CN106039402A - Double-layer periosteum-imitation material and preparation method thereof - Google Patents
Double-layer periosteum-imitation material and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/14—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one polyester as constituent
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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Abstract
The invention relates to a double-layer periosteum-imitation material and a preparation method thereof. The method includes the steps of preparation of an outer-layer spinning solution, preparation of an inner-layer spinning solution and preparation of a double-layer periosteum-imitation film. An electrostatic spinning device is adopted, after a receiving device is grounded, the receiving distance is controlled to be 9-18 cm, the inner-layer spinning solution is put in an injector, positive high pressure is applied, the liquid flow rate is adjusted, and a fiber film with the thickness ranging from about 0.1 mm to 2 mm is obtained; then the inner-layer spinning solution in the injector is changed into the outer-layer spinning solution, positive high pressure is applied, the liquid flow rate is adjusted, and a double-layer electrostatic spinning fiber film with the total thickness ranging from 0.2 mm to 4 mm is obtained. The invention particularly relates to preparation of the electrostatic spinning film of polylactic acid and silk fibroin and adding of bone growth promotion factors. Recovery of bone tissue can be promoted, and the double-layer periosteum-imitation material is novel and has bone generation promotion ability.
Description
Technical field
The present invention relates to a kind of novel double-layer and imitate periosteum material and preparation method thereof, be specifically related to poly-(racemic lactic acid-own interior
Ester) and fibroin albumen static spinning membrane preparation and promote skeletal growth factor interpolation.The imitative periosteum of this bilayer can promote bone
Tissue repair, is that the bilayer that a kind of novel having promotees osteogenesis ability imitates periosteum material.This method belongs to technical field of biological material,
Prepared material can be widely used for bone tissue restoration.
Background technology
Cranial defect is clinical common disease, and its cause of disease comprises wound, infection, tumor, osteomyelitis operation wound clearing and each
Planting congenital diseases etc., these disease incidences are high, and the maximum dose Cranial defect produced due to these diseases or large segmental bone defect
It is difficult to self-healing, needs to carry out bone tissue restoration operation.Means of mainly repairing have three kinds, move including autologous bone
Plant, allogenic bone transplantation and artificial bone reparation.Wherein, autologous bone transplanting clinical effectiveness is optimal, and its major advantage is that it contains
Blood capillary, can allow bone again recover blood supply, thus rapidly promotes knitting at transplantation site, but its limited source, bone piece
Less, form is fixed, and can bring new wound to patient.There is the risk of immunological rejection in allogenic bone transplantation, is easily caused inflammation
Disease, infect, fracture and the complication such as bone does not connect.Therefore, not enough for prior art, develop quantity and do not rely on autologous bone,
Performance is significant close to the artificial bone repair materials Cranial defect clinical for treatment of autologous bone.
The major reason that autologous bone transplanting success rate is high is that it is present in bone surface with complete periosteum, periosteum,
Thickness is less than 1mm, but as important interface, can increase the fusion between bone and surrounding tissue, and the most osteoblastic growth, for bone
Tissue blood supply.In union of fracture, bone defect healing, there is pivotal role.Clinically, only have that quantity is few by Self periosteum, take
The problem that material is difficult.Therefore the imitative periosteum material constructing energy vascularization promoting and osteanagenesis simultaneously is most important.
Periosteum has inside and outside double-layer structure, and outer layer is rich in vasoganglion, makes skeleton active, and participates in Osteoblastic activity, is into
Bone key point；Osteogenesis and hypertrophy are played an important role by internal layer.The most autologous bone of disappearance of periosteum is difficult to self-healing and different
Body bone or the failed major reason of artificial bone repairing bone defect.Therefore, we combine the electrostatic spinning skill of rising in recent years
Art, constructs novel bilayer and imitates periosteum material.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, invent a kind of novel bilayer and imitate periosteum material and preparation method thereof.Should
Bionical periosteum is that tissue growth provides specific biotic environment, utilizes organizational project principle preferably to devise bionical tissue and repaiies
Multiple material, simulates the ad hoc structure of primary periosteum, for tissue repair success the most advantageously.This bilayer periosteum can be used for wrapping
Wrap up in Cranial defect position, for Bone Defect Repari.
For realizing such purpose, the present invention is achieved through the following technical solutions.
A kind of bilayer imitates the preparation method of periosteum material, it is characterised in that comprise the steps:
(1) preparation of outer layer spinning liquid:
Weigh a certain amount of silk fibroin powder (SF), be dissolved in the water, be stirring evenly and then adding into VEGF
(VEGF), continue stirring and be concentrated to give fibroin/VEGF spinning liquid；VEGF/SF mass ratio is 0.002 ‰-0.02 ‰；
(2) preparation of internal layer spinning liquid:
Configuration spinning liquid methylene chloride: dimethylformamide (DMF)=7:3(V/V), add a certain amount of hydroxyapatite
(HA), ultrasonic 20min；Add a certain amount of poly-(racemic lactic acid-caprolactone) copolymer (PDLLA-CL), magnetic agitation mistake
At night, obtain internal layer spinning liquid；HA/PDLLA-CL mass ratio is 5%-20%；
(3) preparation of double-deck imitative periosteum:
Using electrostatic spinning apparatus, after receiving device ground connection, control receiving range is 9-18cm, loads internal layer spinning in syringe
Liquid, adds positive high voltage, regulates flow rate of liquid, obtains the fibrous membrane that thickness is about 0.1-2mm, then changes syringe internal layer spinning liquid
For outer layer spinning liquid, add positive high voltage, regulate flow rate of liquid, obtain the double-deck electrospun fibers film that gross thickness is 0.2-4mm.
In described outer layer spinning liquid, fibroin albumen quality concentration is 20%-35%.
In described internal layer spinning liquid, the mass concentration of PDLLA-CL is 6.3%-7.2%.
Described positive high voltage is 7kV-20kV.
The flow rate of liquid of described outer layer spinning liquid is 0.1-0.8mL/h；The flow rate of liquid of described internal layer spinning liquid is 30mm/
min。
A kind of double-deck imitative periosteum material, it is characterised in that prepare according to any of the above-described described method.The present invention's is excellent
Gesture is: the biomimetic features of the inside and outside bilayer of double-deck imitative periosteum in (1) present invention, its energy of each department, has preferable Bone formation
Ability；(2) angiogenic growth factor of outer layer fibroin albumen load, can promote angiogenesis, recover blood supply faster, with oneself
Body bone carries out nutrient substance exchange；Internal layer is ploy DL lactic caid-caprolactone composite hydroxylapatite, not only makes periosteum have relatively
High intensity and HA have and preferably promote bone growth capacity；(3) duplicature uses electrostatic spinning technique to be prepared from, and has
The hole of 100-400mm, can more preferably simulate growth and the infiltration of the micro nanometer fiber network structure of periosteum, more conducively osteocyte,
Thus beneficially osteanagenesis and reparation.
Accompanying drawing explanation
Accompanying drawing 1 is the Electronic Speculum figure (SEM) of the inner layer film of double-deck imitative periosteum, and in figure, particulate material is HA.
Accompanying drawing 2 is the Electronic Speculum figure (SEM) of the outer membrane of double-deck imitative periosteum.
Detailed description of the invention
Below by way of specific embodiment, technical scheme is further described.Below example is to this
Further illustrating of invention, and do not limit the scope of the invention.
Embodiment 1:
The preparation of outer layer spinning liquid
Weigh 2g silk fibroin powder (SF), be dissolved in 8ml water, be stirring evenly and then adding into VEGF (VEGF),
Continue stirring and be concentrated to give fibroin/VEGF spinning liquid；
The preparation of internal layer spinning liquid
Configuration spinning liquid methylene chloride: dimethylformamide (DMF)=7:3(V/V), take 10ml, add hydroxyapatite
(HA) 0.06g, ultrasonic 20min.Add 0.6g poly-(racemic lactic acid-caprolactone) copolymer (PDLLA-CL), magnetic agitation mistake
At night, obtain internal layer spinning liquid.
The preparation of double-deck imitative periosteum
Using electrostatic spinning apparatus, after receiving device ground connection, control receiving range is 10cm, loads internal layer spinning in syringe
Liquid, adds positive high voltage, and regulation flow rate of liquid is 30mm, and No. 4 flat syringe needles obtain the fibrous membrane that thickness is about 1mm, then change injection
Device internal layer spinning liquid is outer layer spinning liquid, adds positive high voltage, regulates flow rate of liquid 0.2ml/h, obtains the bilayer that gross thickness is 2mm quiet
Electricity spinning fibre film；
Embodiment 2:
The preparation of outer layer spinning liquid
Weigh 3g silk fibroin powder (SF), be dissolved in 10ml water, be stirring evenly and then adding into VEGF (VEGF),
Continue stirring and be concentrated to give fibroin/VEGF spinning liquid；
The preparation of internal layer spinning liquid
Configuration spinning liquid methylene chloride: dimethylformamide (DMF)=7:3(V/V), take 10ml, add hydroxyapatite
(HA) 0.05g, ultrasonic 20min.Add 0.65g poly-(racemic lactic acid-caprolactone) copolymer (PDLLA-CL), magnetic agitation mistake
At night, obtain internal layer spinning liquid.
The preparation of double-deck imitative periosteum
Using electrostatic spinning apparatus, after receiving device ground connection, control receiving range is 13cm, loads internal layer spinning in syringe
Liquid, adds positive high voltage, and regulation flow rate of liquid is 30mm, and No. 4 flat syringe needles obtain the fibrous membrane that thickness is about 2mm, then change injection
Device internal layer spinning liquid is outer layer spinning liquid, adds positive high voltage, regulates flow rate of liquid 0.3ml/h, obtains the bilayer that gross thickness is 4mm quiet
Electricity spinning fibre film；
Embodiment 3:
The preparation of outer layer spinning liquid
Weigh 2g silk fibroin powder (SF), be dissolved in 10ml water, be stirring evenly and then adding into VEGF (VEGF),
Continue stirring and be concentrated to give fibroin/VEGF spinning liquid；
The preparation of internal layer spinning liquid
Configuration spinning liquid methylene chloride: dimethylformamide (DMF)=7:3(V/V), take 10ml, add hydroxyapatite
(HA) 0.12g, ultrasonic 20min.Add 0.6g poly-(racemic lactic acid-caprolactone) copolymer (PDLLA-CL), magnetic agitation mistake
At night, obtain internal layer spinning liquid.
The preparation of double-deck imitative periosteum
Using electrostatic spinning apparatus, after receiving device ground connection, control receiving range is 12cm, loads internal layer spinning in syringe
Liquid, adds positive high voltage, and regulation flow rate of liquid is 30mm, and No. 4 flat syringe needles obtain the fibrous membrane that thickness is about 0.5mm, then change note
Emitter internal layer spinning liquid is outer layer spinning liquid, adds positive high voltage, regulates flow rate of liquid 0.2ml/h, obtains the bilayer that gross thickness is 1mm
Electrospun fibers film.
Claims (6)
1. a bilayer imitates the preparation method of periosteum material, it is characterised in that comprise the steps:
(1) preparation of outer layer spinning liquid:
Weigh a certain amount of silk fibroin powder (SF), be dissolved in the water, be stirring evenly and then adding into VEGF
(VEGF), continue stirring and be concentrated to give fibroin/VEGF spinning liquid；VEGF/SF mass ratio is 0.002 ‰-0.02 ‰；
(2) preparation of internal layer spinning liquid:
Configuration spinning liquid methylene chloride: dimethylformamide (DMF)=7:3(V/V), add a certain amount of hydroxyapatite
(HA), ultrasonic 20min；Add a certain amount of poly-(racemic lactic acid-caprolactone) copolymer (PDLLA-CL), magnetic agitation mistake
At night, obtain internal layer spinning liquid；HA/PDLLA-CL mass ratio is 5%-20%；
(3) preparation of double-deck imitative periosteum:
Using electrostatic spinning apparatus, after receiving device ground connection, control receiving range is 9-18cm, loads internal layer spinning in syringe
Liquid, adds positive high voltage, regulates flow rate of liquid, obtains the fibrous membrane that thickness is about 0.1-2mm, then changes syringe internal layer spinning liquid
For outer layer spinning liquid, add positive high voltage, regulate flow rate of liquid, obtain the double-deck electrospun fibers film that gross thickness is 0.2-4mm.
A kind of bilayer imitates the preparation method of periosteum material, it is characterised in that described outer layer spinning liquid
Middle fibroin albumen quality concentration is 20%-35%.
A kind of bilayer imitates the preparation method of periosteum material, it is characterised in that described internal layer spinning liquid
The mass concentration of middle PDLLA-CL is 6.3%-7.2%.
A kind of bilayer imitates the preparation method of periosteum material, it is characterised in that described positive high voltage is
7kV-20kV。
A kind of bilayer imitates the preparation method of periosteum material, it is characterised in that described outer layer spinning liquid
Flow rate of liquid be 0.1-0.8mL/h；The flow rate of liquid of described internal layer spinning liquid is 30mm/min.
6. a bilayer imitates periosteum material, it is characterised in that prepare according to method described in any of the above-described claim.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107693845A (en) * | 2017-08-08 | 2018-02-16 | 上海纳米技术及应用国家工程研究中心有限公司 | The bilayer that ectonexine loads HAp and VEGF respectively imitates periosteum material |
| CN107812234A (en) * | 2017-10-19 | 2018-03-20 | 上海纳米技术及应用国家工程研究中心有限公司 | Periosteum material with tissue oxygen-supplying function and its preparation method and application |
| CN107823704A (en) * | 2017-12-28 | 2018-03-23 | 广东泰宝医疗器械技术研究院有限公司 | A kind of paradenlal tissue regeneration repair membrane and preparation method thereof |
| CN107875442A (en) * | 2017-10-31 | 2018-04-06 | 上海纳米技术及应用国家工程研究中心有限公司 | Shell/cored structure（Fibroin albumen hydroxyapatite）/ poly-（Racemic lactic acid co caprolactones）The preparation method of tunica fibrosa |
| CN108653816A (en) * | 2018-03-16 | 2018-10-16 | 江阴金泰克生物技术有限公司 | A method of double-layer artificial antibacterial repair membrane is prepared using electrostatic spinning |
| CN110201226A (en) * | 2019-06-28 | 2019-09-06 | 苏州大学附属第一医院 | A kind of micro-/ nano Bionics Bone membrane material and preparation method thereof |
| WO2020077551A1 (en) * | 2018-10-15 | 2020-04-23 | 南通纺织丝绸产业技术研究院 | Composite barrier film and preparation method therefor |
| CN111265722A (en) * | 2020-04-03 | 2020-06-12 | 东华大学 | Double-layer structured periosteum for diabetic bone repair and preparation method thereof |
| CN114525599A (en) * | 2022-03-17 | 2022-05-24 | 北京市创伤骨科研究所 | Bionic periosteum and preparation method and application thereof |
| CN114984314A (en) * | 2022-05-30 | 2022-09-02 | 苏州大学 | Silk fibroin-based bionic periosteum-bone graft and preparation method and application thereof |
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| CN107693845A (en) * | 2017-08-08 | 2018-02-16 | 上海纳米技术及应用国家工程研究中心有限公司 | The bilayer that ectonexine loads HAp and VEGF respectively imitates periosteum material |
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| CN107875442A (en) * | 2017-10-31 | 2018-04-06 | 上海纳米技术及应用国家工程研究中心有限公司 | Shell/cored structure（Fibroin albumen hydroxyapatite）/ poly-（Racemic lactic acid co caprolactones）The preparation method of tunica fibrosa |
| CN107875442B (en) * | 2017-10-31 | 2020-12-29 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method of shell/core structure (silk fibroin-hydroxyapatite)/poly (racemic lactic acid-co-caprolactone) fiber membrane |
| CN107823704A (en) * | 2017-12-28 | 2018-03-23 | 广东泰宝医疗器械技术研究院有限公司 | A kind of paradenlal tissue regeneration repair membrane and preparation method thereof |
| CN108653816A (en) * | 2018-03-16 | 2018-10-16 | 江阴金泰克生物技术有限公司 | A method of double-layer artificial antibacterial repair membrane is prepared using electrostatic spinning |
| WO2020077551A1 (en) * | 2018-10-15 | 2020-04-23 | 南通纺织丝绸产业技术研究院 | Composite barrier film and preparation method therefor |
| CN110201226A (en) * | 2019-06-28 | 2019-09-06 | 苏州大学附属第一医院 | A kind of micro-/ nano Bionics Bone membrane material and preparation method thereof |
| CN111265722A (en) * | 2020-04-03 | 2020-06-12 | 东华大学 | Double-layer structured periosteum for diabetic bone repair and preparation method thereof |
| CN111265722B (en) * | 2020-04-03 | 2021-08-03 | 东华大学 | Double-layer structured periosteum for diabetic bone repair and preparation method thereof |
| CN114525599A (en) * | 2022-03-17 | 2022-05-24 | 北京市创伤骨科研究所 | Bionic periosteum and preparation method and application thereof |
| CN114984314A (en) * | 2022-05-30 | 2022-09-02 | 苏州大学 | Silk fibroin-based bionic periosteum-bone graft and preparation method and application thereof |
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