CN106053451A - Gold nano particle with modified surface - Google Patents
Gold nano particle with modified surface Download PDFInfo
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- CN106053451A CN106053451A CN201610341373.4A CN201610341373A CN106053451A CN 106053451 A CN106053451 A CN 106053451A CN 201610341373 A CN201610341373 A CN 201610341373A CN 106053451 A CN106053451 A CN 106053451A
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- gold nano
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- kreatinin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
- G01N2021/3155—Measuring in two spectral ranges, e.g. UV and visible
Abstract
The invention provides a gold nano particle with a modified surface. The provided gold nano particle is modified by adenosine/silver ions. After a gold nano solution is subjected to adenosine/silver ion surface modification, the gold nano solution is still dispersed, the color of the gold nano solution is wine red; the absorption peak of the gold nano solution appears at 520 nm; creatinine can carry out synergetic coordinated complexing with modified gold nano particles so as to crosslink and agglomerate gold nano particles; after complexing, the absorption at 520 nm is reduced, the absorption at 630 nm is increased, the color of the solution turns blue, and moreover, a quantitative relationship exists between the ratio of two absorption peaks and the concentration of creatinine. The provided gold nano particle can rapidly, sensitively, and conveniently measure the content of creatinine in an in-vitro biological sample, and thus an important reference is provided for the early stage diagnosis of kidney diseases and evaluation on kidney functions. Moreover, the provided method for preparing the gold nano particle has the advantages that the cost is low, the gold nano particle is easy to synthesize, and the preparation method can be applied to industrial production easily.
Description
Technical field
The present invention relates to metal nanoparticle Material Field, particularly relate to the golden nanometer particle of a class surface modification
(AuNPs) application and in kreatinin identification is measured, belongs to bio-molecular diagnostics field.
Background technology
Kidney disease has become the most universal.In recent years, the whole world tens people is suffering from kidney in various degree
Damage, thousands of patient dies from chronic nephropathy.Therefore, the early diagnosis particular importance of kidney disease.
Kreatinin (Creatinine), also known as creatinine, is the organic nitrogen-contg. compound that one molecular weight (113.1188) is less
Thing, is the metabolic end product of creatine and phosphagen, and its structural formula is as follows.
Kreatinin is the material that muscular tissue metabolism produces, and it is only drained by glomerule, not by reabsorption, and
And creatinine is small-molecule substance, be not combined with plasma protein.So when kidney impaired mechanical, the normal excretion of creatinine is hindered
Hindering, cause creatinine in serum content to increase, meanwhile, when renal dysfunction is not serious, in urine, the excretion of creatinine increases.Cause
This, in serum and urine, the mensuration of creatine concentration is extremely important.Urine creatine measures with serum creatinine simultaneously, can be used for calculating creatinine
Clearance rate, with the filtering function of auxiliary evaluation glomerule.The serum of normal Healthy People and urine creatinine level be about 50～
140 μMs and 3.5～34.6nM, deviate these scopes and imply that kidney may have occurred pathological changes.
The method of detection kreatinin has isotope dilution mass spectrometry, Raman scattering method, high performance liquid chromatography (HPLC), alkalescence at present
Picric acid method etc., but all it is not suitable for the analysis of the clinical isolated preparation of high-volume, or poor selectivity, or need special setting
Standby, routine clinical use is more difficult, is not extensively application.It is thus desirable to new efficient, sensitive, method efficiently.
Summary of the invention
It is an object of the invention to provide the golden nanometer particle system for kreatinin identification and application process thereof, with quickly,
Simply, the kreatinin in the biological specimen such as urine and serum is detected delicately.
It is desirable to provide golden nanometer particle of a kind of surface modification and preparation method thereof, the Jenner that described surface is modified
Rice corpuscles is the golden nanometer particle modified by adenosine/silver ion.Its preparation method includes that golden nanometer particle is carried out surface repaiies
The step of decorations, i.e. golden nanometer particle successively contact with adenosine and silver ion in a solvent.
The gold nano solution after adenosine/silver-ion topical is modified of the present invention still keeps dispersity, presents claret,
Absworption peak is had at 520nm；Kreatinin can occur collaborative ligand complex effect with the golden nanometer particle after modifying so that it is crosslinking is poly-
Collection, shows as absorbing at 520nm declining, and absorbing at 630nm increases, and solution colour becomes blue, and the ratio of absworption peak at two
Quantitative relationship is had with the concentration of kreatinin.Containing of kreatinin in vitro biological sample can be detected quick, sensitive, quickly and easily
Amount, provides important reference frame for the evaluation of renal function and the early diagnosis of kidney disease.Additionally, it is provided by the present invention
The method low cost preparing the said goods, be easily-synthesized, it is easy to accomplish industrialized production.
Based on this, the present invention further provides the golden nanometer particle modified on described surface in kreatinin identification and detection
Application.
Another further aspect, the present invention also provides for a kind of for kreatinin identification with the compositions of detection, contains in described compositions
There is the golden nanometer particle that above-mentioned surface is modified.
Accompanying drawing explanation
Accompanying drawing 6 width of the present invention:
The uv absorption spectra of (solid line) (dotted line) afterwards before Fig. 1 gold nano system addition kreatinin
The transmission electron microscope picture of (left) (right) afterwards before Fig. 2 gold nano system addition kreatinin
In Fig. 3 aqueous sample, ultra-violet absorption spectrum is with the variation diagram of Concentrations
In Fig. 4 aqueous sample, ultra-violet absorption spectrum absorption value A630/520nmLinear relationship with Concentrations
Fig. 5 simulates in urine sample, A630/520nmLinear relationship with Concentrations
In Fig. 6 Ox blood serum sample, A630/520nmLinear relationship with Concentrations
Detailed description of the invention
Without specified otherwise, the present invention is prepared the glass apparatus that product used and embathes post-drying by chloroazotic acid washing liquid the most in advance
Standby.
The present invention first consists in and discloses the golden nanometer particle that a kind of surface is modified, and on the basis of existing technology, it is the most main
Want the golden nanometer particle being characterised as being modified by adenosine/silver ion.
Further, the present invention provides the preparation method of the golden nanometer particle that above-mentioned surface modifies, including to Jenner's grain of rice
Son carries out the step of surface modification, is that golden nanometer particle successively contacts with adenosine and silver ion in a solvent.
In detailed description of the invention, carry out in the system that the assembling of golden nanometer particle is used, described adenosine and silver ion
Concentration be respectively 1～7 μM and 1～9 μM.
In detailed description of the invention, described golden nanometer particle concentration in assembly system is 2～12nM.Preferably 2.7～
10.8nM。
More specifically, the preparation method of the golden nanometer particle that the surface described in the invention described above is modified includes walking as follows
Rapid:
(1) solution of gold nanoparticles that concentration is 2～12nM is prepared；Preferably 2.7～10.8nM；
Heretofore described and the preparation method of golden nanometer particle have been described in the prior art, people in the art
The golden nanometer particle that member by using for reference or with reference to the way of prior art, can obtain size tunable in conjunction with the test of limited number of time is molten
Liquid；In the present invention, the particle diameter of described golden nanometer particle is 10～150nm；Preferably 10～20nm；
(2) adding adenosine in the solution of step (1), making adenosine concentration in system is 1～7 μM, after being sufficiently mixed
Centrifugal, abandon supernatant；
(3) precipitate of step (2) gained is dispersed in water (pH is 5～9) or (10mM's) PBS buffer solution, so
Rear addition AgNO3Aqueous solution, makes Ag+Concentration in system is 1～9 μM, is sufficiently mixed.
On the other hand, it is readily appreciated that, the golden nanometer particle that surface of the present invention is modified, also include by above-mentioned any system
The golden nanometer particle that surface obtained by Preparation Method is modified.
The present invention further provides a kind of for kreatinin identification with the compositions of detection, wherein contain above-mentioned any technology
The golden nanometer particle that surface described by scheme is modified.
Another further aspect, the present invention provides golden nanometer particle the answering in kreatinin identification and detection that described surface is modified
With.
Application detailed description of the invention, be simulation urine and Ox blood serum in kreatinin identification and detection in application.Its
In, when being used for simulating kreatinin identification and detection in urine, the optimum system condition that assembles includes: the golden nanometer particle that surface is modified
In solution, adenosine concentration is 5 μMs, Ag+Concentration is 5 μMs, and golden nanometer particle concentration is 5.4nM, and detection Concentrations scope is
3.5～34.6nM.In Ox blood serum when kreatinin identification and detection, the optimum system condition that assembles includes: the gold that surface is modified
In nano-particle solution, adenosine concentration is 5 μMs, Ag+Concentration is 5 μMs, and golden nanometer particle concentration is 5.4nM, detects Concentrations
Scope is 50～140 μMs.
Certainly, in different application systems, the system assembling condition of the golden nanometer particle that above-mentioned surface is modified and detection
Application conditions is different.Those skilled in the art passes through description of the invention, and combines the enlightenment of prior art, completely may be used
To implement.
Being described further present disclosure below in conjunction with non-limiting example, these embodiments should not be managed
Solve as restriction any form of to present invention.
The preparation of embodiment 1. golden nanometer particle (AuNPs) solution
Material/agent: gold chloride (HAuCl4·3H2And sodium citrate (Na O)3C6H5O7·2H2O)。
Method: with reference to Controlled Nucleation for the Regulation of the Particle
Size (method (PHYSICAL described in NATURE in Monodisperse Gold Suspensions mono-literary composition
SCIENCE, volume 241, on January 1st, 1973): with water as solvent, preparation mass concentration is the HAuCl of 0.01%4Solution and 1%
Sodium citrate solution.By the above-mentioned HAuCl of 50mL4After solution is heated to boiling, add the sodium citrate solution of different amounts.
After about 25 seconds, the solution of boiling becomes light blue, and after about 70 seconds, blue solution quickly becomes shiny red, and reaction completes.
Use this preparation method, by adjusting the consumption of sodium citrate solution to control different sodium citrate/gold chlorides
Ratio, can prepare containing 12～150nm particle diameters, favorable dispersibility, the AuNPs solution of uniform particle sizes.
The AuNPs solution containing 13nm prepared by the selection of the present invention following embodiment.
Embodiment 2. is for modifying the surface functional group screening test of AuNPs
In the present embodiment, the functional molecular participated in the experiment is: L-Histidine, GSH, Cys, adenosine, carbamide,
Folic acid, glycolylurea, uric acid, thymus pyrimidine, tyrosine, phenylboric acid, uracil and anhydrous creatine.The most prewired for aqueous solution.
In the present embodiment, the slaine participated in the experiment is: BaCl2,HgCl2·0.5H2O,CoCl2,Ni(NO3)2·6H2O,Cr
(NO3)3,Pb(NO3)2,FeCl3·6H2O,Zn(NO3)2·6H2O,Cu(CH3COO)2·H2O,AgNO3,Mn(CH3COO)2·
4H2O,AlCl3.The most prewired for aqueous solution.
The present embodiment uses according to the AuNPs solution containing 13nm obtained by the method for embodiment 1.
Method:
(1) in the obtained AuNPs solution 500 μ L containing 13nm of embodiment 1, being initially charged prewired concentration is 0.5mM's
Functional molecular aqueous solution, making the concentration of functional molecular in system is 5 μMs, centrifugal after being sufficiently mixed, and abandons sinking gained after supernatant
Shallow lake thing is dispersed in water, is subsequently adding the aqueous metal salt that prewired concentration is 0.5mM, makes the concentration of metal ions in system
It is 5 μMs, mix homogeneously, it is thus achieved that functional molecular/metal ion-AuNPs system.
(2) adding concentration in the system prepared by step (1) is 0.5mM kreatinin solution, makes kreatinin in system dense
Degree is 5 μMs, observes phenomenon, if solution of gold nanoparticles is become blue from claret, then system has response to kreatinin, if Jenner
Rice corpuscles solution invariant color, then system to kreatinin without response.
Result: as shown in table 1, × expression system is to kreatinin without response, and √ represents that system has response to kreatinin；Can
Seeing: in the range of sizable screening, kreatinin is had by the AuNPs system that only adenosine/silver ion and uric acid/mercury ion are modified
Response, the AuNPs system of the little molecule/Metal Ions Modification of remaining function to kreatinin without response.And mercury ion has bigger
Toxicity.
Table 1
Embodiment 3. adenosines/silver ion-AuNPs system identification kreatinin test
Material/agent: kreatinin, the AuNPs solution obtained by embodiment 1
Method: the 1. assembling of the AuNPs system that adenosine/silver ion is modified
In the AuNPs solution of 500 μ L obtained by embodiment 1, it is initially charged the adenosine solution that concentration is 0.5mM of 5 μ L
After (in system, concentration is 5 μMs), fully concussion mixing, within 10 minutes, be centrifuged with 10000 turns, after Aspirate supernatant, then
It is added thereto to after the deionized water of 500 μ L makes its redispersion, add the AgNO that concentration is 0.5mM of 5 μ L3Solution is (in system
Concentration is 5 μMs), obtain adenosine/silver ion-AuNPs system after mixing.
2. the system test to kreatinin
The concentration adding 5 μ L in the adenosine/silver ion-AuNPs system of 500 μ L prepared by embodiment 3.1 is 0.5mM
Stand 5 minutes after kreatinin solution (concentration is 5 μMs), fully mixing, observe color change, and use ultraviolet-uisible spectrophotometer
(UV-vis 2501PC) surveys the absorption spectrum of the gold nano system before and after addition kreatinin.
Result: as shown in Figure 1.Before reacting with kreatinin (solid line), the adenosine of the present invention/silver ion-AuNPs system is
Claret liquid, absworption peak is at 520nm；Adding after kreatinin reaction (dotted line), system becomes blue, and 520nm absworption peak shows
Write and decline, notable rising at 630nm.
Embodiment 4. adenosines/silver ion-AuNPs assembly system Exploration of Mechanism test:
Material/agent: adenosine, silver nitrate, kreatinin
Method: to adenosine and silver ion, kreatinin and silver ion, the mixed liquor of adenosine, silver ion and kreatinin enters respectively
Row mass spectral analysis.Zeta current potential before and after assembling adenosine/silver ion-AuNPs system measures, overview function molecule and gold
Belong to whether ion is modified on golden nanometer particle.With transmission electron microscope to adenosine/silver ion-golden nanometer particle system and flesh
Before and after anhydride reaction, the dispersity of AuNPs has carried out sign directly perceived, observes its state change before and after adding kreatinin.
Result: the molecular ion peak of 1:1 and 2:1, kreatinin and silver occur in the mass spectrum of adenosine and silver ion mixed liquor
The mass spectrum of Ar ion mixing liquid occurs the molecular ion peak of 1:1, in the mass spectrum of adenosine, silver ion and kreatinin mixed liquor
The molecular ion peak of 1:1:1 there is, it was demonstrated that the collaborative complexing of three.The zeta current potential of AuNPs is-44mV, adds gland
After glycosides, silver ion, kreatinin, become-25.5mV ,-17.4mV ,-10.9mV successively, it was demonstrated that each molecule passes through with golden nanometer particle
Electrostatic interaction and assemble.Be can be observed by transmission electron microscope, after adding kreatinin, system is become significantly assembling from dispersity
State (accompanying drawing 2), it was demonstrated that the system response to kreatinin.
The optimization of embodiment 5. adenosines/silver ion-AuNPs system
Material/agent: adenosine, silver nitrate, kreatinin, golden nanometer particle
Method: by control variate method adenosine, silver ion, gold nano solution concentration, solution acid alkalinity and inspection to system
The conditions such as survey time are optimized.20 μMs are configured with deionized water, 60 μMs, 80 μMs, the kreatinin sample of 100 μMs and 140 μMs,
Under optimal conditions, respectively the sample of 5 μ L is joined in 500 μ L adenosines/silver ion-AuNPs system, observe color after reaction and become
Change, after 6min, measure ultraviolet absorption curve.
Result: by optimize optimal conditions is: [adenosine]=5 μM, [Ag+]=5 μM, [AuNPs]=5.4nM, solvent
For 10mM PBS, the testing time is 6min.The change of ultra-violet absorption spectrum is relevant with Concentrations (accompanying drawing 3), and
A630/520nmThe concentration of numerical value and kreatinin present linear relationship (accompanying drawing 4).Embodiment 6. adenosines/silver ion-AuNPs system
Kreatinin test in detection simulation urine
Material/agent: kreatinin, CaCl2、MgSO4、NaHCO3、Na2C2O4、Na2SO4、NaH2PO4、Na2HPO4、NaCl、
KCl、NH4Cl, carbamide, uric acid, kreatinin and sodium citrate, the adenosine/silver ion-AuNPs system of embodiment 3.2 preparation.
Method: urine environmental simulation: dissolve CaCl in 200mL deionized water2(0.089g)、MgSO4(0.100g)、
NaHCO3(0.034g)、Na2C2O4(0.003g)、Na2SO4(0.258g)、NaH2PO4(0.100g)、Na2HPO4(0.011g)、
NaCl(0.634g)、KCl(0.450g)、NH4Cl (0.161g), carbamide (2.427g), uric acid (0.034g), kreatinin
(0.090g) with sodium citrate (0.297g), if having, trace solid is undissolved can suitably be heated, or ultrasonic dissolution assisting.The simulation of preparation
Liquid, puts into Refrigerator store.
Take 10mL simulated solution, add different amounts of kreatinin, and diluted 200 times stand-by, prepared concentration is respectively 20 μ
M, 60 μMs, 100 μMs, the kreatinin test fluid of 160 μMs and 200 μMs.
At optimum assembling condition ([adenosine]=5 μM, [Ag+]=5 μM, [AuNPs]=5.4nM, solvent is 10mM PBS,
Testing time is 6min) under, the difference being separately added into above-mentioned preparation respectively in 500 μ L adenosines/silver ion-AuNPs system is dense
The each 5 μ L of kreatinin test fluid of degree, observe color change after reaction, measure ultraviolet-visible absorption spectroscopy, with A after 6min630/520nm
The concentration of numerical value and kreatinin set up working curve.Result: Concentrations and system A in simulation urine630/520nmNumerical value
As shown in Figure 5, its mark-on reclaims test result and see table 2 (parallel assays five times), wherein raw sample actual concentrations (C) relation
With recording concentration (c) relation it is: C(nM)=c(μM)*20。
Table 2: the mensuration of kreatinin content in simulation urine
| Sample | Addition (μM) | Record meansigma methods ± standard deviation (μM) | The response rate (%) |
| 1 | 0.8 | 1.02±0.042 | 127.5 |
| 2 | 1.8 | 1.66±0.13 | 92.2 |
Kreatinin test in embodiment 7. adenosines/silver ion-AuNPs system detection Ox blood serum
Material/agent: kreatinin, trichloroacetic acid, NaOH, the adenosine/silver ion-AuNPs system of embodiment 3.2 preparation.
Method: take 2ml Ox blood serum, be charged with 300g/L, the trichloroacetic acid of 150 μ L, first acutely vibrate 2min, then will
Mixture is centrifuged 10min with 10000r/min, takes supernatant liquid filtering, and being adjusted to pH with the NaOH of 1M is 7.0, then with 10000r/min
Centrifugal 10min, takes supernatant, and with the membrane filtration of 0.22 μm.It is made into 50 μMs with the treatment fluid obtained, 100 μMs, 150 μMs,
300 μMs and the sample of 400 μMs.At optimum assembling condition ([adenosine]=5 μM, [Ag+]=5 μM, [AuNPs]=5.4nM, molten
Agent is 10mM PBS, and the testing time is 6min) under, respectively the sample of 5 μ L is joined 500 μ L adenosines/silver ion-AuNPs body
In system, after reaction, observe color change, measure ultraviolet-visible absorption spectroscopy after 6min, with A630/520nmNumerical value and kreatinin
Concentration sets up working curve.
Result: Concentrations and system A in Ox blood serum630/520nmNumerical relation as shown in Figure 6, set up work bent
Line, its mark-on reclaims test result and see table 3 (parallel assays five times).
Table 3: the mensuration of kreatinin content in bovine serum albumin
| Sample | Addition (μM) | Record meansigma methods ± standard deviation (μM) | The response rate (%) |
| 1 | 125 | 110.4±17.04 | 88.3 |
| 2 | 200 | 245.0±15.86 | 122.5 |
Claims (9)
1. the golden nanometer particle that surface is modified, it is characterised in that be the golden nanometer particle modified by adenosine/silver ion.
2. the preparation method of the golden nanometer particle that the surface described in claim 1 is modified, carries out surface including to golden nanometer particle
The step modified, it is characterised in that be that golden nanometer particle successively contacts with adenosine and silver ion in a solvent.
Method the most according to claim 2, it is characterised in that described adenosine and the silver ion concentration in system is respectively
It it is 1～7 μM and 1～9 μM.
Method the most according to claim 2, it is characterised in that described golden nanometer particle concentration in system be 2～
12nM。
Method the most according to claim 2, it is characterised in that comprise the steps:
(1) solution of gold nanoparticles that concentration is 2～12nM is prepared；
(2) adding adenosine in the solution of step (1), making adenosine concentration in system is 1～7 μM, centrifugal after being sufficiently mixed,
Abandon supernatant；
(3) precipitate of step (2) gained is dispersed in water or PBS buffer solution, is subsequently adding AgNO3Aqueous solution, makes Ag+
Concentration in system is 1～9 μM, is sufficiently mixed.
Method the most according to claim 5, it is characterised in that in described step (1), solution of gold nanoparticles concentration is 2.7
～10.8nM.
Method the most according to claim 5, it is characterised in that in described step (1) particle diameter of golden nanometer particle be 10～
150nm。
8. for kreatinin identification and the compositions of detection, it is characterised in that the gold modified containing the surface described in claim 1
Nanoparticle.
9. the golden nanometer particle that the surface described in claim 1 is modified application in kreatinin identification and detection.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175675A (en) * | 2011-01-20 | 2011-09-07 | 福州大学 | Method for detecting copper ions |
| CN103852432A (en) * | 2014-03-24 | 2014-06-11 | 中南民族大学 | Method for detecting bisphenol A by p-mercaptophenol modified nano-gold |
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2016
- 2016-05-20 CN CN201610341373.4A patent/CN106053451A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175675A (en) * | 2011-01-20 | 2011-09-07 | 福州大学 | Method for detecting copper ions |
| CN103852432A (en) * | 2014-03-24 | 2014-06-11 | 中南民族大学 | Method for detecting bisphenol A by p-mercaptophenol modified nano-gold |
Non-Patent Citations (3)
| Title |
|---|
| JIANJUN DU等: "Colorimetric Detection of Creatinine Based on Plasmonic Nanoparticles via Synergistic Coordination Chemistry", 《SMALL》 * |
| XIAOPENG HUANG等: "Glutathione-Protected Hierarchical Colorimetric Response of Gold Nanoparticles: a Simple Assay for Creatinine Rapid Detection by Resonance Light Scattering Technique", 《PLASMONICS》 * |
| YI HE等: "Gold nanoparticles-based colorimetric and visual creatinine assay", 《MICROCHIM ACTA》 * |
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