CN106038644A - A method of preparing senecio dianthus franch total flavonoids and applications of the total flavonoids - Google Patents
A method of preparing senecio dianthus franch total flavonoids and applications of the total flavonoids Download PDFInfo
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- CN106038644A CN106038644A CN201610306676.2A CN201610306676A CN106038644A CN 106038644 A CN106038644 A CN 106038644A CN 201610306676 A CN201610306676 A CN 201610306676A CN 106038644 A CN106038644 A CN 106038644A
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- total flavones
- senecio
- dianthus
- senecio dianthus
- total flavonoids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention belongs to the technical field of medicines, and particularly relates to a method of preparing senecio dianthus franch total flavonoids and applications of the total flavonoids. The method includes a) drying senecio dianthus franch at 60 DEG C, and crushing to obtain crude powder, b) performing microwave-ultrasonic wave extraction, c) purifying with macroporous adsorptive resin, eluting with ethanol and collecting eluates, and d) concentrating and drying to obtain the total flavonoids. Pharmacodynamic evaluation proves that the extract has good oxidation resistance and obvious effects, can be used for preparing antioxidant medicines and has advantages of obvious curative effects, using convenience, and the like.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to the preparation method and applications of a kind of Senecio dianthus total flavones.
Background technology
Free radical is the mesostate of multiple biochemical reaction in human life activity.Cell is in normal metabolic process
In, or by high-energy radiation, and hyperbaric oxygen, medicine, smoke from cigarette and photochemical air pollution etc. act on, and all can be produced from
By base.
Research shows, free radical is from producing to the process that the process become feeble and die is exactly electron transfer.In life system, electronics
Transfer be a kind of most basic motion, and oxygen electronic capability is very strong, therefore, in organism many chemical reactions all with oxygen
Relevant.Research finds that the oxygen carrier that the free radical of infringement health is nearly all stronger with those activity is relevant, with these things
The free radical that matter combines is called reactive oxygen free radical.The infringement actually one of human body was aoxidized by reactive oxygen free radical
Journey.Therefore, the infringement of free radical to be reduced is it is necessary to do from antioxidation.
Tibet medicinal plants Senecio dianthus is the herbaceos perennial of a kind of Compositae Senecio, has heat-clearing and toxic substances removing
Specially good effect.Senecio dianthus is used as beauty treatment by some Tibetan compatriot in recent years, respond well.Flavone compound have blood fat reducing,
Defying age, antioxidation, antithrombotic, removing free radical, arrhythmia, the effect such as anticancer.
Traditional extracting method includes solvent extraction, separation by precipitation, steam distillation, molecular distillation technique etc..Though
So these methods are used by most people in natural product extraction, but there is also many shortcomings, commonly use during extracting
A large amount of organic solvents；The process time is long；Operating procedure is many.Thus sample loss is serious in experimentation, the error of generation is relatively
Greatly.
The present invention uses the microwave-ultrasound assisted extraction technique in modern times, extraction Senecio dianthus total flavones rapidly and efficiently.And it is logical
Crossing literature search, the domestic Senecio dianthus total flavones that there is not yet is for preparing anti-oxidation medicine.
Summary of the invention
It is an object of the invention to seek the exploitation of the new and effective Natural antioxidant, it is provided that a kind of Flos Lonicerae thousand
In the preparation method and applications of light total flavones.
For achieving the above object, technical scheme is as follows:
A. by Senecio dianthus total flavones in 60 DEG C of drying, coarse powder is obtained；
B. being placed in microwave-Vltrasonic device by Senecio dianthus powder, the ethanol of the 70 ~ 80% of addition raw material weight 3 ~ 5 times is molten
Liquid, arranges that ultrasonic 500W is normally opened, microwave power is 600 ~ 800W, extracts 2 ~ 3h, filters, and filtrate reduced in volume obtains concentrated solution；
C. concentrated solution is joined macroporous adsorptive resins, first with the distilled water eluting of 2 ~ 3BV, then with the 65 ~ 75% of 4 ~ 6BV
Ethanol elution, collects eluent；
D. eluent concentrates, and is dried, obtains Senecio dianthus total flavones.
Macroporous adsorbent resin type described in step c is the one in AB-8, HPD300, XDA-1.
Senecio dianthus total flavones prepared by the present invention application in preparation has the medicine of antioxidation.
The Senecio dianthus total flavones that the present invention obtains, by its anti peroxidation of lipid ability, removing superoxide anion
Free radical (-O2)2The research of the index such as ability and scavenging ability of DPPH free radical shows: Senecio dianthus total flavones has the strongest
Anti peroxidation of lipid ability, simultaneously can also effectively remove ultra-oxygen anion free radical and DPPH free radical.Therefore, Flos Lonicerae thousand
In light total flavones to be that of the new and effective Natural antioxidant of exploitation is good select.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to
In following embodiments.
Detailed description of the invention
Embodiment 1:
By Senecio dianthus total flavones in 60 DEG C of drying, obtain coarse powder；1000g Senecio dianthus powder is placed in microwave-ultrasonic dress
In putting, add 4L 80% ethanol solution, arrange that ultrasonic 500W is normally opened, microwave power is 800W, extract 3h, filter, filtrate subtracts
Pressure is concentrated to give concentrated solution；Concentrated solution is joined HPD300 macroporous adsorptive resins, first with the distilled water eluting of 2 times of column volumes,
Again with the 75% of 6 times of column volumes ethanol elution, collect eluent；Eluent concentrates, and is dried, obtains Senecio dianthus total flavones
110.1g.Detecting through UV, the content of Senecio dianthus total flavones is 78.9%.
Embodiment 2:
By Senecio dianthus total flavones in 60 DEG C of drying, obtain coarse powder；1000g Senecio dianthus powder is placed in microwave-ultrasonic dress
In putting, add 3L 70% ethanol solution, arrange that ultrasonic 500W is normally opened, microwave power is 600W, extract 2h, filter, filtrate subtracts
Pressure is concentrated to give concentrated solution；Concentrated solution is joined AB-8 macroporous adsorptive resins, first with the distilled water eluting of 2 times of column volumes, then
With the ethanol elution of the 65% of 4 times of column volumes, collect eluent；Eluent concentrates, and is dried, obtains Senecio dianthus total flavones
114.8g.Detecting through UV, the content of Senecio dianthus total flavones is 81.2%.
Embodiment 3:
By Senecio dianthus total flavones in 60 DEG C of drying, obtain coarse powder；1000g Senecio dianthus powder is placed in microwave-ultrasonic dress
In putting, add 5L 75% ethanol solution, arrange that ultrasonic 500W is normally opened, microwave power is 600W, extract 2.5h, filter, filtrate
Concentrating under reduced pressure obtains concentrated solution；Concentrated solution is joined XDA-1 macroporous adsorptive resins, first with the distilled water eluting of 3 times of column volumes,
Again with the 70% of 5 times of column volumes ethanol elution, collect eluent；Eluent concentrates, and is dried, obtains Senecio dianthus total flavones
113.2g.Detecting through UV, the content of Senecio dianthus total flavones is 83.6%.
Concrete pharmacodynamic study experiment:
By the Senecio dianthus total flavones of gained in above-mentioned for 1g 3 embodiments, dissolved constant volume with the ethanol of 30% respectively and existed
In 50mL volumetric flask, it is designated as A, B, C, standby.
A. the mensuration of anti peroxidation of lipid ability
Be sequentially added in sample cell 1mL 10mg/mL lecithin soln (LLS), 1mL 0.4mmol/L ferrous sulfate,
Tri-samples of 1mL above-mentioned A, B, C, mixing, lucifuge, in 37 DEG C of water-bath 60min, adds 2mL trichloroacetic acid (TCA)-sulfur for barbital
Acid (TBA)-HCl mixed liquor, 90 DEG C ~ 100 DEG C water-bath 15min, cool down rapidly, be centrifuged 10min with 3000r/min, take supernatant
Survey absorbance (As) at 535nm, reference tube replaces 1mL lecithin with 1mL distilled water.Ascorbic acid (VC) is positive control.
The results are shown in Table 1.
Suppression ratio=[Ac-As/ Ac] × 100%
In formula: Ac is the absorbance of reference substance (replacing sample with distilled water)；
Absorbance when As is to add tri-samples of A, B, C.
Table 1 Senecio dianthus total flavones anti peroxidation of lipid ability mensuration (± S)
| Sample | Suppression ratio (%) |
| A | 30.8 |
| B | 41.6 |
| C | 43.4 |
| VC(1mg/mL) | 16.1 |
| VC(2mg/mL) | 42.9 |
Senecio dianthus total flavones is to Fe2+The lecithin liposome peroxidating caused has obvious inhibitory action, and suppression ratio is with sample
The increase of general flavone content in product and increase.As can be seen from Table 1, Senecio dianthus total flavones anti peroxidation of lipid ability is with anti-
Bad hematic acid is suitable.
B. remove ultra-oxygen anion free radical (-O2)2The mensuration of ability
Use assay NBT photoreduction, take 50mmol/L Tris-HCl buffer solution (pH=8.2) 4.5mL, be placed in 25 DEG C of water
Bath preheats 20min, is separately added into 1mL sample and 0.4mL 25mmol/L pyrogallol solution, after mixing in 25 DEG C of water-baths
After reaction 5min, add 8mol/L HCl 0.8mL and terminate reaction.Autoxidation pipe replaces sample in sample cell with 1mL distilled water,
The same sample cell of operational approach, measures light absorption value, with the Tris-HCl buffer of equal-volume pH value 8.2 as blank at 325nm.Knot
Fruit is shown in Table 2:
The clearance rate of superoxide anion=[A0-A1/ A0] ×100%
In formula: A0Absorbance for autoxidation pipe；
A1Absorbance for sample cell.
Table 2 Senecio dianthus total flavones removing ultra-oxygen anion free radical ability mensuration (± S)
| Sample | Suppression ratio (%) |
| A | 74.14 |
| B | 78.12 |
| C | 86.45 |
| VC(1mg/mL) | 69.34 |
| VC(2mg/mL) | 88.45 |
Senecio dianthus total flavones has preferable elimination effect to removing ultra-oxygen anion free radical, and clearance rate is with total yellow in sample
The increase of ketone content and increase.As can be seen from Table 2, Senecio dianthus total flavones is outstanding to the clearance rate of ultra-oxygen anion free radical
For significantly, hence it is evident that being better than Vc(when concentration is 1mg/mL, clearance rate is 69.34%).
C. the scavenging action to DPPH free radical
In test tube, it is separately added into the european bird cherry leaf flavonoids solution of each concentration of 2.00mL, numbered A, B, C, adds
2.00mL0.2mml/LDPPH ethanol solution, shakes up, and places 30min, replaces sample and 2.00mL70% with 2.00mL dehydrated alcohol
As reference after ethanol mixing, measure the absorbance (A at 517nm wavelengthi).Blank group replaces with 2.00mL dehydrated alcohol
Sample, measures the absorbance (A at 517nm wavelength0), measure 2.00mL liquid to be measured with 2.00mL dehydrated alcohol at 517nm simultaneously
Absorbance (A at wavelengthj).With Vc as reference substance.The results are shown in Table 3:
DPPH free radical scavenging activity clicks formula and calculates:
Clearance rate=[A0-(Ai-Aj)]/A0×100%
In formula: A0It is not added with the light absorption value of flavone, AiMeasure the light absorption value of liquid, AjThe background light absorption value of flavone.
Table 3 Senecio dianthus total flavones to DPHH free radical ability measure (± S)
| Sample | Clearance rate (%) |
| A | 67 |
| B | 74 |
| C | 82 |
| VC(1mg/mL) | 86 |
| VC(2mg/mL) | 85 |
From table 3 it is observed that along with the increase of content, Senecio dianthus total flavones removes the ability of DPPH free radical along with dense
The increase of degree and increase, have an obvious dose dependent, experimental result explanation Senecio dianthus total flavones is that good proton supplies
Body, has significant antioxidation.
Claims (3)
1. the preparation method of a Senecio dianthus total flavones, it is characterised in that comprise the following steps:
A. by Senecio dianthus total flavones in 60 DEG C of drying, coarse powder is obtained；
B. being placed in microwave-Vltrasonic device by Senecio dianthus powder, the ethanol of the 70 ~ 80% of addition raw material weight 3 ~ 5 times is molten
Liquid, arranges that ultrasonic 500W is normally opened, microwave power is 600 ~ 800W, extracts 2 ~ 3h, filters, and filtrate reduced in volume obtains concentrated solution；
C. concentrated solution is joined macroporous adsorptive resins, first with the distilled water eluting of 2 ~ 3BV, then with the 65 ~ 75% of 4 ~ 6BV
Ethanol elution, collects eluent；
D. eluent concentrates, and is dried, obtains Senecio dianthus total flavones.
The preparation method of a kind of Senecio dianthus total flavones the most according to claim 1, it is characterised in that institute in step c
The macroporous adsorbent resin type stated is the one in AB-8, HPD300, XDA-1.
Senecio dianthus total flavones prepared by method the most according to claim 1 or claim 2 has the medicine of antioxidation in preparation
Application in thing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109248184A (en) * | 2018-11-06 | 2019-01-22 | 贵州工程应用技术学院 | The extracting method of total flavonoids substance in a kind of novel climbing groundsel |
| CN112716991A (en) * | 2021-01-29 | 2021-04-30 | 西藏天虹科技股份有限责任公司 | Method for extracting and purifying total flavonoids of senecio dianthus |
-
2016
- 2016-05-11 CN CN201610306676.2A patent/CN106038644A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109248184A (en) * | 2018-11-06 | 2019-01-22 | 贵州工程应用技术学院 | The extracting method of total flavonoids substance in a kind of novel climbing groundsel |
| CN112716991A (en) * | 2021-01-29 | 2021-04-30 | 西藏天虹科技股份有限责任公司 | Method for extracting and purifying total flavonoids of senecio dianthus |
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Application publication date: 20161026 |