CN106009066A - Method for preparing nanometer materials with konjac glucomannan and chitosan - Google Patents
Method for preparing nanometer materials with konjac glucomannan and chitosan Download PDFInfo
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- CN106009066A CN106009066A CN201610432069.0A CN201610432069A CN106009066A CN 106009066 A CN106009066 A CN 106009066A CN 201610432069 A CN201610432069 A CN 201610432069A CN 106009066 A CN106009066 A CN 106009066A
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- chitosan
- turbid liquid
- nano material
- rhizoma amorphophalli
- amorphophalli glucomannan
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- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 title claims abstract description 36
- 229920002581 Glucomannan Polymers 0.000 title claims abstract description 31
- 229940046240 glucomannan Drugs 0.000 title claims abstract description 31
- 229920001661 Chitosan Polymers 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000000463 material Substances 0.000 title abstract description 21
- 241001312219 Amorphophallus konjac Species 0.000 title abstract 3
- 235000001206 Amorphophallus rivieri Nutrition 0.000 title abstract 3
- 229920002752 Konjac Polymers 0.000 title abstract 3
- 235000010485 konjac Nutrition 0.000 title abstract 3
- 239000000252 konjac Substances 0.000 title abstract 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000008157 edible vegetable oil Substances 0.000 claims abstract description 15
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000005406 washing Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- RYYKJJJTJZKILX-UHFFFAOYSA-M sodium octadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC([O-])=O RYYKJJJTJZKILX-UHFFFAOYSA-M 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000004132 cross linking Methods 0.000 claims abstract description 8
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 7
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 7
- 235000019832 sodium triphosphate Nutrition 0.000 claims abstract description 7
- 238000004945 emulsification Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 45
- 239000002086 nanomaterial Substances 0.000 claims description 42
- 238000002156 mixing Methods 0.000 claims description 12
- 239000003921 oil Substances 0.000 claims description 7
- 238000004513 sizing Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 18
- 239000002245 particle Substances 0.000 abstract description 7
- 238000001035 drying Methods 0.000 abstract description 5
- 230000010355 oscillation Effects 0.000 abstract description 5
- 239000000243 solution Substances 0.000 abstract 3
- 239000000969 carrier Substances 0.000 abstract 1
- SWXVUIWOUIDPGS-UHFFFAOYSA-N diacetone alcohol Natural products CC(=O)CC(C)(C)O SWXVUIWOUIDPGS-UHFFFAOYSA-N 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 239000011259 mixed solution Substances 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000003013 cytotoxicity Effects 0.000 description 8
- 231100000135 cytotoxicity Toxicity 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 229940079593 drug Drugs 0.000 description 6
- 239000003431 cross linking reagent Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 231100001083 no cytotoxicity Toxicity 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 2
- 239000003094 microcapsule Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 235000010413 sodium alginate Nutrition 0.000 description 2
- 229940005550 sodium alginate Drugs 0.000 description 2
- 239000000661 sodium alginate Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 229940116364 hard fat Drugs 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/02—Dextran; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
- C08J3/246—Intercrosslinking of at least two polymers
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/02—Dextran; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/02—Dextran; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Inorganic Chemistry (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a method for preparing nanometer materials with konjac glucomannan and chitosan. The method comprises the specific steps that an acetic acid solution of konjac glucomannan and chitosan is added into a mixed solution of edible oil and water, then Tween-80, a sodium tripolyphosphate solution and a sodium stearate solution are added in sequence, the temperature and the cross-linking time are controlled, sufficient emulsification cross-linking is carried out, then washing is carried out with acetone and ethyl alcohol in sequence, ultrasonic oscillation, screening and drying are carried out, and the needed nanometer materials are obtained. According to the nanometer materials prepared with the method, the particle size ranges from 800 nm to 900 nm, grooves are formed in the surfaces of the materials, and the nanometer materials can serve as medicine carriers.
Description
Technical field
The present invention relates to a kind of Rhizoma amorphophalli glucomannan and the method that nano material prepared by chitosan, belong to material preparation neck
Territory.
Background technology
Nano material technology has huge potentiality in material science and the aspect such as fluorescent optical sensor, biomedical detection,
Prepare nano material with nanotechnology and the impact of pharmacodynamics has been caused the great attention of the world of medicine, the average particle of nano material
The less specific surface area making nano material in footpath increases, and the increase of specific surface area makes surface energy and the surface activity of nano material
Increasing, need the mode physically or chemically adsorbed to reduce its surface energy, the surface of such nano material is easy for former with other
Son reacts.
Chitosan has the polycation characteristic of uniqueness, can be with sodium alginate (polyanion) by electrostatic phase interaction
With, at sodium alginate microcapsule surface attachment one strata electrolyte semipermeable membrane, thus improve the stability of microcapsule.Chitosan belongs to hydrophilic
Property polymer, can prepare different size of material, material and medicine cohesive process can be avoided the use of organic solvent, stop
The degeneration of antigen protein；Chitosan material has loose structure, and this makes antigen protein not only can be adsorbed on material bodies
Surface, and portion can be embedded within；Chitosan material can also control the release of protein drug, improves antigen protein
Absorption.
Rhizoma amorphophalli glucomannan (KGM), because the structure of its uniqueness, has good water solublity, water-retaining property, thickening property and glue
Solidifying property, the most also has emulsifying, suspends, the characteristic such as stablizes.The bio-modification technology of Rhizoma amorphophalli glucomannan (KGM) is mainly enzyme process
Modification, by corresponding polyase effect, makes the space structure of Rhizoma amorphophalli glucomannan (KGM) that corresponding change to occur, makes long
Rhizoma amorphophalli glucomannan (KGM) strand is hydrolyzed to short Rhizoma amorphophalli glucomannan (KGM) strand, though Rhizoma amorphophalli glucomannan
(KGM) transfer oligosaccharide or oligosaccharide to saccharide portion.
Summary of the invention
A kind of method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material, specifically includes following steps:
(1) Rhizoma amorphophalli glucomannan and chitosan are mixed, join in the acetic acid solution that mass fraction is 5%, be sufficiently stirred for,
To turbid liquid A；
(2) stir after taking edible oil and water mixing, configure 30mL edible oil solution for later use；
(3) take 3-6mL turbid liquid A to add in the edible oil solution that step (2) obtains, obtain turbid liquid B；
(4) in turbid liquid B, 3-7mL tween 80 is added, fully emulsified prepared turbid liquid C；
(5) in turbid liquid C, it is initially charged the sodium tripolyphosphate solution that 3-6mL mass fraction is 8%, adds 3-6mL mass fraction
The sodium stearate solution of 0.3%, fully emulsified crosslinking obtains turbid liquid D；
(6) successively with acetone, washing with alcohol turbid liquid D, more ultrasonic 6-15min, final sizing, dried, obtain nano material.
Preferably, the mass ratio of step (1) described Rhizoma amorphophalli glucomannan and chitosan is 1 0.8-1 1.5, acetic acid solution
Amount and Rhizoma amorphophalli glucomannan and the ratio of gross mass of chitosan be 40-60mL/g.
Preferably, step (2) described water oil volume ratio is 1 4-1 8, and mixing speed is 900-1500r/min.
Preferably, step (5) told emulsification and cross linked temperature is 45-75 DEG C, and the time is 1.5-4h.
The present invention is to utilize Rhizoma amorphophalli glucomannan and chitosan to prepare nano material, and the particle diameter of nano material is 800-
900nm, and material balling ratio is good, material surface is fluted, can use as pharmaceutical carrier；This method raw material is the easiest
, preparation process technique is simple and environmentally-friendly.
Accompanying drawing explanation
Fig. 1 is the scanning electron microscopic picture of the nano material that the embodiment of the present invention 1 prepares；
Fig. 2 is the drug release profiles of the nano material medicine carrying that the embodiment of the present invention 2 prepares；
Fig. 3 is the scanning electron microscopic picture of the nano material that the embodiment of the present invention 4 prepares.
Detailed description of the invention
With specific embodiment, the present invention is described in further detail below in conjunction with the accompanying drawings, but protection scope of the present invention is also
It is not limited to described content.
Embodiment 1
The method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material described in the present embodiment, specifically includes following steps:
(1) it is the ratio of 1 0.8 according to mass ratio, weighs 1g Rhizoma amorphophalli glucomannan and the mixing of 0.8g chitosan respectively, join
72mL mass fraction is in the acetic acid solution of 5%, is sufficiently stirred for, and obtains turbid liquid A；
(2) according to the ratio that water oil volume ratio is 14, taking Oleum Brassicae campestris and water mix and blend, mixing speed is 900r/min, configuration
30mL edible oil solution for later use；
(3) in the edible oil solution configured, add the configured good turbid liquid A of 5mL with syringe, obtain turbid liquid B；
(4) in turbid liquid B, 3mL tween 80 is added, fully emulsified prepared turbid liquid C；
(5) being initially charged the sodium tripolyphosphate solution that 3mL mass fraction is 8% in turbid liquid C, adding 5mL mass fraction is 0.3%
Sodium stearate solution, control temperature be 45 DEG C, the time is 1.5h, and fully emulsified crosslinking obtains turbid liquid D；
(6) with washing with acetone three times to remove cross-linking agent and sodium stearate, then with washing with alcohol three times, then sonic oscillation
6min, last 800 mesh sieves divide, and after drying, obtain nano material.
Fig. 1 show the scanning electron microscopic picture of the nano material of embodiment 1 preparation, can be with the particle diameter of nano material from figure
Between 810-900nm, material surface has some grooves.
Standard GB/T/T14233.3-2005 regulation: (1) concentration when 0.05-0.3g/mL, relative growth rate (RGR)
All between 90-110, cytotoxicity is 0 or 1 grade；(2) concentration is when 0.05-0.2g/mL, and relative growth rate (RGR) all exists
Between 100-112, cytotoxicity is 0 grade；Concentration when 0.2-0.4g/mL, relative growth rate all between 75-100, cell toxicant
Property is 1 grade；(3) support concentration is when 0.05-0.2g/mL, and relative growth rate (RGR) is all between 75-95, and cytotoxicity is 1
Level；Concentration is when 0.2-0.4g/mL, and relative growth rate (RGR) is all between 95-120, and cytotoxicity is 0 grade or 1 grade；To reality
The nano material executing example 1 gained carries out cytotoxicity experiment, the results are shown in Table 1, shows this material no cytotoxicity.
The cell experiment of table 1 nano material
。
Embodiment 2
The method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material described in the present embodiment, specifically includes following steps:
(1) it is the ratio of 11 according to mass ratio, weighs 1g Rhizoma amorphophalli glucomannan and the mixing of 1g chitosan respectively, join 100mL
Mass fraction is in the acetic acid solution of 5%, is sufficiently stirred for, and obtains turbid liquid A；
(2) being the ratio of 17 according to water-oil factor, take soybean oil and water mix and blend, mixing speed is 1400r/min, configuration
30mL edible oil solution for later use；
(3) in the edible oil solution configured, add the configured good turbid liquid A of 3mL with syringe, obtain turbid liquid B；
(4) in turbid liquid B, 7mL tween 80 is added, fully emulsified prepared turbid liquid C；
(5) adding 6mL mass fraction in turbid liquid C is the sodium tripolyphosphate solution of 8%, and adding 6mL mass fraction is 0.3%
Sodium stearate solution, controlling temperature is 55 DEG C, and the time is 4h, obtains turbid liquid D after fully emulsified crosslinking；
(6) with washing with acetone three times to remove cross-linking agent and sodium stearate, then with washing with alcohol three times, then sonic oscillation
15min, last 800 mesh sieves divide, and after drying, obtain nano material.
The Electronic Speculum test that is scanned through of the nano material of embodiment 2 preparation show that the particle diameter of nano material is at 800-900nm
Between, material surface is fluted；The method using embodiment 1 carries out cytotoxicity experiment, and result shows prepared by the present embodiment
Nano material no cytotoxicity.
The drug release experiment of the medicine carrying of the nano material of embodiment 2 preparation is as shown in Figure 2, it is known that medicine is at 0.5h to 5h
In the range of drug release rate big, prolongation over time, drug release percentage ratio increases, the drug release when the time reaches 12h
Percentage ratio is 99.2%, and the medicine not discharged only has 0.2% residual in the material, illustrates that medicine is slowly release, serves slow
The effect released, and be almost all release, reach the purpose of medicine carrying.
Embodiment 3
The method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material described in the present embodiment, specifically includes following steps:
(1) it is the ratio of 1 1.5 according to mass ratio, weighs 1g Rhizoma amorphophalli glucomannan and the mixing of 1.5g chitosan respectively, join
150mL mass fraction is in the acetic acid solution of 5%, is sufficiently stirred for, and obtains turbid liquid A；
(2) being the ratio of 15 according to water-oil factor, take Oleum Arachidis hypogaeae semen and water mix and blend, mixing speed is 1500r/min, configuration
30mL edible oil solution for later use；
(3) in the edible oil solution configured, add the configured good turbid liquid A of 4mL with syringe, obtain turbid liquid B；
(4) in turbid liquid B, 6mL tween 80 is added, fully emulsified prepared turbid liquid C；
(5) being initially charged the sodium tripolyphosphate solution that 4mL mass fraction is 8% in turbid liquid C, adding 3mL mass fraction is 0.3%
Sodium stearate solution, control temperature be 60 DEG C, the time is 2.5h, obtains turbid liquid D after fully emulsified crosslinking；
(6) with washing with acetone three times to remove cross-linking agent and sodium stearate, then with washing with alcohol three times, then sonic oscillation
12min, last 800 mesh sieves divide, and after drying, obtain nano material.
The Electronic Speculum test that is scanned through of the nano material of embodiment 3 preparation show that the particle diameter of nano material is at 800-890nm
Between, material surface is fluted；The method using embodiment 1 carries out cytotoxicity experiment, and result shows prepared by the present embodiment
Nano material no cytotoxicity；The method using embodiment 2 carries out medicine carrying extracorporeal releasing experiment, and result display medicine is slowly to release
Put, serve the effect of slow release, and be almost all release, reached the purpose of medicine carrying.
Embodiment 4
The method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material described in the present embodiment, specifically includes following steps:
(1) it is the ratio of 1 1.2 by mass ratio, weighs 1g Rhizoma amorphophalli glucomannan and the mixing of 1.2g chitosan respectively, join
99mL mass fraction is in the acetic acid solution of 5%, is sufficiently stirred for, and obtains turbid liquid A；
(2) being the ratio of 18 according to water-oil factor, take Semen Maydis oil and water mix and blend, mixing speed is 1300r/min, configuration
30mL edible oil solution for later use；
(3) in the edible oil solution configured, add the configured good turbid liquid A of 5mL with syringe, obtain turbid liquid B；
(4) in turbid liquid B, 5mL tween 80 is added, fully emulsified prepared turbid liquid C；
(5) in turbid liquid C add 5mL mass fraction be the sodium tripolyphosphate solution of 8%, then add 4mL mass fraction be 0.3% hard
Fat acid sodium solution, controlling temperature is 75 DEG C, and the time is 3h, and fully emulsified crosslinking obtains turbid liquid D；
(6) with washing with acetone three times to remove cross-linking agent and sodium stearate, then with washing with alcohol three times, then sonic oscillation
10min, last 800 mesh sieves divide, and after drying, obtain nano material.
Be illustrated in figure 3 embodiment 4 preparation nano material be scanned through electron microscopic picture, draw the particle diameter of nano material
Between 840-900nm, material surface is fluted；The method using embodiment 1 carries out cytotoxicity experiment, and result shows this reality
Execute nano material no cytotoxicity prepared by example.
Claims (4)
1. one kind utilizes the method that nano material prepared by Rhizoma amorphophalli glucomannan and chitosan, it is characterised in that specifically include following
Step:
(1) Rhizoma amorphophalli glucomannan and chitosan are mixed, join in the acetic acid solution that mass fraction is 5%, be sufficiently stirred for,
To turbid liquid A；
(2) stir after taking edible oil and water mixing, configure 30mL edible oil solution for later use；
(3) take 3-6mL turbid liquid A to add in the edible oil solution that step (2) obtains, obtain turbid liquid B；
(4) in turbid liquid B, 3-7mL tween 80 is added, fully emulsified prepared turbid liquid C；
(5) in turbid liquid C, it is initially charged the sodium tripolyphosphate solution of 3-6mL mass fraction 8%, adds 3-6mL mass fraction 0.3%
Sodium stearate solution, fully emulsified crosslinking obtains turbid liquid D；
(6) successively with acetone, washing with alcohol turbid liquid D, more ultrasonic 6-15min, final sizing, dried, obtain nano material.
The method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material the most according to claim 1, its feature exists
In, the mass ratio of step (1) described Rhizoma amorphophalli glucomannan and chitosan is 1 0.8-1 1.5, mass fraction be 5% acetic acid molten
The amount of liquid is 40-60mL/g with the ratio of Rhizoma amorphophalli glucomannan and the gross mass of chitosan.
The method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material the most according to claim 1, its feature exists
In, the water oil volume ratio in step (2) is 1 4-1 8, and mixing speed is 900-1500r/min.
The method utilizing Rhizoma amorphophalli glucomannan and chitosan to prepare nano material the most according to claim 1, its feature exists
In, the emulsification and cross linked temperature of step (5) is 45-75 DEG C, and the time is 1.5-4h.
Priority Applications (1)
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