CN106039094A - Use of bamboo leaf extract in prevention and treatment on alcoholic liver injury - Google Patents

Use of bamboo leaf extract in prevention and treatment on alcoholic liver injury Download PDF

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CN106039094A
CN106039094A CN201610633775.1A CN201610633775A CN106039094A CN 106039094 A CN106039094 A CN 106039094A CN 201610633775 A CN201610633775 A CN 201610633775A CN 106039094 A CN106039094 A CN 106039094A
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adds
leachate
bamboo leaf
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CN106039094B (en
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许海良
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Shanghai Haizhong Industrial Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/899Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10Preparation or pretreatment of starting material
    • A61K2236/19Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/51Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/53Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)
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Abstract

The invention relates to the field of pharmacy and especially relates to a use of bamboo leaf extract in preparation of a drug or food for prevention and treatment on alcoholic liver injury. The bamboo leaf extract contains 2-10wt% of total flavones from bamboo leaves.

Description

The application of Folium Bambosae extract preventing and treating alcoholic liver injury
Technical field
The present invention relates to Medicines and Health Product field, particularly relate to the medical usage of a Plant Extracts.
Background technology
Bamboo has long edible and medicinal history in China, and the medical treatment of bamboo is in the public eye already with health-care effect. According to medical book record, Folium Bambusae have the cool heart delay spleen, clearly quench the thirst, the function of heat-clearing and toxic substances removing.
Folium Bambusae contains substantial amounts of flavone compound and bioactive polysaccharide and other effective ingredient, such as phenolic acids chemical combination Thing, anthraquinone analog compound, terpene lactone, the extraordinary material such as aminoacid and bioactive peptide, manganese, zinc, selenium.
The most conventional Folium Bambusae extraction process has decocting in water extraction, microwave radiation exaraction, organic solvent extraction and macroporous resin Purifies and separates etc., final extract is mainly flavonoid.
Alcoholic liver injury (alcoholic liver disease) shows various, and the initial stage is usually expressed as fatty liver, enters And alcoholic hepatitis, alcoholic fibrosis and alcoholic cirrhosis can be developed into.Extensive liver can be induced thin when serious excessive drinking Even the downright bad liver failure of born of the same parents.
Summary of the invention
The purpose of the present invention aims to provide the medical usage of a kind of Folium Bambosae extract.
Specifically, the invention provides a kind of Folium Bambosae extract and prevent and treat medicine or the food of alcoholic liver injury in preparation In application, described Folium Bambosae extract contains 2~10wt% Folium Bambusae total flavones.
In a preference, the content of described Folium Bambusae total flavones is 5.8wt%.
In another preference, described Folium Bambosae extract comprises the following steps:
Step one, is broken to 20~100 mesh by bamboo leaf powder, puts into reactor, and add bamboo leaf powder weight 2~4 times amount uses second The mixed solution that alcohol is formed according to the volume ratio of 2:1 with water, soaks 4~12 hours;
Step 2, adds the water of bamboo leaf powder weight 2~4 times amount in a kettle., is heated with stirring to reactor temperature and reaches To 40~45 DEG C, adding cellulase, the consumption of described cellulase is that every kilogram of bamboo leaf powder adds cellulase 15~500,000 Unit, stirs with the rotating speeds of 50~100 turns per minute, react 12~36 hours, control reactor temperature 45~55 DEG C it Between;
Step 3, stop heating and stirring, staticly settle 4~8 hours, leach leachate M standby, retain solid residue in In reactor;
Step 4, adds and the water of leachate M equivalent in a kettle., be heated with stirring to reactor temperature reach 40~ 45 DEG C, the dextrin that will be equivalent to bamboo leaf powder weight 0.08~0.15 times amount mixes according to the weight ratio of 1:2 with water, dissolves, and adds α Amylase, the consumption of described alpha amylase is that every kilogram of dextrin adds alpha amylase 20,000 unit, stirs, is thrown by this mixture Enter reactor, stir with the rotating speeds of 50~100 turns per minute, react 12~36 hours, control reactor temperature 45~55 Between DEG C;
Step 5, stops heating and stirring, staticly settles 4~8 hours, leach leachate N;
Step 6, mixes leachate M and leachate N, reclaims ethanol, concentrates, and is dried.
The details of various aspects of the present invention will be able to detailed description in chapters and sections subsequently.By hereafter and claim Description, the feature of the present invention, purpose and advantage will become apparent from.
Accompanying drawing explanation
Fig. 1 is the mouse feeding scheme of embodiment 4
Fig. 2 is each group mice serum aspartate amino transferase contrast of embodiment 4
Fig. 3 is each group mice alanine aminotransferase contrast of embodiment 4
Fig. 4 is each group mouse liver triglyceride contrast of embodiment 4
Detailed description of the invention
The appearance part of the present invention is to have been surprisingly found that based on such a: extract the Folium Bambusae obtained by the new technology of the present invention Extract can effectively treat Alcoholic Hepatic Injury.Therefore, this plant extract can be expected to be developed into a kind of prevention or Person treats the medicine of alcoholic liver injury.
Specifically, the Folium Bambosae extract of the present invention can be extracted from Folium Bambusae by the following new technology of the present invention and obtain:
Step one, is broken to 20~100 mesh by bamboo leaf powder, puts into reactor, and add bamboo leaf powder weight 2~4 times amount uses second The mixed solution that alcohol is formed according to the volume ratio of 2:1 with water, soaks 4~12 hours;
Step 2, adds the water of bamboo leaf powder weight 2~4 times amount in a kettle., is heated with stirring to reactor temperature and reaches To 40~45 DEG C, adding cellulase, the consumption of described cellulase is that every kilogram of bamboo leaf powder adds cellulase 15~500,000 Unit, stirs with the rotating speeds of 50~100 turns per minute, react 12~36 hours, control reactor temperature 45~55 DEG C it Between;
Step 3, stop heating and stirring, staticly settle 4~8 hours, leach leachate M standby, retain solid residue in In reactor;
Step 4, adds and the water of leachate M equivalent in a kettle., be heated with stirring to reactor temperature reach 40~ 45 DEG C, the dextrin that will be equivalent to bamboo leaf powder weight 0.08~0.15 times amount mixes according to the weight ratio of 1:2 with water, dissolves, and adds α Amylase, the consumption of described alpha amylase is that every kilogram of dextrin adds alpha amylase 20,000 unit, stirs, is thrown by this mixture Enter reactor, stir with the rotating speeds of 50~100 turns per minute, react 12~36 hours, control reactor temperature 45~55 Between DEG C;
Step 5, stops heating and stirring, staticly settles 4~8 hours, leach leachate N;
Step 6, mixes leachate M and leachate N, reclaims ethanol, concentrates, and is dried.
Confirming after testing, in the Folium Bambosae extract of the present invention, the content of Folium Bambusae total flavones is 2~10wt%, preferred content For 5.8wt%.
The Folium Bambosae extract of the present invention can be used alone or uses with the form of pharmaceutical composition.Pharmaceutical composition includes The Folium Bambosae extract of the present invention and pharmaceutically suitable carrier as active component.It is preferred that the pharmaceutical composition of the present invention contains The Folium Bambosae extract of the present invention as active component of 0.1-99.9% percentage by weight." pharmaceutically suitable carrier " will not destroy this The pharmaceutical active of Folium Bambosae extract of invention, its effective dose simultaneously, consumption when can play pharmaceutical carrier effect is to human body Nontoxic.
Described pharmaceutically suitable carrier includes but not limited to: soft phospholipid, aluminium stearate, aluminium oxide, ion exchange material, self emulsifying Drug delivery system, tween or other surfactants, serum albumin, buffer substance such as phosphate, glycine, sorbic acid, Water, salt, electrolyte such as sulfate protamine, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salt, magnesium silicate, satisfied fatty acid Partial glyceride mixtures etc..
Other conventional excipient substance such as binding agent (such as microcrystalline Cellulose), filleies are (such as starch, glucose, anhydrous lactitol Sugar and lactose beadlet), disintegrating agent is (such as cross-linked pvp, crosslinked carboxymethyl fecula sodium, cross-linking sodium carboxymethyl cellulose, low-substituted hydroxypropyl Base cellulose), lubricant (such as magnesium stearate) and absorption enhancer, absorption carrier, flavouring agent, sweeting agent, excipient, dilution Agent, wetting agent etc..
The Folium Bambosae extract of the present invention and its pharmaceutical composition can be prepared by this area conventional method and can pass through intestinal Road or non-bowel or topical routes.Oral formulations includes capsule, tablet, oral liquid, granule, pill, powder, pellet Agent, unguentum etc.;Non-intestinal drug delivery agent includes injection etc.;Local administration preparation includes cream, patch, ointment, spray Deng.It is preferably oral formulations.
The route of administration of the Folium Bambosae extract of the present invention and its pharmaceutical composition can be oral, Sublingual, percutaneous, through flesh Meat or subcutaneous, mucocutaneous, vein, urethra, vagina etc..
In addition to making medicament, also can add antioxidant, pigment, enzyme preparation etc. in the Folium Bambosae extract of the present invention Various food additive, make health food by the conventional method of this area.
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention Rather than restriction the scope of the present invention.The experimental technique of unreceipted actual conditions in the following example, generally according to conventional strip Part or according to the condition proposed by manufacturer.Unless otherwise indicated, the most all of percent, ratio, ratio or number are pressed Weight meter.
Unless otherwise defined, the meaning that all specialties used in literary composition are familiar with one skilled in the art with scientific words Justice is identical.Additionally, any method similar or impartial to described content and material all can be applicable in the inventive method.Wen Zhong Described preferable implementation only presents a demonstration with material and is used.
The features described above that the present invention mentions, or the feature that embodiment is mentioned can be in any combination.Patent specification is taken off The all features shown can be with any composition forms use, and each feature disclosed in description any can provide phase The alternative characteristics of purpose same, impartial or similar replaces.Therefore except having special instruction, disclosed feature to be only impartial or similar The general example of feature.
Embodiment 1 prepares Folium Bambosae extract A
Step one, is broken to 40 mesh by bamboo leaf powder, takes 120kg Folium Bambusae dry powder and puts into reactor, adds 360kg alcohol,diluted (alcohol: water=2:1), soaks 6 hours;
Step 2, adds 360kg water in a kettle., is heated with stirring to reactor temperature and reaches 45 DEG C, add fiber Element enzyme 60,000,000 unit, stirs with the rotating speeds of 50 turns per minute, reacts 24 hours, controls reactor temperature at 50 DEG C;
Step 3, stops heating and stirring, staticly settles 6 hours, leach leachate M 500kg standby, retain solid residual Slag is in reactor;
Step 4, adds 500kg water in a kettle., is heated with stirring to reactor temperature and reaches 45 DEG C, stuck with paste by 12kg Essence mixes with 24kg water, dissolves, and adds alpha amylase 240,000 unit, stirs, and this mixture is put into reactor, with every point The rotating speed stirring that clock is 50 turns, reacts 24 hours, controls reactor temperature at 50 DEG C;
Step 5, stops heating and stirring, staticly settles 6 hours, leach leachate N 450kg;
Step 6, mixes leachate M and leachate N, reclaims ethanol, concentrates, and is dried to obtain Folium Bambosae extract 18kg.
With the content of total flavones in this Folium Bambosae extract of spectrophotometry:
Weigh 13.2mg rutin, with 60% ethanol constant volume to 25ml volumetric flask as standard solution.
Accurately draw 0,0.4,0.8,1.2,1.6,2.0ml rutin standard solution, put into 10 milliliters of volumetric flasks and (write mark exactly Note), be separately added into 2.0,1.6,1.2,0.8,0.4,60% ethanol solution of 0ml;Add 5% sodium nitrite solution 0.5ml Shake up, place 6min;Add 10% aluminum nitrate solution 0.5ml, after placing 6min;Add 4% sodium hydroxide solution 4.0ml, add 60% ethanol constant volume, after shaking up, places 15min;(absorption maximum is found in scanning) measures absorbance at 510nm.Make with 0.0ml For blank, by the concentration of rutin content as abscissa, vertical coordinate, as absorbance corresponding under finite concentration, makees standard bent Line.
Total flavones extracts and measures and calculate
Weigh 0.6~0.8g sample powder, add 60ml 60% ethanol and be put in 100ml round-bottomed flask, be placed in water-bath On, reflux, extract, 60min under the conditions of 70 DEG C.Filtration, constant volume are in 100ml.
Pipette samples amount (adjusting according to the absorbance of sample) 1.0ml, puts into 10 milliliters of volumetric flasks (writing labelling exactly), uses 60% ethanol is added to 2.0ml;Add 5% sodium nitrite solution 0.5ml to shake up, place 6min;Add 10% aluminum nitrate, solution 0.5ml, after placing 6min;Add 4% sodium hydroxide solution 4.0ml, after shaking up, add 60% ethanol constant volume, place 15min;? Absorbance is measured at 510nm.The content of total flavones is calculated according to standard curve.
General flavone content=A/M*100 × extension rate
A is the content in standard curve.
After testing, the Folium Bambosae extract of the present invention (5.8wt%) Han total flavones 1.05kg.
Embodiment 2 prepares Folium Bambosae extract B
Step one, is broken to 20 mesh by bamboo leaf powder, takes 80kg Folium Bambusae dry powder and puts into reactor, adds 320kg alcohol,diluted (alcohol: water=2:1), soaks 4 hours;
Step 2, adds 320kg water in a kettle., is heated with stirring to reactor temperature and reaches 40 DEG C, add fiber Element enzyme 12,000,000 unit, stirs with the rotating speeds of 80 turns per minute, reacts 12 hours, controls reactor temperature at 45 DEG C;
Step 3, stops heating and stirring, staticly settles 4 hours, leach leachate M 450kg standby, retain solid residual Slag is in reactor;
Step 4, adds 450kg water in a kettle., is heated with stirring to reactor temperature and reaches 45 DEG C, stuck with paste by 6.4kg Essence mixes with 12.8kg water, dissolves, and adds alpha amylase 12.8 ten thousand unit, stirs, and this mixture is put into reactor, with The rotating speed stirring of 80 turns per minute, reacts 12 hours, controls reactor temperature at 45 DEG C;
Step 5, stops heating and stirring, staticly settles 4 hours, leach leachate N 500kg;
Step 6, mixes leachate M and leachate N, reclaims ethanol, concentrates, and is dried to obtain Folium Bambosae extract 10.5kg.
After testing (method is with embodiment 1), this Folium Bambosae extract (2.2wt%) Han total flavones 0.23kg.
Embodiment 3 prepares Folium Bambosae extract C
Step one, is broken to 100 mesh by bamboo leaf powder, takes 100kg Folium Bambusae dry powder and puts into reactor, adds 200kg alcohol,diluted (alcohol: water=2:1), soaks 12 hours;
Step 2, adds 200kg water in a kettle., is heated with stirring to reactor temperature and reaches 40 DEG C, add fiber Element enzyme 30,000,000 unit, stirs with the rotating speeds of 100 turns per minute, reacts 36 hours, controls reactor temperature at 55 DEG C;
Step 3, stops heating and stirring, staticly settles 8 hours, leach leachate M 300kg standby, retain solid residual Slag is in reactor;
Step 4, adds 300kg water in a kettle., is heated with stirring to reactor temperature and reaches 45 DEG C, stuck with paste by 15kg Essence mixes with 30kg water, dissolves, and adds alpha amylase 300,000 unit, stirs, and this mixture is put into reactor, with every point The rotating speed stirring that clock is 100 turns, reacts 36 hours, controls reactor temperature at 55 DEG C;
Step 5, stops heating and stirring, staticly settles 8 hours, leach leachate N 350kg;
Step 6, mixes leachate M and leachate N, reclaims ethanol, concentrates, and is dried to obtain Folium Bambosae extract 12.5kg.
After testing (method is with embodiment 1), this Folium Bambosae extract (9.6wt%) Han total flavones 1.2kg.
The treatment Alcoholic Hepatic Injury test of embodiment 4 Folium Bambosae extract
One, animal prepares
48 mices are randomly divided into 6 groups, often group 8, numbering G1, G2, G3, G4, G5, G6, respectively blank group With ethanol group and 4 test group, often 10 mices of group.
Two, process of the test
Folium Bambosae extract A, B and C (preparing as embodiment 1,2 and 3 respectively) of the present invention are made into respectively as listed by table 1 below Concentration, feeds 3 weeks altogether.Feed with the Lieber-Decarli liquid mice grain that Dyets company produces, feeding and dosage regimen See Fig. 1, table 1.Testing first 5 days, every day feeds mice with liquid feed, tests the 6th day and starts continuous 10 days, and naive mice depends on Old feeding with liquid feed, ethanol group and test group are added the ethanol of 5% in feedstuff and are fed, simultaneously at the drinking water of test group The Folium Bambosae extract of the corresponding percentage ratio of middle interpolation, in the last day of test, to ethanol group and test group by 35% ethanol gavage, Blank group is with maltodextrin gavage, and gavage is after 9 hours, and anesthetized mice takes blood and other tissues to be measured, and freezen protective is stand-by.
Table 1 mice group dosage
Group Feeding medicine Concentration mg/mL
G1 Blank group 0
G2 Ethanol group 0
G3 Folium Bambosae extract A 5%
G3 Folium Bambosae extract B 3%
G5 Folium Bambosae extract B 5%
G6 Folium Bambosae extract C 5%
Three, experimental result
1, AST, ALT measure
Performance rate method is used to measure mice serum aspartate amino transferase (AST), alanine aminotransferase (ALT), test kit is purchased from Shanghai Shensuo Youfu Medical Diagnosis Products Co., Ltd..Result is as shown in Figure 2 and Figure 3.
2, triglyceride measures
Using enzymatic assays mouse liver triglyceride, test kit helps the good fortune limited public affairs of medical diagnosis articles for use purchased from Shanghai claim Department.Result is as shown in Figure 4.
Four, conclusion:
Result of the test shows: compared with blank group, and ethanol can result in liver injury, and (serum transaminase raises, liver Dirty triglyceride raises), the Folium Bambosae extract of the present invention of various dose can alleviate the serum transaminase rising that ethanol causes And fatty liver, it was demonstrated that the liver injury that ethanol is caused by the Folium Bambosae extract of the present invention has certain treatment function.
Many aspects involved in the present invention have been done as explained above.However, it should be understood that without departing from present invention spirit Putting, those skilled in the art can carry out equivalent to it and change and modify, and described change and modification fall into the application equally before The coverage of claims.

Claims (3)

1. Folium Bambosae extract application in the medicine or food of preparation preventing and treating alcoholic liver injury, described Folium Bambosae extract contains 2~10wt% Folium Bambusae total flavones.
2. the application of Folium Bambosae extract as claimed in claim 1, it is characterised in that the content of described Folium Bambusae total flavones is 5.8wt%.
3. the application of Folium Bambosae extract as claimed in claim 1, it is characterised in that described Folium Bambosae extract is made by the following method :
Step one, is broken to 20~100 mesh by bamboo leaf powder, puts into reactor, add bamboo leaf powder weight 2~4 times amount with ethanol with The mixed solution that water is formed according to the volume ratio of 2:1, soaks 4~12 hours;
Step 2, adds the water of bamboo leaf powder weight 2~4 times amount in a kettle., is heated with stirring to reactor temperature and reaches 40 ~45 DEG C, adding cellulase, the consumption of described cellulase is that every kilogram of bamboo leaf powder adds cellulase 15~500,000 unit, Stir with the rotating speeds of 50~100 turns per minute, react 12~36 hours, control reactor temperature between 45~55 DEG C;
Step 3, stops heating and stirring, staticly settles 4~8 hours, leach leachate M standby, retains solid residue in reaction In still;
Step 4, adds the water with leachate M equivalent in a kettle., is heated with stirring to reactor temperature and reaches 40~45 DEG C, the dextrin that will be equivalent to bamboo leaf powder weight 0.08~0.15 times amount mixes according to the weight ratio of 1:2 with water, dissolves, and adds α and forms sediment Powder enzyme, the consumption of described alpha amylase is that every kilogram of dextrin adds alpha amylase 20,000 unit, stirs, is put into by this mixture Reactor, stirs with the rotating speeds of 50~100 turns per minute, reacts 12~36 hours, controls reactor temperature at 45~55 DEG C Between;
Step 5, stops heating and stirring, staticly settles 4~8 hours, leach leachate N;
Step 6, mixes leachate M and leachate N, reclaims ethanol, concentrates, and is dried.
CN201610633775.1A 2016-08-04 2016-08-04 Application of bamboo leaf extract in preventing and treating alcoholic liver injury Expired - Fee Related CN106039094B (en)

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