CN106018365A - Kaempferol and cyclodextrin compound liquid and application thereof - Google Patents
Kaempferol and cyclodextrin compound liquid and application thereof Download PDFInfo
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- CN106018365A CN106018365A CN201610335343.2A CN201610335343A CN106018365A CN 106018365 A CN106018365 A CN 106018365A CN 201610335343 A CN201610335343 A CN 201610335343A CN 106018365 A CN106018365 A CN 106018365A
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- kaempferol
- cyclodextrin
- copper ion
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- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 title claims abstract description 96
- 229920000858 Cyclodextrin Polymers 0.000 title claims abstract description 57
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 title claims abstract description 48
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 title claims abstract description 48
- 235000008777 kaempferol Nutrition 0.000 title claims abstract description 48
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 title claims abstract description 48
- 239000007788 liquid Substances 0.000 title claims abstract description 12
- -1 cyclodextrin compound Chemical class 0.000 title abstract description 5
- 239000000243 solution Substances 0.000 claims abstract description 36
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 229910002056 binary alloy Inorganic materials 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 11
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 claims description 49
- 229910001431 copper ion Inorganic materials 0.000 claims description 48
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
- 239000002953 phosphate buffered saline Substances 0.000 claims description 16
- 239000007853 buffer solution Substances 0.000 claims description 13
- 230000001419 dependent effect Effects 0.000 claims description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 3
- 230000005284 excitation Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 239000000523 sample Substances 0.000 abstract description 6
- 150000002500 ions Chemical class 0.000 abstract description 5
- 229910021645 metal ion Inorganic materials 0.000 abstract description 4
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 230000035945 sensitivity Effects 0.000 abstract 1
- ODLHGICHYURWBS-LKONHMLTSA-N trappsol cyclo Chemical compound CC(O)COC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)COCC(O)C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1COCC(C)O ODLHGICHYURWBS-LKONHMLTSA-N 0.000 abstract 1
- 239000010949 copper Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 7
- 229910052802 copper Inorganic materials 0.000 description 7
- 230000003139 buffering effect Effects 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 238000000954 titration curve Methods 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000218628 Ginkgo Species 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 208000027089 Parkinsonian disease Diseases 0.000 description 1
- 206010034010 Parkinsonism Diseases 0.000 description 1
- 241001062472 Stokellia anisodon Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 206010000059 abdominal discomfort Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000010793 electronic waste Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 238000007421 fluorometric assay Methods 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000000918 plasma mass spectrometry Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6423—Spectral mapping, video display
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- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Optics & Photonics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses kaempferol and cyclodextrin compound liquid and an application thereof. The kaempferol and cyclodextrin compound liquid is binary compound liquid of the kaempferol and the cyclodextrin, wherein the concentration of the kaempferol is 1x10-5 mol-L-1; the cyclodextrin is 2-hydroxypropyl-beta-cyclodextrin; and the quality concentration ratio of the cyclodextrin to the kaempferol is 100-800:1. The kaempferol and cyclodextrin binary system compound liquid is used as a fluorescence probe to detect cupric ions in an aqueous solution, is quick and simple to operate and high in recognition capability to the cupric ions, is seldom disturbed by other metal ions, and is high in sensitivity, large in linearity range and low in limit of detection. Compared with independent kaempferol, the cyclodextrin can remarkably enhance fluorescence intensity of the kaempferol, the fluorescence intensity of a kaempferol solution is gradually reduced along with time passing, but the fluorescence intensity of a kaempferol-cyclodextrin binary system is hardly changed along with time passing, and stability is greatly improved.
Description
Technical field
The invention belongs to spectrum analysis detection technique field, be specifically related to the compositional liquor of kaempferol and cyclodextrin and answer
With.
Background technology
Copper is widely present in the middle of nature and human body, is the indispensable trace element of human body, meanwhile,
Copper is widely used in the industries closely bound up with human lives such as metal smelt, electronics, packaging, along with people
Class social development speed is accelerated, and mineral resources disorderly exploitation phenomenon is day by day serious, except three industrial wastes, house refuse,
Electronic waste is the most increasing, ultimately results in heavy metal ion and runs off, causes soil, Heavy Metals in Waters ion
Content overproof.Although the copper requisite trace element that is human body, but, copper too high levels, gently
Person causes gastrointestinal upset, and severe one can cause nervous lesion, parkinsonism, even life-threatening, therefore,
In monitoring of environmental, the content of copper ion seems critically important.
At present, the method for detection copper ion is a lot, common are: inductivity coupled plasma mass spectrometry, flame are former
Sub-absorption process, electrochemical process etc., but these methods will be used many precision instruments, and locate before sample
Reason requires strict, and testing cost is high, inefficient.For overcoming disadvantage mentioned above, use Fluorometric assay copper ion
Report get more and more.Fluorescence method, in addition to having highly sensitive, good selective, also has detection
Quickly, easy, low cost and other advantages.But, most fluorescent probes being used for detecting copper ion are to pass through
Organic synthesis approach obtains, and uses many toxic chemical, virtually cause the two of environment in experimentation
Secondary pollution.
Kaempferol (C15H10O6, kaempferol) and it is flavone compound, it is widely present in nature
In the plant on boundary, such as: Folium Ginkgo, Folium Nelumbinis, Bulbus Allii Cepae etc..It is multiple that kaempferol has antioxidation, antiinflammatory etc.
Biological activity, but its fluorescence property relevant research is less.
Summary of the invention
Goal of the invention: for the deficiencies in the prior art, it is an object of the present invention to provide kaempferol and cyclodextrin
Compositional liquor, meet and use measuring in copper ion.It is a further object of the present invention to provide above-mentioned kaempferol and ring
The application of the compositional liquor of dextrin, has that selectivity is good, highly sensitive, the range of linearity is wide, simple and environmentally-friendly low toxicity
Etc. feature.
Technical scheme: in order to realize foregoing invention purpose, the technical solution used in the present invention is:
The application in measuring copper ion of the compositional liquor of kaempferol and cyclodextrin.
Described application, comprises the following steps:
(1) preparation kaempferol and cyclodextrin binary system buffer solution；
(2) buffering that the copper ion solution of different volumes joins kaempferol-cyclodextrin binary system respectively is molten
In liquid, use the fluorescence intensity of each solution of fluorescence spectrophotometer record, draw the fluorescence intensity mark to copper ion concentration
Directrix curve；
(3) liquid to be measured for copper ion is joined in kaempferol-cyclodextrin binary system solution, use fluorescence spectrum
The fluorescence intensity of instrument recording solution；
(4) concentration of copper ion in solution to be measured is determined according to standard curve.
Described buffer solution is CH3OH-PBS buffer solution, the pH of solution is 7.4, and methanol delays with phosphoric acid
The volume ratio rushing saline solution is 1～5:99.
Described fluorescent strength determining condition is that to excite slit be 5nm, and transmitting slit is 15nm, sweep limits
For 400-700nm, excitation wavelength is 390nm, a length of 552nm of transmitted wave.
The concentration of described kaempferol is 1 × 10-5mol·L-1, cyclodextrin is 2-HP-BETA-CD, and ring is stuck with paste
Essence is 100～800:1 with the mass concentration ratio of kaempferol.
Described copper ion linear concentration scope is 5.0 × 10-8-5.0×10-6mol·L-1, detection is limited to 1.5 × 1
0-8mol·L-1。
Described application, the fluorescence intensity standard curve to copper ion concentration, y=-8.54x+708.55, y
For fluorescence intensity, x is copper ion concentration, and linearly dependent coefficient is R2=0.997.
A kind of compositional liquor for detecting copper ion, for the binary built liquid of kaempferol Yu cyclodextrin, wherein, mountain
How the concentration of phenol is 1 × 10-5mol·L-1, cyclodextrin is 2-HP-BETA-CD, cyclodextrin and kaempferol
Mass concentration ratio is 100～800:1.
The described compositional liquor for detecting copper ion, solvent is CH3OH-PBS buffer solution, the p of solution
H is 7.4, and methanol is 1～5:99 with the volume ratio of phosphate buffered saline(PBS).
Beneficial effect: compared with prior art, the present invention utilizes kaempferol-cyclodextrin binary system compositional liquor to make
The copper ion in aqueous solution is detected, kaempferol-cyclodextrin binary before and after utilizing copper ion to add for fluorescent probe
The Changing Pattern of system fluorescence intensity, draws the fluorescence intensity standard curve to copper ion concentration, bent according to standard
The concentration of copper ion in unknown sample obtained by line.Simple to operate, fast, strong to the identification ability of copper ion, it is subject to
Other metal ion disturbance is little, highly sensitive, and the range of linearity is big, and detection limit is low.Be used alone kaempferol phase
Ratio, cyclodextrin can be obviously enhanced the fluorescence intensity of kaempferol, and the fluorescence intensity of kaempferol solution increases over time
Add and be gradually lowered, and the fluorescence intensity of kaempferol-cyclodextrin binary system changes over not quite, stability
It is greatly improved.
Accompanying drawing explanation
Fig. 1 is the cyclodextrin potentiation figure to kaempferol fluorescence intensity；
Fig. 2 is kaempferol and kaempferol-cyclodextrin binary system fluorescence intensity variation diagram over time；
Fig. 3 is the kaempferol-cyclodextrin binary system fluorescence titration spectrogram to copper ion；
Fig. 4 is the kaempferol-cyclodextrin binary system fluorescence titration curve chart to copper ion；
Fig. 5 is the kaempferol-cyclodextrin binary system selectivity figure to different metal ion.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1
Kaempferol, kaempferol and the compositional liquor of cyclodextrin, the Application comparison in measuring copper ion, step is as follows:
(1) accurately weigh 0.0029g kaempferol, with in methanol constant volume to 10mL volumetric flask, be made into concentration
It is 1 × 10-3mol·L-1Solution；Accurately weigh 0.7250g 2-HP-BETA-CD, molten by PBS buffering
Liquid (pH=7.40) is settled in 25mL volumetric flask.
(2) take 1.0mL kaempferol solution, be settled to 100 with PBS buffer solution (pH=7.40)
In mL volumetric flask, use fluorescence spectrophotometer fluorescence intensity, survey first order fluorescence intensity every 1h.
(3) take 6.0mL cyclodextrin solution, be settled to 100 with PBS buffer solution (pH=7.40)
In mL volumetric flask, use fluorescence spectrophotometer fluorescence intensity.
(4) 1.0mL kaempferol solution and 6.0mL cyclodextrin solution are taken respectively, with PBS buffer solution (p
H=7.40) it is settled in 100mL volumetric flask, is made into kaempferol-cyclodextrin binary system, and uses glimmering
Photothermal spectroscopic analyzer fluorescence intensity, surveys first order fluorescence intensity every 1h.
Cyclodextrin to kaempferol Fluorescence Enhancement as it is shown in figure 1, cyclodextrin can be obviously enhanced the fluorescence of kaempferol
Intensity.The fluorescence intensity of kaempferol and kaempferol-cyclodextrin binary system over time as in figure 2 it is shown,
The fluorescence intensity of kaempferol solution increases over time and is gradually lowered, and kaempferol-cyclodextrin binary system
Fluorescence intensity changes over not quite, and stability is greatly improved.
Embodiment 2
The application in measuring copper ion of the compositional liquor of kaempferol and cyclodextrin, specifically includes following steps:
(1) accurately weigh 0.0029g kaempferol, with in methanol constant volume to 10mL volumetric flask, be made into concentration
It is 1 × 10-3mol·L-1Solution；Accurately weigh 0.7250g 2-HP-BETA-CD, molten by PBS buffering
Liquid (pH=7.40) is settled in 25mL volumetric flask.Take 1.0mL kaempferol solution and 6.0mL respectively
Cyclodextrin solution, is settled in 100mL volumetric flask with PBS buffer solution (pH=7.40), is made into mountain
How phenol-cyclodextrin binary system, stand-by.
(2) 0.0200g Cu (AC) is accurately weighed2·H2O water is settled in 10mL volumetric flask, is made into and contains
Having copper ion concentration is 1 × 10-2mol·L-1Solution, and be diluted to 1 × 10 successively-3, 1 × 10-4, 1 × 10-5mol·L-1Solution, stand-by.
(3) prepare the sample cell of some dried and clean, be separately added into kaempferol-cyclodextrin binary system solution
With the copper ion solution of different volumes variable concentrations, it is made into kaempferol concentration and is 1 × 10-5mol·L-1, copper from
Sub-concentration is respectively 0 × 10-7mol·L-1, 0.5 × 10-7mol·L-1, 1.0 × 10-7mol·L-1, 3.0 × 10-7mol·L-1, 5.0 × 10-7mol·L-1, 7.0 × 10-7mol·L-1, 10.0 × 10-7mol·L-1, 13.0 ×
10-7mol·L-1, 15.0 × 10-7mol·L-1, 17.0 × 10-7mol·L-1, 20.0 × 10-7mol·L-1, 23.
0×10-7mol·L-1, 25.0 × 10-7mol·L-1, 27.0 × 10-7mol·L-1, 30.0 × 10-7mol·L-1,
33.0×10-7mol·L-1, 35.0 × 10-7mol·L-1, 37.0 × 10-7mol·L-1, 40.0 × 10-7mol·L- 1, 43.0 × 10-7mol·L-1, 45.0 × 10-7mol·L-1, 47.0 × 10-7mol·L-1, 50.0 × 10-7mol·L-1Solution.
(4) arranging fluorescence spectrophotometer excitation wavelength is 390nm, excites and launches slit width and is respectively 5nm
And 15nm, sweep limits is set to 400nm to 700nm；Above-mentioned solution is tested, respectively on record
State solution fluorescence intensity at a length of 552nm of transmitted wave, result as it is shown on figure 3, from 1 to 23, copper from
Sub-concentration is followed successively by 0 × 10-7mol·L-1, 0.5 × 10-7mol·L-1, 1.0 × 10-7mol·L-1, 3.0 × 10-7mol·L-1, 5.0 × 10-7mol·L-1, 7.0 × 10-7mol·L-1, 10.0 × 10-7mol·L-1, 13.0 ×
10-7mol·L-1, 15.0 × 10-7mol·L-1, 17.0 × 10-7mol·L-1, 20.0 × 10-7mol·L-1, 23.
0×10-7mol·L-1, 25.0 × 10-7mol·L-1, 27.0 × 10-7mol·L-1, 30.0 × 10-7mol·L-1,
33.0×10-7mol·L-1, 35.0 × 10-7mol·L-1, 37.0 × 10-7mol·L-1, 40.0 × 10-7mol·L- 1, 43.0 × 10-7mol·L-1, 45.0 × 10-7mol·L-1, 47.0 × 10-7mol·L-1, 50.0 × 10-7mol·L-1。
(5) fluorescence intensity standard curve to copper ion concentration is drawn, as shown in Figure 4.Gained linear equation
For: y=-8.54x+708.55, y are fluorescence intensity, and x is copper ion concentration, and linearly dependent coefficient is R2
=0.997, the range of linearity is 5.0 × 10-8-5.0×10-6mol·L-1, detection is limited to 1.5 × 10-8mol·L-1, can
To complete the detection to trace copper ion.
(6) mensuration of sample: configure standard specimen according to the method described above and treat test sample (unknown concentration), mountain in solution
How phenol concentration is 1 × 10-5mol·L-1, in standard specimen, copper ion concentration is 2.0 × 10-7mol·L-1(standard specimen 1),
22.0×10-7mol·L-1(standard specimen 2), 32.0 × 10-7mol·L-1(standard specimen 3)；Use same procedure to above-mentioned
Solution carries out fluorometric investigation, records above-mentioned solution fluorescence intensity at a length of 552nm of transmitted wave, repeats to survey
Three times, according to the fluorescence intensity standard curve y=-8.54x+708.55 to copper ion concentration, it is calculated copper
The concentration of ion, concrete outcome is as shown in table 1.
Table 1 measurement result table.
In table, concentration unit is 10-7mol·L-1。
Embodiment 3
Containing in kaempferol-cyclodextrin binary system, it is separately added into containing Li+、Na+、K+、Mg2+、Ca2+、
Al3+、Cr3+、Mn2+、Fe2+、Fe3+、Co2+、Ni2+、Cu2+、Zn2+、Pb2+、Cd2+、La3+、Ce3+、Nd3+、Eu3+、Gd3+、Dy3+Saline solution, wherein Li+、Na+、K+、Mg2+、Ca2+Concentration
For 5 times of copper ion concentration, Al3+、Cr3+、Mn2+、Fe2+、Fe3+、Co2+、Ni2+、Zn2+、Pb2+、
Cd2+、La3+、Ce3+、Eu3+、Gd3+、Dy3+Concentration is 2 times of copper ion concentration, in embodiment 2 phase
The fluorescence intensity recorded under same condition and method is as shown in Figure 5.As seen from Figure 5, only copper ion
Adding membership causes kaempferol-cyclodextrin binary system to produce obvious fluorescent quenching, and other metal ion is to Rhizoma Kaempferiae
The impact of phenol-cyclodextrin binary system fluorescence intensity is the least, can ignore, and demonstrates kaempferol-cyclodextrin binary
System has preferable selectivity to copper ion.Additionally, from fluorescence titration spectrum and titration curve it can be seen that
The range of linearity of kaempferol-cyclodextrin binary system detection copper ion is big, and detection limit is relatively low, demonstrate kaempferol-
Cyclodextrin binary system has preferable susceptiveness to copper ion.
As can be seen here, the method using the present invention, kaempferol-cyclodextrin binary system can be as fluorescent probe
The copper ion of trace is detected, not only there is high selectivity and susceptiveness, and fast, be environment-friendly and low-toxicity,
Result is preferable.
Claims (9)
1. the compositional liquor of kaempferol and cyclodextrin application in measuring copper ion.
Application the most according to claim 1, it is characterised in that comprise the following steps:
(1) preparation kaempferol and cyclodextrin binary system buffer solution；
(2) respectively the copper ion solution of different volumes is joined in the buffer solution of kaempferol-cyclodextrin binary system, use the fluorescence intensity of each solution of fluorescence spectrophotometer record, draw the fluorescence intensity standard curve to copper ion concentration；
(3) liquid to be measured for copper ion is joined in kaempferol-cyclodextrin binary system solution, use the fluorescence intensity of fluorescence spectrophotometer recording solution；
(4) concentration of copper ion in solution to be measured is determined according to standard curve.
Application the most according to claim 2, it is characterised in that: described buffer solution is CH3OH-PBS buffer solution, the pH of solution is 7.4, and methanol is 1 ~ 5:99 with the volume ratio of phosphate buffered saline(PBS).
Application the most according to claim 2, it is characterised in that: described fluorescent strength determining condition is that to excite slit be 5nm, and transmitting slit is 15nm, and sweep limits is 400-700nm, and excitation wavelength is 390nm, a length of 552nm of transmitted wave.
Application the most according to claim 2, it is characterised in that: the concentration of described kaempferol is 1 × 10-5mol•L-1, cyclodextrin is 2-HP-BETA-CD, and cyclodextrin is 100 ~ 800:1 with the mass concentration ratio of kaempferol.
Application the most according to claim 2, it is characterised in that: described copper ion linear concentration scope is 5.0 × 10-8-5.0×10-6 mol•L-1, detection is limited to 1.5 × 10-8 mol•L-1。
Application the most according to claim 2, it is characterised in that: the fluorescence intensity standard curve to copper ion concentration,y = -8.54x+ 708.55,yFor fluorescence intensity,xFor copper ion concentration, linearly dependent coefficient is R2=0.997。
8. the compositional liquor being used for detecting copper ion, it is characterised in that for the binary built liquid of kaempferol Yu cyclodextrin, wherein, the concentration of kaempferol is 1 × 10-5mol•L-1, cyclodextrin is 2-HP-BETA-CD, and cyclodextrin is 100 ~ 800:1 with the mass concentration ratio of kaempferol.
Compositional liquor for detecting copper ion the most according to claim 8, it is characterised in that the solvent of compositional liquor is CH3OH-PBS buffer solution, the pH of solution is 7.4, and methanol is 1 ~ 5:99 with the volume ratio of phosphate buffered saline(PBS).
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608092A (en) * | 2012-03-28 | 2012-07-25 | 哈尔滨工业大学 | Fluorescence biosensor for detecting high-sensitivity copper ionss and detection method thereof |
| CN103344588A (en) * | 2013-06-28 | 2013-10-09 | 上海理工大学 | Method for detecting trace concentration of copper ions |
| CN104007092A (en) * | 2014-05-19 | 2014-08-27 | 中国科学院长春应用化学研究所 | Fluorescence detection method for copper ion based on click chemistry |
| WO2014200164A1 (en) * | 2013-06-11 | 2014-12-18 | 한국기초과학지원연구원 | Composition having ultra-high sensitivity and high selectivity for detecting copper ion and fluorescent chemical sensor |
| CN104569122A (en) * | 2015-01-22 | 2015-04-29 | 广西师范学院 | Method for detecting concentration of copper ions in solution |
-
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608092A (en) * | 2012-03-28 | 2012-07-25 | 哈尔滨工业大学 | Fluorescence biosensor for detecting high-sensitivity copper ionss and detection method thereof |
| WO2014200164A1 (en) * | 2013-06-11 | 2014-12-18 | 한국기초과학지원연구원 | Composition having ultra-high sensitivity and high selectivity for detecting copper ion and fluorescent chemical sensor |
| CN103344588A (en) * | 2013-06-28 | 2013-10-09 | 上海理工大学 | Method for detecting trace concentration of copper ions |
| CN104007092A (en) * | 2014-05-19 | 2014-08-27 | 中国科学院长春应用化学研究所 | Fluorescence detection method for copper ion based on click chemistry |
| CN104569122A (en) * | 2015-01-22 | 2015-04-29 | 广西师范学院 | Method for detecting concentration of copper ions in solution |
Non-Patent Citations (1)
| Title |
|---|
| HISTIDINEISMAIL ABULKALAM AZATH,ET AL.: "Flavone modified-β-cyclodextrin as a highly selective and efficient fluorescent chemosensor for Cu2+ ions and L-histidine", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
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