fluorescent nano particle:

By NaYF₄: Yb, Tm fluorescent nano particle adds in the PBS of 0.01mol/L, pH7.4, is configured to concentration NaYF for 1mg/mL₄: Yb, Tm fluorescent nano particle solution, then 5mL strong aqua ammonia is instilled 5mL NaYF₄: Yb, Tm fluorescence nano In particle solution, it is sufficiently stirred for reacting 20min, adds 2.5mL tetraethyl orthosilicate, under the conditions of 4 DEG C of lucifuges, react 4h； 6000r/min is centrifuged 10min, obtains the fluorescent nano particle of surface siliconization, by washing with alcohol 4 times, is dispersed in methanol, preparation The fluorescent nano particle methanol solution becoming concentration to be 10mg/mL；

(3) surface amination of fluorescent nano particle:

Take 3mL fluorescent nano particle methanol solution, add the acetone of 5mL, magnetic agitation 20min after sealing, then with ultrasonic Ripple is dispersed, adds the APTSA of 3 μ L, reacts 30min at 40 DEG C after sealing, then at 70 DEG C of water-bath 1h, 6000r/min Centrifugal 5min, obtains the fluorescent nano particle of surface amination, and with ethanol cyclic washing 4 times, after vacuum drying, 4 DEG C seal and protect Deposit；

(4) labelling of ZEN antibody:

With the PBS buffer solution of 0.01mol/L, pH7.4, the fluorescent nano particle of surface amination is dissolved, be configured to dense Degree is the fluorescent nano particle solution of the surface amination of 10mg/mL；Take the fluorescent nano particle solution of 10mL surface amination Mixing with 5.6mg NHS and 15mg EDC, room temperature lucifuge reaction 3h, 6000r/min are centrifuged 5min, take supernatant；By 1mg/mL ZEN monoclonal antibody or polyclonal antibody add in supernatant, ZEN monoclonal antibody or polyclonal antibody and supernatant Volume ratio be 1:100,4 DEG C of lucifuges reaction 4h, centrifugal, be dispersed in PBS buffer solution, under the conditions of 4 DEG C after distilled water washing Dialysis 3d, centrifugal, collect precipitation, obtain NaYF₄: the ZEN monoclonal antibody of Yb, Tm nanoparticle label or polyclonal antibody, It is placed in PBS buffer solution, 4 DEG C of preservations.

Described NaGd (WO₄)₂: Eu³⁺Nano-particle is with Gadolinia. (Gd₂O₃), sodium tungstate (Na₂WO₄·2H₂O) it is base Matter, europium ion (Eu³⁺) it is sensitizer, under conditioned response, form the nano-particle of 100～200nm.

Described NaGd (WO₄)₂: Eu³⁺The ZEN monoclonal antibody of nanoparticle label or the preparation side of polyclonal antibody Method, comprises the following steps:

(1) hydrothermal synthesis method is used to prepare NaGd (WO₄)₂: Eu³⁺Nano-particle

Weigh 7g Gd₂O₃With 7g Eu₂O₃, it is added separately to the HNO of 50mL₃In, it being heated to 80 DEG C, insulation is concentrated into all Crystallization, prepares Gd (NO₃)₃With Eu (NO₃)₃；By Na₂WO₄·2H₂O is dissolved in deionized water, and being configured to concentration is 0.2mol/L's Na₂WO₄Solution；By prepared Gd (NO₃)₃With Eu (NO₃)₃With deionized water dissolving, prepare the Gd that mass fraction is 80% respectively (NO₃)₃Solution and the Eu (NO that mass fraction is 15%₃)₃Solution, draws the 5mL Gd (NO of preparation₃)₃Solution, 10mL Na₂WO₄ Solution and 5mL Eu (NO₃)₃Solution, joins in reactor, with dust technology, sodium hydroxide regulation pH=5, stirs, closes Reactor, after 200 DEG C of heated at constant temperature 24h, is down to room temperature naturally；Solution in reactor is poured out standing, treats that in solution, powder body sinks Behind shallow lake, remove supernatant, with distilled water wash powder body 3 times, stand 3h every time；Washed powder body is heated to 80 DEG C of dry 12h, Obtain NaGd (WO₄)₂: Eu³⁺Nano-particle；

(2) labelling of ZEN antibody

ZEN monoclonal antibody or Anti-TNF-α liquid solution 10000r/min are centrifuged 30min, remove impurity；Use deionization Water dissolution NaGd (WO₄)₂: Eu³⁺Nano-particle, is configured to the NaGd (WO that concentration is 0.1mg/mL₄)₂: Eu³⁺Nanoparticles solution, Then 0.1mol/L K is used₂CO₃Solution regulation NaGd (WO₄)₂: Eu³⁺Nanoparticles solution is to pH 8.2；

Take 10mL NaGd (WO₄)₂: Eu³⁺Nanoparticles solution, under stirring, adds ZEN monoclonal antibody or many Clonal antibody solution 100 μ l, ZEN monoclonal antibody or polyclonal antibody solution concentration 1mg/mL, mixing, incubation at room temperature 30min, 4 DEG C, 10000r/min be centrifuged 30min, abandon supernatant, precipitation used 0.02mol/L Na₂B₄O₇Solution is diluted to 10mL, 10000r/ Min is centrifuged 30min, and precipitation uses 0.02mol/L Na₂B₄O₇Solution is diluted to 1mL, and 4 DEG C save backup.

Described adsorbing fiber layer is made up of glass fibre cotton, nylon membrane, PVDF membrane or polyester film.

Described absorbent material layer is made up of absorbent filter.

Described cellulose membrane layer is made up of nitrocellulose filter, pure cellulose film or carboxylated cellulose film.

The carrier protein of described coupling ZEN is bovine serum albumin, chicken egg white or hemocyanin.

Described stealthy detection trace and stealthy comparison trace can also be " 10 " font arrangement trace, " ┬ ┬ " font row Print mark,Font arrangement trace,Font arrangement trace orFont arrangement trace.

Described surface layer covers on adsorbing fiber layer, fluorescent antibody fibrous layer and absorbent material layer, at adsorbing fiber layer It is printed with sample mark line, this sample mark line deflection adsorbing fiber layer on the surface layer corresponding with fluorescent antibody fibrous layer intersection Side 0.3～0.7cm.

The test strips of the present invention has high specificity, highly sensitive, stability is high, safety is good, easy, quick, result Show feature vivid, directly perceived, applied widely, easy to carry.In situ quantitation inspection can be realized under Portable fluorescence readout instrument Survey.The needs of different levels personnel can be met, add including specialty chemical examination, customs quarantine control, health quarantine, quality-monitoring, livestock products Work, raiser and consumer individual etc..The present invention has of crucial importance in terms of ensuring food safety, protecting consumer health Meaning, will have obvious economic benefit and social benefit.

Accompanying drawing explanation

Fig. 1 is the front view of the reagent paper of embodiment 4, and in figure, 2 is adsorbing fiber layer, and 3 is fluorescent antibody fibrous layer, and 4 is fine Dimension element film layer, 7 is absorbent material layer, and 14 is surface layer, and 15 is bottom.

Fig. 2 is the top view of the reagent paper of embodiment 4, and in figure, 4 is cellulose membrane layer, and 5 is stealthy detection trace, and 6 is stealthy Comparison trace, 14 is surface layer, and 15 is bottom, and 16 is sample mark line.

Fig. 3 is the front view of the reagent paper of embodiment 5, and in figure, 1 is hollow tube, and 2 is adsorbing fiber layer, and 3 is fluorescent antibody Fibrous layer, 4 is cellulose membrane layer, and 5 is stealthy detection trace, and 6 is stealthy comparison trace, and 7 is absorbent material layer, and 8 is union joint, 9 is the inner chamber of union joint, and 10 is sealing ring, and 11 is unidirectional air-out apparatus, and 12 is air bag, and 13 is adapter sleeve.

Fig. 4 be the A-A of Fig. 3 in sectional view, figure, 1 is hollow tube, and 4 is cellulose membrane layer, and 6 is stealthy comparison trace.

Fig. 5 is the front view of the union joint in Fig. 3, and in figure, 1 is hollow tube, and 8 is union joint.

Fig. 6 is the top view of the union joint in Fig. 3, and in figure, 8 is union joint, and 9 is the inner chamber of union joint.

Fig. 7 is the sectional view of the adapter sleeve in Fig. 3, and in figure, 10 is sealing ring, and 12 is air bag, and 13 is adapter sleeve, and 111 are Ventilation lumen, 112 is the second gas outlet, and 113 is ball sealer, and 114 is the first gas outlet, and 115 is outlet passageway, and 131 is blind hole, 132 is inlet channel.

Detailed description of the invention

Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.

Embodiment 1

The preparation of fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone, specifically include that ZEN artificial antigen preparation, ZEN monoclonal antibody or the preparation of polyclonal antibody, the preparation of graphene oxide fluorescent nano material labelling ZEN antibody, fiber The steps such as the preparation of element film layer and the assembling of immune chromatography test paper.

1, the preparation of coupling ZEN carrier protein

ZEN is carried out coupling with carrier protein, prepares artificial antigen.

The ZEN weighing 10mg is dissolved in 5mL pyridine, lucifuge stirring reaction 12h, is dried up by gained solution nitrogen, adds 5mL Distilled water, extracts 3 times by equal-volume ethyl acetate, aqueous phase discarded, dries up with nitrogen, obtain grease A.Weigh N-hydroxysuccinimidyl Acid imide (NHS) 2.3mg, dicyclohexylcarbodiimide (DCC) 4.1mg Yu 3.18mg A are sufficiently mixed rear ambient temperature overnight, produce White precipitate, discards precipitation after being centrifuged, takes supernatant and obtain B solution.Weigh BSA 5mg, be dissolved in the carbonate buffer solution (pH of 1mL Be 9.6) in C solution, B is dissolved in and is slowly added in C solution, lucifuge be stirred at room temperature reaction overnight.Product is 4 DEG C of stirrings Lower PBS 3d, changes liquid 3～6 times every day, obtains the ZEN-BSA of purification after dialysis.

2, the preparation of anti-ZEN antibody

(1) preparation of monoclonal antibody

Animal immune: with the ZEN carrier protein couplet thing prepared with 30 μ g～50 μ g/ consumption immunity 6～8 week old only Balb/C mice 3～4 times, each immunization interval time 3～5 weeks, determine that antibody titer carries out superpower immunity after meeting the requirements, and Its suppression valency was detected before merging.

Cell merges: after superpower immunity 3～4 days, take a blood sample by hole under immune mouse socket of the eye, separation positive serum；De-neck is lethal, Alcohol-pickled mice 5～10min with 75% is sterilized body surface, aseptic takes its spleen, is shredded by spleen and grinds, through 120 mesh Buddhist nuns Dragon filtered through gauze, 1000r/min is centrifuged 10min, collects splenocyte.By 1 × 10⁸Splenocyte and NS₀Myeloma cell presses 10:1 Ratio mixing, 1000r/min is centrifuged 10min and abandons supernatant, cell precipitate is slowly added in 37 DEG C of water-baths 0.7～ The 50%PEG4000 of 1.0mL, adds in 1min, and front 30s adds 0.1～0.3mL, and middle 15s adds 0.2～0.4mL, and last 15s adds Complete；Then serum-free 1640 culture medium 15mL it is slowly added into, to terminate the effect of PEG, 37 DEG C of water-baths 5～10min, 1000r/ Min is centrifuged 10min and abandons supernatant, is resuspended in HAT Selective agar medium by cell precipitate, and adds in 96 porocyte culture plates (8 ～10 pieces), 100 μ L～200 μ L/ holes, it is placed in 37 DEG C, the CO of 5%₂Incubator is cultivated.

The screening of monoclonal antibody: cultivate 10～14 days, carries out positive hole sizer with indirect elisa method and selects, selection strong positive, Suppression ratio is high, the eugonic hole of cell carries out 3～6 limited dilution clonings and (until cell clone is monoclonal, detects each Individual clone hole titer, suppression valency are basically identical), then amplification culture, set up hybridoma cell strain.Prepared hybridoma The monoclonal antibody of secretion can be reacted with ZEN specifically, and affinity constant reaches 10¹⁰～10¹², light chain subtype is κ or λ, heavy chain Hypotype is IgG₁、IgG_2a、IgG_2b、IgG₃, for the monoclonal antibody of ZEN specific epitope, it is used for preparing graphite oxide The glass fibre cotton of alkene fluorescent nano material traget antibody.

(2) preparation of polyclonal antibody

By ZEN carrier protein couplet thing immunity New Zealand white rabbit, immunizing dose is 200 μ g～500 μ g/ time, dorsal sc Points 4～6 injections.Head exempts from, and the ZEN carrier protein couplet thing prepared with aseptic PBS dissolving, with equivalent Freund's complete adjuvant (FCA) mixing, fully emulsified；Booster immunization, dissolves ZEN carrier protein couplet thing with aseptic PBS, not exclusively helps with equivalent Freund Agent (FIA) mixes, fully emulsified, and head is carried out after exempting from for 2～3 weeks, continuous immunity 4～5 times, every minor tick 2～3 weeks, for the last time After immunity 10～15 days, survey it with ELISA method and determine titer and reach 10⁵Time above, take a blood sample and separate and collect hyper-immune serum.

Extract IgG antibody with saturated ammonium sulfate salting out method, i.e. take 1 portion of hyper-immune serum and add 2 parts of PBS (pH7.2) mixings, add Volume saturated ammonium sulfate solution mixes, and puts 4 DEG C of refrigerator 12h, 4 DEG C, 2500r/min be centrifuged 15min, abandon supernatant, then with appropriate PBS (pH7.2) dissolution precipitation, adds saturated ammonium sulfate solution to final concentration 33%, puts 4 DEG C of refrigerator 2h, 4 DEG C, 2500r/min is centrifuged 15min, abandons supernatant, with appropriate PBS (pH7.2) dissolution precipitation, puts the 4 DEG C of interior PBS of refrigerators (pH7.2) and dialyses 48～72h, middle Change liquid for several times, 4 DEG C, 12000r/min be centrifuged 15min, collect supernatant, obtain the anti-ZEN polyclonal antibody of purification ,-20 DEG C frozen, For preparing graphene oxide fluorescent nano material traget antibody glass fibre cotton.

3, the ZEN monoclonal antibody of graphene oxide fluorescent nano material labelling or the preparation of polyclonal antibody

(1) graphene oxide fluorescent material is modified:

Take 20mg graphite oxide to grind, join in 5mL dimethylformamide (DMF), after ultrasonic mixing, add 20mL bis- Chlorine sulfoxide, centrifugal after being heated to reflux 48h at 80 DEG C, obtain intermediate acid chloride graphene oxide, wash twice with oxolane After, it is dried；Again under conditions of evacuation nitrogen is protected, chloride graphene oxide and 1mL n-butylamine are mixed, 60 DEG C of reactions 72h, is cooled to room temperature, obtains the graphene oxide that alkylamine is modified, is scattered in 20mL distilled water, and 8000r/min is centrifuged, and removes Removing unreacted n-butylamine, be evaporated by Rotary Evaporators by remaining reactant liquor, the dry after being evaporated is dispersed in double again Steam in water, be configured to the graphene oxide fluorescent material aqueous solution of the modification that concentration is 1mg/mL；

(2) preparation of surface markers ZEN antibody silver nanoparticle solution:

B () takes the NHS10 μ of 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) 10 μ L and 0.1mM of 0.1mM L, is added step-wise in the silver nanoparticle solution of 1mL TGA cladding, reacts 1h, obtain the silver nano-grain of activated carboxylic Solution；

(3) labelling of graphene oxide fluorescent nano material ZEN antibody:

4, the fibrolaminar preparation of fluorescent antibody

The ZEN antibody of the graphene oxide fluorescent nano material labelling diluted by 1:100～1:500 is adsorbed in processed glass In cellucotton, 4 DEG C of low-temperature vacuum dryings, prepare the glass fibre cotton of graphene oxide fluorescent nano material traget antibody.

5, the preparation of adsorptive cellulose film layer:

Cellulose membrane layer is made up of celluloid, distinguishes specking ZEN with point sample instrument at the diverse location of cellulose membrane layer Artificial antigen detects trace and rabbit anti-mouse igg antibody (or sheep anti-mouse igg antibody, goat anti-rabbit igg antibody) comparison trace, in 37 DEG C Dry for standby.

6, the assembling of immune chromatography test paper

Start to be attached to successively from test lead by adsorbing fiber layer, fluorescent antibody fibrous layer, cellulose membrane layer, absorbent material layer On bottom with binding agent, then cover upper layer, and be cut into the wide reagent paper of 3-4cm.

7, detection reaction principle

After reagent paper test lead inserts testing sample solution, under siphonage drives, in solution to be measured, ZEN and fluorescence resist Fluorescent antibody in body fibrous layer spreads to cellulose membrane layer together, until to absorbent material layer.

In diffusion process, ZEN to be measured can combine with absorption ZEN antibody-silver nano-grain on graphene oxide, Because of Ag-Ab effect and electrostatic attraction effect, cause ZEN antibody-silver nano-grain to be stripped out from graphene oxide, enter And discharge the fluorescence that graphene oxide is distributed, and and the carrier protein combination of coupling ZEN.Sheep anti-mouse antibody then can be with ZEN Antigen combines, and excites lower stealthy detection trace line (T line) and stealthy comparison trace line (C line) all can occur at 350nm ultraviolet Absworption peak, reads, by fluorescence, the content that bar instrument reads in T line peak value detection by quantitative testing sample in ZEN.Otherwise, nothing in sample During ZEN, then T line and C line do not have ultraviolet absorption peak.

Embodiment 2

In embodiment 2, preparation and the embodiment 1 of immune chromatography test paper are essentially identical, are in place of main difference: fluorescence resists Body is NaYF₄: the ZEN monoclonal antibody of Yb, Tm nanoparticle label or polyclonal antibody.

Take concentration respectively and be the Y (NO of 0.5mol/L₃)₃Solution 5.5mL, Yb (NO₃)₃Solution 0.5mL and Tm (NO₃)₃Molten Liquid 0.5mL, mix homogeneously, it is slow added into the sodium citrate solution 2mL of 0.4mol/L, under the conditions of 25 DEG C, lucifuge is fully reacted 1h；Add 1.5mol/L NH₄F solution 7mL, with under the conditions of stir 0.5h, use HNO₃Regulation pH value, to 7.4, stands 0.5h, adds double Steam water and be diluted to 30mL；At 220 DEG C, react 24h again, be cooled to room temperature, through filtration, distilled water washing, be dried, turned blue The NaYF of coloured light₄: Yb, Tm fluorescent nano particle；

(2) surface siliconization of fluorescent nano particle:

(3) surface amination of fluorescent nano particle:

Take 3mL fluorescent nano particle methanol solution, add the acetone of 5mL, magnetic agitation 20min after sealing, then with ultrasonic Ripple is dispersed, adds N-(2-the aminoethyl)-3-aminopropyl trimethoxysilane (APTSA) of 3 μ L, 40 DEG C of reactions after sealing 30min, then at 70 DEG C of water-bath 1h, 6000r/min is centrifuged 5min, obtains the fluorescent nano particle of surface amination, uses second Alcohol cyclic washing 4 times, after vacuum drying, 4 DEG C seal preservation；

(4) labelling of ZEN antibody:

Detection reaction principle

In diffusion process, ZEN to be measured can combine with fluorescent antibody, and then closes the antigen knot of ZEN in fluorescent antibody Chalaza, stops the detection trace of fluorescent antibody ZEN artificial antigen on cellulose membrane to be combined, it is impossible to display detection trace, and Sheep or rabbit anti-mouse IgG (or goat anti-rabbit igg) antibody then can be combined with fluorescent antibody, read bar by fluorescence under infrared excitation Absworption peak would not occur at instrument T line, at C line, there will be absworption peak.Otherwise time in sample solution without ZEN, then can not stop glimmering Photoactivated antibody detection trace of coupling ZEN carrier protein on cellulose membrane is combined, and reads to arise that suction at bar instrument T line by fluorescence Receiving peak, same goat-anti or rabbit anti-mouse IgG (or goat anti-rabbit igg) antibody also can be combined with fluorescent-labeled antibody, read by fluorescence Also absworption peak is there will be at bar instrument C line.If there is no T line and C line absorption peak on cellulose membrane, then show that test strips lost efficacy. Embodiment 3

In embodiment 3, preparation and the embodiment 1 of immune chromatography test paper are essentially identical, are in place of main difference: fluorescence resists Body is NaGd (WO₄)₂: Eu³⁺The ZEN monoclonal antibody of nanoparticle label or polyclonal antibody.

(2) labelling of ZEN antibody

Detection reaction principle

After reagent paper test lead inserts testing sample solution, solution to be measured drives ZEN to be measured and fluorescence by siphonage Down-conversion fluorescent nano-particle NaGd (WO in nanoparticle label antibody glass fibre cotton₄)₂: Eu³⁺Traget antibody together to Cellulose membrane layer spreads, and eventually penetrates the absorbent material layer of handle end.In diffusion process, ZEN to be measured can be with fluorescence nano Down-conversion fluorescent nanoparticle label antibody in particle marker antibody fibrous layer combines, and then closes down-conversion fluorescent nanometer The antigen-combining site of ZEN on particle marker antibody, stops down-conversion fluorescent nanoparticle label antibody and coupling on cellulose membrane The detection trace of ZEN carrier protein combines, and reagent paper cannot show detection trace, and sheep or rabbit anti-mouse IgG (or goat-anti rabbit IgG) antibody then can read bar instrument T line by fluorescence with down-conversion fluorescent nanoparticle label antibodies under infrared excitation Would not there is absworption peak in place, there will be absworption peak at C line.If otherwise without ZEN in sample solution, then lower conversion can not be stoped glimmering Light nanoparticle label antibody detection trace of coupling ZEN carrier protein on cellulose membrane is combined, and reads bar instrument T line by fluorescence Place arises that absworption peak, same goat-anti or rabbit anti-mouse IgG (or goat anti-rabbit igg) antibody also can be with down-conversion fluorescent nanometers Grain traget antibody combines, and reads also to there will be absworption peak at bar instrument C line by fluorescence.If not having T line and C line to inhale on cellulose membrane Receive peak, then show that test strips lost efficacy.

Structure and the detection method of immune chromatography test paper is illustrated below in conjunction with other embodiments

Embodiment 4

The fluorescence immune chromatography test paper of the detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone of the present embodiment, with reference to Fig. 1-2, including supporter with solid The adsorption layer being scheduled on supporter, adsorption layer starts to be followed successively by adsorbing fiber layer 2, fluorescent antibody fibrous layer 3, fiber from test lead Element film layer 4 and absorbent material layer 7, described cellulose membrane layer is provided with the stealth printed with the carrier protein solution of coupling ZEN Detection trace 5 and the stealthy comparison trace 6 printed with goat anti-mouse igg, rabbit anti-mouse IgG or goat anti-rabbit igg antibody solution；Institute The fluorescent antibody fibrous layer stated uses the glass fibre cotton of absorption fluorescent antibody to make, and fluorescent antibody is that graphene oxide fluorescence is received The ZEN monoclonal antibody of rice material marking or polyclonal antibody.

Described supporter includes the bottom 15 being arranged on adsorption layer bottom surface and is arranged on the surface layer 14 of adsorption layer end face.

Described surface layer covers on adsorbing fiber layer, fluorescent antibody fibrous layer and absorbent material layer, at adsorbing fiber layer It is printed with sample mark line 16, this sample mark line deflection adsorbing fiber on the surface layer corresponding with fluorescent antibody fibrous layer intersection Layer side 0.3～0.7cm.

For superior technique effect, described stealthy detection trace 5 and stealthy comparison trace 6 are arranged on fiber in alternate The surface of element film layer 4, i.e. two trace bands one-tenth arranged in parallel " ∥ ", spacing is 6～10mm.

Covering the surface layer on adsorbing fiber layer and fluorescent antibody fibrous layer is white, covers on absorbent material layer Surface layer be other color (such as yellow), test sample mark line is positioned at adsorbing fiber layer and fluorescent antibody fibrous layer intersection pair At the white surface layer deflection adsorbing fiber layer side about 0.5cm answered, on the right side of mark line, on surface layer, it is printed on arrow and max printed words.

(1) preparation of testing sample and detecting step:

Detection corn-like: by sample comminution, make the sample suspension of 1:10 (m/v) with dehydrated alcohol dilution.

Operational approach: being inserted by ZEN reagent paper test lead in testing sample, insertion depth is less than mark line, about 10～20 Second take out reagent paper, put into after 5min fluorescence read the direct readings of bar instrument.

Result judges:

A () is positive judges: read all to occur at bar instrument T line and C line absworption peak by fluorescence, represents that testing result is positive, Illustrate in testing sample containing ZEN；

B () is negative judges: read all to occur without at bar instrument T line and C line absworption peak by fluorescence, represents that testing result is cloudy Property, illustrate in testing sample without ZEN；

C () fail-ure criterion: read bar instrument T line by fluorescence and occur without absworption peak, occurs occurring at absworption peak, or T line at C line Absworption peak, occurs without absworption peak at C line, then show that reagent paper lost efficacy.

(2) susceptiveness of the present embodiment immune chromatography test paper, specific detection

The detection of susceptiveness: with phosphate buffer PBS (pH 7.4) or distilled water compound concentration respectively be 1,2,3,4, 5,10,20,30, the ZEN standard substance of 50ng/mL, loading 80～100 μ L on the immune chromatography test paper of the present embodiment, reaction After 5min, read relative optical density number (the relative optical of T line scan area optical density by reading bar instrument Density, ROD).With the percentage rate of variable concentrations standard substance and zero standard product relative optical density number as vertical coordinate, with difference The common logarithm value of standard concentration is abscissa, draws standard curve, carries out correlation regression analysis, calculate this reagent paper to ZEN IC₅₀And lowest detectable limit.After measured, the Regression Equations of ZEN pair, this reagent paper is: y=-0.3164x+0.984, phase relation Number is R²=0.996, go out this reagent paper IC to ZEN according to regression equation calculation₅₀For 33.86ng/mL, the lowest detection of this reagent paper It is limited to 4.87ng/mL, shows that immune chromatography test paper has higher sensitivity to ZEN.

Specific detection: using other mycotoxins as competitor, prepares above-mentioned standard substance variable concentrations, with immunity layer Analysis its suppression ratio of detection paper, with this reagent paper to ZEN's and IC₅₀Each competitor IC₅₀Percentage ratio as its cross reacting rate. Measurement result is shown in Table 1.As seen from Table 1, the specificity of this immune chromatography test paper is preferable, all anti-without intersecting with other mycotoxins Should.

Table 1 immune chromatography test paper and the cross reaction of other mycotoxins

The test strips of the present embodiment has the advantage that

(1) high specificity, highly sensitive.The test strips of the present embodiment is with graphene oxide fluorescent nano material labelling Make based on ZEN antibody, owing to the graphene oxide fluorescent material of modified mistake has outstanding optical characteristics and good life The thing compatibility, simultaneous oxidation Graphene electron transmission efficiency is high so that this Novel immune method is highly sensitive, detectable limit Low, minimum only 4.87ng/mL.

(2) stability is high, and safety is good.The ZEN of the test strips graphene oxide fluorescent nano material labelling of the present embodiment Make based on antibody, nano-Ag particles surface with protein with contrary charge groups, the strong bonded because of Electrostatic Absorption； Graphene specific surface area is big, can be combined by chemical reaction with multiple proteins biomolecule, and affects its biological activity very Little, stability is high, and motility is good.

(3) Semen Maydis, DDGS, feedstuff etc. can be detected by the graphene oxide fluorescence immune chromatography test paper of the present embodiment. Jointly being acted on by silver nanoparticle and graphene oxide, detection signal strengthens, and reduces antibody consumption, saves antibody cost.

Embodiment 5

The fluorescence immune chromatography test paper of the detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone of the present embodiment, with reference to Fig. 3-7, including supporter with solid The adsorption layer being scheduled on supporter, described supporter includes transparent hollow tube 1, and adsorption layer is filled in hollow tube Portion, adsorption layer starts to be followed successively by adsorbing fiber layer 2, fluorescent antibody fibrous layer 3, cellulose membrane layer 4 and absorbent material from test lead Layer 7, described cellulose membrane layer is provided with the stealthy detection trace 5 printed with the carrier protein solution of coupling ZEN and uses goat-anti The stealthy comparison trace 6 that mouse IgG, rabbit anti-mouse IgG or goat anti-rabbit igg antibody solution are printed；Described fluorescent antibody fiber Layer uses the glass fibre cotton of absorption fluorescent antibody to make, and fluorescent antibody is NaYF₄: the ZEN Dan Ke of Yb, Tm nanoparticle label Grand antibody or polyclonal antibody.

The upper end of described hollow tube 1 is equipped with union joint 8, and union joint 8 upper end is provided with auxiliary adsorbent equipment, described Auxiliary adsorbent equipment includes with described union joint in the adapter sleeve 13 being tightly connected with the air bag that is arranged on described adapter sleeve top 12, the gas outlet of air bag 12 is successively through the inlet channel 132 on adapter sleeve 13 top, the inner chamber 9 of union joint 8 and hollow tube 1 Upper oral part is connected, the adapter sleeve sidewall at inlet channel place is provided with can only outwards aerofluxus cannot inwardly air-breathing unidirectional go out Device of air 11.

Described unidirectional air-out apparatus includes the ventilation lumen 111 being arranged on the adapter sleeve sidewall at inlet channel place, ventilation It is provided with the ball sealer 113 of spheroidal in chamber 111, the bottom surface of ventilation lumen 111 has first corresponding with ball sealer and gives vent to anger Mouth 114, the first gas outlet is connected with inlet channel 132 through outlet passageway 115, and ventilation lumen 111 side is provided with big with the external world Gas phase connection the second gas outlet 112, constitute can only outwards aerofluxus, cannot be to the unidirectional air outlet structure of inlet channel air-breathing.

For superior technique effect, the first described gas outlet 114 is circular, and its diameter is less than the diameter of ball sealer. Such setting can make the first gas outlet be combined more closely with the ball sealer of spheroidal, it is ensured that the sealing in space.

Described union joint 8 is the hollow structure of upper and lower opening, the upper end of its lower end and hollow tube 1 in being tightly connected, Being provided with external screw thread on the outer wall on union joint top, the bottom of adapter sleeve 13 is provided with obtain blind hole 131 corresponding with union joint, blind Being provided with the female thread corresponding with external screw thread on the inwall of hole 131, union joint is contained in adapter sleeve by external screw thread and internal thread rotation In the blind hole 131 of bottom, on adapter sleeve, between top and the blind hole top of union joint, it is provided with the sealing ring 10 preventing gas leakage.

Described stealthy detection trace 5 and stealthy comparison trace 6 are in the alternate surface being arranged on cellulose membrane layer 4, spacing It is 6～10mm.

The inner chamber of described hollow tube 1 is rectangle.

Test sample mark line is positioned at the clear hollow body that adsorbing fiber layer is corresponding with fluorescent antibody fibrous layer intersection At upper deflection adsorbing fiber layer side about 0.5cm, the clear hollow body on the right side of mark line is printed on arrow and max printed words.

During use, adapter sleeve and union joint are linked together by internal and external threads, gently extruding gasbag, the gas in air bag Body is discharged by the ventilation lumen of inlet channel, the inner chamber of union joint and adapter sleeve sidewall, now the spheroidal in ventilation lumen The gas that ball sealer is squeezed out from air bag lifts, and the gas in air bag is discharged smoothly by the first gas outlet；Will examination Paper inserts in testing sample solution, then unclamps air bag, now, the inner chamber of hollow tube, the inner chamber of union joint and inlet channel Interior generation negative pressure, under the attraction of negative pressure, ball sealer tightly adsorbs on the first gas outlet on the bottom surface of ventilation lumen, by its envelope Close, thus help sample solution more quickly to infiltrate reagent paper, thus reduce the time of sample solution infiltration test strips, improve inspection Survey efficiency.

By above-mentioned situation it should be apparent that the present embodiment novel structure is unique, advantages of simple, by whole by supporter Body is revised as closed structure, increases air bag at the top of supporter simultaneously, makes absorption more rapid, thus is effectively improved detection Speed and accuracy, cell parts and supporter part connect for sealing reassembling type simultaneously, only need to change support after detection Body portion, easy accessibility, easily operate, using effect is good, is the innovation on Test paper.

(1) preparation of testing sample and detecting step:

Detection DDGS sample: by sample comminution, make the sample suspension of 1:10 (m/v) with dehydrated alcohol dilution.

Operational approach: extruding gasbag, discharges the gas in air bag, is inserted by ZEN reagent paper test lead in testing sample, inserts Enter the degree of depth and be less than mark line, unclamp air bag, within about 3-5 second, take out reagent paper, put into fluorescence after 4min and read the direct readings of bar instrument.

Result judges:

A () is positive judges: reads to occur without absworption peak at bar instrument T line by fluorescence, absworption peak occurs, represent detection at C line Result is positive, illustrates in testing sample containing 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone；

B () is negative judges: read all to occur at bar instrument T line and C line absworption peak by fluorescence, represents that testing result is negative, Illustrate in testing sample without 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone；

(c) fail-ure criterion: read all to occur without absworption peak at bar instrument T line and C line by fluorescence, then show that reagent paper lost efficacy.

With phosphate buffer PBS (pH 7.4) or distilled water compound concentration respectively be 1,2,3,4,5,10,20,30, The ZEN standard substance of 50ng/mL, loading 80～100 μ L on the immune chromatography test paper of the present embodiment, after reaction 4min, by reading Bar instrument reads the relative optical density number (relative optical density, ROD) of T line scan area optical density.With difference Concentration standards is vertical coordinate with the percentage rate of zero standard product relative optical density number, with the common logarithm of various criterion product concentration Value is abscissa, draws standard curve, carries out correlation regression analysis, calculate this reagent paper IC to ZEN₅₀And lowest detectable limit.Warp Measuring, the Regression Equations of ZEN pair, this reagent paper is: y=-0.4134x+0.8893, and correlation coefficient is R²=0.996, according to Regression equation calculation goes out this reagent paper IC to ZEN₅₀For 44.86ng/mL, the lowest detection of this reagent paper is limited to 5.78ng/mL, shows Immune chromatography test paper has higher sensitivity to ZEN.

Specific detection: using other mycotoxins as competitor, prepares above-mentioned standard substance variable concentrations, with immunity layer Analysis its suppression ratio of detection paper, with this reagent paper to ZEN's and IC₅₀Each competitor IC₅₀Percentage ratio as its cross reacting rate. Measurement result is shown in Table 2.As seen from Table 2, the specificity of this immune chromatography test paper is preferable, all anti-without intersecting with other mycotoxins Should.

Table 2 immune chromatography test paper and the cross reaction of other mycotoxins

Compound	Half-inhibition concentration IC₅₀(ng/mL)	Cross reacting rate (%)
			6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone	44.86	100
Ochratoxin A	＞ 1.0 × 10⁵	＜ 0.045
			Vomitoxin	＞ 1.0 × 10⁵	＜ 0.045
Fumonisin B₁	＞ 1.0 × 10⁵	＜ 0.045
			T-2 toxin	＞ 1.0 × 10⁵	＜ 0.045
Aflatoxin B₁	＞ 1.0 × 10⁵	＜ 0.045

The test strips of the present embodiment has the advantage that

(1) high specificity, highly sensitive.The present embodiment up-conversion fluorescence immune chromatography test paper is to turn blue spectrochrome upper turn Change fluorescent nano material NaYF₄: make, due to NaYF based on the ZEN antibody of Yb, Tm labelling₄: Yb, Tm nano-particle luminescence is imitated Rate is high, can effectively eliminate sample detection bias light, and sensitivity is greatly improved.

(2) stability is high, and safety is good.Upconversion fluorescence nano material NaYF in the reagent paper of the present embodiment₄: Yb, Tm energy Effectively launch blue color spectrum, it is entirely avoided the luminous cancellation that other conditions cause.NaYF₄: Yb, Tm material has inorganic lazy Property, infrared ray excited, the feature of VISIBLE LIGHT EMISSION, use this reagent paper to carry out detecting for surrounding people with environment without any danger Evil.

(3) easy, quickly.Utilizing fluorescence to read bar instrument, this reagent paper can direct readings, it is achieved scene Quantitative detection.Only Reagent paper is inserted test sample 3～5 seconds, i.e. can read result after 4 minutes, at T line, represent test sample without absworption peak In containing ZEN；Otherwise, without ZEN.Visual result, accurately, simple and clear, avoid artificial erroneous judgement to greatest extent.

(4), when prepared by the reagent paper of the present embodiment, fluorescent antibody uses NaYF₄: the ZEN Dan Ke of Yb, Tm nanoparticle label Grand antibody or polyclonal antibody, will amidized NaYF₄: Yb, Tm nano-particle is directly connected with antibody, labeling process letter Single, Conjugate ratio is high, thus is effectively improved based on NaYF₄: the sensitivity of the immune test paper of Yb, Tm material.

Embodiment 6

The structure of the fluorescence immune chromatography test paper of the detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone of the present embodiment is with embodiment 4, difference , the fluorescent antibody of the present embodiment is NaGd (WO₄)₂: Eu³⁺The ZEN monoclonal antibody of nanoparticle label or Anti-TNF-α Body.

(1) preparation of testing sample and detecting step:

Result judges:

With phosphate buffer PBS (pH 7.4) or distilled water compound concentration respectively be 1,2,3,4,5,10,20,30, The ZEN standard substance of 50ng/mL, loading 80～100 μ L on the immune chromatography test paper of the present embodiment, after reaction 4min, by reading Bar instrument reads the relative optical density number (relative optical density, ROD) of T line scan area optical density.With difference Concentration standards is vertical coordinate with the percentage rate of zero standard product relative optical density number, with the common logarithm of various criterion product concentration Value is abscissa, draws standard curve, carries out correlation regression analysis, calculate this reagent paper IC to ZEN₅₀And lowest detectable limit.Warp Measuring, the Regression Equations of ZEN pair, this reagent paper is: y=-0.3467x+0.8841, and correlation coefficient is R²=0.987, according to Regression equation calculation goes out this reagent paper IC to ZEN₅₀For 45.8ng/mL, the lowest detection of this reagent paper is limited to 5.36ng/mL, shows Immune chromatography test paper has higher sensitivity to ZEN.

Specific detection: using other mycotoxins as competitor, prepares above-mentioned standard substance variable concentrations, with immunity layer Analysis its suppression ratio of detection paper, with this reagent paper to ZEN's and IC₅₀Each competitor IC₅₀Percentage ratio as its cross reacting rate. Measurement result is shown in Table 3.As seen from Table 3, the specificity of this immune chromatography test paper is preferable, all anti-without intersecting with other mycotoxins Should.

Table 3 immune chromatography test paper and the cross reaction of other mycotoxins

Compound	Half-inhibition concentration IC₅₀(ng/mL)	Cross reacting rate (%)
			6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone	45.8	100
Ochratoxin A	＞ 1.0 × 10⁵	＜ 0.046
			Vomitoxin	＞ 1.0 × 10⁵	＜ 0.046
Fumonisin B₁	＞ 1.0 × 10⁵	＜ 0.046
			T-2 toxin	＞ 1.0 × 10⁵	＜ 0.046
Aflatoxin B₁	＞ 1.0 × 10⁵	＜ 0.046

The test strips of the present embodiment has the advantage that

(1) high specificity, highly sensitive.The present embodiment down-conversion fluorescent nanoparticle label immune chromatography test paper is with oxygen Change gadolinium (Gd₂O₃), sodium tungstate (Na₂WO₄·2H₂O) it is substrate, europium ion (Eu³⁺) be sensitizer formed 100～200nm nanometers Granule, has good optical characteristics, makes the detection of ZEN eliminate the interference of bias light, and spirit lightness is greatly improved, and minimum examines Measure 5.36ng/mL.

(2) stability is high.NaGd(WO₄)₂: Eu³⁺Nano-particle belongs to completely inert phosphor, these character Make it have extraordinary light stability, photobleaching does not occur under the irradiation of uviol lamp so that readings is stable.

(3) easy, quickly.Use the present embodiment reagent paper can read bar instrument with fluorescence to be used in combination, direct readings, it is achieved on-the-spot Detection by quantitative, only need to insert test strips in test sample 3-5 second, i.e. can determine that testing result in taking out latter 4 minutes.

(4) result display is vivid, directly perceived, accurately.The present embodiment fluorescent nano particle labeled immunochromatographyassay assay test is with T line Whether the standard that absworption peak is positive and negative as detection occurs.If without absworption peak at T line, representing in test sample and contain ZEN, on the contrary then represent in test sample without ZEN.Visual result, accurately, is difficult to occur that false positive and false negative etc. are the most by mistake Sentence.

Claims

1. detect a fluorescence immune chromatography test paper for 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone, including supporter and the absorption being fixed on supporter Layer, adsorption layer starts be followed successively by adsorbing fiber layer (2), fluorescent antibody fibrous layer (3), cellulose membrane layer (4) and inhale from test lead Water material layer (7), it is characterised in that described cellulose membrane layer is provided with the hidden of the carrier protein solution printing of use coupling ZEN Shape detection trace (5) and the stealthy comparison trace printed with goat anti-mouse igg, rabbit anti-mouse IgG or goat anti-rabbit igg antibody solution (6)；Described fluorescent antibody fibrous layer uses the glass fibre cotton of absorption fluorescent antibody to make, and fluorescent antibody is graphene oxide Fluorescent nano material, NaYF₄: Yb, Tm nano-particle or NaGd (WO₄)₂: Eu³⁺The ZEN monoclonal antibody of nanoparticle label or Person's polyclonal antibody.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 1, it is characterised in that described Supporter includes the bottom (15) being arranged on adsorption layer bottom surface and is arranged on the surface layer (14) of adsorption layer end face.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 1, it is characterised in that described Supporter includes transparent hollow tube (1), and adsorption layer is filled in inside hollow tube, and adsorption layer starts to be followed successively by from test lead Adsorbing fiber layer, fluorescent antibody fibrous layer, cellulose membrane layer and absorbent material layer.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 3, it is characterised in that described The upper end of hollow tube (1) is equipped with union joint (8), and union joint (8) upper end is provided with auxiliary adsorbent equipment, described auxiliary absorption Device includes with described union joint in the adapter sleeve (13) being tightly connected with the air bag (12) being arranged on described adapter sleeve top, gas The gas outlet of capsule (12) is successively through the inlet channel (132) on adapter sleeve (13) top, the inner chamber (9) of union joint (8) and hollow pipe The upper oral part of body (1) is connected, and the adapter sleeve sidewall at inlet channel place is provided with can only outwards aerofluxus cannot inside air-breathing Unidirectional air-out apparatus (11)；

Described unidirectional air-out apparatus includes the ventilation lumen (111) being arranged on the adapter sleeve sidewall at inlet channel place, ventilation lumen (111) it is provided with the ball sealer (113) of spheroidal in, the bottom surface of ventilation lumen (111) has first corresponding with ball sealer Gas outlet (114), the first gas outlet is connected with inlet channel (132) through outlet passageway (115), and ventilation lumen (111) side sets Be equipped with the second gas outlet (112) being connected with ambient atmosphere, constitute can only outwards aerofluxus, cannot be to the list of inlet channel air-breathing To air outlet structure.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 4, it is characterised in that described First gas outlet (114) is circular, and its diameter is less than the diameter of ball sealer.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 4, it is characterised in that described Union joint (8) is the hollow structure of upper and lower opening, the upper end of its lower end and hollow tube (1) in being tightly connected, union joint top Outer wall on be provided with external screw thread, the bottom of adapter sleeve (13) is provided with the blind hole (131) corresponding with union joint, blind hole (131) being provided with the female thread corresponding with external screw thread on inwall, union joint is contained in adapter sleeve by external screw thread and internal thread rotation In the blind hole (131) of bottom, on adapter sleeve, between top and the blind hole top of union joint, it is provided with the sealing ring preventing gas leakage (10)。

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 1, it is characterised in that described Stealthy detection trace (5) and stealthy comparison trace (6) are in the alternate surface being arranged on cellulose membrane layer (4), and spacing is 6-10mm；

The inner chamber of described hollow tube (1) is rectangle.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 1, it is characterised in that described The ZEN monoclonal antibody of graphene oxide fluorescent nano material labelling or the preparation method of polyclonal antibody, including following step Rapid:

(1) graphene oxide fluorescent material is modified:

Take 20mg graphite oxide to grind, join in 5mL DMF, after ultrasonic mixing, add 20mL thionyl chloride, heat at 80 DEG C It is centrifuged after backflow 48h, obtains intermediate acid chloride graphene oxide, after washing twice with oxolane, be dried；Again at evacuation Under conditions of nitrogen protection, chloride graphene oxide and 1mL n-butylamine are mixed, 60 DEG C of reaction 72h, be cooled to room temperature, The graphene oxide modified to alkylamine, is scattered in 20mL distilled water, and 8000r/min is centrifuged, and removes unreacted n-butylamine, Being evaporated by Rotary Evaporators by remaining reactant liquor, the dry after being evaporated is dispersed in distilled water again, is configured to concentration Graphene oxide fluorescent material aqueous solution for the modification of 1mg/mL；

(2) preparation of surface markers ZEN antibody silver nanoparticle solution:

A 100mg silver nitrate is dissolved in 250mL ultra-pure water by (), ebuillition of heated, adds the citric acid of 10mL mass fraction 1% Sodium solution, 80 DEG C are heated to reflux stirring 0.5h after 1h, under the conditions of 4 DEG C overnight；Take 1mL overnight after solution, add 1mM sulfydryl Acetic acid 10 μ L, centrifugal after stirring 24h, remove unreacted TGA, milli-Q water 3 times, be evaporated with Rotary Evaporators, add Ultra-pure water is configured to the silver nanoparticle solution that concentration is 1mg/mL TGA cladding；

B () takes the NHS 10 μ L of EDC 10 μ L and 0.1mM of 0.1mM, be added step-wise to the silver nanoparticle of 1mL TGA cladding In grain solution, react 1h, obtain the silver nanoparticle solution of activated carboxylic；

C () takes ZEN monoclonal antibody or the polyclonal antibody 12 μ L of 0.1mM, join the silver nanoparticle solution of activated carboxylic In, 4 DEG C of reactions overnight, obtain surface markers ZEN antibody silver nanoparticle solution；

(3) labelling of graphene oxide fluorescent nano material ZEN antibody:

Taking the graphene oxide fluorescent material aqueous solution 5mL of modification, add 2mL glutaraldehyde, room temperature reaction 3h, centrifugal, removing is not The glutaraldehyde of reaction, is dispersed in remaining reactant liquor in the PBS buffer solution of 0.01mol/L, pH7.4, adds surface markers ZEN antibody silver nanoparticle solution, 4 DEG C of reaction 3h, by centrifugation, the ZEN collecting graphene oxide fluorescent nano material labelling is mono- Clonal antibody or polyclonal antibody, the PBS washing of 0.01mol/L, pH7.4, save backup.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 1, it is characterised in that described NaYF₄: the ZEN monoclonal antibody of Yb, Tm nanoparticle label or the preparation method of polyclonal antibody, comprise the following steps:

Take concentration respectively and be the Y (NO of 0.5mol/L₃)₃Solution 5.5mL, Yb (NO₃)₃Solution 0.5mL and Tm (NO₃)₃Solution 0.5mL, mix homogeneously, add the sodium citrate solution 2mL of 0.4mol/L, under the conditions of 25 DEG C, lucifuge fully reacts 1h；Add 1.5mol/L NH₄F solution 7mL, under the conditions of 25 DEG C, lucifuge stirring 0.5h, uses HNO₃Regulation pH value, to 7.4, stands 0.5h, adds double Steam water and be diluted to 30mL；At 220 DEG C, react 24h again, be cooled to room temperature, through filtration, distilled water washing, be dried, turned blue The NaYF of coloured light₄: Yb, Tm fluorescent nano particle；

(2) surface siliconization of fluorescent nano particle:

By NaYF₄: Yb, Tm fluorescent nano particle adds in the PBS of 0.01mol/L, pH7.4, and being configured to concentration is 1mg/ The NaYF of mL₄: Yb, Tm fluorescent nano particle solution, then 5mL strong aqua ammonia is instilled 5mL NaYF₄: Yb, Tm fluorescent nano particle is molten In liquid, it is sufficiently stirred for reacting 20min, adds 2.5mL tetraethyl orthosilicate, under the conditions of 4 DEG C of lucifuges, react 4h；6000r/min Centrifugal 10min, obtains the fluorescent nano particle of surface siliconization, by washing with alcohol 4 times, is dispersed in methanol, and being configured to concentration is The fluorescent nano particle methanol solution of 10mg/mL；

(3) surface amination of fluorescent nano particle:

Take 3mL fluorescent nano particle methanol solution, add the acetone of 5mL, magnetic agitation 20min after sealing, more equal with ultrasound wave Even dispersion, adds the APTSA of 3 μ L, reacts 30min at 40 DEG C after sealing, more centrifugal at 70 DEG C of water-bath 1h, 6000r/min 5min, obtains the fluorescent nano particle of surface amination, and with ethanol cyclic washing 4 times, after vacuum drying, 4 DEG C seal and preserve；

(4) labelling of ZEN antibody:

Being dissolved by the fluorescent nano particle of surface amination with the PBS buffer solution of 0.01mol/L, pH7.4, being configured to concentration is The fluorescent nano particle solution of the surface amination of 10mg/mL；Take the fluorescent nano particle solution of 10mL surface amination with 5.6mg NHS and 15mg EDC mixing, room temperature lucifuge reaction 3h, 6000r/min are centrifuged 5min, take supernatant；By 1mg/mL's ZEN monoclonal antibody or polyclonal antibody add in supernatant, ZEN monoclonal antibody or polyclonal antibody and supernatant Volume ratio is 1:100,4 DEG C of lucifuge reaction 4h, centrifugal, is dispersed in PBS buffer solution, under the conditions of 4 DEG C thoroughly after distilled water washing Analysis 3d, centrifugal, collect precipitation, obtain NaYF₄: the ZEN monoclonal antibody of Yb, Tm nanoparticle label or polyclonal antibody, put In PBS buffer solution, 4 DEG C of preservations.

The fluorescence immune chromatography test paper of detection 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone the most according to claim 1, it is characterised in that described NaGd (WO₄)₂: Eu³⁺The ZEN monoclonal antibody of nanoparticle label or the preparation method of polyclonal antibody, including following Step:

Weigh 7g Gd₂O₃With 7g Eu₂O₃, it is added separately to the HNO of 50mL₃In, it being heated to 80 DEG C, insulation is concentrated into all knots Crystalline substance, prepares Gd (NO₃)₃With Eu (NO₃)₃；By Na₂WO₄·2H₂O is dissolved in deionized water, and being configured to concentration is 0.2mol/L's Na₂WO₄Solution；By prepared Gd (NO₃)₃With Eu (NO₃)₃With deionized water dissolving, prepare the Gd that mass fraction is 80% respectively (NO₃)₃Solution and the Eu (NO that mass fraction is 15%₃)₃Solution, draws the 5mL Gd (NO of preparation₃)₃Solution, 10mLNa₂WO₄Molten Liquid and 5mL Eu (NO₃)₃Solution, joins in reactor, with dust technology, sodium hydroxide regulation pH=5, stirs, closes anti- Answer still, after 200 DEG C of heated at constant temperature 24h, be naturally down to room temperature；Solution in reactor is poured out standing, treats powder body precipitation in solution After, remove supernatant, with distilled water wash powder body 3 times, stand 3h every time；Washed powder body is heated to 80 DEG C of dry 12h, To NaGd (WO₄)₂: Eu³⁺Nano-particle；

(2) labelling of ZEN antibody

ZEN monoclonal antibody or Anti-TNF-α liquid solution 10000r/min are centrifuged 30min, remove impurity；Molten with deionized water Solve NaGd (WO₄)₂: Eu³⁺Nano-particle, is configured to the NaGd (WO that concentration is 0.1mg/mL₄)₂: Eu³⁺Nanoparticles solution, then Use 0.1mol/L K₂CO₃Solution regulation NaGd (WO₄)₂: Eu³⁺Nanoparticles solution is to pH 8.2；

Take 10mL NaGd (WO₄)₂: Eu³⁺Nanoparticles solution, under stirring, adds ZEN monoclonal antibody or Anti-TNF-α Liquid solution 100 μ l, ZEN monoclonal antibody or polyclonal antibody solution concentration 1mg/mL, mixing, incubation at room temperature 30min, 4 DEG C, 10000r/min is centrifuged 30min, abandons supernatant, and precipitation is used 0.02mol/L Na₂B₄O₇Solution is diluted to 10mL, 10000r/min Centrifugal 30min, precipitation uses 0.02mol/L Na₂B₄O₇Solution is diluted to 1mL, and 4 DEG C save backup.