CN106047942A - Method for increasing anaerobic fermentation salt resistance and gas production performance of kitchen wastes - Google Patents
Method for increasing anaerobic fermentation salt resistance and gas production performance of kitchen wastes Download PDFInfo
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- CN106047942A CN106047942A CN201610589811.9A CN201610589811A CN106047942A CN 106047942 A CN106047942 A CN 106047942A CN 201610589811 A CN201610589811 A CN 201610589811A CN 106047942 A CN106047942 A CN 106047942A
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- anaerobic fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention provides a method for increasing anaerobic fermentation salt resistance and gas production performance of kitchen wastes by adding ectoine. The method is characterized in that the ectoine is added at the beginning of the anaerobic fermentation of the kitchen wastes, the microbial cell osmotic pressure constantly increasing due to salt accumulation is maintained, inhibition of salt to microbial activity is lowered, system salt tolerance is increased, and accordingly methane production rate is increased, reaction time is shortened, and the utilization rate of the kitchen wastes is increased. The method is simple to operate, and the added ectoine is low in cost and free of potential safety hazard.
Description
Technical field
The method that the present invention relates to anaerobic fermentation of kitchen waste, is specifically related to a kind of tetrahydropyrimidine that adds and improves changing food waste
Anaerobic fermentation salt resistance ability and the method for gas generation property.
Background technology
Changing food waste refers to residential quarter, business, the food garbage produced for source such as machine-operated and industrial, along with economical
Development and the raising of living standards of the people so that China's changing food waste generation amount presents the trend risen year by year, current year produces
Amount has reached more than 60,000,000 tons, as a example by Beijing, reaches every day more than 2000 ton the end of the year 2015.Have aqueous for changing food waste
The characteristics such as rate is high, content of organics is high, anaerobic fermentation is one of appropriate technology of changing food waste process, thus can not only eliminate
The environmental pollution brought, the most also realizes changing food waste into resources recycling.
Different from general anaerobic fermentation raw material such as human and animal excreta, straw etc., changing food waste salinity is high, in changing food waste
Inorganic salts includes calcium, magnesium, potassium, ferrum, sodium etc., and wherein the content of sodium salt is the highest.China's changing food waste salinity 2000～
6000mg/L(Na+Concentration) between, and constantly accumulate in continuous print anaerobic fermentation process Zhong Yan branch, this feature can be serious
The efficient stable affecting anaerobic fermentation of kitchen waste system runs, thus limits the application of anaerobic fermentation of kitchen waste.Document: Jiang
Refined. changing food waste Chinese anaerobic digestion process performance and feature analysis of Influential Factors [D]. Southeast China University's .2009. literary composition is studied
Claim, for the anaerobic fermentation of changing food waste, work as Na+When concentration is 3000mg/L, anaerobic fermentation of kitchen waste process is begun with
Inhibitory action, and Na+Concentration more high toxicity is the strongest.Document: Iacovidou E, Ohandja D G, Voulvoulis N.Food
waste disposal units in UK households:The need for policy intervention[J]
.Science of the Total Environment, in 2012,423 (8): 1 7. literary compositions, research claims, and detests for changing food waste
Aerobe fermentation, works as Na+Concentration more than 5000mg/L time, final methane can be converted and have a negative impact.
Why salinity is too high can produce inhibitory action to the growth of microorganism, main reason is that: salinity is too high
Time, cause Premeabilisation of cells pressure drastically to raise, hyperosmosis can make microbial cell dehydration cause cellular plasm to separate, high salt feelings
Dehydrogenase activity is made to reduce because of salting out under condition.
Summary of the invention
In the face of hyperosmosis environment, most of salt-durable microbes are by synthesizing and accumulation compatible solute in intracellular
(Compatible solutes) regulates the change of osmotic pressure and maintains the normal metabolic activity of cell.Compatible material is usual
For polarity, readily soluble, uncharged small molecule organic material in physiological pH range, wherein, tetrahydropyrimidine be one very
Effective compatible solute, Main Function is the osmotic pressure inside and outside statocyte, and research finds that tetrahydropyrimidine can improve adipose membrane
The hydration on surface strengthens adipose membrane fluidization, improves the ability of cell antagonism extreme environment.Document: Harishchandra R
K,Wulff S,Lentzen G,et al.The effect of compatible solute ectoines on the
structural organization of lipid monolayer and bilayer membranes[J]
.Biophysical Chemistry,2010,150(1-3):37-46.Meanwhile, tetrahydropyrimidine also can be biological big with protein etc.
The protein surface of molecule interacts, and improves the structural stability of the biomacromolecule such as protein, nucleic acid, therefore, at cell
Time under condition of salt stress, tetrahydropyrimidine class material by improving the salt tolerant of cell to the protection of cell membrane, nucleic acid, protein etc.
Property.Tetrahydropyrimidine increasingly receives publicity as the efficient compatible solute of one.
The invention provides a kind of by adding a small amount of tetrahydropyrimidine during anaerobic fermentation of kitchen waste, increase system
System salt resistance ability, reduces the salinity suppression to anaerobic fermentation of kitchen waste, with the method reaching to improve methane yield.Concrete step
Rapid as follows:
(1) changing food waste pre-treatment: carry out sorting and break process by changing food waste.
(2) anaerobic fermentation: the changing food waste through pre-treatment is placed in slaking apparatus, according in reaction system with seed sludge
Na+Concentration adds a certain amount of tetrahydropyrimidine, adds water distribution to certain volume, and in reaction system, salt concentration is that 3g/L is (with Na+
Meter) more than, control anaerobic fermentation temperature, finally draw off biogas residue.
Wherein, described changing food waste uses crusher in crushing to become particle diameter about 5～the slurry of about 10mm.
Wherein, described seed sludge is the anaerobic fermentation mud of various raw material, the preferably anaerobic fermentation of sewage treatment plant
Mud.
Wherein, described anaerobic fermentation of kitchen waste device is homogenous anaerobic fermentation reactor, and the reaction method of operation is permissible
That batch is run: reaction raw materials is disposably put into, confined reaction after inoculation until degradable, final disposable discharging.Fortune
Line mode can also be semi continuous: fixed time intervals adds certain virgin material, discharges certain biogas slurry and biogas residue simultaneously, it is ensured that
Overall balance in system.
Wherein, described changing food waste feed loading is 1.5g (VS)/more than L, and the feed loading of semi-continuous operation is excellent
Select 1.5～5.0g (VS)/L, feed loading preferably 5.0～10g (VS)/L that batch is run.VS represents volatile solid
(Volatile Solid), L represents changing food waste and seed sludge mixeding liquid volume.
Changing food waste adds with seed sludge F/M=0.5 in mass ratio～1.5, preferably F/M=1.F/M represents organic negative
Lotus rate (Food/Microorganism).
Wherein, described tetrahydropyrimidine addition is calculated by Na ion concentration in system, considers changing food waste simultaneously and connects
Planting the factor of mud mixed liquid concentration, in changing food waste and seed sludge mixed liquor, every gram of sodium ion adds tetrahydropyrimidine 0.03-
0.6g, preferably scope are 0.1-0.4g.Wherein, described changing food waste and the mixed liquor of seed sludge and the mass ratio of water, press
In reactor system solid content be 5～10% calculate obtain.Solid content refers to solid constituent in the mixture of anaerobic fermentation system
The percentage by weight of content.
Wherein, described anaerobic fermentation temperature is 33～39 DEG C, preferably 35～37 DEG C.
Whole anaerobic fermentation process need fermentation period 28～35 days.
After adding tetrahydropyrimidine, high salt anaerobic fermentation of kitchen waste total gas production is compared with the total product without tetrahydropyrimidine
Tolerance improves more than 15%, and methane content is 65%～78%, and average content is 72%.System stability index is all at normal model
Enclose.
Compared with prior art, the invention has the beneficial effects as follows: compared to without tetrahydropyrimidine, the present invention can significantly carry
High anaerobic fermentation of kitchen waste total gas production, and ensure stable higher methane content, improve changing food waste into resources and process
Benefit.Compared to without tetrahydropyrimidine, the present invention can alleviate high concentration Na+Suppression shadow to anaerobic fermentation of kitchen waste
Ring, strengthen system salt resistance ability, improve changing food waste utilization ratio.The present invention can alleviate Na in system longtime running+Accumulation is made
The anaerobic fermentation system performance degradation phenomenon become.
Detailed description of the invention
Embodiment
The changing food waste food waste disposer of collection is ground into particle diameter about 5～the slurry of about 10mm；Inoculation
After mud stands 1 day, outwell supernatant, take sediment fraction.
Anaerobic ferment devices be volume be the anaerobic reactor of 250mL, effective volume is 150mL, is placed in constant water bath box
In, keep anaerobic fermentation temperature 35 DEG C ± 2 DEG C, the every day of vibration twice sooner or later, each 30 minutes, use drainage to take gas, reaction
Device keeps good anaerobic environment.
Anaerobic fermentation of kitchen waste organic loading is 10gVS L-1, changing food waste and seed sludge F/M (in terms of VS) are 1,
Anaerobic digestion natural law is 35～40 days.Test arranges two Na+Concentraton gradient, respectively moderate Concentraton gradient 5g/L and height are dense
Degree gradient 10g/L.Tetrahydropyrimidine adds concentration and is 1.0g/L.
Add the anaerobic fermentation of kitchen waste usefulness after tetrahydropyrimidine and be listed in table 1.As can be seen from Table 1, tetrahydrochysene is added phonetic
After pyridine, Na+For in the anaerobic fermentation of kitchen waste system of 5.0g/L, load methane production is compared with adding without tetrahydropyrimidine
15.5%；Na+For in the anaerobic fermentation of kitchen waste system of 10.0g/L, load methane phase tolerance relatively increases without tetrahydropyrimidine
Having added 15.3%, two groups of methane content meansigma methodss are the highest.After reaction terminates, the Na in system+Under concentration all has by a small margin
Fall, and the test group of interpolation tetrahydropyrimidine is compared with the matched group without adding, and declines more notable.
From in table 1, along with Na+Increasing of concentration, system is elongated from the time brought into operation to stable, Na+For
During 5.0g/L, add the test group of tetrahydropyrimidine by system from bringing into operation to the stable time by within 10 days, foreshortening to 7 days, the most instead
30 days have been foreshortened to by 35 days between Ying Shi；Na+During for 10.0g/L, add tetrahydropyrimidine test group by system from bringing into operation
Being foreshortened to 10 days to the stable time by 14 days, total reaction time was foreshortened to 32 days by 38 days；It is therefore seen that interpolation tetrahydropyrimidine
Microorganism can be shortened and adapt to the time of hypersaline environment, strengthen system salt resistance ability.
Table 1 adds the anaerobic fermentation of kitchen waste usefulness after tetrahydropyrimidine
Claims (9)
1. the method improving anaerobic fermentation of kitchen waste salt resistance ability and gas generation property, it is characterised in that include following step
Rapid:
(1) carry out sorting and break process by the changing food waste of collection；
(2) treated changing food waste and seed sludge are placed in slaking apparatus, according to the Na in reaction system+Concentration is added certain
The tetrahydropyrimidine of amount, adds water distribution to certain volume, and in reaction system, salinity is with Na+Concentration is calculated as more than 3g/L, controls anaerobism and sends out
Ferment temperature, finally draws off biogas residue；
Described tetrahydropyrimidine addition is calculated by Na ion concentration in system, according in changing food waste and seed sludge mixed liquor
Every gram of sodium ion adds tetrahydropyrimidine 0.03-0.6g；
The described anaerobic fermentation of kitchen waste reaction method of operation can be that batch is run or semi-continuous operation；
Wherein, described anaerobic fermentation temperature is 33～39 DEG C；
Whole anaerobic fermentation process needs 28～35 days.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described changing food waste processes and uses crusher in crushing to become particle diameter about 5～the slurry of 10mm.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described seed sludge is the anaerobic fermentation mud of the anaerobic fermentation mud of various raw material, preferably sewage treatment plant.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, the mass ratio of changing food waste and seed sludge is pressed F/M=0.5～1.5 and is added.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, wherein, described changing food waste feed loading is 1.5g (VS)/more than L, and the changing food waste of described semi-continuous operation enters
Material load is 1.5～5.0g (VS)/L, changing food waste feed loading 5.0～10g (VS)/L that batch is run.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described changing food waste and the mixed liquor of digested sludge and the mass ratio of water, be 5 by total system solid content in reactor
～10% percetage by weight.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described tetrahydropyrimidine addition adds tetrahydropyrimidine according to every gram of sodium ion in changing food waste and seed sludge mixed liquor
0.1-0.4g。
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 1 and the method for gas generation property, its feature
Being, described anaerobic fermentation temperature is 35～37 DEG C.
Raising anaerobic fermentation of kitchen waste salt resistance ability the most according to claim 4 and the method for gas generation property, its feature
Being, the mass ratio of described changing food waste and seed sludge presses F/M=1.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103725715A (en) * | 2013-12-30 | 2014-04-16 | 北京化工大学 | Preparation method of calcium ion slow-release agent and method for improving anaerobic digestion of kitchen wastes |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103725715A (en) * | 2013-12-30 | 2014-04-16 | 北京化工大学 | Preparation method of calcium ion slow-release agent and method for improving anaerobic digestion of kitchen wastes |
Non-Patent Citations (2)
| Title |
|---|
| 何健: "甜菜碱和四氢嘧啶提高Pseudomonas putida KT2440耐盐性的分子机理研究", 《中国博士论文全文数据库》 * |
| 王魁荣: "一株中度嗜盐菌Halomonas sp. NY-011的耐盐特性及机理", 《应用与环境生物学报》 * |
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