CN106053447A - Method for measuring soil carbon sequestration effect in paddy rice growth period and biocarbon composition - Google Patents

Method for measuring soil carbon sequestration effect in paddy rice growth period and biocarbon composition Download PDF

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CN106053447A
CN106053447A CN201610312402.4A CN201610312402A CN106053447A CN 106053447 A CN106053447 A CN 106053447A CN 201610312402 A CN201610312402 A CN 201610312402A CN 106053447 A CN106053447 A CN 106053447A
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soil
charcoal
carbon sequestration
rice
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CN106053447B (en
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张玉虎
赵远
曹宝
侯国军
杨泽平
孙辰鹏
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Capital Normal University
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02—Superphosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention relates to a method for measuring the soil carbon sequestration effect in the paddy rice growth period. The method comprises the following steps: collecting soil to be detected before a test, simulating the environment after natural growth of paddy rice, collecting the soil to be detected, and measuring the organic carbon content of the soil, wherein the organic carbon content of soil is measured by a potassium dichromate-concentrated sulfuric acid oxidation method, the supernate is compared with standard color classes prepared from a standard solution, and the soil organic carbon content is calculated according to a formula: soil organic carbon percentage content= colorimetric reading number* correction coefficient. The measuring method is simple, the test results are accurate, and the research on the mechanism of soil carbon sequestration is greatly assisted by the method.

Description

The assay method of rice growing season soil carbon sequestration effect and a kind of charcoal compositions
Technical field
The invention belongs to carbon sequestration monitoring technical field, particularly to the assay method of rice growing season soil carbon sequestration effect.
Background technology
For natural potential and the main path of clear and definite soil carbon sequestration and reduction of greenhouse gas discharge, soil carbon sequestration Mechanism Study is Through becoming an extremely active emerging research neck of agricultural soil carbon circulating research in the research of reply climate change in recent years Territory.China's table soil average organic carbon density is only the 70%～75% of European Union's meansigma methods, and the national conditions of this low soil carbon density are The agrological carbon fixation and emission reduction of Science in Future in China provides huge space, is also development and the enhancing of sustainable agriculture production system The ability of reply climate change provides natural potential.
Rice soil, as the artificial anthropogenic soil formed under a kind of special Land use systems, has higher carbon density and bigger Carbon sequestration potentiality, its to alleviate Atmospheric CO2Rising and the reply climate change of concentration have considerable effect.Nearest grinds Studying carefully display, over nearly 20 years, Farmland in China soil organic matter shows a rising trend, and especially south rice soil Organic carbon accumulation is the most relatively Hurry up, and mineralising stability is higher, therefore, the agriculture carbon remittance effect under rice in China production structure adjustment is followed with carbon at Global Agriculture Having great importance in ring research, the assay method of current paddy soil carbon sequestration effect is the most complex, and measurement result is by each The impact of the factor of kind is bigger.
Summary of the invention
In order to solve above-mentioned technical problem, the present invention provides the assay method of a kind of rice growing season soil carbon sequestration effect, Technical scheme is:
The present invention provides the assay method of rice growing season soil carbon sequestration effect, comprises soil collecting method and soil is organic Determination of Carbon, it is characterised in that in described assay method, organic carbon content assay method is as follows:
Weigh 1-3g soil to be measured carry out oxidation air-dry put in test tube, add 30-50mL 0.167mol/L potassium dichromate Solution vibrates, then is rapidly added the concentrated sulphuric acid of 25-35mL mass fraction 95%, stands 40-50min, stand after vibration 3-5min Draw 5-10 with dropper afterwards and drip the supernatant, add in colorimetric porcelain plate hole, add 3-5 and drip distilled water, stir evenly, with different carbonaceous amounts The standard color range that the standard solution of concentration is made carries out colorimetric, writes down colorimetric readout, carries out the survey of soil organic matter percentage composition Fixed, described soil organic matter percentage composition=colorimetric readout × correction coefficient.
Further, described standard solution is glucose solution, and described standard color range is simulation color range, at 590nm wavelength The absorption photometric value gained of the lower glucose solution measuring different carbon mass concentration, after the supernatant being diluted, in 590nm wavelength Its absorption photometric value of lower survey, and the standard absorption photometric value corresponding with standard color range contrast.
Further, described soil is north sandy soil earth, and correction coefficient is 0.75；Described soil is soils in south china, correction Coefficient is 0.85；Wherein the north is the Qinling Mountains-Huaihe River one line with demarcation line, south.
Further, described soil collecting method comprises the steps:
(1) soil collecting to be measured before test:
Take field paddy growth and commonly use soil, be placed in cultivation tray, gather pedotheque by " diagonal " mode, select at random Take many basins soil to mix, obtain soil to be measured；
(2) simulated rice soil collecting to be measured after naturally growing:
Take field paddy growth and commonly use soil, be placed in cultivation tray, every basin adds soil described in 60-70g, in soil Watering and be uniformly mixed, the rice sprouts then choosing health is transplanted in cultivation tray, and every basin is uniformly planted 4-6 strain, transplants After pour water and manually turn over, other herbicides, insecticide and water management measure are identical with local management；After rice harves by " diagonal " mode gathers pedotheque, randomly selects many basins soil and mixes, obtains soil to be measured.
Preferably, the size of described cultivation tray is: diameter 45cm, high 30cm；Described soil collecting method gathers soil The width of sample is 1-40cm, and the degree of depth is 5-8cm.
Further, it is characterised in that the soil of described step (2) adds the charcoal of soil gross mass 10-15%, Preferably, described charcoal is that raw material is prepared from by waste and old bark and waste and old branch, and preparation method is as follows: taking mass ratio is 1: The waste and old bark of 40-1:3 and waste and old branch mixture, by its completely air-dry after pulverize, sieve to obtain 60-70 mesh powder, by powder Load in porcelain crucible, close the lid, be placed in the Muffle furnace of 580-680 DEG C being pyrolyzed 1.5-2.5h, after pyrolysis, be cooled to room temperature Taking out, by charcoal grinding after pyrolysis, sieve to obtain 200-220 mesh powder, is described charcoal.
On the other hand, present invention also offers a kind of charcoal compositions increasing rice growing season soil carbon sequestration capacity using, Raw material including following weight portion: 20-30 charcoal, 5-8 Flammulina velutiper (Fr.) Sing mushroom bran, 2-5 mannitol, 5-10 Gypsum Fibrosum powder, 1-5 polyoxy third Dilute glycerin ether, 4-9 double superhosphate, 10-15 Rhizoma Seu Herba Bergeniae, 10-13 flyash, 2-5 kieselguhr, 4-9 acrylic resin, described life It is that raw material is prepared from by waste and old bark and waste and old branch that thing charcoal is preferably above-mentioned.
Further, described compositions also includes the general bacterium of the agglomerate that mass ratio is 2:1:3:2 of 1-2 weight portion, solves starch Bacillus cereus, bacillus subtilis, the complex micro organism fungicide of button capsule plan endosporaceae, the soil carbon sequestration efficiency improved further.
Further, described charcoal compositions also includes that the mass ratio of 5-10 weight portion is the polyurethane of 1:1:2, west Tragacanth and the mixture of yeast rich in selenium, further improve the carbon sequestration efficiency of the soil adding described charcoal compositions, In particular for north sand.
On the other hand, present invention also offers the preparation method of described charcoal compositions, comprise the steps:
1) Flammulina velutiper (Fr.) Sing mushroom bran and Rhizoma Seu Herba Bergeniae are pulverized respectively, after mixing homogeneously with charcoal, flyash and kieselguhr, high pressure Sterilization treatment in autoclave, 130 DEG C, steaming and decocting 3 hours, after steaming and decocting, material is spread out, cool the fine powder that dries in the air stand-by；
2) by step 1) the fine powder water content of gained controls at 45%-50%, and add the general bacterium of agglomerate, solve starch spore bar Bacterium, bacillus subtilis, button capsule intend the complex micro organism fungicide of endosporaceae, with anaerobic fermentation in fermentation tank 15-20 days, fermentation temperature Degree, for 25-30 DEG C, obtains anaerobic fermented liquid；
3) by step 2) anaerobic fermented liquid that obtains and mannitol, Gypsum Fibrosum powder, polyoxy propylene glycerin ether, double superhosphate and Acrylic resin mix homogeneously, piles up neatly formula in 50-70 DEG C of bar and is aged 20-40 days, and period turns over buttress 3-6 time, de-stacking after fermentation, dry in the sun, Obtain described charcoal compositions.
The assay method of rice growing season soil carbon sequestration effect provided by the present invention, method is simple, and test result is accurate, Research soil carbon sequestration mechanism there is the biggest contribution.
Use the charcoal compositions of increase rice growing season soil carbon sequestration capacity using provided by the present invention, can be effectively improved The carbon sequestration effect of paddy soil, in particular for north sandy soil, significantly improves Soil structure, increases the product of soil agreegate Raw, but control aggregate size and the suitability of porosity, strengthen soil combination, holding and crystallized ability to nutrient, can subtract Few carbon release in air, and prevent the mineralising of soil organic matter, form the inertia carbon storehouse of soil, beneficially soil organic matter Accumulation.Additionally apply charcoal compositions of the present invention, organic fertilizer can be regarded, there is abundant organic matter and inorganic Nutrient, the growth to Oryza sativa L. has facilitation, is greatly improved yield and the quality of Oryza sativa L..
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
There is provided the assay method of rice growing season soil carbon sequestration effect, following steps:
(1) sandy loam that soil is North Japonica Rice experiment, the soil before gathering Rice Cropping respectively and after plantation are measured Measure；
(2) weigh 1g soil to be measured carry out oxidation air-dry put in test tube, add 30mL 0.167mol/L potassium dichromate molten Liquid vibrates, then is rapidly added the concentrated sulphuric acid of 25mL mass fraction 95%, stands 40min, inhale with dropper after standing after vibration 3min Take 5 supernatantes, add in colorimetric porcelain plate hole, add 3 distilled water, stir evenly, with the standard solution of different carbon mass concentrations The standard color range made carries out colorimetric, writes down colorimetric readout, carries out the mensuration of soil organic matter percentage composition, and described soil is organic Carbon percentage composition=colorimetric readout × correction coefficient, wherein correction coefficient is 0.75, and soil carbon capacity can reach 2.1-after measured 2.4t·hm-2·a-1
Embodiment 2
There is provided the assay method of rice growing season soil carbon sequestration effect, following steps:
(1) soil collecting to be measured before test:
Take the sandy loam of field North Japonica Rice experiment, be placed in cultivation tray, gather pedotheque by " diagonal " mode, Randomly select 8 basin soil to mix, obtain soil to be measured；
(2) simulated rice soil collecting to be measured after naturally growing:
Take the sandy loam of North Japonica Rice experiment, be placed in a diameter of 45cm of basin mouth, in the cultivation tray of high 30cm, every basin add Enter soil described in 70g, be added thereto to the charcoal that gross mass 10% prepares for raw material with waste and old bark and waste and old branch, Mixing and stirring, waters in soil and is uniformly mixed, and the rice sprouts then choosing health is transplanted in cultivation tray, often Basin is uniformly planted 6 strains, pours water and manually turn over after transplanting, other herbicides, insecticide and water management measure and local management Identical；Gathering width by " diagonal " mode after rice harves is 1cm, and the degree of depth is the pedotheque of 8cm, randomly selects 6 basins Soil mixes, and obtains soil to be measured.
(3) mensuration of organic carbon content:
Weigh 3g soil to be measured carry out oxidation air-dry put in test tube, add 50mL 0.167mol/L potassium bichromate solution Vibration, then it is rapidly added the concentrated sulphuric acid of 35mL mass fraction 95%, stand 50min after vibration 5min, draw with dropper after standing 10 supernatantes, add in colorimetric porcelain plate hole, add 5 distilled water, stir evenly, with the standard solution system of different carbon mass concentrations The standard color range become carries out colorimetric, writes down colorimetric readout, carries out the mensuration of soil organic matter percentage composition, described soil organic matter Percentage composition=colorimetric readout × 0.75, soil carbon capacity can reach 5.8-7.5t hm after measured-2·a-1。
Embodiment 3
There is provided the assay method of rice growing season soil carbon sequestration effect, following steps:
(1) soil collecting to be measured before test:
Take the loam of Along The Coastal Plain of Zhejiang Province rice belt, be placed in cultivation tray, gather pedotheque by " diagonal " mode, with Machine is chosen 5 basin soil and is mixed, and obtains soil to be measured；
(2) simulated rice soil collecting to be measured after naturally growing:
Take the loam of wild Along The Coastal Plain of Zhejiang Province rice belt, be placed in a diameter of 45cm of basin mouth, in the cultivation tray of high 30cm, every basin Soil described in middle addition 60g, waters in soil and is uniformly mixed, and the rice sprouts then choosing health is transplanted to cultivation tray In, every basin is uniformly planted 6 strains, pours water and manually turn over after transplanting, and other herbicides, insecticide and water management measure are with local Manage identical；Gathering width by " diagonal " mode after rice harves is 40cm, and the degree of depth is the pedotheque of 5cm, selects at random Take 10 basin soil to mix, obtain soil to be measured.
(3) mensuration of organic carbon content:
Weigh 2g soil to be measured carry out oxidation air-dry put in test tube, add 40mL 0.167mol/L potassium bichromate solution Vibration, then it is rapidly added the concentrated sulphuric acid of 30mL mass fraction 95%, stand 45min after vibration 4min, after standing, draw 8 with dropper Drip the supernatant, add in colorimetric porcelain plate hole, add 4 distilled water, stir evenly, with the standard solution of different carbon mass concentrations is made Standard color range carry out colorimetric, write down colorimetric readout, carry out the mensuration of soil organic matter percentage composition, described soil organic matter hundred Dividing content=colorimetric readout × 0.85, soil carbon capacity can reach 4.8t hm after measured-2·a-1。
Embodiment 4
Thering is provided the charcoal compositions increasing rice growing season soil carbon sequestration capacity using, described compositions includes following weight portion Raw material: 25 charcoals, 6 Flammulina velutiper (Fr.) Sing mushroom brans, 3 mannitol, 8 Gypsum Fibrosum powders, 3 polyoxy propylene glycerin ethers, 8 double superhosphate, 13 rocks Chinese cabbage, 12 flyash, 4 kieselguhr, 8 acrylic resins.
Embodiment 5
Thering is provided the charcoal compositions increasing rice growing season soil carbon sequestration capacity using, described compositions includes following weight portion Raw material: 20 charcoals, 5 Flammulina velutiper (Fr.) Sing mushroom brans, 2 mannitol, 5 Gypsum Fibrosum powders, 1 polyoxy propylene glycerin ether, 4 double superhosphate, 10 rocks Chinese cabbage, 10 flyash, 2 kieselguhr, 4 acrylic resins, 1 complex micro organism fungicide；Wherein complex micro organism fungicide is mass ratio The mixture of endosporaceae intended by the general bacterium of agglomerate, bacillus amyloliquefaciens, bacillus subtilis and button capsule for 2:1:3:2.
Embodiment 6
Thering is provided the charcoal compositions increasing rice growing season soil carbon sequestration capacity using, described compositions includes following weight portion Raw material: charcoal 30, Flammulina velutiper (Fr.) Sing mushroom bran 8, mannitol 5, Gypsum Fibrosum powder 10, polyoxy propylene glycerin ether 5, double superhosphate 9, rock are white Dish 15, flyash 13, kieselguhr 5, acrylic resin 9, complex micro organism fungicide 2, polyurethane 2.5, tragacanth 2.5, selenium-rich Yeast 5, wherein complex micro organism fungicide be mass ratio be the general bacterium of agglomerate of 2:1:3:2, bacillus amyloliquefaciens, bacillus subtilis The mixture of endosporaceae intended by bacterium and button capsule.
Embodiment 7
Thering is provided the charcoal compositions increasing rice growing season soil carbon sequestration capacity using, described compositions includes following weight portion Raw material: 25 charcoals, 6 Flammulina velutiper (Fr.) Sing mushroom brans, 4 mannitol, 8 Gypsum Fibrosum powders, 2 polyoxy propylene glycerin ethers, 6 double superhosphate, 13 rocks Chinese cabbage, 12 flyash, 2 kieselguhr, 4 acrylic resins, 1 complex micro organism fungicide；Wherein complex micro organism fungicide is mass ratio The mixture of endosporaceae intended by the general bacterium of agglomerate, bacillus amyloliquefaciens, bacillus subtilis and button capsule for 2:1:3:2.
Preparation method:
1) take waste and old bark and waste and old branch mixture that mass ratio is 1:40, pulverize after it is air-dried completely, sieve 60 mesh powder, load powder in porcelain crucible, close the lid, and are placed in the Muffle furnace of 580 DEG C being pyrolyzed 2.5h, cool down after pyrolysis Taking out to room temperature, by charcoal grinding after pyrolysis, sieve to obtain 200 mesh powder, is described charcoal；
2) Flammulina velutiper (Fr.) Sing mushroom bran and Rhizoma Seu Herba Bergeniae are pulverized respectively, with step 1) charcoal of gained, flyash and kieselguhr mixes After closing uniformly, sterilization treatment in high-pressure sterilizing pot, 130 DEG C, steaming and decocting 3 hours, after steaming and decocting, material is spread out, dry in the air cool that fine powder is treated With；
3) by step 2) the fine powder water content of gained controls 48%, to add described complex micro organism fungicide, with fermentation Anaerobic fermentation 17 days in tank, fermentation temperature is 28 DEG C, obtains anaerobic fermented liquid；
4) by step 3) anaerobic fermented liquid that obtains and mannitol, Gypsum Fibrosum powder, polyoxy propylene glycerin ether, double superhosphate and Acrylic resin mix homogeneously, piles up neatly formula in 60 DEG C of bars and is aged 30 days, and period turns over buttress 5 times, de-stacking after fermentation, dry in the sun, obtains described Charcoal compositions.
Embodiment 8
Thering is provided the charcoal compositions increasing rice growing season soil carbon sequestration capacity using, described compositions includes following weight portion Raw material: charcoal 28, Flammulina velutiper (Fr.) Sing mushroom bran 7, mannitol 3, Gypsum Fibrosum powder 8, polyoxy propylene glycerin ether 2, double superhosphate 5, rock are white Dish 12, flyash 11, kieselguhr 3, acrylic resin 6, complex micro organism fungicide 2, polyurethane 2, tragacanth 2, yeast rich in selenium 4, wherein complex micro organism fungicide be mass ratio be the general bacterium of agglomerate of 2:1:3:2, bacillus amyloliquefaciens, bacillus subtilis and The mixture of endosporaceae intended by button capsule；
Preparation method:
1) take waste and old bark and waste and old branch mixture that mass ratio is 1:3, by its completely air-dry after pulverize, sieve 70 Mesh powder, loads powder in porcelain crucible, closes the lid, and is placed in the Muffle furnace of 680 DEG C being pyrolyzed 1.5h, is cooled to after pyrolysis Room temperature is taken out, and by charcoal grinding after pyrolysis, sieve to obtain 220 mesh powder, is described charcoal；
2) Flammulina velutiper (Fr.) Sing mushroom bran and Rhizoma Seu Herba Bergeniae are pulverized respectively, with step 1) charcoal of gained, flyash and kieselguhr mixes After closing uniformly, sterilization treatment in high-pressure sterilizing pot, 130 DEG C, steaming and decocting 3 hours, after steaming and decocting, material is spread out, dry in the air cool that fine powder is treated With；
3) by step 2) the fine powder water content of gained controls 45%, add described complex micro organism fungicide, polyurethane, Tragacanth and yeast rich in selenium, anaerobic fermentation 15 days in fermentation tank, fermentation temperature is 30 DEG C, obtains anaerobic fermented liquid；4) will Step 3) anaerobic fermented liquid that obtains mixes with mannitol, Gypsum Fibrosum powder, polyoxy propylene glycerin ether, double superhosphate and acrylic resin Closing uniformly, pile up neatly formula in 50 DEG C of bars and be aged 40 days, period turns over buttress 6 times, de-stacking after fermentation, and dry in the sun obtains described charcoal compositions.
Embodiment 9
Thering is provided the charcoal compositions increasing rice growing season soil carbon sequestration capacity using, described compositions includes following weight portion Raw material: charcoal 20, Flammulina velutiper (Fr.) Sing mushroom bran 5, mannitol 2, Gypsum Fibrosum powder 5, polyoxy propylene glycerin ether 1, double superhosphate 4, rock are white Dish 10, flyash 10, kieselguhr 2, acrylic resin 4, complex micro organism fungicide 1, polyurethane 1.25, tragacanth 1.25, richness The mixture 2.5 of selenium yeast, wherein complex micro organism fungicide is that mass ratio is the general bacterium of agglomerate of 2:1:3:2, solves starch spore bar The mixture of endosporaceae intended by bacterium, bacillus subtilis and button capsule；
Preparation method:
1) take waste and old bark and waste and old branch mixture that mass ratio is 1:20, pulverize after it is air-dried completely, sieve 65 mesh powder, load powder in porcelain crucible, close the lid, and are placed in the Muffle furnace of 600 DEG C being pyrolyzed 2h, are cooled to after pyrolysis Room temperature is taken out, and by charcoal grinding after pyrolysis, sieve to obtain 210 mesh powder, is described charcoal；
2) Flammulina velutiper (Fr.) Sing mushroom bran and Rhizoma Seu Herba Bergeniae are pulverized respectively, with step 1) charcoal of gained, flyash and kieselguhr mixes After closing uniformly, sterilization treatment in high-pressure sterilizing pot, 130 DEG C, steaming and decocting 3 hours, after steaming and decocting, material is spread out, dry in the air cool that fine powder is treated With；
3) by step 2) the fine powder water content of gained controls 50%, adds described complex micro organism fungicide, polyurethane, Tragacanth and yeast rich in selenium, anaerobic fermentation 20 days in fermentation tank, fermentation temperature is 25 DEG C, obtains anaerobic fermented liquid；
4) by step 3) anaerobic fermented liquid that obtains and mannitol, Gypsum Fibrosum powder, polyoxy propylene glycerin ether, double superhosphate and Acrylic resin mix homogeneously, piles up neatly formula in 70 DEG C of bars and is aged 20 days, and period turns over buttress 3 times, de-stacking after fermentation, dry in the sun, obtains described Charcoal compositions.
The different charcoal compositions impact on rice growing season soil carbon sequestration capacity using of test example 1
One, test method
1.1 sandy loams taking North Japonica Rice respectively and the soil of south rice, carry out soil according to the method for embodiment 2 The mensuration of earth organic carbon content, obtains Oryza sativa L. organic carbon content SOC (g kg-1), and calculate soil organic matter according to equation below Density DSOC,
DSOC=SOC × r × TH × 0.1 (t ha-1)
Wherein, r is topsoil soils unit weight (g cm-3), can obtain according to soil texture inquiry in " China's soil species will "；TH For soil depth (cm)；
And according to plantation year number calculating soil carbon sequestration efficiency: δ=(DSOCa-DSOC0)/n(t·ha-1·a-1)；Wherein DSOC0For initial soil Organic Carbon Density, DSOCaFor Soil Organic Carbon Density after n.
1.2 according to step 2) in the charcoal compositions that adds in every basin soil in simulated rice Natural growth process Difference, is divided into 5 test group, is respectively as follows: the test 1 adding the charcoal being prepared into by waste and old bark and waste and old branch mixture Group, adds the test 2 groups of the charcoal compositions that embodiment 4 is provided, the charcoal compositions that addition embodiment 5 is provided Test 3 groups, add the test 4 groups of the charcoal compositions that embodiment 6 is provided and add in China specially CN102775201B public The test 5 groups of " the carbon sequestration high-activity microorganism fertilizer " opened.After 1.3 Oryza sativa L. simulation natures grow 1 year, detect the north respectively The carbon sequestration effect of soil and soils in south china, the results are shown in Table 1；The soil that different charcoal compositions is added in detection simultaneously is raw to Oryza sativa L. Long impact, the monitoring percentage of earbearing tiller of Oryza sativa L., average spike length and yield, the results are shown in Table 2-3.
Two, result of the test
Table 1 adds different charcoal compositions rice-cultivating soil carbon sequestration efficiency (t ha after a year-1·a-1)
From above-mentioned result of the test, compared with the pure charcoal testing 1 group, test 2 groups of charcoals to test 4 groups Compositions can be effectively improved the carbon sequestration efficiency of paddy soil, and the effect wherein testing 3 groups is excellent compared with testing 2 groups, tests the raising of 4 groups The best results of soil carbon sequestration efficiency, in particular in north sand, is greatly improved soil carbon sequestration effect, increases soil The accumulation of organic carbon；With test compared with in the of 5 groups, be respectively provided with significance difference the opposite sex.
Table 2 soils in south china adds the growing state of the soil cultivation Oryza sativa L. of different charcoal compositions
Table 3 soils of north China adds the growing state of the soil cultivation Oryza sativa L. of different charcoal compositions
From above-mentioned result of the test, charcoal compositions provided by the present invention is not only able to improve consolidating of paddy soil Carbon effect, also can promote paddy growth, improves rice yield and quality；Wherein test 4 groups of Oryza sativa L. percentage of earbearing tiller, average spike length and product Measure the highest, be respectively provided with the significance difference opposite sex compared with testing 1 group and test 5 groups.

Claims (10)

1. an assay method for rice growing season soil carbon sequestration effect, comprises soil collecting method and soil organic carbon is surveyed Determine method, it is characterised in that in described assay method, soil organic carbon assay method is as follows:
Weigh 1-3g soil to be measured carry out oxidation air-dry put in test tube, add 30-50mL0.167mol/L potassium bichromate solution Vibration, then it is rapidly added the concentrated sulphuric acid of 25-35mL mass fraction 95%, stand 40-50min after vibration 3-5min, use after standing Dropper is drawn 5-10 and is dripped the supernatant, adds in colorimetric porcelain plate hole, adds 3-5 and drip distilled water, stir evenly, with different carbon mass concentrations The standard color range made of standard solution carry out colorimetric, write down colorimetric readout, carry out the mensuration of soil organic matter percentage composition, institute State soil organic matter percentage composition=colorimetric readout × correction coefficient.
2. the assay method of rice growing season soil carbon sequestration effect as claimed in claim 1, it is characterised in that described standard is molten Liquid is glucose solution, and described standard color range is simulation color range, measures the glucose of different carbon mass concentration under 590nm wavelength The absorption photometric value gained of solution.
3. the assay method of the solid charcoal effect of rice growing season soil as claimed in claim 2, it is characterised in that described soil is North sandy soil earth, correction coefficient is 0.75, or described soil is soils in south china, and correction coefficient is 0.85.
4. the assay method of rice growing season soil carbon sequestration effect as claimed in claim 1, it is characterised in that described soil is adopted Diversity method comprises the steps:
(1) soil collecting to be measured before test:
Take field paddy growth and commonly use soil, be placed in cultivation tray, gather pedotheque by " diagonal " mode, randomly select 5- 8 basin soil mix, and obtain soil to be measured；
(2) simulated rice soil collecting to be measured after naturally growing:
Take field paddy growth and commonly use soil, be placed in cultivation tray, every basin adds soil described in 60-70g, waters in soil And be uniformly mixed, the rice sprouts then choosing health is transplanted in cultivation tray, and every basin is uniformly planted 4-6 strain, fills after transplanting Water is also manually turned over, and other herbicides, insecticide and water management measure are identical with local management；By " right after rice harves Linea angulata " mode gathers pedotheque, and randomly select 5-10 basin soil and mix, obtain soil to be measured.
5. the assay method of rice growing season soil carbon sequestration effect as claimed in claim 4, it is characterised in that described cultivation tray Size be: diameter 45cm, high 30cm；The width gathering pedotheque in described soil collecting method is 1-4cm, and the degree of depth is 5- 8cm。
6. the assay method of rice growing season soil carbon sequestration effect as claimed in claim 4, it is characterised in that described step (2) add soil gross mass 10-15% in soil is the charcoal that raw material is prepared from by waste and old bark and waste and old branch.
7. the charcoal compositions increasing rice growing season soil carbon sequestration capacity using, it is characterised in that described compositions includes The raw material of following weight portion: 20-30 charcoal, 5-8 Flammulina velutiper (Fr.) Sing mushroom bran, 2-5 mannitol, 5-10 Gypsum Fibrosum powder, 1-5 polyoxy propylene are sweet Oil ether, 4-9 double superhosphate, 10-15 Rhizoma Seu Herba Bergeniae, 10-13 flyash, 2-5 kieselguhr, 4-9 acrylic resin.
8. increase the charcoal compositions of rice growing season soil carbon sequestration capacity using as claimed in claim 7, it is characterised in that described Compositions also include the general bacterium of the agglomerate that mass ratio is 2:1:3:2 of 1-2 weight portion, bacillus amyloliquefaciens, bacillus subtilis, The complex micro organism fungicide of endosporaceae intended by button capsule.
9. as described in claim 7 or 8, increase the charcoal compositions of rice growing season soil carbon sequestration capacity using, it is characterised in that Described charcoal compositions also includes that the mass ratio of 5-10 weight portion is the polyurethane of 1:1:2, tragacanth and yeast rich in selenium Mixture.
10. increase the charcoal compositions of rice growing season soil carbon sequestration capacity using as claimed in claim 8, it is characterised in that institute The preparation method stating compositions is as follows:
1) Flammulina velutiper (Fr.) Sing mushroom bran and Rhizoma Seu Herba Bergeniae are pulverized respectively, after mixing homogeneously with charcoal, flyash and kieselguhr, autoclaving Sterilization treatment in Guo, 130 DEG C, steaming and decocting 3 hours, after steaming and decocting, material is spread out, cool the fine powder that dries in the air stand-by；
2) by step 1) the fine powder water content of gained controls at 45%-50%, adds described complex micro organism fungicide, in fermentation Anaerobic fermentation 15-20 days in tank, fermentation temperature is 25-30 DEG C, obtains anaerobic fermented liquid；
3) by step 2) anaerobic fermented liquid that obtains and mannitol, Gypsum Fibrosum powder, polyoxy propylene glycerin ether, double superhosphate and propylene Acid resin mix homogeneously, piles up neatly formula in 50-70 DEG C of bar and is aged 20-40 days, and period turns over buttress 3-6 time, de-stacking after fermentation, and dry in the sun obtains Described charcoal compositions.
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CN107548649A (en) * 2017-08-17 2018-01-09 广西科技师范学院 A kind of foliage-spray geniposide promotes the method and its evaluation method of paddy growth
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