CN106044878B - A kind of blue algae treatment agent and its application method and application - Google Patents
A kind of blue algae treatment agent and its application method and application Download PDFInfo
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- CN106044878B CN106044878B CN201610530195.XA CN201610530195A CN106044878B CN 106044878 B CN106044878 B CN 106044878B CN 201610530195 A CN201610530195 A CN 201610530195A CN 106044878 B CN106044878 B CN 106044878B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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Abstract
The invention discloses a kind of blue algae treatment agent and its application method and application, the blue algae treatment agent, including following components: nano SiO 2 particle and the cationic polyelectrolyte containing amido, the two mass ratio are 20~50: 1.Blue algae treatment agent of the present invention utilizes nano SiO 2 particle compound rapidly with Cells of Blue-green Algae under the cationic polyelectrolyte effect containing amido and forms cyanobacteria aggregation, then cyanobacteria aggregation is made to be deposited to the bottom by the gravity of silica, to inhibit the growth of cyanobacteria, the outburst of cyanobacteria is prevented.
Description
Technical field
The present invention relates to environmental protection and water treatment field, more particularly to a kind of blue algae treatment agent and its application method and
Using.
Background technique
Cyanobacteria is prokaryotes, is called blue-green alge cyanobacteria；There is colloid clothing outside the cell wall of most of cyanobacterias, is called viscous
Algae.In algae bio, cyanobacteria is most simple, most original unicellular organism, and without nucleus, there is nuclear matter in cell center,
It is usually in granular form or netted, pigment is evenly distributed in cytoplasm.Nuclear matter does not have nuclear membrane and kernel, the function with core
Can, therefore referred to as protokaryon (or nucleoid).There is cyclic DNA plasmid in cyanobacteria, takes on the effect of carrier.Extremely due to water resource environment
Badly the phenomenon that leading to cyanobacteria mass propagation and destroying the ecological balance referred to as cyanobacterial bloom.
The outburst of cyanobacterial bloom has become a global problem, and incidence shows an increasing trend year by year.
Many cyanobacterias in nature can form air bag in portion in the cell, these air bags can help them to float in water body to catch
Obtain more sunlights.The cyanobacteria of exactly these floatings gathers on a large scale in the water surface forms wawter bloom.Some species of cyanobacteria, example
Such as Microcystis aeruginosa (Microcystis), anabena (Anabaena) and silk capsule algae (Aphanizomenon), they are in outburst
A series of algae toxin can be discharged into water body, these algae toxins can seriously threaten the health of human and animal, especially have
Very strong hepatotoxicity wind agitation.Importantly, will lead to Dissolved Oxygen in Water after cyanobacterial bloom outburst and decline rapidly, aquatic environment is destroyed
The ecosphere, draw water deficiency.With economic fast development and to the excessive use of water resource, Chinese many lakes
All seriously receive the harm of cyanobacterial bloom.For example, the algal bloom in Taihu Lake in 2007 (China, Jiangsu Province) directly results in
The drinking water crisis of Wuxi City, about five million peoples are affected by it.
In order to avoid endangering with mitigation cyanobacterial bloom bring, people have invented a variety of strategies to remove cyanobacterial bloom.
Most common processing method, first is that using chemical algicide and flocculant, second is that artificial fishing.Both methods, the former
Lack biological selectivity, and secondary pollution can be generated, brings other adverse effects to water environment, and the latter then needs to consume largely
Man power and material, cost is too high.Importantly, the method for the processing cyanobacterial bloom that people use at present is all concentrated mainly on
Processing after algal bloom, and the outburst of cyanobacterial bloom can not be inhibited.Due to the eutrophication of water body be algal bloom most
One of key factor, therefore the phosphorus load capacity reduced in waters and deposit is most widely used at present method, but it is this
Method cost is too high, is also not suitable for widespread adoption.Environmental Technology based on biological interaction is control most modern at present
The method of cyanobacterial bloom outburst, although there is very much prospect, a wide range of Use Limitation fruit is unobvious.So exploitation is safer
The effective method for inhibiting cyanobacterial bloom outburst is a huge challenge for the mankind.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of blue algae treatment agents based on silica.
In the water body of nature, bottom be compared with the water surface it is dark, temperature is usually lower than the water surface (except winter), institute
The photosynthetic growth and cell division that will limit Cells of Blue-green Algae with the environment of bottom, pass through nano SiO 2 particle and Cells of Blue-green Algae
It is compound, so that Cells of Blue-green Algae is spontaneous under the gravity of silica to be deposited to the bottom, to inhibit the photosynthetic life of cyanobacteria
Long and cell division inhibits blue algae bloom.But the cyanobacteria for being usually formed wawter bloom can form air bag, these gas in portion in the cell
Capsule can help them to be floated in water body to capture more sunlights.The cyanobacteria of exactly these floatings collects on a large scale in the water surface
It is poly- to form wawter bloom.Cells of Blue-green Algae surface is usually electronegative, and nano SiO 2 particle is also that band is negative in neutral conditions
Electricity, under the cationic polyelectrolyte effect containing amido, Cells of Blue-green Algae can be compound rapidly with nano SiO 2 particle
And cyanobacteria aggregation is formed, since the density ratio water of silica is big, cyanobacteria/silica complex is deposited to rapidly the bottom.
A kind of blue algae treatment agent, including following components: nano SiO 2 particle and the poly- electrolysis of cation containing amido
Matter, the two mass ratio are 20~50: 1.
Preferably, the mass ratio between nano SiO 2 particle and cationic polyelectrolyte containing amido is 25~35
:1.Most preferably, the mass ratio between nano SiO 2 particle and cationic polyelectrolyte containing amido is 30: 1.
Preferably, the partial size of the nano SiO 2 particle is not more than 100nm.The partial size one of nano SiO 2 particle
As refer to average value, can have fluctuation in a small range.When partial size is too big, effect meeting relative mistake is a little, because can be very fast
Ground sinks to the bottom, and may have part to have little time to interact with cyanobacteria.It is furthermore preferred that the grain of the nano SiO 2 particle
Diameter is 20~30nm.
Polyelectrolyte is the long chain macromolecule with ionogen, and electricity can occur in polar solvent for this kind of macromolecule
From making to take charge on macromolecular chain.Polyelectrolyte with positive or negative charge on chain is called polycation or polyanion respectively.
Polyelectrolyte is respectively provided with electrolyte and high molecular properties.Polyelectrolyte solution is electrically conductive similar to electrolyte solution,
Similar Polymer Solution, there is very big viscosity.Amido is per se on schedule, and amido and silica binding performance are preferable.
Preferably, the molecular weight of the cationic polyelectrolyte containing amido is not more than 100000.With above-mentioned titanium dioxide
Nano silicon particles are the same, and when molecular weight is too big, effect meeting relative mistake is a little, because the bottom can be sunk to quickly, and may have part
Have little time to interact with cyanobacteria.It is furthermore preferred that the cationic polyelectrolyte containing amido is poly dimethyl allyl
One of ammonium chloride, polyethyleneimine or cationic-type polyacrylamide or combination.Wherein, poly dimethyl allyl chlorination
The molecular weight of ammonium is 50000~100000, and the molecular weight of polyethyleneimine is 4500~6500, cationic-type polyacrylamide
Molecular weight is 40000~100000.
Invention further provides the blue algae treatment agents in the application administered in water pollution caused by cyanobacteria.It is of the invention blue
Algae inorganic agent is mainly used for being combined with cyanobacteria living, and the cyanobacteria dead cell for having occurred and that cell wall rupture combines effect
Fruit is poor, thus blue algae treatment agent of the present invention both can when cyanobacteria forms wawter bloom there are no outburst using to be preventive from possible trouble,
It can also be administered after forming wawter bloom, but for forming the dead cell floated after wawter bloom, aggregation settlement action is poor.
Preferably, in the application, the type of the cyanobacteria is one in Microcystis aeruginosa, anabena, silk capsule algae or chlorella
Kind is a variety of.These cyanobacteria types accounting when cyanobacterial bloom occurs is larger, and can all generate algae toxin.
The present invention also provides a kind of methods of water pollution caused by improvement cyanobacteria, comprising the following steps:
(1) cyanobacteria density in water body is detected, when cyanobacteria density is greater than 2 × 104It is judged to needing to handle when a/mL；
(2) blue algae treatment agent as described in claims 1 to 3 is any is sprinkling upon in water body to be processed, blue algae treatment agent
Usage amount is 50~100mg of per cubic meter of water.
Preferably, the method, when use, first are dispersed in water the blue algae treatment agent to form dispersion liquid.Because this
The unit volume water body usage amount of invention blue algae treatment agent is smaller, so blue algae treatment agent is dispersed in the water of certain volume again
It sows more convenient in water, is also easy to sow uniformly.
Blue algae treatment agent of the present invention is using nano SiO 2 particle under the cationic polyelectrolyte effect containing amido
It is compound and form cyanobacteria aggregation rapidly with Cells of Blue-green Algae, then so that cyanobacteria aggregation is deposited to water by the gravity of silica
Bottom prevents the outburst of cyanobacteria to inhibit the growth of cyanobacteria.
Detailed description of the invention
Fig. 1 is that blue algae treatment agent assembles sedimentation result figure to Microcystis aeruginosa in embodiment 1；
Fig. 2 is the optical microphotograph sem observation result figure of Microcystis aeruginosa, wherein figure A is untreated fish group, and figure B is processing group；
Fig. 3 is Microcystis aeruginosa growing state result figure after blue algae treatment agent is handled in embodiment 2, wherein figure A is processing group
Directly observation with untreated fish group is as a result, figure B is the change in cell density result figure of processing group and untreated fish group；
Fig. 4 is Microcystis aeruginosa algae toxin result of variations figure in embodiment 2.
Specific embodiment
Embodiment 1
By partial size be 20~30nm nano SiO 2 particle and polydimethyl diallyl ammonium chloride (PDADMAC, point
Son amount is 50000~100000) acquisition blue algae treatment agent is mixed with mass ratio 20: 1.The addition of 8 μ g blue algae treatment agents there is into 80mL
(microcystis density: 2 × 10 in the beaker of Microcystis aeruginosa suspension6A/milliliter), final silica dioxide granule is in Microcystis aeruginosa suspension
In concentration be 100 μ g/L, as a result as shown in Figure 1, Microcystis aeruginosa rapid aggregation settles, after about 20min, substantially all aggregation is heavy
Drop is in beaker bottom.
Use the cyanobacteria of optical microphotograph sem observation before and after the processing, it can be seen that cyanobacteria is thin before handling using blue algae treatment agent
Intercellular mutually disperses, and after handling, aggregation (Fig. 2) occurs for cyanobacteria.
Embodiment 2
By partial size be 20~30nm nano SiO 2 particle and polydimethyl diallyl ammonium chloride (PDADMAC, point
Son amount is 50000~100000) acquisition blue algae treatment agent is mixed with mass ratio 25: 1.The addition of 20 μ g blue algae treatment agents is had
(microcystis density: 2 × 10 in the culture bottle of 400mL Microcystis aeruginosa suspension6A/milliliter), final silica dioxide granule is micro-
Concentration in capsule algae suspension is 50 μ g/L, and then the Microcystis aeruginosa after observation aggregation sedimentation is rised in value situation, as a result as shown in figure 3,
In 12 days (d) times, Microcystis aeruginosa rapid appreciation not plus in the culture bottle of blue algae treatment agent, and reached plateau at 10 days or so,
Later until the 12nd day, quantity kept stable；And added in the culture bottle of blue algae treatment agent, the quantity of Microcystis aeruginosa is basic
Quantity at the beginning is maintained always, is not rised in value significantly.
The detection discovery of Microcystis aeruginosa algae toxin, within 12 day time, plus in the culture bottle of blue algae treatment agent Microcystis aeruginosa algae is not malicious
Plain concentration increases rapidly；And added in the culture bottle of blue algae treatment agent, Microcystis aeruginosa algae toxin maintains always lower concentration, does not have
There is apparent increase (Fig. 4).
Embodiment 3
By partial size be 50~60nm nano SiO 2 particle and polyethyleneimine (PEI, molecular weight be 4500~
6500) it is mixed with mass ratio 30: 1 and obtains blue algae treatment agent.6.4 μ g blue algae treatment agents are added to the burning for having 80mL anabena suspension
(microcystis density: 2 × 10 in cup6A/milliliter), concentration of the final silica dioxide granule in anabena suspension is 80 μ
G/L, as a result anabena rapid aggregation is deposited in beaker bottom.
Embodiment 4
By partial size be 70~80nm nano SiO 2 particle and cationic-type polyacrylamide (PAM, molecular weight are
40000~100000) it is mixed with mass ratio 50: 1 and obtains blue algae treatment agent.The addition of 4.8 μ g blue algae treatment agents there are into 80mL capsules
(microcystis density: 2 × 10 in the beaker of algae suspension5A/milliliter), final silica dioxide granule is in silk capsule algae suspension
Concentration is 60 μ g/L, and as a result silk capsule algae rapid aggregation is deposited in beaker bottom.
Embodiment 5
By partial size be 90~100nm nano SiO 2 particle and cationic-type polyacrylamide (PAM, molecular weight are
40000~100000) it is mixed with mass ratio 35: 1 and obtains blue algae treatment agent.The addition of 4 μ g blue algae treatment agents there is into 80mL chlorella
(microcystis density: 2 × 10 in the beaker of suspension4A/milliliter), final silica dioxide granule is dense in chlorella suspension
Degree is 50 μ g/L, and as a result chlorella rapid aggregation is deposited in beaker bottom.
Claims (7)
1. a kind of blue algae treatment agent, which is characterized in that including following components: nano SiO 2 particle and containing amido sun from
Sub- polyelectrolyte, the two mass ratio are 20~50: 1,
The partial size of the nano SiO 2 particle is not more than 100nm, the molecule of the cationic polyelectrolyte containing amido
Amount is not more than 100000,
The cationic polyelectrolyte containing amido is polydimethyl diallyl ammonium chloride, polyethyleneimine or cationic
One of polyacrylamide or combination.
2. blue algae treatment agent as described in claim 1, which is characterized in that nano SiO 2 particle and containing amido sun from
Mass ratio between sub- polyelectrolyte is 25~35: 1.
3. blue algae treatment agent as described in claim 1, which is characterized in that the partial size of the nano SiO 2 particle be 20~
30nm。
4. the blue algae treatment agent as described in claims 1 to 3 is any is in the application administered in water pollution caused by cyanobacteria.
5. application as claimed in claim 4, which is characterized in that the type of the cyanobacteria be Microcystis aeruginosa, anabena, silk capsule algae or
One of chlorella is a variety of.
6. a kind of method for administering water pollution caused by cyanobacteria, which comprises the following steps:
(1) cyanobacteria density in water body is detected, when cyanobacteria density is greater than 2 × 104It is judged to needing to handle when a/mL；
(2) blue algae treatment agent as described in claims 1 to 3 is any is sprinkling upon in water body to be processed, blue algae treatment agent uses
Amount is 50~100mg of per cubic meter of water.
7. method as claimed in claim 6, which is characterized in that first be dispersed in water to be formed by the blue algae treatment agent when use
Dispersion liquid.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101327981A (en) * | 2008-08-01 | 2008-12-24 | 孟中 | Active diatom nanometer water purification agent and production method thereof |
| CN103803690A (en) * | 2014-03-05 | 2014-05-21 | 中南大学 | Flocculating agent for simulating composition of microorganism extracellular polymeric substances and application method thereof |
| CN105072904A (en) * | 2013-04-10 | 2015-11-18 | 顺天大学校产学协力团 | Anti-algal bloom composition containing water-soluble free amine chitosan as active ingredient, and method for removing algal-bloom using same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101327981A (en) * | 2008-08-01 | 2008-12-24 | 孟中 | Active diatom nanometer water purification agent and production method thereof |
| CN105072904A (en) * | 2013-04-10 | 2015-11-18 | 顺天大学校产学协力团 | Anti-algal bloom composition containing water-soluble free amine chitosan as active ingredient, and method for removing algal-bloom using same |
| CN103803690A (en) * | 2014-03-05 | 2014-05-21 | 中南大学 | Flocculating agent for simulating composition of microorganism extracellular polymeric substances and application method thereof |
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