CN106053851A - Prealbumin detection kit with high stability - Google Patents
Prealbumin detection kit with high stability Download PDFInfo
- Publication number
- CN106053851A CN106053851A CN201610643014.4A CN201610643014A CN106053851A CN 106053851 A CN106053851 A CN 106053851A CN 201610643014 A CN201610643014 A CN 201610643014A CN 106053851 A CN106053851 A CN 106053851A
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- China
- Prior art keywords
- reagent
- prealbumin
- kit
- stability
- stabilizer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
The invention provides a prealbumin detection kit with high stability. The prealbumin detection kit comprises a R1 reagent and a R2 reagent, wherein the R2 reagent comprises the following components: a buffer, sodium chloride, a preservative, a PA antibody and a stabilizer. Compared with the prior art, the invention has the following beneficial effects: 1, A special stabilizer, choline chloride, is added into the R2 reagent. The stabilizer can change configuration of the PA antibody so as to achieve the effect of stabilizing the reagent; and though having changed the configuration of the antibody, the stabilizer will not affect titer of the antibody; and 2, tests show that the detection kit has high accuracy and good precision if applied for detection and has excellent stability and long preservation time. The kit has a wide range of application, is convenient to promote and use, and can be applied to different levels of hospitals, health prevention departments and biomedical research and development institutions to measure content of prealbumin in human serum or blood plasma samples.
Description
Technical field
The present invention relates to the kit for testing prealbumin that a kind of stability is high, belong to clinical vitro detection reagent technique neck
Territory.
Background technology
Prealbumin, also known as transthyretin, is called for short PA, hepatocyte synthesizes, often show in electrophoretic separation
Albuminous front, about 12 hours its half-life.Measure prealbumin concentration in blood plasma clinically for understanding protein
Malnutrition, hepatic insufficiency, the albumin of ratio and transferrins there is higher sensitivity, understand what prealbumin is
The meaning, it is understood that the higher main cause of prealbumin is to be caused by pachyemia.Additionally also has a kind of generation being probably liver
Ability of thanking the most also is the reason causing prealbumin higher.Understanding prealbumin is any meaning, it is understood that in hepatic disease
It is on the low side that hepatocarcinoma, liver cirrhosis, chronic active hepatitis, obstructive jaundice etc. may appear as prealbumin.Generally, front
Albumin, transaminase, bilirubin all increase, and belong to acute hepatic disease more, especially in liver cancer patient and Patients With Obstructive Jaundice,
If prealbumin persistently reduces, illustrate that the state of an illness is continuous worsening.
PA, in addition to as the material of tissue repairing, can also be regarded as a kind of transporter, and it combines T4 and T3, and to T3 parent
Bigger with power, PA forms complex with retinol binding protein, has the effect of delivery vitamin A.Except sensitive as one
Nutrient protein index, PA when acute inflammation, malignant tumor, liver cirrhosis or nephritis its blood concentration decline.Specifically, front
Albuminous content is as follows with associating of disease:
1) during hepatic disease, prealbumin is more sensitive, it is believed that there is the front white egg of the 30% normal hepatopath of albumin
White minimizing, postnecrotic cirrhosis is almost zero.Cirrhosis, liver necrocytosis is relatively light, and prealbumin change is little, and prognosis is preferable, when
When the state of an illness is improved, prealbumin raises the most rapidly；
2) subacute severe hepatitis prealbumin is always at low value, therefore prealbumin can be used as judging hepatopathy prognostic indicator.Liver
Cancer and Patients With Obstructive Jaundice all can reduce, and its reduction degree and the state of an illness have substantial connection；
3) combine transaminase, bilirubin detection has discriminating meaning to dissimilar hepatic disease and non-hepatic disease, as front
Albumin, transaminase, bilirubin all increase, and belong to acute hepatic disease more, as prealbumin does not increases, and only transaminase, bilirubin
Increase, be considered as the disease of non-liver own；
4) nephrotic syndrome prealbumin does not reduces, and can also raise when diet is abundant.Malnutrition is born
During nitrogen balance, prealbumin reduces.
Kit for testing prealbumin less stable in the market, reduces use value clinically.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide the prealbumin detectable that a kind of stability is high
Box.The present invention reaches the effect of stable reagent by adding a kind of special stabilizer in R2 reagent, have developed one
Plant the kit for testing prealbumin that stability is high.
The present invention is achieved by the following technical solutions:
The present invention provides the kit for testing prealbumin that a kind of stability is high, and it includes R1 reagent and R2 reagent, wherein,
Described R2 reagent comprises by the following component of densitometer:
Preferably, described stabilizer is beta-schardinger dextrin-, choline chloride or mannitol.
Preferably, described stabilizer is choline chloride.
Preferably, described R1 reagent comprises the following component by concentration or percent by weight:
Preferably, the pH value of described buffer is 7.0～7.4.
Preferably, described buffer is trishydroxymethylaminomethane, propane sulfonic acid or 4-hydroxyethyl piperazine ethanesulfonic acid.
Preferably, described buffer is propane sulfonic acid.
Preferably, described preservative is Proclin 300.
Preferably, described surfactant is Tween 80, polysorbas20 or triton x-100.
Preferably, it is characterised in that described surfactant is tween 20.
Compared with prior art, the present invention has a following beneficial effect:
1, adding a kind of special stabilizer choline chloride in R2 reagent of the present invention, this stabilizer can be by changing
The configuration of PA antibody, reaches the effect of stable reagent, although this stabilizer changes the configuration of antibody simultaneously, but does not affects
The titer of this antibody；
2, test shows, applies test kit accuracy in detection of the present invention high, and precision is good, and test kit of the present invention
Good stability, the holding time is long；
3, the test kit of the present invention is applied widely, it is simple to promote the use of, and can be applicable to situation of all-level hospitals, sanitary precaution department
Content with the prealbumin in medical biotechnology R&D institution mensuration human serum or plasma sample.
Accompanying drawing explanation
By the detailed description non-limiting example made with reference to the following drawings of reading, the further feature of the present invention,
Purpose and advantage will become more apparent upon:
Fig. 1 is the standard curve of PA reference standard；
Fig. 2 is the experimental result of the present invention and the stability checking detection of Kang Hua biotinylation kit；
Human serum is carried out by each autoregressive parameter by Fig. 3 for being respectively adopted reagent of the present invention and PA reagent of the prior art simultaneously
Measure, measured value is carried out the schematic diagram of correlation analysis.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.Following example will assist in the technology of this area
Personnel are further appreciated by the present invention, but limit the present invention the most in any form.It should be pointed out that, the ordinary skill to this area
For personnel, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement.These broadly fall into the present invention
Protection domain.
Embodiment 1
Measure test kit composition as follows:
1) the consisting of of reagent R1:
2) the consisting of of reagent R2:
Various compositions to add successively under room temperature, or can add simultaneously, or individually packs and in detection
Before the most immediately prepare.Reagent detection ratio is 3:1.
The PA detection kit that the present embodiment describes, it is adaptable to various types of full automatic biochemical apparatus is complete with Hitachi 7080
As a example by automatic biochemical analyzer, its operation is such as table 1.Analysis method: Two point end assay, the i.e. consumption of reagent R1, R2 be respectively 225ul and
75ul, sample size 6ul；225ul reagent R1 adds 6ul sample and hatches 3～5min in 37 DEG C, reads absorbance A 0, adds 75ul
R2, hatches 5min for 37 DEG C, reads absorbance A 1, calculate Δ A=A1-A0；Detection dominant wavelength is 340nm.
Use this reagent and said determination method, the curve of the PA standard substance that employing Hitachi 7080 biochemistry analyzer records
(as shown in Figure 1), wherein X-axis represents PA content (mg/L)；Y-axis represents absorbance.
Table 1
Embodiment 2
Measure test kit composition as follows:
1) the consisting of of reagent R1:
2) the consisting of of reagent R2:
Various compositions to add successively under room temperature, or can add simultaneously, or individually packs and in detection
Before the most immediately prepare.Reagent detection ratio is 3:1.
The PA detection kit that the present embodiment describes, it is adaptable to various types of full automatic biochemical apparatus is complete with Hitachi 7080
As a example by automatic biochemical analyzer, its operation is such as table 1.Analysis method: Two point end assay, the i.e. consumption of reagent R1, R2 be respectively 225ul and
75ul, sample size 6ul；225ul reagent R1 adds 6ul sample and hatches 3～5min in 37 DEG C, reads absorbance A 0, adds 75ul
R2, hatches 5min for 37 DEG C, reads absorbance A 1, calculate Δ A=A1-A0；Detection dominant wavelength is 340nm.
Embodiment 3
Measure test kit composition as follows:
1) the consisting of of reagent R1:
2) the consisting of of reagent R2:
Various compositions to add successively under room temperature, or can add simultaneously, or individually packs and in detection
Before the most immediately prepare.Reagent detection ratio is 3:1.
The PA detection kit that the present embodiment describes, it is adaptable to various types of full automatic biochemical apparatus is complete with Hitachi 7080
As a example by automatic biochemical analyzer, its operation is such as table 1.Analysis method: Two point end assay, the i.e. consumption of reagent R1, R2 be respectively 225ul and
75ul, sample size 6ul；225ul reagent R1 adds 6ul sample and hatches 3～5min in 37 DEG C, reads absorbance A 0, adds 75ul
R2, hatches 5min for 37 DEG C, reads absorbance A 1, calculate Δ A=A1-A0；Detection dominant wavelength is 340nm.
Embodiment 4: stability experiment
This experiment purpose is detectable stability.
Operating procedure: utilize Hitachi 7080 automatic clinical chemistry analyzer, detection reality of different time under 2～8 DEG C of environment
Execute the blank absorbency of reagent described in example 1, compare the change of its absorbance.Use pure water as dummy in biochemistry analyzer
Detection, as requested record detection sample absorbance.Such as sample blank absorbency then under test dominant wavelength, grapher is tested
The absorbance of (end of this item setup parameter is a bit) at the end of reading.
Table 2 result shows, compared with Kang Hua biological reagent, in reagent of the present invention half a year, blank absorbency change is significantly less than
The blank absorbency of Kang Hua biological reagent, shows that reagent stability of the present invention is better than Kang Hua biological reagent.Fig. 2 is the present invention
Reagent tests the comparison diagram of blank absorbency with the checking of the Kang Hua biological reagent stability of 180 days.
It is respectively adopted the PA reagent of reagent of the present invention and Beijing Strong Biotechnologies, Inc., uses Hitachi complete certainly
50 parts of human serums (comprising normal and monstrosity) are measured by Automatic Biochemical Analyzer by each autoregressive parameter simultaneously, enter measured value
Row correlation analysis；Fig. 3 is analysis result, and what wherein X-axis represented is patients serum's result of reagent of the present invention mensuration, and Y-axis represents
Be patients serum's result of measuring of Beijing Strong Biotechnologies, Inc. reagent, coefficient R 2=0.9992, return
Returning equation is y=1.0191x-1.1458.
Table 2
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformation or amendment within the scope of the claims, this not shadow
Ring the flesh and blood of the present invention.
Claims (10)
1. the kit for testing prealbumin that a stability is high, it is characterised in that include R1 reagent and R2 reagent, wherein, institute
State R2 reagent and comprise by the following component of densitometer:
2. the kit for testing prealbumin that stability as claimed in claim 1 is high, it is characterised in that described stabilizer be β-
Cyclodextrin, choline chloride or mannitol.
3. the kit for testing prealbumin that stability as claimed in claim 2 is high, it is characterised in that described stabilizer is chlorine
Change choline.
4. the kit for testing prealbumin that stability as claimed in claim 1 is high, it is characterised in that described R1 reagent comprises
By concentration or the following component of percent by weight:
5. the kit for testing prealbumin that stability as described in claim 1 or 4 is high, it is characterised in that described buffer
PH value be 7.0～7.4.
6. the kit for testing prealbumin that stability as described in claim 1 or 4 is high, it is characterised in that described buffer
For trishydroxymethylaminomethane, propane sulfonic acid or 4-hydroxyethyl piperazine ethanesulfonic acid.
7. the kit for testing prealbumin that stability as claimed in claim 6 is high, it is characterised in that described buffer is third
Sulfonic acid.
8. the kit for testing prealbumin that stability as described in claim 1 or 4 is high, it is characterised in that described preservative
For Proclin 300.
9. the kit for testing prealbumin that stability as described in claim 1 or 4 is high, it is characterised in that live in described surface
Property agent is Tween 80, polysorbas20 or triton x-100.
10. the kit for testing prealbumin that stability as described in claim 1 or 4 is high, it is characterised in that its feature exists
In, described surfactant is tween 20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610643014.4A CN106053851A (en) | 2016-08-08 | 2016-08-08 | Prealbumin detection kit with high stability |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610643014.4A CN106053851A (en) | 2016-08-08 | 2016-08-08 | Prealbumin detection kit with high stability |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108535491A (en) * | 2018-03-22 | 2018-09-14 | 北京九强生物技术股份有限公司 | A kind of latex enhancing immune of Troponin I is than turbid detection kit |
| CN110531086A (en) * | 2019-08-30 | 2019-12-03 | 北京利德曼生化股份有限公司 | A kind of magnetic microparticle chemiluminescence kit and preparation method thereof measuring human body prealbumin content |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108535491A (en) * | 2018-03-22 | 2018-09-14 | 北京九强生物技术股份有限公司 | A kind of latex enhancing immune of Troponin I is than turbid detection kit |
| CN110531086A (en) * | 2019-08-30 | 2019-12-03 | 北京利德曼生化股份有限公司 | A kind of magnetic microparticle chemiluminescence kit and preparation method thereof measuring human body prealbumin content |
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Application publication date: 20161026 |