CN106010926A - Eucommia bark fermented health care vinegar and preparation method thereof - Google Patents
Eucommia bark fermented health care vinegar and preparation method thereof Download PDFInfo
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- CN106010926A CN106010926A CN201610375405.2A CN201610375405A CN106010926A CN 106010926 A CN106010926 A CN 106010926A CN 201610375405 A CN201610375405 A CN 201610375405A CN 106010926 A CN106010926 A CN 106010926A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12J—VINEGAR; PREPARATION OR PURIFICATION THEREOF
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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Abstract
The invention discloses eucommia bark fermented health care vinegar and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a basic fermentation solution: crushing ponkan pulp, extracting juice, conducting coarse filtration, conveying the obtained juice into a cloudy juice tank, adding a folium cortex eucommiae extracting solution, regulating a sugar content and conducting pasteurization, so that the basic fermentation solution is prepared; (2) conducting primary fermentation: inoculating the basic solution with an activated wine-activating yeast, and conducting fermentation at 20-28 DEG C for 5-10 days, so that eucommia bark-ponkan fruit wine is prepared, conducting filtration, and conveying the fruit wine into an acetic acid fermentation tank; and (3) conducting secondary fermentation: adding 8-12% of domesticated acetic acid bacterium liquid to the eucommia bark-ponkan fruit wine; and conducting aerating agitation for 2-3 times every day at 26-33 DEG C for 3-4 days, 1min per time, so that the fruit wine gets into full contact with oxygen, and subsequently, the health care vinegar is obtained. According to the health care vinegar and the preparation method thereof disclosed by the invention, by effectively integrating such technologies as vacuum pulsation enhanced mass transfer, biological fermentation, enzymatic hydrolysis and the like, an optimum effect of preparing the eucommia bark fermented health care vinegar from the folium cortex eucommiae and the ponkan juice is achieved; and the health care vinegar is strong in market competitiveness and broad in application prospect.
Description
Technical field
The present invention relates to a kind of Cortex Eucommiae fermentation health preserving vinegar and preparation method thereof, refer specifically to Folium Eucommiae extracting solution and the method for Citrus chachiensis Hort. juice ferment making fruit vinegar.
Background technology
Folium Eucommiae is the dried leaves of the Eucommiaceae plant Cortex Eucommiae, the summer, season in autumn two branch and leaf luxuriant time gather, dry or oven drying at low temperature.Modern pharmacology research proves: Folium Eucommiae and leatherware have intimate pharmacological action, have invigorating the liver and kidney, and the effect of bone and muscle strengthening, for deficiency of the liver and kindey, is had a dizzy spell, and soreness of waist and knee joint, muscles and bones flaccidity is soft, and hyperlipidemia, hypertension etc..
Citrus chachiensis Hort. vinegar is a kind of fermented type fruit vinegar made for raw material with Citrus chachiensis Hort. juice, and modern medicine study shows: fruit vinegar can enhance metabolism, and regulates acid-base balance, and allaying tiredness, containing organic acid and the several amino acids of needed by human body of more than ten kinds in fruit vinegar.
Ferment health preserving vinegar with Citrus chachiensis Hort. and Folium Eucommiae for the main material production Cortex Eucommiae, the added value of Citrus chachiensis Hort. and Folium Eucommiae can be promoted, the kind of abundant vinegar, expand Citrus chachiensis Hort. and the industry bore of Folium Eucommiae, it can be also the health promoting vinegar product of human health offer diversification, Folium Eucommiae is added, moreover it is possible to obtain beyond thought health care and therapeutic effect in the production process of fruit vinegar.
But the most to greatest extent the effective ingredient in Folium Eucommiae is incorporated in vinegar product, dissolve the inhibitory action of antimicrobial component Dichlorodiphenyl Acetate bacterium in Folium Eucommiae, and how to remove the bitter taste of Folium Eucommiae itself, the mouthfeel of improvement vinegar product and improve the market adaptability of product, it is the technical barrier making Cortex Eucommiae fermentation health preserving vinegar, is also not solve the technical problem that present stage.
Summary of the invention
For solving the problem that above-mentioned prior art exists, the present inventor through substantial amounts of test, grope and sum up a kind of with Folium Eucommiae and Citrus chachiensis Hort. Cortex Eucommiae fermentation health preserving vinegar as primary raw material and preparation method thereof.
The present invention includes following step:
(1) fermenation raw liquid makes: by hesperidium aurantium meat broken juice, coarse filtration, entrance turbid juice tank, add the Folium Eucommiae extracting solution of Citrus chachiensis Hort. juice volume 10%-20%, then the sugar content adjusting Citrus chachiensis Hort. juice is 15-16%, at 75 DEG C, pasteurization under conditions of 15 minutes, make fermenation raw liquid, be delivered to ethanol fermentation tank.
The preparation method of described Folium Eucommiae extracting solution is: toward being broken in the dry Folium Eucommiae of 20-40 purpose the enzymolysis solution adding 6-10 times amount, enzymolysis 1-4 hour at a temperature of 40-50 degree, after enzymolysis completes, the edible ethanol of addition 95% is until the volume fraction of ethanol is 20%-30%, carry out reflux, extract, 2-6 hour extraction time, after having extracted, it is evaporated to produce precipitate, standby.Enzymolysis solution is with the volume mass ratio of dry Folium Eucommiae, and the unit of volume is milliliter, and the unit of quality is gram.
Enzymolysis solution preparation method is: is dissolved in the buffer that 500ml pH value is 4-7 by 1-15 gram of compound enzymic preparation and get final product.The proportioning of compound enzymic preparation is cellulase mass fraction 60-70%, pectase mass fraction is 15-25%, remaining is xylanase, the average enzyme of compound enzymic preparation is lived as 20000-30000 unit, described enzyme unit alive defers to international zymetology meeting in 1961 regulation, refers under specified conditions (25 DEG C, other is optimal condition), the enzyme amount of 1 micromole substrate, or the 1 micromolar enzyme amount about group in conversion of substrate can be converted in 1 minute.Tests prove that, with the extraction ratio that can significantly improve the effective ingredient such as Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves, aucubin after ferment treatment.
Extraction pot can be carried out step evacuation and vacuum breaker during said extracted, utilize vacuum impulse to strengthen mass transfer.The mechanism of vacuum impulse strengthening mass transfer is: owing to ethanol is the organic solvent that volatility is stronger, during evacuation, the ethanol in Folium Eucommiae cell tissue can gasify because of decompression, in causing Folium Eucommiae cell tissue, pressure is more than outside Folium Eucommiae histiocyte, and the ethanol having dissolved effective ingredient can be forced through Folium Eucommiae cell tissue under differential pressure action;When vacuum breaker, Folium Eucommiae histiocyte external pressure is intrinsic pressure more than Folium Eucommiae histiocyte, ethanol outside Folium Eucommiae histiocyte quickly enters because of differential pressure action and goes inside Folium Eucommiae histiocyte to dissolve effective ingredient, the alternate of this Folium Eucommiae histiocyte external and internal pressure enhances ethanol and dissolves effective ingredient mass transfer in ethanol, makes the effective ingredient residual rates such as extraction efficiency raising, chlorogenic acid, aucubin reduce.
(2) one grade fermemtation: access the wine activated yeast of activation by the 0.1-0.15% of fermenation raw liquid weight, ferments 5-10 days in 20-28 DEG C, makes Cortex Eucommiae hesperidium aurantium wine, enters apparatus for acetic acid fermentation tank after filtration.
(3) second order fermentation: add the acetic acid bacterium solution of 8-12% domestication in Cortex Eucommiae hesperidium aurantium wine, carry out a day 2-3 time, the air agitation of each 1 minute, make fruit wine fully contact with oxygen, mixing time 3-4 days, temperature controls at 26-33 DEG C, obtains Cortex Eucommiae fermentation health preserving vinegar.
Utilizing the present invention to produce the Cortex Eucommiae fermentation health preserving vinegar obtained, form is full, and good looking appearance is sepia, and fragrance is high thoroughly, and mellow in taste, gives off a strong fragrance, and without astringent taste and the bitterness of Folium Eucommiae, has the Cortex Eucommiae fermentation distinctive local flavor of health preserving vinegar.Testing through function test, due to the addition of Folium Eucommiae extracting solution, this product is for hypertension, hyperlipidemia, and enhancing gastrointestinal function all has preferable auxiliary curative effect.
Compared with prior art, beneficial effects of the present invention also resides in:
Folium Eucommiae utilizes compound enzymic preparation to carry out enzymolysis before extracting, by the cell wall damage of Folium Eucommiae, enable effective ingredient Fast Stripping, improve the extraction ratio of the effective ingredient such as chlorogenic acid, aucubin, and the compound enzymic preparation of the present invention is an optimum organization, for the feature of Folium Eucommiae cell wall main component, design cellulase mass fraction 60-70%, pectase mass fraction is 15-25%, and remaining is xylanase, makes hydrolysis result optimal.
During the reflux, extract, of Folium Eucommiae, carry out evacuation and vacuum breaker, utilizing vacuum impulse to strengthen mass transfer, make the effective ingredient dissolutions to greatest extent such as the chlorogenic acid in Folium Eucommiae histiocyte, aucubin, the Cortex Eucommiae fermentation health preserving vinegar function making follow-up making is optimal.
What the present invention taked is the production technology will fermented again after Folium Eucommiae extracting solution and the mixing of Citrus chachiensis Hort. juice, compared with the production technology remixing allotment Folium Eucommiae extracting solution after conventional fruit juice fermentation, this technique is during the fermentation due to the effect of microorganism, can effectively decompose the tannin in Folium Eucommiae, thus eliminate astringent taste and the bitterness of product, strengthen the mouthfeel of product, improve market adaptability.
The present invention utilizes the acetic acid bacterium solution after domestication to carry out second order fermentation, eliminates the inhibitory action of antimicrobial component Dichlorodiphenyl Acetate bacterium in Folium Eucommiae to greatest extent, makes acetic fermentation smooth, and effectively save fermentation time, reduce cost.
In sum, the present invention is by carrying out effective integration by above multinomial technology, it is achieved that make the optimum efficiency of Cortex Eucommiae fermentation health preserving vinegar with Folium Eucommiae, Citrus chachiensis Hort. juice, and the market competitiveness is strong, has a extensive future.
Detailed description of the invention
Hereinafter Cortex Eucommiae fermentation health preserving vinegar and preparation method thereof is described in detail.
Embodiment 1:
(1) fermenation raw liquid makes: by hesperidium aurantium meat broken juice, coarse filtration, entrance turbid juice tank, add the Folium Eucommiae extracting solution of Citrus chachiensis Hort. juice volume 12%, then the sugar content adjusting Citrus chachiensis Hort. juice is 15.8%, at 75 DEG C, pasteurization under conditions of 15 minutes, make fermenation raw liquid, be delivered to ethanol fermentation tank.
The preparation method of described Folium Eucommiae extracting solution is: toward being broken into the enzymolysis solution adding 9 times amount in the 25 dry Folium Eucommiaes of purpose, enzymolysis 3.5 hours at a temperature of 42 degree, after enzymolysis completes, the edible ethanol of addition 95% is until the volume fraction of ethanol is 27%, carry out reflux, extract, 2.5 hours extraction times, after having extracted, it is evaporated to produce precipitate, standby.
Enzymolysis solution preparation method is: is dissolved in the buffer that 500ml pH value is 6.5 by 13 grams of compound enzymic preparations and get final product.The proportioning of compound enzymic preparation is cellulase mass fraction 62%, and pectase mass fraction is 22%, and remaining is xylanase, and the average enzyme work of compound enzymic preparation is 22000 units.
Extraction pot can be carried out step evacuation and vacuum breaker during said extracted, utilize vacuum impulse to strengthen mass transfer.
(2) one grade fermemtation: by the wine activated yeast of 0.11% access activation of fermenation raw liquid weight, ferment 6 days in 27 DEG C, make Cortex Eucommiae hesperidium aurantium wine, enter apparatus for acetic acid fermentation tank after filtration.
(3) second order fermentation: add the acetic acid bacterium solution of 9% domestication in Cortex Eucommiae hesperidium aurantium wine, carry out a day 3 times, the air agitation of each 1 minute, make fruit wine fully contact with oxygen, mixing time 3 days, temperature controls at 32 DEG C, obtains Cortex Eucommiae fermentation health preserving vinegar.
Utilizing the present invention to produce the Cortex Eucommiae fermentation health preserving vinegar obtained, form is full, and good looking appearance is sepia, and fragrance is high thoroughly, and mellow in taste, gives off a strong fragrance, and without astringent taste and the bitterness of Folium Eucommiae, has the Cortex Eucommiae fermentation distinctive local flavor of health preserving vinegar.Testing through function test, due to the addition of Folium Eucommiae extracting solution, this product is for hypertension, hyperlipidemia, and enhancing gastrointestinal function all has preferable auxiliary curative effect.
Embodiment 2:
(1) fermenation raw liquid makes: by hesperidium aurantium meat broken juice, coarse filtration, entrance turbid juice tank, add the Folium Eucommiae extracting solution of Citrus chachiensis Hort. juice volume 18%, then the sugar content adjusting Citrus chachiensis Hort. juice is 15.1%, at 75 DEG C, pasteurization under conditions of 15 minutes, make fermenation raw liquid, be delivered to ethanol fermentation tank.
The preparation method of described Folium Eucommiae extracting solution is: toward being broken into the enzymolysis solution adding 7 times amount in the 35 dry Folium Eucommiaes of purpose, enzymolysis 1.5 hours at a temperature of 48 degree, after enzymolysis completes, the edible ethanol of addition 95% is until the volume fraction of ethanol is 22%, carry out reflux, extract, 5.4 hours extraction times, after having extracted, it is evaporated to produce precipitate, standby.
Enzymolysis solution preparation method is: is dissolved in the buffer that 500ml pH value is 4.8 by 2 grams of compound enzymic preparations and get final product.The proportioning of compound enzymic preparation is cellulase mass fraction 68%, and pectase mass fraction is 16%, and remaining is xylanase, and the average enzyme work of compound enzymic preparation is 27000 units.
Extraction pot can be carried out step evacuation and vacuum breaker during said extracted, utilize vacuum impulse to strengthen mass transfer.
(2) one grade fermemtation: by the wine activated yeast of 0.14% access activation of fermenation raw liquid weight, ferment 9 days in 22 DEG C, make Cortex Eucommiae hesperidium aurantium wine, enter apparatus for acetic acid fermentation tank after filtration;
(3) second order fermentation: add the acetic acid bacterium solution of 11% domestication in Cortex Eucommiae hesperidium aurantium wine, carry out a day 2 times, the air agitation of each 1 minute, make fruit wine fully contact with oxygen, mixing time 4 days, temperature controls at 28 DEG C, obtains Cortex Eucommiae fermentation health preserving vinegar.
Utilizing the present invention to produce the Cortex Eucommiae fermentation health preserving vinegar obtained, form is full, and good looking appearance is sepia, and fragrance is high thoroughly, and mellow in taste, gives off a strong fragrance, and without astringent taste and the bitterness of Folium Eucommiae, has the Cortex Eucommiae fermentation distinctive local flavor of health preserving vinegar.Testing through function test, due to the addition of Folium Eucommiae extracting solution, this product is for hypertension, hyperlipidemia, and enhancing gastrointestinal function all has preferable auxiliary curative effect.
The present invention is not made any pro forma restriction by above-described embodiment, any those of ordinary skill in the art, in the case of without departing from technical solution of the present invention scope, technical solution of the present invention is made many possible variations and modification by the technology contents that all may utilize the disclosure above, or is revised as the Equivalent embodiments of equivalent variations.Therefore, every content without departing from technical solution of the present invention, according to the technology of the present invention essence to any simple modification made for any of the above embodiments, equivalent variations and modification, all should fall in the range of technical solution of the present invention is protected.
Claims (7)
1. the preparation method of Cortex Eucommiae fermentation health preserving vinegar, it is characterised in that comprise following step:
(1) fermenation raw liquid makes: by hesperidium aurantium meat broken juice, coarse filtration, entrance turbid juice tank, add the Folium Eucommiae extracting solution of Citrus chachiensis Hort. juice volume 10%-20%, then the sugar content adjusting Citrus chachiensis Hort. juice is 15-16%, at 75 DEG C, pasteurization under conditions of 15 minutes, make fermenation raw liquid, be delivered to ethanol fermentation tank;
(2) one grade fermemtation: access the wine activated yeast of activation by the 0.1-0.15% of fermenation raw liquid weight, ferments 5-10 days in 20-28 DEG C, makes Cortex Eucommiae hesperidium aurantium wine, enters apparatus for acetic acid fermentation tank after filtration;
(3) second order fermentation: add the acetic acid bacterium solution of 8-12% domestication in Cortex Eucommiae hesperidium aurantium wine, carry out a day 2-3 time, the air agitation of each 1 minute, make fruit wine fully contact with oxygen, mixing time 3-4 days, temperature controls at 26-33 DEG C, obtains Cortex Eucommiae fermentation health preserving vinegar.
The preparation method of Cortex Eucommiae fermentation health preserving vinegar the most according to claim 1, it is characterized in that: the preparation method of described Folium Eucommiae extracting solution is: toward being broken in the dry Folium Eucommiae of 20-40 purpose the enzymolysis solution adding 6-10 times amount, enzymolysis 1-4 hour at a temperature of 40-50 degree, after enzymolysis completes, the edible ethanol of addition 95%, until the volume fraction of ethanol is 20%-30%, carries out reflux, extract, 2-6 hour extraction time, extraction is evaporated to produce precipitate after completing, standby.
The preparation method of Cortex Eucommiae fermentation health preserving vinegar the most according to claim 2, it is characterised in that: described enzymolysis solution preparation method is: is dissolved in the buffer that 500ml pH value is 4-7 by 1-15 gram of compound enzymic preparation and get final product.
The preparation method of Cortex Eucommiae fermentation health preserving vinegar the most according to claim 3, it is characterised in that: the proportioning of described compound enzymic preparation is cellulase mass fraction 60-70%, pectase mass fraction 15-25%, and remaining is xylanase.
The preparation method of Cortex Eucommiae fermentation health preserving vinegar the most according to claim 4, it is characterised in that: the average enzyme of described compound enzymic preparation is lived as 20000-30000 unit.
The preparation method of Cortex Eucommiae fermentation health preserving vinegar the most according to claim 5, it is characterised in that: during Folium Eucommiae reflux, extract, extraction pot is carried out step evacuation and vacuum breaker, utilize vacuum impulse to strengthen mass transfer.
7. the Cortex Eucommiae fermentation health preserving vinegar obtained according to method described in claim 1 or 2 or 3 or 4 or 5 or 6, it is characterised in that: Cortex Eucommiae fermentation health preserving vinegar is for hypertension, hyperlipidemia, and enhancing gastrointestinal function all has certain auxiliary curative effect.
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106578821A (en) * | 2016-12-19 | 2017-04-26 | 合肥徽徽逗食品有限公司 | Ginger solid drink with effects of nourishing kidney and strengthening essence and preparation method thereof |
CN106605821A (en) * | 2016-12-19 | 2017-05-03 | 合肥徽徽逗食品有限公司 | Fresh ginger solid beverage for preventing cancer and preparation method of fresh ginger solid beverage |
CN106605820A (en) * | 2016-12-19 | 2017-05-03 | 合肥徽徽逗食品有限公司 | Fresh ginger solid beverage for benefitting bodies of people with consumptive diseases and preparation method of fresh ginger solid beverage |
CN106616181A (en) * | 2016-12-19 | 2017-05-10 | 合肥徽徽逗食品有限公司 | Antioxidant ginger solid drink and preparation method thereof |
CN106616180A (en) * | 2016-12-19 | 2017-05-10 | 合肥徽徽逗食品有限公司 | Stomach-warming fresh ginger solid beverage and preparation method thereof |
CN106689957A (en) * | 2016-12-19 | 2017-05-24 | 合肥徽徽逗食品有限公司 | Fresh ginger solid beverage for inducing diuresis for removing edema and preparation method of fresh ginger solid beverage |
CN106720819A (en) * | 2016-12-19 | 2017-05-31 | 合肥徽徽逗食品有限公司 | One kind is refreshed oneself QI invigorating power solid ginger beverage and preparation method thereof |
CN106720388A (en) * | 2016-12-19 | 2017-05-31 | 合肥徽徽逗食品有限公司 | A kind of solid ginger beverage of anti-bacteria and anti-virus and preparation method thereof |
CN108576320A (en) * | 2018-05-08 | 2018-09-28 | 吉首大学 | A kind of preparation method of Cortex Eucommiae tea vinegar drink |
CN113186070A (en) * | 2021-04-02 | 2021-07-30 | 完美(广东)日用品有限公司 | Gynura procumbens vinegar and preparation method thereof |
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CN106578821A (en) * | 2016-12-19 | 2017-04-26 | 合肥徽徽逗食品有限公司 | Ginger solid drink with effects of nourishing kidney and strengthening essence and preparation method thereof |
CN106605821A (en) * | 2016-12-19 | 2017-05-03 | 合肥徽徽逗食品有限公司 | Fresh ginger solid beverage for preventing cancer and preparation method of fresh ginger solid beverage |
CN106605820A (en) * | 2016-12-19 | 2017-05-03 | 合肥徽徽逗食品有限公司 | Fresh ginger solid beverage for benefitting bodies of people with consumptive diseases and preparation method of fresh ginger solid beverage |
CN106616181A (en) * | 2016-12-19 | 2017-05-10 | 合肥徽徽逗食品有限公司 | Antioxidant ginger solid drink and preparation method thereof |
CN106616180A (en) * | 2016-12-19 | 2017-05-10 | 合肥徽徽逗食品有限公司 | Stomach-warming fresh ginger solid beverage and preparation method thereof |
CN106689957A (en) * | 2016-12-19 | 2017-05-24 | 合肥徽徽逗食品有限公司 | Fresh ginger solid beverage for inducing diuresis for removing edema and preparation method of fresh ginger solid beverage |
CN106720819A (en) * | 2016-12-19 | 2017-05-31 | 合肥徽徽逗食品有限公司 | One kind is refreshed oneself QI invigorating power solid ginger beverage and preparation method thereof |
CN106720388A (en) * | 2016-12-19 | 2017-05-31 | 合肥徽徽逗食品有限公司 | A kind of solid ginger beverage of anti-bacteria and anti-virus and preparation method thereof |
CN108576320A (en) * | 2018-05-08 | 2018-09-28 | 吉首大学 | A kind of preparation method of Cortex Eucommiae tea vinegar drink |
CN113186070A (en) * | 2021-04-02 | 2021-07-30 | 完美(广东)日用品有限公司 | Gynura procumbens vinegar and preparation method thereof |
CN113186070B (en) * | 2021-04-02 | 2022-12-06 | 完美(广东)日用品有限公司 | Gynura procumbens vinegar and preparation method thereof |
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Application publication date: 20161012 |